Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80269.t1 | XP_003613226.1 | 93.6 | 251 | 12 | 2 | 1 | 250 | 1 | 248 | 4.70E-104 | 387.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80269.t1 | G7K1D1 | 93.6 | 251 | 12 | 2 | 1 | 250 | 1 | 248 | 3.4e-104 | 387.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049861 | MS.gene80269 | 0.834137 | 3.57E-56 | -1.69E-46 |
| MS.gene04998 | MS.gene80269 | 0.876449 | 1.38E-68 | -1.69E-46 |
| MS.gene051184 | MS.gene80269 | 0.82075 | 5.82E-53 | -1.69E-46 |
| MS.gene051903 | MS.gene80269 | 0.802303 | 5.98E-49 | -1.69E-46 |
| MS.gene052296 | MS.gene80269 | 0.817309 | 3.53E-52 | -1.69E-46 |
| MS.gene052818 | MS.gene80269 | -0.825055 | 5.77E-54 | -1.69E-46 |
| MS.gene052878 | MS.gene80269 | 0.801026 | 1.09E-48 | -1.69E-46 |
| MS.gene053298 | MS.gene80269 | 0.83196 | 1.24E-55 | -1.69E-46 |
| MS.gene053879 | MS.gene80269 | 0.80858 | 2.89E-50 | -1.69E-46 |
| MS.gene05469 | MS.gene80269 | 0.800537 | 1.38E-48 | -1.69E-46 |
| MS.gene056752 | MS.gene80269 | 0.801369 | 9.30E-49 | -1.69E-46 |
| MS.gene056992 | MS.gene80269 | 0.82315 | 1.62E-53 | -1.69E-46 |
| MS.gene057999 | MS.gene80269 | 0.865168 | 7.18E-65 | -1.69E-46 |
| MS.gene058074 | MS.gene80269 | 0.83674 | 7.84E-57 | -1.69E-46 |
| MS.gene06011 | MS.gene80269 | 0.8052 | 1.50E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80269.t1 | MTR_5g034230 | 92.430 | 251 | 15 | 2 | 1 | 250 | 1 | 248 | 1.01e-135 | 382 |
| MS.gene80269.t1 | MTR_8g064650 | 38.197 | 233 | 126 | 7 | 30 | 250 | 94 | 320 | 1.05e-37 | 134 |
| MS.gene80269.t1 | MTR_3g077890 | 31.429 | 245 | 111 | 10 | 34 | 250 | 70 | 285 | 1.48e-21 | 91.3 |
| MS.gene80269.t1 | MTR_4g105750 | 33.333 | 168 | 87 | 8 | 25 | 177 | 27 | 184 | 1.32e-14 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80269.t1 | AT1G70420 | 41.762 | 261 | 126 | 7 | 9 | 250 | 19 | 272 | 2.07e-50 | 166 |
| MS.gene80269.t1 | AT1G23710 | 36.770 | 291 | 137 | 9 | 1 | 250 | 11 | 295 | 1.38e-40 | 141 |
| MS.gene80269.t1 | AT5G62770 | 27.236 | 246 | 109 | 10 | 47 | 250 | 51 | 268 | 1.43e-14 | 71.6 |
| MS.gene80269.t1 | AT3G27880 | 35.606 | 132 | 58 | 3 | 128 | 242 | 115 | 236 | 5.35e-13 | 67.0 |
Find 44 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACCTCTTAGCGAGGTTTC+TGG | 0.160838 | 5.4:+24205509 | MS.gene80269:CDS |
| CCAAACAATTCTTGCCTATA+TGG | 0.241815 | 5.4:-24206166 | None:intergenic |
| CCATATAGGCAAGAATTGTT+TGG | 0.312992 | 5.4:+24206166 | MS.gene80269:CDS |
| GGAAATCTTATTTGCCATAT+AGG | 0.323222 | 5.4:+24206152 | MS.gene80269:CDS |
| GAAAATGGTGAAAGGGAAAA+AGG | 0.342563 | 5.4:+24206060 | MS.gene80269:CDS |
| CATATCGAAGATTCTCAATA+AGG | 0.355294 | 5.4:-24206235 | None:intergenic |
| CATATAGGCAAGAATTGTTT+GGG | 0.361904 | 5.4:+24206167 | MS.gene80269:CDS |
| GACGATTACGATAACGAATC+TGG | 0.373750 | 5.4:+24205764 | MS.gene80269:CDS |
| GCGGAAATCGGTGAACCTTT+AGG | 0.386454 | 5.4:-24205671 | None:intergenic |
| CTTTGCTAGTACAAATGGAT+TGG | 0.388215 | 5.4:+24206192 | MS.gene80269:CDS |
| AACACATCGTCAGCGGAAAT+CGG | 0.392748 | 5.4:-24205683 | None:intergenic |
| GGGTTCTTTGCTAGTACAAA+TGG | 0.406783 | 5.4:+24206187 | MS.gene80269:CDS |
| TGTTGTAAAGAAAGTGAAAA+TGG | 0.410247 | 5.4:+24206045 | MS.gene80269:CDS |
| TTTGCTAGTACAAATGGATT+GGG | 0.425121 | 5.4:+24206193 | MS.gene80269:CDS |
| AGCCACGAGTTCTGCGGCGG+TGG | 0.451550 | 5.4:+24205841 | MS.gene80269:CDS |
| AAAGGCGGCTCCGGCGCCGT+CGG | 0.456704 | 5.4:+24206078 | MS.gene80269:CDS |
| TTCTCATGCGCCGACGGCGC+CGG | 0.469285 | 5.4:-24206088 | None:intergenic |
| ACGAGTTCTGCGGCGGTGGA+AGG | 0.479410 | 5.4:+24205845 | MS.gene80269:CDS |
| TTTCCAGAAACCTCGCTAAG+AGG | 0.489550 | 5.4:-24205512 | None:intergenic |
| TGTGATGAGTAAAGCGAGGA+AGG | 0.493698 | 5.4:+24206117 | MS.gene80269:CDS |
| CGAGGAAGGAAAGTGATAAA+CGG | 0.500982 | 5.4:+24206131 | MS.gene80269:CDS |
| AGAAAGTGAAAATGGTGAAA+GGG | 0.503176 | 5.4:+24206053 | MS.gene80269:CDS |
| TCACTGTTACTTCTCAGCTT+CGG | 0.506640 | 5.4:-24205947 | None:intergenic |
| GCGGTGAAGAGTAATTCAAC+AGG | 0.510549 | 5.4:+24205896 | MS.gene80269:CDS |
| CGAGTTCTGCGGCGGTGGAA+GGG | 0.519054 | 5.4:+24205846 | MS.gene80269:CDS |
| CAACAGGATCATCAAAGCTA+TGG | 0.521111 | 5.4:+24205912 | MS.gene80269:CDS |
| AAGAAAGTGAAAATGGTGAA+AGG | 0.523662 | 5.4:+24206052 | MS.gene80269:CDS |
| TTCCACCGCCGCAGAACTCG+TGG | 0.531134 | 5.4:-24205843 | None:intergenic |
| GCTGACGATGTGTTTGATAA+CGG | 0.541968 | 5.4:+24205692 | MS.gene80269:CDS |
| CGAATCTGGAAACCGTTCGC+CGG | 0.550648 | 5.4:+24205778 | MS.gene80269:CDS |
| AAAATTTCTTTACCGGCGAA+CGG | 0.570175 | 5.4:-24205790 | None:intergenic |
| TGGAAGGGACGTACTGCGAG+TGG | 0.573288 | 5.4:+24205861 | MS.gene80269:CDS |
| TTCAACTTCACCTCTTAGCG+AGG | 0.585504 | 5.4:+24205502 | MS.gene80269:CDS |
| TAATGTTTCTCATGCGCCGA+CGG | 0.590741 | 5.4:-24206094 | None:intergenic |
| GTGGCTGTCTTCGACGAAGA+CGG | 0.621629 | 5.4:-24205824 | None:intergenic |
| CAAATGGATTGGGAATGAGT+AGG | 0.630616 | 5.4:+24206203 | MS.gene80269:CDS |
| AGATTCGTTATCGTAATCGT+CGG | 0.637566 | 5.4:-24205762 | None:intergenic |
| GTTATCAAACACATCGTCAG+CGG | 0.641120 | 5.4:-24205690 | None:intergenic |
| GACAGCCACGAGTTCTGCGG+CGG | 0.647643 | 5.4:+24205838 | MS.gene80269:CDS |
| GAAGACAGCCACGAGTTCTG+CGG | 0.653644 | 5.4:+24205835 | MS.gene80269:CDS |
| CGAGTGGAAGCCAACAAAAG+CGG | 0.658976 | 5.4:+24205877 | MS.gene80269:CDS |
| CGAAAACGAAGATAAACACA+AGG | 0.679532 | 5.4:+24205589 | MS.gene80269:CDS |
| ATTATGTGATGAGTAAAGCG+AGG | 0.682546 | 5.4:+24206113 | MS.gene80269:CDS |
| AAGCTGAGAAGTAACAGTGA+CGG | 0.695156 | 5.4:+24205950 | MS.gene80269:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGTTGTAAAGAAAGTGAAAA+TGG | + | chr5.4:24206045-24206064 | MS.gene80269:CDS | 25.0% |
| AAGAAAGTGAAAATGGTGAA+AGG | + | chr5.4:24206052-24206071 | MS.gene80269:CDS | 30.0% | |
| AGAAAGTGAAAATGGTGAAA+GGG | + | chr5.4:24206053-24206072 | MS.gene80269:CDS | 30.0% | |
| CATATAGGCAAGAATTGTTT+GGG | + | chr5.4:24206167-24206186 | MS.gene80269:CDS | 30.0% | |
| TCGACGAAAAATTTCTTTAC+CGG | - | chr5.4:24205800-24205819 | None:intergenic | 30.0% | |
| TGACCGAAAAAAGGAAAAAT+CGG | - | chr5.4:24205728-24205747 | None:intergenic | 30.0% | |
| ! | GGAAATCTTATTTGCCATAT+AGG | + | chr5.4:24206152-24206171 | MS.gene80269:CDS | 30.0% |
| ! | TTTGCTAGTACAAATGGATT+GGG | + | chr5.4:24206193-24206212 | MS.gene80269:CDS | 30.0% |
| AAAATTTCTTTACCGGCGAA+CGG | - | chr5.4:24205793-24205812 | None:intergenic | 35.0% | |
| AGATTCGTTATCGTAATCGT+CGG | - | chr5.4:24205765-24205784 | None:intergenic | 35.0% | |
| ATTATGTGATGAGTAAAGCG+AGG | + | chr5.4:24206113-24206132 | MS.gene80269:CDS | 35.0% | |
| CCAAACAATTCTTGCCTATA+TGG | - | chr5.4:24206169-24206188 | None:intergenic | 35.0% | |
| CCATATAGGCAAGAATTGTT+TGG | + | chr5.4:24206166-24206185 | MS.gene80269:CDS | 35.0% | |
| CGAAAACGAAGATAAACACA+AGG | + | chr5.4:24205589-24205608 | MS.gene80269:CDS | 35.0% | |
| CTTTGCTAGTACAAATGGAT+TGG | + | chr5.4:24206192-24206211 | MS.gene80269:CDS | 35.0% | |
| GAAAATGGTGAAAGGGAAAA+AGG | + | chr5.4:24206060-24206079 | MS.gene80269:CDS | 35.0% | |
| ! | CGTCCGATTTTTCCTTTTTT+CGG | + | chr5.4:24205722-24205741 | MS.gene80269:CDS | 35.0% |
| !! | TTTGTTTTCGCAGATCCTAA+AGG | + | chr5.4:24205656-24205675 | MS.gene80269:CDS | 35.0% |
| !!! | GATTTTTCCTTTTTTCGGTC+AGG | + | chr5.4:24205727-24205746 | MS.gene80269:CDS | 35.0% |
| AAGCTGAGAAGTAACAGTGA+CGG | + | chr5.4:24205950-24205969 | MS.gene80269:CDS | 40.0% | |
| AATGGTGAAAGGGAAAAAGG+CGG | + | chr5.4:24206063-24206082 | MS.gene80269:CDS | 40.0% | |
| CAACAGGATCATCAAAGCTA+TGG | + | chr5.4:24205912-24205931 | MS.gene80269:CDS | 40.0% | |
| CGAGGAAGGAAAGTGATAAA+CGG | + | chr5.4:24206131-24206150 | MS.gene80269:CDS | 40.0% | |
| GACGATTACGATAACGAATC+TGG | + | chr5.4:24205764-24205783 | MS.gene80269:CDS | 40.0% | |
| GCTGACGATGTGTTTGATAA+CGG | + | chr5.4:24205692-24205711 | MS.gene80269:CDS | 40.0% | |
| GGGTTCTTTGCTAGTACAAA+TGG | + | chr5.4:24206187-24206206 | MS.gene80269:CDS | 40.0% | |
| GTTATCAAACACATCGTCAG+CGG | - | chr5.4:24205693-24205712 | None:intergenic | 40.0% | |
| TGAAGATCCTGACCGAAAAA+AGG | - | chr5.4:24205737-24205756 | None:intergenic | 40.0% | |
| ! | TACAACATTTTTCGTCTCGC+TGG | - | chr5.4:24206032-24206051 | None:intergenic | 40.0% |
| ! | TCACTGTTACTTCTCAGCTT+CGG | - | chr5.4:24205950-24205969 | None:intergenic | 40.0% |
| !! | CAAATGGATTGGGAATGAGT+AGG | + | chr5.4:24206203-24206222 | MS.gene80269:CDS | 40.0% |
| !! | TTACTCTTCACCGCTTTTGT+TGG | - | chr5.4:24205890-24205909 | None:intergenic | 40.0% |
| AACACATCGTCAGCGGAAAT+CGG | - | chr5.4:24205686-24205705 | None:intergenic | 45.0% | |
| GCGGTGAAGAGTAATTCAAC+AGG | + | chr5.4:24205896-24205915 | MS.gene80269:CDS | 45.0% | |
| TAATGTTTCTCATGCGCCGA+CGG | - | chr5.4:24206097-24206116 | None:intergenic | 45.0% | |
| TGTGATGAGTAAAGCGAGGA+AGG | + | chr5.4:24206117-24206136 | MS.gene80269:CDS | 45.0% | |
| TTCAACTTCACCTCTTAGCG+AGG | + | chr5.4:24205502-24205521 | MS.gene80269:CDS | 45.0% | |
| TTTCCAGAAACCTCGCTAAG+AGG | - | chr5.4:24205515-24205534 | None:intergenic | 45.0% | |
| !! | CGCGTTCGTGTTTTTGAATC+CGG | + | chr5.4:24205979-24205998 | MS.gene80269:CDS | 45.0% |
| CGAGTGGAAGCCAACAAAAG+CGG | + | chr5.4:24205877-24205896 | MS.gene80269:CDS | 50.0% | |
| GCGGAAATCGGTGAACCTTT+AGG | - | chr5.4:24205674-24205693 | None:intergenic | 50.0% | |
| TCACCTCTTAGCGAGGTTTC+TGG | + | chr5.4:24205509-24205528 | MS.gene80269:CDS | 50.0% | |
| ! | ATTTTTCGTCTCGCTGGCGA+CGG | - | chr5.4:24206026-24206045 | None:intergenic | 50.0% |
| ! | CTCGTTTTCTCTACCTTCGC+CGG | - | chr5.4:24206001-24206020 | None:intergenic | 50.0% |
| !! | CGTGTTTTTGAATCCGGCGA+AGG | + | chr5.4:24205985-24206004 | MS.gene80269:CDS | 50.0% |
| CGAATCTGGAAACCGTTCGC+CGG | + | chr5.4:24205778-24205797 | MS.gene80269:CDS | 55.0% | |
| GAAAGGGAAAAAGGCGGCTC+CGG | + | chr5.4:24206069-24206088 | MS.gene80269:CDS | 55.0% | |
| GAAGACAGCCACGAGTTCTG+CGG | + | chr5.4:24205835-24205854 | MS.gene80269:CDS | 55.0% | |
| GTGGCTGTCTTCGACGAAGA+CGG | - | chr5.4:24205827-24205846 | None:intergenic | 55.0% | |
| TGGAAGGGACGTACTGCGAG+TGG | + | chr5.4:24205861-24205880 | MS.gene80269:CDS | 60.0% | |
| ACGAGTTCTGCGGCGGTGGA+AGG | + | chr5.4:24205845-24205864 | MS.gene80269:CDS | 65.0% | |
| CGAGTTCTGCGGCGGTGGAA+GGG | + | chr5.4:24205846-24205865 | MS.gene80269:CDS | 65.0% | |
| GACAGCCACGAGTTCTGCGG+CGG | + | chr5.4:24205838-24205857 | MS.gene80269:CDS | 65.0% | |
| TTCCACCGCCGCAGAACTCG+TGG | - | chr5.4:24205846-24205865 | None:intergenic | 65.0% | |
| AGCCACGAGTTCTGCGGCGG+TGG | + | chr5.4:24205841-24205860 | MS.gene80269:CDS | 70.0% | |
| ! | TTCTCATGCGCCGACGGCGC+CGG | - | chr5.4:24206091-24206110 | None:intergenic | 70.0% |
| AAAGGCGGCTCCGGCGCCGT+CGG | + | chr5.4:24206078-24206097 | MS.gene80269:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 24205491 | 24206243 | 24205491 | ID=MS.gene80269 |
| chr5.4 | mRNA | 24205491 | 24206243 | 24205491 | ID=MS.gene80269.t1;Parent=MS.gene80269 |
| chr5.4 | exon | 24205491 | 24206243 | 24205491 | ID=MS.gene80269.t1.exon1;Parent=MS.gene80269.t1 |
| chr5.4 | CDS | 24205491 | 24206243 | 24205491 | ID=cds.MS.gene80269.t1;Parent=MS.gene80269.t1 |
| Gene Sequence |
| Protein sequence |
