AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81975


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81975.t1 MTR_3g090350 96.774 248 1 3 1 241 1 248 2.86e-169 466
MS.gene81975.t1 MTR_1g028910 44.583 240 81 7 6 239 6 199 6.75e-41 139
MS.gene81975.t1 MTR_8g093390 40.323 248 97 12 5 239 4 213 8.71e-38 131
MS.gene81975.t1 MTR_8g093335 40.323 248 97 12 5 239 4 213 8.71e-38 131
MS.gene81975.t1 MTR_1g028920 57.831 83 24 3 4 82 3 78 4.47e-21 85.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81975.t1 AT1G21326 37.008 254 116 8 11 240 4 237 2.80e-26 102
MS.gene81975.t1 AT3G18690 39.367 221 87 8 14 229 19 197 1.05e-23 95.5
MS.gene81975.t1 AT1G21320 36.098 205 84 6 11 184 4 192 1.81e-18 83.6
MS.gene81975.t1 AT1G21320 36.098 205 84 6 11 184 4 192 2.85e-18 83.6

Find 72 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTTATTCAACTATTTCTT+AGG 0.112026 3.2:+76501279 MS.gene81975:CDS
TCACTAGTAATACTTGTTAT+TGG 0.246006 3.2:-76500973 None:intergenic
GAGACGGTGTAGATTATAAT+TGG 0.248090 3.2:-76500742 None:intergenic
GAGAAAGCTATGTCTCCTTT+AGG 0.288459 3.2:+76500922 MS.gene81975:CDS
GGACCTTGAAGTTCTCTTCT+TGG 0.290039 3.2:-76500616 None:intergenic
GATCTTCCTGTAGGAATGTC+TGG 0.350042 3.2:-76500592 None:intergenic
AAATCAATAGAAGGAGTAAT+AGG 0.360331 3.2:-76501261 None:intergenic
AGGAAGTTGTTGAACATGTT+TGG 0.384511 3.2:-76501147 None:intergenic
AGAAAGCTATGTCTCCTTTA+GGG 0.426920 3.2:+76500923 MS.gene81975:CDS
ATTTATTCAACTATTTCTTA+GGG 0.435993 3.2:+76501280 MS.gene81975:CDS
ATAGGTGAAACAAAATTAGA+AGG 0.438530 3.2:-76501243 None:intergenic
GGTGTGGTGTGGATTACCTT+AGG 0.449367 3.2:-76500766 None:intergenic
CTTCTTGGTGATCTTCCTGT+AGG 0.456063 3.2:-76500601 None:intergenic
CGAGGAGGTGGACGAGGAGG+AGG 0.458285 3.2:-76500827 None:intergenic
TGTTGCTGCTGCGGCGCCAA+TGG 0.459417 3.2:-76500691 None:intergenic
CAAACACAATTGATGAATCA+TGG 0.462976 3.2:+76501024 MS.gene81975:CDS
TAGTTGAATAAATCAATAGA+AGG 0.468934 3.2:-76501270 None:intergenic
GGAGATAGTGAAGCCGGTCC+CGG 0.477212 3.2:-76501087 None:intergenic
AACCTCGTCCAACGTCTCAC+TGG 0.478033 3.2:+76500802 MS.gene81975:CDS
AGAGGAAACATGATGTTTCA+AGG 0.483946 3.2:+76501054 MS.gene81975:CDS
GTGTGGTGTGGATTACCTTA+GGG 0.486154 3.2:-76500765 None:intergenic
ATTGATCCTTTCCGCGGGGA+TGG 0.486778 3.2:+76500865 MS.gene81975:CDS
ATGGTGGCATAACGCGCCGC+GGG 0.509887 3.2:-76500898 None:intergenic
TCACCAAGAAGAGAACTTCA+AGG 0.519379 3.2:+76500613 MS.gene81975:CDS
TGCTGCTGCGGCGCCAATGG+TGG 0.524978 3.2:-76500688 None:intergenic
GAGTCTTTGTGTATTCTAAG+AGG 0.532916 3.2:-76500649 None:intergenic
TTGTGTATTCTAAGAGGTGT+AGG 0.534406 3.2:-76500643 None:intergenic
TTTCTACCACTATGAAGTGC+AGG 0.535371 3.2:-76501183 None:intergenic
CAATTCCCAGACATTCCTAC+AGG 0.536016 3.2:+76500586 MS.gene81975:CDS
TGAAGACGAGGAGGTGGACG+AGG 0.538450 3.2:-76500833 None:intergenic
AACATGTTTGGATCCGAAGA+TGG 0.542342 3.2:-76501135 None:intergenic
GACGAGGTTCATGAAGTCAC+CGG 0.543564 3.2:-76500788 None:intergenic
CAAATATTGATCCTTTCCGC+GGG 0.544079 3.2:+76500860 MS.gene81975:CDS
GTTCATGAAGTCACCGGGTG+TGG 0.544703 3.2:-76500782 None:intergenic
TGATGATGAAGACGAGGAGG+TGG 0.545268 3.2:-76500839 None:intergenic
TTAAGTCCTGCACTTCATAG+TGG 0.549789 3.2:+76501177 MS.gene81975:CDS
ATCTTCCTGTAGGAATGTCT+GGG 0.550841 3.2:-76500591 None:intergenic
ATGAATCATGGAGTAGAGAG+AGG 0.552023 3.2:+76501036 MS.gene81975:CDS
GAAACAAAATTAGAAGGACT+TGG 0.555617 3.2:-76501237 None:intergenic
CGGTGTAGATTATAATTGGT+TGG 0.555935 3.2:-76500738 None:intergenic
GAGGAAACATGATGTTTCAA+GGG 0.560217 3.2:+76501055 MS.gene81975:CDS
TGTTGTTGTTGTTGCTGCTG+CGG 0.561130 3.2:-76500700 None:intergenic
ATCCAGTGAGACGTTGGACG+AGG 0.565433 3.2:-76500804 None:intergenic
GGAATTGGAGATAGTGAAGC+CGG 0.565624 3.2:-76501093 None:intergenic
AATCTACACCGTCTCCCCTA+AGG 0.567138 3.2:+76500750 MS.gene81975:CDS
AAGGTAATCCACACCACACC+CGG 0.567522 3.2:+76500769 MS.gene81975:CDS
ATGTAATAAGTGACATGGAA+GGG 0.573767 3.2:+76500995 MS.gene81975:CDS
TCAAATATTGATCCTTTCCG+CGG 0.581682 3.2:+76500859 MS.gene81975:CDS
GTGCAGGACTTAAATCATGA+AGG 0.587314 3.2:-76501167 None:intergenic
TGAAGTCACCGGGTGTGGTG+TGG 0.588782 3.2:-76500777 None:intergenic
CAAAATCAAGAAACCACCAT+TGG 0.593308 3.2:+76500675 MS.gene81975:CDS
ATTGTTCCATCCCCGCGGAA+AGG 0.594293 3.2:-76500871 None:intergenic
GTGGATTACCTTAGGGGAGA+CGG 0.596921 3.2:-76500758 None:intergenic
AGGAGGATCCAGTGAGACGT+TGG 0.603439 3.2:-76500810 None:intergenic
CATGGTGGCATAACGCGCCG+CGG 0.605256 3.2:-76500899 None:intergenic
ATTCTAAGAGGTGTAGGACG+TGG 0.611632 3.2:-76500637 None:intergenic
GATGTAATAAGTGACATGGA+AGG 0.613311 3.2:+76500994 MS.gene81975:CDS
TGGTGGCATAACGCGCCGCG+GGG 0.623818 3.2:-76500897 None:intergenic
AGACGAGGAGGTGGACGAGG+AGG 0.631145 3.2:-76500830 None:intergenic
ACGAGGTTCATGAAGTCACC+GGG 0.632122 3.2:-76500787 None:intergenic
TAGATTATAATTGGTTGGCG+CGG 0.634687 3.2:-76500733 None:intergenic
GGTGGCATAACGCGCCGCGG+GGG 0.638082 3.2:-76500896 None:intergenic
TAGTGATGTAATAAGTGACA+TGG 0.644199 3.2:+76500990 MS.gene81975:CDS
ATGTTTGGATCCGAAGATGG+TGG 0.647910 3.2:-76501132 None:intergenic
CGCGGCGCGTTATGCCACCA+TGG 0.654579 3.2:+76500900 MS.gene81975:CDS
AGGAGACATAGCTTTCTCCA+TGG 0.670650 3.2:-76500917 None:intergenic
ATTTGATGATGAAGACGAGG+AGG 0.673773 3.2:-76500842 None:intergenic
TGTGGTGTGGATTACCTTAG+GGG 0.679590 3.2:-76500764 None:intergenic
AGACATAGCTTTCTCCATGG+TGG 0.695038 3.2:-76500914 None:intergenic
AATATTTGATGATGAAGACG+AGG 0.706902 3.2:-76500845 None:intergenic
AAATATTGATCCTTTCCGCG+GGG 0.729506 3.2:+76500861 MS.gene81975:CDS
GGGATATTGTTCCATCCCCG+CGG 0.741668 3.2:-76500876 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTATTCAACTATTTCTTA+GGG + chr3.2:76501280-76501299 MS.gene81975:CDS 15.0%
!! TAGTTGAATAAATCAATAGA+AGG - chr3.2:76501273-76501292 None:intergenic 20.0%
!!! GATTTATTCAACTATTTCTT+AGG + chr3.2:76501279-76501298 MS.gene81975:CDS 20.0%
! AAATCAATAGAAGGAGTAAT+AGG - chr3.2:76501264-76501283 None:intergenic 25.0%
! ATAGGTGAAACAAAATTAGA+AGG - chr3.2:76501246-76501265 None:intergenic 25.0%
! GAGAAAAAATTAGAAGGAAT+TGG - chr3.2:76501111-76501130 None:intergenic 25.0%
!! TCATAGTGGTAGAAATTTTA+TGG + chr3.2:76501191-76501210 MS.gene81975:CDS 25.0%
!!! TCACTAGTAATACTTGTTAT+TGG - chr3.2:76500976-76500995 None:intergenic 25.0%
ATGTAATAAGTGACATGGAA+GGG + chr3.2:76500995-76501014 MS.gene81975:CDS 30.0%
CAAACACAATTGATGAATCA+TGG + chr3.2:76501024-76501043 MS.gene81975:CDS 30.0%
GAAACAAAATTAGAAGGACT+TGG - chr3.2:76501240-76501259 None:intergenic 30.0%
TAGTGATGTAATAAGTGACA+TGG + chr3.2:76500990-76501009 MS.gene81975:CDS 30.0%
! AGTGGTAGAAATTTTATGGA+AGG + chr3.2:76501195-76501214 MS.gene81975:CDS 30.0%
!! AATATTTGATGATGAAGACG+AGG - chr3.2:76500848-76500867 None:intergenic 30.0%
!!! TGTTGTTGTTTTTTCCCTAA+AGG - chr3.2:76500940-76500959 None:intergenic 30.0%
AGAAAGCTATGTCTCCTTTA+GGG + chr3.2:76500923-76500942 MS.gene81975:CDS 35.0%
AGAGGAAACATGATGTTTCA+AGG + chr3.2:76501054-76501073 MS.gene81975:CDS 35.0%
AGGAAGTTGTTGAACATGTT+TGG - chr3.2:76501150-76501169 None:intergenic 35.0%
CAAAATCAAGAAACCACCAT+TGG + chr3.2:76500675-76500694 MS.gene81975:CDS 35.0%
CGGTGTAGATTATAATTGGT+TGG - chr3.2:76500741-76500760 None:intergenic 35.0%
GAGACGGTGTAGATTATAAT+TGG - chr3.2:76500745-76500764 None:intergenic 35.0%
GAGGAAACATGATGTTTCAA+GGG + chr3.2:76501055-76501074 MS.gene81975:CDS 35.0%
GAGTCTTTGTGTATTCTAAG+AGG - chr3.2:76500652-76500671 None:intergenic 35.0%
GATGTAATAAGTGACATGGA+AGG + chr3.2:76500994-76501013 MS.gene81975:CDS 35.0%
TAGATTATAATTGGTTGGCG+CGG - chr3.2:76500736-76500755 None:intergenic 35.0%
TCAAATATTGATCCTTTCCG+CGG + chr3.2:76500859-76500878 MS.gene81975:CDS 35.0%
! GGTAGAAATTTTATGGAAGG+TGG + chr3.2:76501198-76501217 MS.gene81975:CDS 35.0%
! GTTTCAAGGGATTTTATCTC+CGG + chr3.2:76501068-76501087 MS.gene81975:CDS 35.0%
! TTGTGTATTCTAAGAGGTGT+AGG - chr3.2:76500646-76500665 None:intergenic 35.0%
! TTTCAAGGGATTTTATCTCC+GGG + chr3.2:76501069-76501088 MS.gene81975:CDS 35.0%
AAATATTGATCCTTTCCGCG+GGG + chr3.2:76500861-76500880 MS.gene81975:CDS 40.0%
AACATGTTTGGATCCGAAGA+TGG - chr3.2:76501138-76501157 None:intergenic 40.0%
ATCTTCCTGTAGGAATGTCT+GGG - chr3.2:76500594-76500613 None:intergenic 40.0%
ATGAATCATGGAGTAGAGAG+AGG + chr3.2:76501036-76501055 MS.gene81975:CDS 40.0%
CAAATATTGATCCTTTCCGC+GGG + chr3.2:76500860-76500879 MS.gene81975:CDS 40.0%
GAGAAAGCTATGTCTCCTTT+AGG + chr3.2:76500922-76500941 MS.gene81975:CDS 40.0%
GGTGGCGAGAAAAAATTAGA+AGG - chr3.2:76501117-76501136 None:intergenic 40.0%
GTGCAGGACTTAAATCATGA+AGG - chr3.2:76501170-76501189 None:intergenic 40.0%
TCACCAAGAAGAGAACTTCA+AGG + chr3.2:76500613-76500632 MS.gene81975:CDS 40.0%
TTAAGTCCTGCACTTCATAG+TGG + chr3.2:76501177-76501196 MS.gene81975:CDS 40.0%
TTTCTACCACTATGAAGTGC+AGG - chr3.2:76501186-76501205 None:intergenic 40.0%
!! ATTTGATGATGAAGACGAGG+AGG - chr3.2:76500845-76500864 None:intergenic 40.0%
!!! TTTTTTCTCGCCACCATCTT+CGG + chr3.2:76501122-76501141 MS.gene81975:CDS 40.0%
AGACATAGCTTTCTCCATGG+TGG - chr3.2:76500917-76500936 None:intergenic 45.0%
AGGAGACATAGCTTTCTCCA+TGG - chr3.2:76500920-76500939 None:intergenic 45.0%
ATGTTTGGATCCGAAGATGG+TGG - chr3.2:76501135-76501154 None:intergenic 45.0%
CAATTCCCAGACATTCCTAC+AGG + chr3.2:76500586-76500605 MS.gene81975:CDS 45.0%
GATCTTCCTGTAGGAATGTC+TGG - chr3.2:76500595-76500614 None:intergenic 45.0%
GGAATTGGAGATAGTGAAGC+CGG - chr3.2:76501096-76501115 None:intergenic 45.0%
GGACCTTGAAGTTCTCTTCT+TGG - chr3.2:76500619-76500638 None:intergenic 45.0%
! ATTCTAAGAGGTGTAGGACG+TGG - chr3.2:76500640-76500659 None:intergenic 45.0%
! CTTCTTGGTGATCTTCCTGT+AGG - chr3.2:76500604-76500623 None:intergenic 45.0%
! GTGTGGTGTGGATTACCTTA+GGG - chr3.2:76500768-76500787 None:intergenic 45.0%
! TGTGGTGTGGATTACCTTAG+GGG - chr3.2:76500767-76500786 None:intergenic 45.0%
! TGTTGTTGTTGTTGCTGCTG+CGG - chr3.2:76500703-76500722 None:intergenic 45.0%
AAGGTAATCCACACCACACC+CGG + chr3.2:76500769-76500788 MS.gene81975:CDS 50.0%
AATCTACACCGTCTCCCCTA+AGG + chr3.2:76500750-76500769 MS.gene81975:CDS 50.0%
ACGAGGTTCATGAAGTCACC+GGG - chr3.2:76500790-76500809 None:intergenic 50.0%
GACGAGGTTCATGAAGTCAC+CGG - chr3.2:76500791-76500810 None:intergenic 50.0%
! AGGGATTTTATCTCCGGGAC+CGG + chr3.2:76501074-76501093 MS.gene81975:CDS 50.0%
! GGTGTGGTGTGGATTACCTT+AGG - chr3.2:76500769-76500788 None:intergenic 50.0%
! GTGGATTACCTTAGGGGAGA+CGG - chr3.2:76500761-76500780 None:intergenic 50.0%
! TGATGATGAAGACGAGGAGG+TGG - chr3.2:76500842-76500861 None:intergenic 50.0%
AACCTCGTCCAACGTCTCAC+TGG + chr3.2:76500802-76500821 MS.gene81975:CDS 55.0%
AGGAGGATCCAGTGAGACGT+TGG - chr3.2:76500813-76500832 None:intergenic 55.0%
ATCCAGTGAGACGTTGGACG+AGG - chr3.2:76500807-76500826 None:intergenic 55.0%
ATTGATCCTTTCCGCGGGGA+TGG + chr3.2:76500865-76500884 MS.gene81975:CDS 55.0%
ATTGTTCCATCCCCGCGGAA+AGG - chr3.2:76500874-76500893 None:intergenic 55.0%
GGATGGAACAATATCCCCCG+CGG + chr3.2:76500882-76500901 MS.gene81975:CDS 55.0%
GGGATATTGTTCCATCCCCG+CGG - chr3.2:76500879-76500898 None:intergenic 55.0%
GTTCATGAAGTCACCGGGTG+TGG - chr3.2:76500785-76500804 None:intergenic 55.0%
GGAGATAGTGAAGCCGGTCC+CGG - chr3.2:76501090-76501109 None:intergenic 60.0%
TGAAGACGAGGAGGTGGACG+AGG - chr3.2:76500836-76500855 None:intergenic 60.0%
TGAAGTCACCGGGTGTGGTG+TGG - chr3.2:76500780-76500799 None:intergenic 60.0%
AGACGAGGAGGTGGACGAGG+AGG - chr3.2:76500833-76500852 None:intergenic 65.0%
ATGGTGGCATAACGCGCCGC+GGG - chr3.2:76500901-76500920 None:intergenic 65.0%
CATGGTGGCATAACGCGCCG+CGG - chr3.2:76500902-76500921 None:intergenic 65.0%
TGTTGCTGCTGCGGCGCCAA+TGG - chr3.2:76500694-76500713 None:intergenic 65.0%
CGAGGAGGTGGACGAGGAGG+AGG - chr3.2:76500830-76500849 None:intergenic 70.0%
CGCGGCGCGTTATGCCACCA+TGG + chr3.2:76500900-76500919 MS.gene81975:CDS 70.0%
TGCTGCTGCGGCGCCAATGG+TGG - chr3.2:76500691-76500710 None:intergenic 70.0%
TGGTGGCATAACGCGCCGCG+GGG - chr3.2:76500900-76500919 None:intergenic 70.0%
GGTGGCATAACGCGCCGCGG+GGG - chr3.2:76500899-76500918 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 76500580 76501305 76500580 ID=MS.gene81975
chr3.2 mRNA 76500580 76501305 76500580 ID=MS.gene81975.t1;Parent=MS.gene81975
chr3.2 exon 76500580 76501305 76500580 ID=MS.gene81975.t1.exon1;Parent=MS.gene81975.t1
chr3.2 CDS 76500580 76501305 76500580 ID=cds.MS.gene81975.t1;Parent=MS.gene81975.t1
Gene Sequence

>MS.gene81975

ATGAATCAATTCCCAGACATTCCTACAGGAAGATCACCAAGAAGAGAACTTCAAGGTCCACGTCCTACACCTCTTAGAATACACAAAGACTCTCACAAAATCAAGAAACCACCATTGGCGCCGCAGCAGCAACAACAACAACAATATCAACAACCGCGCCAACCAATTATAATCTACACCGTCTCCCCTAAGGTAATCCACACCACACCCGGTGACTTCATGAACCTCGTCCAACGTCTCACTGGATCCTCCTCCTCGTCCACCTCCTCGTCTTCATCATCAAATATTGATCCTTTCCGCGGGGATGGAACAATATCCCCCGCGGCGCGTTATGCCACCATGGAGAAAGCTATGTCTCCTTTAGGGAAAAAACAACAACAACAAATAGTAGCACCAATAACAAGTATTACTAGTGATGTAATAAGTGACATGGAAGGGATAGAACAAACACAATTGATGAATCATGGAGTAGAGAGAGGAAACATGATGTTTCAAGGGATTTTATCTCCGGGACCGGCTTCACTATCTCCAATTCCTTCTAATTTTTTCTCGCCACCATCTTCGGATCCAAACATGTTCAACAACTTCCTTCATGATTTAAGTCCTGCACTTCATAGTGGTAGAAATTTTATGGAAGGTGGTGCTAATTTCTTATTACCAAGTCCTTCTAATTTTGTTTCACCTATTACTCCTTCTATTGATTTATTCAACTATTTCTTAGGGTAA

Protein sequence

>MS.gene81975.t1

MNQFPDIPTGRSPRRELQGPRPTPLRIHKDSHKIKKPPLAPQQQQQQQYQQPRQPIIIYTVSPKVIHTTPGDFMNLVQRLTGSSSSSTSSSSSSNIDPFRGDGTISPAARYATMEKAMSPLGKKQQQQIVAPITSITSDVISDMEGIEQTQLMNHGVERGNMMFQGILSPGPASLSPIPSNFFSPPSSDPNMFNNFLHDLSPALHSGRNFMEGGANFLLPSPSNFVSPITPSIDLFNYFLG