AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene78663

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78663.t1 MTR_8g042910 97.822 505 10 1 1 504 109 613 0.0 1036
MS.gene78663.t1 MTR_3g010770 77.075 506 113 2 1 503 70 575 0.0 816
MS.gene78663.t1 MTR_7g108875 50.586 512 227 8 1 503 70 564 5.84e-175 504
MS.gene78663.t1 MTR_1g054880 50.395 506 236 5 1 503 65 558 1.08e-170 493
MS.gene78663.t1 MTR_7g108890 50.588 510 230 7 1 503 68 562 1.23e-170 493
MS.gene78663.t1 MTR_7g108900 51.373 510 226 10 1 503 66 560 1.42e-170 493
MS.gene78663.t1 MTR_1g088020 49.213 508 241 7 1 503 60 555 8.77e-166 481
MS.gene78663.t1 MTR_1g088225 48.819 508 241 8 1 503 60 553 3.19e-161 469
MS.gene78663.t1 MTR_1g083520 46.535 505 230 5 5 504 60 529 4.93e-156 455
MS.gene78663.t1 MTR_7g108905 48.283 466 214 7 35 499 113 552 5.79e-149 438
MS.gene78663.t1 MTR_4g124520 47.609 460 201 6 46 503 100 521 5.37e-140 414
MS.gene78663.t1 MTR_2g044810 42.744 503 242 6 1 503 78 534 1.04e-136 405
MS.gene78663.t1 MTR_2g044880 46.122 477 206 11 35 503 92 525 2.44e-135 402
MS.gene78663.t1 MTR_1g008140 45.062 486 227 8 34 503 146 607 3.67e-134 402
MS.gene78663.t1 MTR_4g025690 47.667 493 196 13 19 503 75 513 1.09e-131 392
MS.gene78663.t1 MTR_0458s0030 65.152 264 92 0 240 503 51 314 3.16e-130 381
MS.gene78663.t1 MTR_1g088040 65.152 264 92 0 240 503 51 314 3.16e-130 381
MS.gene78663.t1 MTR_8g104655 44.989 469 215 8 48 503 103 541 8.79e-130 388
MS.gene78663.t1 MTR_3g091380 45.135 483 224 9 34 503 143 597 2.41e-129 389
MS.gene78663.t1 MTR_4g087980 43.058 497 236 10 9 503 65 516 3.71e-129 386
MS.gene78663.t1 MTR_4g025730 47.046 474 193 12 32 503 92 509 3.88e-128 383
MS.gene78663.t1 MTR_7g112580 43.333 480 238 8 27 505 98 544 5.49e-127 381
MS.gene78663.t1 MTR_8g104645 51.964 331 156 2 174 503 44 372 5.19e-126 372
MS.gene78663.t1 MTR_8g042900 44.350 469 227 8 35 503 147 581 3.25e-125 378
MS.gene78663.t1 MTR_4g087970 41.036 502 241 9 9 503 64 517 2.46e-123 371
MS.gene78663.t1 MTR_4g025670 43.125 480 227 9 25 503 55 489 4.61e-122 367
MS.gene78663.t1 MTR_7g050870 43.750 480 219 9 32 503 118 554 1.49e-119 362
MS.gene78663.t1 MTR_8g023310 43.154 482 249 8 27 503 128 589 8.36e-119 362
MS.gene78663.t1 MTR_3g010780 41.970 467 233 9 35 499 140 570 2.81e-118 360
MS.gene78663.t1 MTR_4g035980 43.460 474 218 12 38 503 108 539 4.63e-118 358
MS.gene78663.t1 MTR_3g008720 51.351 333 155 4 173 503 200 527 2.33e-117 356
MS.gene78663.t1 MTR_7g053360 51.652 333 154 4 173 503 200 527 2.35e-117 356
MS.gene78663.t1 MTR_3g033690 51.351 333 155 4 173 503 199 526 6.64e-117 355
MS.gene78663.t1 MTR_3g008640 51.351 333 155 4 173 503 200 527 6.70e-117 355
MS.gene78663.t1 MTR_7g050950 40.956 481 224 8 35 503 121 553 3.54e-116 353
MS.gene78663.t1 MTR_3g008700 51.051 333 156 4 173 503 200 527 1.70e-115 351
MS.gene78663.t1 MTR_7g050980 42.708 480 224 10 32 503 119 555 2.20e-115 352
MS.gene78663.t1 MTR_2g044810 60.714 252 99 0 252 503 214 465 1.71e-113 343
MS.gene78663.t1 MTR_7g112590 37.897 504 267 11 19 502 105 582 5.53e-112 344
MS.gene78663.t1 MTR_1g083510 38.703 478 268 8 35 502 120 582 3.28e-111 342
MS.gene78663.t1 MTR_4g124550 39.220 487 247 7 32 504 143 594 5.30e-110 339
MS.gene78663.t1 MTR_1g086190 38.155 477 274 7 29 503 106 563 9.31e-105 325
MS.gene78663.t1 MTR_2g044660 50.794 315 148 3 191 505 327 634 1.07e-102 321
MS.gene78663.t1 MTR_1g086210 38.020 505 236 12 35 504 108 570 7.10e-102 317
MS.gene78663.t1 MTR_0458s0010 37.870 507 263 10 1 503 60 518 5.35e-100 311
MS.gene78663.t1 MTR_1g083490 34.449 508 277 7 3 499 109 571 9.67e-100 312
MS.gene78663.t1 MTR_1g083470 36.461 469 268 9 35 499 124 566 1.34e-99 311
MS.gene78663.t1 MTR_1g083500 36.461 469 268 9 35 499 124 566 1.34e-99 311
MS.gene78663.t1 MTR_4g005170 36.059 477 259 8 35 499 129 571 4.64e-99 312
MS.gene78663.t1 MTR_0618s0020 59.912 227 90 1 278 503 2 228 5.38e-99 298
MS.gene78663.t1 MTR_8g104580 37.232 513 238 12 1 503 62 500 7.89e-98 305
MS.gene78663.t1 MTR_4g005180 35.446 505 276 10 3 499 91 553 7.69e-97 304
MS.gene78663.t1 MTR_8g023150 40.940 447 203 11 72 499 174 578 2.98e-96 303
MS.gene78663.t1 MTR_7g110560 36.842 475 263 8 35 503 106 549 3.91e-95 299
MS.gene78663.t1 MTR_8g093460 49.169 301 141 5 201 492 243 540 2.15e-93 295
MS.gene78663.t1 MTR_3g095920 46.732 306 150 5 197 492 251 553 2.80e-90 287
MS.gene78663.t1 MTR_4g120290 32.510 526 293 10 1 502 84 571 5.51e-88 281
MS.gene78663.t1 MTR_2g436040 32.824 524 288 11 3 500 58 543 2.44e-87 281
MS.gene78663.t1 MTR_5g083620 40.845 355 196 6 157 506 14 359 8.32e-86 269
MS.gene78663.t1 MTR_7g110440 34.927 481 281 10 35 504 108 567 1.24e-83 270
MS.gene78663.t1 MTR_4g120280 32.115 520 290 10 9 502 89 571 2.13e-83 270
MS.gene78663.t1 MTR_7g110490 36.437 494 252 10 35 498 108 569 2.52e-82 266
MS.gene78663.t1 MTR_7g110510 36.437 494 252 10 35 498 108 569 2.52e-82 266
MS.gene78663.t1 MTR_2g436050 33.133 498 289 12 19 502 96 563 1.70e-79 259
MS.gene78663.t1 MTR_8g088610 41.667 324 181 5 182 500 53 373 2.91e-79 253
MS.gene78663.t1 MTR_7g107180 43.671 316 162 6 190 503 6 307 3.34e-76 242
MS.gene78663.t1 MTR_2g105560 41.587 315 166 6 191 503 41 339 3.31e-74 238
MS.gene78663.t1 MTR_7g110420 33.537 492 286 14 35 506 108 578 1.38e-71 238
MS.gene78663.t1 MTR_7g110430 30.707 495 249 11 35 504 108 533 4.01e-64 218
MS.gene78663.t1 MTR_7g111560 31.062 499 282 12 19 503 106 556 8.42e-64 217
MS.gene78663.t1 MTR_1g086540 34.770 348 194 10 156 492 19 344 4.31e-53 183
MS.gene78663.t1 MTR_8g102380 32.216 388 219 12 121 492 18 377 8.14e-52 181
MS.gene78663.t1 MTR_6g088810 37.705 305 160 8 200 492 91 377 5.25e-51 178
MS.gene78663.t1 MTR_6g012800 36.237 287 157 5 191 466 6 277 1.19e-49 173
MS.gene78663.t1 MTR_6g024165 34.451 328 179 10 177 492 31 334 2.74e-48 170
MS.gene78663.t1 MTR_7g092780 34.921 315 179 7 189 492 88 387 2.81e-48 171
MS.gene78663.t1 MTR_2g039530 36.934 287 154 8 191 470 52 318 1.67e-47 168
MS.gene78663.t1 MTR_7g102600 31.215 362 222 7 143 492 47 393 5.07e-47 168
MS.gene78663.t1 MTR_6g042660 33.557 298 169 8 175 466 22 296 6.80e-45 160
MS.gene78663.t1 MTR_5g083590 32.372 312 158 5 193 503 9 268 7.91e-44 156
MS.gene78663.t1 MTR_7g011530 33.451 284 165 4 193 466 8 277 2.11e-43 156
MS.gene78663.t1 MTR_8g102380 32.036 334 185 11 121 438 18 325 2.16e-43 157
MS.gene78663.t1 MTR_5g095490 33.766 308 175 7 197 492 67 357 1.45e-40 150
MS.gene78663.t1 MTR_1g033050 32.234 273 162 5 201 466 28 284 3.20e-39 145
MS.gene78663.t1 MTR_2g033970 28.990 307 195 6 193 493 58 347 8.71e-38 142
MS.gene78663.t1 MTR_5g012600 30.154 325 198 7 184 496 56 363 3.68e-37 140
MS.gene78663.t1 MTR_5g093970 33.582 268 157 6 206 467 54 306 5.12e-36 137
MS.gene78663.t1 MTR_6g042690 30.986 284 169 6 191 466 39 303 1.38e-35 135
MS.gene78663.t1 MTR_4g124740 28.205 312 188 9 191 492 54 339 1.33e-34 133
MS.gene78663.t1 MTR_4g051810 31.095 283 175 4 184 466 30 292 2.87e-32 126
MS.gene78663.t1 MTR_2g436600 28.065 310 185 10 193 493 57 337 3.27e-32 126
MS.gene78663.t1 MTR_8g036225 31.228 285 168 7 191 466 33 298 3.81e-32 126
MS.gene78663.t1 MTR_5g088300 30.686 277 170 5 191 466 37 292 3.84e-32 125
MS.gene78663.t1 MTR_5g088290 31.179 263 162 5 206 466 59 304 2.52e-30 121
MS.gene78663.t1 MTR_5g088280 30.496 282 174 6 187 467 35 295 3.63e-29 117
MS.gene78663.t1 MTR_2g073390 25.806 310 207 7 189 492 69 361 3.77e-24 104
MS.gene78663.t1 MTR_4g074290 25.733 307 203 8 193 492 73 361 1.91e-23 102
MS.gene78663.t1 MTR_0009s0350 25.490 306 205 7 193 492 73 361 1.53e-22 99.8
MS.gene78663.t1 MTR_0009s0340 25.490 306 205 7 193 492 73 361 1.53e-22 99.8
MS.gene78663.t1 MTR_6g025590 32.161 199 114 8 166 354 30 217 1.08e-20 91.7
MS.gene78663.t1 MTR_0408s0030 37.113 97 52 1 370 466 36 123 4.45e-17 79.0
MS.gene78663.t1 MTR_4g130790 30.114 176 111 4 208 375 39 210 1.57e-16 80.1
MS.gene78663.t1 MTR_6g024200 30.814 172 98 5 322 492 125 276 8.88e-16 78.2
MS.gene78663.t1 MTR_7g102580 43.023 86 39 2 268 348 73 153 8.04e-14 69.3
MS.gene78663.t1 MTR_7g102570 29.508 122 74 3 353 472 3 114 2.49e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78663.t1 AT5G04970 60.630 508 189 6 1 503 119 620 0.0 632
MS.gene78663.t1 AT3G10720 60.236 508 191 6 1 503 114 615 0.0 629
MS.gene78663.t1 AT4G02330 49.027 514 245 6 1 503 64 571 2.08e-174 503
MS.gene78663.t1 AT1G02810 49.336 527 234 9 1 503 60 577 5.91e-171 494
MS.gene78663.t1 AT2G47550 49.474 475 227 5 31 503 95 558 1.76e-155 454
MS.gene78663.t1 AT3G10720 74.903 259 65 0 245 503 1 259 3.61e-151 432
MS.gene78663.t1 AT4G33220 45.912 477 231 8 35 504 68 524 3.72e-136 404
MS.gene78663.t1 AT3G43270 45.957 470 229 6 35 503 80 525 2.28e-134 399
MS.gene78663.t1 AT4G33220 49.161 417 193 7 90 504 4 403 2.24e-131 387
MS.gene78663.t1 AT3G60730 46.154 442 211 6 72 503 95 519 3.82e-131 391
MS.gene78663.t1 AT2G26440 43.168 505 254 9 1 503 74 547 2.87e-129 387
MS.gene78663.t1 AT3G05620 43.802 484 227 7 27 503 96 541 3.86e-128 384
MS.gene78663.t1 AT2G45220 58.675 317 121 5 188 503 204 511 4.84e-126 377
MS.gene78663.t1 AT5G53370 42.715 501 245 12 15 503 115 585 1.55e-122 371
MS.gene78663.t1 AT3G49220 44.979 478 222 10 34 503 152 596 2.73e-122 371
MS.gene78663.t1 AT5G53370 42.715 501 245 12 15 503 151 621 5.54e-122 371
MS.gene78663.t1 AT3G49220 44.979 478 222 10 34 503 197 641 1.02e-121 371
MS.gene78663.t1 AT1G23200 42.383 512 256 11 1 503 73 554 4.65e-121 366
MS.gene78663.t1 AT1G11580 42.072 473 235 6 34 503 121 557 6.44e-121 366
MS.gene78663.t1 AT1G11580 42.072 473 235 6 34 503 142 578 1.25e-120 366
MS.gene78663.t1 AT4G02320 40.698 516 251 9 1 503 45 518 1.26e-120 364
MS.gene78663.t1 AT2G43050 44.880 459 208 9 48 503 100 516 2.55e-118 358
MS.gene78663.t1 AT1G53830 43.633 479 254 7 28 503 122 587 8.46e-118 359
MS.gene78663.t1 AT5G04960 42.137 496 236 12 10 499 110 560 2.00e-117 357
MS.gene78663.t1 AT4G00190 54.019 311 138 4 191 500 165 471 5.54e-117 353
MS.gene78663.t1 AT5G51490 42.017 476 232 10 34 503 99 536 1.30e-115 352
MS.gene78663.t1 AT4G02300 42.355 484 234 8 28 503 86 532 2.92e-115 350
MS.gene78663.t1 AT3G10710 42.161 472 226 10 36 503 133 561 8.48e-115 350
MS.gene78663.t1 AT3G14310 53.727 322 145 2 183 503 274 592 3.85e-114 350
MS.gene78663.t1 AT5G27870 37.800 500 268 6 13 503 97 562 3.15e-110 343
MS.gene78663.t1 AT5G51500 50.149 335 159 4 172 503 211 540 6.17e-110 337
MS.gene78663.t1 AT2G26450 38.125 480 260 10 29 505 168 613 2.57e-108 335
MS.gene78663.t1 AT3G05610 36.863 510 274 7 1 503 97 565 3.29e-108 337
MS.gene78663.t1 AT3G59010 51.258 318 140 4 187 503 225 528 9.19e-108 331
MS.gene78663.t1 AT4G03930 51.592 314 146 3 191 503 216 524 1.60e-107 330
MS.gene78663.t1 AT3G27980 50.955 314 148 3 191 503 189 497 1.16e-106 327
MS.gene78663.t1 AT1G11590 50.955 314 148 3 191 503 216 524 1.72e-106 328
MS.gene78663.t1 AT1G53840 39.700 466 237 9 42 497 150 581 1.74e-106 330
MS.gene78663.t1 AT3G47400 41.580 481 241 9 29 503 148 594 1.79e-106 330
MS.gene78663.t1 AT5G49180 38.302 483 245 8 34 499 120 566 6.36e-104 323
MS.gene78663.t1 AT4G33230 40.444 450 247 8 57 505 179 608 1.55e-103 323
MS.gene78663.t1 AT5G20860 38.655 476 236 10 37 503 110 538 5.13e-101 314
MS.gene78663.t1 AT3G06830 37.179 468 275 5 34 500 116 565 6.72e-97 304
MS.gene78663.t1 AT3G14300 39.787 470 239 13 34 493 524 959 1.17e-96 313
MS.gene78663.t1 AT4G15980 40.849 377 215 4 127 499 326 698 3.33e-89 288
MS.gene78663.t1 AT4G15980 40.849 377 215 4 127 499 336 708 6.06e-89 287
MS.gene78663.t1 AT5G09760 45.541 314 158 5 187 491 237 546 2.24e-86 276
MS.gene78663.t1 AT5G09760 45.541 314 158 5 187 491 263 572 3.27e-86 277
MS.gene78663.t1 AT5G64640 43.614 321 165 6 187 495 285 601 4.64e-83 269
MS.gene78663.t1 AT1G11370 49.393 247 122 1 188 434 43 286 1.10e-82 258
MS.gene78663.t1 AT5G20860 34.430 456 215 9 37 483 110 490 2.95e-78 254
MS.gene78663.t1 AT2G47040 41.641 329 185 4 178 503 271 595 1.38e-75 249
MS.gene78663.t1 AT3G62170 43.175 315 172 4 192 503 278 588 1.58e-75 249
MS.gene78663.t1 AT2G47030 41.905 315 176 4 192 503 278 588 3.23e-75 248
MS.gene78663.t1 AT2G36710 33.758 314 187 5 191 497 91 390 1.47e-51 181
MS.gene78663.t1 AT5G19730 31.429 385 220 10 123 492 26 381 5.29e-51 179
MS.gene78663.t1 AT1G05310 34.161 322 182 6 189 497 89 393 2.07e-49 174
MS.gene78663.t1 AT3G17060 31.392 309 189 6 191 493 46 337 7.38e-47 166
MS.gene78663.t1 AT2G36700 32.459 305 186 5 193 492 44 333 8.03e-46 163
MS.gene78663.t1 AT3G29090 34.965 286 162 4 191 466 7 278 8.48e-46 163
MS.gene78663.t1 AT2G21610 32.450 302 184 6 191 492 56 337 1.42e-45 163
MS.gene78663.t1 AT2G21610 32.450 302 184 6 191 492 51 332 1.46e-45 163
MS.gene78663.t1 AT5G07430 33.546 313 179 9 188 492 64 355 2.92e-41 152
MS.gene78663.t1 AT1G44980 48.503 167 83 1 191 357 78 241 5.62e-41 147
MS.gene78663.t1 AT3G24130 30.882 340 202 9 170 498 17 334 7.21e-41 150
MS.gene78663.t1 AT5G47500 30.086 349 210 9 163 497 34 362 2.92e-39 146
MS.gene78663.t1 AT5G18990 33.449 287 164 6 191 466 29 299 1.29e-38 144
MS.gene78663.t1 AT5G55590 31.447 318 189 7 187 492 78 378 1.52e-37 142
MS.gene78663.t1 AT2G19150 32.372 312 181 9 191 492 41 332 4.63e-37 140
MS.gene78663.t1 AT5G55590 32.700 263 150 6 187 437 78 325 1.75e-35 135
MS.gene78663.t1 AT2G19150 33.463 257 143 8 191 437 41 279 1.01e-33 129
MS.gene78663.t1 AT5G07420 29.126 309 198 7 188 492 64 355 1.21e-31 125
MS.gene78663.t1 AT5G61680 29.412 306 192 9 193 492 71 358 7.07e-30 120
MS.gene78663.t1 AT2G47280 30.314 287 171 8 191 466 35 303 1.43e-29 119
MS.gene78663.t1 AT1G69940 27.759 299 190 10 201 492 76 355 6.97e-28 115
MS.gene78663.t1 AT5G07410 27.759 299 190 10 201 492 76 355 1.19e-27 114
MS.gene78663.t1 AT5G26810 27.616 344 217 10 168 498 13 337 7.21e-27 111
MS.gene78663.t1 AT3G49220 29.297 256 138 10 34 282 197 416 6.18e-14 74.3

Find 77 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGGTGAGTTTGATAATTA+TGG 0.151521 8.4:+56608019 MS.gene78663:CDS
TTGCATAATGCAGCTGTTTC+TGG 0.158204 8.4:+56607488 MS.gene78663:intron
GTTTATTTGCAATCATATAT+TGG 0.279888 8.4:+56607932 MS.gene78663:CDS
GAAATTCCTGTGTTCTGATT+TGG 0.280507 8.4:-56607809 None:intergenic
TCTTCTGCCTTCAAATTATC+AGG 0.287742 8.4:-56607009 None:intergenic
GAAACTGCAACGGTAGAATA+TGG 0.299226 8.4:-56607603 None:intergenic
GGTGATTTAATTCAACCTTC+TGG 0.303001 8.4:+56607953 MS.gene78663:CDS
CTGGTGCTATGACTCATAAT+AGG 0.323252 8.4:+56608044 MS.gene78663:CDS
TATGTTATTGTGAGTCTTGA+TGG 0.346098 8.4:+56606943 MS.gene78663:CDS
GATTGAAGCTGCAGATGATT+TGG 0.384083 8.4:+56607847 MS.gene78663:CDS
ACAGAAAACTACACTTCTAT+TGG 0.387214 8.4:+56606967 MS.gene78663:CDS
GAATTTCAATACAAAATTGT+AGG 0.393628 8.4:+56607825 MS.gene78663:CDS
GTGTAAGGTACATCTGTATC+AGG 0.398032 8.4:-56608154 None:intergenic
AACCTTCTGGATGGTTAGAA+TGG 0.401213 8.4:+56607966 MS.gene78663:CDS
GAGAAAGTCTTTGAATACTT+TGG 0.418166 8.4:-56604808 None:intergenic
CACCGATGTGTTGGTGGAAA+AGG 0.434972 8.4:+56604974 MS.gene78663:CDS
TACAGAGAATGTGACATCTA+TGG 0.441861 8.4:+56607671 MS.gene78663:CDS
AGCTGCACCTGATAATTTGA+AGG 0.445519 8.4:+56607002 MS.gene78663:CDS
AATTCAACTAAGAATTACTT+AGG 0.450056 8.4:+56607881 MS.gene78663:CDS
ATCGTCTAGTGCCGCAATCT+CGG 0.451993 8.4:-56604865 None:intergenic
TTTCTTATATATGTCAAAGA+AGG 0.454573 8.4:+56607039 MS.gene78663:CDS
TCATGTAGTCCTTCAGTGTA+AGG 0.455478 8.4:-56608169 None:intergenic
CTGACCTTGTAAGCCTTAGT+AGG 0.460092 8.4:-56605194 None:intergenic
GACCATGGAAAGTGTATTCA+AGG 0.469948 8.4:+56607906 MS.gene78663:CDS
AAGAAGAATATCTTGCTGAT+TGG 0.478621 8.4:+56607096 MS.gene78663:CDS
CAAACAAGGTCTTCCTACTA+AGG 0.479222 8.4:+56605181 MS.gene78663:CDS
TGTTTCTTACTGCTACTGCT+TGG 0.479557 8.4:-56607568 None:intergenic
TGGTGCTATGACTCATAATA+GGG 0.480312 8.4:+56608045 MS.gene78663:CDS
TGTAGAATTGTCTGAGGGAA+TGG 0.482417 8.4:-56607652 None:intergenic
GGAAATCATAGTGTGGTAGA+TGG 0.488932 8.4:+56607147 MS.gene78663:CDS
TAGAATGGAATGGAACAGTT+GGG 0.490562 8.4:+56607981 MS.gene78663:CDS
TGATGACTCCTACTCAAGCT+TGG 0.498547 8.4:+56608089 MS.gene78663:CDS
CCTTTCGTGTCTTATACAGT+TGG 0.500763 8.4:-56606878 None:intergenic
CAATTGTATAGTATTTCACT+TGG 0.503200 8.4:+56605110 MS.gene78663:CDS
ATCATAGTGTGGTAGATGGT+TGG 0.507535 8.4:+56607151 MS.gene78663:CDS
CTACATGATTTAGATAATTG+AGG 0.507887 8.4:+56608184 MS.gene78663:CDS
CTTAAAGAAAGTGAAAATAG+AGG 0.512875 8.4:+56606904 MS.gene78663:CDS
GTATAGTATTTCACTTGGGT+TGG 0.513615 8.4:+56605115 MS.gene78663:CDS
GATAATTTGAAGGCAGAAGA+TGG 0.515759 8.4:+56607012 MS.gene78663:CDS
TGTCAAAGAAGGAGTATATG+AGG 0.521664 8.4:+56607050 MS.gene78663:CDS
CTAAGTAATCTACATTGAGC+TGG 0.522297 8.4:-56604902 None:intergenic
CTCGGCATGGTTAAGAGAAG+TGG 0.523798 8.4:-56604847 None:intergenic
TCTTCCTACTAAGGCTTACA+AGG 0.530926 8.4:+56605190 MS.gene78663:CDS
ATTAACAAGACTTGCATCAC+AGG 0.533192 8.4:+56607126 MS.gene78663:CDS
TTCTACCGTTGCAGTTTCGA+AGG 0.533305 8.4:+56607608 MS.gene78663:CDS
GTCCTTGAATACACTTTCCA+TGG 0.545707 8.4:-56607908 None:intergenic
ATTTAATTCAACCTTCTGGA+TGG 0.546192 8.4:+56607957 MS.gene78663:CDS
TAACAACACCGATGTGTTGG+TGG 0.552935 8.4:+56604968 MS.gene78663:CDS
CAGCTGATTTAAGTTCATCT+CGG 0.554463 8.4:-56604932 None:intergenic
TCTGGATGGTTAGAATGGAA+TGG 0.564904 8.4:+56607971 MS.gene78663:CDS
TTAGAATGGAATGGAACAGT+TGG 0.566790 8.4:+56607980 MS.gene78663:CDS
AAAGGTTGAAGAATTAAAAG+TGG 0.571478 8.4:-56607175 None:intergenic
TCTTAACCATGCCGAGATTG+CGG 0.571735 8.4:+56604854 MS.gene78663:CDS
AAGCGTAAGACTCGCAAACA+AGG 0.581727 8.4:+56605167 MS.gene78663:CDS
TGGAGAGAGATTTATAGCAG+TGG 0.584956 8.4:+56607508 MS.gene78663:CDS
CTATTATGAGTCATAGCACC+AGG 0.589698 8.4:-56608043 None:intergenic
TTCCATTCTAACCATCCAGA+AGG 0.594789 8.4:-56607968 None:intergenic
GTGAAGTTCCAAGCTTGAGT+AGG 0.601017 8.4:-56608097 None:intergenic
AATGGACATAGAGTGTGTCT+TGG 0.606022 8.4:-56607634 None:intergenic
AATATCTTGCTGATTGGTGA+TGG 0.608985 8.4:+56607102 MS.gene78663:CDS
AATTGTATAGTATTTCACTT+GGG 0.612303 8.4:+56605111 MS.gene78663:CDS
TGAAACTTATCTTAGTGCTG+TGG 0.613599 8.4:+56604998 MS.gene78663:CDS
GAGTTTGATAATTATGGACC+TGG 0.614496 8.4:+56608025 MS.gene78663:CDS
ACAGATGTACCTTACACTGA+AGG 0.619292 8.4:+56608160 MS.gene78663:CDS
CATAATAGGGTACAATGGCA+TGG 0.619417 8.4:+56608058 MS.gene78663:CDS
TTCTCTGTAGAATTGTCTGA+GGG 0.620308 8.4:-56607657 None:intergenic
ATTCTCTGTAGAATTGTCTG+AGG 0.621472 8.4:-56607658 None:intergenic
CTAGTGCCGCAATCTCGGCA+TGG 0.621885 8.4:-56604860 None:intergenic
AGAATTACTTAGGAAGACCA+TGG 0.635888 8.4:+56607891 MS.gene78663:CDS
ACTGATCCAAATCAGAACAC+AGG 0.639047 8.4:+56607803 MS.gene78663:CDS
ATAACATTCAGAAACACAGC+AGG 0.642782 8.4:+56607533 MS.gene78663:CDS
GGTATCCTTCGAAACTGCAA+CGG 0.649403 8.4:-56607613 None:intergenic
TGACTCATAATAGGGTACAA+TGG 0.650167 8.4:+56608053 MS.gene78663:CDS
AATTGTGTAACATTACTCAA+CGG 0.661988 8.4:-56605092 None:intergenic
CATGGTTAAGAGAAGTGGAG+TGG 0.677604 8.4:-56604842 None:intergenic
CATCACAGGAAATCATAGTG+TGG 0.687415 8.4:+56607140 MS.gene78663:CDS
CCAACTGTATAAGACACGAA+AGG 0.694183 8.4:+56606878 MS.gene78663:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAGTTAATAATTTAAATA+TGG + chr8.4:56606032-56606051 MS.gene78663:intron 10.0%
!! ATATTTAATGAAACAAAATA+AGG - chr8.4:56605275-56605294 None:intergenic 10.0%
!! TCAAAAATAAATATTTGTAA+GGG - chr8.4:56605339-56605358 None:intergenic 10.0%
!! AAAAATTCATATGAATATGA+TGG + chr8.4:56605954-56605973 MS.gene78663:intron 15.0%
!! AACTAAAACAAATAAAAAGA+AGG + chr8.4:56605978-56605997 MS.gene78663:intron 15.0%
!! ACATAAATTAAATGCATTTA+AGG - chr8.4:56606700-56606719 None:intergenic 15.0%
!! ACTAAAACAAATAAAAAGAA+GGG + chr8.4:56605979-56605998 MS.gene78663:intron 15.0%
!! ATTAAATGCATAAAATTGTA+AGG + chr8.4:56606107-56606126 MS.gene78663:intron 15.0%
!! CTCAAAAATAAATATTTGTA+AGG - chr8.4:56605340-56605359 None:intergenic 15.0%
!! TAAATATGGAAATAATTCAT+TGG + chr8.4:56606046-56606065 MS.gene78663:intron 15.0%
!! TTAATTTAGTTACATTATGA+AGG + chr8.4:56605576-56605595 MS.gene78663:intron 15.0%
!!! AAATATATGTGTTCATTTTA+TGG + chr8.4:56605603-56605622 MS.gene78663:intron 15.0%
!!! TAAGAAAATATTTCCATTTT+CGG - chr8.4:56606239-56606258 None:intergenic 15.0%
!! AATTCAACTAAGAATTACTT+AGG + chr8.4:56607881-56607900 MS.gene78663:CDS 20.0%
!! AATTGTATAGTATTTCACTT+GGG + chr8.4:56605111-56605130 MS.gene78663:CDS 20.0%
!! ATAACGTAAAATATGTTAAC+TGG + chr8.4:56606437-56606456 MS.gene78663:intron 20.0%
!! ATTTAACAGTAACATTATGA+AGG + chr8.4:56606766-56606785 MS.gene78663:intron 20.0%
!! ATTTATAAAGTAATGCTCTT+GGG - chr8.4:56605403-56605422 None:intergenic 20.0%
!! GAATTTCAATACAAAATTGT+AGG + chr8.4:56607825-56607844 MS.gene78663:CDS 20.0%
!! GTTTATTTGCAATCATATAT+TGG + chr8.4:56607932-56607951 MS.gene78663:CDS 20.0%
!! TGCATTATGCAAATAAATTA+AGG - chr8.4:56607480-56607499 None:intergenic 20.0%
!! TTATGCAAATAAATTAAGGT+CGG - chr8.4:56607476-56607495 None:intergenic 20.0%
!! TTGTAACAAAACAAAAGTAA+CGG - chr8.4:56605859-56605878 None:intergenic 20.0%
!! TTTCTTATATATGTCAAAGA+AGG + chr8.4:56607039-56607058 MS.gene78663:CDS 20.0%
!!! AAGAAAATATTTCCATTTTC+GGG - chr8.4:56606238-56606257 None:intergenic 20.0%
!!! AATCATTATACTTGTTTTGA+TGG + chr8.4:56605026-56605045 MS.gene78663:CDS 20.0%
!!! ATCATTATACTTGTTTTGAT+GGG + chr8.4:56605027-56605046 MS.gene78663:CDS 20.0%
!!! TGTAAGGAATATTTTTCTTT+TGG + chr8.4:56606123-56606142 MS.gene78663:intron 20.0%
! AAAGGTTGAAGAATTAAAAG+TGG - chr8.4:56607178-56607197 None:intergenic 25.0%
! AAATATAACATCTCACATCT+CGG + chr8.4:56607339-56607358 MS.gene78663:intron 25.0%
! AATTGTGTAACATTACTCAA+CGG - chr8.4:56605095-56605114 None:intergenic 25.0%
! ACGCAAATTAAATGTTTAGT+GGG + chr8.4:56606410-56606429 MS.gene78663:intron 25.0%
! AGCATTACTTTATAAATGTG+AGG + chr8.4:56605406-56605425 MS.gene78663:intron 25.0%
! ATTATATACAAGATGACAAG+TGG - chr8.4:56606643-56606662 None:intergenic 25.0%
! CAATTGTATAGTATTTCACT+TGG + chr8.4:56605110-56605129 MS.gene78663:CDS 25.0%
! CATTTATAAAGTAATGCTCT+TGG - chr8.4:56605404-56605423 None:intergenic 25.0%
! CTTAAAGAAAGTGAAAATAG+AGG + chr8.4:56606904-56606923 MS.gene78663:CDS 25.0%
! TAACACTACCATAAAAAGAA+GGG + chr8.4:56606176-56606195 MS.gene78663:intron 25.0%
! TACGCAAATTAAATGTTTAG+TGG + chr8.4:56606409-56606428 MS.gene78663:intron 25.0%
! TTAACACTACCATAAAAAGA+AGG + chr8.4:56606175-56606194 MS.gene78663:intron 25.0%
!! ACTGTGCTTAATTTTACATT+GGG + chr8.4:56608118-56608137 MS.gene78663:CDS 25.0%
!! CTGACAATTTGTTTATAAGT+GGG - chr8.4:56605776-56605795 None:intergenic 25.0%
!! CTTGGGTTATTGATTAAAAA+TGG - chr8.4:56605386-56605405 None:intergenic 25.0%
!! GAAAAAATTTGACTTACCAA+AGG - chr8.4:56607196-56607215 None:intergenic 25.0%
!! GTTCGTATATATGCTTTATT+AGG - chr8.4:56607258-56607277 None:intergenic 25.0%
!! TATGGTGAGTTTGATAATTA+TGG + chr8.4:56608019-56608038 MS.gene78663:CDS 25.0%
!! TGACAATTTGTTTATAAGTG+GGG - chr8.4:56605775-56605794 None:intergenic 25.0%
!!! AAGAAGAAGTTGATATAACT+GGG - chr8.4:56606615-56606634 None:intergenic 25.0%
!!! ACCTTAATAAGCTTTTTAAG+TGG - chr8.4:56605224-56605243 None:intergenic 25.0%
!!! ACTTTTAATTCTTCAACCTT+TGG + chr8.4:56607177-56607196 MS.gene78663:CDS 25.0%
!!! GAATACTTTGGATAGTTTTT+TGG - chr8.4:56604799-56604818 None:intergenic 25.0%
!!! TAAGAAGAAGTTGATATAAC+TGG - chr8.4:56606616-56606635 None:intergenic 25.0%
AAAAATGCTATAACAGCACA+AGG + chr8.4:56607776-56607795 MS.gene78663:CDS 30.0%
AAGAAGAATATCTTGCTGAT+TGG + chr8.4:56607096-56607115 MS.gene78663:CDS 30.0%
ACAGAAAACTACACTTCTAT+TGG + chr8.4:56606967-56606986 MS.gene78663:CDS 30.0%
ATTTAATTCAACCTTCTGGA+TGG + chr8.4:56607957-56607976 MS.gene78663:CDS 30.0%
CATTTGTCACATTGTGAATA+AGG - chr8.4:56605492-56605511 None:intergenic 30.0%
CCACTTATAAACAAATTGTC+AGG + chr8.4:56605774-56605793 MS.gene78663:intron 30.0%
GAGAAAGTCTTTGAATACTT+TGG - chr8.4:56604811-56604830 None:intergenic 30.0%
GCCACTTAAAAAGCTTATTA+AGG + chr8.4:56605220-56605239 MS.gene78663:CDS 30.0%
TATGTTATTGTGAGTCTTGA+TGG + chr8.4:56606943-56606962 MS.gene78663:CDS 30.0%
TTAGAGTCTCTTCTTCTAAA+AGG + chr8.4:56605681-56605700 MS.gene78663:intron 30.0%
TTTGTTTGTTTGTGCCATAA+TGG + chr8.4:56606555-56606574 MS.gene78663:intron 30.0%
! ATGTGTTCATTTTATGGCTA+AGG + chr8.4:56605609-56605628 MS.gene78663:intron 30.0%
! ATTTTACATTGGGGAATACT+TGG + chr8.4:56608128-56608147 MS.gene78663:CDS 30.0%
! ATTTTTACTCAAAGCTTGAG+TGG - chr8.4:56605141-56605160 None:intergenic 30.0%
! CACTGTGCTTAATTTTACAT+TGG + chr8.4:56608117-56608136 MS.gene78663:CDS 30.0%
! CATTTTTGCAAGAAAACCAA+TGG + chr8.4:56607745-56607764 MS.gene78663:CDS 30.0%
! CCTGACAATTTGTTTATAAG+TGG - chr8.4:56605777-56605796 None:intergenic 30.0%
! CTGTGCTTAATTTTACATTG+GGG + chr8.4:56608119-56608138 MS.gene78663:CDS 30.0%
! GACAATTTGTTTATAAGTGG+GGG - chr8.4:56605774-56605793 None:intergenic 30.0%
! GGGTTAGATACACTATTTTA+TGG + chr8.4:56608001-56608020 MS.gene78663:CDS 30.0%
! TAACATGACCCTTCTTTTTA+TGG - chr8.4:56606187-56606206 None:intergenic 30.0%
!! AATCATTAATTTGTGCCTTG+AGG + chr8.4:56606333-56606352 MS.gene78663:intron 30.0%
!! AATTATTAACTTGTGCCTTG+AGG - chr8.4:56606026-56606045 None:intergenic 30.0%
!! AGAAGAAGTTGATATAACTG+GGG - chr8.4:56606614-56606633 None:intergenic 30.0%
!! ATCATTAATTTGTGCCTTGA+GGG + chr8.4:56606334-56606353 MS.gene78663:intron 30.0%
!! ATTATTAACTTGTGCCTTGA+GGG - chr8.4:56606025-56606044 None:intergenic 30.0%
!!! AATCCTCGCATTTTTTTTTG+TGG - chr8.4:56605889-56605908 None:intergenic 30.0%
!!! ATAGTTTTTTGGCTTGTTTG+AGG - chr8.4:56604788-56604807 None:intergenic 30.0%
!!! GCATTTTTTTGATTTGCCAT+TGG - chr8.4:56607764-56607783 None:intergenic 30.0%
!!! TTATACTTGTTTTGATGGGT+TGG + chr8.4:56605031-56605050 MS.gene78663:CDS 30.0%
AAACTCTTAGAAATCGTGGT+TGG + chr8.4:56605705-56605724 MS.gene78663:intron 35.0%
AACTCTTAGAAATCGTGGTT+GGG + chr8.4:56605706-56605725 MS.gene78663:intron 35.0%
AAGTTAGCTAAAACCTAGGT+TGG - chr8.4:56606489-56606508 None:intergenic 35.0%
ACACCACAAAAAAAAATGCG+AGG + chr8.4:56605883-56605902 MS.gene78663:intron 35.0%
AGAATTACTTAGGAAGACCA+TGG + chr8.4:56607891-56607910 MS.gene78663:CDS 35.0%
ATAACATTCAGAAACACAGC+AGG + chr8.4:56607533-56607552 MS.gene78663:CDS 35.0%
ATCTCGAAAGTCTTACATCA+TGG + chr8.4:56605548-56605567 MS.gene78663:intron 35.0%
ATTAACAAGACTTGCATCAC+AGG + chr8.4:56607126-56607145 MS.gene78663:CDS 35.0%
ATTCTCTGTAGAATTGTCTG+AGG - chr8.4:56607661-56607680 None:intergenic 35.0%
ATTTCTTAACATGTGCCCTA+GGG + chr8.4:56606146-56606165 MS.gene78663:intron 35.0%
CTAAGTAATCTACATTGAGC+TGG - chr8.4:56604905-56604924 None:intergenic 35.0%
CTTCAGATTAAAGTCGTATC+TGG - chr8.4:56607393-56607412 None:intergenic 35.0%
GAAATTCCTGTGTTCTGATT+TGG - chr8.4:56607812-56607831 None:intergenic 35.0%
GATAATTTGAAGGCAGAAGA+TGG + chr8.4:56607012-56607031 MS.gene78663:CDS 35.0%
GGTGATTTAATTCAACCTTC+TGG + chr8.4:56607953-56607972 MS.gene78663:CDS 35.0%
GTAAGACTTTCGAGATTTCA+AGG - chr8.4:56605544-56605563 None:intergenic 35.0%
GTATAGTATTTCACTTGGGT+TGG + chr8.4:56605115-56605134 MS.gene78663:CDS 35.0%
GTTAATGATGTGTACGTGTT+AGG - chr8.4:56607296-56607315 None:intergenic 35.0%
TACAGAGAATGTGACATCTA+TGG + chr8.4:56607671-56607690 MS.gene78663:CDS 35.0%
TAGAATGGAATGGAACAGTT+GGG + chr8.4:56607981-56608000 MS.gene78663:CDS 35.0%
TCAGAAGTTAGCTAAAACCT+AGG - chr8.4:56606493-56606512 None:intergenic 35.0%
TCTCGAAAGTCTTACATCAT+GGG + chr8.4:56605549-56605568 MS.gene78663:intron 35.0%
TCTTCTGCCTTCAAATTATC+AGG - chr8.4:56607012-56607031 None:intergenic 35.0%
TGAAACTTATCTTAGTGCTG+TGG + chr8.4:56604998-56605017 MS.gene78663:CDS 35.0%
TGACTCATAATAGGGTACAA+TGG + chr8.4:56608053-56608072 MS.gene78663:CDS 35.0%
TGTGAGGATTAAAATGAGCT+TGG + chr8.4:56605422-56605441 MS.gene78663:intron 35.0%
TTAGAATGGAATGGAACAGT+TGG + chr8.4:56607980-56607999 MS.gene78663:CDS 35.0%
TTCTCTGTAGAATTGTCTGA+GGG - chr8.4:56607660-56607679 None:intergenic 35.0%
TTGTGTTAAGAGTCCCATAT+CGG - chr8.4:56605811-56605830 None:intergenic 35.0%
! AATATCTTGCTGATTGGTGA+TGG + chr8.4:56607102-56607121 MS.gene78663:CDS 35.0%
! ACCTCACAAGTCAATTTTGT+GGG - chr8.4:56605743-56605762 None:intergenic 35.0%
! ACGACTTTAATCTGAAGTGT+CGG + chr8.4:56607396-56607415 MS.gene78663:intron 35.0%
! CAATTTTGTGGGGTTAAGTT+AGG - chr8.4:56605732-56605751 None:intergenic 35.0%
! CAGCTGATTTAAGTTCATCT+CGG - chr8.4:56604935-56604954 None:intergenic 35.0%
! GAGTTTGATAATTATGGACC+TGG + chr8.4:56608025-56608044 MS.gene78663:CDS 35.0%
! TGTCAAAGAAGGAGTATATG+AGG + chr8.4:56607050-56607069 MS.gene78663:CDS 35.0%
! TTTTAACAACACCGATGTGT+TGG + chr8.4:56604965-56604984 MS.gene78663:CDS 35.0%
!! GGCACAGTTGATTTCATTTT+CGG + chr8.4:56607692-56607711 MS.gene78663:CDS 35.0%
!! TGGTGCTATGACTCATAATA+GGG + chr8.4:56608045-56608064 MS.gene78663:CDS 35.0%
AACCTTCTGGATGGTTAGAA+TGG + chr8.4:56607966-56607985 MS.gene78663:CDS 40.0%
AAGAGACTCTAAACTAGCGA+GGG - chr8.4:56605673-56605692 None:intergenic 40.0%
AATGGACATAGAGTGTGTCT+TGG - chr8.4:56607637-56607656 None:intergenic 40.0%
ACAGATGTACCTTACACTGA+AGG + chr8.4:56608160-56608179 MS.gene78663:CDS 40.0%
ACTGATCCAAATCAGAACAC+AGG + chr8.4:56607803-56607822 MS.gene78663:CDS 40.0%
AGCTGCACCTGATAATTTGA+AGG + chr8.4:56607002-56607021 MS.gene78663:CDS 40.0%
AGGCAAACTCTTAGAAATCG+TGG + chr8.4:56605701-56605720 MS.gene78663:intron 40.0%
ATCATAGTGTGGTAGATGGT+TGG + chr8.4:56607151-56607170 MS.gene78663:CDS 40.0%
ATGTTAACTGGCTAGATCCA+AGG + chr8.4:56606449-56606468 MS.gene78663:intron 40.0%
CAAACAAGGTCTTCCTACTA+AGG + chr8.4:56605181-56605200 MS.gene78663:CDS 40.0%
CATAATAGGGTACAATGGCA+TGG + chr8.4:56608058-56608077 MS.gene78663:CDS 40.0%
CATCACAGGAAATCATAGTG+TGG + chr8.4:56607140-56607159 MS.gene78663:CDS 40.0%
CATGTTAAGCAACCCGAAAA+TGG + chr8.4:56606223-56606242 MS.gene78663:intron 40.0%
CCAACTGTATAAGACACGAA+AGG + chr8.4:56606878-56606897 MS.gene78663:CDS 40.0%
CCTTTCGTGTCTTATACAGT+TGG - chr8.4:56606881-56606900 None:intergenic 40.0%
CTATTATGAGTCATAGCACC+AGG - chr8.4:56608046-56608065 None:intergenic 40.0%
CTGGTGTTCAACATATGTGT+TGG - chr8.4:56607374-56607393 None:intergenic 40.0%
GAAACTGCAACGGTAGAATA+TGG - chr8.4:56607606-56607625 None:intergenic 40.0%
GATCCAAGGAGGTTAAATTC+TGG + chr8.4:56606463-56606482 MS.gene78663:intron 40.0%
GATTGAAGCTGCAGATGATT+TGG + chr8.4:56607847-56607866 MS.gene78663:CDS 40.0%
GATTTCTTAACATGTGCCCT+AGG + chr8.4:56606145-56606164 MS.gene78663:intron 40.0%
GGAAATCATAGTGTGGTAGA+TGG + chr8.4:56607147-56607166 MS.gene78663:CDS 40.0%
GTCCTTGAATACACTTTCCA+TGG - chr8.4:56607911-56607930 None:intergenic 40.0%
GTGTAAGGTACATCTGTATC+AGG - chr8.4:56608157-56608176 None:intergenic 40.0%
GTTTCCAAACAACACTCTTG+CGG - chr8.4:56605463-56605482 None:intergenic 40.0%
TCATGTAGTCCTTCAGTGTA+AGG - chr8.4:56608172-56608191 None:intergenic 40.0%
TCGAGATTTCAAGGAACACA+GGG - chr8.4:56605535-56605554 None:intergenic 40.0%
TCTGGATGGTTAGAATGGAA+TGG + chr8.4:56607971-56607990 MS.gene78663:CDS 40.0%
TCTTCCTACTAAGGCTTACA+AGG + chr8.4:56605190-56605209 MS.gene78663:CDS 40.0%
TGCACGTGTTCTTTCCATTA+TGG - chr8.4:56606572-56606591 None:intergenic 40.0%
TGGAGAGAGATTTATAGCAG+TGG + chr8.4:56607508-56607527 MS.gene78663:CDS 40.0%
TGTAGAATTGTCTGAGGGAA+TGG - chr8.4:56607655-56607674 None:intergenic 40.0%
TGTTTCTTACTGCTACTGCT+TGG - chr8.4:56607571-56607590 None:intergenic 40.0%
TTCCATTCTAACCATCCAGA+AGG - chr8.4:56607971-56607990 None:intergenic 40.0%
TTCGAGATTTCAAGGAACAC+AGG - chr8.4:56605536-56605555 None:intergenic 40.0%
! AACCTTTTCCACCAACACAT+CGG - chr8.4:56604979-56604998 None:intergenic 40.0%
! CACCTCACAAGTCAATTTTG+TGG - chr8.4:56605744-56605763 None:intergenic 40.0%
! CCTCACAAGTCAATTTTGTG+GGG - chr8.4:56605742-56605761 None:intergenic 40.0%
! GACCATGGAAAGTGTATTCA+AGG + chr8.4:56607906-56607925 MS.gene78663:CDS 40.0%
! TGATTTCATTTTCGGCAACG+CGG + chr8.4:56607700-56607719 MS.gene78663:CDS 40.0%
! TTGCATAATGCAGCTGTTTC+TGG + chr8.4:56607488-56607507 MS.gene78663:intron 40.0%
!! AAGTTGATATAACTGGGGTG+GGG - chr8.4:56606609-56606628 None:intergenic 40.0%
!! AGAAGTTGATATAACTGGGG+TGG - chr8.4:56606611-56606630 None:intergenic 40.0%
!! CAAAATTGACTTGTGAGGTG+AGG + chr8.4:56605744-56605763 MS.gene78663:intron 40.0%
!! CTGGTGCTATGACTCATAAT+AGG + chr8.4:56608044-56608063 MS.gene78663:CDS 40.0%
!! GAAGTTGATATAACTGGGGT+GGG - chr8.4:56606610-56606629 None:intergenic 40.0%
!! GTCATGTTAACTTGTGCCTA+GGG + chr8.4:56606198-56606217 MS.gene78663:intron 40.0%
!! TAAGTTAACTTGTGCCCTCA+AGG - chr8.4:56606351-56606370 None:intergenic 40.0%
!! TAGTGTTAACTTGTGCCCTA+GGG - chr8.4:56606164-56606183 None:intergenic 40.0%
!! TTAACCGCAAGAGTGTTGTT+TGG + chr8.4:56605456-56605475 MS.gene78663:intron 40.0%
AAGCGTAAGACTCGCAAACA+AGG + chr8.4:56605167-56605186 MS.gene78663:CDS 45.0%
AGTGAGATGAGTGAGCAGAA+TGG - chr8.4:56605642-56605661 None:intergenic 45.0%
ATTCTGCTCACTCATCTCAC+TGG + chr8.4:56605641-56605660 MS.gene78663:intron 45.0%
CATGGTTAAGAGAAGTGGAG+TGG - chr8.4:56604845-56604864 None:intergenic 45.0%
CGAGATTTCAAGGAACACAG+GGG - chr8.4:56605534-56605553 None:intergenic 45.0%
CTGACCTTGTAAGCCTTAGT+AGG - chr8.4:56605197-56605216 None:intergenic 45.0%
GAAGAGACTCTAAACTAGCG+AGG - chr8.4:56605674-56605693 None:intergenic 45.0%
GGTATCCTTCGAAACTGCAA+CGG - chr8.4:56607616-56607635 None:intergenic 45.0%
GGTTAAATTCTGGCCAACCT+AGG + chr8.4:56606473-56606492 MS.gene78663:intron 45.0%
GTGAAGTTCCAAGCTTGAGT+AGG - chr8.4:56608100-56608119 None:intergenic 45.0%
TAACAACACCGATGTGTTGG+TGG + chr8.4:56604968-56604987 MS.gene78663:CDS 45.0%
TCTTAACCATGCCGAGATTG+CGG + chr8.4:56604854-56604873 MS.gene78663:CDS 45.0%
TGAGTAGTATCTCAGTCACG+TGG + chr8.4:56606510-56606529 MS.gene78663:intron 45.0%
TGATGACTCCTACTCAAGCT+TGG + chr8.4:56608089-56608108 MS.gene78663:CDS 45.0%
TGGCCAGAATTTAACCTCCT+TGG - chr8.4:56606469-56606488 None:intergenic 45.0%
TTAACTGGCTAGATCCAAGG+AGG + chr8.4:56606452-56606471 MS.gene78663:intron 45.0%
TTAAGAGTCCCATATCGGAC+AGG - chr8.4:56605806-56605825 None:intergenic 45.0%
TTCTACCGTTGCAGTTTCGA+AGG + chr8.4:56607608-56607627 MS.gene78663:CDS 45.0%
! CGTGTTAGGTGTCATATGTC+TGG - chr8.4:56607282-56607301 None:intergenic 45.0%
! TAACTTGTATCCTGCCCTCA+AGG + chr8.4:56606008-56606027 MS.gene78663:intron 45.0%
!! CCCCACAAAATTGACTTGTG+AGG + chr8.4:56605739-56605758 MS.gene78663:intron 45.0%
!! GGTCATGTTAACTTGTGCCT+AGG + chr8.4:56606197-56606216 MS.gene78663:intron 45.0%
!! GTAGTGTTAACTTGTGCCCT+AGG - chr8.4:56606165-56606184 None:intergenic 45.0%
!! TATAAAGAATAAATATTAAA+CGG + chr8.4:56605926-56605945 MS.gene78663:intron 5.0%
ATCGTCTAGTGCCGCAATCT+CGG - chr8.4:56604868-56604887 None:intergenic 50.0%
CTCGGCATGGTTAAGAGAAG+TGG - chr8.4:56604850-56604869 None:intergenic 50.0%
GGTTGCTTAACATGTGCCCT+AGG - chr8.4:56606217-56606236 None:intergenic 50.0%
TTGATATAACTGGGGTGGGG+TGG - chr8.4:56606606-56606625 None:intergenic 50.0%
! CACCGATGTGTTGGTGGAAA+AGG + chr8.4:56604974-56604993 MS.gene78663:CDS 50.0%
!! TTAACTTGTGCCTTGAGGGC+AGG - chr8.4:56606021-56606040 None:intergenic 50.0%
!!! GCTACTGCTTGGTGCTTTTG+AGG - chr8.4:56607560-56607579 None:intergenic 50.0%
AGGCCTTCTCCTGTCCGATA+TGG + chr8.4:56605794-56605813 MS.gene78663:intron 55.0%
GGCCTTCTCCTGTCCGATAT+GGG + chr8.4:56605795-56605814 MS.gene78663:intron 55.0%
GTCCCATATCGGACAGGAGA+AGG - chr8.4:56605800-56605819 None:intergenic 55.0%
CTAGTGCCGCAATCTCGGCA+TGG - chr8.4:56604863-56604882 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 56604774 56608204 56604774 ID=MS.gene78663
chr8.4 mRNA 56604774 56608204 56604774 ID=MS.gene78663.t1;Parent=MS.gene78663
chr8.4 exon 56604774 56605241 56604774 ID=MS.gene78663.t1.exon1;Parent=MS.gene78663.t1
chr8.4 CDS 56604774 56605241 56604774 ID=cds.MS.gene78663.t1;Parent=MS.gene78663.t1
chr8.4 exon 56606844 56607198 56606844 ID=MS.gene78663.t1.exon2;Parent=MS.gene78663.t1
chr8.4 CDS 56606844 56607198 56606844 ID=cds.MS.gene78663.t1;Parent=MS.gene78663.t1
chr8.4 exon 56607501 56608204 56607501 ID=MS.gene78663.t1.exon3;Parent=MS.gene78663.t1
chr8.4 CDS 56607501 56608204 56607501 ID=cds.MS.gene78663.t1;Parent=MS.gene78663.t1
Gene Sequence

>MS.gene78663

ATGAAACAAAGCCTCAAACAAGCCAAAAAACTATCCAAAGTATTCAAAGACTTTCTCAAAAAACATCACCACTCCACTTCTCTTAACCATGCCGAGATTGCGGCACTAGACGATTGTAGTGAACTAAACCAGCTCAATGTAGATTACTTAGAGTTAGTCCGAGATGAACTTAAATCAGCTGATTCTTCATCTTTTAACAACACCGATGTGTTGGTGGAAAAGGTTGAAACTTATCTTAGTGCTGTGGCTACTAATCATTATACTTGTTTTGATGGGTTGGTTGTGACTAAAAGTAACATTGCTAATGCACTTGCTGTGCCGTTGAGTAATGTTACACAATTGTATAGTATTTCACTTGGGTTGGCCACTCAAGCTTTGAGTAAAAATATTAAGAAGCGTAAGACTCGCAAACAAGGTCTTCCTACTAAGGCTTACAAGGTCAGACAGCCACTTAAAAAGCTTATTAAGGTAACTATTTTCTTTATCTATATTTGACTACCTTATTTTGTTTCATTAAATATGTTTTTTTTTGTCAAAAAAAAAAATTAAATATGTTTTTTAGCCCTTACAAATATTTATTTTTGAGTTTGATTTCTATAAGTATTTTATCCATTTTTAATCAATAACCCAAGAGCATTACTTTATAAATGTGAGGATTAAAATGAGCTTGGCTCTACAAACTTTAACCGCAAGAGTGTTGTTTGGAAACAGTAGACCTTATTCACAATGTGACAAATGCAAAGTTTGCATGTGTATGCCCCTGTGTTCCTTGAAATCTCGAAAGTCTTACATCATGGGATTATTAATTTAGTTACATTATGAAGGAGCAAAATATATGTGTTCATTTTATGGCTAAGGATCGTGTCCATTCTGCTCACTCATCTCACTGGAATCATCCCTCGCTAGTTTAGAGTCTCTTCTTCTAAAAGGCAAACTCTTAGAAATCGTGGTTGGGCCTAACTTAACCCCACAAAATTGACTTGTGAGGTGAGGATTGCCCCCACTTATAAACAAATTGTCAGGCCTTCTCCTGTCCGATATGGGACTCTTAACACAAACTAGTTATTTAGTTTTTTTTTCTTCCGTTACTTTTGTTTTGTTACAATGTAACACCACAAAAAAAAATGCGAGGATTAAAATAGATAATTTTTTTATAAAGAATAAATATTAAACGGCGATCAAAAATTCATATGAATATGATGGTAACTAAAACAAATAAAAAGAAGGGAAATATTAACTTGTATCCTGCCCTCAAGGCACAAGTTAATAATTTAAATATGGAAATAATTCATTGGAATGTGTGCATTGCATTTAATCTTTAACTTTTTAATCTATTAAATGCATAAAATTGTAAGGAATATTTTTCTTTTGGATTTCTTAACATGTGCCCTAGGGCACAAGTTAACACTACCATAAAAAGAAGGGTCATGTTAACTTGTGCCTAGGGCACATGTTAAGCAACCCGAAAATGGAAATATTTTCTTAAATTTTGTGCATTCAATTATTGAAAAGTAAAAAAGTTAAAGTCATATACATTAATTACAAATATTTCTATTTTGAATCATTAATTTGTGCCTTGAGGGCACAAGTTAACTTATATTGTTGTGTTATCGAAATCTAATCAGCACTTAGTCATACGCAAATTAAATGTTTAGTGGGATTAATAACGTAAAATATGTTAACTGGCTAGATCCAAGGAGGTTAAATTCTGGCCAACCTAGGTTTTAGCTAACTTCTGAGTAGTATCTCAGTCACGTGGTTTCATGTTCGTTAATCGTTTGTTTGTTTGTTTGTGCCATAATGGAAAGAACACGTGCAACTCACAAGTACCACCCCACCCCAGTTATATCAACTTCTTCTTATATGCCACTTGTCATCTTGTATATAATATGTTCGAAAAAAATTAAAAAACAATTACTTTGTCCTTAAATGCATTTAATTTATGTGTAATGACATTTGAAGCTCATCATAGAAAAGTAATGATAATTATGCATTTAACAGTAACATTATGAAGGTAAAATTGTTAATTGAAATTTGAAAGTCTAAAATTGTAACATTACTCTATTGCAGCTTCTTCACACAAAACACAGTTGCAATAACTCATCCAACTGTATAAGACACGAAAGGATTCTTAAAGAAAGTGAAAATAGAGGAATTTTACTGAAAGAATATGTTATTGTGAGTCTTGATGGAACAGAAAACTACACTTCTATTGGTGAAGCTATTGAAGCTGCACCTGATAATTTGAAGGCAGAAGATGGCTATTTTCTTATATATGTCAAAGAAGGAGTATATGAGGAATATGTTACTATTACAAAACATAAGAAGAATATCTTGCTGATTGGTGATGGTATTAACAAGACTTGCATCACAGGAAATCATAGTGTGGTAGATGGTTGGACCACTTTTAATTCTTCAACCTTTGGTAAGTCAAATTTTTTCAACTATACTAGAGATTTTTCAATAAAAAGTTAACTATAACCTAATAAAGCATATATACGAACACCAGACATATGACACCTAACACGTACACATCATTAACAAGTTAAGAAAATTAAAGTTATTAAATATAACATCTCACATCTCGGTATTAGACACCAACACATATGTTGAACACCAGATACGACTTTAATCTGAAGTGTCGGTACTACATAACATAGAGTGCAATCATGATAGAACGTTATTTTTCTTGTAATAATCCGACCTTAATTTATTTGCATAATGCAGCTGTTTCTGGAGAGAGATTTATAGCAGTGGACATAACATTCAGAAACACAGCAGGACCTCAAAAGCACCAAGCAGTAGCAGTAAGAAACAATGCAGATCTTTCCATATTCTACCGTTGCAGTTTCGAAGGATACCAAGACACACTCTATGTCCATTCCCTCAGACAATTCTACAGAGAATGTGACATCTATGGCACAGTTGATTTCATTTTCGGCAACGCGGCTGCAGTACTTCAATCTTGCAACATTTTTGCAAGAAAACCAATGGCAAATCAAAAAAATGCTATAACAGCACAAGGTAGAACTGATCCAAATCAGAACACAGGAATTTCAATACAAAATTGTAGGATTGAAGCTGCAGATGATTTGGCTGAAGATTTGAATTCAACTAAGAATTACTTAGGAAGACCATGGAAAGTGTATTCAAGGACTGTTTATTTGCAATCATATATTGGTGATTTAATTCAACCTTCTGGATGGTTAGAATGGAATGGAACAGTTGGGTTAGATACACTATTTTATGGTGAGTTTGATAATTATGGACCTGGTGCTATGACTCATAATAGGGTACAATGGCATGGTTACAATTTGATGACTCCTACTCAAGCTTGGAACTTCACTGTGCTTAATTTTACATTGGGGAATACTTGGCTTCCTGATACAGATGTACCTTACACTGAAGGACTACATGATTTAGATAATTGA
Protein sequence

>MS.gene78663.t1

MKQSLKQAKKLSKVFKDFLKKHHHSTSLNHAEIAALDDCSELNQLNVDYLELVRDELKSADSSSFNNTDVLVEKVETYLSAVATNHYTCFDGLVVTKSNIANALAVPLSNVTQLYSISLGLATQALSKNIKKRKTRKQGLPTKAYKVRQPLKKLIKLLHTKHSCNNSSNCIRHERILKESENRGILLKEYVIVSLDGTENYTSIGEAIEAAPDNLKAEDGYFLIYVKEGVYEEYVTITKHKKNILLIGDGINKTCITGNHSVVDGWTTFNSSTFAVSGERFIAVDITFRNTAGPQKHQAVAVRNNADLSIFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVLQSCNIFARKPMANQKNAITAQGRTDPNQNTGISIQNCRIEAADDLAEDLNSTKNYLGRPWKVYSRTVYLQSYIGDLIQPSGWLEWNGTVGLDTLFYGEFDNYGPGAMTHNRVQWHGYNLMTPTQAWNFTVLNFTLGNTWLPDTDVPYTEGLHDLDN