AlfalfaGEDB | MS.gene76497

M. sativa cultivar XinJiangDaYe / MS.gene76497

KEGG: K10418
GO: GO:0005575 GO:0005875 GO:0007017 GO:0008150 GO:0009987 GO:0030286 GO:0032991 GO:1902494
PFAM: PF01221.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76497.t1	XP_003597748.1	100	91	0	0	1	91	1	91	2.00E-44	188

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76497.t1	Q94748	47.2	89	45	1	1	89	1	87	4.1e-14	78.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76497.t1	G7II18	100.0	91	0	0	1	91	1	91	1.4e-44	188.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050303	MS.gene76497	0.809786	1.59E-50	-1.69E-46
MS.gene050330	MS.gene76497	-0.833813	4.30E-56	-1.69E-46
MS.gene050807	MS.gene76497	0.806198	9.24E-50	-1.69E-46
MS.gene052176	MS.gene76497	0.836068	1.16E-56	-1.69E-46
MS.gene052302	MS.gene76497	0.845436	4.07E-59	-1.69E-46
MS.gene052358	MS.gene76497	-0.816083	6.64E-52	-1.69E-46
MS.gene052376	MS.gene76497	0.854098	1.54E-61	-1.69E-46
MS.gene053054	MS.gene76497	-0.824437	8.08E-54	-1.69E-46
MS.gene054942	MS.gene76497	0.805861	1.09E-49	-1.69E-46
MS.gene056091	MS.gene76497	0.800312	1.53E-48	-1.69E-46
MS.gene057060	MS.gene76497	0.822028	2.95E-53	-1.69E-46
MS.gene057967	MS.gene76497	0.802915	4.47E-49	-1.69E-46
MS.gene05954	MS.gene76497	0.816439	5.53E-52	-1.69E-46
MS.gene060572	MS.gene76497	0.826891	2.11E-54	-1.69E-46
MS.gene061040	MS.gene76497	-0.811238	7.74E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76497.t1	MTR_2g101850	100.000	91	0	0	1	91	1	91	7.67e-65	190
MS.gene76497.t1	MTR_6g087770	75.556	90	22	0	1	90	1	90	6.48e-50	152
MS.gene76497.t1	MTR_8g464690	54.444	90	41	0	1	90	1	90	6.83e-29	100
MS.gene76497.t1	MTR_4g114060	49.367	79	40	0	11	89	159	237	5.05e-22	86.3
MS.gene76497.t1	MTR_4g107350	46.067	89	46	2	2	89	213	300	1.20e-20	83.6
MS.gene76497.t1	MTR_8g104900	41.667	84	47	1	8	89	41	124	2.57e-18	73.9
MS.gene76497.t1	MTR_1g031880	41.250	80	45	1	12	89	59	138	6.76e-18	73.2
MS.gene76497.t1	MTR_4g120150	40.698	86	49	1	5	90	46	129	3.30e-17	71.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76497.t1	AT1G52245	84.444	90	14	0	1	90	1	90	2.00e-55	166
MS.gene76497.t1	AT3G16120	78.889	90	19	0	1	90	1	90	4.72e-50	153
MS.gene76497.t1	AT3G16120	78.889	90	19	0	1	90	1	90	4.72e-50	153
MS.gene76497.t1	AT4G27360	54.444	90	41	0	1	90	1	90	1.35e-30	104
MS.gene76497.t1	AT5G20110	50.000	78	39	0	12	89	123	200	4.26e-20	80.5
MS.gene76497.t1	AT4G15930	42.353	85	47	1	5	89	39	121	1.04e-18	74.7
MS.gene76497.t1	AT1G23220	38.636	88	53	1	3	89	33	120	3.39e-18	73.6
MS.gene76497.t1	AT4G15930	44.262	61	32	1	5	65	39	97	6.59e-11	54.3

Find 12 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCAAACTCCTTTGAAAATA+AGG	0.252205	2.3:-2332339	None:intergenic
TGTGTGGTAGGTTCAAGTTT+TGG	0.339581	2.3:+2332256	MS.gene76497:CDS
GCTTGAATCTGCATCTTAGT+TGG	0.432610	2.3:-2331942	None:intergenic
TAGGAGTTTGATGCAAGATA+TGG	0.489901	2.3:+2332220	MS.gene76497:intron
GGTGGATGGCAATGTGTGGT+AGG	0.511909	2.3:+2332244	MS.gene76497:CDS
TAAGATGCAGATTCAAGCCA+TGG	0.595332	2.3:+2331947	MS.gene76497:CDS
GTAACTTCAAAGATGTTGGA+AGG	0.606545	2.3:+2331891	None:intergenic
AGCTTCAGAAGCATAAGCCA+TGG	0.620640	2.3:-2331964	None:intergenic
GAGTTTGATGCAAGATATGG+TGG	0.629614	2.3:+2332223	MS.gene76497:CDS
TTTGATGCAAGATATGGTGG+TGG	0.642657	2.3:+2332226	MS.gene76497:CDS
TGGTGGTGGATGGCAATGTG+TGG	0.654380	2.3:+2332240	MS.gene76497:CDS
ATGCAAGATATGGTGGTGGA+TGG	0.656816	2.3:+2332230	MS.gene76497:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAAATGTAACCTTTTTTATG+TGG	-	chr2.3:2332031-2332050	None:intergenic	20.0%
!!!	AAATGTAACCTTTTTTATGT+GGG	-	chr2.3:2332030-2332049	None:intergenic	20.0%
!!	TTTTCTTTGAATGTCATGTT+AGG	+	chr2.3:2332201-2332220	MS.gene76497:intron	25.0%
!!!	CTCAATTTCCTTATTTTCAA+AGG	+	chr2.3:2332331-2332350	MS.gene76497:CDS	25.0%
!!!	TGTTTTTTCACTCATTCTAA+GGG	+	chr2.3:2332280-2332299	MS.gene76497:CDS	25.0%
!!!	GTGTTTTTTCACTCATTCTA+AGG	+	chr2.3:2332279-2332298	MS.gene76497:CDS	30.0%
	TAGGAGTTTGATGCAAGATA+TGG	+	chr2.3:2332220-2332239	MS.gene76497:intron	35.0%
	CATTGCTGCCCACATAAAAA+AGG	+	chr2.3:2332019-2332038	MS.gene76497:CDS	40.0%
	GAGTTTGATGCAAGATATGG+TGG	+	chr2.3:2332223-2332242	MS.gene76497:CDS	40.0%
	GCTTGAATCTGCATCTTAGT+TGG	-	chr2.3:2331945-2331964	None:intergenic	40.0%
	TAAGATGCAGATTCAAGCCA+TGG	+	chr2.3:2331947-2331966	MS.gene76497:CDS	40.0%
	TTTGATGCAAGATATGGTGG+TGG	+	chr2.3:2332226-2332245	MS.gene76497:CDS	40.0%
!!	GTGTGGTAGGTTCAAGTTTT+GGG	+	chr2.3:2332257-2332276	MS.gene76497:CDS	40.0%
!!	TGTGTGGTAGGTTCAAGTTT+TGG	+	chr2.3:2332256-2332275	MS.gene76497:CDS	40.0%
!!!	CTTTTTTATGTGGGCAGCAA+TGG	-	chr2.3:2332021-2332040	None:intergenic	40.0%
!	AGCTTCAGAAGCATAAGCCA+TGG	-	chr2.3:2331967-2331986	None:intergenic	45.0%
!!	ATGCAAGATATGGTGGTGGA+TGG	+	chr2.3:2332230-2332249	MS.gene76497:CDS	45.0%
	GGTGGATGGCAATGTGTGGT+AGG	+	chr2.3:2332244-2332263	MS.gene76497:CDS	55.0%
!!	TGGTGGTGGATGGCAATGTG+TGG	+	chr2.3:2332240-2332259	MS.gene76497:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	2331903	2332360	2331903	ID=MS.gene76497
chr2.3	mRNA	2331903	2332360	2331903	ID=MS.gene76497.t1;Parent=MS.gene76497
chr2.3	exon	2331903	2332040	2331903	ID=MS.gene76497.t1.exon1;Parent=MS.gene76497.t1
chr2.3	CDS	2331903	2332040	2331903	ID=cds.MS.gene76497.t1;Parent=MS.gene76497.t1
chr2.3	exon	2332223	2332360	2332223	ID=MS.gene76497.t1.exon2;Parent=MS.gene76497.t1
chr2.3	CDS	2332223	2332360	2332223	ID=cds.MS.gene76497.t1;Parent=MS.gene76497.t1

Gene Sequence

>MS.gene76497

ATGTTGGAAGGTAAAGCTATGATAGAAGATACTGACATGCCAACTAAGATGCAGATTCAAGCCATGGCTTATGCTTCTGAAGCTCTTGATCTATATGATGTTATTGATTGCAAATCCATTGCTGCCCACATAAAAAAGGTTACATTTTTCTCTTCAATTATTGTATTATACACACAACACAACATCATTAAGTTGTTCATTTATAGATGTATATATAGTAATCACTTATTTATTAAGAAACTAAATAACTGACTAAACATGTATTATAAAGATAAATTGATATTCAAATATTATTTATTTTTCTTTGAATGTCATGTTAGGAGTTTGATGCAAGATATGGTGGTGGATGGCAATGTGTGGTAGGTTCAAGTTTTGGGTGTTTTTTCACTCATTCTAAGGGAACTTTCATATACTTCACTCTTGAGACTCTCAATTTCCTTATTTTCAAAGGAGTTTGA

Protein sequence

>MS.gene76497.t1

MLEGKAMIEDTDMPTKMQIQAMAYASEALDLYDVIDCKSIAAHIKKEFDARYGGGWQCVVGSSFGCFFTHSKGTFIYFTLETLNFLIFKGV