AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene79738


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79738.t1 MTR_8g028015 99.541 218 1 0 1 218 1 218 6.84e-154 425
MS.gene79738.t1 MTR_8g079890 52.344 128 59 2 1 127 1 127 1.29e-40 137
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79738.t1 AT5G12230 63.229 223 75 3 1 218 1 221 3.60e-61 191
MS.gene79738.t1 AT5G19480 60.000 215 77 3 1 214 1 207 2.23e-56 178
MS.gene79738.t1 AT5G19480 60.000 215 77 3 1 214 1 207 2.23e-56 178
MS.gene79738.t1 AT5G19480 60.000 215 77 3 1 214 1 207 2.23e-56 178
MS.gene79738.t1 AT5G19480 60.000 215 77 3 1 214 1 207 2.23e-56 178

Find 40 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGGAGCTGTTTCCCTTAGT+TGG 0.360962 8.4:-68019790 None:intergenic
AGCTCAAGAATTGATGAGTT+GGG 0.406971 8.4:+68020886 MS.gene79738:CDS
AGCATAAGAAGCACAAAGAC+AGG 0.416670 8.4:+68020038 MS.gene79738:CDS
AGTGCGGTATTAATTTGAAA+TGG 0.421690 8.4:-68019574 None:intergenic
AAAAGGAGATGGCATGCAAT+TGG 0.425102 8.4:+68019682 MS.gene79738:CDS
TCAAGGAGGCTTTCCAACTA+AGG 0.441770 8.4:+68019777 MS.gene79738:CDS
AAAGGAATACCAACAATTGC+AGG 0.443447 8.4:+68019983 MS.gene79738:CDS
TTTCTTTGAGTGATCAGCAC+TGG 0.476818 8.4:-68020167 None:intergenic
TTTCCCTAATAGGCGGAGAA+AGG 0.487140 8.4:+68019965 MS.gene79738:intron
ACAGGGATAAGGACAAGGAC+AGG 0.491569 8.4:+68020056 MS.gene79738:CDS
CACTACCTGCACAGTGTAGT+AGG 0.493509 8.4:+68019644 MS.gene79738:CDS
GAGCTCAAGAATTGATGAGT+TGG 0.493987 8.4:+68020885 MS.gene79738:CDS
GGAGACACAGAAGTAAGAAA+AGG 0.503306 8.4:+68019665 MS.gene79738:CDS
CTCAAAGAAACACCATGAAA+AGG 0.506144 8.4:+68020180 MS.gene79738:CDS
TCCGCGATTGACACAGGAAG+CGG 0.522644 8.4:-68019617 None:intergenic
TCGGATACAGCCTTTCGACT+TGG 0.534768 8.4:+68019748 MS.gene79738:CDS
GAAGCACAAAGACAGGGATA+AGG 0.543181 8.4:+68020045 MS.gene79738:CDS
CAAGGAGGCTTTCCAACTAA+GGG 0.568109 8.4:+68019778 MS.gene79738:CDS
GCTCAAGAATTGATGAGTTG+GGG 0.569389 8.4:+68020887 MS.gene79738:CDS
TCAGGACCGAGGGAGTTGAC+AGG 0.569665 8.4:+68019533 MS.gene79738:intron
TCTTACAGCAGGCAAATCGA+TGG 0.570777 8.4:-68019810 None:intergenic
TTTCGACTTGGATATACTCA+AGG 0.572941 8.4:+68019760 MS.gene79738:CDS
CTTACAGCAGGCAAATCGAT+GGG 0.583764 8.4:-68019809 None:intergenic
TGTGTGTCCGCGATTGACAC+AGG 0.584301 8.4:-68019623 None:intergenic
ACAGCACCTGTCAACTCCCT+CGG 0.592323 8.4:-68019539 None:intergenic
TGTCTCCTACTACACTGTGC+AGG 0.593991 8.4:-68019649 None:intergenic
GCATAAGAAGCACAAAGACA+GGG 0.602938 8.4:+68020039 MS.gene79738:CDS
ACAGAAGTAAGAAAAGGAGA+TGG 0.606393 8.4:+68019671 MS.gene79738:CDS
GAGGGAGTTGACAGGTGCTG+TGG 0.611611 8.4:+68019541 MS.gene79738:CDS
GATTGAATCATGGATCCTGA+AGG 0.613845 8.4:+68019061 None:intergenic
CAAAGACAGGGATAAGGACA+AGG 0.620436 8.4:+68020051 MS.gene79738:CDS
GCCGCTTCCTGTGTCAATCG+CGG 0.625354 8.4:+68019616 MS.gene79738:CDS
CAGGGATAAGGACAAGGACA+GGG 0.626401 8.4:+68020057 MS.gene79738:CDS
CAGAAGCAAAGACAAAGACA+AGG 0.629036 8.4:+68020111 MS.gene79738:CDS
CGACTTGGATATACTCAAGG+AGG 0.629284 8.4:+68019763 MS.gene79738:CDS
TCTCAAGAGAGACAAGTGCT+CGG 0.651092 8.4:+68019729 MS.gene79738:CDS
TTGAGTATATCCAAGTCGAA+AGG 0.652260 8.4:-68019758 None:intergenic
TTTCAGAAGAGGAAGCATGA+CGG 0.652327 8.4:+68020670 MS.gene79738:intron
CTCAAGAATTGATGAGTTGG+GGG 0.664098 8.4:+68020888 MS.gene79738:CDS
GAAGCACAAACATCGTCACA+AGG 0.684990 8.4:+68020087 MS.gene79738:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAAAGATTTTATAGAATT+AGG - chr8.4:68019140-68019159 None:intergenic 10.0%
!!! TTAAATTTTTTGGAATTGAA+TGG + chr8.4:68019335-68019354 MS.gene79738:intron 15.0%
!! TCAATTCCAAAAAATTTAAC+AGG - chr8.4:68019334-68019353 None:intergenic 20.0%
!!! TATTGTGCAAACTTTAAAAA+GGG - chr8.4:68019427-68019446 None:intergenic 20.0%
!!! TTATCACCTGTTAAATTTTT+TGG + chr8.4:68019325-68019344 MS.gene79738:intron 20.0%
!!! TTTGAACAAACATCTATTTT+CGG + chr8.4:68020537-68020556 MS.gene79738:intron 20.0%
! AGATATTGAGCAAAAGTAAA+AGG - chr8.4:68020651-68020670 None:intergenic 25.0%
! CATAATATTCTGTGCTAAAT+AGG - chr8.4:68020473-68020492 None:intergenic 25.0%
! GATATAGTGATGTTAAATAG+CGG + chr8.4:68020348-68020367 MS.gene79738:intron 25.0%
! GTTATCTAATTGACAATACT+TGG + chr8.4:68019230-68019249 MS.gene79738:intron 25.0%
! GTTCTGATTTCATATGTTTA+TGG + chr8.4:68019193-68019212 MS.gene79738:intron 25.0%
! TAATTCTTGCTGAAATCATT+TGG + chr8.4:68019866-68019885 MS.gene79738:intron 25.0%
!! AAATTTCTTCAACCTTTTCA+TGG - chr8.4:68020195-68020214 None:intergenic 25.0%
!! ATTGTGCAAACTTTAAAAAG+GGG - chr8.4:68019426-68019445 None:intergenic 25.0%
!! GTATTGTGCAAACTTTAAAA+AGG - chr8.4:68019428-68019447 None:intergenic 25.0%
ACAAAAAGAAAGACAAAAGC+GGG + chr8.4:68020134-68020153 MS.gene79738:CDS 30.0%
ACACAGAATGTCAAAAAACA+CGG - chr8.4:68019503-68019522 None:intergenic 30.0%
CATTTGCTCTACATACTATT+TGG + chr8.4:68020593-68020612 MS.gene79738:intron 30.0%
GACAAAAAGAAAGACAAAAG+CGG + chr8.4:68020133-68020152 MS.gene79738:CDS 30.0%
GCACAAACATAAAAAGAGTA+AGG + chr8.4:68020714-68020733 MS.gene79738:CDS 30.0%
TCAAGTAAATTTGCAATGAC+AGG - chr8.4:68020269-68020288 None:intergenic 30.0%
TGGACAATGATGTTAAATAC+CGG + chr8.4:68020392-68020411 MS.gene79738:intron 30.0%
TTGCAGAAAAATTCATAGTG+CGG - chr8.4:68019593-68019612 None:intergenic 30.0%
! ACTATGAATTTTTCTGCAAG+AGG + chr8.4:68019594-68019613 MS.gene79738:CDS 30.0%
! AGTGCGGTATTAATTTGAAA+TGG - chr8.4:68019577-68019596 None:intergenic 30.0%
! TAGTTGTTTATTTGCCCTTT+TGG + chr8.4:68019282-68019301 MS.gene79738:intron 30.0%
! TTCCTTGTATTTTCCCTAAT+AGG + chr8.4:68019955-68019974 MS.gene79738:intron 30.0%
!! TTTGATTTTCCTGCAATTGT+TGG - chr8.4:68019995-68020014 None:intergenic 30.0%
AAAGGAATACCAACAATTGC+AGG + chr8.4:68019983-68020002 MS.gene79738:CDS 35.0%
AAAGTTTCAAATCCGCCAAA+AGG - chr8.4:68019300-68019319 None:intergenic 35.0%
AAGTTTCAAATCCGCCAAAA+GGG - chr8.4:68019299-68019318 None:intergenic 35.0%
AATATACACCAGAAATGTGC+TGG - chr8.4:68020795-68020814 None:intergenic 35.0%
ACAGAAGTAAGAAAAGGAGA+TGG + chr8.4:68019671-68019690 MS.gene79738:CDS 35.0%
ACATTGAACAGTGTTGTCAA+TGG - chr8.4:68020570-68020589 None:intergenic 35.0%
AGCTCAAGAATTGATGAGTT+GGG + chr8.4:68020886-68020905 MS.gene79738:CDS 35.0%
ATATACACCAGAAATGTGCT+GGG - chr8.4:68020794-68020813 None:intergenic 35.0%
CTCAAAGAAACACCATGAAA+AGG + chr8.4:68020180-68020199 MS.gene79738:CDS 35.0%
GATCCTGAAGGAAAAAAGTT+TGG + chr8.4:68019073-68019092 MS.gene79738:CDS 35.0%
GCTATAGCGTTATTGCATAA+TGG + chr8.4:68020372-68020391 MS.gene79738:intron 35.0%
TGCTCAATATCTTTCAGAAG+AGG + chr8.4:68020659-68020678 MS.gene79738:intron 35.0%
TTGAGTATATCCAAGTCGAA+AGG - chr8.4:68019761-68019780 None:intergenic 35.0%
TTTAAAAAGGGGTGATTGCA+CGG - chr8.4:68019415-68019434 None:intergenic 35.0%
! CTTGTATTTTCCCTAATAGG+CGG + chr8.4:68019958-68019977 MS.gene79738:intron 35.0%
! TTGTTTATTTGCCCTTTTGG+CGG + chr8.4:68019285-68019304 MS.gene79738:intron 35.0%
!! GTTTGATGATGTGTTCTTTG+TGG + chr8.4:68019916-68019935 MS.gene79738:intron 35.0%
!! TTCTGTGTTTTTGAATCCTC+AGG + chr8.4:68019515-68019534 MS.gene79738:intron 35.0%
!! TTTCGACTTGGATATACTCA+AGG + chr8.4:68019760-68019779 MS.gene79738:CDS 35.0%
AAAAGGAGATGGCATGCAAT+TGG + chr8.4:68019682-68019701 MS.gene79738:CDS 40.0%
ATTCCTTTCTCCGCCTATTA+GGG - chr8.4:68019971-68019990 None:intergenic 40.0%
CAGAAGCAAAGACAAAGACA+AGG + chr8.4:68020111-68020130 MS.gene79738:CDS 40.0%
CCTGAAGGAAAAAAGTTTGG+AGG + chr8.4:68019076-68019095 MS.gene79738:CDS 40.0%
CGCCTATTAGGGAAAATACA+AGG - chr8.4:68019960-68019979 None:intergenic 40.0%
GAAGGAAAAAAGTTTGGAGG+AGG + chr8.4:68019079-68019098 MS.gene79738:CDS 40.0%
GAGCTCAAGAATTGATGAGT+TGG + chr8.4:68020885-68020904 MS.gene79738:CDS 40.0%
GCTACACAATTTCTTACAGC+AGG - chr8.4:68019824-68019843 None:intergenic 40.0%
GCTCAAGAATTGATGAGTTG+GGG + chr8.4:68020887-68020906 MS.gene79738:CDS 40.0%
GGAGACACAGAAGTAAGAAA+AGG + chr8.4:68019665-68019684 MS.gene79738:CDS 40.0%
TATTCCTTTCTCCGCCTATT+AGG - chr8.4:68019972-68019991 None:intergenic 40.0%
TCTATAGCACAATTGCGTAG+TGG + chr8.4:68020512-68020531 MS.gene79738:intron 40.0%
TTGAACAGTGTTGTCAATGG+CGG - chr8.4:68020567-68020586 None:intergenic 40.0%
TTTCTTTGAGTGATCAGCAC+TGG - chr8.4:68020170-68020189 None:intergenic 40.0%
! AGCATAAGAAGCACAAAGAC+AGG + chr8.4:68020038-68020057 MS.gene79738:CDS 40.0%
! CTCAAGAATTGATGAGTTGG+GGG + chr8.4:68020888-68020907 MS.gene79738:CDS 40.0%
! GCATAAGAAGCACAAAGACA+GGG + chr8.4:68020039-68020058 MS.gene79738:CDS 40.0%
! TCTGTGCTAAATAGGTATCG+CGG - chr8.4:68020465-68020484 None:intergenic 40.0%
! TTTCAGAAGAGGAAGCATGA+CGG + chr8.4:68020670-68020689 MS.gene79738:intron 40.0%
!!! CCTCCAAACTTTTTTCCTTC+AGG - chr8.4:68019079-68019098 None:intergenic 40.0%
ATGCAATAACGCTATAGCGC+CGG - chr8.4:68020414-68020433 None:intergenic 45.0%
CAAAGACAGGGATAAGGACA+AGG + chr8.4:68020051-68020070 MS.gene79738:CDS 45.0%
CAAGGAGGCTTTCCAACTAA+GGG + chr8.4:68019778-68019797 MS.gene79738:CDS 45.0%
CTTACAGCAGGCAAATCGAT+GGG - chr8.4:68019812-68019831 None:intergenic 45.0%
GAAGCACAAACATCGTCACA+AGG + chr8.4:68020087-68020106 MS.gene79738:CDS 45.0%
GAAGCACAAAGACAGGGATA+AGG + chr8.4:68020045-68020064 MS.gene79738:CDS 45.0%
TCAAGGAGGCTTTCCAACTA+AGG + chr8.4:68019777-68019796 MS.gene79738:CDS 45.0%
TCTCAAGAGAGACAAGTGCT+CGG + chr8.4:68019729-68019748 MS.gene79738:CDS 45.0%
TCTTACAGCAGGCAAATCGA+TGG - chr8.4:68019813-68019832 None:intergenic 45.0%
TTTCCCTAATAGGCGGAGAA+AGG + chr8.4:68019965-68019984 MS.gene79738:intron 45.0%
TTTCTGTCCCAGCACATTTC+TGG + chr8.4:68020784-68020803 MS.gene79738:intron 45.0%
! CGACTTGGATATACTCAAGG+AGG + chr8.4:68019763-68019782 MS.gene79738:CDS 45.0%
! TGATGAGTTGGGGGCAATAA+AGG + chr8.4:68020897-68020916 MS.gene79738:CDS 45.0%
! TTTTGAATCCTCAGGACCGA+GGG + chr8.4:68019523-68019542 MS.gene79738:intron 45.0%
! TTTTTGAATCCTCAGGACCG+AGG + chr8.4:68019522-68019541 MS.gene79738:intron 45.0%
ACAGGGATAAGGACAAGGAC+AGG + chr8.4:68020056-68020075 MS.gene79738:CDS 50.0%
CACTACCTGCACAGTGTAGT+AGG + chr8.4:68019644-68019663 MS.gene79738:CDS 50.0%
CAGGGATAAGGACAAGGACA+GGG + chr8.4:68020057-68020076 MS.gene79738:CDS 50.0%
TCGGATACAGCCTTTCGACT+TGG + chr8.4:68019748-68019767 MS.gene79738:CDS 50.0%
TGTCTCCTACTACACTGTGC+AGG - chr8.4:68019652-68019671 None:intergenic 50.0%
TTGGGGGCAATAAAGGTTGC+TGG + chr8.4:68020904-68020923 MS.gene79738:CDS 50.0%
! TGGGAGCTGTTTCCCTTAGT+TGG - chr8.4:68019793-68019812 None:intergenic 50.0%
ACAGCACCTGTCAACTCCCT+CGG - chr8.4:68019542-68019561 None:intergenic 55.0%
TCCGCGATTGACACAGGAAG+CGG - chr8.4:68019620-68019639 None:intergenic 55.0%
TGTGTGTCCGCGATTGACAC+AGG - chr8.4:68019626-68019645 None:intergenic 55.0%
GCCGCTTCCTGTGTCAATCG+CGG + chr8.4:68019616-68019635 MS.gene79738:CDS 60.0%
TCAGGACCGAGGGAGTTGAC+AGG + chr8.4:68019533-68019552 MS.gene79738:intron 60.0%
TGTCAACTCCCTCGGTCCTG+AGG - chr8.4:68019534-68019553 None:intergenic 60.0%
! GAGGGAGTTGACAGGTGCTG+TGG + chr8.4:68019541-68019560 MS.gene79738:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 68019070 68020930 68019070 ID=MS.gene79738
chr8.4 mRNA 68019070 68020930 68019070 ID=MS.gene79738.t1;Parent=MS.gene79738
chr8.4 exon 68019070 68019100 68019070 ID=MS.gene79738.t1.exon1;Parent=MS.gene79738.t1
chr8.4 CDS 68019070 68019100 68019070 ID=cds.MS.gene79738.t1;Parent=MS.gene79738.t1
chr8.4 exon 68019537 68019826 68019537 ID=MS.gene79738.t1.exon2;Parent=MS.gene79738.t1
chr8.4 CDS 68019537 68019826 68019537 ID=cds.MS.gene79738.t1;Parent=MS.gene79738.t1
chr8.4 exon 68019977 68020201 68019977 ID=MS.gene79738.t1.exon3;Parent=MS.gene79738.t1
chr8.4 CDS 68019977 68020201 68019977 ID=cds.MS.gene79738.t1;Parent=MS.gene79738.t1
chr8.4 exon 68020676 68020735 68020676 ID=MS.gene79738.t1.exon4;Parent=MS.gene79738.t1
chr8.4 CDS 68020676 68020735 68020676 ID=cds.MS.gene79738.t1;Parent=MS.gene79738.t1
chr8.4 exon 68020880 68020930 68020880 ID=MS.gene79738.t1.exon5;Parent=MS.gene79738.t1
chr8.4 CDS 68020880 68020930 68020880 ID=cds.MS.gene79738.t1;Parent=MS.gene79738.t1
Gene Sequence

>MS.gene79738

ATGGATCCTGAAGGAAAAAAGTTTGGAGGAGGTAAAAGTTTGTTATGATAGTGCTTTATGTTGTATACCTAATTCTATAAAATCTTTATTAGATGTCTCTGTTTTGTATTATTGTATATACAAGTTCTGATTTCATATGTTTATGGCACTGACTGTGCTTGTTATCTAATTGACAATACTTGGTTAAATTTTTAATCAGTTATTCGTGATCTTAGTTGTTTATTTGCCCTTTTGGCGGATTTGAAACTTTGTCAATTATCACCTGTTAAATTTTTTGGAATTGAATGGAAAAGATTAAATTCTCTTAGCATCTTTTGAAGATTGAGTCATAGATAAATATTACCGTGCAATCACCCCTTTTTAAAGTTTGCACAATACATTTTCTTGTGTATATTTTATATTTAGTTAACACTTACTCATCAAAGAAAAACCGTGTTTTTTGACATTCTGTGTTTTTGAATCCTCAGGACCGAGGGAGTTGACAGGTGCTGTGGATCTCATAAGCCATTTCAAATTAATACCGCACTATGAATTTTTCTGCAAGAGGCCGCTTCCTGTGTCAATCGCGGACACACACTACCTGCACAGTGTAGTAGGAGACACAGAAGTAAGAAAAGGAGATGGCATGCAATTGGACGAACTCATTCAGAATACATCTTTCTCAAGAGAGACAAGTGCTCGGATACAGCCTTTCGACTTGGATATACTCAAGGAGGCTTTCCAACTAAGGGAAACAGCTCCCATCGATTTGCCTGCTGTAAGAAATTGTGTAGCGATTTTGATCATACTTAGTTTTTAATTCTTGCTGAAATCATTTGGCATGCTATCTTAGTATTTTTTAACAAAGTTTGATGATGTGTTCTTTGTGGACTTATATCTTGATTGTTCCTTGTATTTTCCCTAATAGGCGGAGAAAGGAATACCAACAATTGCAGGAAAATCAAAAAGTGAGAAAGACAAAGAGAAGAAGCATAAGAAGCACAAAGACAGGGATAAGGACAAGGACAGGGAGCATAAGAAGCACAAACATCGTCACAAGGACAGAAGCAAAGACAAAGACAAGGACAAAAAGAAAGACAAAAGCGGGCATCGTGATTCCAGTGCTGATCACTCAAAGAAACACCATGAAAAGGTTGAAGAAATTTAGTTTGCTGCATCTGTATTCTTCAGATTGAGAGAAAATATGTTCTGAAAATCCTGTCATTGCAAATTTACTTGACTGTTTTTTATGATTTTTAATTAGTGTCATGCTGATACTGTTAACTCTCATTTTAGTTTGATATAGTGATGTTAAATAGCGGTGCTATAGCGTTATTGCATAATGGACAATGATGTTAAATACCGGCGCTATAGCGTTATTGCATAATGGAACTTGAACAAATCACTATTGTTCCGCGATACCTATTTAGCACAGAATATTATGAAATAACAGTTATAGCAGCTCTATAGCACAATTGCGTAGTGGAATTTGAACAAACATCTATTTTCGGCGATCCGCCATTGACAACACTGTTCAATGTCAACATTTGCTCTACATACTATTTGGACATGTTACAGATATTTAGTTCTATTTATTAACCTTTTACTTTTGCTCAATATCTTTCAGAAGAGGAAGCATGACGGAGATGATGATGCTAATGATGTGCACAAACATAAAAAGAGTAAGGTAATAAAGTAGTAAGAAGCTTAGTCTTGTGATGAGTTTTTTCTGTCTTTTCTGTCCCAGCACATTTCTGGTGTATATTTCTTAGTGATTGACAGTGTCTAATCTTCAATGAACTTCTGTGCTCAATTTCTTAATTTTTTGCAGCACAAGAGCTCAAGAATTGATGAGTTGGGGGCAATAAAGGTTGCTGGCTGA

Protein sequence

>MS.gene79738.t1

MDPEGKKFGGGPRELTGAVDLISHFKLIPHYEFFCKRPLPVSIADTHYLHSVVGDTEVRKGDGMQLDELIQNTSFSRETSARIQPFDLDILKEAFQLRETAPIDLPAAEKGIPTIAGKSKSEKDKEKKHKKHKDRDKDKDREHKKHKHRHKDRSKDKDKDKKKDKSGHRDSSADHSKKHHEKKRKHDGDDDANDVHKHKKSKHKSSRIDELGAIKVAG