AlfalfaGEDB | MS.gene78208

M. sativa cultivar XinJiangDaYe / MS.gene78208

KEGG: K07297
GO: GO:0005575 GO:0016021 GO:0031224 GO:0110165
PFAM: PF03006.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78208.t1	KEH16922.1	83.9	87	13	1	1	87	1	86	4.20E-31	143.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78208.t1	B7F9G7	69.1	55	17	0	12	66	49	103	1.2e-18	93.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78208.t1	A0A072THN2	83.9	87	13	1	1	87	1	86	3.0e-31	143.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene78208	0.804123	2.51E-49	-1.69E-46
MS.gene050807	MS.gene78208	0.808022	3.80E-50	-1.69E-46
MS.gene050987	MS.gene78208	0.80932	2.01E-50	-1.69E-46
MS.gene051243	MS.gene78208	0.819005	1.46E-52	-1.69E-46
MS.gene053188	MS.gene78208	0.803912	2.78E-49	-1.69E-46
MS.gene05364	MS.gene78208	0.80304	4.21E-49	-1.69E-46
MS.gene053931	MS.gene78208	0.808634	2.81E-50	-1.69E-46
MS.gene054657	MS.gene78208	0.811991	5.31E-51	-1.69E-46
MS.gene056760	MS.gene78208	0.806486	8.04E-50	-1.69E-46
MS.gene057060	MS.gene78208	0.833745	4.47E-56	-1.69E-46
MS.gene057208	MS.gene78208	0.805846	1.10E-49	-1.69E-46
MS.gene057392	MS.gene78208	0.838483	2.80E-57	-1.69E-46
MS.gene057869	MS.gene78208	0.803192	3.92E-49	-1.69E-46
MS.gene058634	MS.gene78208	0.801666	8.09E-49	-1.69E-46
MS.gene05978	MS.gene78208	0.825817	3.81E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78208.t1	MTR_0066s0040	97.701	87	1	1	1	87	1	86	7.19e-58	173
MS.gene78208.t1	MTR_4g115580	56.061	66	29	0	4	69	38	103	6.59e-23	90.1
MS.gene78208.t1	MTR_5g015270	54.412	68	31	0	7	74	28	95	1.73e-20	83.6
MS.gene78208.t1	MTR_5g015270	54.412	68	31	0	7	74	28	95	2.08e-20	83.6
MS.gene78208.t1	MTR_3g103240	60.714	56	22	0	7	62	32	87	4.79e-20	82.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78208.t1	AT4G30850	52.381	84	34	1	1	78	19	102	1.74e-25	97.1
MS.gene78208.t1	AT4G30850	52.381	84	34	1	1	78	19	102	1.74e-25	97.1
MS.gene78208.t1	AT2G24150	67.241	58	19	0	12	69	33	90	2.08e-24	94.0
MS.gene78208.t1	AT5G20270	56.579	76	24	2	3	69	37	112	2.70e-24	93.6
MS.gene78208.t1	AT4G38320	51.282	78	33	1	2	74	23	100	1.46e-20	84.0
MS.gene78208.t1	AT4G37680	51.282	78	33	1	2	74	23	100	2.07e-20	83.6
MS.gene78208.t1	AT4G37680	51.282	78	33	1	2	74	23	100	2.07e-20	83.6
MS.gene78208.t1	AT4G37680	51.282	78	33	1	2	74	23	100	2.07e-20	83.6
MS.gene78208.t1	AT4G37680	51.282	78	33	1	2	74	28	105	2.13e-20	83.6
MS.gene78208.t1	AT4G37680	51.282	78	33	1	2	74	28	105	2.13e-20	83.6

Find 24 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTGTTCTTGTACAGTCATT+TGG	0.300362	2.2:+32748391	MS.gene78208:intron
TTGTTCTTGTACAGTCATTT+GGG	0.334474	2.2:+32748392	MS.gene78208:intron
AAAAGACAACGAGTTCATAT+TGG	0.344022	2.2:+32748219	MS.gene78208:CDS
GCGATGGCGGCGACGGAAGC+TGG	0.345791	2.2:+32748434	MS.gene78208:CDS
AGTTCATATTGGATTATTAC+CGG	0.383152	2.2:+32748230	MS.gene78208:CDS
GCGACGGAAGCTGGAGGAGG+AGG	0.408012	2.2:+32748443	MS.gene78208:CDS
ACAACGAAACGCTAAATGTT+TGG	0.429326	2.2:+32748299	MS.gene78208:CDS
GGTGAAATACGAGGAATTGC+CGG	0.439654	2.2:+32748189	MS.gene78208:CDS
ACGGAAGCTGGAGGAGGAGG+AGG	0.442291	2.2:+32748446	MS.gene78208:CDS
CGAATATGAAGGGTAATTAT+CGG	0.467352	2.2:+32748146	None:intergenic
CCGGTCGGAATGGTCGGTTA+AGG	0.472175	2.2:+32748249	MS.gene78208:CDS
GCGGCGACGGAAGCTGGAGG+AGG	0.486075	2.2:+32748440	MS.gene78208:CDS
TTTAATATTTGCAGCGATGG+CGG	0.490595	2.2:+32748421	MS.gene78208:CDS
TGGATTATTACCGGTCGGAA+TGG	0.492532	2.2:+32748239	MS.gene78208:CDS
GAAGCTGGAGGAGGAGGAGG+TGG	0.496794	2.2:+32748449	MS.gene78208:CDS
TTATTACCGGTCGGAATGGT+CGG	0.518312	2.2:+32748243	MS.gene78208:CDS
ATTTGCAGCGATGGCGGCGA+CGG	0.523006	2.2:+32748427	MS.gene78208:CDS
GCGGAAGAGAACGTTGCGAT+TGG	0.550524	2.2:+32748168	MS.gene78208:CDS
TTCTTGTACAGTCATTTGGG+AGG	0.573521	2.2:+32748395	MS.gene78208:intron
CATATTGGATTATTACCGGT+CGG	0.594477	2.2:+32748234	MS.gene78208:CDS
ATGGCGGCGACGGAAGCTGG+AGG	0.636720	2.2:+32748437	MS.gene78208:CDS
CCTTAACCGACCATTCCGAC+CGG	0.662396	2.2:-32748249	None:intergenic
ATATGAAGGGTAATTATCGG+CGG	0.702822	2.2:+32748149	None:intergenic
GTTGCGATTGGTGAAATACG+AGG	0.720016	2.2:+32748180	MS.gene78208:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGTTCATATTGGATTATTAC+CGG	+	chr2.2:32748230-32748249	MS.gene78208:CDS	25.0%
	AAAAGACAACGAGTTCATAT+TGG	+	chr2.2:32748219-32748238	MS.gene78208:CDS	30.0%
	TTGTTCTTGTACAGTCATTT+GGG	+	chr2.2:32748392-32748411	MS.gene78208:intron	30.0%
!	CTTTTTAATATTTGCAGCGA+TGG	+	chr2.2:32748418-32748437	MS.gene78208:CDS	30.0%
!	GTTTTCTGTCTAATATCTAC+AGG	+	chr2.2:32748471-32748490	MS.gene78208:CDS	30.0%
!	TCGTTGTCTTTTAGATATTC+CGG	-	chr2.2:32748211-32748230	None:intergenic	30.0%
	AACAACAACGATGTCAAACA+TGG	-	chr2.2:32748374-32748393	None:intergenic	35.0%
	ACAACGAAACGCTAAATGTT+TGG	+	chr2.2:32748299-32748318	MS.gene78208:CDS	35.0%
	CATATTGGATTATTACCGGT+CGG	+	chr2.2:32748234-32748253	MS.gene78208:CDS	35.0%
	GTTGTTCTTGTACAGTCATT+TGG	+	chr2.2:32748391-32748410	MS.gene78208:intron	35.0%
	TTTAATATTTGCAGCGATGG+CGG	+	chr2.2:32748421-32748440	MS.gene78208:CDS	35.0%
	TTCTTGTACAGTCATTTGGG+AGG	+	chr2.2:32748395-32748414	MS.gene78208:intron	40.0%
	GGTGAAATACGAGGAATTGC+CGG	+	chr2.2:32748189-32748208	MS.gene78208:CDS	45.0%
	GTTGCGATTGGTGAAATACG+AGG	+	chr2.2:32748180-32748199	MS.gene78208:CDS	45.0%
	TGGATTATTACCGGTCGGAA+TGG	+	chr2.2:32748239-32748258	MS.gene78208:CDS	45.0%
	TTATTACCGGTCGGAATGGT+CGG	+	chr2.2:32748243-32748262	MS.gene78208:CDS	45.0%
!	CTTTGTTCAGCGTTTTCGCG+TGG	+	chr2.2:32748275-32748294	MS.gene78208:CDS	50.0%
	CCTTAACCGACCATTCCGAC+CGG	-	chr2.2:32748252-32748271	None:intergenic	55.0%
	GCGGAAGAGAACGTTGCGAT+TGG	+	chr2.2:32748168-32748187	MS.gene78208:CDS	55.0%
	CCGGTCGGAATGGTCGGTTA+AGG	+	chr2.2:32748249-32748268	MS.gene78208:CDS	60.0%
!	ATTTGCAGCGATGGCGGCGA+CGG	+	chr2.2:32748427-32748446	MS.gene78208:CDS	60.0%
	ACGGAAGCTGGAGGAGGAGG+AGG	+	chr2.2:32748446-32748465	MS.gene78208:CDS	65.0%
	GAAGCTGGAGGAGGAGGAGG+TGG	+	chr2.2:32748449-32748468	MS.gene78208:CDS	65.0%
	ATGGCGGCGACGGAAGCTGG+AGG	+	chr2.2:32748437-32748456	MS.gene78208:CDS	70.0%
	GCGACGGAAGCTGGAGGAGG+AGG	+	chr2.2:32748443-32748462	MS.gene78208:CDS	70.0%
	GCGGCGACGGAAGCTGGAGG+AGG	+	chr2.2:32748440-32748459	MS.gene78208:CDS	75.0%
!	GCGATGGCGGCGACGGAAGC+TGG	+	chr2.2:32748434-32748453	MS.gene78208:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	32748151	32748499	32748151	ID=MS.gene78208
chr2.2	mRNA	32748151	32748499	32748151	ID=MS.gene78208.t1;Parent=MS.gene78208
chr2.2	exon	32748151	32748323	32748151	ID=MS.gene78208.t1.exon1;Parent=MS.gene78208.t1
chr2.2	CDS	32748151	32748323	32748151	ID=cds.MS.gene78208.t1;Parent=MS.gene78208.t1
chr2.2	exon	32748406	32748499	32748406	ID=MS.gene78208.t1.exon2;Parent=MS.gene78208.t1
chr2.2	CDS	32748406	32748499	32748406	ID=cds.MS.gene78208.t1;Parent=MS.gene78208.t1

Gene Sequence	>MS.gene78208 ATGAAGGGTAATTATCGGCGGAAGAGAACGTTGCGATTGGTGAAATACGAGGAATTGCCGGAATATCTAAAAGACAACGAGTTCATATTGGATTATTACCGGTCGGAATGGTCGGTTAAGGATGCTTTGTTCAGCGTTTTCGCGTGGCACAACGAAACGCTAAATGTTTGGACGTAAGTGTTTTTCTGTTAAGATTTTAGCAATAACTGTATTGTTGTTTCCATGTTTGACATCGTTGTTGTTGTTCTTGTACAGTCATTTGGGAGGCTTTTTAATATTTGCAGCGATGGCGGCGACGGAAGCTGGAGGAGGAGGAGGTGGTTTTCTGTCTAATATCTACAGGTATTGA
Protein sequence	>MS.gene78208.t1 MKGNYRRKRTLRLVKYEELPEYLKDNEFILDYYRSEWSVKDALFSVFAWHNETLNVWTHLGGFLIFAAMAATEAGGGGGGFLSNIYRY