Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81946.t1 | XP_013452197.1 | 87.5 | 168 | 9 | 1 | 1 | 168 | 2 | 157 | 3.40E-74 | 287.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81946.t1 | Q6PBM8 | 36.3 | 157 | 74 | 5 | 4 | 154 | 17 | 153 | 2.0e-14 | 80.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81946.t1 | G7JDR6 | 87.5 | 168 | 9 | 1 | 1 | 168 | 2 | 157 | 2.5e-74 | 287.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050288 | MS.gene81946 | 0.80043 | 1.45E-48 | -1.69E-46 |
| MS.gene050320 | MS.gene81946 | 0.807876 | 4.08E-50 | -1.69E-46 |
| MS.gene051245 | MS.gene81946 | 0.815825 | 7.58E-52 | -1.69E-46 |
| MS.gene052309 | MS.gene81946 | -0.814149 | 1.78E-51 | -1.69E-46 |
| MS.gene053109 | MS.gene81946 | 0.803423 | 3.51E-49 | -1.69E-46 |
| MS.gene057079 | MS.gene81946 | 0.827807 | 1.28E-54 | -1.69E-46 |
| MS.gene057234 | MS.gene81946 | 0.804319 | 2.29E-49 | -1.69E-46 |
| MS.gene057452 | MS.gene81946 | -0.807786 | 4.26E-50 | -1.69E-46 |
| MS.gene057999 | MS.gene81946 | 0.824085 | 9.77E-54 | -1.69E-46 |
| MS.gene058238 | MS.gene81946 | 0.831653 | 1.48E-55 | -1.69E-46 |
| MS.gene059830 | MS.gene81946 | 0.800643 | 1.31E-48 | -1.69E-46 |
| MS.gene060297 | MS.gene81946 | 0.864165 | 1.48E-64 | -1.69E-46 |
| MS.gene060586 | MS.gene81946 | 0.812636 | 3.84E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81946.t1 | MTR_6g048060 | 87.500 | 168 | 9 | 1 | 1 | 168 | 2 | 157 | 2.08e-106 | 301 |
| MS.gene81946.t1 | MTR_0022s0360 | 86.905 | 168 | 10 | 1 | 1 | 168 | 2 | 157 | 1.60e-104 | 296 |
| MS.gene81946.t1 | MTR_6g048060 | 85.517 | 145 | 9 | 1 | 24 | 168 | 38 | 170 | 5.39e-87 | 252 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81946.t1 | AT5G52545 | 60.843 | 166 | 50 | 4 | 1 | 163 | 2 | 155 | 9.92e-65 | 195 |
| MS.gene81946.t1 | AT5G52545 | 50.633 | 79 | 36 | 3 | 88 | 163 | 1 | 79 | 1.69e-17 | 73.2 |
Find 0 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GCTTATTTTTTATATATTTT+TGG | - | 43366:323537-323556 | MS.gene81946:CDS | 10.0% |
| ! | AAAAACAGCACTTAATACAT+TGG | + | 43366:323646-323665 | None:intergenic | 25.0% |
| ! | AAGAATATGCATATAGTACA+AGG | + | 43366:323972-323991 | None:intergenic | 25.0% |
| ! | ATAAAAAATAAGCCATGTTG+AGG | + | 43366:323530-323549 | None:intergenic | 25.0% |
| AACGCTAATATTACTTGTGA+AGG | - | 43366:323562-323581 | MS.gene81946:CDS | 30.0% | |
| ACCTATATTTGAATCTCTCT+TGG | + | 43366:323763-323782 | None:intergenic | 30.0% | |
| ATAACTATTGCCATGACAAA+CGG | - | 43366:323816-323835 | MS.gene81946:intron | 30.0% | |
| GAACAAGTTAAAGTCTTTAG+AGG | - | 43366:324461-324480 | MS.gene81946:CDS | 30.0% | |
| TCGATTTCTTAAAGCATGTT+TGG | - | 43366:323687-323706 | MS.gene81946:CDS | 30.0% | |
| TTATCATCAACAGAACCATT+AGG | + | 43366:323443-323462 | None:intergenic | 30.0% | |
| ! | AAGTGTGCATTGAAAAGTAT+AGG | - | 43366:323608-323627 | MS.gene81946:CDS | 30.0% |
| ! | CTCAAAATGTTTTCTCCTTA+TGG | - | 43366:324033-324052 | MS.gene81946:intron | 30.0% |
| !! | ACCTCATTTATTTCTATGGA+CGG | + | 43366:324171-324190 | None:intergenic | 30.0% |
| !! | ACGTACCTCATTTATTTCTA+TGG | + | 43366:324175-324194 | None:intergenic | 30.0% |
| AAGTTTGGATTTCTGATCTG+AGG | + | 43366:323793-323812 | None:intergenic | 35.0% | |
| CAATCTTAAAGTGAGAATGC+AGG | - | 43366:324242-324261 | MS.gene81946:intron | 35.0% | |
| CAATTGCATCATGATATGAC+TGG | - | 43366:323876-323895 | MS.gene81946:CDS | 35.0% | |
| CTACAAAAGCAGTAAGTGAA+GGG | - | 43366:324370-324389 | MS.gene81946:intron | 35.0% | |
| GCCAAGAGAGATTCAAATAT+AGG | - | 43366:323759-323778 | MS.gene81946:intron | 35.0% | |
| GGCAATAGTTATTGCAAGTT+TGG | + | 43366:323808-323827 | None:intergenic | 35.0% | |
| TCCGTCCATAGAAATAAATG+AGG | - | 43366:324167-324186 | MS.gene81946:intron | 35.0% | |
| TCTACAAAAGCAGTAAGTGA+AGG | - | 43366:324369-324388 | MS.gene81946:intron | 35.0% | |
| TGTGAAAATAGACTCTACGT+TGG | - | 43366:323464-323483 | MS.gene81946:CDS | 35.0% | |
| ! | CAAGTTAAAGTCTTTAGAGG+AGG | - | 43366:324464-324483 | MS.gene81946:CDS | 35.0% |
| ! | CATCAGAAACCTAGATTTTG+TGG | - | 43366:323925-323944 | MS.gene81946:CDS | 35.0% |
| ! | GCTTGTTAAGGAATCAAAGA+TGG | - | 43366:323584-323603 | MS.gene81946:CDS | 35.0% |
| !! | CTGCATTCTCACTTTAAGAT+TGG | + | 43366:324244-324263 | None:intergenic | 35.0% |
| !! | TTACTTGTGAAGGCTTGTTA+AGG | - | 43366:323572-323591 | MS.gene81946:CDS | 35.0% |
| AAGTTACATATCACTGGTGG+TGG | - | 43366:324396-324415 | MS.gene81946:intron | 40.0% | |
| ACAAGGAAAAGAAACCGAAC+AGG | + | 43366:323955-323974 | None:intergenic | 40.0% | |
| CACAAGTTACATATCACTGG+TGG | - | 43366:324393-324412 | MS.gene81946:intron | 40.0% | |
| TGTATTCGTGACCATGATTG+AGG | + | 43366:323907-323926 | None:intergenic | 40.0% | |
| ! | AATGATGGAGACAGAGAAAG+AGG | + | 43366:323997-324016 | None:intergenic | 40.0% |
| ! | GTCACTCAACTTCTGTTTCT+TGG | + | 43366:324502-324521 | None:intergenic | 40.0% |
| ACAGGTGTGCCACAAAATCT+AGG | + | 43366:323937-323956 | None:intergenic | 45.0% | |
| CATCCAGTACAGCACAAAAG+AGG | - | 43366:324128-324147 | MS.gene81946:intron | 45.0% | |
| GAGAATGCAGGAAGCAGTAT+TGG | - | 43366:324254-324273 | MS.gene81946:intron | 45.0% | |
| GGGCACAAGTTACATATCAC+TGG | - | 43366:324390-324409 | MS.gene81946:intron | 45.0% | |
| TCGGACACGATCTTTCCATA+AGG | + | 43366:324051-324070 | None:intergenic | 45.0% | |
| TTATGGAAAGATCGTGTCCG+AGG | - | 43366:324050-324069 | MS.gene81946:intron | 45.0% | |
| TTGGCCAAGGAGAAAATGCA+CGG | - | 43366:324273-324292 | MS.gene81946:intron | 45.0% | |
| ! | AGAGCTATCTGCCTTTTCCA+GGG | + | 43366:324352-324371 | None:intergenic | 45.0% |
| ! | GATTTTGTGGCACACCTGTT+CGG | - | 43366:323938-323957 | MS.gene81946:intron | 45.0% |
| ! | GTACCTCTTTTGTGCTGTAC+TGG | + | 43366:324134-324153 | None:intergenic | 45.0% |
| ! | TAGAGCTATCTGCCTTTTCC+AGG | + | 43366:324353-324372 | None:intergenic | 45.0% |
| !! | AGAGCAGCTCTGGAAAATGA+TGG | + | 43366:324012-324031 | None:intergenic | 45.0% |
| ATTCCTGCGACCGTTTGTCA+TGG | + | 43366:323829-323848 | None:intergenic | 50.0% | |
| CAAGACGAACAACCAATGGC+CGG | + | 43366:324314-324333 | None:intergenic | 50.0% | |
| CCAAGGAGAAAATGCACGGA+AGG | - | 43366:324277-324296 | MS.gene81946:intron | 50.0% | |
| TGATAGGTACGCCCTGGAAA+AGG | - | 43366:324338-324357 | MS.gene81946:intron | 50.0% | |
| TGTGTGCCACAAGAAGTCCT+CGG | + | 43366:324070-324089 | None:intergenic | 50.0% | |
| TTGTTCGTCTTGCGGGTGAT+AGG | - | 43366:324322-324341 | MS.gene81946:intron | 50.0% | |
| ! | CATTTTGAGCAGAGCAGCTC+TGG | + | 43366:324022-324041 | None:intergenic | 50.0% |
| ! | CCATTGGTTGTTCGTCTTGC+GGG | - | 43366:324315-324334 | MS.gene81946:intron | 50.0% |
| ! | CCTTCCGTGCATTTTCTCCT+TGG | + | 43366:324280-324299 | None:intergenic | 50.0% |
| ! | GCCATTGGTTGTTCGTCTTG+CGG | - | 43366:324314-324333 | MS.gene81946:intron | 50.0% |
| !! | ACATATCACTGGTGGTGGCA+TGG | - | 43366:324401-324420 | MS.gene81946:intron | 50.0% |
| !! | CATATCACTGGTGGTGGCAT+GGG | - | 43366:324402-324421 | MS.gene81946:intron | 50.0% |
| !! | GACTGGTTCGACCTCAATCA+TGG | - | 43366:323893-323912 | MS.gene81946:CDS | 50.0% |
| ACACACGTGGCCCAAAACGT+GGG | - | 43366:324085-324104 | MS.gene81946:intron | 55.0% | |
| CCCGCAAGACGAACAACCAA+TGG | + | 43366:324318-324337 | None:intergenic | 55.0% | |
| GACTTCTTGTGGCACACACG+TGG | - | 43366:324072-324091 | MS.gene81946:intron | 55.0% | |
| TCGTGTCCGAGGACTTCTTG+TGG | - | 43366:324061-324080 | MS.gene81946:intron | 55.0% | |
| TGGATGAAAGCGAAACCGCG+TGG | + | 43366:324114-324133 | None:intergenic | 55.0% | |
| TTGCCATGACAAACGGTCGC+AGG | - | 43366:323823-323842 | MS.gene81946:CDS | 55.0% | |
| !! | ACGGAAGGTTAGCTTGCAGC+CGG | - | 43366:324292-324311 | MS.gene81946:intron | 55.0% |
| !! | GACTCGCTGACTCCTCAACA+TGG | - | 43366:323515-323534 | MS.gene81946:CDS | 55.0% |
| !! | GCAGGAAGCAGTATTGGCCA+AGG | - | 43366:324260-324279 | MS.gene81946:intron | 55.0% |
| CACACACGTGGCCCAAAACG+TGG | - | 43366:324084-324103 | MS.gene81946:intron | 60.0% | |
| CACACGTGGCCCAAAACGTG+GGG | - | 43366:324086-324105 | MS.gene81946:intron | 60.0% | |
| !! | GTTAGCTTGCAGCCGGCCAT+TGG | - | 43366:324299-324318 | MS.gene81946:intron | 60.0% |
| CCAAAACGTGGGGAGCCACG+CGG | - | 43366:324096-324115 | MS.gene81946:intron | 65.0% | |
| TGCGGGTGATAGGTACGCCC+TGG | - | 43366:324332-324351 | MS.gene81946:intron | 65.0% | |
| ! | CGCGTGGCTCCCCACGTTTT+GGG | + | 43366:324098-324117 | None:intergenic | 65.0% |
| ! | CCGCGTGGCTCCCCACGTTT+TGG | + | 43366:324099-324118 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 43366 | gene | 323437 | 324533 | 323437 | ID=MS.gene81946 |
| 43366 | mRNA | 323437 | 324533 | 323437 | ID=MS.gene81946.t1;Parent=MS.gene81946 |
| 43366 | exon | 324460 | 324533 | 324460 | ID=MS.gene81946.t1.exon1;Parent=MS.gene81946.t1 |
| 43366 | CDS | 324460 | 324533 | 324460 | ID=cds.MS.gene81946.t1;Parent=MS.gene81946.t1 |
| 43366 | exon | 323821 | 323947 | 323821 | ID=MS.gene81946.t1.exon2;Parent=MS.gene81946.t1 |
| 43366 | CDS | 323821 | 323947 | 323821 | ID=cds.MS.gene81946.t1;Parent=MS.gene81946.t1 |
| 43366 | exon | 323437 | 323742 | 323437 | ID=MS.gene81946.t1.exon3;Parent=MS.gene81946.t1 |
| 43366 | CDS | 323437 | 323742 | 323437 | ID=cds.MS.gene81946.t1;Parent=MS.gene81946.t1 |
| Gene Sequence |
| Protein sequence |
