AlfalfaGEDB | MS.gene82497

M. sativa cultivar XinJiangDaYe / MS.gene82497

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82497.t1	XP_013453611.1	98.6	143	2	0	1	143	1	143	4.80E-69	270.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82497.t1	I3SJ84	98.6	143	2	0	1	143	1	143	3.5e-69	270.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049419	MS.gene82497	-0.801415	9.10E-49	-1.69E-46
MS.gene051452	MS.gene82497	0.810445	1.15E-50	-1.69E-46
MS.gene052954	MS.gene82497	0.811157	8.06E-51	-1.69E-46
MS.gene053006	MS.gene82497	0.816225	6.17E-52	-1.69E-46
MS.gene053008	MS.gene82497	0.801186	1.01E-48	-1.69E-46
MS.gene055623	MS.gene82497	0.823147	1.62E-53	-1.69E-46
MS.gene055701	MS.gene82497	0.811153	8.07E-51	-1.69E-46
MS.gene056699	MS.gene82497	0.809073	2.27E-50	-1.69E-46
MS.gene057208	MS.gene82497	0.829868	4.05E-55	-1.69E-46
MS.gene058783	MS.gene82497	0.800113	1.68E-48	-1.69E-46
MS.gene059952	MS.gene82497	0.808012	3.82E-50	-1.69E-46
MS.gene060527	MS.gene82497	0.801756	7.75E-49	-1.69E-46
MS.gene060935	MS.gene82497	0.821498	3.91E-53	-1.69E-46
MS.gene060937	MS.gene82497	0.809652	1.70E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82497.t1	MTR_5g025015	98.601	143	2	0	1	143	1	143	8.78e-99	280

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82497.t1	AT3G51500	50.000	138	47	5	1	138	1	116	5.79e-26	95.1

Find 44 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTGAAGTAGAGATGCTTTC+TGG	0.155423	5.2:+15680713	None:intergenic
TTCAGTCATCAAGTGTCTTT+TGG	0.232137	5.2:+15680624	None:intergenic
GAGGAAGATGGCTTTGTATT+AGG	0.253421	5.2:-15680493	MS.gene82497:CDS
AGAGGAGAAGATTGCATTGC+TGG	0.335131	5.2:-15680428	MS.gene82497:CDS
GAATCTGAATTGGAACCTTC+TGG	0.388596	5.2:+15680580	None:intergenic
GCTAAAAGAAGCTGAAGATC+AGG	0.403208	5.2:-15680464	MS.gene82497:CDS
GCTCCTCTTCCGTTGAAATC+AGG	0.411458	5.2:-15680658	MS.gene82497:CDS
TTGAAGTAGAGATGCTTTCT+GGG	0.412709	5.2:+15680714	None:intergenic
GTCGGGCAACGAATCTGAAT+TGG	0.413676	5.2:+15680570	None:intergenic
TCCTCTGACAAAGGAATCCT+TGG	0.414586	5.2:+15680775	None:intergenic
ATGCAATCTTCTCCTCTTCA+AGG	0.425654	5.2:+15680434	None:intergenic
GGAAGGCCTTGTTGATCCCT+TGG	0.442315	5.2:-15680389	MS.gene82497:CDS
GGAAATATTGTCAAGGGAGG+GGG	0.456451	5.2:-15680685	MS.gene82497:CDS
GAACCTTCTGGTCTCTGAAA+AGG	0.466094	5.2:+15680592	None:intergenic
TTTCCACCCGCAGAGGAGTC+GGG	0.472445	5.2:+15680553	None:intergenic
AATACTTCCGCCTCAATTGA+CGG	0.473041	5.2:+15680524	None:intergenic
CTCAATATCTCCTCTGACAA+AGG	0.476118	5.2:+15680766	None:intergenic
TGTCCTGATTTCAACGGAAG+AGG	0.492333	5.2:+15680655	None:intergenic
ATTTCCACCCGCAGAGGAGT+CGG	0.498474	5.2:+15680552	None:intergenic
GCTGGATCAACTTGTTGTAA+TGG	0.502973	5.2:-15680410	MS.gene82497:CDS
AGGAAATATTGTCAAGGGAG+GGG	0.510018	5.2:-15680686	MS.gene82497:CDS
TCGTTGCCCGACTCCTCTGC+GGG	0.538190	5.2:-15680559	MS.gene82497:CDS
GAGGCGGAAGTATTTGCAGC+TGG	0.549105	5.2:-15680515	MS.gene82497:CDS
TTGCCCGACTCCTCTGCGGG+TGG	0.564389	5.2:-15680556	MS.gene82497:CDS
ACACTATGAGCGAATCTCCA+AGG	0.573497	5.2:+15680372	None:intergenic
GAAATGAATACCGTCAATTG+AGG	0.576227	5.2:-15680534	MS.gene82497:CDS
TATTTGCAGCTGGAGGAAGA+TGG	0.578348	5.2:-15680505	MS.gene82497:CDS
TGCAATCTTCTCCTCTTCAA+GGG	0.582806	5.2:+15680435	None:intergenic
CCTTCTGGTCTCTGAAAAGG+TGG	0.583162	5.2:+15680595	None:intergenic
TTCGTTGCCCGACTCCTCTG+CGG	0.588076	5.2:-15680560	MS.gene82497:CDS
TCAGGTAAAGACCCTTGAAG+AGG	0.601213	5.2:-15680446	MS.gene82497:CDS
GGTGAGAAATGCAAGATCCA+AGG	0.602347	5.2:-15680792	None:intergenic
TCCAAGGATTCCTTTGTCAG+AGG	0.602608	5.2:-15680776	MS.gene82497:CDS
CACTATGAGCGAATCTCCAA+GGG	0.604058	5.2:+15680373	None:intergenic
CTGCAAGGAAATATTGTCAA+GGG	0.605688	5.2:-15680691	MS.gene82497:CDS
AAGGAAATATTGTCAAGGGA+GGG	0.608332	5.2:-15680687	MS.gene82497:CDS
GATCAACTTGTTGTAATGGA+AGG	0.608527	5.2:-15680406	MS.gene82497:CDS
ACTGCAAGGAAATATTGTCA+AGG	0.612756	5.2:-15680692	MS.gene82497:CDS
CAAGGAAATATTGTCAAGGG+AGG	0.627083	5.2:-15680688	MS.gene82497:CDS
GCATCTCTACTTCAACTGCA+AGG	0.630440	5.2:-15680706	MS.gene82497:CDS
ATGAATACCGTCAATTGAGG+CGG	0.653058	5.2:-15680531	MS.gene82497:CDS
GCGGAAGTATTTGCAGCTGG+AGG	0.666953	5.2:-15680512	MS.gene82497:CDS
GTATTCATTTCCACCCGCAG+AGG	0.686415	5.2:+15680546	None:intergenic
GAATCTCCAAGGGATCAACA+AGG	0.729425	5.2:+15680383	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GTTGAGTTTTTCTTCTTCTT+TGG	+	chr5.2:15680426-15680445	None:intergenic	30.0%
!	TTTTTGTGTCCTGATTTCAA+CGG	+	chr5.2:15680513-15680532	None:intergenic	30.0%
	AAGGAAATATTGTCAAGGGA+GGG	-	chr5.2:15680472-15680491	MS.gene82497:CDS	35.0%
	ACTGCAAGGAAATATTGTCA+AGG	-	chr5.2:15680467-15680486	MS.gene82497:CDS	35.0%
	CTGCAAGGAAATATTGTCAA+GGG	-	chr5.2:15680468-15680487	MS.gene82497:CDS	35.0%
	GAAATGAATACCGTCAATTG+AGG	-	chr5.2:15680625-15680644	MS.gene82497:CDS	35.0%
	TTGAAGTAGAGATGCTTTCT+GGG	+	chr5.2:15680448-15680467	None:intergenic	35.0%
!	TTCAGTCATCAAGTGTCTTT+TGG	+	chr5.2:15680538-15680557	None:intergenic	35.0%
!!	GATCAACTTGTTGTAATGGA+AGG	-	chr5.2:15680753-15680772	MS.gene82497:CDS	35.0%
	AATACTTCCGCCTCAATTGA+CGG	+	chr5.2:15680638-15680657	None:intergenic	40.0%
	AGGAAATATTGTCAAGGGAG+GGG	-	chr5.2:15680473-15680492	MS.gene82497:CDS	40.0%
	ATGAATACCGTCAATTGAGG+CGG	-	chr5.2:15680628-15680647	MS.gene82497:CDS	40.0%
	ATGCAATCTTCTCCTCTTCA+AGG	+	chr5.2:15680728-15680747	None:intergenic	40.0%
	CAAGGAAATATTGTCAAGGG+AGG	-	chr5.2:15680471-15680490	MS.gene82497:CDS	40.0%
	CTCAATATCTCCTCTGACAA+AGG	+	chr5.2:15680396-15680415	None:intergenic	40.0%
	GAATCTGAATTGGAACCTTC+TGG	+	chr5.2:15680582-15680601	None:intergenic	40.0%
	GCTAAAAGAAGCTGAAGATC+AGG	-	chr5.2:15680695-15680714	MS.gene82497:CDS	40.0%
	GTTGAAGTAGAGATGCTTTC+TGG	+	chr5.2:15680449-15680468	None:intergenic	40.0%
	TGCAATCTTCTCCTCTTCAA+GGG	+	chr5.2:15680727-15680746	None:intergenic	40.0%
!!	GAGGAAGATGGCTTTGTATT+AGG	-	chr5.2:15680666-15680685	MS.gene82497:CDS	40.0%
!!	GCTGGATCAACTTGTTGTAA+TGG	-	chr5.2:15680749-15680768	MS.gene82497:CDS	40.0%
	AGAGGAGAAGATTGCATTGC+TGG	-	chr5.2:15680731-15680750	MS.gene82497:CDS	45.0%
	GAATCTCCAAGGGATCAACA+AGG	+	chr5.2:15680779-15680798	None:intergenic	45.0%
	GCATCTCTACTTCAACTGCA+AGG	-	chr5.2:15680453-15680472	MS.gene82497:CDS	45.0%
	GGAAATATTGTCAAGGGAGG+GGG	-	chr5.2:15680474-15680493	MS.gene82497:CDS	45.0%
	TATTTGCAGCTGGAGGAAGA+TGG	-	chr5.2:15680654-15680673	MS.gene82497:CDS	45.0%
	TCAGGTAAAGACCCTTGAAG+AGG	-	chr5.2:15680713-15680732	MS.gene82497:CDS	45.0%
	TCCAAGGATTCCTTTGTCAG+AGG	-	chr5.2:15680383-15680402	MS.gene82497:CDS	45.0%
	TCCTCTGACAAAGGAATCCT+TGG	+	chr5.2:15680387-15680406	None:intergenic	45.0%
	TGTCCTGATTTCAACGGAAG+AGG	+	chr5.2:15680507-15680526	None:intergenic	45.0%
!	GAACCTTCTGGTCTCTGAAA+AGG	+	chr5.2:15680570-15680589	None:intergenic	45.0%
	GCTCCTCTTCCGTTGAAATC+AGG	-	chr5.2:15680501-15680520	MS.gene82497:CDS	50.0%
	GTATTCATTTCCACCCGCAG+AGG	+	chr5.2:15680616-15680635	None:intergenic	50.0%
	GTCGGGCAACGAATCTGAAT+TGG	+	chr5.2:15680592-15680611	None:intergenic	50.0%
!	CCACCTTTTCAGAGACCAGA+AGG	-	chr5.2:15680564-15680583	MS.gene82497:CDS	50.0%
!	CCTTCTGGTCTCTGAAAAGG+TGG	+	chr5.2:15680567-15680586	None:intergenic	50.0%
	ATTTCCACCCGCAGAGGAGT+CGG	+	chr5.2:15680610-15680629	None:intergenic	55.0%
	TATTGTCAAGGGAGGGGGAC+AGG	-	chr5.2:15680479-15680498	MS.gene82497:CDS	55.0%
!!	GAGGCGGAAGTATTTGCAGC+TGG	-	chr5.2:15680644-15680663	MS.gene82497:CDS	55.0%
!!	GCGGAAGTATTTGCAGCTGG+AGG	-	chr5.2:15680647-15680666	MS.gene82497:CDS	55.0%
!!	GGAAGGCCTTGTTGATCCCT+TGG	-	chr5.2:15680770-15680789	MS.gene82497:CDS	55.0%
	TTCGTTGCCCGACTCCTCTG+CGG	-	chr5.2:15680599-15680618	MS.gene82497:CDS	60.0%
	TTTCCACCCGCAGAGGAGTC+GGG	+	chr5.2:15680609-15680628	None:intergenic	60.0%
	TCGTTGCCCGACTCCTCTGC+GGG	-	chr5.2:15680600-15680619	MS.gene82497:CDS	65.0%
	TTGCCCGACTCCTCTGCGGG+TGG	-	chr5.2:15680603-15680622	MS.gene82497:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	15680375	15680806	15680375	ID=MS.gene82497
chr5.2	mRNA	15680375	15680806	15680375	ID=MS.gene82497.t1;Parent=MS.gene82497
chr5.2	exon	15680375	15680806	15680375	ID=MS.gene82497.t1.exon1;Parent=MS.gene82497.t1
chr5.2	CDS	15680375	15680806	15680375	ID=cds.MS.gene82497.t1;Parent=MS.gene82497.t1

Gene Sequence

>MS.gene82497

ATGCAAGATCCAAGGATTCCTTTGTCAGAGGAGATATTGAGTATTAAACCAAAGAAGAAGAAAAACTCAACCCAGAAAGCATCTCTACTTCAACTGCAAGGAAATATTGTCAAGGGAGGGGGACAGGCTCCTCTTCCGTTGAAATCAGGACACAAAAATTCCAAAAGACACTTGATGACTGAAGTCTCGCCACCTTTTCAGAGACCAGAAGGTTCCAATTCAGATTCGTTGCCCGACTCCTCTGCGGGTGGAAATGAATACCGTCAATTGAGGCGGAAGTATTTGCAGCTGGAGGAAGATGGCTTTGTATTAGGAAAAGAGCTAAAAGAAGCTGAAGATCAGGTAAAGACCCTTGAAGAGGAGAAGATTGCATTGCTGGATCAACTTGTTGTAATGGAAGGCCTTGTTGATCCCTTGGAGATTCGCTCATAG

Protein sequence

>MS.gene82497.t1

MQDPRIPLSEEILSIKPKKKKNSTQKASLLQLQGNIVKGGGQAPLPLKSGHKNSKRHLMTEVSPPFQRPEGSNSDSLPDSSAGGNEYRQLRRKYLQLEEDGFVLGKELKEAEDQVKTLEEEKIALLDQLVVMEGLVDPLEIRS