Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77710.t1 | RHN59829.1 | 99.7 | 397 | 1 | 0 | 1 | 397 | 1 | 397 | 2.50E-216 | 761.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77710.t1 | Q8L5U0 | 84.1 | 397 | 63 | 0 | 1 | 397 | 1 | 397 | 7.4e-185 | 647.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77710.t1 | A0A396I2I5 | 99.7 | 397 | 1 | 0 | 1 | 397 | 1 | 397 | 1.8e-216 | 761.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051756 | MS.gene77710 | 0.8131 | 3.04E-51 | -1.69E-46 |
| MS.gene051903 | MS.gene77710 | 0.807831 | 4.17E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene42526 | MS.gene77710 | PPI |
| MS.gene018309 | MS.gene77710 | PPI |
| MS.gene77710 | MS.gene86150 | PPI |
| MS.gene017884 | MS.gene77710 | PPI |
| MS.gene77710 | MS.gene37293 | PPI |
| MS.gene77710 | MS.gene014112 | PPI |
| MS.gene018310 | MS.gene77710 | PPI |
| MS.gene39043 | MS.gene77710 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77710.t1 | MTR_0173s0030 | 99.496 | 397 | 2 | 0 | 1 | 397 | 1 | 397 | 0.0 | 805 |
| MS.gene77710.t1 | MTR_2g034320 | 46.591 | 88 | 28 | 4 | 97 | 183 | 20 | 89 | 2.57e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77710.t1 | AT5G42970 | 84.131 | 397 | 63 | 0 | 1 | 397 | 1 | 397 | 0.0 | 687 |
Find 71 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTATATATCAGGTGTATT+TGG | 0.162807 | 4.4:-68066426 | MS.gene77710:intron |
| TCTGCAAAAGCATCAATTTC+AGG | 0.220902 | 4.4:+68066386 | None:intergenic |
| TTTGGTTATTAGAGAGAAAT+TGG | 0.222768 | 4.4:-68069645 | MS.gene77710:CDS |
| ACTGTTCTATCTTCTGTCTT+TGG | 0.232195 | 4.4:+68071704 | None:intergenic |
| CAAATTGAGAAAAGGCAAAT+AGG | 0.242371 | 4.4:-68068873 | MS.gene77710:CDS |
| GCAAACTTTATACTAATATT+AGG | 0.261575 | 4.4:-68066198 | MS.gene77710:intron |
| CATTAATAAAGCATCATTCT+TGG | 0.283000 | 4.4:-68069104 | MS.gene77710:CDS |
| TCAAGCTCTAAATGATGTTT+TGG | 0.311421 | 4.4:-68064899 | MS.gene77710:CDS |
| TGCCGTTACGTGCACAATAT+TGG | 0.351744 | 4.4:-68066700 | MS.gene77710:CDS |
| GAGAGAAACCTTCTGCAGTA+TGG | 0.365955 | 4.4:+68066515 | None:intergenic |
| AGTTGTGTCGTTTGAAGAAC+AGG | 0.368584 | 4.4:-68070967 | MS.gene77710:intron |
| AGATCTGAAGAGGAAGTTCT+TGG | 0.369986 | 4.4:-68068925 | MS.gene77710:CDS |
| AAATTGAGAAAAGGCAAATA+GGG | 0.370512 | 4.4:-68068872 | MS.gene77710:CDS |
| TCCAGACAATTTCACTGTGC+TGG | 0.378815 | 4.4:-68066257 | MS.gene77710:CDS |
| TCTTGTAGCTTTGATGAGTT+AGG | 0.384222 | 4.4:-68066075 | MS.gene77710:intron |
| GGCAATAATCCAAGTCAAAC+AGG | 0.393367 | 4.4:+68064847 | None:intergenic |
| GCAATCTCTTTCTGTGTCTC+AGG | 0.418879 | 4.4:+68071020 | None:intergenic |
| TGCACAATATTGGCAGGTGC+AGG | 0.426537 | 4.4:-68066690 | MS.gene77710:CDS |
| GGGTAATTGATGATACATTC+AGG | 0.436412 | 4.4:-68069434 | MS.gene77710:CDS |
| TGCTGAAGAGTTGGGAAGGT+TGG | 0.443905 | 4.4:-68071045 | MS.gene77710:CDS |
| TCCAGCACAGTGAAATTGTC+TGG | 0.443989 | 4.4:+68066256 | None:intergenic |
| AATCCAAGTCAAACAGGAAC+AGG | 0.449263 | 4.4:+68064853 | None:intergenic |
| AGCATCAAGAATGATTTATG+AGG | 0.485288 | 4.4:-68065619 | MS.gene77710:CDS |
| CAGTGAAATTGTCTGGAAGA+AGG | 0.485991 | 4.4:+68066263 | None:intergenic |
| AGTATTGAATTTGAAGTACA+AGG | 0.490159 | 4.4:-68069062 | MS.gene77710:intron |
| AGTGGTATTGACCTAGATTC+AGG | 0.492697 | 4.4:-68069572 | MS.gene77710:CDS |
| GATGAGTTAGGCACACTGTT+GGG | 0.493284 | 4.4:-68066063 | MS.gene77710:CDS |
| GATTTATGAGGATAGGATGA+AGG | 0.501314 | 4.4:-68065607 | MS.gene77710:CDS |
| AAATTATGTACCTTGGGAGG+TGG | 0.506288 | 4.4:+68066036 | None:intergenic |
| GAAATTAATGAGGAAGCGCT+TGG | 0.507602 | 4.4:-68066738 | MS.gene77710:CDS |
| ACACTGAAGAGTTGCAAAGA+TGG | 0.510433 | 4.4:-68065433 | MS.gene77710:CDS |
| ACATATCTCAAATTGAGAAA+AGG | 0.510694 | 4.4:-68068881 | MS.gene77710:CDS |
| GATGAAGGGATCTATTGATC+AGG | 0.510987 | 4.4:-68065592 | MS.gene77710:intron |
| AGAAGAATTGAAACCACATC+AGG | 0.513961 | 4.4:-68066366 | MS.gene77710:intron |
| TGTTCTTGCCACACTGTACA+AGG | 0.519005 | 4.4:-68066652 | MS.gene77710:intron |
| GAGACCTCATTCCTGAATCT+AGG | 0.525371 | 4.4:+68069561 | None:intergenic |
| AGTGAAATTGTCTGGAAGAA+GGG | 0.526038 | 4.4:+68066264 | None:intergenic |
| AGAGAAACCTTCTGCAGTAT+GGG | 0.529187 | 4.4:+68066516 | None:intergenic |
| TTGGATAGCATGGGCAAGAA+GGG | 0.533962 | 4.4:-68064880 | MS.gene77710:CDS |
| CAAACTTTAATAACCTGATG+TGG | 0.534249 | 4.4:+68066353 | None:intergenic |
| AAATTAATGAGGAAGCGCTT+GGG | 0.534615 | 4.4:-68066737 | MS.gene77710:CDS |
| CACTGAAGAGTTGCAAAGAT+GGG | 0.536188 | 4.4:-68065432 | MS.gene77710:CDS |
| AATTGCTTGTTTATACCTTG+AGG | 0.536370 | 4.4:-68069394 | MS.gene77710:intron |
| TCAAATTCAATACTTCATGC+TGG | 0.537152 | 4.4:+68069071 | None:intergenic |
| GTTGCAAAGATGGGACCAGC+AGG | 0.537433 | 4.4:-68065423 | MS.gene77710:intron |
| TTTGGATAGCATGGGCAAGA+AGG | 0.544420 | 4.4:-68064881 | MS.gene77710:CDS |
| TGATGAGTTAGGCACACTGT+TGG | 0.554252 | 4.4:-68066064 | MS.gene77710:CDS |
| GTTACGTGCACAATATTGGC+AGG | 0.556349 | 4.4:-68066696 | MS.gene77710:CDS |
| CAAATGACATTCTTCAAGCT+AGG | 0.557041 | 4.4:-68071651 | MS.gene77710:CDS |
| GGCTGAGCTTTATGAATCTG+AGG | 0.561921 | 4.4:-68069624 | MS.gene77710:CDS |
| AGCTGACGCGACACTACTAA+AGG | 0.562961 | 4.4:+68071080 | None:intergenic |
| CAAGAATGATTTATGAGGAT+AGG | 0.564825 | 4.4:-68065614 | MS.gene77710:CDS |
| GATCTATCCCATACTGCAGA+AGG | 0.565134 | 4.4:-68066523 | MS.gene77710:intron |
| ATATTACAGGGAAATTAATG+AGG | 0.565776 | 4.4:-68066748 | MS.gene77710:intron |
| AATTATGTACCTTGGGAGGT+GGG | 0.571748 | 4.4:+68066037 | None:intergenic |
| ATTTATGAGGATAGGATGAA+GGG | 0.574286 | 4.4:-68065606 | MS.gene77710:CDS |
| GCACAATATTGGCAGGTGCA+GGG | 0.576796 | 4.4:-68066689 | MS.gene77710:CDS |
| CTAGGATCTTAGATCTGAAG+AGG | 0.577929 | 4.4:-68068935 | MS.gene77710:CDS |
| TTTATGAATCTGAGGAGCAA+TGG | 0.600846 | 4.4:-68069616 | MS.gene77710:CDS |
| TGCCAATATTGTGCACGTAA+CGG | 0.607348 | 4.4:+68066698 | None:intergenic |
| TTGACCTAGATTCAGGAATG+AGG | 0.609769 | 4.4:-68069565 | MS.gene77710:intron |
| GTTGGGAATCCCACCTCCCA+AGG | 0.613288 | 4.4:-68066046 | MS.gene77710:intron |
| GATTATGCTCAATCATAGCT+CGG | 0.614554 | 4.4:+68066233 | None:intergenic |
| AATTGAGAAAAGGCAAATAG+GGG | 0.620222 | 4.4:-68068871 | MS.gene77710:CDS |
| AGATATGTCATAATATCGCA+AGG | 0.635346 | 4.4:+68068896 | None:intergenic |
| AATCTTTACCTTGTACAGTG+TGG | 0.647262 | 4.4:+68066644 | None:intergenic |
| ATGAAATTATGTACCTTGGG+AGG | 0.648793 | 4.4:+68066033 | None:intergenic |
| TCTTCAAACGACACAACTCG+AGG | 0.694248 | 4.4:+68070972 | None:intergenic |
| GCAAGAACACGAGAACGTTG+AGG | 0.695486 | 4.4:+68066666 | None:intergenic |
| AAAGCTGCTCAGATGCTCAG+TGG | 0.697893 | 4.4:-68069590 | MS.gene77710:CDS |
| TGATGTGAAACAGTGATGGA+GGG | 0.716084 | 4.4:-68071757 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 68064861 | 68071764 | 68064861 | ID=MS.gene77710 |
| chr4.4 | mRNA | 68064861 | 68071764 | 68064861 | ID=MS.gene77710.t1;Parent=MS.gene77710 |
| chr4.4 | exon | 68071635 | 68071764 | 68071635 | ID=MS.gene77710.t1.exon1;Parent=MS.gene77710.t1 |
| chr4.4 | CDS | 68071635 | 68071764 | 68071635 | ID=cds.MS.gene77710.t1;Parent=MS.gene77710.t1 |
| chr4.4 | exon | 68070968 | 68071119 | 68070968 | ID=MS.gene77710.t1.exon2;Parent=MS.gene77710.t1 |
| chr4.4 | CDS | 68070968 | 68071119 | 68070968 | ID=cds.MS.gene77710.t1;Parent=MS.gene77710.t1 |
| chr4.4 | exon | 68069566 | 68069669 | 68069566 | ID=MS.gene77710.t1.exon3;Parent=MS.gene77710.t1 |
| chr4.4 | CDS | 68069566 | 68069669 | 68069566 | ID=cds.MS.gene77710.t1;Parent=MS.gene77710.t1 |
| chr4.4 | exon | 68069395 | 68069455 | 68069395 | ID=MS.gene77710.t1.exon4;Parent=MS.gene77710.t1 |
| chr4.4 | CDS | 68069395 | 68069455 | 68069395 | ID=cds.MS.gene77710.t1;Parent=MS.gene77710.t1 |
| chr4.4 | exon | 68069063 | 68069155 | 68069063 | ID=MS.gene77710.t1.exon5;Parent=MS.gene77710.t1 |
| chr4.4 | CDS | 68069063 | 68069155 | 68069063 | ID=cds.MS.gene77710.t1;Parent=MS.gene77710.t1 |
| chr4.4 | exon | 68068867 | 68068967 | 68068867 | ID=MS.gene77710.t1.exon6;Parent=MS.gene77710.t1 |
| chr4.4 | CDS | 68068867 | 68068967 | 68068867 | ID=cds.MS.gene77710.t1;Parent=MS.gene77710.t1 |
| chr4.4 | exon | 68066653 | 68066761 | 68066653 | ID=MS.gene77710.t1.exon7;Parent=MS.gene77710.t1 |
| chr4.4 | CDS | 68066653 | 68066761 | 68066653 | ID=cds.MS.gene77710.t1;Parent=MS.gene77710.t1 |
| chr4.4 | exon | 68066524 | 68066568 | 68066524 | ID=MS.gene77710.t1.exon8;Parent=MS.gene77710.t1 |
| chr4.4 | CDS | 68066524 | 68066568 | 68066524 | ID=cds.MS.gene77710.t1;Parent=MS.gene77710.t1 |
| chr4.4 | exon | 68066367 | 68066435 | 68066367 | ID=MS.gene77710.t1.exon9;Parent=MS.gene77710.t1 |
| chr4.4 | CDS | 68066367 | 68066435 | 68066367 | ID=cds.MS.gene77710.t1;Parent=MS.gene77710.t1 |
| chr4.4 | exon | 68066199 | 68066290 | 68066199 | ID=MS.gene77710.t1.exon10;Parent=MS.gene77710.t1 |
| chr4.4 | CDS | 68066199 | 68066290 | 68066199 | ID=cds.MS.gene77710.t1;Parent=MS.gene77710.t1 |
| chr4.4 | exon | 68066047 | 68066089 | 68066047 | ID=MS.gene77710.t1.exon11;Parent=MS.gene77710.t1 |
| chr4.4 | CDS | 68066047 | 68066089 | 68066047 | ID=cds.MS.gene77710.t1;Parent=MS.gene77710.t1 |
| chr4.4 | exon | 68065593 | 68065652 | 68065593 | ID=MS.gene77710.t1.exon12;Parent=MS.gene77710.t1 |
| chr4.4 | CDS | 68065593 | 68065652 | 68065593 | ID=cds.MS.gene77710.t1;Parent=MS.gene77710.t1 |
| chr4.4 | exon | 68065424 | 68065483 | 68065424 | ID=MS.gene77710.t1.exon13;Parent=MS.gene77710.t1 |
| chr4.4 | CDS | 68065424 | 68065483 | 68065424 | ID=cds.MS.gene77710.t1;Parent=MS.gene77710.t1 |
| chr4.4 | exon | 68064861 | 68064935 | 68064861 | ID=MS.gene77710.t1.exon14;Parent=MS.gene77710.t1 |
| chr4.4 | CDS | 68064861 | 68064935 | 68064861 | ID=cds.MS.gene77710.t1;Parent=MS.gene77710.t1 |
| Gene Sequence |
| Protein sequence |
