Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82183.t1 | KEH29169.1 | 95.3 | 232 | 11 | 0 | 1 | 232 | 1 | 232 | 5.30E-118 | 433.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82183.t1 | A0A072UHD2 | 95.3 | 232 | 11 | 0 | 1 | 232 | 1 | 232 | 3.8e-118 | 433.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene82183 | 0.860563 | 1.89E-63 | -1.69E-46 |
| MS.gene050096 | MS.gene82183 | 0.803253 | 3.81E-49 | -1.69E-46 |
| MS.gene051131 | MS.gene82183 | 0.837821 | 4.15E-57 | -1.69E-46 |
| MS.gene052211 | MS.gene82183 | 0.810355 | 1.20E-50 | -1.69E-46 |
| MS.gene05240 | MS.gene82183 | 0.809509 | 1.83E-50 | -1.69E-46 |
| MS.gene053403 | MS.gene82183 | 0.801405 | 9.15E-49 | -1.69E-46 |
| MS.gene054146 | MS.gene82183 | 0.832434 | 9.48E-56 | -1.69E-46 |
| MS.gene055240 | MS.gene82183 | 0.806957 | 6.39E-50 | -1.69E-46 |
| MS.gene058603 | MS.gene82183 | 0.806504 | 7.97E-50 | -1.69E-46 |
| MS.gene059265 | MS.gene82183 | 0.940509 | 2.07E-100 | -1.69E-46 |
| MS.gene059955 | MS.gene82183 | 0.835205 | 1.92E-56 | -1.69E-46 |
| MS.gene061092 | MS.gene82183 | 0.811613 | 6.41E-51 | -1.69E-46 |
| MS.gene061094 | MS.gene82183 | 0.820107 | 8.17E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82183.t1 | MTR_4g027195 | 95.259 | 232 | 11 | 0 | 1 | 232 | 1 | 232 | 8.20e-157 | 434 |
| MS.gene82183.t1 | MTR_4g027105 | 68.421 | 171 | 48 | 3 | 60 | 228 | 25 | 191 | 2.98e-71 | 216 |
| MS.gene82183.t1 | MTR_4g027225 | 66.667 | 171 | 51 | 3 | 60 | 228 | 25 | 191 | 1.93e-59 | 186 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTTGCCCTTCTTCTCTT+TGG | 0.204053 | 4.2:-76288552 | MS.gene82183:CDS |
| TCAACTAGGTCAGCTGGATT+TGG | 0.237066 | 4.2:+76288312 | None:intergenic |
| TGACCATATTGTATTGGATT+TGG | 0.293386 | 4.2:-76289450 | MS.gene82183:CDS |
| TCGTCACTCGCTTAAAGTTT+TGG | 0.323243 | 4.2:-76288433 | MS.gene82183:CDS |
| TAGATTACCAACTACTTTGA+AGG | 0.357772 | 4.2:-76288799 | MS.gene82183:CDS |
| TCTCTTTGGAATACTTTCTT+CGG | 0.374925 | 4.2:-76288538 | MS.gene82183:CDS |
| CTTCCAAATCCAATACAATA+TGG | 0.375006 | 4.2:+76289447 | None:intergenic |
| CAACTAGGTCAGCTGGATTT+GGG | 0.375194 | 4.2:+76288313 | None:intergenic |
| TGCATCTACTGCTCGCTAAT+CGG | 0.377217 | 4.2:-76288624 | MS.gene82183:CDS |
| TTCTAATTCTGCATACTGAT+AGG | 0.378805 | 4.2:+76289399 | None:intergenic |
| TTTGGGTTGTGCTCTTCGTA+TGG | 0.396092 | 4.2:-76288479 | MS.gene82183:CDS |
| TTGGGTTGTGCTCTTCGTAT+GGG | 0.402906 | 4.2:-76288478 | MS.gene82183:CDS |
| AAAGTATTCCAAAGAGAAGA+AGG | 0.428632 | 4.2:+76288544 | None:intergenic |
| GGTGTTGATATTCAATACTA+AGG | 0.429220 | 4.2:-76288367 | MS.gene82183:CDS |
| TCTTGATCCTTCAAAGTAGT+TGG | 0.429294 | 4.2:+76288792 | None:intergenic |
| GAGTGACGAGCAAGTATAAC+TGG | 0.443034 | 4.2:+76288447 | None:intergenic |
| ATGGCATGGCATGGGATTGA+AGG | 0.461512 | 4.2:+76288762 | None:intergenic |
| CTACCTTCAACTAGGTCAGC+TGG | 0.475286 | 4.2:+76288306 | None:intergenic |
| AATCCAGCTGACCTAGTTGA+AGG | 0.483463 | 4.2:-76288309 | MS.gene82183:CDS |
| TTTCTTGACACAATACTCAT+TGG | 0.492166 | 4.2:-76288732 | MS.gene82183:CDS |
| GGAAGAACATGAAGATGTAA+AGG | 0.495227 | 4.2:-76289429 | MS.gene82183:CDS |
| ATGAACTTTGTGATCATCAA+AGG | 0.497786 | 4.2:+76288671 | None:intergenic |
| AACACAAAGATGGCATGGCA+TGG | 0.520422 | 4.2:+76288753 | None:intergenic |
| GTGTAATGACCATATTGTAT+TGG | 0.548354 | 4.2:-76289456 | MS.gene82183:CDS |
| ACACAAAGATGGCATGGCAT+GGG | 0.550895 | 4.2:+76288754 | None:intergenic |
| CGGCAGCAAGATGGAGAGAA+GGG | 0.553496 | 4.2:+76288512 | None:intergenic |
| TGGGTTGTGCTCTTCGTATG+GGG | 0.556531 | 4.2:-76288477 | MS.gene82183:CDS |
| AAGTTGTCGCGGCAGCAAGA+TGG | 0.558737 | 4.2:+76288503 | None:intergenic |
| TGAACTTTGTGATCATCAAA+GGG | 0.562970 | 4.2:+76288672 | None:intergenic |
| GATGCAGATGGATGTCAAGA+AGG | 0.564412 | 4.2:+76288641 | None:intergenic |
| ATTAAGATCTACCTTCAACT+AGG | 0.573127 | 4.2:+76288298 | None:intergenic |
| TGGCATGGCATGGGATTGAA+GGG | 0.575309 | 4.2:+76288763 | None:intergenic |
| TAGGTCAGCTGGATTTGGGT+AGG | 0.578541 | 4.2:+76288317 | None:intergenic |
| TGTGTCAAGAAACACAAAGA+TGG | 0.584873 | 4.2:+76288743 | None:intergenic |
| CTTGATTATAGTATTTCACA+CGG | 0.587536 | 4.2:+76289312 | None:intergenic |
| CAAGAAACACAAAGATGGCA+TGG | 0.592259 | 4.2:+76288748 | None:intergenic |
| GCGGCAGCAAGATGGAGAGA+AGG | 0.593311 | 4.2:+76288511 | None:intergenic |
| AAGTATTCCAAAGAGAAGAA+GGG | 0.599617 | 4.2:+76288545 | None:intergenic |
| GTGTCGGATAATAGAAGCGA+TGG | 0.602232 | 4.2:-76288409 | MS.gene82183:CDS |
| TAGCGAGCAGTAGATGCAGA+TGG | 0.605364 | 4.2:+76288629 | None:intergenic |
| TGAAATACTATAATCAAGCA+AGG | 0.609382 | 4.2:-76289307 | MS.gene82183:intron |
| GGTAGTAAAGATTACTACCA+TGG | 0.620964 | 4.2:-76288388 | MS.gene82183:CDS |
| AATACTAAGGATTCAGCCAG+TGG | 0.626440 | 4.2:-76288354 | MS.gene82183:CDS |
| AAGATCTACACGAAGACTCG+TGG | 0.646014 | 4.2:-76288597 | MS.gene82183:CDS |
| TTGATTATAGTATTTCACAC+GGG | 0.658993 | 4.2:+76289313 | None:intergenic |
| AGTATTGAATATCAACACCA+TGG | 0.687774 | 4.2:+76288371 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATATTAATTTAATAGTAC+TGG | - | chr4.2:76288739-76288758 | MS.gene82183:CDS | 10.0% |
| !! | CAATTAAAATAAATAAAAGA+GGG | + | chr4.2:76288808-76288827 | None:intergenic | 10.0% |
| !!! | ATATGTTTTATATATCTAAT+CGG | - | chr4.2:76288935-76288954 | MS.gene82183:intron | 10.0% |
| !!! | CTCTTTTATTTATTTTAATT+GGG | - | chr4.2:76288807-76288826 | MS.gene82183:intron | 10.0% |
| !! | ACATTGTCAAAAAAAAAATT+AGG | + | chr4.2:76288902-76288921 | None:intergenic | 15.0% |
| !! | CATTGTCAAAAAAAAAATTA+GGG | + | chr4.2:76288901-76288920 | None:intergenic | 15.0% |
| !! | CCAATTAAAATAAATAAAAG+AGG | + | chr4.2:76288809-76288828 | None:intergenic | 15.0% |
| !!! | CCTCTTTTATTTATTTTAAT+TGG | - | chr4.2:76288806-76288825 | MS.gene82183:intron | 15.0% |
| !!! | TAGCCTTATTTAAATATTTT+GGG | + | chr4.2:76288697-76288716 | None:intergenic | 15.0% |
| !!! | TATTATTTTGTTTTTTTGAC+TGG | - | chr4.2:76288523-76288542 | MS.gene82183:CDS | 15.0% |
| !!! | TATTTTTGTTTATTATGGTT+TGG | - | chr4.2:76288857-76288876 | MS.gene82183:intron | 15.0% |
| !!! | TTTTTATGATACTTTGTTTA+CGG | + | chr4.2:76288647-76288666 | None:intergenic | 15.0% |
| !! | AGTGAAGAATCATATAAAAT+AGG | + | chr4.2:76288671-76288690 | None:intergenic | 20.0% |
| !! | GAACCCAAAATATTTAAATA+AGG | - | chr4.2:76288691-76288710 | MS.gene82183:CDS | 20.0% |
| !! | GTGAAGAATCATATAAAATA+GGG | + | chr4.2:76288670-76288689 | None:intergenic | 20.0% |
| !! | TAATTACTCTTTCCATTATA+TGG | - | chr4.2:76288482-76288501 | MS.gene82183:CDS | 20.0% |
| !!! | AGAACTTTTAAACCATATAA+TGG | + | chr4.2:76288497-76288516 | None:intergenic | 20.0% |
| !!! | CTAGCCTTATTTAAATATTT+TGG | + | chr4.2:76288698-76288717 | None:intergenic | 20.0% |
| !!! | GCACATATTTTTGTTTATTA+TGG | - | chr4.2:76288852-76288871 | MS.gene82183:intron | 20.0% |
| !!! | TTTTGACAATGTTTTATGTA+TGG | - | chr4.2:76288910-76288929 | MS.gene82183:intron | 20.0% |
| ! | AAAAGAGGGTAGTTAATAAA+AGG | + | chr4.2:76288794-76288813 | None:intergenic | 25.0% |
| ! | CTTGATTATAGTATTTCACA+CGG | + | chr4.2:76288454-76288473 | None:intergenic | 25.0% |
| ! | TACGGATTTGATTAAACATA+AGG | + | chr4.2:76288629-76288648 | None:intergenic | 25.0% |
| ! | TAGAAAAAGAAGACTTAAAG+TGG | - | chr4.2:76288382-76288401 | MS.gene82183:CDS | 25.0% |
| ! | TGAAATACTATAATCAAGCA+AGG | - | chr4.2:76288456-76288475 | MS.gene82183:CDS | 25.0% |
| ! | TTGATTATAGTATTTCACAC+GGG | + | chr4.2:76288453-76288472 | None:intergenic | 25.0% |
| !! | TCACTAACTTCAATTTTCTT+TGG | + | chr4.2:76288421-76288440 | None:intergenic | 25.0% |
| !!! | CAAAGCTAAGAATTTTTTCT+TGG | + | chr4.2:76289198-76289217 | None:intergenic | 25.0% |
| AAGTATTCCAAAGAGAAGAA+GGG | + | chr4.2:76289221-76289240 | None:intergenic | 30.0% | |
| ATGAACTTTGTGATCATCAA+AGG | + | chr4.2:76289095-76289114 | None:intergenic | 30.0% | |
| CTTCCAAATCCAATACAATA+TGG | + | chr4.2:76288319-76288338 | None:intergenic | 30.0% | |
| GTGTAATGACCATATTGTAT+TGG | - | chr4.2:76288307-76288326 | MS.gene82183:CDS | 30.0% | |
| TAGATTACCAACTACTTTGA+AGG | - | chr4.2:76288964-76288983 | MS.gene82183:intron | 30.0% | |
| TGAACTTTGTGATCATCAAA+GGG | + | chr4.2:76289094-76289113 | None:intergenic | 30.0% | |
| TGACCATATTGTATTGGATT+TGG | - | chr4.2:76288313-76288332 | MS.gene82183:CDS | 30.0% | |
| TTCTAATTCTGCATACTGAT+AGG | + | chr4.2:76288367-76288386 | None:intergenic | 30.0% | |
| TTTCTTGACACAATACTCAT+TGG | - | chr4.2:76289031-76289050 | MS.gene82183:intron | 30.0% | |
| ! | AAAGTATTCCAAAGAGAAGA+AGG | + | chr4.2:76289222-76289241 | None:intergenic | 30.0% |
| ! | AGTATTGAATATCAACACCA+TGG | + | chr4.2:76289395-76289414 | None:intergenic | 30.0% |
| !! | GGTGTTGATATTCAATACTA+AGG | - | chr4.2:76289396-76289415 | MS.gene82183:CDS | 30.0% |
| !! | TCTCTTTGGAATACTTTCTT+CGG | - | chr4.2:76289225-76289244 | MS.gene82183:intron | 30.0% |
| !! | TTTTCTTTGGTTTACTCTCA+TGG | + | chr4.2:76288408-76288427 | None:intergenic | 30.0% |
| !!! | AGCTAAGAATTTTTTCTTGG+TGG | + | chr4.2:76289195-76289214 | None:intergenic | 30.0% |
| GGAAGAACATGAAGATGTAA+AGG | - | chr4.2:76288334-76288353 | MS.gene82183:CDS | 35.0% | |
| GGGTCAAATTTCTAACAACT+AGG | - | chr4.2:76288827-76288846 | MS.gene82183:intron | 35.0% | |
| GGTAGTAAAGATTACTACCA+TGG | - | chr4.2:76289375-76289394 | MS.gene82183:CDS | 35.0% | |
| TCTTGATCCTTCAAAGTAGT+TGG | + | chr4.2:76288974-76288993 | None:intergenic | 35.0% | |
| TGTGTCAAGAAACACAAAGA+TGG | + | chr4.2:76289023-76289042 | None:intergenic | 35.0% | |
| AATACTAAGGATTCAGCCAG+TGG | - | chr4.2:76289409-76289428 | MS.gene82183:CDS | 40.0% | |
| ACAACCCAAAAAAGTTGTCG+CGG | + | chr4.2:76289274-76289293 | None:intergenic | 40.0% | |
| CAAGAAACACAAAGATGGCA+TGG | + | chr4.2:76289018-76289037 | None:intergenic | 40.0% | |
| ! | TCGTCACTCGCTTAAAGTTT+TGG | - | chr4.2:76289330-76289349 | MS.gene82183:CDS | 40.0% |
| !! | CGCTTAAAGTTTTGGTGTGT+CGG | - | chr4.2:76289338-76289357 | MS.gene82183:CDS | 40.0% |
| AACACAAAGATGGCATGGCA+TGG | + | chr4.2:76289013-76289032 | None:intergenic | 45.0% | |
| AAGATCTACACGAAGACTCG+TGG | - | chr4.2:76289166-76289185 | MS.gene82183:intron | 45.0% | |
| AATCCAGCTGACCTAGTTGA+AGG | - | chr4.2:76289454-76289473 | MS.gene82183:CDS | 45.0% | |
| ACACAAAGATGGCATGGCAT+GGG | + | chr4.2:76289012-76289031 | None:intergenic | 45.0% | |
| AGCTTTGCCCTTCTTCTCTT+TGG | - | chr4.2:76289211-76289230 | MS.gene82183:intron | 45.0% | |
| CAACTAGGTCAGCTGGATTT+GGG | + | chr4.2:76289453-76289472 | None:intergenic | 45.0% | |
| GAGTGACGAGCAAGTATAAC+TGG | + | chr4.2:76289319-76289338 | None:intergenic | 45.0% | |
| GATGCAGATGGATGTCAAGA+AGG | + | chr4.2:76289125-76289144 | None:intergenic | 45.0% | |
| GTGTCGGATAATAGAAGCGA+TGG | - | chr4.2:76289354-76289373 | MS.gene82183:CDS | 45.0% | |
| TCAACTAGGTCAGCTGGATT+TGG | + | chr4.2:76289454-76289473 | None:intergenic | 45.0% | |
| TGCATCTACTGCTCGCTAAT+CGG | - | chr4.2:76289139-76289158 | MS.gene82183:intron | 45.0% | |
| ! | TTGGGTTGTGCTCTTCGTAT+GGG | - | chr4.2:76289285-76289304 | MS.gene82183:intron | 45.0% |
| !! | GGTTGTTTTGTTGCTTCCAC+TGG | + | chr4.2:76289428-76289447 | None:intergenic | 45.0% |
| !! | TTTGGGTTGTGCTCTTCGTA+TGG | - | chr4.2:76289284-76289303 | MS.gene82183:intron | 45.0% |
| ATGGCATGGCATGGGATTGA+AGG | + | chr4.2:76289004-76289023 | None:intergenic | 50.0% | |
| CTACCTTCAACTAGGTCAGC+TGG | + | chr4.2:76289460-76289479 | None:intergenic | 50.0% | |
| TAGCGAGCAGTAGATGCAGA+TGG | + | chr4.2:76289137-76289156 | None:intergenic | 50.0% | |
| TAGGTCAGCTGGATTTGGGT+AGG | + | chr4.2:76289449-76289468 | None:intergenic | 50.0% | |
| TGGCATGGCATGGGATTGAA+GGG | + | chr4.2:76289003-76289022 | None:intergenic | 50.0% | |
| TGGGTTGTGCTCTTCGTATG+GGG | - | chr4.2:76289286-76289305 | MS.gene82183:intron | 50.0% | |
| !!! | GCTGCCGCGACAACTTTTTT+GGG | - | chr4.2:76289267-76289286 | MS.gene82183:intron | 50.0% |
| !!! | TGCTGCCGCGACAACTTTTT+TGG | - | chr4.2:76289266-76289285 | MS.gene82183:intron | 50.0% |
| AAGTTGTCGCGGCAGCAAGA+TGG | + | chr4.2:76289263-76289282 | None:intergenic | 55.0% | |
| CGGCAGCAAGATGGAGAGAA+GGG | + | chr4.2:76289254-76289273 | None:intergenic | 55.0% | |
| GCGGCAGCAAGATGGAGAGA+AGG | + | chr4.2:76289255-76289274 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 76288299 | 76289486 | 76288299 | ID=MS.gene82183 |
| chr4.2 | mRNA | 76288299 | 76289486 | 76288299 | ID=MS.gene82183.t1;Parent=MS.gene82183 |
| chr4.2 | exon | 76289308 | 76289486 | 76289308 | ID=MS.gene82183.t1.exon1;Parent=MS.gene82183.t1 |
| chr4.2 | CDS | 76289308 | 76289486 | 76289308 | ID=cds.MS.gene82183.t1;Parent=MS.gene82183.t1 |
| chr4.2 | exon | 76288299 | 76288818 | 76288299 | ID=MS.gene82183.t1.exon2;Parent=MS.gene82183.t1 |
| chr4.2 | CDS | 76288299 | 76288818 | 76288299 | ID=cds.MS.gene82183.t1;Parent=MS.gene82183.t1 |
| Gene Sequence |
| Protein sequence |
