AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76156

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76156.t1 MTR_2g099780 94.866 409 14 2 1 407 1 404 0.0 685
MS.gene76156.t1 MTR_8g017570 53.499 443 158 14 1 406 1 432 1.82e-109 329
MS.gene76156.t1 MTR_3g104570 46.606 442 182 14 1 398 1 432 2.65e-84 265
MS.gene76156.t1 MTR_4g050480 40.959 271 135 6 1 256 1 261 1.20e-37 141
MS.gene76156.t1 MTR_4g050480 39.858 281 125 6 1 256 1 262 1.05e-36 139
MS.gene76156.t1 MTR_6g033415 36.304 303 154 7 79 352 105 397 1.98e-29 119
MS.gene76156.t1 MTR_8g024540 41.451 193 85 7 79 256 125 304 1.41e-28 117
MS.gene76156.t1 MTR_1g095740 37.989 179 99 5 80 255 140 309 4.58e-27 112
MS.gene76156.t1 MTR_1g066800 39.896 193 98 7 58 248 99 275 9.47e-27 111
MS.gene76156.t1 MTR_1g066800 39.896 193 98 7 58 248 104 280 1.36e-26 111
MS.gene76156.t1 MTR_7g100790 31.579 342 163 10 55 345 88 409 7.18e-26 109
MS.gene76156.t1 MTR_1g103070 29.134 254 102 6 1 181 1 249 1.09e-21 97.1
MS.gene76156.t1 MTR_0047s0040 38.060 134 74 3 80 207 100 230 2.47e-21 95.9
MS.gene76156.t1 MTR_2g078210 34.659 176 99 4 83 255 16 178 2.91e-21 92.8
MS.gene76156.t1 MTR_2g078210 34.659 176 99 4 83 255 76 238 3.00e-21 94.0
MS.gene76156.t1 MTR_2g015370 34.270 178 105 3 83 260 65 230 7.17e-21 92.4
MS.gene76156.t1 MTR_8g014580 32.500 200 74 4 1 147 1 192 2.85e-20 92.4
MS.gene76156.t1 MTR_3g020990 41.593 113 58 2 94 205 103 208 1.80e-19 87.4
MS.gene76156.t1 MTR_1g007920 32.867 286 169 7 79 344 95 377 2.55e-19 90.1
MS.gene76156.t1 MTR_1g007920 32.867 286 169 7 79 344 120 402 2.62e-19 90.1
MS.gene76156.t1 MTR_1g041430 33.333 228 100 6 1 179 1 225 9.39e-19 88.2
MS.gene76156.t1 MTR_4g108140 33.523 176 93 3 1 155 1 173 1.15e-18 88.6
MS.gene76156.t1 MTR_4g108140 34.463 177 91 4 1 155 1 174 1.18e-18 88.6
MS.gene76156.t1 MTR_3g091560 38.596 171 91 4 81 247 112 272 3.06e-18 86.7
MS.gene76156.t1 MTR_4g102840 36.486 148 87 2 1 141 1 148 1.59e-17 84.3
MS.gene76156.t1 MTR_0019s0070 30.240 334 170 14 83 376 119 429 1.88e-17 84.3
MS.gene76156.t1 MTR_8g074730 53.623 69 32 0 76 144 66 134 2.92e-17 78.6
MS.gene76156.t1 MTR_8g102400 39.205 176 76 6 1 145 1 176 5.49e-17 82.8
MS.gene76156.t1 MTR_4g087420 31.724 145 83 2 77 209 142 282 5.84e-17 83.2
MS.gene76156.t1 MTR_2g100740 34.043 188 98 6 1 162 1 188 6.99e-17 81.6
MS.gene76156.t1 MTR_7g114870 31.148 183 83 5 1 149 1 174 2.79e-16 80.5
MS.gene76156.t1 MTR_1g051810 26.733 303 169 11 83 372 107 369 3.30e-16 80.1
MS.gene76156.t1 MTR_1g101330 56.061 66 29 0 76 141 110 175 4.53e-16 80.1
MS.gene76156.t1 MTR_7g114700 50.000 66 33 0 80 145 134 199 3.15e-15 77.4
MS.gene76156.t1 MTR_5g038620 38.614 101 62 0 59 159 97 197 3.58e-13 71.6
MS.gene76156.t1 MTR_1g023760 50.746 67 33 0 81 147 124 190 8.41e-13 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76156.t1 AT1G72670 46.336 423 167 17 1 391 1 395 1.25e-88 275
MS.gene76156.t1 AT2G26180 47.826 414 177 12 1 388 1 401 1.34e-83 262
MS.gene76156.t1 AT1G17480 38.830 376 154 13 1 342 1 334 3.92e-61 202
MS.gene76156.t1 AT5G03040 40.394 203 111 5 52 252 85 279 1.19e-32 128
MS.gene76156.t1 AT5G03040 40.394 203 111 5 52 252 85 279 1.19e-32 128
MS.gene76156.t1 AT5G03040 40.394 203 111 5 52 252 85 279 1.19e-32 128
MS.gene76156.t1 AT3G22190 48.901 182 77 4 79 256 86 255 6.32e-32 125
MS.gene76156.t1 AT3G22190 48.901 182 77 4 79 256 86 255 6.32e-32 125
MS.gene76156.t1 AT3G22190 48.901 182 77 4 79 256 86 255 6.32e-32 125
MS.gene76156.t1 AT3G22190 48.901 182 77 4 79 256 86 255 6.32e-32 125
MS.gene76156.t1 AT1G01110 36.232 207 109 5 71 263 111 308 6.88e-27 112
MS.gene76156.t1 AT4G00820 38.095 189 89 5 79 251 130 306 3.36e-26 110
MS.gene76156.t1 AT3G52290 37.572 173 97 4 80 251 107 269 6.04e-25 106
MS.gene76156.t1 AT3G49260 29.553 291 157 10 83 344 122 393 9.95e-25 106
MS.gene76156.t1 AT3G49260 29.553 291 156 11 83 344 122 392 1.26e-24 105
MS.gene76156.t1 AT3G49260 29.553 291 156 11 83 344 122 392 1.26e-24 105
MS.gene76156.t1 AT3G49260 29.553 291 156 11 83 344 122 392 1.26e-24 105
MS.gene76156.t1 AT3G09710 37.017 181 99 6 79 256 106 274 2.01e-24 105
MS.gene76156.t1 AT3G09710 34.896 192 102 5 79 256 106 288 6.59e-24 103
MS.gene76156.t1 AT4G10640 29.319 382 187 14 7 345 18 359 4.20e-23 100
MS.gene76156.t1 AT4G14750 28.521 284 155 9 1 249 1 271 1.23e-22 99.0
MS.gene76156.t1 AT4G14750 28.521 284 155 9 1 249 60 330 1.75e-22 99.4
MS.gene76156.t1 AT4G14750 28.521 284 155 9 1 249 60 330 1.89e-22 99.4
MS.gene76156.t1 AT3G59690 37.324 142 75 3 72 209 161 292 8.93e-21 94.7
MS.gene76156.t1 AT3G59690 37.324 142 75 3 72 209 161 292 8.93e-21 94.7
MS.gene76156.t1 AT3G15050 31.429 210 119 5 83 283 52 245 1.36e-20 90.9
MS.gene76156.t1 AT2G26410 34.054 185 103 5 79 256 146 318 5.39e-20 92.4
MS.gene76156.t1 AT2G26410 34.054 185 103 5 79 256 146 318 5.39e-20 92.4
MS.gene76156.t1 AT2G33990 31.818 176 109 3 80 255 46 210 7.10e-19 86.3
MS.gene76156.t1 AT1G18840 45.745 94 47 1 78 167 106 199 4.38e-18 86.7
MS.gene76156.t1 AT1G18840 45.745 94 47 1 78 167 106 199 4.38e-18 86.7
MS.gene76156.t1 AT1G18840 45.745 94 47 1 78 167 106 199 4.38e-18 86.7
MS.gene76156.t1 AT4G23060 58.108 74 31 0 81 154 167 240 5.31e-18 86.3
MS.gene76156.t1 AT4G29150 42.500 120 61 2 69 180 119 238 1.46e-17 84.3
MS.gene76156.t1 AT5G13460 36.552 145 71 4 75 207 103 238 4.58e-17 83.2
MS.gene76156.t1 AT5G07240 29.252 294 138 11 80 363 104 337 4.62e-17 82.8
MS.gene76156.t1 AT5G07240 28.716 296 137 11 80 363 150 383 6.07e-17 82.8
MS.gene76156.t1 AT1G51960 50.000 80 37 2 69 147 89 166 7.01e-17 81.6
MS.gene76156.t1 AT3G51380 57.576 66 28 0 79 144 35 100 9.03e-17 75.9
MS.gene76156.t1 AT1G14380 34.568 162 89 4 1 147 1 160 6.19e-16 80.5
MS.gene76156.t1 AT1G14380 34.568 162 89 4 1 147 1 160 6.19e-16 80.5
MS.gene76156.t1 AT1G14380 34.568 162 89 4 1 147 1 160 6.19e-16 80.5
MS.gene76156.t1 AT1G14380 34.568 162 89 4 1 147 1 160 7.47e-16 80.1
MS.gene76156.t1 AT1G14380 34.568 162 89 4 1 147 1 160 7.47e-16 80.1
MS.gene76156.t1 AT1G14380 34.568 162 89 4 1 147 1 160 7.47e-16 80.1
MS.gene76156.t1 AT1G14380 34.568 162 89 4 1 147 1 160 8.75e-16 79.7
MS.gene76156.t1 AT1G74690 50.000 76 38 0 78 153 110 185 2.29e-15 78.6
MS.gene76156.t1 AT5G03960 38.060 134 80 2 83 213 110 243 2.10e-14 74.7
MS.gene76156.t1 AT5G03960 38.060 134 80 2 83 213 110 243 2.10e-14 74.7
MS.gene76156.t1 AT5G03960 38.060 134 80 2 83 213 109 242 2.11e-14 74.7
MS.gene76156.t1 AT2G43680 33.099 142 88 4 66 207 310 444 2.45e-14 75.5
MS.gene76156.t1 AT2G43680 33.099 142 88 4 66 207 310 444 2.45e-14 75.5
MS.gene76156.t1 AT2G43680 33.099 142 88 4 66 207 310 444 2.45e-14 75.5
MS.gene76156.t1 AT2G43680 33.099 142 88 4 66 207 310 444 2.45e-14 75.5
MS.gene76156.t1 AT2G43680 33.099 142 88 4 66 207 311 445 2.49e-14 75.5
MS.gene76156.t1 AT1G19870 34.483 145 77 3 47 175 165 307 6.50e-14 74.3
MS.gene76156.t1 AT1G19870 38.938 113 56 2 74 175 14 124 1.13e-13 73.2
MS.gene76156.t1 AT5G62070 64.151 53 19 0 83 135 119 171 5.47e-13 70.5
MS.gene76156.t1 AT3G49380 34.454 119 66 2 27 137 52 166 1.10e-12 69.3

Find 79 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGACCCTTCAGTTAATTTA+TGG 0.180664 2.3:+3934054 MS.gene76156:CDS
CAATGGGTGTTTCTGGAAAA+TGG 0.187083 2.3:+3931773 None:intergenic
TGCTGCACTGCTTTGCCTTC+TGG 0.231347 2.3:-3933156 None:intergenic
GGCTCTCTTTCGAGCCTTCT+TGG 0.249229 2.3:+3932121 MS.gene76156:CDS
TGATAATAACTTGCCCTTAG+AGG 0.285753 2.3:-3934095 None:intergenic
CTAAAGATTTCATCCTTATC+AGG 0.290356 2.3:+3932072 MS.gene76156:CDS
TCACTCTTCCTGCTTGAGAT+TGG 0.302121 2.3:-3933678 None:intergenic
TTAACTTCATCTGCAGTTCC+AGG 0.344133 2.3:-3933366 None:intergenic
GCAAGCTCTGGTTAGGGTTC+AGG 0.355190 2.3:+3933101 MS.gene76156:CDS
ATGGGCTGCTATTCGCATTC+AGG 0.358781 2.3:+3932100 MS.gene76156:CDS
TCTGTTGCTTCTGGCATAGA+TGG 0.359986 2.3:-3933855 None:intergenic
TTATCAACTTCATGTACATC+TGG 0.371891 2.3:+3933828 MS.gene76156:CDS
CTGATAAGGATGAAATCTTT+AGG 0.373735 2.3:-3932071 None:intergenic
GCTATCATCATTCATGTATT+CGG 0.376299 2.3:-3933802 None:intergenic
AACAAGCAGCTGTTACTCTA+AGG 0.378838 2.3:+3933073 MS.gene76156:CDS
GTAATAGTACTAAGAAGAAA+TGG 0.387683 2.3:+3931925 MS.gene76156:CDS
AGGCTAAGCTCAAAACTTAC+AGG 0.390491 2.3:+3933928 MS.gene76156:CDS
GGTGTATGCAAGCTCTGGTT+AGG 0.390529 2.3:+3933094 MS.gene76156:CDS
ATCGTTGTGCAACACCTCTA+AGG 0.396953 2.3:+3934081 MS.gene76156:CDS
AGCAGCACCAGTCTTCTTCA+TGG 0.398235 2.3:-3931982 None:intergenic
CAAGGATGGTGTGAAATTCC+TGG 0.403362 2.3:+3933348 MS.gene76156:CDS
GATAATAGTATAAACAGTTC+TGG 0.410910 2.3:+3934029 MS.gene76156:CDS
TTCCTTCTTGATTGAAAAGT+AGG 0.412386 2.3:-3933711 None:intergenic
CATTGCTCTATCCCTCTTGA+TGG 0.414300 2.3:-3933418 None:intergenic
TTCCTAAGCTCTTGTTGTCA+TGG 0.417603 2.3:-3933605 None:intergenic
AGTTATTATCAAAATCGATA+TGG 0.428695 2.3:+3934107 MS.gene76156:CDS
CAGCCCATTCCTGCCTGATA+AGG 0.431812 2.3:-3932085 None:intergenic
CATCCTTATCAGGCAGGAAT+GGG 0.450099 2.3:+3932082 MS.gene76156:CDS
GTTGCTGTCGTTGTTCGAGC+TGG 0.450476 2.3:+3932047 MS.gene76156:CDS
AAGAGCTTTGAGAGCATTGA+GGG 0.454446 2.3:+3933005 MS.gene76156:CDS
CTAAGAAGAAATGGAAGCTA+TGG 0.454548 2.3:+3931934 MS.gene76156:CDS
TCATCCTTATCAGGCAGGAA+TGG 0.473411 2.3:+3932081 MS.gene76156:CDS
TTCCAATAAGAAAACCGATC+AGG 0.485410 2.3:+3931810 MS.gene76156:CDS
AGACTGCAAGCAATATTTAG+AGG 0.495590 2.3:+3933036 MS.gene76156:CDS
GAAATAGCTTGTTAGAGCGC+TGG 0.496586 2.3:+3933625 MS.gene76156:CDS
TGCAAGCAATATTTAGAGGC+AGG 0.511357 2.3:+3933040 MS.gene76156:CDS
TCTAAGGTGTATGCAAGCTC+TGG 0.513287 2.3:+3933089 MS.gene76156:CDS
AGATTTCATCCTTATCAGGC+AGG 0.519223 2.3:+3932076 MS.gene76156:CDS
GTGTATGCAAGCTCTGGTTA+GGG 0.521748 2.3:+3933095 MS.gene76156:CDS
GTTATTATTATCAGCAAGGA+TGG 0.522365 2.3:+3933334 MS.gene76156:intron
GGCAAGAAGGTGCCATCAAG+AGG 0.522656 2.3:+3933406 MS.gene76156:CDS
TGATTGTAAATTTACCTGAT+CGG 0.522844 2.3:-3931824 None:intergenic
TCTTCAACCATGAAGAAGAC+TGG 0.527272 2.3:+3931975 MS.gene76156:CDS
TGGCAAACAAACCATGTCCA+TGG 0.529332 2.3:+3933649 MS.gene76156:CDS
GGCAGCAGCAGTATCGGTAA+TGG 0.530108 2.3:-3932024 None:intergenic
CCCTTCAGTTAATTTATGGA+AGG 0.533849 2.3:+3934058 MS.gene76156:CDS
GAGCTTAAAGTCGAAGATGA+TGG 0.534371 2.3:-3933780 None:intergenic
TTCAGGCACGTGTGAGAGCT+AGG 0.537833 2.3:+3933118 MS.gene76156:CDS
AACCATGACAACAAGAGCTT+AGG 0.537945 2.3:+3933603 MS.gene76156:CDS
AGCAACGGCAGCAGCAGTAT+CGG 0.538131 2.3:-3932030 None:intergenic
AAGCTAAGAATGAGGCAAGA+AGG 0.538231 2.3:+3933393 MS.gene76156:CDS
TCAAATTCATGTAGCTAGGT+AGG 0.539947 2.3:-3933893 None:intergenic
TCCTTCCATAAATTAACTGA+AGG 0.545088 2.3:-3934059 None:intergenic
ATTGGACTCTCCCATGGACA+TGG 0.548204 2.3:-3933660 None:intergenic
CAGAATCAGCATGGTTGCGA+TGG 0.549893 2.3:-3933191 None:intergenic
ATTATCAAAATCGATATGGC+AGG 0.555854 2.3:+3934111 MS.gene76156:CDS
TCGTTGTGCAACACCTCTAA+GGG 0.556401 2.3:+3934082 MS.gene76156:CDS
CTTGAGATTGGACTCTCCCA+TGG 0.558321 2.3:-3933666 None:intergenic
AAAAGAAATAGTTACCAAGA+AGG 0.563052 2.3:-3932135 None:intergenic
GAGTTCGAAGAGACTCGTAA+TGG 0.563998 2.3:+3933962 MS.gene76156:CDS
TTTGAGAGCATTGAGGGCAG+TGG 0.564489 2.3:+3933011 MS.gene76156:CDS
GAAGAGCTTTGAGAGCATTG+AGG 0.569321 2.3:+3933004 MS.gene76156:CDS
CCTTCCATAAATTAACTGAA+GGG 0.587260 2.3:-3934058 None:intergenic
TCTGTCAAATTCATGTAGCT+AGG 0.592487 2.3:-3933897 None:intergenic
GAATTTGACAGAATCAACTA+AGG 0.594832 2.3:+3933908 MS.gene76156:CDS
GGGAGAGTCCAATCTCAAGC+AGG 0.595864 2.3:+3933670 MS.gene76156:CDS
GGCAAACAAACCATGTCCAT+GGG 0.598464 2.3:+3933650 MS.gene76156:CDS
TTAAAGCAAAGCTAAGAATG+AGG 0.599503 2.3:+3933385 MS.gene76156:CDS
ATCTATGCCAGAAGCAACAG+AGG 0.608408 2.3:+3933857 MS.gene76156:CDS
TGATTCTGTTAAGCTTGTAG+AGG 0.611493 2.3:+3933206 MS.gene76156:CDS
AGCTCGAACAACGACAGCAA+CGG 0.615911 2.3:-3932045 None:intergenic
CATCAAGAGGGATAGAGCAA+TGG 0.627816 2.3:+3933419 MS.gene76156:CDS
TAGCTTGTTAGAGCGCTGGA+TGG 0.632949 2.3:+3933629 MS.gene76156:CDS
TCAACACTTAATTACCTGTG+TGG 0.634403 2.3:-3933454 None:intergenic
GCAAGAAGGTGCCATCAAGA+GGG 0.646053 2.3:+3933407 MS.gene76156:CDS
GTGTGAGAGCTAGGAATGTG+AGG 0.652883 2.3:+3933127 MS.gene76156:CDS
CAAGCTTAACAGAATCAGCA+TGG 0.655634 2.3:-3933200 None:intergenic
GGCATACTCTCTATCCACAC+AGG 0.672618 2.3:+3933440 MS.gene76156:CDS
AATGTGAGGAAATCTCCAGA+AGG 0.698682 2.3:+3933141 MS.gene76156:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTCAAAATATGATAATATA+AGG - chr2.3:3932605-3932624 None:intergenic 10.0%
!! AAAAGAGAAAGAAAAAATAA+AGG - chr2.3:3932709-3932728 None:intergenic 15.0%
!! AAAGAGAAAGAAAAAATAAA+GGG - chr2.3:3932708-3932727 None:intergenic 15.0%
!! ATTACATAATATTCTCTAAA+TGG + chr2.3:3933257-3933276 MS.gene76156:intron 15.0%
!! TAAAAAGTTGAATTTCAATT+AGG - chr2.3:3932656-3932675 None:intergenic 15.0%
!! TAAAACAACAACAAAAAAAA+GGG - chr2.3:3931866-3931885 None:intergenic 15.0%
!! TATAGTATATGCTAATATAA+GGG + chr2.3:3933299-3933318 MS.gene76156:intron 15.0%
!! TTAAAACAACAACAAAAAAA+AGG - chr2.3:3931867-3931886 None:intergenic 15.0%
!! TTATAGTATATGCTAATATA+AGG + chr2.3:3933298-3933317 MS.gene76156:intron 15.0%
!!! TGTATTTTAAAAAGAAATAG+AGG - chr2.3:3932974-3932993 None:intergenic 15.0%
!!! TTAATTAGTTTATCTTATGA+TGG + chr2.3:3932579-3932598 MS.gene76156:intron 15.0%
!!! TTATTTTTTCTTTCTCTTTT+TGG + chr2.3:3932709-3932728 MS.gene76156:intron 15.0%
!!! TTCTTTTTAAAATACATTGA+AGG + chr2.3:3932978-3932997 MS.gene76156:intron 15.0%
!! AAGAGAAAGAAAAAATAAAG+GGG - chr2.3:3932707-3932726 None:intergenic 20.0%
!! AGTTATTATCAAAATCGATA+TGG + chr2.3:3934107-3934126 MS.gene76156:CDS 20.0%
!! TGCAATAAATCCTAAAAAAA+TGG - chr2.3:3932851-3932870 None:intergenic 20.0%
! AAAAAAAAAGTAACCAAGAG+TGG - chr2.3:3932884-3932903 None:intergenic 25.0%
! AAAAGAAATAGTTACCAAGA+AGG - chr2.3:3932138-3932157 None:intergenic 25.0%
! AAAAGCAAGCAAAGATATAT+TGG - chr2.3:3933511-3933530 None:intergenic 25.0%
! AAGTTTACATGACTTAAAAG+AGG - chr2.3:3932363-3932382 None:intergenic 25.0%
! GATAATAGTATAAACAGTTC+TGG + chr2.3:3934029-3934048 MS.gene76156:CDS 25.0%
! GTAATAGTACTAAGAAGAAA+TGG + chr2.3:3931925-3931944 MS.gene76156:CDS 25.0%
! GTTGAATTTCAATTAGGTAA+AGG - chr2.3:3932650-3932669 None:intergenic 25.0%
! TGATTGTAAATTTACCTGAT+CGG - chr2.3:3931827-3931846 None:intergenic 25.0%
! TTCAAATCATGAAAACACAA+AGG - chr2.3:3932318-3932337 None:intergenic 25.0%
!! ACAAGTATAACTTTTCTGAA+TGG + chr2.3:3934005-3934024 MS.gene76156:CDS 25.0%
!! ATTTGCTAGTGTTTATAACT+CGG - chr2.3:3932440-3932459 None:intergenic 25.0%
!! TTTCTTTCTCTTTTTGGATT+GGG + chr2.3:3932715-3932734 MS.gene76156:intron 25.0%
!! TTTTCTTTCTCTTTTTGGAT+TGG + chr2.3:3932714-3932733 MS.gene76156:intron 25.0%
!!! AATGGTTATTTTGAGTTACA+AGG + chr2.3:3933275-3933294 MS.gene76156:intron 25.0%
!!! TAAACACTGTCCATTTTTTT+AGG + chr2.3:3932838-3932857 MS.gene76156:intron 25.0%
AAATCATTCAACCCCAATAA+AGG - chr2.3:3932400-3932419 None:intergenic 30.0%
AGAAAGAAAAAATAAAGGGG+AGG - chr2.3:3932704-3932723 None:intergenic 30.0%
ATTATCAAAATCGATATGGC+AGG + chr2.3:3934111-3934130 MS.gene76156:CDS 30.0%
CAATTATGGATCAGAATTCA+GGG + chr2.3:3932227-3932246 MS.gene76156:intron 30.0%
CCATGTTATTATTATCAGCA+AGG + chr2.3:3933330-3933349 MS.gene76156:intron 30.0%
CCTTCCATAAATTAACTGAA+GGG - chr2.3:3934061-3934080 None:intergenic 30.0%
CCTTGCTGATAATAATAACA+TGG - chr2.3:3933333-3933352 None:intergenic 30.0%
CTAAAGATTTCATCCTTATC+AGG + chr2.3:3932072-3932091 MS.gene76156:CDS 30.0%
CTGATAAGGATGAAATCTTT+AGG - chr2.3:3932074-3932093 None:intergenic 30.0%
GAATTTGACAGAATCAACTA+AGG + chr2.3:3933908-3933927 MS.gene76156:CDS 30.0%
GCTATCATCATTCATGTATT+CGG - chr2.3:3933805-3933824 None:intergenic 30.0%
GGATTTGGGAGTTTAAAAAA+CGG + chr2.3:3932291-3932310 MS.gene76156:intron 30.0%
GTTATTATTATCAGCAAGGA+TGG + chr2.3:3933334-3933353 MS.gene76156:intron 30.0%
GTTGAATGATTTACGTAGTT+AGG + chr2.3:3932408-3932427 MS.gene76156:intron 30.0%
TAAAACAATGTGAAGAAGAG+TGG - chr2.3:3932489-3932508 None:intergenic 30.0%
TACAAGATCTACCAATAGTT+GGG + chr2.3:3932270-3932289 MS.gene76156:intron 30.0%
TCCTTCCATAAATTAACTGA+AGG - chr2.3:3934062-3934081 None:intergenic 30.0%
TGAAATTCCAAGTCCAATTA+TGG + chr2.3:3932213-3932232 MS.gene76156:intron 30.0%
TTAAAGCAAAGCTAAGAATG+AGG + chr2.3:3933385-3933404 MS.gene76156:CDS 30.0%
TTACAAGATCTACCAATAGT+TGG + chr2.3:3932269-3932288 MS.gene76156:intron 30.0%
TTATCAACTTCATGTACATC+TGG + chr2.3:3933828-3933847 MS.gene76156:CDS 30.0%
TTCCTTCTTGATTGAAAAGT+AGG - chr2.3:3933714-3933733 None:intergenic 30.0%
TTTATGCTGAATGATAGACT+CGG - chr2.3:3932822-3932841 None:intergenic 30.0%
! ACTTTTCAATCAAGAAGGAA+TGG + chr2.3:3933714-3933733 MS.gene76156:CDS 30.0%
! AGGATTTTACAGTCATCTTA+AGG - chr2.3:3932684-3932703 None:intergenic 30.0%
! AGTAGTTAGGTTCTTTTGTA+TGG + chr2.3:3932756-3932775 MS.gene76156:intron 30.0%
! AGTGTTAATGTTGTTTGTTC+AGG + chr2.3:3931893-3931912 MS.gene76156:intron 30.0%
! ATTTTACAGTCATCTTAAGG+AGG - chr2.3:3932681-3932700 None:intergenic 30.0%
! TTCTTTCTCTTTTTGGATTG+GGG + chr2.3:3932716-3932735 MS.gene76156:intron 30.0%
!! TTCCTACTTTTCAATCAAGA+AGG + chr2.3:3933709-3933728 MS.gene76156:CDS 30.0%
!!! GATTTTGTGGATTTTGAGTT+TGG - chr2.3:3933558-3933577 None:intergenic 30.0%
!!! TCCTCCTTTTTTCCTTTATT+GGG + chr2.3:3932385-3932404 MS.gene76156:intron 30.0%
!!! TTCCTCCTTTTTTCCTTTAT+TGG + chr2.3:3932384-3932403 MS.gene76156:intron 30.0%
AGACTGCAAGCAATATTTAG+AGG + chr2.3:3933036-3933055 MS.gene76156:CDS 35.0%
ATCTACCAATAGTTGGGATT+TGG + chr2.3:3932276-3932295 MS.gene76156:intron 35.0%
CAACCCCAATAAAGGAAAAA+AGG - chr2.3:3932392-3932411 None:intergenic 35.0%
CCAATTATGGATCAGAATTC+AGG + chr2.3:3932226-3932245 MS.gene76156:intron 35.0%
CCACAGCAAAAATCATAGAA+AGG - chr2.3:3932161-3932180 None:intergenic 35.0%
CCCTTCAGTTAATTTATGGA+AGG + chr2.3:3934058-3934077 MS.gene76156:CDS 35.0%
CCTGAATTCTGATCCATAAT+TGG - chr2.3:3932229-3932248 None:intergenic 35.0%
CTAAGAAGAAATGGAAGCTA+TGG + chr2.3:3931934-3931953 MS.gene76156:CDS 35.0%
CTGACCCTTCAGTTAATTTA+TGG + chr2.3:3934054-3934073 MS.gene76156:CDS 35.0%
TCAAATTCATGTAGCTAGGT+AGG - chr2.3:3933896-3933915 None:intergenic 35.0%
TCAACACTTAATTACCTGTG+TGG - chr2.3:3933457-3933476 None:intergenic 35.0%
TCTACCAATAGTTGGGATTT+GGG + chr2.3:3932277-3932296 MS.gene76156:intron 35.0%
TCTGTCAAATTCATGTAGCT+AGG - chr2.3:3933900-3933919 None:intergenic 35.0%
TGATTCTGTTAAGCTTGTAG+AGG + chr2.3:3933206-3933225 MS.gene76156:CDS 35.0%
TTCCAATAAGAAAACCGATC+AGG + chr2.3:3931810-3931829 MS.gene76156:CDS 35.0%
TTCTGATCCATAATTGGACT+TGG - chr2.3:3932223-3932242 None:intergenic 35.0%
! CCTTTCTATGATTTTTGCTG+TGG + chr2.3:3932158-3932177 MS.gene76156:intron 35.0%
! TGATAATAACTTGCCCTTAG+AGG - chr2.3:3934098-3934117 None:intergenic 35.0%
!! TACCTGATCGGTTTTCTTAT+TGG - chr2.3:3931815-3931834 None:intergenic 35.0%
!! TCTTTCTCTTTTTGGATTGG+GGG + chr2.3:3932717-3932736 MS.gene76156:intron 35.0%
!!! CCTCCTTTTTTCCTTTATTG+GGG + chr2.3:3932386-3932405 MS.gene76156:intron 35.0%
!!! GAAAAGAGACACTGATTTTG+TGG - chr2.3:3933571-3933590 None:intergenic 35.0%
AACAAGCAGCTGTTACTCTA+AGG + chr2.3:3933073-3933092 MS.gene76156:CDS 40.0%
AACCATGACAACAAGAGCTT+AGG + chr2.3:3933603-3933622 MS.gene76156:CDS 40.0%
AACTCCCAAATCCCAACTAT+TGG - chr2.3:3932284-3932303 None:intergenic 40.0%
AAGCTAAGAATGAGGCAAGA+AGG + chr2.3:3933393-3933412 MS.gene76156:CDS 40.0%
AATGTGAGGAAATCTCCAGA+AGG + chr2.3:3933141-3933160 MS.gene76156:CDS 40.0%
AGATTTCATCCTTATCAGGC+AGG + chr2.3:3932076-3932095 MS.gene76156:CDS 40.0%
AGGCTAAGCTCAAAACTTAC+AGG + chr2.3:3933928-3933947 MS.gene76156:CDS 40.0%
CAAGCTTAACAGAATCAGCA+TGG - chr2.3:3933203-3933222 None:intergenic 40.0%
CCCCAATAAAGGAAAAAAGG+AGG - chr2.3:3932389-3932408 None:intergenic 40.0%
GAGCTTAAAGTCGAAGATGA+TGG - chr2.3:3933783-3933802 None:intergenic 40.0%
GGTCAATGACTTCAGTAGTT+AGG + chr2.3:3932743-3932762 MS.gene76156:intron 40.0%
TCTTCAACCATGAAGAAGAC+TGG + chr2.3:3931975-3931994 MS.gene76156:CDS 40.0%
TGCAAGCAATATTTAGAGGC+AGG + chr2.3:3933040-3933059 MS.gene76156:CDS 40.0%
TGGATCAGAATTCAGGGATT+AGG + chr2.3:3932233-3932252 MS.gene76156:intron 40.0%
TTAACTTCATCTGCAGTTCC+AGG - chr2.3:3933369-3933388 None:intergenic 40.0%
! TAGGTTCTTTTGTATGGCTG+TGG + chr2.3:3932762-3932781 MS.gene76156:intron 40.0%
! TTCCTAAGCTCTTGTTGTCA+TGG - chr2.3:3933608-3933627 None:intergenic 40.0%
!! AAGAGCTTTGAGAGCATTGA+GGG + chr2.3:3933005-3933024 MS.gene76156:CDS 40.0%
!! GTGGTGTTACTAATCTGTCA+GGG + chr2.3:3932781-3932800 MS.gene76156:intron 40.0%
!! TCTTTTTCCTCTGTTGCTTC+TGG - chr2.3:3933867-3933886 None:intergenic 40.0%
!! TGGTGTTACTAATCTGTCAG+GGG + chr2.3:3932782-3932801 MS.gene76156:intron 40.0%
!! TGTGGTGTTACTAATCTGTC+AGG + chr2.3:3932780-3932799 MS.gene76156:intron 40.0%
ATCGTTGTGCAACACCTCTA+AGG + chr2.3:3934081-3934100 MS.gene76156:CDS 45.0%
ATCTATGCCAGAAGCAACAG+AGG + chr2.3:3933857-3933876 MS.gene76156:CDS 45.0%
CAAGGATGGTGTGAAATTCC+TGG + chr2.3:3933348-3933367 MS.gene76156:CDS 45.0%
CATCAAGAGGGATAGAGCAA+TGG + chr2.3:3933419-3933438 MS.gene76156:CDS 45.0%
CATCCTTATCAGGCAGGAAT+GGG + chr2.3:3932082-3932101 MS.gene76156:CDS 45.0%
CATTGCTCTATCCCTCTTGA+TGG - chr2.3:3933421-3933440 None:intergenic 45.0%
GAGTTCGAAGAGACTCGTAA+TGG + chr2.3:3933962-3933981 MS.gene76156:CDS 45.0%
GCAGAAATATCAGCCACTCT+TGG + chr2.3:3932868-3932887 MS.gene76156:intron 45.0%
GGCAAACAAACCATGTCCAT+GGG + chr2.3:3933650-3933669 MS.gene76156:CDS 45.0%
TCACTCTTCCTGCTTGAGAT+TGG - chr2.3:3933681-3933700 None:intergenic 45.0%
TCATCCTTATCAGGCAGGAA+TGG + chr2.3:3932081-3932100 MS.gene76156:CDS 45.0%
TCGTTGTGCAACACCTCTAA+GGG + chr2.3:3934082-3934101 MS.gene76156:CDS 45.0%
TGGCAAACAAACCATGTCCA+TGG + chr2.3:3933649-3933668 MS.gene76156:CDS 45.0%
! GTGTATGCAAGCTCTGGTTA+GGG + chr2.3:3933095-3933114 MS.gene76156:CDS 45.0%
! TCTAAGGTGTATGCAAGCTC+TGG + chr2.3:3933089-3933108 MS.gene76156:CDS 45.0%
! TCTGTTGCTTCTGGCATAGA+TGG - chr2.3:3933858-3933877 None:intergenic 45.0%
!! CTCTTTTTGGATTGGGGGAT+GGG + chr2.3:3932722-3932741 MS.gene76156:intron 45.0%
!! GAAATAGCTTGTTAGAGCGC+TGG + chr2.3:3933625-3933644 MS.gene76156:CDS 45.0%
!! GAAGAGCTTTGAGAGCATTG+AGG + chr2.3:3933004-3933023 MS.gene76156:CDS 45.0%
!! TCTCTTTTTGGATTGGGGGA+TGG + chr2.3:3932721-3932740 MS.gene76156:intron 45.0%
AGCAGCACCAGTCTTCTTCA+TGG - chr2.3:3931985-3932004 None:intergenic 50.0%
AGCTCGAACAACGACAGCAA+CGG - chr2.3:3932048-3932067 None:intergenic 50.0%
ATTGGACTCTCCCATGGACA+TGG - chr2.3:3933663-3933682 None:intergenic 50.0%
CAGAATCAGCATGGTTGCGA+TGG - chr2.3:3933194-3933213 None:intergenic 50.0%
CTTGAGATTGGACTCTCCCA+TGG - chr2.3:3933669-3933688 None:intergenic 50.0%
GGCATACTCTCTATCCACAC+AGG + chr2.3:3933440-3933459 MS.gene76156:CDS 50.0%
GTGTGAGAGCTAGGAATGTG+AGG + chr2.3:3933127-3933146 MS.gene76156:CDS 50.0%
! ATGGGCTGCTATTCGCATTC+AGG + chr2.3:3932100-3932119 MS.gene76156:CDS 50.0%
! GGTGTATGCAAGCTCTGGTT+AGG + chr2.3:3933094-3933113 MS.gene76156:CDS 50.0%
!! GCAAGAAGGTGCCATCAAGA+GGG + chr2.3:3933407-3933426 MS.gene76156:CDS 50.0%
!! TAGCTTGTTAGAGCGCTGGA+TGG + chr2.3:3933629-3933648 MS.gene76156:CDS 50.0%
!! TTTGAGAGCATTGAGGGCAG+TGG + chr2.3:3933011-3933030 MS.gene76156:CDS 50.0%
AGCAACGGCAGCAGCAGTAT+CGG - chr2.3:3932033-3932052 None:intergenic 55.0%
CAGCCCATTCCTGCCTGATA+AGG - chr2.3:3932088-3932107 None:intergenic 55.0%
GGCTCTCTTTCGAGCCTTCT+TGG + chr2.3:3932121-3932140 MS.gene76156:CDS 55.0%
GGGAGAGTCCAATCTCAAGC+AGG + chr2.3:3933670-3933689 MS.gene76156:CDS 55.0%
GTTGCTGTCGTTGTTCGAGC+TGG + chr2.3:3932047-3932066 MS.gene76156:CDS 55.0%
TGCTGCACTGCTTTGCCTTC+TGG - chr2.3:3933159-3933178 None:intergenic 55.0%
TTCAGGCACGTGTGAGAGCT+AGG + chr2.3:3933118-3933137 MS.gene76156:CDS 55.0%
! GCAAGCTCTGGTTAGGGTTC+AGG + chr2.3:3933101-3933120 MS.gene76156:CDS 55.0%
!! GGCAAGAAGGTGCCATCAAG+AGG + chr2.3:3933406-3933425 MS.gene76156:CDS 55.0%
!! GGCAGCAGCAGTATCGGTAA+TGG - chr2.3:3932027-3932046 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 3931775 3934139 3931775 ID=MS.gene76156
chr2.3 mRNA 3931775 3934139 3931775 ID=MS.gene76156.t1;Parent=MS.gene76156
chr2.3 exon 3931775 3931831 3931775 ID=MS.gene76156.t1.exon1;Parent=MS.gene76156.t1
chr2.3 CDS 3931775 3931831 3931775 ID=cds.MS.gene76156.t1;Parent=MS.gene76156.t1
chr2.3 exon 3931915 3932142 3931915 ID=MS.gene76156.t1.exon2;Parent=MS.gene76156.t1
chr2.3 CDS 3931915 3932142 3931915 ID=cds.MS.gene76156.t1;Parent=MS.gene76156.t1
chr2.3 exon 3933000 3933227 3933000 ID=MS.gene76156.t1.exon3;Parent=MS.gene76156.t1
chr2.3 CDS 3933000 3933227 3933000 ID=cds.MS.gene76156.t1;Parent=MS.gene76156.t1
chr2.3 exon 3933348 3933461 3933348 ID=MS.gene76156.t1.exon4;Parent=MS.gene76156.t1
chr2.3 CDS 3933348 3933461 3933348 ID=cds.MS.gene76156.t1;Parent=MS.gene76156.t1
chr2.3 exon 3933537 3934139 3933537 ID=MS.gene76156.t1.exon5;Parent=MS.gene76156.t1
chr2.3 CDS 3933537 3934139 3933537 ID=cds.MS.gene76156.t1;Parent=MS.gene76156.t1
Gene Sequence

>MS.gene76156

ATGGGTGTTTCTGGAAAATGGTTTAAATCACTTCTTTCCAATAAGAAAACCGATCAGGTAAATTTACAATCACTTCTTTTATTTTTTTCCCTTTTTTTTGTTGTTGTTTTAATAATTTAGTGTTAATGTTGTTTGTTCAGGAGAAGAAGTGTAATAGTACTAAGAAGAAATGGAAGCTATGGAAAACTTCTTCTTCTTCTTCTTCAACCATGAAGAAGACTGGTGCTGCTGCTGCTGTGTCTGATTCTTCCATTACCGATACTGCTGCTGCCGTTGCTGTCGTTGTTCGAGCTGGACCTAAAGATTTCATCCTTATCAGGCAGGAATGGGCTGCTATTCGCATTCAGGCTCTCTTTCGAGCCTTCTTGGTAACTATTTCTTTTCCTTTCTATGATTTTTGCTGTGGCTTTTAATTGTTCATGTCATAGTGTGCTAGTTTGAAATTCCAAGTCCAATTATGGATCAGAATTCAGGGATTAGGATCTATTAAGTTTTTACAAGATCTACCAATAGTTGGGATTTGGGAGTTTAAAAAACGGACCTTTGTGTTTTCATGATTTGAATTTGAATTTTCAACTTTTTAGTCCTCTTTTAAGTCATGTAAACTTTTTCCTCCTTTTTTCCTTTATTGGGGTTGAATGATTTACGTAGTTAGGTAATAACCGAGTTATAAACACTAGCAAATGTTACGAAAAATTGCAGAAATATTAGCCACTCTTCTTCACATTGTTTTAAGAGAATTTGATCAGTATATTTTGCATATGTTGCTTGTGCTTAGATTTTTCTATGCTAATGTTCTTCATGTTAATTAGTTTATCTTATGATGGCCTTATATTATCATATTTTGAATTTTGAAATCAGCTCGCAGCTCGCCTTTACCTAATTGAAATTCAACTTTTTAGTCCTCCTTAAGATGACTGTAAAATCCTCCCCTTTATTTTTTCTTTCTCTTTTTGGATTGGGGGATGGGTCAATGACTTCAGTAGTTAGGTTCTTTTGTATGGCTGTGGTGTTACTAATCTGTCAGGGGAATATGATGCATAACCGAGTCTATCATTCAGCATAAACACTGTCCATTTTTTTAGGATTTATTGCAGAAATATCAGCCACTCTTGGTTACTTTTTTTTTTTTTTCTTCTTCTTTATCTATGTTGATACTCTTCTTTCATTGATTTAGTTATTTTATATTGATCTTACCTCTATTTCTTTTTAAAATACATTGAAGGCTAGAAGAGCTTTGAGAGCATTGAGGGCAGTGGTGAGACTGCAAGCAATATTTAGAGGCAGGCAAGTTCGAAAACAAGCAGCTGTTACTCTAAGGTGTATGCAAGCTCTGGTTAGGGTTCAGGCACGTGTGAGAGCTAGGAATGTGAGGAAATCTCCAGAAGGCAAAGCAGTGCAGCAGCTATTAGATGACCATCGCAACCATGCTGATTCTGTTAAGCTTGTAGAGGTAGCAAATTAATTTGTTTCAAGATAATAATTACATAATATTCTCTAAATGGTTATTTTGAGTTACAAGGTTATAGTATATGCTAATATAAGGGATAATTTGCCATGTTATTATTATCAGCAAGGATGGTGTGAAATTCCTGGAACTGCAGATGAAGTTAAAGCAAAGCTAAGAATGAGGCAAGAAGGTGCCATCAAGAGGGATAGAGCAATGGCATACTCTCTATCCACACAGGTAATTAAGTGTTGACAATATCTATTCATTTAGTTTAGTTTCAATTCCAATATATCTTTGCTTGCTTTTTAACAGTCAAGAATAAGTGCTAGTCCAAACTCAAAATCCACAAAATCAGTGTCTCTTTTCAAGCATCATCACAACCATGACAACAAGAGCTTAGGAAATAGCTTGTTAGAGCGCTGGATGGCAAACAAACCATGTCCATGGGAGAGTCCAATCTCAAGCAGGAAGAGTGAAGAACTTGTTCCTACTTTTCAATCAAGAAGGAATGGTGTGACAACAAGAATTTCAGCTCTTAAAATCTGTCAACAAACTCCATCATCTTCGACTTTAAGCTCCGAATACATGAATGATGATAGCATTTTATCAACTTCATGTACATCTGGATCTCCATCTATGCCAGAAGCAACAGAGGAAAAAGATGCTCACCTACCTAGCTACATGAATTTGACAGAATCAACTAAGGCTAAGCTCAAAACTTACAGGTCTTCTTCGCAGAGTTCGAAGAGACTCGTAATGGATGATTGTATGTCTCATAACACAAGTATAACTTTTCTGAATGGAGATAATAGTATAAACAGTTCTGGTTCTGACCCTTCAGTTAATTTATGGAAGGATCGTTGTGCAACACCTCTAAGGGCAAGTTATTATCAAAATCGATATGGCAGGAGATAG
Protein sequence

>MS.gene76156.t1

MGVSGKWFKSLLSNKKTDQEKKCNSTKKKWKLWKTSSSSSSTMKKTGAAAAVSDSSITDTAAAVAVVVRAGPKDFILIRQEWAAIRIQALFRAFLARRALRALRAVVRLQAIFRGRQVRKQAAVTLRCMQALVRVQARVRARNVRKSPEGKAVQQLLDDHRNHADSVKLVEQGWCEIPGTADEVKAKLRMRQEGAIKRDRAMAYSLSTQSRISASPNSKSTKSVSLFKHHHNHDNKSLGNSLLERWMANKPCPWESPISSRKSEELVPTFQSRRNGVTTRISALKICQQTPSSSTLSSEYMNDDSILSTSCTSGSPSMPEATEEKDAHLPSYMNLTESTKAKLKTYRSSSQSSKRLVMDDCMSHNTSITFLNGDNSINSSGSDPSVNLWKDRCATPLRASYYQNRYGRR