AlfalfaGEDB | MS.gene82218

M. sativa cultivar XinJiangDaYe / MS.gene82218

KEGG: -
GO: GO:0003674 GO:0005488 GO:0006810 GO:0006869 GO:0008150 GO:0008289 GO:0051179 GO:0051234 GO:0071702
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82218.t1	XP_003605308.1	84.7	111	16	1	4	113	3	113	1.30E-43	185.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82218.t1	A0AT31	39.3	107	63	2	4	109	7	112	2.2e-12	73.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82218.t1	G7JIC9	84.7	111	16	1	4	113	3	113	9.1e-44	185.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049758	MS.gene82218	0.806274	8.91E-50	-1.69E-46
MS.gene050301	MS.gene82218	0.834004	3.85E-56	-1.69E-46
MS.gene052178	MS.gene82218	0.815022	1.14E-51	-1.69E-46
MS.gene052188	MS.gene82218	0.812292	4.56E-51	-1.69E-46
MS.gene052310	MS.gene82218	0.801846	7.43E-49	-1.69E-46
MS.gene052340	MS.gene82218	0.82376	1.16E-53	-1.69E-46
MS.gene053209	MS.gene82218	0.80528	1.44E-49	-1.69E-46
MS.gene053557	MS.gene82218	0.815729	7.96E-52	-1.69E-46
MS.gene053836	MS.gene82218	0.802604	5.18E-49	-1.69E-46
MS.gene055116	MS.gene82218	0.840159	1.03E-57	-1.69E-46
MS.gene055604	MS.gene82218	0.804441	2.16E-49	-1.69E-46
MS.gene056191	MS.gene82218	0.803756	2.99E-49	-1.69E-46
MS.gene05811	MS.gene82218	0.81471	1.34E-51	-1.69E-46
MS.gene059688	MS.gene82218	0.824758	6.78E-54	-1.69E-46
MS.gene059692	MS.gene82218	0.837299	5.64E-57	-1.69E-46
MS.gene059695	MS.gene82218	0.841668	4.13E-58	-1.69E-46
MS.gene061049	MS.gene82218	0.827676	1.37E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82218.t1	MTR_4g028360	84.685	111	16	1	4	113	3	113	1.28e-55	169
MS.gene82218.t1	MTR_4g027800	35.514	107	67	2	4	109	6	111	1.19e-20	80.5
MS.gene82218.t1	MTR_4g027840	36.449	107	66	2	4	109	6	111	2.16e-20	80.5
MS.gene82218.t1	MTR_4g428370	37.383	107	65	2	4	109	63	168	2.05e-19	79.0
MS.gene82218.t1	MTR_4g028310	40.517	116	56	3	4	111	6	116	3.53e-17	72.0
MS.gene82218.t1	MTR_4g029350	35.455	110	68	2	7	114	9	117	6.60e-17	70.9
MS.gene82218.t1	MTR_4g029390	34.513	113	71	2	4	114	6	117	1.44e-16	70.1
MS.gene82218.t1	MTR_4g028190	29.204	113	78	1	4	114	10	122	3.77e-15	66.6
MS.gene82218.t1	MTR_7g073170	32.143	112	74	2	4	114	9	119	1.62e-14	64.7
MS.gene82218.t1	MTR_7g072830	35.455	110	64	3	4	111	14	118	4.64e-13	61.2
MS.gene82218.t1	MTR_7g073120	31.304	115	77	2	4	116	10	124	1.16e-12	60.8
MS.gene82218.t1	MTR_7g073100	32.759	116	73	2	4	116	9	122	2.18e-12	60.1
MS.gene82218.t1	MTR_7g072930	30.357	112	74	1	4	115	14	121	4.53e-12	59.3
MS.gene82218.t1	MTR_7g072993	30.172	116	78	2	4	116	9	124	6.44e-12	58.5
MS.gene82218.t1	MTR_7g072980	28.696	115	80	2	4	116	9	123	8.29e-12	58.2
MS.gene82218.t1	MTR_7g072900	28.440	109	77	1	4	111	9	117	2.88e-11	56.2
MS.gene82218.t1	MTR_1g040335	32.727	110	71	3	4	111	9	117	4.71e-11	55.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82218.t1	AT5G59320	32.143	112	72	3	1	111	5	113	5.00e-13	60.8
MS.gene82218.t1	AT2G15050	30.172	116	75	4	4	113	7	122	1.20e-12	60.1
MS.gene82218.t1	AT2G38540	32.727	110	68	4	4	109	7	114	1.08e-11	57.8

Find 19 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCACTGCTTCACAACAATA+TGG	0.222130	4.2:-75532512	None:intergenic
GAATACCACATAAGGCAAAT+AGG	0.312154	4.2:-75532643	None:intergenic
AATCTATCAGCTTCTGCAAT+AGG	0.389891	4.2:-75532422	None:intergenic
AGTAAGAGTATAAATAATAA+AGG	0.397290	4.2:-75532447	None:intergenic
AATACCACATAAGGCAAATA+GGG	0.405661	4.2:-75532642	None:intergenic
GGTCGTTGCATGTCTCGTTT+TGG	0.415995	4.2:+75532379	MS.gene82218:CDS
GAAATATAACCTTGAAGAAA+AGG	0.420263	4.2:-75532479	None:intergenic
AGTTGATATCGCGAGTAATC+TGG	0.431895	4.2:-75532676	None:intergenic
TTGTGACTGCTTGAGAGATA+TGG	0.453187	4.2:+75532580	MS.gene82218:CDS
TCACTGCTTCACAACAATAT+GGG	0.474158	4.2:-75532511	None:intergenic
GCTGCCCTATTTGCCTTATG+TGG	0.498988	4.2:+75532638	MS.gene82218:CDS
GGGTGTTTGAATACCACATA+AGG	0.517200	4.2:-75532651	None:intergenic
CCTTGAAGAAAAGGATAGCA+TGG	0.525784	4.2:-75532470	None:intergenic
TTCTTTAAAGACTTTGAGTG+TGG	0.544723	4.2:+75532607	MS.gene82218:CDS
TTAGTGAAAGTATACATACT+TGG	0.567662	4.2:-75532702	None:intergenic
AAGACTTTGAGTGTGGAGAA+TGG	0.572741	4.2:+75532614	MS.gene82218:CDS
GATATCGCGAGTAATCTGGT+AGG	0.618889	4.2:-75532672	None:intergenic
ATATCGCGAGTAATCTGGTA+GGG	0.629903	4.2:-75532671	None:intergenic
TAGACGATCATCATAGTCGA+TGG	0.640137	4.2:-75532552	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AGTAAGAGTATAAATAATAA+AGG	-	chr4.2:75532450-75532469	None:intergenic	15.0%
!	GAAATATAACCTTGAAGAAA+AGG	-	chr4.2:75532482-75532501	None:intergenic	25.0%
!!	TTAGTGAAAGTATACATACT+TGG	-	chr4.2:75532705-75532724	None:intergenic	25.0%
	AATACCACATAAGGCAAATA+GGG	-	chr4.2:75532645-75532664	None:intergenic	30.0%
!	TTCTTTAAAGACTTTGAGTG+TGG	+	chr4.2:75532607-75532626	MS.gene82218:CDS	30.0%
	AATCTATCAGCTTCTGCAAT+AGG	-	chr4.2:75532425-75532444	None:intergenic	35.0%
	GAATACCACATAAGGCAAAT+AGG	-	chr4.2:75532646-75532665	None:intergenic	35.0%
	TCACTGCTTCACAACAATAT+GGG	-	chr4.2:75532514-75532533	None:intergenic	35.0%
	TTCACTGCTTCACAACAATA+TGG	-	chr4.2:75532515-75532534	None:intergenic	35.0%
	AAGACTTTGAGTGTGGAGAA+TGG	+	chr4.2:75532614-75532633	MS.gene82218:CDS	40.0%
	ATATCGCGAGTAATCTGGTA+GGG	-	chr4.2:75532674-75532693	None:intergenic	40.0%
	CCTTGAAGAAAAGGATAGCA+TGG	-	chr4.2:75532473-75532492	None:intergenic	40.0%
	GGGTGTTTGAATACCACATA+AGG	-	chr4.2:75532654-75532673	None:intergenic	40.0%
	TAGACGATCATCATAGTCGA+TGG	-	chr4.2:75532555-75532574	None:intergenic	40.0%
	TTGTGACTGCTTGAGAGATA+TGG	+	chr4.2:75532580-75532599	MS.gene82218:CDS	40.0%
!	AGTTGATATCGCGAGTAATC+TGG	-	chr4.2:75532679-75532698	None:intergenic	40.0%
!	CCATGCTATCCTTTTCTTCA+AGG	+	chr4.2:75532470-75532489	MS.gene82218:CDS	40.0%
	GATATCGCGAGTAATCTGGT+AGG	-	chr4.2:75532675-75532694	None:intergenic	45.0%
	GCTGCCCTATTTGCCTTATG+TGG	+	chr4.2:75532638-75532657	MS.gene82218:CDS	50.0%
!	GGTCGTTGCATGTCTCGTTT+TGG	+	chr4.2:75532379-75532398	MS.gene82218:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	75532374	75532724	75532374	ID=MS.gene82218
chr4.2	mRNA	75532374	75532724	75532374	ID=MS.gene82218.t1;Parent=MS.gene82218
chr4.2	exon	75532374	75532724	75532374	ID=MS.gene82218.t1.exon1;Parent=MS.gene82218.t1
chr4.2	CDS	75532374	75532724	75532374	ID=cds.MS.gene82218.t1;Parent=MS.gene82218.t1

Gene Sequence	>MS.gene82218 ATGAAGGTCGTTGCATGTCTCGTTTTGGTGTTGTGCATTGTTGTTGCACCTATTGCAGAAGCTGATAGATTTGCCTTTATTATTTATACTCTTACTCCATGCTATCCTTTTCTTCAAGGTTATATTTCAACTGCTGACCCATATTGTTGTGAAGCAGTGAAGAAAGTTAACGATGACGCCATCGACTATGATGATCGTCTAGAAACTTGTGACTGCTTGAGAGATATGGCTCTTTCTTTAAAGACTTTGAGTGTGGAGAATGGTGCTGCCCTATTTGCCTTATGTGGTATTCAAACACCCTACCAGATTACTCGCGATATCAACTGTACCAAGTATGTATACTTTCACTAA
Protein sequence	>MS.gene82218.t1 MKVVACLVLVLCIVVAPIAEADRFAFIIYTLTPCYPFLQGYISTADPYCCEAVKKVNDDAIDYDDRLETCDCLRDMALSLKTLSVENGAALFALCGIQTPYQITRDINCTKYVYFH