AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77048

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77048.t1 MTR_4g028960 96.586 703 22 1 1 703 1 701 0.0 1388
MS.gene77048.t1 MTR_2g018010 36.905 672 384 15 20 667 20 675 1.32e-120 377
MS.gene77048.t1 MTR_5g083030 30.823 717 407 17 1 667 8 685 1.03e-90 298
MS.gene77048.t1 MTR_4g107010 29.784 695 411 19 7 668 16 666 1.15e-81 275
MS.gene77048.t1 MTR_3g078160 29.275 690 424 16 5 668 8 659 2.92e-80 270
MS.gene77048.t1 MTR_3g078160 29.275 690 424 16 5 668 8 659 3.71e-80 269
MS.gene77048.t1 MTR_3g078160 31.900 558 323 13 5 543 8 527 1.79e-73 248
MS.gene77048.t1 MTR_1g094215 31.554 637 398 16 38 667 15 620 7.26e-72 246
MS.gene77048.t1 MTR_4g485520 33.003 606 326 23 95 667 90 648 3.51e-66 231
MS.gene77048.t1 MTR_4g485520 38.614 404 206 13 280 667 103 480 1.32e-64 222
MS.gene77048.t1 MTR_1g094215 38.520 392 219 10 281 667 63 437 6.53e-63 216
MS.gene77048.t1 MTR_4g063800 30.294 647 410 20 35 667 9 628 1.67e-62 221
MS.gene77048.t1 MTR_7g078330 31.399 586 361 16 95 667 85 642 2.73e-61 217
MS.gene77048.t1 MTR_7g059405 37.113 388 223 10 281 665 263 632 4.22e-59 211
MS.gene77048.t1 MTR_5g034440 27.273 693 434 18 7 668 14 667 1.37e-58 210
MS.gene77048.t1 MTR_7g053260 33.162 389 240 10 283 667 79 451 1.76e-49 180
MS.gene77048.t1 MTR_7g116600 32.648 389 243 9 283 667 79 452 9.38e-48 175
MS.gene77048.t1 MTR_1g079450 31.234 397 249 10 273 662 54 433 2.47e-41 157
MS.gene77048.t1 MTR_3g085610 26.773 437 257 10 283 666 281 707 5.21e-37 148
MS.gene77048.t1 MTR_8g080280 26.116 448 248 12 283 667 283 710 1.73e-35 144
MS.gene77048.t1 MTR_2g011140 29.504 383 244 8 287 666 20 379 4.82e-30 123
MS.gene77048.t1 MTR_4g085720 26.415 424 254 14 282 680 14 404 4.13e-26 112
MS.gene77048.t1 MTR_4g091880 28.462 390 247 7 282 666 5 367 4.73e-26 111
MS.gene77048.t1 MTR_8g092870 25.845 414 261 15 278 666 6 398 1.46e-25 110
MS.gene77048.t1 MTR_4g091880 29.156 391 215 15 282 666 5 339 6.66e-24 104
MS.gene77048.t1 MTR_7g097020 33.117 308 181 10 376 674 472 763 8.07e-24 107
MS.gene77048.t1 MTR_7g097020 59.420 69 28 0 286 354 245 313 2.76e-23 105
MS.gene77048.t1 MTR_3g466220 55.952 84 36 1 272 354 222 305 1.04e-23 107
MS.gene77048.t1 MTR_3g466220 35.424 271 161 8 408 674 574 834 1.25e-21 100
MS.gene77048.t1 MTR_3g466220 55.952 84 36 1 272 354 201 284 1.06e-23 107
MS.gene77048.t1 MTR_3g466220 35.424 271 161 8 408 674 553 813 1.57e-21 100
MS.gene77048.t1 MTR_3g065080 25.915 328 186 12 279 561 33 348 1.43e-23 104
MS.gene77048.t1 MTR_1g069845 62.687 67 25 0 283 349 125 191 2.46e-23 105
MS.gene77048.t1 MTR_1g069845 31.457 302 188 10 380 674 317 606 5.64e-21 98.2
MS.gene77048.t1 MTR_1g069845 62.687 67 25 0 283 349 246 312 5.67e-23 104
MS.gene77048.t1 MTR_1g069845 31.788 302 187 10 380 674 438 727 2.97e-21 99.4
MS.gene77048.t1 MTR_1g069845 62.687 67 25 0 283 349 242 308 6.08e-23 104
MS.gene77048.t1 MTR_1g069845 31.788 302 187 10 380 674 434 723 2.42e-21 99.8
MS.gene77048.t1 MTR_5g048050 51.948 77 37 0 277 353 20 96 1.85e-22 101
MS.gene77048.t1 MTR_5g020570 22.907 454 279 12 275 666 281 725 3.13e-22 102
MS.gene77048.t1 MTR_5g020570 22.907 454 279 12 275 666 266 710 4.45e-22 102
MS.gene77048.t1 MTR_6g071340 51.316 76 37 0 278 353 1 76 4.73e-22 99.8
MS.gene77048.t1 MTR_1g093965 29.834 181 95 5 295 470 1 154 8.41e-22 94.4
MS.gene77048.t1 MTR_5g077510 47.368 76 40 0 278 353 31 106 9.75e-22 99.4
MS.gene77048.t1 MTR_8g077205 21.105 507 307 11 229 666 114 596 1.07e-21 100
MS.gene77048.t1 MTR_8g077205 21.105 507 307 11 229 666 226 708 1.21e-21 100
MS.gene77048.t1 MTR_3g103050 30.662 287 189 6 385 667 50 330 1.30e-21 97.4
MS.gene77048.t1 MTR_2g087350 32.243 214 121 7 279 486 3 198 2.78e-21 97.4
MS.gene77048.t1 MTR_7g106340 28.947 304 170 12 279 559 5 285 1.58e-20 95.1
MS.gene77048.t1 MTR_3g095730 26.139 417 254 18 278 666 9 399 1.78e-20 95.1
MS.gene77048.t1 MTR_1g093995 29.282 181 96 5 295 470 1 154 3.05e-20 89.7
MS.gene77048.t1 MTR_1g018180 32.857 280 163 8 398 667 72 336 8.97e-20 91.7
MS.gene77048.t1 MTR_3g096370 51.429 70 34 0 279 348 7 76 1.69e-19 92.0
MS.gene77048.t1 MTR_1g056840 26.888 331 166 14 275 559 2 302 2.39e-18 88.6
MS.gene77048.t1 MTR_1g056880 24.714 437 258 16 275 664 2 414 5.39e-18 87.8
MS.gene77048.t1 MTR_5g015210 25.474 369 225 11 276 628 60 394 6.83e-18 87.4
MS.gene77048.t1 MTR_1g056870 37.195 164 79 7 275 432 2 147 4.85e-17 84.7
MS.gene77048.t1 MTR_1g017770 29.515 227 116 8 282 498 45 237 1.31e-16 83.2
MS.gene77048.t1 MTR_2g026130 31.900 279 145 10 408 666 196 449 1.88e-16 83.2
MS.gene77048.t1 MTR_1g056910 27.000 300 172 13 279 559 3 274 8.24e-16 80.5
MS.gene77048.t1 MTR_1g100820 50.000 66 33 0 283 348 649 714 9.62e-16 81.6
MS.gene77048.t1 MTR_1g100820 50.000 66 33 0 283 348 647 712 9.76e-16 81.6
MS.gene77048.t1 MTR_8g039560 30.128 312 182 12 375 665 1 297 4.21e-15 77.8
MS.gene77048.t1 MTR_7g077780 48.529 68 35 0 283 350 827 894 6.63e-15 79.3
MS.gene77048.t1 MTR_7g117890 27.519 258 151 8 109 348 82 321 1.61e-14 78.2
MS.gene77048.t1 MTR_8g027140 22.707 458 291 15 109 550 83 493 1.94e-14 77.8
MS.gene77048.t1 MTR_4g051515 34.177 158 85 7 279 433 3 144 2.31e-14 76.3
MS.gene77048.t1 MTR_4g125930 46.377 69 36 1 283 351 492 559 3.07e-13 73.2
MS.gene77048.t1 MTR_3g115670 24.679 389 219 14 264 584 13 395 3.31e-13 73.6
MS.gene77048.t1 MTR_4g125930 46.377 69 36 1 283 351 692 759 4.12e-13 73.2
MS.gene77048.t1 MTR_5g032010 44.776 67 37 0 282 348 732 798 1.07e-12 72.0
MS.gene77048.t1 MTR_2g096850 44.000 75 41 1 277 350 735 809 1.24e-12 71.6
MS.gene77048.t1 MTR_2g096850 44.000 75 41 1 277 350 584 658 1.24e-12 71.6
MS.gene77048.t1 MTR_1g090320 43.056 72 37 3 283 352 514 583 2.06e-11 68.2
MS.gene77048.t1 MTR_1g090320 43.056 72 37 3 283 352 516 585 2.11e-11 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77048.t1 AT5G01830 52.830 689 278 11 20 701 21 669 0.0 684
MS.gene77048.t1 AT1G29340 35.474 623 360 11 106 696 115 727 2.85e-110 350
MS.gene77048.t1 AT1G60190 30.159 693 412 22 7 668 14 665 1.16e-76 260
MS.gene77048.t1 AT3G54850 31.429 630 381 19 52 667 30 622 1.51e-71 245
MS.gene77048.t1 AT5G42340 31.294 572 347 16 109 667 118 656 2.99e-66 231
MS.gene77048.t1 AT3G46510 28.550 655 417 15 33 669 10 631 3.70e-66 231
MS.gene77048.t1 AT1G23030 31.029 622 354 16 66 667 43 609 1.10e-65 228
MS.gene77048.t1 AT1G10560 28.307 703 423 21 7 668 14 676 2.18e-65 229
MS.gene77048.t1 AT1G71020 30.215 652 383 17 42 667 15 620 2.30e-59 211
MS.gene77048.t1 AT2G28830 29.806 567 365 12 119 668 13 563 2.93e-59 210
MS.gene77048.t1 AT1G71020 33.470 487 270 13 207 667 14 472 6.86e-59 206
MS.gene77048.t1 AT3G07360 32.927 410 246 13 271 667 59 452 4.49e-48 176
MS.gene77048.t1 AT1G67530 26.912 680 375 23 90 666 46 706 2.23e-41 162
MS.gene77048.t1 AT1G67530 26.912 680 375 23 90 666 46 706 2.23e-41 162
MS.gene77048.t1 AT1G67530 26.912 680 375 23 90 666 46 706 2.23e-41 162
MS.gene77048.t1 AT1G27910 24.405 672 399 20 90 666 46 703 3.29e-36 146
MS.gene77048.t1 AT1G24330 23.923 673 403 18 90 666 46 705 6.65e-34 139
MS.gene77048.t1 AT1G24330 23.923 673 403 18 90 666 46 705 6.65e-34 139
MS.gene77048.t1 AT4G21350 31.795 390 233 10 282 665 5 367 7.31e-33 131
MS.gene77048.t1 AT1G67530 27.579 475 234 18 90 469 46 505 9.38e-30 124
MS.gene77048.t1 AT1G49780 29.238 407 253 12 278 666 10 399 9.90e-28 117
MS.gene77048.t1 AT3G19380 29.558 362 221 12 278 622 10 354 1.02e-27 117
MS.gene77048.t1 AT5G18320 28.856 402 253 11 282 671 72 452 1.44e-27 117
MS.gene77048.t1 AT5G67340 33.208 265 158 8 409 666 445 697 1.28e-24 110
MS.gene77048.t1 AT5G67340 53.425 73 34 0 282 354 240 312 1.95e-20 96.7
MS.gene77048.t1 AT5G67340 33.208 265 158 8 409 666 445 697 1.28e-24 110
MS.gene77048.t1 AT5G67340 53.425 73 34 0 282 354 240 312 1.95e-20 96.7
MS.gene77048.t1 AT1G66160 25.858 437 261 16 275 686 26 424 2.05e-24 107
MS.gene77048.t1 AT5G37490 24.939 413 255 14 279 665 28 411 1.15e-23 105
MS.gene77048.t1 AT2G23140 32.509 283 175 8 397 674 556 827 1.02e-22 104
MS.gene77048.t1 AT2G23140 30.859 256 145 7 119 366 86 317 2.30e-22 103
MS.gene77048.t1 AT2G23140 32.509 283 175 8 397 674 553 824 1.12e-22 103
MS.gene77048.t1 AT2G23140 30.859 256 145 7 119 366 83 314 2.59e-22 102
MS.gene77048.t1 AT2G23140 32.509 283 175 8 397 674 553 824 1.12e-22 103
MS.gene77048.t1 AT2G23140 30.859 256 145 7 119 366 83 314 2.59e-22 102
MS.gene77048.t1 AT2G23140 32.862 283 174 9 397 674 364 635 2.19e-22 102
MS.gene77048.t1 AT2G23140 42.105 95 55 0 272 366 31 125 1.14e-21 100
MS.gene77048.t1 AT2G23140 32.862 283 174 9 397 674 364 635 2.19e-22 102
MS.gene77048.t1 AT2G23140 42.105 95 55 0 272 366 31 125 1.14e-21 100
MS.gene77048.t1 AT3G54790 54.795 73 33 0 282 354 202 274 7.20e-22 101
MS.gene77048.t1 AT3G54790 30.515 272 158 7 408 666 459 712 1.89e-18 90.5
MS.gene77048.t1 AT3G54790 54.795 73 33 0 282 354 238 310 9.43e-22 100
MS.gene77048.t1 AT3G54790 30.515 272 158 7 408 666 495 748 1.07e-18 91.3
MS.gene77048.t1 AT3G54790 54.795 73 33 0 282 354 238 310 9.43e-22 100
MS.gene77048.t1 AT3G54790 30.515 272 158 7 408 666 495 748 1.07e-18 91.3
MS.gene77048.t1 AT5G64660 50.667 75 37 0 274 348 2 76 1.20e-20 95.5
MS.gene77048.t1 AT3G49810 25.967 362 219 10 282 628 64 391 2.31e-20 95.1
MS.gene77048.t1 AT3G01400 29.508 305 182 8 385 675 63 348 1.58e-19 91.3
MS.gene77048.t1 AT3G52450 52.778 72 33 1 279 349 4 75 2.20e-19 92.0
MS.gene77048.t1 AT5G65920 24.586 362 224 10 282 628 60 387 2.40e-19 92.0
MS.gene77048.t1 AT5G65920 24.586 362 224 10 282 628 60 387 2.40e-19 92.0
MS.gene77048.t1 AT2G35930 31.551 187 97 5 279 458 9 171 3.26e-19 91.3
MS.gene77048.t1 AT5G18340 27.119 295 192 9 271 557 63 342 5.31e-19 90.9
MS.gene77048.t1 AT3G07360 28.829 333 212 12 344 667 1 317 8.40e-19 88.6
MS.gene77048.t1 AT3G07360 28.829 333 212 12 344 667 1 317 8.40e-19 88.6
MS.gene77048.t1 AT3G07360 28.829 333 212 12 344 667 1 317 8.40e-19 88.6
MS.gene77048.t1 AT3G07360 28.829 333 212 12 344 667 1 317 8.40e-19 88.6
MS.gene77048.t1 AT3G07360 28.829 333 212 12 344 667 1 317 8.40e-19 88.6
MS.gene77048.t1 AT3G07360 28.829 333 212 12 344 667 1 317 8.40e-19 88.6
MS.gene77048.t1 AT3G07360 28.829 333 212 12 344 667 1 317 8.40e-19 88.6
MS.gene77048.t1 AT3G07360 28.829 333 212 12 344 667 1 317 8.40e-19 88.6
MS.gene77048.t1 AT3G07360 28.829 333 212 12 344 667 1 317 8.40e-19 88.6
MS.gene77048.t1 AT3G18710 50.725 69 34 0 280 348 10 78 8.59e-19 89.7
MS.gene77048.t1 AT5G09800 52.239 67 32 0 282 348 11 77 1.11e-18 89.4
MS.gene77048.t1 AT5G58680 29.137 278 183 7 395 667 71 339 5.74e-17 83.6
MS.gene77048.t1 AT1G01660 51.515 66 32 0 283 348 499 564 2.87e-16 83.2
MS.gene77048.t1 AT1G01660 51.515 66 32 0 283 348 498 563 2.91e-16 83.2
MS.gene77048.t1 AT1G66160 24.027 437 253 17 275 686 26 408 1.39e-15 80.1
MS.gene77048.t1 AT2G45910 40.449 89 53 0 264 352 745 833 5.65e-15 79.3
MS.gene77048.t1 AT2G45910 40.449 89 53 0 264 352 745 833 5.65e-15 79.3
MS.gene77048.t1 AT1G01680 44.737 76 42 0 273 348 224 299 8.89e-15 76.3
MS.gene77048.t1 AT2G45920 48.485 66 34 0 283 348 326 391 1.90e-14 76.3
MS.gene77048.t1 AT3G11840 44.737 76 40 2 279 353 7 81 1.95e-13 73.6
MS.gene77048.t1 AT1G08315 26.888 331 188 14 377 677 4 310 7.11e-13 70.9
MS.gene77048.t1 AT1G56040 46.032 63 34 0 283 345 354 416 8.73e-13 71.2
MS.gene77048.t1 AT4G16490 31.183 279 149 10 408 666 206 461 9.53e-13 71.6
MS.gene77048.t1 AT3G49060 41.892 74 43 0 275 348 695 768 1.13e-12 72.0
MS.gene77048.t1 AT3G49060 41.892 74 43 0 275 348 728 801 1.15e-12 72.0
MS.gene77048.t1 AT3G49060 41.892 74 43 0 275 348 718 791 1.21e-12 72.0
MS.gene77048.t1 AT5G57035 43.939 66 37 0 283 348 709 774 2.11e-12 71.2
MS.gene77048.t1 AT3G61390 43.939 66 37 0 283 348 279 344 3.73e-12 68.9
MS.gene77048.t1 AT3G61390 43.939 66 37 0 283 348 354 419 6.01e-12 68.9
MS.gene77048.t1 AT2G19410 44.928 69 37 1 280 348 709 776 2.05e-11 67.8
MS.gene77048.t1 AT2G19410 44.928 69 37 1 280 348 744 811 2.11e-11 67.8
MS.gene77048.t1 AT2G19410 44.928 69 37 1 280 348 723 790 2.22e-11 67.8
MS.gene77048.t1 AT5G40140 25.795 283 177 10 399 666 242 506 3.59e-11 66.6
MS.gene77048.t1 AT5G62560 27.612 268 172 6 409 666 258 513 7.61e-11 65.9

Find 174 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGTTAAAGTTAGTGATATT+TGG 0.169459 4.3:-70750979 None:intergenic
ACACCATGGAGCTGCGTTTA+AGG 0.211733 4.3:-70751040 None:intergenic
CGTTTATGAGCTTCGGGTTT+TGG 0.217734 4.3:+70752124 MS.gene77048:CDS
ATTAAGCTTTGGATGGATTC+TGG 0.220926 4.3:+70751843 MS.gene77048:CDS
GGTTAAAGTTAGTGATATTT+GGG 0.221812 4.3:-70750978 None:intergenic
TTCAAGTTCATTTCTTGAAT+TGG 0.244410 4.3:-70751354 None:intergenic
CTGCGTTTAAGGAACATATT+TGG 0.265739 4.3:-70751029 None:intergenic
CATACCACCGTTAACTTTAA+CGG 0.270519 4.3:-70751994 None:intergenic
AAGGGTGATTTGGGAGTTAA+TGG 0.283097 4.3:+70752829 MS.gene77048:CDS
TGAGAAATATGATAGCTATT+TGG 0.284416 4.3:+70751931 MS.gene77048:CDS
GCTACTGTGTTTAGTTTAAC+TGG 0.293033 4.3:+70752395 MS.gene77048:CDS
CTGATATTGAATTACGAATT+AGG 0.306908 4.3:-70751134 None:intergenic
ACGTCGGTGGGCGGCGGGTT+TGG 0.311205 4.3:+70752904 MS.gene77048:CDS
TGGTGTGGAAAGGGTGATTT+GGG 0.315581 4.3:+70752820 MS.gene77048:CDS
TGGTGGTTCCGGCGGATTAC+CGG 0.317373 4.3:+70751748 MS.gene77048:CDS
ATGGTGTGGAAAGGGTGATT+TGG 0.329462 4.3:+70752819 MS.gene77048:CDS
CGGGAAATGATGGCCGTTTA+CGG 0.340552 4.3:+70751706 MS.gene77048:CDS
GGCGGTTGCGGCAGCGTTTG+TGG 0.341028 4.3:+70752670 MS.gene77048:CDS
GGAGATGACAGCGGGGATTA+TGG 0.342183 4.3:+70752586 MS.gene77048:CDS
GCGGTTGCGGCAGCGTTTGT+GGG 0.350026 4.3:+70752671 MS.gene77048:CDS
GATAATTTCAACAAATTAAC+CGG 0.356215 4.3:+70751303 MS.gene77048:CDS
CTGCTCGAAGATTGTTCTAA+TGG 0.358850 4.3:+70751240 MS.gene77048:CDS
AAAATTGGGGACGGTGTTAA+GGG 0.365091 4.3:+70752700 MS.gene77048:CDS
AAATGTATATTGTGCTTCAT+AGG 0.366592 4.3:+70751208 MS.gene77048:CDS
TGGCGTCGTTTATGAGCTTC+GGG 0.372444 4.3:+70752118 MS.gene77048:CDS
GTCGTTGGAATTGGTGGTTC+CGG 0.372795 4.3:+70751737 MS.gene77048:CDS
ATGGCGTCGTTTATGAGCTT+CGG 0.373212 4.3:+70752117 MS.gene77048:CDS
CGCATCAGTTCAAGACTTAT+CGG 0.383935 4.3:-70751774 None:intergenic
GAACAGAAAATTCCATTTGA+AGG 0.385995 4.3:+70751960 MS.gene77048:CDS
TCAGAAACGAAGAATTTGTT+AGG 0.387591 4.3:+70751420 MS.gene77048:CDS
AGCGAATAAGACAAGGATAA+TGG 0.391442 4.3:+70752295 MS.gene77048:CDS
TTAAGCTTTGGATGGATTCT+GGG 0.392841 4.3:+70751844 MS.gene77048:CDS
GAACAGAAACAACGTCGTTT+CGG 0.392901 4.3:-70751461 None:intergenic
GCGGAATCAGTCGTTGGAAT+TGG 0.393295 4.3:+70751728 MS.gene77048:CDS
ATGGCGGTGGGGAGTGTTAG+AGG 0.398283 4.3:+70752848 MS.gene77048:CDS
GCGGAGCTTGCGGCAATTCA+TGG 0.399152 4.3:+70752800 MS.gene77048:CDS
GATCCTGTCGTCGTGGCAAC+AGG 0.400951 4.3:+70751798 MS.gene77048:CDS
GATTTGGGAGTTAATGGCGG+TGG 0.402348 4.3:+70752835 MS.gene77048:CDS
GTATTAACTCCGTGTGGGCC+AGG 0.406263 4.3:-70751896 None:intergenic
ATCGTGTTTCGATTAAGCTT+TGG 0.414902 4.3:+70751832 MS.gene77048:CDS
TGGGCGGCGGGTTTGGATGG+TGG 0.427448 4.3:+70752911 MS.gene77048:CDS
GGCGGTAATGCCGGAGGAGG+CGG 0.437841 4.3:+70752607 MS.gene77048:CDS
GATGATGGTGGTTTAGTAGT+TGG 0.439712 4.3:-70750934 None:intergenic
ACGACTGATTCCGCCGTAAA+CGG 0.442880 4.3:-70751719 None:intergenic
GACGTTGTTTCTGTTCTAGA+AGG 0.453096 4.3:+70751468 MS.gene77048:CDS
GGGAGGGATCAGAGAGGGCG+AGG 0.456168 4.3:+70752720 MS.gene77048:CDS
CACAACACGTGTCCTAAAAC+AGG 0.458381 4.3:+70751867 MS.gene77048:CDS
GGGGATTATGGCGGTAATGC+CGG 0.458721 4.3:+70752598 MS.gene77048:CDS
CGGTGGGGAGTGTTAGAGGG+AGG 0.459782 4.3:+70752852 MS.gene77048:CDS
GAACTTGAATGATGATGTAT+TGG 0.460758 4.3:+70751368 MS.gene77048:CDS
TATGACAAATAAGACAGCTT+TGG 0.462456 4.3:+70752013 MS.gene77048:CDS
GGGGCCGTGGAGATGACAGC+GGG 0.467255 4.3:+70752578 MS.gene77048:CDS
ATTTGGGAGTTAATGGCGGT+GGG 0.475030 4.3:+70752836 MS.gene77048:CDS
ATATTTCTCAAAGCATGGTT+AGG 0.475353 4.3:-70751918 None:intergenic
GGTTAAGCGTGGAGGATTAG+TGG 0.478476 4.3:+70752649 MS.gene77048:CDS
AGTATTGTCACCGCCTCCTC+CGG 0.479400 4.3:-70752617 None:intergenic
TTCAAGAAGAAATTCAGAAT+CGG 0.481818 4.3:+70751592 MS.gene77048:CDS
CGAGCTTGTATAGCTGAAGC+TGG 0.483143 4.3:+70752167 MS.gene77048:CDS
AACGACGCCGTTTCTGATGA+TGG 0.489185 4.3:-70750949 None:intergenic
AGCGAAGAGGGACGCATTAG+CGG 0.492282 4.3:+70752502 MS.gene77048:CDS
GCGTCCCTCTTCGCTCCCTC+AGG 0.492921 4.3:-70752494 None:intergenic
TGTCCTGTTGCCACGACGAC+AGG 0.493205 4.3:-70751801 None:intergenic
TCAGAGATTGTTGCGTTGAT+CGG 0.493804 4.3:+70751633 MS.gene77048:CDS
AGAAGGAATCAAGAGAGAAA+TGG 0.494151 4.3:+70751485 MS.gene77048:CDS
GAGTGTTAGAGGGAGGAGGA+AGG 0.494415 4.3:+70752859 MS.gene77048:CDS
TTTGCATCTCGCAGCGGATA+GGG 0.494746 4.3:+70752529 MS.gene77048:CDS
TGTGCTAATGAAGTTGTAGT+TGG 0.496874 4.3:-70752995 None:intergenic
GTTGGTAGAGAGTGGGGCCG+TGG 0.498825 4.3:+70752565 MS.gene77048:CDS
GGGTTTGGATGGTGGTGAAA+CGG 0.499778 4.3:+70752919 MS.gene77048:CDS
TATTAACTCCGTGTGGGCCA+GGG 0.501113 4.3:-70751895 None:intergenic
GCTACCGTTTCACCTTCAAA+TGG 0.501489 4.3:-70751972 None:intergenic
GGTGCAACGACGGCGTCGAC+GGG 0.502990 4.3:+70751687 MS.gene77048:CDS
GACACGAATCGTTAGTGGAT+TGG 0.503002 4.3:+70752451 MS.gene77048:CDS
CGGTGGGCGGCGGGTTTGGA+TGG 0.505339 4.3:+70752908 MS.gene77048:CDS
TGTGTTTAGTTTAACTGGAG+TGG 0.506633 4.3:+70752400 MS.gene77048:CDS
TGGAGGATTAGTGGCGGTTG+CGG 0.506660 4.3:+70752658 MS.gene77048:CDS
AATTCCATTTGAAGGTGAAA+CGG 0.509042 4.3:+70751968 MS.gene77048:CDS
ACGGTAGCCGTTAAAGTTAA+CGG 0.510244 4.3:+70751987 MS.gene77048:CDS
TGGTGCAACGACGGCGTCGA+CGG 0.513053 4.3:+70751686 MS.gene77048:CDS
CGTTAGTGGATTGGTTGAGT+TGG 0.514133 4.3:+70752460 MS.gene77048:CDS
AGGAAGACACGAATCGTTAG+TGG 0.516476 4.3:+70752446 MS.gene77048:CDS
GAATCAGTCGTTGGAATTGG+TGG 0.520402 4.3:+70751731 MS.gene77048:CDS
TAGGGAGACGGTGGGAAGGT+TGG 0.520508 4.3:+70752547 MS.gene77048:CDS
TGGGGAGTGTTAGAGGGAGG+AGG 0.524460 4.3:+70752855 MS.gene77048:CDS
GGAGGGATCAGAGAGGGCGA+GGG 0.524599 4.3:+70752721 MS.gene77048:CDS
TAACGGTTCTTTAGCATCGA+TGG 0.527793 4.3:+70752085 MS.gene77048:CDS
TTCGCAAAATCCGAGCCTAC+AGG 0.528569 4.3:+70752232 MS.gene77048:CDS
ACGGCGTCGACGGGAAATGA+TGG 0.528688 4.3:+70751696 MS.gene77048:CDS
TATGGCGGTAATGCCGGAGG+AGG 0.529298 4.3:+70752604 MS.gene77048:CDS
TTGTGAGGTGTAATAGAGAA+TGG 0.534465 4.3:-70751005 None:intergenic
ATTGGGGACGGTGTTAAGGG+AGG 0.534820 4.3:+70752703 MS.gene77048:CDS
TGGACCTGAGGGAGCGAAGA+GGG 0.535333 4.3:+70752490 MS.gene77048:CDS
ATACGTAAAACGCACCTCCT+CGG 0.542514 4.3:+70751096 MS.gene77048:CDS
GATAAGTCTTGAACTGATGC+GGG 0.544369 4.3:+70751776 MS.gene77048:CDS
TGTTTCGATTAAGCTTTGGA+TGG 0.545411 4.3:+70751836 MS.gene77048:CDS
AGAGATTGTGGCGGAGCTTG+CGG 0.545486 4.3:+70752790 MS.gene77048:CDS
TTGGATGGTGGTGAAACGGA+GGG 0.546989 4.3:+70752923 MS.gene77048:CDS
TGGGAAGGTTGGTAGAGAGT+GGG 0.549322 4.3:+70752558 MS.gene77048:CDS
GGTGTAATAGAGAATGGAGG+AGG 0.550947 4.3:-70750999 None:intergenic
GGAAGGCGGCGACGTTGTTG+AGG 0.554359 4.3:+70752876 MS.gene77048:CDS
CGATAAGTCTTGAACTGATG+CGG 0.556842 4.3:+70751775 MS.gene77048:CDS
CGGCAATTCATGGTGTGGAA+AGG 0.558496 4.3:+70752810 MS.gene77048:CDS
TAAACCACCATCATCAGAAA+CGG 0.559799 4.3:+70750942 MS.gene77048:CDS
GTGGGAAGGTTGGTAGAGAG+TGG 0.562347 4.3:+70752557 MS.gene77048:CDS
AGGAACATATTTGGTTTGTG+AGG 0.564288 4.3:-70751020 None:intergenic
CTATCATATTTCTCAAAGCA+TGG 0.564762 4.3:-70751923 None:intergenic
ATCGGACACCGGTAATCCGC+CGG 0.567196 4.3:-70751756 None:intergenic
GCATCGATGGAAGAGACAAA+TGG 0.568990 4.3:+70752098 MS.gene77048:CDS
GGCAATTCATGGTGTGGAAA+GGG 0.572572 4.3:+70752811 MS.gene77048:CDS
AAGAAGAGTTGATAATTCAC+CGG 0.577670 4.3:-70751322 None:intergenic
TTAGTAGTTGGAAATGTTTG+AGG 0.577855 4.3:-70750922 None:intergenic
GTTCCTTAAACGCAGCTCCA+TGG 0.581251 4.3:+70751037 MS.gene77048:CDS
GGGAAGGTTGGTAGAGAGTG+GGG 0.582589 4.3:+70752559 MS.gene77048:CDS
AGAGAGGGCGAGGGAGAGTG+CGG 0.583035 4.3:+70752730 MS.gene77048:CDS
GGTGATTTGGGAGTTAATGG+CGG 0.585091 4.3:+70752832 MS.gene77048:CDS
CGGATAGGGAGACGGTGGGA+AGG 0.585973 4.3:+70752543 MS.gene77048:CDS
CGCAGCGGATAGGGAGACGG+TGG 0.589169 4.3:+70752538 MS.gene77048:CDS
GTTGGAATTGGTGGTTCCGG+CGG 0.589268 4.3:+70751740 MS.gene77048:CDS
TGTTAGAGGGAGGAGGAAGG+CGG 0.591852 4.3:+70752862 MS.gene77048:CDS
TCCTAAAACAGGTCAAACCC+TGG 0.592266 4.3:+70751878 MS.gene77048:CDS
AGGTCAAACCCTGGCCCACA+CGG 0.592317 4.3:+70751887 MS.gene77048:CDS
CGGTTGCGGCAGCGTTTGTG+GGG 0.593066 4.3:+70752672 MS.gene77048:CDS
TGAGGTGTAATAGAGAATGG+AGG 0.594765 4.3:-70751002 None:intergenic
TTTGGATGGTGGTGAAACGG+AGG 0.595577 4.3:+70752922 MS.gene77048:CDS
TTTACGGCGGAATCAGTCGT+TGG 0.597846 4.3:+70751722 MS.gene77048:CDS
GGTTAGGTATTAACTCCGTG+TGG 0.601915 4.3:-70751902 None:intergenic
TGGCGGTGGGGAGTGTTAGA+GGG 0.603345 4.3:+70752849 MS.gene77048:CDS
AACAAGTTGACGTTGACTAA+CGG 0.605175 4.3:+70752068 MS.gene77048:CDS
GAAAGGTGGATCAGAGATTG+TGG 0.605941 4.3:+70752778 MS.gene77048:CDS
AATCCCCGCTGTCATCTCCA+CGG 0.607104 4.3:-70752582 None:intergenic
GTTAAAGTTAGTGATATTTG+GGG 0.607708 4.3:-70750977 None:intergenic
TGTTAGTACATCAACTACCA+TGG 0.608462 4.3:+70752967 MS.gene77048:CDS
ACTGCATTAACCTGTAGGCT+CGG 0.609903 4.3:-70752242 None:intergenic
GTTAAGGGAGGGATCAGAGA+GGG 0.610803 4.3:+70752715 MS.gene77048:CDS
TTTAAGATCGGGTGCCACGT+GGG 0.611826 4.3:+70752352 MS.gene77048:CDS
TTGTAACTGCATTAACCTGT+AGG 0.615427 4.3:-70752247 None:intergenic
TTGGGGACGGTGTTAAGGGA+GGG 0.620299 4.3:+70752704 MS.gene77048:CDS
GTGACAATGTGTAGGAAAGG+TGG 0.623592 4.3:+70752764 MS.gene77048:CDS
TTTGGAAGCAACAAGAATGA+CGG 0.625454 4.3:+70752031 MS.gene77048:CDS
TCTCGCAGCGGATAGGGAGA+CGG 0.625914 4.3:+70752535 MS.gene77048:CDS
GATTATGGCGGTAATGCCGG+AGG 0.627766 4.3:+70752601 MS.gene77048:CDS
GCTTGCGGCAATTCATGGTG+TGG 0.630345 4.3:+70752805 MS.gene77048:CDS
AAGATCGGGTGCCACGTGGG+AGG 0.630647 4.3:+70752355 MS.gene77048:CDS
CGACGCTTGTGACAATGTGT+AGG 0.639061 4.3:+70752756 MS.gene77048:CDS
TTTGGGAGTTAATGGCGGTG+GGG 0.641101 4.3:+70752837 MS.gene77048:CDS
CTTGAAGCAGTGGTTAAGCG+TGG 0.642024 4.3:+70752638 MS.gene77048:CDS
GGTGACAATACTTGAAGCAG+TGG 0.642876 4.3:+70752628 MS.gene77048:CDS
GTTAGGTATTAACTCCGTGT+GGG 0.650428 4.3:-70751901 None:intergenic
ACAAGGATAATGGAGACTGA+AGG 0.650963 4.3:+70752305 MS.gene77048:CDS
TGGGGCCGTGGAGATGACAG+CGG 0.650969 4.3:+70752577 MS.gene77048:CDS
GCAGCGGATAGGGAGACGGT+GGG 0.651330 4.3:+70752539 MS.gene77048:CDS
TTTAAAAGGAAACGACACCA+TGG 0.659696 4.3:-70751054 None:intergenic
TACTTGAAGCGAATAAGACA+AGG 0.661909 4.3:+70752288 MS.gene77048:CDS
CTTGTGACAATGTGTAGGAA+AGG 0.662139 4.3:+70752761 MS.gene77048:CDS
GTGGGAGGCTAAAGCAAATG+CGG 0.672161 4.3:+70752370 MS.gene77048:CDS
ATGGACCTGAGGGAGCGAAG+AGG 0.672284 4.3:+70752489 MS.gene77048:CDS
GACGCCGTTTCTGATGATGG+TGG 0.677070 4.3:-70750946 None:intergenic
GATGCGGGATCCTGTCGTCG+TGG 0.685189 4.3:+70751791 MS.gene77048:CDS
TAAGCGTGGAGGATTAGTGG+CGG 0.685593 4.3:+70752652 MS.gene77048:CDS
GGAGGCTAAAGCAAATGCGG+CGG 0.686035 4.3:+70752373 MS.gene77048:CDS
GATGACAGCGGGGATTATGG+CGG 0.687573 4.3:+70752589 MS.gene77048:CDS
GAAATGATGGCCGTTTACGG+CGG 0.687834 4.3:+70751709 MS.gene77048:CDS
GTTCAAGACTTATCGGACAC+CGG 0.688916 4.3:-70751767 None:intergenic
ATTTGCTTTAGCCTCCCACG+TGG 0.692219 4.3:-70752366 None:intergenic
AGTTGTAGTTGGAACAACCA+TGG 0.693372 4.3:-70752984 None:intergenic
GAAGCAGTGGTTAAGCGTGG+AGG 0.694054 4.3:+70752641 MS.gene77048:CDS
ACACATTGTCACAAGCGTCG+CGG 0.697841 4.3:-70752753 None:intergenic
CATCGAAAACGACACCGAGG+AGG 0.702271 4.3:-70751110 None:intergenic
AGGTGGATCAGAGATTGTGG+CGG 0.710219 4.3:+70752781 MS.gene77048:CDS
TGTTAAGGGAGGGATCAGAG+AGG 0.725744 4.3:+70752714 MS.gene77048:CDS
GGAAGCAACAAGAATGACGG+CGG 0.729408 4.3:+70752034 MS.gene77048:CDS
GGGCCGTGGAGATGACAGCG+GGG 0.731568 4.3:+70752579 MS.gene77048:CDS
GTAGCCGTTAAAGTTAACGG+TGG 0.777768 4.3:+70751990 MS.gene77048:CDS
GGTCATCGAAAACGACACCG+AGG 0.788291 4.3:-70751113 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATAATTTCAACAAATTAAC+CGG + chr4.3:70751303-70751322 MS.gene77048:CDS 20.0%
!!! AAGAGGAAATTTTGTTTAAA+AGG - chr4.3:70751071-70751090 None:intergenic 20.0%
!!! ATCTTCAATTTTTGAGAAAT+TGG + chr4.3:70751527-70751546 MS.gene77048:CDS 20.0%
!!! GAAGATAATTTTTCATCTTT+TGG - chr4.3:70751513-70751532 None:intergenic 20.0%
! AAATGTATATTGTGCTTCAT+AGG + chr4.3:70751208-70751227 MS.gene77048:CDS 25.0%
! AGGTTAAAGTTAGTGATATT+TGG - chr4.3:70750982-70751001 None:intergenic 25.0%
! CTGATATTGAATTACGAATT+AGG - chr4.3:70751137-70751156 None:intergenic 25.0%
! GGTTAAAGTTAGTGATATTT+GGG - chr4.3:70750981-70751000 None:intergenic 25.0%
! GTTAAAGTTAGTGATATTTG+GGG - chr4.3:70750980-70750999 None:intergenic 25.0%
! TGAGAAATATGATAGCTATT+TGG + chr4.3:70751931-70751950 MS.gene77048:CDS 25.0%
! TTCAAGAAGAAATTCAGAAT+CGG + chr4.3:70751592-70751611 MS.gene77048:CDS 25.0%
! TTTGTGGGGATAAAAAAATT+GGG + chr4.3:70752686-70752705 MS.gene77048:CDS 25.0%
!! TTCAAGTTCATTTCTTGAAT+TGG - chr4.3:70751357-70751376 None:intergenic 25.0%
AATTCCATTTGAAGGTGAAA+CGG + chr4.3:70751968-70751987 MS.gene77048:CDS 30.0%
ATATTTCTCAAAGCATGGTT+AGG - chr4.3:70751921-70751940 None:intergenic 30.0%
CTATCATATTTCTCAAAGCA+TGG - chr4.3:70751926-70751945 None:intergenic 30.0%
GAACAGAAAATTCCATTTGA+AGG + chr4.3:70751960-70751979 MS.gene77048:CDS 30.0%
GTTTGTGGGGATAAAAAAAT+TGG + chr4.3:70752685-70752704 MS.gene77048:CDS 30.0%
TATGACAAATAAGACAGCTT+TGG + chr4.3:70752013-70752032 MS.gene77048:CDS 30.0%
TTAGTAGTTGGAAATGTTTG+AGG - chr4.3:70750925-70750944 None:intergenic 30.0%
TTGTGGGGATAAAAAAATTG+GGG + chr4.3:70752687-70752706 MS.gene77048:CDS 30.0%
! GAACTTGAATGATGATGTAT+TGG + chr4.3:70751368-70751387 MS.gene77048:CDS 30.0%
! TCAGAAACGAAGAATTTGTT+AGG + chr4.3:70751420-70751439 MS.gene77048:CDS 30.0%
!! AAGAAGAGTTGATAATTCAC+CGG - chr4.3:70751325-70751344 None:intergenic 30.0%
!!! CGGTTTTTAAGTTATGAAGT+TGG + chr4.3:70752209-70752228 MS.gene77048:CDS 30.0%
!!! GAGCTATAGAGATTTTGATT+CGG + chr4.3:70752189-70752208 MS.gene77048:CDS 30.0%
AGAAGGAATCAAGAGAGAAA+TGG + chr4.3:70751485-70751504 MS.gene77048:CDS 35.0%
AGCGAATAAGACAAGGATAA+TGG + chr4.3:70752295-70752314 MS.gene77048:CDS 35.0%
ATCGTGTTTCGATTAAGCTT+TGG + chr4.3:70751832-70751851 MS.gene77048:CDS 35.0%
CATACCACCGTTAACTTTAA+CGG - chr4.3:70751997-70752016 None:intergenic 35.0%
CTGCGTTTAAGGAACATATT+TGG - chr4.3:70751032-70751051 None:intergenic 35.0%
TAAACCACCATCATCAGAAA+CGG + chr4.3:70750942-70750961 MS.gene77048:CDS 35.0%
TACTTGAAGCGAATAAGACA+AGG + chr4.3:70752288-70752307 MS.gene77048:CDS 35.0%
TGTGCTAATGAAGTTGTAGT+TGG - chr4.3:70752998-70753017 None:intergenic 35.0%
TGTTAGTACATCAACTACCA+TGG + chr4.3:70752967-70752986 MS.gene77048:CDS 35.0%
TTGTAACTGCATTAACCTGT+AGG - chr4.3:70752250-70752269 None:intergenic 35.0%
TTGTGAGGTGTAATAGAGAA+TGG - chr4.3:70751008-70751027 None:intergenic 35.0%
TTTAAAAGGAAACGACACCA+TGG - chr4.3:70751057-70751076 None:intergenic 35.0%
TTTGGAAGCAACAAGAATGA+CGG + chr4.3:70752031-70752050 MS.gene77048:CDS 35.0%
! AACAAGTTGACGTTGACTAA+CGG + chr4.3:70752068-70752087 MS.gene77048:CDS 35.0%
! GCTACTGTGTTTAGTTTAAC+TGG + chr4.3:70752395-70752414 MS.gene77048:CDS 35.0%
! TGTGTTTAGTTTAACTGGAG+TGG + chr4.3:70752400-70752419 MS.gene77048:CDS 35.0%
!! AGGAACATATTTGGTTTGTG+AGG - chr4.3:70751023-70751042 None:intergenic 35.0%
!! ATTAAGCTTTGGATGGATTC+TGG + chr4.3:70751843-70751862 MS.gene77048:CDS 35.0%
!! TGTTTCGATTAAGCTTTGGA+TGG + chr4.3:70751836-70751855 MS.gene77048:CDS 35.0%
!! TTAAGCTTTGGATGGATTCT+GGG + chr4.3:70751844-70751863 MS.gene77048:CDS 35.0%
ACGGTAGCCGTTAAAGTTAA+CGG + chr4.3:70751987-70752006 MS.gene77048:CDS 40.0%
AGTTGTAGTTGGAACAACCA+TGG - chr4.3:70752987-70753006 None:intergenic 40.0%
CGATAAGTCTTGAACTGATG+CGG + chr4.3:70751775-70751794 MS.gene77048:CDS 40.0%
CGCATCAGTTCAAGACTTAT+CGG - chr4.3:70751777-70751796 None:intergenic 40.0%
CTGCTCGAAGATTGTTCTAA+TGG + chr4.3:70751240-70751259 MS.gene77048:CDS 40.0%
CTTGTGACAATGTGTAGGAA+AGG + chr4.3:70752761-70752780 MS.gene77048:CDS 40.0%
GAACAGAAACAACGTCGTTT+CGG - chr4.3:70751464-70751483 None:intergenic 40.0%
GATAAGTCTTGAACTGATGC+GGG + chr4.3:70751776-70751795 MS.gene77048:CDS 40.0%
GGGGATAAAAAAATTGGGGA+CGG + chr4.3:70752691-70752710 MS.gene77048:CDS 40.0%
GTTAGGTATTAACTCCGTGT+GGG - chr4.3:70751904-70751923 None:intergenic 40.0%
TGAGGTGTAATAGAGAATGG+AGG - chr4.3:70751005-70751024 None:intergenic 40.0%
! AAAAATTGGGGACGGTGTTA+AGG + chr4.3:70752699-70752718 MS.gene77048:CDS 40.0%
! AAAATTGGGGACGGTGTTAA+GGG + chr4.3:70752700-70752719 MS.gene77048:CDS 40.0%
! AAGGGTGATTTGGGAGTTAA+TGG + chr4.3:70752829-70752848 MS.gene77048:CDS 40.0%
! CGTTGTTGAGGATTTTACGT+CGG + chr4.3:70752888-70752907 MS.gene77048:CDS 40.0%
! TAACGGTTCTTTAGCATCGA+TGG + chr4.3:70752085-70752104 MS.gene77048:CDS 40.0%
! TATGCGAAGTGCGTTCTTTT+TGG + chr4.3:70751666-70751685 MS.gene77048:CDS 40.0%
! TCAGAGATTGTTGCGTTGAT+CGG + chr4.3:70751633-70751652 MS.gene77048:CDS 40.0%
!! ACAAGGATAATGGAGACTGA+AGG + chr4.3:70752305-70752324 MS.gene77048:CDS 40.0%
!! CGTTTTACGTATAGTGGAAG+AGG - chr4.3:70751088-70751107 None:intergenic 40.0%
!! CTTTTAGAAAGCGGTTAGGT+AGG + chr4.3:70752426-70752445 MS.gene77048:CDS 40.0%
!! GACGTTGTTTCTGTTCTAGA+AGG + chr4.3:70751468-70751487 MS.gene77048:CDS 40.0%
!! GATGATGGTGGTTTAGTAGT+TGG - chr4.3:70750937-70750956 None:intergenic 40.0%
!! TTGGTTGAGTTGGCCAAAAA+TGG + chr4.3:70752470-70752489 MS.gene77048:CDS 40.0%
!!! GAGACTGAAGGTGTTTTAAG+TGG + chr4.3:70752317-70752336 MS.gene77048:CDS 40.0%
!!! GAGTGGTTGCTTTTAGAAAG+CGG + chr4.3:70752417-70752436 MS.gene77048:CDS 40.0%
!!! GTTGCTTTTAGAAAGCGGTT+AGG + chr4.3:70752422-70752441 MS.gene77048:CDS 40.0%
!!! TGAAGGTGTTTTAAGTGGTG+TGG + chr4.3:70752322-70752341 MS.gene77048:CDS 40.0%
!!! TGTGGCAGAGGTTTTAAGAT+CGG + chr4.3:70752340-70752359 MS.gene77048:CDS 40.0%
ACTGCATTAACCTGTAGGCT+CGG - chr4.3:70752245-70752264 None:intergenic 45.0%
AGGAAGACACGAATCGTTAG+TGG + chr4.3:70752446-70752465 MS.gene77048:CDS 45.0%
ATACGTAAAACGCACCTCCT+CGG + chr4.3:70751096-70751115 MS.gene77048:CDS 45.0%
ATGGCGTCGTTTATGAGCTT+CGG + chr4.3:70752117-70752136 MS.gene77048:CDS 45.0%
ATGGTGTGGAAAGGGTGATT+TGG + chr4.3:70752819-70752838 MS.gene77048:CDS 45.0%
ATTTGGGAGTTAATGGCGGT+GGG + chr4.3:70752836-70752855 MS.gene77048:CDS 45.0%
CACAACACGTGTCCTAAAAC+AGG + chr4.3:70751867-70751886 MS.gene77048:CDS 45.0%
GAAAGGTGGATCAGAGATTG+TGG + chr4.3:70752778-70752797 MS.gene77048:CDS 45.0%
GAATCAGTCGTTGGAATTGG+TGG + chr4.3:70751731-70751750 MS.gene77048:CDS 45.0%
GCTACCGTTTCACCTTCAAA+TGG - chr4.3:70751975-70751994 None:intergenic 45.0%
GGCAATTCATGGTGTGGAAA+GGG + chr4.3:70752811-70752830 MS.gene77048:CDS 45.0%
GGTGACAATACTTGAAGCAG+TGG + chr4.3:70752628-70752647 MS.gene77048:CDS 45.0%
GGTGTAATAGAGAATGGAGG+AGG - chr4.3:70751002-70751021 None:intergenic 45.0%
GGTTAGGTATTAACTCCGTG+TGG - chr4.3:70751905-70751924 None:intergenic 45.0%
GTAGCCGTTAAAGTTAACGG+TGG + chr4.3:70751990-70752009 MS.gene77048:CDS 45.0%
GTGACAATGTGTAGGAAAGG+TGG + chr4.3:70752764-70752783 MS.gene77048:CDS 45.0%
GTTCAAGACTTATCGGACAC+CGG - chr4.3:70751770-70751789 None:intergenic 45.0%
TCCTAAAACAGGTCAAACCC+TGG + chr4.3:70751878-70751897 MS.gene77048:CDS 45.0%
TGGTGTGGAAAGGGTGATTT+GGG + chr4.3:70752820-70752839 MS.gene77048:CDS 45.0%
TTGGCCAAAAATGGACCTGA+GGG + chr4.3:70752479-70752498 MS.gene77048:CDS 45.0%
! GACACGAATCGTTAGTGGAT+TGG + chr4.3:70752451-70752470 MS.gene77048:CDS 45.0%
! GAGGTGCGTTTTACGTATAG+TGG - chr4.3:70751094-70751113 None:intergenic 45.0%
! GCATCGATGGAAGAGACAAA+TGG + chr4.3:70752098-70752117 MS.gene77048:CDS 45.0%
! GGTGATTTGGGAGTTAATGG+CGG + chr4.3:70752832-70752851 MS.gene77048:CDS 45.0%
! GTTGAGGATTTTACGTCGGT+GGG + chr4.3:70752892-70752911 MS.gene77048:CDS 45.0%
! TGTTGAGGATTTTACGTCGG+TGG + chr4.3:70752891-70752910 MS.gene77048:CDS 45.0%
!! CGTTAGTGGATTGGTTGAGT+TGG + chr4.3:70752460-70752479 MS.gene77048:CDS 45.0%
!! CGTTCTTTTTGGTGCAACGA+CGG + chr4.3:70751677-70751696 MS.gene77048:CDS 45.0%
!! CGTTTATGAGCTTCGGGTTT+TGG + chr4.3:70752124-70752143 MS.gene77048:CDS 45.0%
!! TGTTTTAAGTGGTGTGGCAG+AGG + chr4.3:70752328-70752347 MS.gene77048:CDS 45.0%
!!! GTGGCAGAGGTTTTAAGATC+GGG + chr4.3:70752341-70752360 MS.gene77048:CDS 45.0%
AACGACGCCGTTTCTGATGA+TGG - chr4.3:70750952-70750971 None:intergenic 50.0%
ACACCATGGAGCTGCGTTTA+AGG - chr4.3:70751043-70751062 None:intergenic 50.0%
AGGTGGATCAGAGATTGTGG+CGG + chr4.3:70752781-70752800 MS.gene77048:CDS 50.0%
ATTTGCTTTAGCCTCCCACG+TGG - chr4.3:70752369-70752388 None:intergenic 50.0%
CGACGCTTGTGACAATGTGT+AGG + chr4.3:70752756-70752775 MS.gene77048:CDS 50.0%
CGAGCTTGTATAGCTGAAGC+TGG + chr4.3:70752167-70752186 MS.gene77048:CDS 50.0%
CGGCAATTCATGGTGTGGAA+AGG + chr4.3:70752810-70752829 MS.gene77048:CDS 50.0%
CGGGAAATGATGGCCGTTTA+CGG + chr4.3:70751706-70751725 MS.gene77048:CDS 50.0%
CTTGAAGCAGTGGTTAAGCG+TGG + chr4.3:70752638-70752657 MS.gene77048:CDS 50.0%
GAAATGATGGCCGTTTACGG+CGG + chr4.3:70751709-70751728 MS.gene77048:CDS 50.0%
GATTTGGGAGTTAATGGCGG+TGG + chr4.3:70752835-70752854 MS.gene77048:CDS 50.0%
GCGGAATCAGTCGTTGGAAT+TGG + chr4.3:70751728-70751747 MS.gene77048:CDS 50.0%
GGAAGCAACAAGAATGACGG+CGG + chr4.3:70752034-70752053 MS.gene77048:CDS 50.0%
GGTTAAGCGTGGAGGATTAG+TGG + chr4.3:70752649-70752668 MS.gene77048:CDS 50.0%
GTGGGAGGCTAAAGCAAATG+CGG + chr4.3:70752370-70752389 MS.gene77048:CDS 50.0%
GTTAAGGGAGGGATCAGAGA+GGG + chr4.3:70752715-70752734 MS.gene77048:CDS 50.0%
GTTCCTTAAACGCAGCTCCA+TGG + chr4.3:70751037-70751056 MS.gene77048:CDS 50.0%
GTTGGCCAAAAATGGACCTG+AGG + chr4.3:70752478-70752497 MS.gene77048:CDS 50.0%
TATTAACTCCGTGTGGGCCA+GGG - chr4.3:70751898-70751917 None:intergenic 50.0%
TGGCGTCGTTTATGAGCTTC+GGG + chr4.3:70752118-70752137 MS.gene77048:CDS 50.0%
TGTTAAGGGAGGGATCAGAG+AGG + chr4.3:70752714-70752733 MS.gene77048:CDS 50.0%
TTCGCAAAATCCGAGCCTAC+AGG + chr4.3:70752232-70752251 MS.gene77048:CDS 50.0%
TTTACGGCGGAATCAGTCGT+TGG + chr4.3:70751722-70751741 MS.gene77048:CDS 50.0%
TTTGCATCTCGCAGCGGATA+GGG + chr4.3:70752529-70752548 MS.gene77048:CDS 50.0%
TTTGGGAGTTAATGGCGGTG+GGG + chr4.3:70752837-70752856 MS.gene77048:CDS 50.0%
! ACACATTGTCACAAGCGTCG+CGG - chr4.3:70752756-70752775 None:intergenic 50.0%
! ACGACTGATTCCGCCGTAAA+CGG - chr4.3:70751722-70751741 None:intergenic 50.0%
! GCCAGGGTTTGACCTGTTTT+AGG - chr4.3:70751882-70751901 None:intergenic 50.0%
! GGCAATTTTGCATCTCGCAG+CGG + chr4.3:70752523-70752542 MS.gene77048:CDS 50.0%
! GGGTTTGGATGGTGGTGAAA+CGG + chr4.3:70752919-70752938 MS.gene77048:CDS 50.0%
! GTCGTTGGAATTGGTGGTTC+CGG + chr4.3:70751737-70751756 MS.gene77048:CDS 50.0%
! TAAGCGTGGAGGATTAGTGG+CGG + chr4.3:70752652-70752671 MS.gene77048:CDS 50.0%
! TTGGATGGTGGTGAAACGGA+GGG + chr4.3:70752923-70752942 MS.gene77048:CDS 50.0%
! TTTAAGATCGGGTGCCACGT+GGG + chr4.3:70752352-70752371 MS.gene77048:CDS 50.0%
! TTTGGATGGTGGTGAAACGG+AGG + chr4.3:70752922-70752941 MS.gene77048:CDS 50.0%
! TTTTGCATCTCGCAGCGGAT+AGG + chr4.3:70752528-70752547 MS.gene77048:CDS 50.0%
!! GCTTCGGGTTTTGGCGAAAT+CGG + chr4.3:70752133-70752152 MS.gene77048:CDS 50.0%
!! TGGGAAGGTTGGTAGAGAGT+GGG + chr4.3:70752558-70752577 MS.gene77048:CDS 50.0%
!!! TTTTAAGATCGGGTGCCACG+TGG + chr4.3:70752351-70752370 MS.gene77048:CDS 50.0%
AATCCCCGCTGTCATCTCCA+CGG - chr4.3:70752585-70752604 None:intergenic 55.0%
AGCGAAGAGGGACGCATTAG+CGG + chr4.3:70752502-70752521 MS.gene77048:CDS 55.0%
AGTATTGTCACCGCCTCCTC+CGG - chr4.3:70752620-70752639 None:intergenic 55.0%
CATCGAAAACGACACCGAGG+AGG - chr4.3:70751113-70751132 None:intergenic 55.0%
GAAGCAGTGGTTAAGCGTGG+AGG + chr4.3:70752641-70752660 MS.gene77048:CDS 55.0%
GAGTGTTAGAGGGAGGAGGA+AGG + chr4.3:70752859-70752878 MS.gene77048:CDS 55.0%
GATGACAGCGGGGATTATGG+CGG + chr4.3:70752589-70752608 MS.gene77048:CDS 55.0%
GATTATGGCGGTAATGCCGG+AGG + chr4.3:70752601-70752620 MS.gene77048:CDS 55.0%
GCTTGCGGCAATTCATGGTG+TGG + chr4.3:70752805-70752824 MS.gene77048:CDS 55.0%
GGAGATGACAGCGGGGATTA+TGG + chr4.3:70752586-70752605 MS.gene77048:CDS 55.0%
GGAGGCTAAAGCAAATGCGG+CGG + chr4.3:70752373-70752392 MS.gene77048:CDS 55.0%
GGGGATTATGGCGGTAATGC+CGG + chr4.3:70752598-70752617 MS.gene77048:CDS 55.0%
GGTCATCGAAAACGACACCG+AGG - chr4.3:70751116-70751135 None:intergenic 55.0%
GTATTAACTCCGTGTGGGCC+AGG - chr4.3:70751899-70751918 None:intergenic 55.0%
TGTTAGAGGGAGGAGGAAGG+CGG + chr4.3:70752862-70752881 MS.gene77048:CDS 55.0%
! AGAGATTGTGGCGGAGCTTG+CGG + chr4.3:70752790-70752809 MS.gene77048:CDS 55.0%
! ATTGGGGACGGTGTTAAGGG+AGG + chr4.3:70752703-70752722 MS.gene77048:CDS 55.0%
! CGCTCCCTCAGGTCCATTTT+TGG - chr4.3:70752486-70752505 None:intergenic 55.0%
! GAGGATTTTACGTCGGTGGG+CGG + chr4.3:70752895-70752914 MS.gene77048:CDS 55.0%
! GTTGGAATTGGTGGTTCCGG+CGG + chr4.3:70751740-70751759 MS.gene77048:CDS 55.0%
! TGGAGGATTAGTGGCGGTTG+CGG + chr4.3:70752658-70752677 MS.gene77048:CDS 55.0%
! TTGGGGACGGTGTTAAGGGA+GGG + chr4.3:70752704-70752723 MS.gene77048:CDS 55.0%
!! GACGCCGTTTCTGATGATGG+TGG - chr4.3:70750949-70750968 None:intergenic 55.0%
!! GGGAAGGTTGGTAGAGAGTG+GGG + chr4.3:70752559-70752578 MS.gene77048:CDS 55.0%
!! GTGGGAAGGTTGGTAGAGAG+TGG + chr4.3:70752557-70752576 MS.gene77048:CDS 55.0%
AGGTCAAACCCTGGCCCACA+CGG + chr4.3:70751887-70751906 MS.gene77048:CDS 60.0%
ATCGGACACCGGTAATCCGC+CGG - chr4.3:70751759-70751778 None:intergenic 60.0%
ATGGACCTGAGGGAGCGAAG+AGG + chr4.3:70752489-70752508 MS.gene77048:CDS 60.0%
GATCCTGTCGTCGTGGCAAC+AGG + chr4.3:70751798-70751817 MS.gene77048:CDS 60.0%
GCGGAGCTTGCGGCAATTCA+TGG + chr4.3:70752800-70752819 MS.gene77048:CDS 60.0%
TAGGGAGACGGTGGGAAGGT+TGG + chr4.3:70752547-70752566 MS.gene77048:CDS 60.0%
TATGGCGGTAATGCCGGAGG+AGG + chr4.3:70752604-70752623 MS.gene77048:CDS 60.0%
TCTCGCAGCGGATAGGGAGA+CGG + chr4.3:70752535-70752554 MS.gene77048:CDS 60.0%
TGGACCTGAGGGAGCGAAGA+GGG + chr4.3:70752490-70752509 MS.gene77048:CDS 60.0%
TGTCCTGTTGCCACGACGAC+AGG - chr4.3:70751804-70751823 None:intergenic 60.0%
! ATTTTACGTCGGTGGGCGGC+GGG + chr4.3:70752899-70752918 MS.gene77048:CDS 60.0%
! GATTTTACGTCGGTGGGCGG+CGG + chr4.3:70752898-70752917 MS.gene77048:CDS 60.0%
!! ACGGCGTCGACGGGAAATGA+TGG + chr4.3:70751696-70751715 MS.gene77048:CDS 60.0%
!! ATGGCGGTGGGGAGTGTTAG+AGG + chr4.3:70752848-70752867 MS.gene77048:CDS 60.0%
!! TGGCGGTGGGGAGTGTTAGA+GGG + chr4.3:70752849-70752868 MS.gene77048:CDS 60.0%
!! TGGGGAGTGTTAGAGGGAGG+AGG + chr4.3:70752855-70752874 MS.gene77048:CDS 60.0%
!! TGGTGGTTCCGGCGGATTAC+CGG + chr4.3:70751748-70751767 MS.gene77048:CDS 60.0%
AGAGAGGGCGAGGGAGAGTG+CGG + chr4.3:70752730-70752749 MS.gene77048:CDS 65.0%
CGGATAGGGAGACGGTGGGA+AGG + chr4.3:70752543-70752562 MS.gene77048:CDS 65.0%
CGGTTGCGGCAGCGTTTGTG+GGG + chr4.3:70752672-70752691 MS.gene77048:CDS 65.0%
GATGCGGGATCCTGTCGTCG+TGG + chr4.3:70751791-70751810 MS.gene77048:CDS 65.0%
GCAGCGGATAGGGAGACGGT+GGG + chr4.3:70752539-70752558 MS.gene77048:CDS 65.0%
GCGGTTGCGGCAGCGTTTGT+GGG + chr4.3:70752671-70752690 MS.gene77048:CDS 65.0%
GGAAGGCGGCGACGTTGTTG+AGG + chr4.3:70752876-70752895 MS.gene77048:CDS 65.0%
GGAGGGATCAGAGAGGGCGA+GGG + chr4.3:70752721-70752740 MS.gene77048:CDS 65.0%
TGGGGCCGTGGAGATGACAG+CGG + chr4.3:70752577-70752596 MS.gene77048:CDS 65.0%
! AAGATCGGGTGCCACGTGGG+AGG + chr4.3:70752355-70752374 MS.gene77048:CDS 65.0%
! GTTGGTAGAGAGTGGGGCCG+TGG + chr4.3:70752565-70752584 MS.gene77048:CDS 65.0%
! TGGTGCAACGACGGCGTCGA+CGG + chr4.3:70751686-70751705 MS.gene77048:CDS 65.0%
!! CGGTGGGGAGTGTTAGAGGG+AGG + chr4.3:70752852-70752871 MS.gene77048:CDS 65.0%
CGCAGCGGATAGGGAGACGG+TGG + chr4.3:70752538-70752557 MS.gene77048:CDS 70.0%
GCGTCCCTCTTCGCTCCCTC+AGG - chr4.3:70752497-70752516 None:intergenic 70.0%
GGCGGTAATGCCGGAGGAGG+CGG + chr4.3:70752607-70752626 MS.gene77048:CDS 70.0%
GGCGGTTGCGGCAGCGTTTG+TGG + chr4.3:70752670-70752689 MS.gene77048:CDS 70.0%
GGGAGGGATCAGAGAGGGCG+AGG + chr4.3:70752720-70752739 MS.gene77048:CDS 70.0%
GGGCCGTGGAGATGACAGCG+GGG + chr4.3:70752579-70752598 MS.gene77048:CDS 70.0%
GGGGCCGTGGAGATGACAGC+GGG + chr4.3:70752578-70752597 MS.gene77048:CDS 70.0%
! TGGGCGGCGGGTTTGGATGG+TGG + chr4.3:70752911-70752930 MS.gene77048:CDS 70.0%
!! GGTGCAACGACGGCGTCGAC+GGG + chr4.3:70751687-70751706 MS.gene77048:CDS 70.0%
! ACGTCGGTGGGCGGCGGGTT+TGG + chr4.3:70752904-70752923 MS.gene77048:CDS 75.0%
! CGGTGGGCGGCGGGTTTGGA+TGG + chr4.3:70752908-70752927 MS.gene77048:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 70750910 70753021 70750910 ID=MS.gene77048
chr4.3 mRNA 70750910 70753021 70750910 ID=MS.gene77048.t1;Parent=MS.gene77048
chr4.3 exon 70750910 70753021 70750910 ID=MS.gene77048.t1.exon1;Parent=MS.gene77048.t1
chr4.3 CDS 70750910 70753021 70750910 ID=cds.MS.gene77048.t1;Parent=MS.gene77048.t1
Gene Sequence

>MS.gene77048

ATGGCAATATCACCTCAAACATTTCCAACTACTAAACCACCATCATCAGAAACGGCGTCGTTTATCACCCCAAATATCACTAACTTTAACCTCCTCCATTCTCTATTACACCTCACAAACCAAATATGTTCCTTAAACGCAGCTCCATGGTGTCGTTTCCTTTTAAACAAAATTTCCTCTTCCACTATACGTAAAACGCACCTCCTCGGTGTCGTTTTCGATGACCTAATTCGTAATTCAATATCAGTTTCAGCTTCGAATTCAACTCGTCAAAATTCTTACGTTCTTTGTTTAGAAGAAATGTATATTGTGCTTCATAGGATTAAAACTCTGCTCGAAGATTGTTCTAATGGAAGCAAATTTTCTATTCTTATGCAGATTGAAACTGTTGCTGATAATTTCAACAAATTAACCGGTGAATTATCAACTCTTCTTGATGTTTTTCCAATTCAAGAAATGAACTTGAATGATGATGTATTGGAGCTTGTTCTTCTGATTAGAAAACAGTGTTCAGAAACGAAGAATTTGTTAGGTGAAGAACAAATTCATCTCCGAAACGACGTTGTTTCTGTTCTAGAAGGAATCAAGAGAGAAATGGTTCCAAAAGATGAAAAATTATCTTCAATTTTTGAGAAATTGGAGATTCAAGATTCTTCAAGTTGTAGAAGTGAGATTGAAAATCTTCAAGAAGAAATTCAGAATCGGAGCGAAGAACAATCGAAATCAGAGATTGTTGCGTTGATCGGACTTGTTCGTTATGCGAAGTGCGTTCTTTTTGGTGCAACGACGGCGTCGACGGGAAATGATGGCCGTTTACGGCGGAATCAGTCGTTGGAATTGGTGGTTCCGGCGGATTACCGGTGTCCGATAAGTCTTGAACTGATGCGGGATCCTGTCGTCGTGGCAACAGGACAGACGTACGATCGTGTTTCGATTAAGCTTTGGATGGATTCTGGGCACAACACGTGTCCTAAAACAGGTCAAACCCTGGCCCACACGGAGTTAATACCTAACCATGCTTTGAGAAATATGATAGCTATTTGGTGCAAAGAACAGAAAATTCCATTTGAAGGTGAAACGGTAGCCGTTAAAGTTAACGGTGGTATGACAAATAAGACAGCTTTGGAAGCAACAAGAATGACGGCGGCGTTTTTAGTCAACAAGTTGACGTTGACTAACGGTTCTTTAGCATCGATGGAAGAGACAAATGGCGTCGTTTATGAGCTTCGGGTTTTGGCGAAATCGGATTCAGAAAGTCGAGCTTGTATAGCTGAAGCTGGAGCTATAGAGATTTTGATTCGGTTTTTAAGTTATGAAGTTGGTTCGCAAAATCCGAGCCTACAGGTTAATGCAGTTACAACGATATTAAATCTCTCGATACTTGAAGCGAATAAGACAAGGATAATGGAGACTGAAGGTGTTTTAAGTGGTGTGGCAGAGGTTTTAAGATCGGGTGCCACGTGGGAGGCTAAAGCAAATGCGGCGGCTACTGTGTTTAGTTTAACTGGAGTGGTTGCTTTTAGAAAGCGGTTAGGTAGGAAGACACGAATCGTTAGTGGATTGGTTGAGTTGGCCAAAAATGGACCTGAGGGAGCGAAGAGGGACGCATTAGCGGCAATTTTGCATCTCGCAGCGGATAGGGAGACGGTGGGAAGGTTGGTAGAGAGTGGGGCCGTGGAGATGACAGCGGGGATTATGGCGGTAATGCCGGAGGAGGCGGTGACAATACTTGAAGCAGTGGTTAAGCGTGGAGGATTAGTGGCGGTTGCGGCAGCGTTTGTGGGGATAAAAAAATTGGGGACGGTGTTAAGGGAGGGATCAGAGAGGGCGAGGGAGAGTGCGGCCGCGACGCTTGTGACAATGTGTAGGAAAGGTGGATCAGAGATTGTGGCGGAGCTTGCGGCAATTCATGGTGTGGAAAGGGTGATTTGGGAGTTAATGGCGGTGGGGAGTGTTAGAGGGAGGAGGAAGGCGGCGACGTTGTTGAGGATTTTACGTCGGTGGGCGGCGGGTTTGGATGGTGGTGAAACGGAGGGATTTACATCAACGAACACTTTTGTTAGTACATCAACTACCATGGTTGTTCCAACTACAACTTCATTAGCACAGTAA
Protein sequence

>MS.gene77048.t1

MAISPQTFPTTKPPSSETASFITPNITNFNLLHSLLHLTNQICSLNAAPWCRFLLNKISSSTIRKTHLLGVVFDDLIRNSISVSASNSTRQNSYVLCLEEMYIVLHRIKTLLEDCSNGSKFSILMQIETVADNFNKLTGELSTLLDVFPIQEMNLNDDVLELVLLIRKQCSETKNLLGEEQIHLRNDVVSVLEGIKREMVPKDEKLSSIFEKLEIQDSSSCRSEIENLQEEIQNRSEEQSKSEIVALIGLVRYAKCVLFGATTASTGNDGRLRRNQSLELVVPADYRCPISLELMRDPVVVATGQTYDRVSIKLWMDSGHNTCPKTGQTLAHTELIPNHALRNMIAIWCKEQKIPFEGETVAVKVNGGMTNKTALEATRMTAAFLVNKLTLTNGSLASMEETNGVVYELRVLAKSDSESRACIAEAGAIEILIRFLSYEVGSQNPSLQVNAVTTILNLSILEANKTRIMETEGVLSGVAEVLRSGATWEAKANAAATVFSLTGVVAFRKRLGRKTRIVSGLVELAKNGPEGAKRDALAAILHLAADRETVGRLVESGAVEMTAGIMAVMPEEAVTILEAVVKRGGLVAVAAAFVGIKKLGTVLREGSERARESAAATLVTMCRKGGSEIVAELAAIHGVERVIWELMAVGSVRGRRKAATLLRILRRWAAGLDGGETEGFTSTNTFVSTSTTMVVPTTTSLAQ