AlfalfaGEDB | MS.gene77682

M. sativa cultivar XinJiangDaYe / MS.gene77682

KEGG: K13095
GO: GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF00013.32 PF00076.25 PF16275.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77682.t1	XP_013455377.1	91.8	767	59	1	1	763	1	767	8.50E-282	979.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77682.t1	Q9LU44	83.8	433	62	3	105	530	132	563	9.8e-186	651.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77682.t1	A0A072UK27	91.8	767	59	1	1	763	1	767	6.2e-282	979.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049297	MS.gene77682	-0.808701	2.72E-50	-1.69E-46
MS.gene050227	MS.gene77682	-0.808369	3.20E-50	-1.69E-46
MS.gene050228	MS.gene77682	-0.802144	6.45E-49	-1.69E-46
MS.gene050303	MS.gene77682	-0.816096	6.59E-52	-1.69E-46
MS.gene050330	MS.gene77682	0.832684	8.22E-56	-1.69E-46
MS.gene050623	MS.gene77682	0.805335	1.40E-49	-1.69E-46
MS.gene052176	MS.gene77682	-0.858016	1.10E-62	-1.69E-46
MS.gene052302	MS.gene77682	-0.809825	1.56E-50	-1.69E-46
MS.gene052309	MS.gene77682	0.879368	1.32E-69	-1.69E-46
MS.gene052358	MS.gene77682	0.850385	1.76E-60	-1.69E-46
MS.gene052361	MS.gene77682	0.800018	1.75E-48	-1.69E-46
MS.gene052376	MS.gene77682	-0.823615	1.26E-53	-1.69E-46
MS.gene053054	MS.gene77682	0.81689	4.38E-52	-1.69E-46
MS.gene054291	MS.gene77682	0.801118	1.05E-48	-1.69E-46
MS.gene054473	MS.gene77682	-0.804893	1.74E-49	-1.69E-46
MS.gene054930	MS.gene77682	-0.800821	1.20E-48	-1.69E-46
MS.gene054942	MS.gene77682	-0.800071	1.71E-48	-1.69E-46
MS.gene054983	MS.gene77682	-0.81219	4.80E-51	-1.69E-46
MS.gene056069	MS.gene77682	0.834538	2.83E-56	-1.69E-46
MS.gene056087	MS.gene77682	-0.825963	3.52E-54	-1.69E-46
MS.gene058200	MS.gene77682	-0.817604	3.03E-52	-1.69E-46
MS.gene05954	MS.gene77682	-0.821978	3.03E-53	-1.69E-46
MS.gene060137	MS.gene77682	0.815583	8.58E-52	-1.69E-46
MS.gene061040	MS.gene77682	0.903046	5.20E-79	-1.69E-46
MS.gene061114	MS.gene77682	0.828619	8.13E-55	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene77682	MS.gene069405	PPI
MS.gene77682	MS.gene015344	PPI
MS.gene037361	MS.gene77682	PPI
MS.gene067412	MS.gene77682	PPI
MS.gene93132	MS.gene77682	PPI
MS.gene77682	MS.gene86409	PPI
MS.gene09686	MS.gene77682	PPI
MS.gene21500	MS.gene77682	PPI
MS.gene77682	MS.gene63990	PPI
MS.gene77682	MS.gene83541	PPI
MS.gene64193	MS.gene77682	PPI
MS.gene54212	MS.gene77682	PPI
MS.gene77682	MS.gene24187	PPI
MS.gene77682	MS.gene029212	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77682.t1	MTR_4g036415	98.305	767	9	1	1	763	1	767	0.0	1514
MS.gene77682.t1	MTR_2g005590	47.154	123	59	2	213	329	162	284	1.98e-26	110
MS.gene77682.t1	MTR_7g074100	46.341	123	60	3	213	329	136	258	6.65e-26	108
MS.gene77682.t1	MTR_8g005550	44.776	134	67	4	202	329	121	253	1.08e-25	107
MS.gene77682.t1	MTR_8g006580	44.776	134	67	4	202	329	121	253	1.08e-25	107
MS.gene77682.t1	MTR_8g006605	44.776	134	67	4	202	329	121	253	1.08e-25	107
MS.gene77682.t1	MTR_8g005430	44.361	133	68	3	202	329	121	252	1.87e-25	107
MS.gene77682.t1	MTR_2g005590	46.774	124	59	3	213	329	162	285	7.62e-25	106
MS.gene77682.t1	MTR_3g010220	39.623	159	85	5	180	329	113	269	1.92e-24	104
MS.gene77682.t1	MTR_3g083770	30.973	226	119	6	91	304	68	268	1.92e-24	109
MS.gene77682.t1	MTR_3g083770	30.973	226	118	6	91	304	68	267	2.65e-24	109
MS.gene77682.t1	MTR_5g075710	44.800	125	59	4	213	329	135	257	3.32e-24	103
MS.gene77682.t1	MTR_5g075710	44.800	125	59	4	213	329	120	242	5.11e-24	102
MS.gene77682.t1	MTR_5g075710	43.902	123	63	2	213	329	98	220	3.02e-22	97.1
MS.gene77682.t1	MTR_3g075500	45.000	80	44	0	449	528	208	287	2.29e-14	74.7
MS.gene77682.t1	MTR_4g070080	45.946	74	40	0	450	523	9	82	4.10e-14	70.1
MS.gene77682.t1	MTR_4g081520	43.820	89	46	2	446	534	50	134	5.79e-14	70.1
MS.gene77682.t1	MTR_4g070080	45.946	74	40	0	450	523	9	82	9.22e-14	70.1
MS.gene77682.t1	MTR_3g062640	40.000	100	50	3	213	304	136	233	9.99e-14	72.0
MS.gene77682.t1	MTR_7g013740	36.782	87	55	0	442	528	35	121	2.12e-12	65.9
MS.gene77682.t1	MTR_4g070190	41.892	74	43	0	450	523	11	84	2.94e-12	65.9
MS.gene77682.t1	MTR_7g013730	34.091	88	58	0	442	529	35	122	3.91e-12	67.4
MS.gene77682.t1	MTR_4g104470	40.506	79	47	0	450	528	219	297	5.89e-12	67.8
MS.gene77682.t1	MTR_4g109540	35.366	82	53	0	447	528	42	123	6.88e-12	67.0
MS.gene77682.t1	MTR_1g045510	42.667	75	43	0	449	523	206	280	2.76e-11	65.5
MS.gene77682.t1	MTR_4g070140	43.243	74	42	0	450	523	11	84	3.58e-11	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77682.t1	AT5G51300	66.957	805	196	18	5	763	24	804	0.0	944
MS.gene77682.t1	AT5G51300	66.957	805	196	18	5	763	24	804	0.0	944
MS.gene77682.t1	AT5G51300	66.957	805	196	18	5	763	24	804	0.0	944
MS.gene77682.t1	AT1G09660	46.565	131	62	3	207	329	142	272	5.06e-28	115
MS.gene77682.t1	AT1G09660	46.565	131	62	3	207	329	142	272	5.06e-28	115
MS.gene77682.t1	AT3G08620	44.361	133	67	3	202	328	83	214	6.19e-26	107
MS.gene77682.t1	AT3G08620	44.361	133	67	3	202	328	128	259	7.91e-26	108
MS.gene77682.t1	AT2G38610	43.704	135	69	3	202	330	129	262	3.40e-25	106
MS.gene77682.t1	AT2G38610	43.704	135	69	3	202	330	129	262	3.40e-25	106
MS.gene77682.t1	AT4G26480	44.715	123	62	3	213	329	78	200	5.73e-24	101
MS.gene77682.t1	AT4G26480	44.715	123	62	3	213	329	163	285	1.24e-23	102
MS.gene77682.t1	AT4G26480	44.715	123	62	3	213	329	163	285	1.24e-23	102
MS.gene77682.t1	AT5G56140	43.902	123	63	3	213	329	169	291	2.18e-23	102
MS.gene77682.t1	AT3G32940	29.767	215	102	7	97	304	55	227	1.40e-18	90.9
MS.gene77682.t1	AT1G09660	42.105	114	56	4	207	312	142	253	3.07e-16	80.1
MS.gene77682.t1	AT4G24770	45.570	79	43	0	449	527	246	324	8.16e-15	77.0
MS.gene77682.t1	AT4G24770	45.570	79	43	0	449	527	246	324	8.16e-15	77.0
MS.gene77682.t1	AT4G13860	41.026	78	46	0	447	524	3	80	3.42e-14	68.9
MS.gene77682.t1	AT3G23830	45.455	77	42	0	447	523	35	111	5.17e-13	67.4
MS.gene77682.t1	AT3G23830	45.455	77	42	0	447	523	35	111	5.17e-13	67.4
MS.gene77682.t1	AT5G54580	41.026	78	46	0	446	523	55	132	6.01e-13	67.8
MS.gene77682.t1	AT1G18630	44.156	77	43	0	447	523	36	112	6.41e-13	67.8
MS.gene77682.t1	AT4G13850	40.000	105	60	2	423	527	14	115	7.11e-13	66.6
MS.gene77682.t1	AT5G50250	44.000	75	42	0	449	523	209	283	9.87e-13	70.1
MS.gene77682.t1	AT4G39260	40.541	74	44	0	450	523	9	82	1.97e-12	64.3
MS.gene77682.t1	AT5G47320	43.210	81	46	0	447	527	31	111	3.08e-12	67.0
MS.gene77682.t1	AT4G13850	40.000	105	60	2	423	527	14	115	3.71e-12	65.1
MS.gene77682.t1	AT4G13850	40.000	105	60	2	423	527	14	115	4.58e-12	65.1
MS.gene77682.t1	AT4G39260	40.541	74	44	0	450	523	9	82	4.81e-12	64.3
MS.gene77682.t1	AT4G13850	40.000	105	60	2	423	527	14	115	5.13e-12	65.1
MS.gene77682.t1	AT1G03457	42.667	75	43	0	448	522	226	300	5.69e-12	68.2
MS.gene77682.t1	AT1G03457	42.667	75	43	0	448	522	340	414	1.04e-11	68.2
MS.gene77682.t1	AT5G61030	37.500	80	50	0	444	523	37	116	1.05e-11	67.0
MS.gene77682.t1	AT5G61030	37.500	80	50	0	444	523	37	116	1.05e-11	67.0
MS.gene77682.t1	AT1G03457	42.667	75	43	0	448	522	331	405	1.16e-11	68.2
MS.gene77682.t1	AT4G39260	40.541	74	44	0	450	523	9	82	1.30e-11	64.3
MS.gene77682.t1	AT2G21660	40.541	74	44	0	450	523	11	84	1.55e-11	63.5
MS.gene77682.t1	AT2G21660	40.541	74	44	0	450	523	11	84	2.23e-11	63.5
MS.gene77682.t1	AT2G37220	40.244	82	48	1	446	527	90	170	3.22e-11	65.5
MS.gene77682.t1	AT5G06210	36.634	101	56	2	447	540	34	133	6.26e-11	61.6

Find 292 sgRNAs with CRISPR-Local

Find 337 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTATTCAATGTTTGAATTTC+AGG	0.055676	4.4:+67505527	None:intergenic
ATCCAATTGTTTCAACAATT+TGG	0.151016	4.4:-67504510	MS.gene77682:CDS
CCTCCTTGGGCTAACAATTC+TGG	0.215980	4.4:-67504648	MS.gene77682:CDS
TGAACCTATATATGATAATA+TGG	0.217428	4.4:-67505423	MS.gene77682:CDS
ATCTTTGTCGGTTCCGCTTC+GGG	0.224492	4.4:+67505739	None:intergenic
TGTTATGGCAAAAGTTATTA+AGG	0.246290	4.4:-67504481	MS.gene77682:CDS
CCAACATATCCTGCATCTTA+TGG	0.257559	4.4:-67503886	MS.gene77682:CDS
CCGTTCTGATCGTTTGAATC+TGG	0.262072	4.4:+67505653	None:intergenic
CGGCTTTGAATGGGACGATT+AGG	0.278069	4.4:-67504941	MS.gene77682:CDS
GCAATTGGTTCGGGCAGTTC+TGG	0.291106	4.4:-67504678	MS.gene77682:CDS
TGGTGCCGTTGATGCCGTTT+TGG	0.296938	4.4:+67505840	None:intergenic
AATTCAACTTCCTGATTTCA+TGG	0.302742	4.4:-67505567	MS.gene77682:CDS
GAATGAGGATTTGCATGTTT+TGG	0.305991	4.4:-67505078	MS.gene77682:CDS
TGGGCTAACAATTCTGGTTC+TGG	0.308759	4.4:-67504642	MS.gene77682:CDS
TGTAGGTTGTGTGGTGAGCC+TGG	0.310565	4.4:-67504906	MS.gene77682:CDS
CAGAATTTCTTGGCTGAGTT+GGG	0.312746	4.4:-67504744	MS.gene77682:CDS
GTTGTCTTAATCGCAGATTC+AGG	0.313080	4.4:+67504711	None:intergenic
CGGTTCGTCATCAGCCCATC+TGG	0.320048	4.4:+67505598	None:intergenic
GATCTTTGTCGGTTCCGCTT+CGG	0.321344	4.4:+67505738	None:intergenic
TGGTGCTAAGATTGTGATTC+GGG	0.329516	4.4:-67505165	MS.gene77682:CDS
TCCGCCTGATTGATCTTTGT+CGG	0.336120	4.4:+67505727	None:intergenic
GGATTGTATGGAGGAGGAAT+AGG	0.337627	4.4:+67504156	None:intergenic
AAATCAGGAAGTTGAATTAC+CGG	0.338043	4.4:+67505572	None:intergenic
CACCAAATTGTTGAAACAAT+TGG	0.341079	4.4:+67504508	None:intergenic
AGTACACCACAAGCTGGATT+AGG	0.345327	4.4:-67504615	MS.gene77682:CDS
AGGGGCTGGTGCGGTTAAAA+AGG	0.349731	4.4:-67504595	MS.gene77682:CDS
TAGTACTTTGGCAATTGGTT+CGG	0.354026	4.4:-67504688	MS.gene77682:CDS
GGCTGATGACGAACCGAAAC+CGG	0.354826	4.4:-67505591	MS.gene77682:CDS
GTACACCACAAGCTGGATTA+GGG	0.357290	4.4:-67504614	MS.gene77682:CDS
GGAAGTTGAATTACCGGTTT+CGG	0.361177	4.4:+67505578	None:intergenic
GGCTTTGAATGGGACGATTA+GGG	0.361903	4.4:-67504940	MS.gene77682:CDS
CCCATTGGGAGTGCTCCTCC+TGG	0.362255	4.4:-67504213	MS.gene77682:CDS
TAAAGCTTCTTCTGAAGCTT+TGG	0.365077	4.4:+67505278	None:intergenic
ACAATTTGGTGAGATTGTTA+TGG	0.367806	4.4:-67504496	MS.gene77682:CDS
ATGATACAAATTTGTACATT+GGG	0.378528	4.4:-67504566	MS.gene77682:CDS
AGTGCTCCTCCTGGGAGTTA+TGG	0.379254	4.4:-67504204	MS.gene77682:CDS
AGTACTTTGGCAATTGGTTC+GGG	0.379590	4.4:-67504687	MS.gene77682:CDS
GATTAAGACAACTAGTACTT+TGG	0.382369	4.4:-67504700	MS.gene77682:CDS
GGAGCTGCTGGTGGCATTGA+AGG	0.382420	4.4:+67503910	None:intergenic
GTTCTGGAAGCAATCCTCCT+TGG	0.384094	4.4:-67504662	MS.gene77682:CDS
CAGCATTGCAAATGCGCCTT+GGG	0.384172	4.4:-67503788	MS.gene77682:CDS
AGGAGGGGCGGAATGATTAC+TGG	0.384270	4.4:+67503765	None:intergenic
GATGCGCTTGAGGCTGCGGC+TGG	0.389783	4.4:-67505038	MS.gene77682:CDS
TATATGATAATATGGGGATT+AGG	0.390370	4.4:-67505415	MS.gene77682:CDS
TGGACGTGAATCGGTGTTAT+CGG	0.392557	4.4:+67505811	None:intergenic
AGGGAGCTTGCGGCTTTGAA+TGG	0.394860	4.4:-67504951	MS.gene77682:CDS
GTTTGTAAGCATTGTGGGGA+TGG	0.395994	4.4:-67504831	MS.gene77682:CDS
GGTTTATCGTTGAGCGGTAG+TGG	0.399756	4.4:+67505878	None:intergenic
ACAGCCGACGCAGGTGGGCC+TGG	0.401744	4.4:+67504303	None:intergenic
TCCTCATTCTCCGACGGATC+CGG	0.402901	4.4:+67505092	None:intergenic
TTGGGCTTATTATTGGGCCT+AGG	0.403109	4.4:-67505220	MS.gene77682:CDS
AGCATAAGAGGCAGCAACTT+AGG	0.403660	4.4:-67504971	MS.gene77682:CDS
CTCTCCGAAAACGGTTTAAC+CGG	0.404174	4.4:-67505770	MS.gene77682:CDS
ACAACTAGTACTTTGGCAAT+TGG	0.404593	4.4:-67504693	MS.gene77682:CDS
CCAATTGGCTGGCTTTGAAC+AGG	0.407319	4.4:+67504270	None:intergenic
CCACCCACTCTTGAGGATGA+TGG	0.408818	4.4:-67504537	MS.gene77682:CDS
TGGTCTGAAGTAGGGGCAGG+AGG	0.410645	4.4:+67503817	None:intergenic
CCGCCACCGGGACGAAGAAA+AGG	0.411481	4.4:-67505625	MS.gene77682:CDS
TTCTGGAAGCAATCCTCCTT+GGG	0.414696	4.4:-67504661	MS.gene77682:CDS
GAACCTATATATGATAATAT+GGG	0.415791	4.4:-67505422	MS.gene77682:CDS
TGTCCGGTGAAGGGTGCTAC+TGG	0.415903	4.4:-67504789	MS.gene77682:CDS
GAGGCACATTTCCAATGCTC+TGG	0.415977	4.4:+67503719	None:intergenic
TCCGCTTCGGGTGTTGCCGC+CGG	0.416865	4.4:+67505751	None:intergenic
TCAATACCACCCATGAAATC+AGG	0.417840	4.4:+67505557	None:intergenic
ACTATTGCAGTAAGAGTTGC+TGG	0.418096	4.4:-67504351	MS.gene77682:CDS
GTCTGAAGTAGGGGCAGGAG+GGG	0.418816	4.4:+67503819	None:intergenic
GGTCAACCTATGCCTCCTTA+TGG	0.419163	4.4:-67504087	MS.gene77682:CDS
CCTCAGCCTGTTGTACCTCC+AGG	0.422104	4.4:-67504321	MS.gene77682:CDS
TGGAATCGGTGGACGTGAAT+CGG	0.422421	4.4:+67505802	None:intergenic
GAAGGAGATCTAGCCCCTTC+AGG	0.424049	4.4:+67505449	None:intergenic
CATTTGCAATGCTGTGTGAT+TGG	0.424259	4.4:+67503797	None:intergenic
TATACCGGTGGCGGTCCCAT+TGG	0.425842	4.4:-67504228	MS.gene77682:CDS
TGCTGCATTTCACCAGAAGC+AGG	0.430915	4.4:+67504012	None:intergenic
TATACCGATGAAAGAGTATC+CGG	0.432063	4.4:-67505255	MS.gene77682:CDS
TCGGCGGAAAAGACGTCATA+TGG	0.435235	4.4:-67503673	MS.gene77682:CDS
TGATCGTTTGAATCTGGCTG+TGG	0.435288	4.4:+67505659	None:intergenic
TGGGCTTATTATTGGGCCTA+GGG	0.437365	4.4:-67505219	MS.gene77682:CDS
TCTGTACCGACCAACATGTA+TGG	0.438751	4.4:-67503943	MS.gene77682:CDS
ATACCGGTGGCGGTCCCATT+GGG	0.441379	4.4:-67504227	MS.gene77682:CDS
CTGGTGCTAAGATTGTGATT+CGG	0.442727	4.4:-67505166	MS.gene77682:CDS
AAGATTGTGATTCGGGGTAA+AGG	0.445533	4.4:-67505158	MS.gene77682:CDS
AGCTTCTTCTGAAGCTTTGG+TGG	0.445877	4.4:+67505281	None:intergenic
TTAGGGATGAAGAGTATTGT+AGG	0.446170	4.4:-67504923	MS.gene77682:CDS
GTTATTAAGGATCGAATGAC+AGG	0.447306	4.4:-67504468	MS.gene77682:CDS
ACCGGATCCGTCGGAGAATG+AGG	0.448730	4.4:-67505093	MS.gene77682:CDS
TTAAACGTCGAAGTCCTTGT+AGG	0.449328	4.4:+67504864	None:intergenic
ACAGCATTGCAAATGCGCCT+TGG	0.451912	4.4:-67503789	MS.gene77682:CDS
CACGCATACTGCCGATGCCC+AGG	0.452453	4.4:+67504888	None:intergenic
GCTATTGCAGCCTGTGGATG+AGG	0.452749	4.4:-67505003	MS.gene77682:CDS
GGGAGCTTGCGGCTTTGAAT+GGG	0.453782	4.4:-67504950	MS.gene77682:CDS
GGTGGGCCTGGAGGTACAAC+AGG	0.456453	4.4:+67504315	None:intergenic
AATCCACTTCTCTCCGAAAA+CGG	0.458472	4.4:-67505779	MS.gene77682:CDS
TGATGAGTATCAGAATTTCT+TGG	0.459979	4.4:-67504754	MS.gene77682:CDS
ATAAGTAGAATGTTGCAATC+TGG	0.461645	4.4:-67505491	MS.gene77682:CDS
CCATTGGGAGTGCTCCTCCT+GGG	0.463144	4.4:-67504212	MS.gene77682:CDS
ACCGCCACCGGTATATTGTT+GGG	0.463821	4.4:+67504236	None:intergenic
GGCTGATGCGCTTGAGGCTG+CGG	0.465364	4.4:-67505042	MS.gene77682:CDS
TGGAGGCACTCCTGTCCCAT+GGG	0.466274	4.4:-67504184	MS.gene77682:CDS
AGGTTGTTTGTAAGCATTGT+GGG	0.470243	4.4:-67504836	MS.gene77682:CDS
ATCGTTGAGCGGTAGTGGAT+TGG	0.472413	4.4:+67505883	None:intergenic
CTCAAGGCGATAGCCATTCA+TGG	0.472803	4.4:+67504380	None:intergenic
GCTTGAGGCTGCGGCTGGTA+TGG	0.473691	4.4:-67505033	MS.gene77682:CDS
GACCGCCACCGGTATATTGT+TGG	0.475580	4.4:+67504235	None:intergenic
TTCTGAGTATGAGAAATTCA+TGG	0.476992	4.4:-67503644	MS.gene77682:CDS
GCATAAGGATTGTATGGAGG+AGG	0.478828	4.4:+67504150	None:intergenic
GGATATGTTGGTGGAGCTGC+TGG	0.481070	4.4:+67503898	None:intergenic
GATGATACAAATTTGTACAT+TGG	0.481380	4.4:-67504567	MS.gene77682:CDS
TGCAGCAGAATTACCCTCCT+GGG	0.481585	4.4:-67503996	MS.gene77682:CDS
GCATAAGAGGCAGCAACTTA+GGG	0.481868	4.4:-67504970	MS.gene77682:CDS
ATGAATGGCTATCGCCTTGA+GGG	0.484462	4.4:-67504378	MS.gene77682:CDS
GATACATAGTTGATCCAGGC+GGG	0.485027	4.4:+67504121	None:intergenic
TCAGAATTTCTTGGCTGAGT+TGG	0.489105	4.4:-67504745	MS.gene77682:CDS
AGATTGTGATTCGGGGTAAA+GGG	0.490400	4.4:-67505157	MS.gene77682:CDS
AAGAGGATGGAGAAGGAGAC+TGG	0.491145	4.4:-67505185	MS.gene77682:CDS
TCTGAAGTAGGGGCAGGAGG+GGG	0.491665	4.4:+67503820	None:intergenic
ATGGATAGGCACCAATTGGC+TGG	0.493844	4.4:+67504259	None:intergenic
TTATTATCAGACTGCACCCC+AGG	0.496388	4.4:+67503979	None:intergenic
ATCCCCATATTATCATATAT+AGG	0.497159	4.4:+67505419	None:intergenic
GAGAGAAGTGGATTTGGAAT+CGG	0.498073	4.4:+67505788	None:intergenic
AATGCAATTGTTGCCATGAA+TGG	0.498742	4.4:-67504393	MS.gene77682:CDS
GTGGGCACAGCCGACGCAGG+TGG	0.500778	4.4:+67504297	None:intergenic
CCTTTAGATGATCGTCCTGA+AGG	0.501343	4.4:-67505464	MS.gene77682:CDS
GGGGACCTGTCGGCATGGGT+GGG	0.503889	4.4:+67504055	None:intergenic
TCATCCTAGTATTGATTGTC+CGG	0.504611	4.4:-67504805	MS.gene77682:CDS
CAGGTTTGAGTAAAGGATAC+GGG	0.505016	4.4:-67504449	MS.gene77682:CDS
CCTGTTCAAAGCCAGCCAAT+TGG	0.505090	4.4:-67504270	MS.gene77682:CDS
CTGGCTTTGAACAGGATAAG+TGG	0.506527	4.4:+67504278	None:intergenic
GCAGAAGAGGGATTTGAAAC+CGG	0.508144	4.4:-67505111	MS.gene77682:CDS
TGCAGACGAAGGAGGAGGAG+GGG	0.508439	4.4:+67503750	None:intergenic
CCATAAGATGCAGGATATGT+TGG	0.508849	4.4:+67503886	None:intergenic
CTTTAGATGATCGTCCTGAA+GGG	0.510187	4.4:-67505463	MS.gene77682:CDS
GGAGGAGCATAAGGATTGTA+TGG	0.510415	4.4:+67504144	None:intergenic
TAGTATTGATTGTCCGGTGA+AGG	0.510881	4.4:-67504799	MS.gene77682:CDS
CTGTGTGATTGGTCTGAAGT+AGG	0.514366	4.4:+67503808	None:intergenic
CAGAACGGCGAAGCCGCCAC+CGG	0.515344	4.4:-67505638	MS.gene77682:CDS
GTGGGTCCCATCGGCTTCTA+CGG	0.516793	4.4:+67505678	None:intergenic
GGAGGAATAGGAGGTCCCCA+TGG	0.517280	4.4:+67504168	None:intergenic
CGAATGACAGGTTTGAGTAA+AGG	0.518211	4.4:-67504456	MS.gene77682:CDS
CCAGGAGGAGCACTCCCAAT+GGG	0.520762	4.4:+67504213	None:intergenic
CAACTTCCTGATTTCATGGG+TGG	0.520799	4.4:-67505563	MS.gene77682:CDS
TTCCCAGTAGCACCCTTCAC+CGG	0.521265	4.4:+67504786	None:intergenic
ATAGGAGGTCCCCATGGGAC+AGG	0.522360	4.4:+67504174	None:intergenic
ATTCAACTTCCTGATTTCAT+GGG	0.522392	4.4:-67505566	MS.gene77682:CDS
ACTCCCAATGGGACCGCCAC+CGG	0.525906	4.4:+67504224	None:intergenic
GTATTTGCCACCCACTCTTG+AGG	0.527496	4.4:-67504544	MS.gene77682:CDS
GGTGGGTATTGAACACCATA+AGG	0.528437	4.4:+67504072	None:intergenic
GGAGGAACTGCATTATAGTA+AGG	0.528530	4.4:+67503850	None:intergenic
TGGGATGGATAGGCACCAAT+TGG	0.528906	4.4:+67504255	None:intergenic
ATATTATCATATATAGGTTC+AGG	0.529213	4.4:+67505425	None:intergenic
ACAGGTTTGAGTAAAGGATA+CGG	0.530195	4.4:-67504450	MS.gene77682:CDS
ATGCAGCAGAATTACCCTCC+TGG	0.530640	4.4:-67503997	MS.gene77682:CDS
TCCGCCGATGAGTTAGGAGG+AGG	0.531623	4.4:+67503688	None:intergenic
GGTCTGAAGTAGGGGCAGGA+GGG	0.532121	4.4:+67503818	None:intergenic
GGTAAAGGGTCGGTGAAAGA+AGG	0.532993	4.4:-67505143	MS.gene77682:CDS
AAGGTAGGTTGCAGCAGAAG+AGG	0.533663	4.4:-67505124	MS.gene77682:CDS
TCTGCAGACGAAGGAGGAGG+AGG	0.534861	4.4:+67503748	None:intergenic
GGCAGTATGCGTGTCCTACA+AGG	0.536158	4.4:-67504878	MS.gene77682:CDS
GCAGATCAATCCCAGAGCAT+TGG	0.537143	4.4:-67503730	MS.gene77682:CDS
GAGTTAGGAGGAGGCATAGG+TGG	0.538669	4.4:+67503697	None:intergenic
GGAACCGACAAAGATCAATC+AGG	0.538815	4.4:-67505731	MS.gene77682:CDS
AGACGAAGGAGGAGGAGGGG+CGG	0.540123	4.4:+67503753	None:intergenic
AATTTCTTGGCTGAGTTGGG+TGG	0.540197	4.4:-67504741	MS.gene77682:CDS
ATGGAGGCACTCCTGTCCCA+TGG	0.540461	4.4:-67504185	MS.gene77682:CDS
AGGCACATTTCCAATGCTCT+GGG	0.542648	4.4:+67503720	None:intergenic
CTGCAGACGAAGGAGGAGGA+GGG	0.542706	4.4:+67503749	None:intergenic
GTAGGTTGTGTGGTGAGCCT+GGG	0.545604	4.4:-67504905	MS.gene77682:CDS
GTCCGGTGAAGGGTGCTACT+GGG	0.545922	4.4:-67504788	MS.gene77682:CDS
GGATTTGAAACCGGATCCGT+CGG	0.546961	4.4:-67505102	MS.gene77682:CDS
ATCGGTGTTATCGGTGGTGG+TGG	0.547897	4.4:+67505820	None:intergenic
GATCGTTTGAATCTGGCTGT+GGG	0.548883	4.4:+67505660	None:intergenic
GGAGGATACATAGTTGATCC+AGG	0.549406	4.4:+67504117	None:intergenic
GCTGCATTTCACCAGAAGCA+GGG	0.550120	4.4:+67504013	None:intergenic
TCAGCAGGTGGCTTAAAAGC+AGG	0.550868	4.4:+67505311	None:intergenic
GGAGGATATCCATAAGATGC+AGG	0.551040	4.4:+67503877	None:intergenic
GGTATATTGTTGGGATGGAT+AGG	0.552672	4.4:+67504245	None:intergenic
CAATACCCACCCATGCCGAC+AGG	0.553737	4.4:-67504060	MS.gene77682:CDS
CCTTCAGGACGATCATCTAA+AGG	0.555385	4.4:+67505464	None:intergenic
GCTCCTCCTGGGAGTTATGG+AGG	0.555783	4.4:-67504201	MS.gene77682:CDS
GCATTATAGTAAGGTGGAGG+AGG	0.556594	4.4:+67503859	None:intergenic
ATTGGAATCGAGAGCCTCCA+TGG	0.556663	4.4:+67505901	None:intergenic
TGAGGCTGCGGCTGGTATGG+TGG	0.557666	4.4:-67505030	MS.gene77682:CDS
AGGTAGGTTGCAGCAGAAGA+GGG	0.559912	4.4:-67505123	MS.gene77682:CDS
TTCACCGGACAATCAATACT+AGG	0.560422	4.4:+67504801	None:intergenic
TGAACACCATAAGGAGGCAT+AGG	0.560821	4.4:+67504081	None:intergenic
GGGTGCTACTGGGAAGAAGA+TGG	0.562289	4.4:-67504778	MS.gene77682:CDS
TCCCAACAATATACCGGTGG+CGG	0.562455	4.4:-67504237	MS.gene77682:CDS
CATGAATGGCTATCGCCTTG+AGG	0.563895	4.4:-67504379	MS.gene77682:CDS
TGTGTGGTGAGCCTGGGCAT+CGG	0.564193	4.4:-67504899	MS.gene77682:CDS
TTTGGTGGTCTATAATCAGC+AGG	0.566022	4.4:+67505296	None:intergenic
CTGCAATAGTTCGACCCTCA+AGG	0.566323	4.4:+67504364	None:intergenic
TGTGTGATTGGTCTGAAGTA+GGG	0.566402	4.4:+67503809	None:intergenic
ACCTCCAGGCCCACCTGCGT+CGG	0.566557	4.4:-67504307	MS.gene77682:CDS
AATCTGGCTGTGGGTCCCAT+CGG	0.566587	4.4:+67505669	None:intergenic
AGAACGGCGAAGCCGCCACC+GGG	0.567142	4.4:-67505637	MS.gene77682:CDS
TGATCTGCAGACGAAGGAGG+AGG	0.568318	4.4:+67503745	None:intergenic
AGAAGTGGATTTGGAATCGG+TGG	0.569201	4.4:+67505791	None:intergenic
CTGTACCGACCAACATGTAT+GGG	0.570736	4.4:-67503942	MS.gene77682:CDS
TTAGGAGGAGGCATAGGTGG+AGG	0.571215	4.4:+67503700	None:intergenic
TACATAGTTGATCCAGGCGG+GGG	0.571433	4.4:+67504123	None:intergenic
AGCTGGATTAGGGGCTGGTG+CGG	0.572157	4.4:-67504604	MS.gene77682:CDS
CCACAAGCTGGATTAGGGGC+TGG	0.572511	4.4:-67504609	MS.gene77682:CDS
TGAATCGGTGTTATCGGTGG+TGG	0.573466	4.4:+67505817	None:intergenic
ATTCCCCAAAACGGCATCAA+CGG	0.574006	4.4:-67505845	MS.gene77682:CDS
TACTCAGAAGAGGATGGAGA+AGG	0.574391	4.4:-67505192	MS.gene77682:CDS
CCTGGAGGTACAACAGGCTG+AGG	0.576571	4.4:+67504321	None:intergenic
GGCTTATTATTGGGCCTAGG+GGG	0.577049	4.4:-67505217	MS.gene77682:CDS
TAAGATGCAGGATATGTTGG+TGG	0.577189	4.4:+67503889	None:intergenic
GATTGGTCTGAAGTAGGGGC+AGG	0.578662	4.4:+67503814	None:intergenic
AAGGGTCGGTGAAAGAAGGT+AGG	0.578669	4.4:-67505139	MS.gene77682:CDS
CCAGCCCCTAATCCAGCTTG+TGG	0.579159	4.4:+67504609	None:intergenic
GGGGAATACTCAGAAGAGGA+TGG	0.579237	4.4:-67505198	MS.gene77682:CDS
TCATTCAAAACCTCATCCAC+AGG	0.579571	4.4:+67504993	None:intergenic
CCACCGGTATATTGTTGGGA+TGG	0.579934	4.4:+67504240	None:intergenic
CCCAGGAGGAGCACTCCCAA+TGG	0.580485	4.4:+67504212	None:intergenic
AGTATTGATTGTCCGGTGAA+GGG	0.580602	4.4:-67504798	MS.gene77682:CDS
TACCCCGGATACTCTTTCAT+CGG	0.581753	4.4:+67505251	None:intergenic
CTGCATTTCACCAGAAGCAG+GGG	0.584633	4.4:+67504014	None:intergenic
GCGGAATGATTACTGGCCCA+AGG	0.585729	4.4:+67503772	None:intergenic
GGAGGTACAACAGGCTGAGG+AGG	0.586166	4.4:+67504324	None:intergenic
TATCCATCCCAACAATATAC+CGG	0.586211	4.4:-67504243	MS.gene77682:CDS
GCTAACAATTCTGGTTCTGG+TGG	0.586625	4.4:-67504639	MS.gene77682:CDS
GAAACCCTAATTCCCCAAAA+CGG	0.588886	4.4:-67505854	MS.gene77682:CDS
ATACCGATGAAAGAGTATCC+GGG	0.589156	4.4:-67505254	MS.gene77682:CDS
CGAAGAAAAGGAAATCCAGA+TGG	0.589301	4.4:-67505613	MS.gene77682:CDS
GAGGTTGTTTGTAAGCATTG+TGG	0.589881	4.4:-67504837	MS.gene77682:CDS
TGTAAGCATTGTGGGGATGG+TGG	0.589931	4.4:-67504828	MS.gene77682:CDS
GGATACGGCTGATGCGCTTG+AGG	0.590654	4.4:-67505048	MS.gene77682:CDS
CAAACCATCATCCTCAAGAG+TGG	0.591247	4.4:+67504533	None:intergenic
ATAGGTTGACCATGCATAGG+AGG	0.592649	4.4:+67504099	None:intergenic
GAGAGTATCGTGCTCGTGAG+AGG	0.595551	4.4:-67505382	MS.gene77682:CDS
GAGGAATAGGAGGTCCCCAT+GGG	0.598071	4.4:+67504169	None:intergenic
GGTGGTAGTACACCACAAGC+TGG	0.598386	4.4:-67504621	MS.gene77682:CDS
TCATATATAGGTTCAGGTGA+AGG	0.598965	4.4:+67505431	None:intergenic
ACCGGCGGCAACACCCGAAG+CGG	0.599063	4.4:-67505752	MS.gene77682:CDS
GTGTGATTGGTCTGAAGTAG+GGG	0.599262	4.4:+67503810	None:intergenic
CATTGTGTTCCCATACATGT+TGG	0.599711	4.4:+67503933	None:intergenic
GATGAGTTAGGAGGAGGCAT+AGG	0.601322	4.4:+67503694	None:intergenic
TGGCTTTGAACAGGATAAGT+GGG	0.601464	4.4:+67504279	None:intergenic
GGGCTTATTATTGGGCCTAG+GGG	0.601483	4.4:-67505218	MS.gene77682:CDS
TAAGTGGGCACAGCCGACGC+AGG	0.602467	4.4:+67504294	None:intergenic
TGGGATTGATCTGCAGACGA+AGG	0.602817	4.4:+67503739	None:intergenic
GTGTTCCCATACATGTTGGT+CGG	0.605470	4.4:+67503937	None:intergenic
AACCTATATATGATAATATG+GGG	0.606432	4.4:-67505421	MS.gene77682:CDS
TTGTATGGAGGAGGAATAGG+AGG	0.606833	4.4:+67504159	None:intergenic
GATTGATCTGCAGACGAAGG+AGG	0.607157	4.4:+67503742	None:intergenic
GACTTCGACGTTTAAGAGTG+AGG	0.610374	4.4:-67504856	MS.gene77682:CDS
TGGGCACAGCCGACGCAGGT+GGG	0.613083	4.4:+67504298	None:intergenic
ACTATGTATCCTCCTATGCA+TGG	0.613317	4.4:-67504108	MS.gene77682:CDS
AACGACGCCGTAGAAGCCGA+TGG	0.617732	4.4:-67505685	MS.gene77682:CDS
GAACCAGAATTGTTAGCCCA+AGG	0.618282	4.4:+67504645	None:intergenic
ATACATAGTTGATCCAGGCG+GGG	0.618361	4.4:+67504122	None:intergenic
CCATCATCCTCAAGAGTGGG+TGG	0.619730	4.4:+67504537	None:intergenic
GGTGGTCTATAATCAGCAGG+TGG	0.619754	4.4:+67505299	None:intergenic
AAACCATCATCCTCAAGAGT+GGG	0.620492	4.4:+67504534	None:intergenic
GGAGTGCCTCCATAACTCCC+AGG	0.620528	4.4:+67504195	None:intergenic
GGTTGTTTGTAAGCATTGTG+GGG	0.621386	4.4:-67504835	MS.gene77682:CDS
TGCAAATCCTCATTCTCCGA+CGG	0.622653	4.4:+67505086	None:intergenic
GCAGCAACTTAGGGAGCTTG+CGG	0.623589	4.4:-67504961	MS.gene77682:CDS
GAAGAGTATTGTAGGTTGTG+TGG	0.624175	4.4:-67504915	MS.gene77682:CDS
TTTAGATGATCGTCCTGAAG+GGG	0.630920	4.4:-67505462	MS.gene77682:CDS
TGTGATTCGGGGTAAAGGGT+CGG	0.632552	4.4:-67505153	MS.gene77682:CDS
TATGCCTCCTCCTAACTCAT+CGG	0.633008	4.4:-67503692	MS.gene77682:CDS
ACTGCATTATAGTAAGGTGG+AGG	0.633851	4.4:+67503856	None:intergenic
GGAGCATAAGGATTGTATGG+AGG	0.640513	4.4:+67504147	None:intergenic
GGAGAAGCTATTGCAGCCTG+TGG	0.641988	4.4:-67505009	MS.gene77682:CDS
GAAGAAAAGGAAATCCAGAT+GGG	0.642143	4.4:-67505612	MS.gene77682:CDS
TACACCACAAGCTGGATTAG+GGG	0.643761	4.4:-67504613	MS.gene77682:CDS
GCCTCCTCCTAACTCATCGG+CGG	0.644516	4.4:-67503689	MS.gene77682:CDS
GGAACTGCATTATAGTAAGG+TGG	0.646045	4.4:+67503853	None:intergenic
TCCGAAAACGGTTTAACCGG+CGG	0.646554	4.4:-67505767	MS.gene77682:CDS
ACGACGCCGTAGAAGCCGAT+GGG	0.646571	4.4:-67505684	MS.gene77682:CDS
TATGTTGGTGGAGCTGCTGG+TGG	0.648824	4.4:+67503901	None:intergenic
TACCGATGAAAGAGTATCCG+GGG	0.650644	4.4:-67505253	MS.gene77682:CDS
TTATCAGACTGCACCCCAGG+AGG	0.652632	4.4:+67503982	None:intergenic
GGCATAGGTTGACCATGCAT+AGG	0.653836	4.4:+67504096	None:intergenic
AGCCCAATAAAATTATACCC+CGG	0.654514	4.4:+67505236	None:intergenic
TATCAGACTGCACCCCAGGA+GGG	0.665423	4.4:+67503983	None:intergenic
GGAGGCACTCCTGTCCCATG+GGG	0.666907	4.4:-67504183	MS.gene77682:CDS
CCAGAATTGTTAGCCCAAGG+AGG	0.668651	4.4:+67504648	None:intergenic
GCCGACGCAGGTGGGCCTGG+AGG	0.675462	4.4:+67504306	None:intergenic
ACGTGAATCGGTGTTATCGG+TGG	0.679354	4.4:+67505814	None:intergenic
GTTTCGGGTTTATCGTTGAG+CGG	0.681383	4.4:+67505872	None:intergenic
GCAGCAGAATTACCCTCCTG+GGG	0.683396	4.4:-67503995	MS.gene77682:CDS
TGCATTTCACCAGAAGCAGG+GGG	0.684026	4.4:+67504015	None:intergenic
CCAGATTCAAACGATCAGAA+CGG	0.685543	4.4:-67505653	MS.gene77682:CDS
GGGTATTGAACACCATAAGG+AGG	0.687098	4.4:+67504075	None:intergenic
CCATCCCAACAATATACCGG+TGG	0.690545	4.4:-67504240	MS.gene77682:CDS
ACCGACAAAGATCAATCAGG+CGG	0.691376	4.4:-67505728	MS.gene77682:CDS
GGTGCTAAGATTGTGATTCG+GGG	0.721073	4.4:-67505164	MS.gene77682:CDS
GTGCCTCCATAACTCCCAGG+AGG	0.738237	4.4:+67504198	None:intergenic
GAAGTATGCTGATATCACAA+TGG	0.751667	4.4:-67504421	MS.gene77682:CDS
GGATACATAGTTGATCCAGG+CGG	0.770511	4.4:+67504120	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AACCTATATATGATAATATG+GGG	-	chr4.4:67504108-67504127	MS.gene77682:CDS	20.0%
!!	ATATTATCATATATAGGTTC+AGG	+	chr4.4:67504107-67504126	None:intergenic	20.0%
!!	ATGATACAAATTTGTACATT+GGG	-	chr4.4:67504963-67504982	MS.gene77682:CDS	20.0%
!!	GAACCTATATATGATAATAT+GGG	-	chr4.4:67504107-67504126	MS.gene77682:CDS	20.0%
!!	TGAACCTATATATGATAATA+TGG	-	chr4.4:67504106-67504125	MS.gene77682:CDS	20.0%
!!!	AATTTTATTGGGCTTATTAT+TGG	-	chr4.4:67504302-67504321	MS.gene77682:CDS	20.0%
!!!	ATTTTATTGGGCTTATTATT+GGG	-	chr4.4:67504303-67504322	MS.gene77682:CDS	20.0%
!	ATCCAATTGTTTCAACAATT+TGG	-	chr4.4:67505019-67505038	MS.gene77682:CDS	25.0%
!	ATCCCCATATTATCATATAT+AGG	+	chr4.4:67504113-67504132	None:intergenic	25.0%
!	CTATTCAATGTTTGAATTTC+AGG	+	chr4.4:67504005-67504024	None:intergenic	25.0%
!	GATGATACAAATTTGTACAT+TGG	-	chr4.4:67504962-67504981	MS.gene77682:CDS	25.0%
!	TATATGATAATATGGGGATT+AGG	-	chr4.4:67504114-67504133	MS.gene77682:CDS	25.0%
!	TGTTATGGCAAAAGTTATTA+AGG	-	chr4.4:67505048-67505067	MS.gene77682:CDS	25.0%
	AAATCAGGAAGTTGAATTAC+CGG	+	chr4.4:67503960-67503979	None:intergenic	30.0%
	AATTCAACTTCCTGATTTCA+TGG	-	chr4.4:67503962-67503981	MS.gene77682:CDS	30.0%
	ATAAGTAGAATGTTGCAATC+TGG	-	chr4.4:67504038-67504057	MS.gene77682:CDS	30.0%
	ATTCAACTTCCTGATTTCAT+GGG	-	chr4.4:67503963-67503982	MS.gene77682:CDS	30.0%
	CACCAAATTGTTGAAACAAT+TGG	+	chr4.4:67505024-67505043	None:intergenic	30.0%
	GATTAAGACAACTAGTACTT+TGG	-	chr4.4:67504829-67504848	MS.gene77682:CDS	30.0%
	TGATGAGTATCAGAATTTCT+TGG	-	chr4.4:67504775-67504794	MS.gene77682:CDS	30.0%
	TTCTGAGTATGAGAAATTCA+TGG	-	chr4.4:67505885-67505904	MS.gene77682:CDS	30.0%
!	ACAATTTGGTGAGATTGTTA+TGG	-	chr4.4:67505033-67505052	MS.gene77682:CDS	30.0%
!	ATCCGGGGTATAATTTTATT+GGG	-	chr4.4:67504291-67504310	MS.gene77682:CDS	30.0%
!	TATCCGGGGTATAATTTTAT+TGG	-	chr4.4:67504290-67504309	MS.gene77682:CDS	30.0%
	AATGCAATTGTTGCCATGAA+TGG	-	chr4.4:67505136-67505155	MS.gene77682:CDS	35.0%
	ACAACTAGTACTTTGGCAAT+TGG	-	chr4.4:67504836-67504855	MS.gene77682:CDS	35.0%
	ACAGGTTTGAGTAAAGGATA+CGG	-	chr4.4:67505079-67505098	MS.gene77682:CDS	35.0%
	AGCCCAATAAAATTATACCC+CGG	+	chr4.4:67504296-67504315	None:intergenic	35.0%
	GAAGAAAAGGAAATCCAGAT+GGG	-	chr4.4:67503917-67503936	MS.gene77682:CDS	35.0%
	GAAGTATGCTGATATCACAA+TGG	-	chr4.4:67505108-67505127	MS.gene77682:CDS	35.0%
	GTTATTAAGGATCGAATGAC+AGG	-	chr4.4:67505061-67505080	MS.gene77682:CDS	35.0%
	TAGTACTTTGGCAATTGGTT+CGG	-	chr4.4:67504841-67504860	MS.gene77682:CDS	35.0%
	TATCCATCCCAACAATATAC+CGG	-	chr4.4:67505286-67505305	MS.gene77682:CDS	35.0%
	TCATATATAGGTTCAGGTGA+AGG	+	chr4.4:67504101-67504120	None:intergenic	35.0%
!	AGGTTGTTTGTAAGCATTGT+GGG	-	chr4.4:67504693-67504712	MS.gene77682:CDS	35.0%
!	GAATGAGGATTTGCATGTTT+TGG	-	chr4.4:67504451-67504470	MS.gene77682:CDS	35.0%
!!	TAAAGCTTCTTCTGAAGCTT+TGG	+	chr4.4:67504254-67504273	None:intergenic	35.0%
!!	TATACCGATGAAAGAGTATC+CGG	-	chr4.4:67504274-67504293	MS.gene77682:CDS	35.0%
!!	TCATCCTAGTATTGATTGTC+CGG	-	chr4.4:67504724-67504743	MS.gene77682:CDS	35.0%
!!	TTAGGGATGAAGAGTATTGT+AGG	-	chr4.4:67504606-67504625	MS.gene77682:CDS	35.0%
!!!	AGGTTTTGAATGAGCATAAG+AGG	-	chr4.4:67504546-67504565	MS.gene77682:CDS	35.0%
	AAACCATCATCCTCAAGAGT+GGG	+	chr4.4:67504998-67505017	None:intergenic	40.0%
	AATCCACTTCTCTCCGAAAA+CGG	-	chr4.4:67503750-67503769	MS.gene77682:CDS	40.0%
	ACTATGTATCCTCCTATGCA+TGG	-	chr4.4:67505421-67505440	MS.gene77682:CDS	40.0%
	ACTATTGCAGTAAGAGTTGC+TGG	-	chr4.4:67505178-67505197	MS.gene77682:CDS	40.0%
	ACTGCATTATAGTAAGGTGG+AGG	+	chr4.4:67505676-67505695	None:intergenic	40.0%
	CAGAATTTCTTGGCTGAGTT+GGG	-	chr4.4:67504785-67504804	MS.gene77682:CDS	40.0%
	CAGGTTTGAGTAAAGGATAC+GGG	-	chr4.4:67505080-67505099	MS.gene77682:CDS	40.0%
	CATTGTGTTCCCATACATGT+TGG	+	chr4.4:67505599-67505618	None:intergenic	40.0%
	CATTTGCAATGCTGTGTGAT+TGG	+	chr4.4:67505735-67505754	None:intergenic	40.0%
	CCAACATATCCTGCATCTTA+TGG	-	chr4.4:67505643-67505662	MS.gene77682:CDS	40.0%
	CCAGATTCAAACGATCAGAA+CGG	-	chr4.4:67503876-67503895	MS.gene77682:CDS	40.0%
	CCATAAGATGCAGGATATGT+TGG	+	chr4.4:67505646-67505665	None:intergenic	40.0%
	CGAAGAAAAGGAAATCCAGA+TGG	-	chr4.4:67503916-67503935	MS.gene77682:CDS	40.0%
	CGAATGACAGGTTTGAGTAA+AGG	-	chr4.4:67505073-67505092	MS.gene77682:CDS	40.0%
	CTTTAGATGATCGTCCTGAA+GGG	-	chr4.4:67504066-67504085	MS.gene77682:CDS	40.0%
	GAAACCCTAATTCCCCAAAA+CGG	-	chr4.4:67503675-67503694	MS.gene77682:CDS	40.0%
	GGAACTGCATTATAGTAAGG+TGG	+	chr4.4:67505679-67505698	None:intergenic	40.0%
	GGAGGAACTGCATTATAGTA+AGG	+	chr4.4:67505682-67505701	None:intergenic	40.0%
	GGTATATTGTTGGGATGGAT+AGG	+	chr4.4:67505287-67505306	None:intergenic	40.0%
	GTTGTCTTAATCGCAGATTC+AGG	+	chr4.4:67504821-67504840	None:intergenic	40.0%
	TAAGATGCAGGATATGTTGG+TGG	+	chr4.4:67505643-67505662	None:intergenic	40.0%
	TCAATACCACCCATGAAATC+AGG	+	chr4.4:67503975-67503994	None:intergenic	40.0%
	TCAGAATTTCTTGGCTGAGT+TGG	-	chr4.4:67504784-67504803	MS.gene77682:CDS	40.0%
	TCATTCAAAACCTCATCCAC+AGG	+	chr4.4:67504539-67504558	None:intergenic	40.0%
	TTAAACGTCGAAGTCCTTGT+AGG	+	chr4.4:67504668-67504687	None:intergenic	40.0%
	TTCACCGGACAATCAATACT+AGG	+	chr4.4:67504731-67504750	None:intergenic	40.0%
	TTTAGATGATCGTCCTGAAG+GGG	-	chr4.4:67504067-67504086	MS.gene77682:CDS	40.0%
!	AAGATTGTGATTCGGGGTAA+AGG	-	chr4.4:67504371-67504390	MS.gene77682:CDS	40.0%
!	AGATTGTGATTCGGGGTAAA+GGG	-	chr4.4:67504372-67504391	MS.gene77682:CDS	40.0%
!	AGTACTTTGGCAATTGGTTC+GGG	-	chr4.4:67504842-67504861	MS.gene77682:CDS	40.0%
!	CTGGTGCTAAGATTGTGATT+CGG	-	chr4.4:67504363-67504382	MS.gene77682:CDS	40.0%
!	GAGAGAAGTGGATTTGGAAT+CGG	+	chr4.4:67503744-67503763	None:intergenic	40.0%
!	GAGGTTGTTTGTAAGCATTG+TGG	-	chr4.4:67504692-67504711	MS.gene77682:CDS	40.0%
!	GGTTGTTTGTAAGCATTGTG+GGG	-	chr4.4:67504694-67504713	MS.gene77682:CDS	40.0%
!	TGGTGCTAAGATTGTGATTC+GGG	-	chr4.4:67504364-67504383	MS.gene77682:CDS	40.0%
!	TTTGGTGGTCTATAATCAGC+AGG	+	chr4.4:67504236-67504255	None:intergenic	40.0%
!!	AGTATTGATTGTCCGGTGAA+GGG	-	chr4.4:67504731-67504750	MS.gene77682:CDS	40.0%
!!	ATACCGATGAAAGAGTATCC+GGG	-	chr4.4:67504275-67504294	MS.gene77682:CDS	40.0%
!!	GAAGAGTATTGTAGGTTGTG+TGG	-	chr4.4:67504614-67504633	MS.gene77682:CDS	40.0%
!!	GGAAGTTGAATTACCGGTTT+CGG	+	chr4.4:67503954-67503973	None:intergenic	40.0%
!!	TAGTATTGATTGTCCGGTGA+AGG	-	chr4.4:67504730-67504749	MS.gene77682:CDS	40.0%
!!	TGGCTTTGAACAGGATAAGT+GGG	+	chr4.4:67505253-67505272	None:intergenic	40.0%
!!	TGTGTGATTGGTCTGAAGTA+GGG	+	chr4.4:67505723-67505742	None:intergenic	40.0%
!!	TTTTCGGAGAGAAGTGGATT+TGG	+	chr4.4:67503750-67503769	None:intergenic	40.0%
!!	TTTTGGGGAATTAGGGTTTC+GGG	+	chr4.4:67503675-67503694	None:intergenic	40.0%
!!!	GGATTTGCATGTTTTGGTTG+AGG	-	chr4.4:67504457-67504476	MS.gene77682:CDS	40.0%
!!!	GTTTTGGGGAATTAGGGTTT+CGG	+	chr4.4:67503676-67503695	None:intergenic	40.0%
!!!	TTTGCATGTTTTGGTTGAGG+CGG	-	chr4.4:67504460-67504479	MS.gene77682:CDS	40.0%
	ACCGACAAAGATCAATCAGG+CGG	-	chr4.4:67503801-67503820	MS.gene77682:CDS	45.0%
	AGCATAAGAGGCAGCAACTT+AGG	-	chr4.4:67504558-67504577	MS.gene77682:CDS	45.0%
	AGGCACATTTCCAATGCTCT+GGG	+	chr4.4:67505812-67505831	None:intergenic	45.0%
	AGTACACCACAAGCTGGATT+AGG	-	chr4.4:67504914-67504933	MS.gene77682:CDS	45.0%
	ATACATAGTTGATCCAGGCG+GGG	+	chr4.4:67505410-67505429	None:intergenic	45.0%
	ATGAATGGCTATCGCCTTGA+GGG	-	chr4.4:67505151-67505170	MS.gene77682:CDS	45.0%
	ATTCCCCAAAACGGCATCAA+CGG	-	chr4.4:67503684-67503703	MS.gene77682:CDS	45.0%
	CAAACCATCATCCTCAAGAG+TGG	+	chr4.4:67504999-67505018	None:intergenic	45.0%
	CAACTTCCTGATTTCATGGG+TGG	-	chr4.4:67503966-67503985	MS.gene77682:CDS	45.0%
	CCGTTCTGATCGTTTGAATC+TGG	+	chr4.4:67503879-67503898	None:intergenic	45.0%
	CCTTCAGGACGATCATCTAA+AGG	+	chr4.4:67504068-67504087	None:intergenic	45.0%
	CCTTTAGATGATCGTCCTGA+AGG	-	chr4.4:67504065-67504084	MS.gene77682:CDS	45.0%
	CTCTCCGAAAACGGTTTAAC+CGG	-	chr4.4:67503759-67503778	MS.gene77682:CDS	45.0%
	GAACCAGAATTGTTAGCCCA+AGG	+	chr4.4:67504887-67504906	None:intergenic	45.0%
	GACTTCGACGTTTAAGAGTG+AGG	-	chr4.4:67504673-67504692	MS.gene77682:CDS	45.0%
	GATACATAGTTGATCCAGGC+GGG	+	chr4.4:67505411-67505430	None:intergenic	45.0%
	GATCGTTTGAATCTGGCTGT+GGG	+	chr4.4:67503872-67503891	None:intergenic	45.0%
	GCATAAGAGGCAGCAACTTA+GGG	-	chr4.4:67504559-67504578	MS.gene77682:CDS	45.0%
	GCATAAGGATTGTATGGAGG+AGG	+	chr4.4:67505382-67505401	None:intergenic	45.0%
	GCATTATAGTAAGGTGGAGG+AGG	+	chr4.4:67505673-67505692	None:intergenic	45.0%
	GGAACCGACAAAGATCAATC+AGG	-	chr4.4:67503798-67503817	MS.gene77682:CDS	45.0%
	GGAGCATAAGGATTGTATGG+AGG	+	chr4.4:67505385-67505404	None:intergenic	45.0%
	GGAGGATACATAGTTGATCC+AGG	+	chr4.4:67505415-67505434	None:intergenic	45.0%
	GGAGGATATCCATAAGATGC+AGG	+	chr4.4:67505655-67505674	None:intergenic	45.0%
	GGATACATAGTTGATCCAGG+CGG	+	chr4.4:67505412-67505431	None:intergenic	45.0%
	GGATTGTATGGAGGAGGAAT+AGG	+	chr4.4:67505376-67505395	None:intergenic	45.0%
	GGTGCTAAGATTGTGATTCG+GGG	-	chr4.4:67504365-67504384	MS.gene77682:CDS	45.0%
	GTACACCACAAGCTGGATTA+GGG	-	chr4.4:67504915-67504934	MS.gene77682:CDS	45.0%
	GTGTTCCCATACATGTTGGT+CGG	+	chr4.4:67505595-67505614	None:intergenic	45.0%
	TACCCCGGATACTCTTTCAT+CGG	+	chr4.4:67504281-67504300	None:intergenic	45.0%
	TACTCAGAAGAGGATGGAGA+AGG	-	chr4.4:67504337-67504356	MS.gene77682:CDS	45.0%
	TATGCCTCCTCCTAACTCAT+CGG	-	chr4.4:67505837-67505856	MS.gene77682:CDS	45.0%
	TCCGCCTGATTGATCTTTGT+CGG	+	chr4.4:67503805-67503824	None:intergenic	45.0%
	TGATCGTTTGAATCTGGCTG+TGG	+	chr4.4:67503873-67503892	None:intergenic	45.0%
	TGCAAATCCTCATTCTCCGA+CGG	+	chr4.4:67504446-67504465	None:intergenic	45.0%
	TGGGCTTATTATTGGGCCTA+GGG	-	chr4.4:67504310-67504329	MS.gene77682:CDS	45.0%
	TTATTATCAGACTGCACCCC+AGG	+	chr4.4:67505553-67505572	None:intergenic	45.0%
	TTGGGCTTATTATTGGGCCT+AGG	-	chr4.4:67504309-67504328	MS.gene77682:CDS	45.0%
	TTGTATGGAGGAGGAATAGG+AGG	+	chr4.4:67505373-67505392	None:intergenic	45.0%
!	AAACCGTTTTCGGAGAGAAG+TGG	+	chr4.4:67503756-67503775	None:intergenic	45.0%
!	AATTTCTTGGCTGAGTTGGG+TGG	-	chr4.4:67504788-67504807	MS.gene77682:CDS	45.0%
!	AGAAGTGGATTTGGAATCGG+TGG	+	chr4.4:67503741-67503760	None:intergenic	45.0%
!	ATAGGTTGACCATGCATAGG+AGG	+	chr4.4:67505433-67505452	None:intergenic	45.0%
!	CTGTACCGACCAACATGTAT+GGG	-	chr4.4:67505587-67505606	MS.gene77682:CDS	45.0%
!	GCAGAAGAGGGATTTGAAAC+CGG	-	chr4.4:67504418-67504437	MS.gene77682:CDS	45.0%
!	GGAGGAGCATAAGGATTGTA+TGG	+	chr4.4:67505388-67505407	None:intergenic	45.0%
!	GGATTTCCTTTTCTTCGTCC+CGG	+	chr4.4:67503913-67503932	None:intergenic	45.0%
!	GTTTGTAAGCATTGTGGGGA+TGG	-	chr4.4:67504698-67504717	MS.gene77682:CDS	45.0%
!	TACACCACAAGCTGGATTAG+GGG	-	chr4.4:67504916-67504935	MS.gene77682:CDS	45.0%
!	TCTGTACCGACCAACATGTA+TGG	-	chr4.4:67505586-67505605	MS.gene77682:CDS	45.0%
!	TGAACACCATAAGGAGGCAT+AGG	+	chr4.4:67505451-67505470	None:intergenic	45.0%
!	TTCTGGAAGCAATCCTCCTT+GGG	-	chr4.4:67504868-67504887	MS.gene77682:CDS	45.0%
!!	AGCTTCTTCTGAAGCTTTGG+TGG	+	chr4.4:67504251-67504270	None:intergenic	45.0%
!!	CTGGCTTTGAACAGGATAAG+TGG	+	chr4.4:67505254-67505273	None:intergenic	45.0%
!!	CTGTGTGATTGGTCTGAAGT+AGG	+	chr4.4:67505724-67505743	None:intergenic	45.0%
!!	GCTAACAATTCTGGTTCTGG+TGG	-	chr4.4:67504890-67504909	MS.gene77682:CDS	45.0%
!!	GGCTTTGAATGGGACGATTA+GGG	-	chr4.4:67504589-67504608	MS.gene77682:CDS	45.0%
!!	GGGTATTGAACACCATAAGG+AGG	+	chr4.4:67505457-67505476	None:intergenic	45.0%
!!	GGTGGGTATTGAACACCATA+AGG	+	chr4.4:67505460-67505479	None:intergenic	45.0%
!!	GTGTGATTGGTCTGAAGTAG+GGG	+	chr4.4:67505722-67505741	None:intergenic	45.0%
!!	GTTTCGGGTTTATCGTTGAG+CGG	+	chr4.4:67503660-67503679	None:intergenic	45.0%
!!	TACCGATGAAAGAGTATCCG+GGG	-	chr4.4:67504276-67504295	MS.gene77682:CDS	45.0%
!!	TGGACGTGAATCGGTGTTAT+CGG	+	chr4.4:67503721-67503740	None:intergenic	45.0%
!!	TGGGCTAACAATTCTGGTTC+TGG	-	chr4.4:67504887-67504906	MS.gene77682:CDS	45.0%
!!!	GATGCCGTTTTGGGGAATTA+GGG	+	chr4.4:67503682-67503701	None:intergenic	45.0%
!!!	TGATGCCGTTTTGGGGAATT+AGG	+	chr4.4:67503683-67503702	None:intergenic	45.0%
!!!	TGTTTTGGTTGAGGCGGATA+CGG	-	chr4.4:67504466-67504485	MS.gene77682:CDS	45.0%
	AAGAGGATGGAGAAGGAGAC+TGG	-	chr4.4:67504344-67504363	MS.gene77682:CDS	50.0%
	AAGGGTCGGTGAAAGAAGGT+AGG	-	chr4.4:67504390-67504409	MS.gene77682:CDS	50.0%
	AAGGTAGGTTGCAGCAGAAG+AGG	-	chr4.4:67504405-67504424	MS.gene77682:CDS	50.0%
	ACAGCATTGCAAATGCGCCT+TGG	-	chr4.4:67505740-67505759	MS.gene77682:CDS	50.0%
	ACCGCCACCGGTATATTGTT+GGG	+	chr4.4:67505296-67505315	None:intergenic	50.0%
	AGGTAGGTTGCAGCAGAAGA+GGG	-	chr4.4:67504406-67504425	MS.gene77682:CDS	50.0%
	ATGCAGCAGAATTACCCTCC+TGG	-	chr4.4:67505532-67505551	MS.gene77682:CDS	50.0%
	ATGGATAGGCACCAATTGGC+TGG	+	chr4.4:67505273-67505292	None:intergenic	50.0%
	CAGCATTGCAAATGCGCCTT+GGG	-	chr4.4:67505741-67505760	MS.gene77682:CDS	50.0%
	CATGAATGGCTATCGCCTTG+AGG	-	chr4.4:67505150-67505169	MS.gene77682:CDS	50.0%
	CCACCGGTATATTGTTGGGA+TGG	+	chr4.4:67505292-67505311	None:intergenic	50.0%
	CCAGAATTGTTAGCCCAAGG+AGG	+	chr4.4:67504884-67504903	None:intergenic	50.0%
	CCATCCCAACAATATACCGG+TGG	-	chr4.4:67505289-67505308	MS.gene77682:CDS	50.0%
	CCTCCTTGGGCTAACAATTC+TGG	-	chr4.4:67504881-67504900	MS.gene77682:CDS	50.0%
	CCTGTTCAAAGCCAGCCAAT+TGG	-	chr4.4:67505259-67505278	MS.gene77682:CDS	50.0%
	CTAGGGGGAATACTCAGAAG+AGG	-	chr4.4:67504327-67504346	MS.gene77682:CDS	50.0%
	CTCAAGGCGATAGCCATTCA+TGG	+	chr4.4:67505152-67505171	None:intergenic	50.0%
	CTCTTCTGAGTATTCCCCCT+AGG	+	chr4.4:67504329-67504348	None:intergenic	50.0%
	CTGCAATAGTTCGACCCTCA+AGG	+	chr4.4:67505168-67505187	None:intergenic	50.0%
	CTGCATTTCACCAGAAGCAG+GGG	+	chr4.4:67505518-67505537	None:intergenic	50.0%
	GAGGCACATTTCCAATGCTC+TGG	+	chr4.4:67505813-67505832	None:intergenic	50.0%
	GATTGATCTGCAGACGAAGG+AGG	+	chr4.4:67505790-67505809	None:intergenic	50.0%
	GCAGATCAATCCCAGAGCAT+TGG	-	chr4.4:67505799-67505818	MS.gene77682:CDS	50.0%
	GCTGCATTTCACCAGAAGCA+GGG	+	chr4.4:67505519-67505538	None:intergenic	50.0%
	GGCTTATTATTGGGCCTAGG+GGG	-	chr4.4:67504312-67504331	MS.gene77682:CDS	50.0%
	GGGCTTATTATTGGGCCTAG+GGG	-	chr4.4:67504311-67504330	MS.gene77682:CDS	50.0%
	GGGGAATACTCAGAAGAGGA+TGG	-	chr4.4:67504331-67504350	MS.gene77682:CDS	50.0%
	GGTAAAGGGTCGGTGAAAGA+AGG	-	chr4.4:67504386-67504405	MS.gene77682:CDS	50.0%
	GGTCAACCTATGCCTCCTTA+TGG	-	chr4.4:67505442-67505461	MS.gene77682:CDS	50.0%
	GGTGGTCTATAATCAGCAGG+TGG	+	chr4.4:67504233-67504252	None:intergenic	50.0%
	GGTTTATCGTTGAGCGGTAG+TGG	+	chr4.4:67503654-67503673	None:intergenic	50.0%
	GTATTTGCCACCCACTCTTG+AGG	-	chr4.4:67504985-67505004	MS.gene77682:CDS	50.0%
	TACATAGTTGATCCAGGCGG+GGG	+	chr4.4:67505409-67505428	None:intergenic	50.0%
	TCAGCAGGTGGCTTAAAAGC+AGG	+	chr4.4:67504221-67504240	None:intergenic	50.0%
	TCCCAACAATATACCGGTGG+CGG	-	chr4.4:67505292-67505311	MS.gene77682:CDS	50.0%
	TCCGAAAACGGTTTAACCGG+CGG	-	chr4.4:67503762-67503781	MS.gene77682:CDS	50.0%
	TCGGCGGAAAAGACGTCATA+TGG	-	chr4.4:67505856-67505875	MS.gene77682:CDS	50.0%
	TGCAGCAGAATTACCCTCCT+GGG	-	chr4.4:67505533-67505552	MS.gene77682:CDS	50.0%
	TGCATTTCACCAGAAGCAGG+GGG	+	chr4.4:67505517-67505536	None:intergenic	50.0%
	TGCTGCATTTCACCAGAAGC+AGG	+	chr4.4:67505520-67505539	None:intergenic	50.0%
	TGGAATCGGTGGACGTGAAT+CGG	+	chr4.4:67503730-67503749	None:intergenic	50.0%
	TGGGATGGATAGGCACCAAT+TGG	+	chr4.4:67505277-67505296	None:intergenic	50.0%
	TGGGATTGATCTGCAGACGA+AGG	+	chr4.4:67505793-67505812	None:intergenic	50.0%
!	GATGAGTTAGGAGGAGGCAT+AGG	+	chr4.4:67505838-67505857	None:intergenic	50.0%
!	GGATTTGAAACCGGATCCGT+CGG	-	chr4.4:67504427-67504446	MS.gene77682:CDS	50.0%
!	GGCATAGGTTGACCATGCAT+AGG	+	chr4.4:67505436-67505455	None:intergenic	50.0%
!	GTCTTTTCCGCCGATGAGTT+AGG	+	chr4.4:67505850-67505869	None:intergenic	50.0%
!	TGTAAGCATTGTGGGGATGG+TGG	-	chr4.4:67504701-67504720	MS.gene77682:CDS	50.0%
!	TGTGATTCGGGGTAAAGGGT+CGG	-	chr4.4:67504376-67504395	MS.gene77682:CDS	50.0%
!	TTTCCTTTTCTTCGTCCCGG+TGG	+	chr4.4:67503910-67503929	None:intergenic	50.0%
!	TTTTCCGCCGATGAGTTAGG+AGG	+	chr4.4:67505847-67505866	None:intergenic	50.0%
!!	ACGTGAATCGGTGTTATCGG+TGG	+	chr4.4:67503718-67503737	None:intergenic	50.0%
!!	ATCGTTGAGCGGTAGTGGAT+TGG	+	chr4.4:67503649-67503668	None:intergenic	50.0%
!!	ATCTTTGTCGGTTCCGCTTC+GGG	+	chr4.4:67503793-67503812	None:intergenic	50.0%
!!	CCAATTGGCTGGCTTTGAAC+AGG	+	chr4.4:67505262-67505281	None:intergenic	50.0%
!!	CGGCTTTGAATGGGACGATT+AGG	-	chr4.4:67504588-67504607	MS.gene77682:CDS	50.0%
!!	GATCTTTGTCGGTTCCGCTT+CGG	+	chr4.4:67503794-67503813	None:intergenic	50.0%
!!	GTTCTGGAAGCAATCCTCCT+TGG	-	chr4.4:67504867-67504886	MS.gene77682:CDS	50.0%
!!	TGAATCGGTGTTATCGGTGG+TGG	+	chr4.4:67503715-67503734	None:intergenic	50.0%
	AATCTGGCTGTGGGTCCCAT+CGG	+	chr4.4:67503863-67503882	None:intergenic	55.0%
	AGGAGGGGCGGAATGATTAC+TGG	+	chr4.4:67505767-67505786	None:intergenic	55.0%
	AGTGCTCCTCCTGGGAGTTA+TGG	-	chr4.4:67505325-67505344	MS.gene77682:CDS	55.0%
	ATTTCACCAGAAGCAGGGGG+TGG	+	chr4.4:67505514-67505533	None:intergenic	55.0%
	CCACCCACTCTTGAGGATGA+TGG	-	chr4.4:67504992-67505011	MS.gene77682:CDS	55.0%
	CCATCATCCTCAAGAGTGGG+TGG	+	chr4.4:67504995-67505014	None:intergenic	55.0%
	GAAGGAGATCTAGCCCCTTC+AGG	+	chr4.4:67504083-67504102	None:intergenic	55.0%
	GACCGCCACCGGTATATTGT+TGG	+	chr4.4:67505297-67505316	None:intergenic	55.0%
	GAGGAATAGGAGGTCCCCAT+GGG	+	chr4.4:67505363-67505382	None:intergenic	55.0%
	GCAGCAACTTAGGGAGCTTG+CGG	-	chr4.4:67504568-67504587	MS.gene77682:CDS	55.0%
	GCAGCAGAATTACCCTCCTG+GGG	-	chr4.4:67505534-67505553	MS.gene77682:CDS	55.0%
	GCGGAATGATTACTGGCCCA+AGG	+	chr4.4:67505760-67505779	None:intergenic	55.0%
	GCTATTGCAGCCTGTGGATG+AGG	-	chr4.4:67504526-67504545	MS.gene77682:CDS	55.0%
	GGAGAAGCTATTGCAGCCTG+TGG	-	chr4.4:67504520-67504539	MS.gene77682:CDS	55.0%
	GGCAGTATGCGTGTCCTACA+AGG	-	chr4.4:67504651-67504670	MS.gene77682:CDS	55.0%
	GGTGGTAGTACACCACAAGC+TGG	-	chr4.4:67504908-67504927	MS.gene77682:CDS	55.0%
	GTAGGTTGTGTGGTGAGCCT+GGG	-	chr4.4:67504624-67504643	MS.gene77682:CDS	55.0%
	TATCAGACTGCACCCCAGGA+GGG	+	chr4.4:67505549-67505568	None:intergenic	55.0%
	TCCTCATTCTCCGACGGATC+CGG	+	chr4.4:67504440-67504459	None:intergenic	55.0%
	TGATCTGCAGACGAAGGAGG+AGG	+	chr4.4:67505787-67505806	None:intergenic	55.0%
	TGTAGGTTGTGTGGTGAGCC+TGG	-	chr4.4:67504623-67504642	MS.gene77682:CDS	55.0%
	TTATCAGACTGCACCCCAGG+AGG	+	chr4.4:67505550-67505569	None:intergenic	55.0%
	TTCCCAGTAGCACCCTTCAC+CGG	+	chr4.4:67504746-67504765	None:intergenic	55.0%
	TTTCACCAGAAGCAGGGGGT+GGG	+	chr4.4:67505513-67505532	None:intergenic	55.0%
!	AGGGGCTGGTGCGGTTAAAA+AGG	-	chr4.4:67504934-67504953	MS.gene77682:CDS	55.0%
!	GAGTTAGGAGGAGGCATAGG+TGG	+	chr4.4:67505835-67505854	None:intergenic	55.0%
!	GGATATGTTGGTGGAGCTGC+TGG	+	chr4.4:67505634-67505653	None:intergenic	55.0%
!	GGCTGATGACGAACCGAAAC+CGG	-	chr4.4:67503938-67503957	MS.gene77682:CDS	55.0%
!	TATGTTGGTGGAGCTGCTGG+TGG	+	chr4.4:67505631-67505650	None:intergenic	55.0%
!	TTAGGAGGAGGCATAGGTGG+AGG	+	chr4.4:67505832-67505851	None:intergenic	55.0%
!!	AGGGAGCTTGCGGCTTTGAA+TGG	-	chr4.4:67504578-67504597	MS.gene77682:CDS	55.0%
!!	ATCGGTGTTATCGGTGGTGG+TGG	+	chr4.4:67503712-67503731	None:intergenic	55.0%
!!	GAGAGTATCGTGCTCGTGAG+AGG	-	chr4.4:67504147-67504166	MS.gene77682:CDS	55.0%
!!	GATTGGTCTGAAGTAGGGGC+AGG	+	chr4.4:67505718-67505737	None:intergenic	55.0%
!!	GCAATTGGTTCGGGCAGTTC+TGG	-	chr4.4:67504851-67504870	MS.gene77682:CDS	55.0%
!!	GCCGCCGGTTAAACCGTTTT+CGG	+	chr4.4:67503766-67503785	None:intergenic	55.0%
!!	GGGAGCTTGCGGCTTTGAAT+GGG	-	chr4.4:67504579-67504598	MS.gene77682:CDS	55.0%
!!	GGGTGCTACTGGGAAGAAGA+TGG	-	chr4.4:67504751-67504770	MS.gene77682:CDS	55.0%
!!!	GGTGCCGTTGATGCCGTTTT+GGG	+	chr4.4:67503691-67503710	None:intergenic	55.0%
!!!	GTGCCGTTGATGCCGTTTTG+GGG	+	chr4.4:67503690-67503709	None:intergenic	55.0%
!!!	TGGTGCCGTTGATGCCGTTT+TGG	+	chr4.4:67503692-67503711	None:intergenic	55.0%
	AACGACGCCGTAGAAGCCGA+TGG	-	chr4.4:67503844-67503863	MS.gene77682:CDS	60.0%
	ACCGGATCCGTCGGAGAATG+AGG	-	chr4.4:67504436-67504455	MS.gene77682:CDS	60.0%
	ACGACGCCGTAGAAGCCGAT+GGG	-	chr4.4:67503845-67503864	MS.gene77682:CDS	60.0%
	ATAGGAGGTCCCCATGGGAC+AGG	+	chr4.4:67505358-67505377	None:intergenic	60.0%
	ATAGTTGATCCAGGCGGGGG+AGG	+	chr4.4:67505406-67505425	None:intergenic	60.0%
	CAATACCCACCCATGCCGAC+AGG	-	chr4.4:67505469-67505488	MS.gene77682:CDS	60.0%
	CCAGCCCCTAATCCAGCTTG+TGG	+	chr4.4:67504923-67504942	None:intergenic	60.0%
	CCTCAGCCTGTTGTACCTCC+AGG	-	chr4.4:67505208-67505227	MS.gene77682:CDS	60.0%
	CGGTTCGTCATCAGCCCATC+TGG	+	chr4.4:67503934-67503953	None:intergenic	60.0%
	CTGCAGACGAAGGAGGAGGA+GGG	+	chr4.4:67505783-67505802	None:intergenic	60.0%
	GCCTCCTCCTAACTCATCGG+CGG	-	chr4.4:67505840-67505859	MS.gene77682:CDS	60.0%
	GCTCCTCCTGGGAGTTATGG+AGG	-	chr4.4:67505328-67505347	MS.gene77682:CDS	60.0%
	GGAGGAATAGGAGGTCCCCA+TGG	+	chr4.4:67505364-67505383	None:intergenic	60.0%
	GGATACGGCTGATGCGCTTG+AGG	-	chr4.4:67504481-67504500	MS.gene77682:CDS	60.0%
	GTGCCTCCATAACTCCCAGG+AGG	+	chr4.4:67505334-67505353	None:intergenic	60.0%
	TCCGCCGATGAGTTAGGAGG+AGG	+	chr4.4:67505844-67505863	None:intergenic	60.0%
	TCTGCAGACGAAGGAGGAGG+AGG	+	chr4.4:67505784-67505803	None:intergenic	60.0%
	TGCAGACGAAGGAGGAGGAG+GGG	+	chr4.4:67505782-67505801	None:intergenic	60.0%
	TGTGTGGTGAGCCTGGGCAT+CGG	-	chr4.4:67504630-67504649	MS.gene77682:CDS	60.0%
	TTCACCAGAAGCAGGGGGTG+GGG	+	chr4.4:67505512-67505531	None:intergenic	60.0%
!	AGCTGGATTAGGGGCTGGTG+CGG	-	chr4.4:67504925-67504944	MS.gene77682:CDS	60.0%
!	ATACCGGTGGCGGTCCCATT+GGG	-	chr4.4:67505302-67505321	MS.gene77682:CDS	60.0%
!	CCACAAGCTGGATTAGGGGC+TGG	-	chr4.4:67504920-67504939	MS.gene77682:CDS	60.0%
!	CCTGGAGGTACAACAGGCTG+AGG	+	chr4.4:67505211-67505230	None:intergenic	60.0%
!	CCTTTTCTTCGTCCCGGTGG+CGG	+	chr4.4:67503907-67503926	None:intergenic	60.0%
!	GGAGCTGCTGGTGGCATTGA+AGG	+	chr4.4:67505622-67505641	None:intergenic	60.0%
!	GGAGGTACAACAGGCTGAGG+AGG	+	chr4.4:67505208-67505227	None:intergenic	60.0%
!	GGAGTGCCTCCATAACTCCC+AGG	+	chr4.4:67505337-67505356	None:intergenic	60.0%
!	GGTCTGAAGTAGGGGCAGGA+GGG	+	chr4.4:67505714-67505733	None:intergenic	60.0%
!	GTCTGAAGTAGGGGCAGGAG+GGG	+	chr4.4:67505713-67505732	None:intergenic	60.0%
!	TATACCGGTGGCGGTCCCAT+TGG	-	chr4.4:67505301-67505320	MS.gene77682:CDS	60.0%
!	TCTGAAGTAGGGGCAGGAGG+GGG	+	chr4.4:67505712-67505731	None:intergenic	60.0%
!	TGGTCTGAAGTAGGGGCAGG+AGG	+	chr4.4:67505715-67505734	None:intergenic	60.0%
!!	ATGGAGGCACTCCTGTCCCA+TGG	-	chr4.4:67505344-67505363	MS.gene77682:CDS	60.0%
!!	CCAGGAGGAGCACTCCCAAT+GGG	+	chr4.4:67505319-67505338	None:intergenic	60.0%
!!	CCATTGGGAGTGCTCCTCCT+GGG	-	chr4.4:67505317-67505336	MS.gene77682:CDS	60.0%
!!	GTCCGGTGAAGGGTGCTACT+GGG	-	chr4.4:67504741-67504760	MS.gene77682:CDS	60.0%
!!	GTGGGTCCCATCGGCTTCTA+CGG	+	chr4.4:67503854-67503873	None:intergenic	60.0%
!!	TGGAGGCACTCCTGTCCCAT+GGG	-	chr4.4:67505345-67505364	MS.gene77682:CDS	60.0%
!!	TGTCCGGTGAAGGGTGCTAC+TGG	-	chr4.4:67504740-67504759	MS.gene77682:CDS	60.0%
	ACTCCCAATGGGACCGCCAC+CGG	+	chr4.4:67505308-67505327	None:intergenic	65.0%
	AGACGAAGGAGGAGGAGGGG+CGG	+	chr4.4:67505779-67505798	None:intergenic	65.0%
	CACGCATACTGCCGATGCCC+AGG	+	chr4.4:67504644-67504663	None:intergenic	65.0%
	CCGCCACCGGGACGAAGAAA+AGG	-	chr4.4:67503904-67503923	MS.gene77682:CDS	65.0%
	GCTTGAGGCTGCGGCTGGTA+TGG	-	chr4.4:67504496-67504515	MS.gene77682:CDS	65.0%
	GGCTGATGCGCTTGAGGCTG+CGG	-	chr4.4:67504487-67504506	MS.gene77682:CDS	65.0%
	TAAGTGGGCACAGCCGACGC+AGG	+	chr4.4:67505238-67505257	None:intergenic	65.0%
	TCACCAGAAGCAGGGGGTGG+GGG	+	chr4.4:67505511-67505530	None:intergenic	65.0%
	TGAGGCTGCGGCTGGTATGG+TGG	-	chr4.4:67504499-67504518	MS.gene77682:CDS	65.0%
!	GGTGGGCCTGGAGGTACAAC+AGG	+	chr4.4:67505217-67505236	None:intergenic	65.0%
!!	CCCAGGAGGAGCACTCCCAA+TGG	+	chr4.4:67505320-67505339	None:intergenic	65.0%
!!	CCCATTGGGAGTGCTCCTCC+TGG	-	chr4.4:67505316-67505335	MS.gene77682:CDS	65.0%
!!	GGAGGCACTCCTGTCCCATG+GGG	-	chr4.4:67505346-67505365	MS.gene77682:CDS	65.0%
	ACCAGGGGGACCTGTCGGCA+TGG	+	chr4.4:67505482-67505501	None:intergenic	70.0%
	ACCGGCGGCAACACCCGAAG+CGG	-	chr4.4:67503777-67503796	MS.gene77682:CDS	70.0%
	ACCTCCAGGCCCACCTGCGT+CGG	-	chr4.4:67505222-67505241	MS.gene77682:CDS	70.0%
	ACTCCCCCACCCCCTGCTTC+TGG	-	chr4.4:67505505-67505524	MS.gene77682:CDS	70.0%
	AGAACGGCGAAGCCGCCACC+GGG	-	chr4.4:67503892-67503911	MS.gene77682:CDS	70.0%
	AGAAGCAGGGGGTGGGGGAG+TGG	+	chr4.4:67505506-67505525	None:intergenic	70.0%
	CAGAACGGCGAAGCCGCCAC+CGG	-	chr4.4:67503891-67503910	MS.gene77682:CDS	70.0%
	CCAGGGGGACCTGTCGGCAT+GGG	+	chr4.4:67505481-67505500	None:intergenic	70.0%
	CCTTATGCTCCTCCCCCGCC+TGG	-	chr4.4:67505394-67505413	MS.gene77682:CDS	70.0%
	GAAGTAGGGGCAGGAGGGGG+AGG	+	chr4.4:67505709-67505728	None:intergenic	70.0%
	GATGCGCTTGAGGCTGCGGC+TGG	-	chr4.4:67504491-67504510	MS.gene77682:CDS	70.0%
	GGGGACCTGTCGGCATGGGT+GGG	+	chr4.4:67505477-67505496	None:intergenic	70.0%
	GTGGCACCAGGGGGACCTGT+CGG	+	chr4.4:67505487-67505506	None:intergenic	70.0%
	TGGGCACAGCCGACGCAGGT+GGG	+	chr4.4:67505234-67505253	None:intergenic	70.0%
!	CCAGGCGGGGGAGGAGCATA+AGG	+	chr4.4:67505397-67505416	None:intergenic	70.0%
!	TCCGCTTCGGGTGTTGCCGC+CGG	+	chr4.4:67503781-67503800	None:intergenic	70.0%
	ACAGCCGACGCAGGTGGGCC+TGG	+	chr4.4:67505229-67505248	None:intergenic	75.0%
	CCCATGCCGACAGGTCCCCC+TGG	-	chr4.4:67505478-67505497	MS.gene77682:CDS	75.0%
	GGGGGACCTGTCGGCATGGG+TGG	+	chr4.4:67505478-67505497	None:intergenic	75.0%
	GGGGTGGGGGAGTGGCACCA+GGG	+	chr4.4:67505498-67505517	None:intergenic	75.0%
	GGGTGGGGGAGTGGCACCAG+GGG	+	chr4.4:67505497-67505516	None:intergenic	75.0%
	GGTGGGGGAGTGGCACCAGG+GGG	+	chr4.4:67505496-67505515	None:intergenic	75.0%
	GTAGGGGCAGGAGGGGGAGG+TGG	+	chr4.4:67505706-67505725	None:intergenic	75.0%
	GTGGGCACAGCCGACGCAGG+TGG	+	chr4.4:67505235-67505254	None:intergenic	75.0%
!	GCAGGAGGGGGAGGTGGTGG+AGG	+	chr4.4:67505700-67505719	None:intergenic	75.0%
!	GCCGACGCAGGTGGGCCTGG+AGG	+	chr4.4:67505226-67505245	None:intergenic	80.0%
!	GGGGCAGGAGGGGGAGGTGG+TGG	+	chr4.4:67505703-67505722	None:intergenic	80.0%
!	GGGGGTGGGGGAGTGGCACC+AGG	+	chr4.4:67505499-67505518	None:intergenic	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	67503630	67505921	67503630	ID=MS.gene77682
chr4.4	mRNA	67503630	67505921	67503630	ID=MS.gene77682.t1;Parent=MS.gene77682
chr4.4	exon	67503630	67505921	67503630	ID=MS.gene77682.t1.exon1;Parent=MS.gene77682.t1
chr4.4	CDS	67503630	67505921	67503630	ID=cds.MS.gene77682.t1;Parent=MS.gene77682.t1

Gene Sequence

>MS.gene77682

ATGGAGGCTCTCGATTCCAATCCACTACCGCTCAACGATAAACCCGAAACCCTAATTCCCCAAAACGGCATCAACGGCACCACCACCACCGATAACACCGATTCACGTCCACCGATTCCAAATCCACTTCTCTCCGAAAACGGTTTAACCGGCGGCAACACCCGAAGCGGAACCGACAAAGATCAATCAGGCGGAGAAGAAGAAACAACAAGCAAACGACGCCGTAGAAGCCGATGGGACCCACAGCCAGATTCAAACGATCAGAACGGCGAAGCCGCCACCGGGACGAAGAAAAGGAAATCCAGATGGGCTGATGACGAACCGAAACCGGTAATTCAACTTCCTGATTTCATGGGTGGTATTGAATTTGATCCTGAAATTCAAACATTGAATAGTAGATTGCTTGAGATAAGTAGAATGTTGCAATCTGGTTTGCCTTTAGATGATCGTCCTGAAGGGGCTAGATCTCCTTCACCTGAACCTATATATGATAATATGGGGATTAGGATTAATACTAGAGAGTATCGTGCTCGTGAGAGGTTTCAGAAAGAGAGACAAGAGATTATTTCACAGATTATTAAGAAAAATCCTGCTTTTAAGCCACCTGCTGATTATAGACCACCAAAGCTTCAGAAGAAGCTTTATATACCGATGAAAGAGTATCCGGGGTATAATTTTATTGGGCTTATTATTGGGCCTAGGGGGAATACTCAGAAGAGGATGGAGAAGGAGACTGGTGCTAAGATTGTGATTCGGGGTAAAGGGTCGGTGAAAGAAGGTAGGTTGCAGCAGAAGAGGGATTTGAAACCGGATCCGTCGGAGAATGAGGATTTGCATGTTTTGGTTGAGGCGGATACGGCTGATGCGCTTGAGGCTGCGGCTGGTATGGTGGAGAAGCTATTGCAGCCTGTGGATGAGGTTTTGAATGAGCATAAGAGGCAGCAACTTAGGGAGCTTGCGGCTTTGAATGGGACGATTAGGGATGAAGAGTATTGTAGGTTGTGTGGTGAGCCTGGGCATCGGCAGTATGCGTGTCCTACAAGGACTTCGACGTTTAAGAGTGAGGTTGTTTGTAAGCATTGTGGGGATGGTGGTCATCCTAGTATTGATTGTCCGGTGAAGGGTGCTACTGGGAAGAAGATGGATGATGAGTATCAGAATTTCTTGGCTGAGTTGGGTGGTACAGTTCCTGAATCTGCGATTAAGACAACTAGTACTTTGGCAATTGGTTCGGGCAGTTCTGGAAGCAATCCTCCTTGGGCTAACAATTCTGGTTCTGGTGGTAGTACACCACAAGCTGGATTAGGGGCTGGTGCGGTTAAAAAGGAAATTGATGATACAAATTTGTACATTGGGTATTTGCCACCCACTCTTGAGGATGATGGTTTGATCCAATTGTTTCAACAATTTGGTGAGATTGTTATGGCAAAAGTTATTAAGGATCGAATGACAGGTTTGAGTAAAGGATACGGGTTTGTGAAGTATGCTGATATCACAATGGCAAACAATGCAATTGTTGCCATGAATGGCTATCGCCTTGAGGGTCGAACTATTGCAGTAAGAGTTGCTGGTAAGCCTCCTCAGCCTGTTGTACCTCCAGGCCCACCTGCGTCGGCTGTGCCCACTTATCCTGTTCAAAGCCAGCCAATTGGTGCCTATCCATCCCAACAATATACCGGTGGCGGTCCCATTGGGAGTGCTCCTCCTGGGAGTTATGGAGGCACTCCTGTCCCATGGGGACCTCCTATTCCTCCTCCATACAATCCTTATGCTCCTCCCCCGCCTGGATCAACTATGTATCCTCCTATGCATGGTCAACCTATGCCTCCTTATGGTGTTCAATACCCACCCATGCCGACAGGTCCCCCTGGTGCCACTCCCCCACCCCCTGCTTCTGGTGAAATGCAGCAGAATTACCCTCCTGGGGTGCAGTCTGATAATAATTCATCTACTCAGTCTGTACCGACCAACATGTATGGGAACACAATGCCTTCAATGCCACCAGCAGCTCCACCAACATATCCTGCATCTTATGGATATCCTCCTCCACCTTACTATAATGCAGTTCCTCCACCACCTCCCCCTCCTGCCCCTACTTCAGACCAATCACACAGCATTGCAAATGCGCCTTGGGCCAGTAATCATTCCGCCCCTCCTCCTCCTTCGTCTGCAGATCAATCCCAGAGCATTGGAAATGTGCCTCCACCTATGCCTCCTCCTAACTCATCGGCGGAAAAGACGTCATATGGAGCTGATTCTGAGTATGAGAAATTCATGGCAGAGATGAAATGA

Protein sequence

>MS.gene77682.t1

MEALDSNPLPLNDKPETLIPQNGINGTTTTDNTDSRPPIPNPLLSENGLTGGNTRSGTDKDQSGGEEETTSKRRRRSRWDPQPDSNDQNGEAATGTKKRKSRWADDEPKPVIQLPDFMGGIEFDPEIQTLNSRLLEISRMLQSGLPLDDRPEGARSPSPEPIYDNMGIRINTREYRARERFQKERQEIISQIIKKNPAFKPPADYRPPKLQKKLYIPMKEYPGYNFIGLIIGPRGNTQKRMEKETGAKIVIRGKGSVKEGRLQQKRDLKPDPSENEDLHVLVEADTADALEAAAGMVEKLLQPVDEVLNEHKRQQLRELAALNGTIRDEEYCRLCGEPGHRQYACPTRTSTFKSEVVCKHCGDGGHPSIDCPVKGATGKKMDDEYQNFLAELGGTVPESAIKTTSTLAIGSGSSGSNPPWANNSGSGGSTPQAGLGAGAVKKEIDDTNLYIGYLPPTLEDDGLIQLFQQFGEIVMAKVIKDRMTGLSKGYGFVKYADITMANNAIVAMNGYRLEGRTIAVRVAGKPPQPVVPPGPPASAVPTYPVQSQPIGAYPSQQYTGGGPIGSAPPGSYGGTPVPWGPPIPPPYNPYAPPPPGSTMYPPMHGQPMPPYGVQYPPMPTGPPGATPPPPASGEMQQNYPPGVQSDNNSSTQSVPTNMYGNTMPSMPPAAPPTYPASYGYPPPPYYNAVPPPPPPPAPTSDQSHSIANAPWASNHSAPPPPSSADQSQSIGNVPPPMPPPNSSAEKTSYGADSEYEKFMAEMK