AlfalfaGEDB | MS.gene82451

M. sativa cultivar XinJiangDaYe / MS.gene82451

KEGG: K02183
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF13499.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82451.t1	XP_003612498.1	97	200	6	0	1	200	1	200	2.90E-88	334.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82451.t1	Q93Z27	39.7	194	108	4	14	200	13	204	4.3e-24	112.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82451.t1	G7K424	97.0	200	6	0	1	200	1	200	2.1e-88	334.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049283	MS.gene82451	0.83471	2.56E-56	-1.69E-46
MS.gene049983	MS.gene82451	0.821324	4.29E-53	-1.69E-46
MS.gene04998	MS.gene82451	0.817217	3.70E-52	-1.69E-46
MS.gene050271	MS.gene82451	0.812284	4.58E-51	-1.69E-46
MS.gene051275	MS.gene82451	0.80775	4.34E-50	-1.69E-46
MS.gene051597	MS.gene82451	0.805982	1.03E-49	-1.69E-46
MS.gene053298	MS.gene82451	0.82899	6.61E-55	-1.69E-46
MS.gene053963	MS.gene82451	0.852317	5.00E-61	-1.69E-46
MS.gene05439	MS.gene82451	0.818299	2.11E-52	-1.69E-46
MS.gene054892	MS.gene82451	0.827538	1.48E-54	-1.69E-46
MS.gene054893	MS.gene82451	0.831713	1.43E-55	-1.69E-46
MS.gene055222	MS.gene82451	0.815978	7.01E-52	-1.69E-46
MS.gene056849	MS.gene82451	0.814854	1.25E-51	-1.69E-46
MS.gene057155	MS.gene82451	0.827048	1.94E-54	-1.69E-46
MS.gene057622	MS.gene82451	0.815686	8.14E-52	-1.69E-46
MS.gene057999	MS.gene82451	0.818258	2.15E-52	-1.69E-46
MS.gene058223	MS.gene82451	-0.802836	4.64E-49	-1.69E-46
MS.gene058592	MS.gene82451	0.81236	4.41E-51	-1.69E-46
MS.gene05952	MS.gene82451	0.823841	1.11E-53	-1.69E-46
MS.gene060259	MS.gene82451	0.835776	1.38E-56	-1.69E-46
MS.gene060412	MS.gene82451	0.808936	2.42E-50	-1.69E-46
MS.gene060840	MS.gene82451	0.800694	1.28E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82451.t1	MTR_5g025690	96.500	200	7	0	1	200	1	200	6.87e-138	384
MS.gene82451.t1	MTR_7g011010	78.641	206	34	2	1	200	1	202	1.86e-100	289
MS.gene82451.t1	MTR_4g127560	67.033	182	52	2	27	200	17	198	3.22e-74	222
MS.gene82451.t1	MTR_2g023310	82.353	51	9	0	83	133	47	97	1.01e-21	85.5
MS.gene82451.t1	MTR_4g082050	42.520	127	68	4	71	196	17	139	3.19e-21	85.5
MS.gene82451.t1	MTR_7g074240	55.385	65	29	0	131	195	106	170	2.65e-16	73.6
MS.gene82451.t1	MTR_2g098890	27.835	194	115	5	29	197	11	204	1.15e-13	67.0
MS.gene82451.t1	MTR_4g115170	44.595	74	41	0	124	197	145	218	3.00e-13	66.2
MS.gene82451.t1	MTR_4g067270	34.921	126	68	4	85	197	19	143	1.67e-12	62.8
MS.gene82451.t1	MTR_7g089760	47.541	61	32	0	136	196	82	142	2.44e-12	62.4
MS.gene82451.t1	MTR_3g109320	48.333	60	31	0	136	195	119	178	6.83e-12	61.6
MS.gene82451.t1	MTR_8g107110	30.405	148	68	5	65	197	32	159	8.58e-12	61.2
MS.gene82451.t1	MTR_3g067610	35.000	120	66	4	85	195	19	135	3.37e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82451.t1	AT5G39670	45.000	200	89	5	14	200	13	204	1.31e-43	145
MS.gene82451.t1	AT3G29000	60.185	108	41	1	89	196	85	190	6.90e-37	127
MS.gene82451.t1	AT4G20780	56.338	71	28	1	125	195	116	183	1.61e-16	74.3
MS.gene82451.t1	AT3G47480	56.061	66	28	1	131	196	115	179	3.55e-16	73.6
MS.gene82451.t1	AT5G44460	49.425	87	41	1	110	196	92	175	3.71e-16	73.2
MS.gene82451.t1	AT3G59440	29.474	190	105	5	29	197	7	188	1.37e-15	72.0
MS.gene82451.t1	AT2G43290	32.308	130	69	2	85	196	79	207	8.09e-15	70.5
MS.gene82451.t1	AT1G05990	47.692	65	34	0	133	197	80	144	6.22e-14	66.6
MS.gene82451.t1	AT3G07490	45.161	62	34	0	136	197	82	143	8.81e-13	63.5
MS.gene82451.t1	AT4G03290	46.875	64	34	0	133	196	82	145	1.42e-12	63.2
MS.gene82451.t1	AT4G12860	45.161	62	34	0	136	197	82	143	1.67e-11	60.1
MS.gene82451.t1	AT1G66410	49.254	67	32	1	129	195	46	110	7.53e-11	57.4
MS.gene82451.t1	AT1G66410	49.254	67	32	1	129	195	46	110	7.53e-11	57.4

Find 23 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAAAGAGTTATGTGTATTT+TGG	0.141163	5.2:+16337687	MS.gene82451:CDS
GAGAAAGTGGAGAGATTAAG+AGG	0.257266	5.2:+16337473	MS.gene82451:CDS
ACTTCATGTTCAACTTTGTT+TGG	0.288498	5.2:+16337298	MS.gene82451:CDS
TGAAACGGTGATGGTGAAAA+TGG	0.301570	5.2:+16337504	MS.gene82451:CDS
CTTCTCTGCCAACTTCGTTC+CGG	0.355688	5.2:+16337394	MS.gene82451:CDS
TGATGAGAATGAGCCAAGTT+TGG	0.401446	5.2:+16337597	MS.gene82451:CDS
ATCTTCACTTCCTCCAAACT+TGG	0.413419	5.2:-16337610	None:intergenic
CAAAGTAAAGGGAGAGAAGC+AGG	0.454619	5.2:+16337423	MS.gene82451:CDS
TCCCTTTACTTTGTAGTCAC+CGG	0.469829	5.2:-16337413	None:intergenic
TCCGGTGACTACAAAGTAAA+GGG	0.473575	5.2:+16337412	MS.gene82451:CDS
TGAAATCAATTCTTCCATCA+TGG	0.475727	5.2:-16337771	None:intergenic
CGGAACGAAGTTGGCAGAGA+AGG	0.482954	5.2:-16337393	None:intergenic
TCAAATGTTAGCAGAGAAAG+TGG	0.493623	5.2:+16337460	MS.gene82451:CDS
AAGTTTGGAGGAAGTGAAGA+TGG	0.496634	5.2:+16337612	MS.gene82451:CDS
AGAAAGTGGAGAGATTAAGA+GGG	0.503717	5.2:+16337474	MS.gene82451:CDS
TGAGAATGAGCCAAGTTTGG+AGG	0.510803	5.2:+16337600	MS.gene82451:CDS
AGATGGATTTATTGATGCAA+AGG	0.523959	5.2:+16337660	MS.gene82451:CDS
TTCCGGTGACTACAAAGTAA+AGG	0.525643	5.2:+16337411	MS.gene82451:CDS
TAAGAGGGATGAAGTTGAAA+CGG	0.526266	5.2:+16337489	MS.gene82451:CDS
ATTTGTTTGTCTTATTGAAG+AGG	0.533554	5.2:+16337243	MS.gene82451:CDS
TGTAGTCACCGGAACGAAGT+TGG	0.544456	5.2:-16337402	None:intergenic
GAAACGGTGATGGTGAAAAT+GGG	0.550238	5.2:+16337505	MS.gene82451:CDS
GGATGAAGTTGAAACGGTGA+TGG	0.648916	5.2:+16337495	MS.gene82451:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CACTGAATTTGTAAAAATTA+TGG	+	chr5.2:16337792-16337811	MS.gene82451:CDS	20.0%
!!!	AAAAGTTTTTCTCAACTTTT+TGG	+	chr5.2:16337365-16337384	MS.gene82451:CDS	20.0%
!	GATGAACTTGATGAAAAATA+TGG	+	chr5.2:16337550-16337569	MS.gene82451:CDS	25.0%
!!	ATTTGTTTGTCTTATTGAAG+AGG	+	chr5.2:16337243-16337262	MS.gene82451:CDS	25.0%
!!	CAAAGAGTTATGTGTATTTT+GGG	+	chr5.2:16337688-16337707	MS.gene82451:CDS	25.0%
!!!	AATTTTGATGAAAACCATGA+TGG	+	chr5.2:16337757-16337776	MS.gene82451:CDS	25.0%
	ACTTCATGTTCAACTTTGTT+TGG	+	chr5.2:16337298-16337317	MS.gene82451:CDS	30.0%
	TGAAATCAATTCTTCCATCA+TGG	-	chr5.2:16337774-16337793	None:intergenic	30.0%
!	AGATGGATTTATTGATGCAA+AGG	+	chr5.2:16337660-16337679	MS.gene82451:CDS	30.0%
!	GCAAAGAGTTATGTGTATTT+TGG	+	chr5.2:16337687-16337706	MS.gene82451:CDS	30.0%
!	GTTTTTGATGAGAACAAAGA+TGG	+	chr5.2:16337643-16337662	MS.gene82451:CDS	30.0%
!!!	TATGTGTATTTTGGGATTGA+AGG	+	chr5.2:16337696-16337715	MS.gene82451:CDS	30.0%
	AGAAAGTGGAGAGATTAAGA+GGG	+	chr5.2:16337474-16337493	MS.gene82451:CDS	35.0%
	TAAGAGGGATGAAGTTGAAA+CGG	+	chr5.2:16337489-16337508	MS.gene82451:CDS	35.0%
	TCAAATGTTAGCAGAGAAAG+TGG	+	chr5.2:16337460-16337479	MS.gene82451:CDS	35.0%
!!!	TGTATTTTGGGATTGAAGGA+AGG	+	chr5.2:16337700-16337719	MS.gene82451:CDS	35.0%
	AAGTTTGGAGGAAGTGAAGA+TGG	+	chr5.2:16337612-16337631	MS.gene82451:CDS	40.0%
	ATCTTCACTTCCTCCAAACT+TGG	-	chr5.2:16337613-16337632	None:intergenic	40.0%
	GAGAAAGTGGAGAGATTAAG+AGG	+	chr5.2:16337473-16337492	MS.gene82451:CDS	40.0%
	TCCCTTTACTTTGTAGTCAC+CGG	-	chr5.2:16337416-16337435	None:intergenic	40.0%
	TCCGGTGACTACAAAGTAAA+GGG	+	chr5.2:16337412-16337431	MS.gene82451:CDS	40.0%
	TGATGAGAATGAGCCAAGTT+TGG	+	chr5.2:16337597-16337616	MS.gene82451:CDS	40.0%
	TTCCGGTGACTACAAAGTAA+AGG	+	chr5.2:16337411-16337430	MS.gene82451:CDS	40.0%
!	GAAACGGTGATGGTGAAAAT+GGG	+	chr5.2:16337505-16337524	MS.gene82451:CDS	40.0%
!	TGAAACGGTGATGGTGAAAA+TGG	+	chr5.2:16337504-16337523	MS.gene82451:CDS	40.0%
	CAAAGTAAAGGGAGAGAAGC+AGG	+	chr5.2:16337423-16337442	MS.gene82451:CDS	45.0%
	GGATGAAGTTGAAACGGTGA+TGG	+	chr5.2:16337495-16337514	MS.gene82451:CDS	45.0%
	TGAGAATGAGCCAAGTTTGG+AGG	+	chr5.2:16337600-16337619	MS.gene82451:CDS	45.0%
	CTTCTCTGCCAACTTCGTTC+CGG	+	chr5.2:16337394-16337413	MS.gene82451:CDS	50.0%
	TGTAGTCACCGGAACGAAGT+TGG	-	chr5.2:16337405-16337424	None:intergenic	50.0%
	CGGAACGAAGTTGGCAGAGA+AGG	-	chr5.2:16337396-16337415	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	16337229	16337831	16337229	ID=MS.gene82451
chr5.2	mRNA	16337229	16337831	16337229	ID=MS.gene82451.t1;Parent=MS.gene82451
chr5.2	exon	16337229	16337831	16337229	ID=MS.gene82451.t1.exon1;Parent=MS.gene82451.t1
chr5.2	CDS	16337229	16337831	16337229	ID=cds.MS.gene82451.t1;Parent=MS.gene82451.t1

Gene Sequence

>MS.gene82451

ATGGTTTTTAGTCAATTTGTTTGTCTTATTGAAGAGGAGAAGACATCACAGAATGATGATAGCAACTCAACTTCATGTTCAACTTTGTTTGGTTTGATAGATTTTTTCATATATTCAACATTATTCATCAGAATACAAAAGTTTTTCTCAACTTTTTGGTTTTTCCTTCTCTGCCAACTTCGTTCCGGTGACTACAAAGTAAAGGGAGAGAAGCAGGTATCAGAAACTGAATCAAATGTTAGCAGAGAAAGTGGAGAGATTAAGAGGGATGAAGTTGAAACGGTGATGGTGAAAATGGGAATTTTTTGCAGCTCAGAAAGTGATGAACTTGATGAAAAATATGGTTCAAATGAACTTTCTGAATTGTTTGATGAGAATGAGCCAAGTTTGGAGGAAGTGAAGATGGCTTTTGATGTTTTTGATGAGAACAAAGATGGATTTATTGATGCAAAGGAATTGCAAAGAGTTATGTGTATTTTGGGATTGAAGGAAGGTTTAGAAGTTAAGAATTGTCAGAAGATGATCAAGAATTTTGATGAAAACCATGATGGAAGAATTGATTTCACTGAATTTGTAAAAATTATGGAAAACAGATTGTGTTGA

Protein sequence

>MS.gene82451.t1

MVFSQFVCLIEEEKTSQNDDSNSTSCSTLFGLIDFFIYSTLFIRIQKFFSTFWFFLLCQLRSGDYKVKGEKQVSETESNVSRESGEIKRDEVETVMVKMGIFCSSESDELDEKYGSNELSELFDENEPSLEEVKMAFDVFDENKDGFIDAKELQRVMCILGLKEGLEVKNCQKMIKNFDENHDGRIDFTEFVKIMENRLC