AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77102

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77102.t1 MTR_6g047800 98.000 300 5 1 1 299 10 309 0.0 607
MS.gene77102.t1 MTR_6g047800 98.000 300 5 1 1 299 1 300 0.0 606
MS.gene77102.t1 MTR_2g014590 79.730 222 45 0 1 222 11 232 1.10e-135 384
MS.gene77102.t1 MTR_2g014570 70.968 62 13 3 237 298 6 62 6.00e-19 79.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77102.t1 AT5G14600 75.082 305 69 2 2 299 11 315 7.84e-173 481

Find 70 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTTTGTTGTTATGTATT+TGG 0.132518 2.4:+32651831 MS.gene77102:CDS
TCCTAATTCTTCTTCTGTTA+TGG 0.259735 2.4:+32653876 MS.gene77102:CDS
AACAATTAGTAACGGTGATT+TGG 0.271982 2.4:+32651582 MS.gene77102:CDS
TCCTGAAGAATTTGCTGGCT+TGG 0.291582 2.4:+32653423 MS.gene77102:CDS
CGAAGTTCGAGAAGGGAAAA+TGG 0.297664 2.4:+32653762 MS.gene77102:CDS
TTGTTTATGTGAAAGATATA+AGG 0.312566 2.4:+32653713 MS.gene77102:intron
GGTTGTGTCGTTCTTGAATC+TGG 0.320717 2.4:+32651865 MS.gene77102:CDS
AAAACTAAAGTCCACAATTC+AGG 0.328439 2.4:-32651772 None:intergenic
GATTGGATAGGAAAGTCATT+TGG 0.331486 2.4:+32651697 MS.gene77102:CDS
GTCTAAAGTATTCAGCAATA+AGG 0.337791 2.4:+32651720 MS.gene77102:CDS
GCCAAGCCAGCAAATTCTTC+AGG 0.357310 2.4:-32653424 None:intergenic
GCCATAACAGAAGAAGAATT+AGG 0.368158 2.4:-32653877 None:intergenic
GGGTTTCCTGAAGAATTTGC+TGG 0.381718 2.4:+32653418 MS.gene77102:CDS
ATGTATTTGGAGATTGTTCC+TGG 0.385811 2.4:+32651844 MS.gene77102:CDS
AGGGATGACTTTGAGAAGAT+TGG 0.392112 2.4:+32653349 MS.gene77102:intron
AACGGTGATGACGGCTCTAA+TGG 0.392261 2.4:+32653799 MS.gene77102:CDS
CTTGAATCTGGTACTGGAAG+TGG 0.400748 2.4:+32651877 MS.gene77102:CDS
GATCCCTCAGATACCGTTAC+TGG 0.416695 2.4:-32651637 None:intergenic
ATTGGATAGGAAAGTCATTT+GGG 0.422230 2.4:+32651698 MS.gene77102:CDS
ATGGAAAGCTTAGAGATTAA+CGG 0.454257 2.4:+32653781 MS.gene77102:CDS
TCTAAAGTATTCAGCAATAA+GGG 0.459899 2.4:+32651721 MS.gene77102:CDS
TTGCAAGAGGAGGCAACGTT+CGG 0.460487 2.4:+32653831 MS.gene77102:CDS
GTCGTTCTTGAATCTGGTAC+TGG 0.478619 2.4:+32651871 MS.gene77102:CDS
CTTTAACTACTTCACTTGCA+AGG 0.505822 2.4:+32651902 MS.gene77102:CDS
ACGGGAAGTACCAGTGAAGT+TGG 0.515604 2.4:-32653584 None:intergenic
GCAAGGGCAGTTGCTCCTAC+AGG 0.518867 2.4:+32651919 MS.gene77102:CDS
ACAATGCAATCCAACTTCAC+TGG 0.520816 2.4:+32653574 MS.gene77102:CDS
TTGAATCTGGTACTGGAAGT+GGG 0.536848 2.4:+32651878 MS.gene77102:CDS
CGTTGTACTTGTTCAATACA+CGG 0.539892 2.4:-32653541 None:intergenic
ATTTGGCAATTGTTTATGCA+AGG 0.545176 2.4:+32651599 MS.gene77102:CDS
ATAAGCAGCTTAGTCACTGC+GGG 0.545723 2.4:+32653373 MS.gene77102:CDS
GCGGGAGTGAGGGACATTCA+AGG 0.551907 2.4:+32653391 MS.gene77102:CDS
GCTTAGTCACTGCGGGAGTG+AGG 0.551945 2.4:+32653380 MS.gene77102:CDS
TTCAAACATTCAGATTGGAT+AGG 0.552647 2.4:+32651685 MS.gene77102:CDS
GAACGTTGCCTCCTCTTGCA+AGG 0.566361 2.4:-32653829 None:intergenic
GAAGCCAGTAACGGTATCTG+AGG 0.567229 2.4:+32651633 MS.gene77102:CDS
CTTAGTCACTGCGGGAGTGA+GGG 0.567587 2.4:+32653381 MS.gene77102:CDS
GGAATAGCTAGCCAAGGTTG+AGG 0.572196 2.4:-32653469 None:intergenic
TAGCACCTACTCCTGAATTG+TGG 0.573848 2.4:+32651761 MS.gene77102:CDS
ATGGTTCTCTTCCTTGCAAG+AGG 0.575002 2.4:+32653818 MS.gene77102:CDS
TTTAACTACTTCACTTGCAA+GGG 0.578833 2.4:+32651903 MS.gene77102:CDS
GTGAGGGACATTCAAGGTGA+GGG 0.582946 2.4:+32653397 MS.gene77102:CDS
GTTCTCTTCCTTGCAAGAGG+AGG 0.584259 2.4:+32653821 MS.gene77102:CDS
GTTGTACTTGTTCAATACAC+GGG 0.584848 2.4:-32653540 None:intergenic
GATTCAAGAACGACACAACC+AGG 0.585429 2.4:-32651862 None:intergenic
AGTAACGGTATCTGAGGGAT+CGG 0.588437 2.4:+32651639 MS.gene77102:CDS
TTAGAGATTAACGGTGATGA+CGG 0.592052 2.4:+32653790 MS.gene77102:CDS
AAAGTCCACAATTCAGGAGT+AGG 0.594923 2.4:-32651766 None:intergenic
TTTAATAGAACAATTAGTAA+CGG 0.596180 2.4:+32651574 MS.gene77102:CDS
AACGTATAAACATGTCCTGT+AGG 0.611682 2.4:-32651934 None:intergenic
CGGACATACGAAGTTCGAGA+AGG 0.611846 2.4:+32653754 MS.gene77102:CDS
CTCGAACTTCGTATGTCCGT+AGG 0.612530 2.4:-32653750 None:intergenic
TGACCTCTAGCTTCACCACA+TGG 0.618356 2.4:-32653904 None:intergenic
AAGAGGAGGCAACGTTCGGA+TGG 0.621142 2.4:+32653835 MS.gene77102:CDS
GGACATACGAAGTTCGAGAA+GGG 0.625372 2.4:+32653755 MS.gene77102:CDS
TGAGGGACATTCAAGGTGAG+GGG 0.627956 2.4:+32653398 MS.gene77102:CDS
TCTGTTATGGCTAGACCATG+TGG 0.628693 2.4:+32653889 MS.gene77102:CDS
AACAGAAGAAGAATTAGGAT+AGG 0.630818 2.4:-32653872 None:intergenic
ACGTTCGGATGGAGCTAGTG+TGG 0.632897 2.4:+32653846 MS.gene77102:CDS
AACAAAGATCTGGATCAGCT+AGG 0.636973 2.4:+32651968 MS.gene77102:CDS
AGACCATGTGGTGAAGCTAG+AGG 0.638065 2.4:+32653901 MS.gene77102:CDS
AGTGAGGGACATTCAAGGTG+AGG 0.642071 2.4:+32653396 MS.gene77102:CDS
GCTGAAGGAATAGCTAGCCA+AGG 0.647664 2.4:-32653475 None:intergenic
CATATAGAATCTGTGTCCTG+TGG 0.649958 2.4:-32651798 None:intergenic
AAGCCAGTAACGGTATCTGA+GGG 0.657172 2.4:+32651634 MS.gene77102:CDS
TGACAACATGAAGCCAGTAA+CGG 0.669534 2.4:+32651624 MS.gene77102:CDS
GGTGAAGCTAGAGGTCACAC+TGG 0.683965 2.4:+32653910 MS.gene77102:CDS
AAAGTATTCAGCAATAAGGG+CGG 0.684692 2.4:+32651724 MS.gene77102:CDS
CATAAGCAGCTTAGTCACTG+CGG 0.689323 2.4:+32653372 MS.gene77102:CDS
TTGTACTTGTTCAATACACG+GGG 0.704021 2.4:-32653539 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATATAAAAGAAGAAAAAA+CGG - chr2.4:32653606-32653625 None:intergenic 15.0%
!! AGTCAATTATATATAAAACA+AGG - chr2.4:32653043-32653062 None:intergenic 15.0%
!! ATAGCTTATATATTGTATTA+CGG - chr2.4:32652981-32653000 None:intergenic 15.0%
!! TTTAATAGAACAATTAGTAA+CGG + chr2.4:32651574-32651593 MS.gene77102:CDS 15.0%
!! ATAATATTACTTGCAATTAC+TGG - chr2.4:32653193-32653212 None:intergenic 20.0%
!! CATATAAAAGAAGAAAAAAC+GGG - chr2.4:32653605-32653624 None:intergenic 20.0%
!! GTAATTGCAAGTAATATTAT+TGG + chr2.4:32653193-32653212 MS.gene77102:intron 20.0%
!! TTGTTTATGTGAAAGATATA+AGG + chr2.4:32653713-32653732 MS.gene77102:intron 20.0%
!!! AATATTGTTTTTCTTCTTGT+AGG + chr2.4:32653329-32653348 MS.gene77102:intron 20.0%
!!! ATATTGTTTTTCTTCTTGTA+GGG + chr2.4:32653330-32653349 MS.gene77102:intron 20.0%
!!! TTTTTACAATGGAAAAACTT+AGG + chr2.4:32652928-32652947 MS.gene77102:intron 20.0%
! ACATAAACAAACGTATACAA+AGG - chr2.4:32653703-32653722 None:intergenic 25.0%
! ACCCTACATACAAATAAAAA+AGG - chr2.4:32653019-32653038 None:intergenic 25.0%
! ATATACATGTGCTTATCTTT+AGG + chr2.4:32652025-32652044 MS.gene77102:intron 25.0%
! CATACAAATAAAAAAGGGAA+TGG - chr2.4:32653013-32653032 None:intergenic 25.0%
! CCCTACATACAAATAAAAAA+GGG - chr2.4:32653018-32653037 None:intergenic 25.0%
! CTACCAAAAAACTAGAATAA+CGG - chr2.4:32652154-32652173 None:intergenic 25.0%
! TGAAATAGAAGATAGTAACA+CGG - chr2.4:32653099-32653118 None:intergenic 25.0%
! TTATTTAACTGTACTCAATG+CGG + chr2.4:32653262-32653281 MS.gene77102:intron 25.0%
!! TCTAAAGTATTCAGCAATAA+GGG + chr2.4:32651721-32651740 MS.gene77102:CDS 25.0%
!! TTGTCAAATTGATTCAATAG+CGG + chr2.4:32652711-32652730 MS.gene77102:intron 25.0%
!! TTTCTAGTGTTGTCAAATAT+TGG + chr2.4:32652066-32652085 MS.gene77102:intron 25.0%
!!! CCCTTTTTTATTTGTATGTA+GGG + chr2.4:32653015-32653034 MS.gene77102:intron 25.0%
!!! TCACCAAAAATACGTTTTTT+AGG - chr2.4:32652271-32652290 None:intergenic 25.0%
!!! TCCCTTTTTTATTTGTATGT+AGG + chr2.4:32653014-32653033 MS.gene77102:intron 25.0%
AAAACTAAAGTCCACAATTC+AGG - chr2.4:32651775-32651794 None:intergenic 30.0%
AACAATTAGTAACGGTGATT+TGG + chr2.4:32651582-32651601 MS.gene77102:CDS 30.0%
AACAGAAGAAGAATTAGGAT+AGG - chr2.4:32653875-32653894 None:intergenic 30.0%
ACAAATAAAAAAGGGAATGG+AGG - chr2.4:32653010-32653029 None:intergenic 30.0%
ATGGAAAGCTTAGAGATTAA+CGG + chr2.4:32653781-32653800 MS.gene77102:CDS 30.0%
ATTGGATAGGAAAGTCATTT+GGG + chr2.4:32651698-32651717 MS.gene77102:CDS 30.0%
ATTGTAAAAATAAGCCACGT+TGG - chr2.4:32652918-32652937 None:intergenic 30.0%
ATTTGGCAATTGTTTATGCA+AGG + chr2.4:32651599-32651618 MS.gene77102:CDS 30.0%
CTATCTTCTATTTCACATAG+CGG + chr2.4:32653104-32653123 MS.gene77102:intron 30.0%
GAAATAGAAGATAGTAACAC+GGG - chr2.4:32653098-32653117 None:intergenic 30.0%
GACTTATTTCAACTGTTATG+CGG + chr2.4:32653059-32653078 MS.gene77102:intron 30.0%
TATTGTATTACGGTGAATAC+AGG - chr2.4:32652971-32652990 None:intergenic 30.0%
TCCTAATTCTTCTTCTGTTA+TGG + chr2.4:32653876-32653895 MS.gene77102:CDS 30.0%
TTCAAACATTCAGATTGGAT+AGG + chr2.4:32651685-32651704 MS.gene77102:CDS 30.0%
TTGTAAAAATAAGCCACGTT+GGG - chr2.4:32652917-32652936 None:intergenic 30.0%
TTTAACTACTTCACTTGCAA+GGG + chr2.4:32651903-32651922 MS.gene77102:CDS 30.0%
TTTCCGTAATATGCTATTGA+TGG + chr2.4:32653128-32653147 MS.gene77102:intron 30.0%
! AGCAGAAAAAAGAGACTTTT+AGG + chr2.4:32652190-32652209 MS.gene77102:intron 30.0%
! ATTTGATTTCCATGAGCATA+GGG + chr2.4:32652597-32652616 MS.gene77102:intron 30.0%
! GAAAGATATAAGGACTTTTG+AGG + chr2.4:32653723-32653742 MS.gene77102:intron 30.0%
! GTCTAAAGTATTCAGCAATA+AGG + chr2.4:32651720-32651739 MS.gene77102:CDS 30.0%
! GTGTTTTCAAACATTCAGAT+TGG + chr2.4:32651680-32651699 MS.gene77102:CDS 30.0%
!! ATCTTGCAATTCATTGATTC+AGG - chr2.4:32652343-32652362 None:intergenic 30.0%
!! CAGCTTTGTTGTTATGTATT+TGG + chr2.4:32651831-32651850 MS.gene77102:CDS 30.0%
!!! AAACGTATTTTTGGTGAAAC+TGG + chr2.4:32652274-32652293 MS.gene77102:intron 30.0%
!!! AACGTATTTTTGGTGAAACT+GGG + chr2.4:32652275-32652294 MS.gene77102:intron 30.0%
!!! AACGTGGCTTATTTTTACAA+TGG + chr2.4:32652917-32652936 MS.gene77102:intron 30.0%
!!! CCAAAAATACGTTTTTTAGG+CGG - chr2.4:32652268-32652287 None:intergenic 30.0%
!!! TAGTGTTGCAGTTTTTTTAG+CGG - chr2.4:32652120-32652139 None:intergenic 30.0%
!!! TTGCCGTTATTCTAGTTTTT+TGG + chr2.4:32652148-32652167 MS.gene77102:intron 30.0%
AACGTATAAACATGTCCTGT+AGG - chr2.4:32651937-32651956 None:intergenic 35.0%
ACACATGTATCAAACATCAG+AGG - chr2.4:32652652-32652671 None:intergenic 35.0%
ACTTGAGATGTTGAGTGTAA+AGG + chr2.4:32653291-32653310 MS.gene77102:intron 35.0%
ATAAACATGCCCTTCACAAA+TGG - chr2.4:32652575-32652594 None:intergenic 35.0%
ATCCCTTCATAAATTGGCTA+TGG + chr2.4:32652509-32652528 MS.gene77102:intron 35.0%
ATGTATTTGGAGATTGTTCC+TGG + chr2.4:32651844-32651863 MS.gene77102:CDS 35.0%
CAATGGAAAAACTTAGGAGT+CGG + chr2.4:32652934-32652953 MS.gene77102:intron 35.0%
CGTAGTAGTTATGCAACTAA+TGG + chr2.4:32653650-32653669 MS.gene77102:intron 35.0%
CGTTGTACTTGTTCAATACA+CGG - chr2.4:32653544-32653563 None:intergenic 35.0%
CTTTAACTACTTCACTTGCA+AGG + chr2.4:32651902-32651921 MS.gene77102:CDS 35.0%
GATTGGATAGGAAAGTCATT+TGG + chr2.4:32651697-32651716 MS.gene77102:CDS 35.0%
GCAAAAATGTTACGACATGA+CGG + chr2.4:32653499-32653518 MS.gene77102:CDS 35.0%
GCCATAACAGAAGAAGAATT+AGG - chr2.4:32653880-32653899 None:intergenic 35.0%
GGTTATCTTCCTTGAAATTC+CGG - chr2.4:32652322-32652341 None:intergenic 35.0%
GTGCCATCAATAGCATATTA+CGG - chr2.4:32653134-32653153 None:intergenic 35.0%
GTTATCTTCCTTGAAATTCC+GGG - chr2.4:32652321-32652340 None:intergenic 35.0%
GTTGTACTTGTTCAATACAC+GGG - chr2.4:32653543-32653562 None:intergenic 35.0%
TTAGAGATTAACGGTGATGA+CGG + chr2.4:32653790-32653809 MS.gene77102:CDS 35.0%
TTGTACTTGTTCAATACACG+GGG - chr2.4:32653542-32653561 None:intergenic 35.0%
! AAAGTATTCAGCAATAAGGG+CGG + chr2.4:32651724-32651743 MS.gene77102:CDS 35.0%
! CATTTGATTTCCATGAGCAT+AGG + chr2.4:32652596-32652615 MS.gene77102:intron 35.0%
! CCGCCTAAAAAACGTATTTT+TGG + chr2.4:32652265-32652284 MS.gene77102:intron 35.0%
! GATTTTCACGAACAAAGATC+TGG + chr2.4:32651958-32651977 MS.gene77102:CDS 35.0%
! TCGGTTTTACAGAATCGATT+CGG + chr2.4:32651658-32651677 MS.gene77102:CDS 35.0%
!!! ATACGTTTTTTAGGCGGTAA+TGG - chr2.4:32652262-32652281 None:intergenic 35.0%
AAAGTCCACAATTCAGGAGT+AGG - chr2.4:32651769-32651788 None:intergenic 40.0%
AACAAAGATCTGGATCAGCT+AGG + chr2.4:32651968-32651987 MS.gene77102:CDS 40.0%
ACAATGCAATCCAACTTCAC+TGG + chr2.4:32653574-32653593 MS.gene77102:CDS 40.0%
ACCGCAGAAAATTGTAGGTA+CGG - chr2.4:32652370-32652389 None:intergenic 40.0%
ACTATTGTGCTATCACATGG+TGG + chr2.4:32652735-32652754 MS.gene77102:intron 40.0%
ATATTGGCGCTATAGCATAG+CGG + chr2.4:32652082-32652101 MS.gene77102:intron 40.0%
ATCAAACATCAGAGGAAGTC+TGG - chr2.4:32652644-32652663 None:intergenic 40.0%
CATATAGAATCTGTGTCCTG+TGG - chr2.4:32651801-32651820 None:intergenic 40.0%
CCTTCATAAATTGGCTATGG+CGG + chr2.4:32652512-32652531 MS.gene77102:intron 40.0%
CGCCATAGCCAATTTATGAA+GGG - chr2.4:32652514-32652533 None:intergenic 40.0%
CTTCATAAATTGGCTATGGC+GGG + chr2.4:32652513-32652532 MS.gene77102:intron 40.0%
GTATGGTCGGAATCAGAATT+TGG - chr2.4:32652410-32652429 None:intergenic 40.0%
TGACAACATGAAGCCAGTAA+CGG + chr2.4:32651624-32651643 MS.gene77102:CDS 40.0%
TTAGGCTGTGTTGTCAATTG+TGG + chr2.4:32652208-32652227 MS.gene77102:intron 40.0%
! AGGGATGACTTTGAGAAGAT+TGG + chr2.4:32653349-32653368 MS.gene77102:intron 40.0%
! CGTAACATTTTTGCAGCTGA+AGG - chr2.4:32653493-32653512 None:intergenic 40.0%
! TGCTATTGATGGCACTTTCA+TGG + chr2.4:32653139-32653158 MS.gene77102:intron 40.0%
! TTGAATCTGGTACTGGAAGT+GGG + chr2.4:32651878-32651897 MS.gene77102:CDS 40.0%
! TTTTGGATCTACCTCAACCT+TGG + chr2.4:32653458-32653477 MS.gene77102:CDS 40.0%
!!! CTTGGCTGATGCAGTTTTTT+TGG + chr2.4:32653441-32653460 MS.gene77102:CDS 40.0%
!!! GATCGTGATTTTGACTTCCT+TGG + chr2.4:32652475-32652494 MS.gene77102:intron 40.0%
!!! GGACTTTAGTTTTAAGCCAC+AGG + chr2.4:32651782-32651801 MS.gene77102:CDS 40.0%
AACATGCCCTTCACAAATGG+TGG - chr2.4:32652572-32652591 None:intergenic 45.0%
AAGCCAGTAACGGTATCTGA+GGG + chr2.4:32651634-32651653 MS.gene77102:CDS 45.0%
AGTAACGGTATCTGAGGGAT+CGG + chr2.4:32651639-32651658 MS.gene77102:CDS 45.0%
ATAAGCAGCTTAGTCACTGC+GGG + chr2.4:32653373-32653392 MS.gene77102:CDS 45.0%
ATGATCCCCTATGAAAACCG+CGG + chr2.4:32652764-32652783 MS.gene77102:intron 45.0%
ATGGTTCTCTTCCTTGCAAG+AGG + chr2.4:32653818-32653837 MS.gene77102:CDS 45.0%
CATAAGCAGCTTAGTCACTG+CGG + chr2.4:32653372-32653391 MS.gene77102:CDS 45.0%
CCGCCATAGCCAATTTATGA+AGG - chr2.4:32652515-32652534 None:intergenic 45.0%
CGAAGTTCGAGAAGGGAAAA+TGG + chr2.4:32653762-32653781 MS.gene77102:CDS 45.0%
CTATCCCAGTTTCGCTATCA+GGG + chr2.4:32652789-32652808 MS.gene77102:intron 45.0%
GATTCAAGAACGACACAACC+AGG - chr2.4:32651865-32651884 None:intergenic 45.0%
GCAGAAAATTGTAGGTACGG+CGG - chr2.4:32652367-32652386 None:intergenic 45.0%
GCAGGCATCCCTTCATAAAT+TGG + chr2.4:32652503-32652522 MS.gene77102:intron 45.0%
GCATCACCGCAGAAAATTGT+AGG - chr2.4:32652375-32652394 None:intergenic 45.0%
GCTGTGTTGTCAATTGTGGA+AGG + chr2.4:32652212-32652231 MS.gene77102:intron 45.0%
GGACATACGAAGTTCGAGAA+GGG + chr2.4:32653755-32653774 MS.gene77102:CDS 45.0%
GGCACTATTGTGCTATCACA+TGG + chr2.4:32652732-32652751 MS.gene77102:intron 45.0%
GGGTTTCCTGAAGAATTTGC+TGG + chr2.4:32653418-32653437 MS.gene77102:CDS 45.0%
GGTCGGAATCAGAATTTGGT+GGG - chr2.4:32652406-32652425 None:intergenic 45.0%
GTCGTTCTTGAATCTGGTAC+TGG + chr2.4:32651871-32651890 MS.gene77102:CDS 45.0%
TAGCACCTACTCCTGAATTG+TGG + chr2.4:32651761-32651780 MS.gene77102:CDS 45.0%
TCTGTTATGGCTAGACCATG+TGG + chr2.4:32653889-32653908 MS.gene77102:CDS 45.0%
TGGTCGGAATCAGAATTTGG+TGG - chr2.4:32652407-32652426 None:intergenic 45.0%
TTTCGCTATCAGGGCAATAG+CGG + chr2.4:32652798-32652817 MS.gene77102:intron 45.0%
! ACAATTTTCTGCGGTGATGC+TGG + chr2.4:32652375-32652394 MS.gene77102:intron 45.0%
! ATTGCCCTGATAGCGAAACT+GGG - chr2.4:32652796-32652815 None:intergenic 45.0%
! CGTATGATTTTGCTACCCCT+TGG - chr2.4:32652442-32652461 None:intergenic 45.0%
! CTTGAATCTGGTACTGGAAG+TGG + chr2.4:32651877-32651896 MS.gene77102:CDS 45.0%
! GAATCAGAATTTGGTGGGTG+CGG - chr2.4:32652401-32652420 None:intergenic 45.0%
! GCCGTACCTACAATTTTCTG+CGG + chr2.4:32652366-32652385 MS.gene77102:intron 45.0%
! GGTTGTGTCGTTCTTGAATC+TGG + chr2.4:32651865-32651884 MS.gene77102:CDS 45.0%
! TATTGCCCTGATAGCGAAAC+TGG - chr2.4:32652797-32652816 None:intergenic 45.0%
!! TCCTGAAGAATTTGCTGGCT+TGG + chr2.4:32653423-32653442 MS.gene77102:CDS 45.0%
!! TTTTTGGTGAAACTGGGTCC+TGG + chr2.4:32652281-32652300 MS.gene77102:intron 45.0%
!!! GATTTTGACTTCCTTGGCCA+TGG + chr2.4:32652481-32652500 MS.gene77102:intron 45.0%
!!! GGACTTTTGAGGTACTCCTA+CGG + chr2.4:32653734-32653753 MS.gene77102:CDS 45.0%
ACCATACTCCAGCATGCCAA+GGG + chr2.4:32652423-32652442 MS.gene77102:intron 50.0%
AGACCATGTGGTGAAGCTAG+AGG + chr2.4:32653901-32653920 MS.gene77102:CDS 50.0%
AGTGAGGGACATTCAAGGTG+AGG + chr2.4:32653396-32653415 MS.gene77102:CDS 50.0%
CGGACATACGAAGTTCGAGA+AGG + chr2.4:32653754-32653773 MS.gene77102:CDS 50.0%
CTCGAACTTCGTATGTCCGT+AGG - chr2.4:32653753-32653772 None:intergenic 50.0%
GAAGCCAGTAACGGTATCTG+AGG + chr2.4:32651633-32651652 MS.gene77102:CDS 50.0%
GATCCCTCAGATACCGTTAC+TGG - chr2.4:32651640-32651659 None:intergenic 50.0%
GATTTCCATGAGCATAGGGC+TGG + chr2.4:32652601-32652620 MS.gene77102:intron 50.0%
GCCAAGCCAGCAAATTCTTC+AGG - chr2.4:32653427-32653446 None:intergenic 50.0%
GCTATCCCAGTTTCGCTATC+AGG + chr2.4:32652788-32652807 MS.gene77102:intron 50.0%
GCTGAAGGAATAGCTAGCCA+AGG - chr2.4:32653478-32653497 None:intergenic 50.0%
GGAATAGCTAGCCAAGGTTG+AGG - chr2.4:32653472-32653491 None:intergenic 50.0%
GTGAGGGACATTCAAGGTGA+GGG + chr2.4:32653397-32653416 MS.gene77102:CDS 50.0%
GTTCTCTTCCTTGCAAGAGG+AGG + chr2.4:32653821-32653840 MS.gene77102:CDS 50.0%
TCCTTGAAATTCCGGGTTCG+CGG - chr2.4:32652314-32652333 None:intergenic 50.0%
TGAGGGACATTCAAGGTGAG+GGG + chr2.4:32653398-32653417 MS.gene77102:CDS 50.0%
TTATGAAGGGATGCCTGCCA+TGG - chr2.4:32652501-32652520 None:intergenic 50.0%
TTGCAAGAGGAGGCAACGTT+CGG + chr2.4:32653831-32653850 MS.gene77102:CDS 50.0%
TTGGCATGCTGGAGTATGGT+CGG - chr2.4:32652423-32652442 None:intergenic 50.0%
! ATAGCGCCGCGGTTTTCATA+GGG - chr2.4:32652773-32652792 None:intergenic 50.0%
! TGACCTCTAGCTTCACCACA+TGG - chr2.4:32653907-32653926 None:intergenic 50.0%
!! AACGGTGATGACGGCTCTAA+TGG + chr2.4:32653799-32653818 MS.gene77102:CDS 50.0%
!! ACGGGAAGTACCAGTGAAGT+TGG - chr2.4:32653587-32653606 None:intergenic 50.0%
AAATTCCGGGTTCGCGGGTT+GGG - chr2.4:32652308-32652327 None:intergenic 55.0%
AAGAGGAGGCAACGTTCGGA+TGG + chr2.4:32653835-32653854 MS.gene77102:CDS 55.0%
ACCGTGGCCACCATTTGTGA+AGG + chr2.4:32652562-32652581 MS.gene77102:intron 55.0%
ACGTTCGGATGGAGCTAGTG+TGG + chr2.4:32653846-32653865 MS.gene77102:CDS 55.0%
ATCTGCATGTGTGCCCAACG+TGG + chr2.4:32652901-32652920 MS.gene77102:intron 55.0%
CCATACTCCAGCATGCCAAG+GGG + chr2.4:32652424-32652443 MS.gene77102:intron 55.0%
CCCTTCACAAATGGTGGCCA+CGG - chr2.4:32652566-32652585 None:intergenic 55.0%
CCGTGGCCACCATTTGTGAA+GGG + chr2.4:32652563-32652582 MS.gene77102:intron 55.0%
CCTTGAAATTCCGGGTTCGC+GGG - chr2.4:32652313-32652332 None:intergenic 55.0%
CTCATCCAGCCCTATGCTCA+TGG - chr2.4:32652609-32652628 None:intergenic 55.0%
CTTAGTCACTGCGGGAGTGA+GGG + chr2.4:32653381-32653400 MS.gene77102:CDS 55.0%
GAACGTTGCCTCCTCTTGCA+AGG - chr2.4:32653832-32653851 None:intergenic 55.0%
GACCATACTCCAGCATGCCA+AGG + chr2.4:32652422-32652441 MS.gene77102:intron 55.0%
GGTGAAGCTAGAGGTCACAC+TGG + chr2.4:32653910-32653929 MS.gene77102:CDS 55.0%
TCTGCCTCACAATTCCACCG+TGG + chr2.4:32652546-32652565 MS.gene77102:intron 55.0%
TTCACAAATGGTGGCCACGG+TGG - chr2.4:32652563-32652582 None:intergenic 55.0%
TTGACTTCCTTGGCCATGGC+AGG + chr2.4:32652485-32652504 MS.gene77102:intron 55.0%
TTTGCTACCCCTTGGCATGC+TGG - chr2.4:32652434-32652453 None:intergenic 55.0%
! GATAGCGCCGCGGTTTTCAT+AGG - chr2.4:32652774-32652793 None:intergenic 55.0%
! TAGCGCCGCGGTTTTCATAG+GGG - chr2.4:32652772-32652791 None:intergenic 55.0%
CCCCTTGGCATGCTGGAGTA+TGG - chr2.4:32652427-32652446 None:intergenic 60.0%
CCCGCGAACCCGGAATTTCA+AGG + chr2.4:32652310-32652329 MS.gene77102:intron 60.0%
GAAATTCCGGGTTCGCGGGT+TGG - chr2.4:32652309-32652328 None:intergenic 60.0%
GCAAGGGCAGTTGCTCCTAC+AGG + chr2.4:32651919-32651938 MS.gene77102:CDS 60.0%
GCGGGAGTGAGGGACATTCA+AGG + chr2.4:32653391-32653410 MS.gene77102:CDS 60.0%
GCTTAGTCACTGCGGGAGTG+AGG + chr2.4:32653380-32653399 MS.gene77102:CDS 60.0%
GTGGCCACGGTGGAATTGTG+AGG - chr2.4:32652553-32652572 None:intergenic 60.0%
AGGGATGCCTGCCATGGCCA+AGG - chr2.4:32652495-32652514 None:intergenic 65.0%
GCGAAACTGGGATAGCGCCG+CGG - chr2.4:32652784-32652803 None:intergenic 65.0%
CTGGACCCAACCCGCGAACC+CGG + chr2.4:32652300-32652319 MS.gene77102:intron 70.0%
!! CGGGTTCGCGGGTTGGGTCC+AGG - chr2.4:32652302-32652321 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 32651568 32653960 32651568 ID=MS.gene77102
chr2.4 mRNA 32651568 32653960 32651568 ID=MS.gene77102.t1;Parent=MS.gene77102
chr2.4 exon 32651568 32651989 32651568 ID=MS.gene77102.t1.exon1;Parent=MS.gene77102.t1
chr2.4 CDS 32651568 32651989 32651568 ID=cds.MS.gene77102.t1;Parent=MS.gene77102.t1
chr2.4 exon 32653351 32653595 32653351 ID=MS.gene77102.t1.exon2;Parent=MS.gene77102.t1
chr2.4 CDS 32653351 32653595 32653351 ID=cds.MS.gene77102.t1;Parent=MS.gene77102.t1
chr2.4 exon 32653728 32653960 32653728 ID=MS.gene77102.t1.exon3;Parent=MS.gene77102.t1
chr2.4 CDS 32653728 32653960 32653728 ID=cds.MS.gene77102.t1;Parent=MS.gene77102.t1
Gene Sequence

>MS.gene77102

ATGTCATTTAATAGAACAATTAGTAACGGTGATTTGGCAATTGTTTATGCAAGGCATGACAACATGAAGCCAGTAACGGTATCTGAGGGATCGGTTTTACAGAATCGATTCGGTGTTTTCAAACATTCAGATTGGATAGGAAAGTCATTTGGGTCTAAAGTATTCAGCAATAAGGGCGGTTTTGTTTACTTATTAGCACCTACTCCTGAATTGTGGACTTTAGTTTTAAGCCACAGGACACAGATTCTATATGTTGCAGATATCAGCTTTGTTGTTATGTATTTGGAGATTGTTCCTGGTTGTGTCGTTCTTGAATCTGGTACTGGAAGTGGGTCTTTAACTACTTCACTTGCAAGGGCAGTTGCTCCTACAGGACATGTTTATACGTTTGATTTTCACGAACAAAGATCTGGATCAGCTAGGTAATACTGCACTTTTCTTTTTCTGACGATGTTTGATATACATGTGCTTATCTTTAGGCAATGTTGTATGTGTTATTTTCTAGTGTTGTCAAATATTGGCGCTATAGCATAGCGGATTTTGATGAATCCGCTAAAAAAACTGCAACACTATCATAGTTTTGCCGTTATTCTAGTTTTTTGGTAGTTGATGTAGCAACAATAGCAGAAAAAAGAGACTTTTAGGCTGTGTTGTCAATTGTGGAAGGCTAAAAATCTGTCATATAAACACGCCATTACCGCCTAAAAAACGTATTTTTGGTGAAACTGGGTCCTGGACCCAACCCGCGAACCCGGAATTTCAAGGAAGATAACCTGAATCAATGAATTGCAAGATTCCGCCGTACCTACAATTTTCTGCGGTGATGCTGGCCGCACCCACCAAATTCTGATTCCGACCATACTCCAGCATGCCAAGGGGTAGCAAAATCATACGATCTCATGAGTTTGATCGTGATTTTGACTTCCTTGGCCATGGCAGGCATCCCTTCATAAATTGGCTATGGCGGGCGTAAAAAAATCTGCCTCACAATTCCACCGTGGCCACCATTTGTGAAGGGCATGTTTATACATTTGATTTCCATGAGCATAGGGCTGGATGAGCTATGCAATACACCAGACTTCCTCTGATGTTTGATACATGTGTGTTTATCTTTAGCAAATGTCGAGTATGTTTTTTTCAGTGTTGTCAAATTGATTCAATAGCGGCACTATTGTGCTATCACATGGTGGATTTTGATGATCCCCTATGAAAACCGCGGCGCTATCCCAGTTTCGCTATCAGGGCAATAGCGGCAGCTATTAAAGTTTTGCTAGCTGATATACGAGCAATAGTGTAATTTTTTTTCATTTCTTTTAAATAATAAAATTGAAGAATCTGCATGTGTGCCCAACGTGGCTTATTTTTACAATGGAAAAACTTAGGAGTCGGTGAGTATTACTCCTGTATTCACCGTAATACAATATATAAGCTATGCTCTGCCTCCATTCCCTTTTTTATTTGTATGTAGGGTGCCTTGTTTTATATATAATTGACTTATTTCAACTGTTATGCGGTTTCTGCTATTTGCCCGTGTTACTATCTTCTATTTCACATAGCGGTTTTCCGTAATATGCTATTGATGGCACTTTCATGGCTTTGCATTATATTGTGTTCTCTTTTTTCCAGTAATTGCAAGTAATATTATTGGTGATCTAATACGCTACTGCTCAGAATGTCAACGCTCAAGTTCAAATTTATTTAACTGTACTCAATGCGGTGATAAACTTGAGATGTTGAGTGTAAAGGTAAATTACAGATATGAATATTGTTTTTCTTCTTGTAGGGATGACTTTGAGAAGATTGGCATAAGCAGCTTAGTCACTGCGGGAGTGAGGGACATTCAAGGTGAGGGGTTTCCTGAAGAATTTGCTGGCTTGGCTGATGCAGTTTTTTTGGATCTACCTCAACCTTGGCTAGCTATTCCTTCAGCTGCAAAAATGTTACGACATGACGGCACTTTATGTTCTTTCTCCCCGTGTATTGAACAAGTACAACGATCATGTGAAACAATGCAATCCAACTTCACTGGTACTTCCCGTTTTTTCTTCTTTTATATGTTTCTTATTTTCGCGCTCTCTCACACGTAGTAGTTATGCAACTAATGGTAATTTTATTTAAGCACTAACAAAACTCCTTTGTATACGTTTGTTTATGTGAAAGATATAAGGACTTTTGAGGTACTCCTACGGACATACGAAGTTCGAGAAGGGAAAATGGAAAGCTTAGAGATTAACGGTGATGACGGCTCTAATGGTTCTCTTCCTTGCAAGAGGAGGCAACGTTCGGATGGAGCTAGTGTGGTTACCTATCCTAATTCTTCTTCTGTTATGGCTAGACCATGTGGTGAAGCTAGAGGTCACACTGGTTATTTGACATTTGCAAGACTTAATTAA
Protein sequence

>MS.gene77102.t1

MSFNRTISNGDLAIVYARHDNMKPVTVSEGSVLQNRFGVFKHSDWIGKSFGSKVFSNKGGFVYLLAPTPELWTLVLSHRTQILYVADISFVVMYLEIVPGCVVLESGTGSGSLTTSLARAVAPTGHVYTFDFHEQRSGSARDDFEKIGISSLVTAGVRDIQGEGFPEEFAGLADAVFLDLPQPWLAIPSAAKMLRHDGTLCSFSPCIEQVQRSCETMQSNFTDIRTFEVLLRTYEVREGKMESLEINGDDGSNGSLPCKRRQRSDGASVVTYPNSSSVMARPCGEARGHTGYLTFARLN