AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85177

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85177.t1 MTR_2g097540 94.670 394 21 0 1 394 50 443 0.0 769
MS.gene85177.t1 MTR_2g100520 94.162 394 23 0 1 394 54 447 0.0 767
MS.gene85177.t1 MTR_2g097530 83.756 394 64 0 1 394 54 447 0.0 677
MS.gene85177.t1 MTR_2g100510 83.503 394 65 0 1 394 54 447 0.0 677
MS.gene85177.t1 MTR_2g100560 83.249 394 66 0 1 394 59 452 0.0 675
MS.gene85177.t1 MTR_8g015510 72.796 397 105 3 1 394 51 447 0.0 582
MS.gene85177.t1 MTR_8g015510 70.642 327 93 3 71 394 1 327 2.16e-161 456
MS.gene85177.t1 MTR_3g088590 55.496 373 166 0 4 376 100 472 5.53e-151 436
MS.gene85177.t1 MTR_8g062790 56.267 375 162 1 1 375 52 424 1.47e-149 430
MS.gene85177.t1 MTR_8g075310 54.301 372 153 2 1 372 59 413 1.66e-137 399
MS.gene85177.t1 MTR_3g093030 48.936 376 192 0 1 376 40 415 7.93e-132 385
MS.gene85177.t1 MTR_2g100550 97.273 110 3 0 1 110 50 159 2.54e-68 227
MS.gene85177.t1 MTR_8g063060 42.484 306 144 7 69 371 58 334 6.85e-68 219
MS.gene85177.t1 MTR_1g028070 65.789 114 39 0 102 215 14 127 1.01e-46 156
MS.gene85177.t1 MTR_3g077040 51.493 134 41 2 84 217 1 110 2.17e-33 121
MS.gene85177.t1 MTR_2g100530 91.071 56 5 0 339 394 14 69 2.03e-32 117
MS.gene85177.t1 MTR_3g093040 43.564 101 47 1 84 174 1 101 8.72e-19 86.7
MS.gene85177.t1 MTR_8g062090 48.246 114 21 3 110 217 29 110 6.84e-15 70.9
MS.gene85177.t1 MTR_1205s0010 27.976 168 115 3 198 364 1 163 1.54e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85177.t1 AT1G51760 69.543 394 119 1 1 394 48 440 0.0 570
MS.gene85177.t1 AT1G51780 67.287 376 123 0 1 376 48 423 0.0 530
MS.gene85177.t1 AT5G56660 61.929 394 145 4 1 394 51 439 4.17e-175 495
MS.gene85177.t1 AT5G56650 61.675 394 146 4 1 394 50 438 3.58e-174 493
MS.gene85177.t1 AT5G56650 70.881 261 74 1 1 261 50 308 7.55e-134 385
MS.gene85177.t1 AT3G02875 51.459 377 181 2 1 376 52 427 6.75e-133 388
MS.gene85177.t1 AT5G54140 49.485 388 195 1 1 387 41 428 9.70e-132 385
MS.gene85177.t1 AT1G44350 52.279 373 173 1 4 376 92 459 2.87e-131 385
MS.gene85177.t1 AT3G02875 57.308 260 111 0 1 260 52 311 8.41e-100 300
MS.gene85177.t1 AT3G02875 58.986 217 89 0 1 217 52 268 3.05e-85 262

Find 99 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAATGTCTCTGCCATATT+TGG 0.136247 2.4:+3912993 MS.gene85177:CDS
CACATTCGAAGCCGCCAAAA+TGG 0.224265 2.4:-3913153 None:intergenic
ACATGCTATTGACCCCATTT+TGG 0.230672 2.4:+3913139 MS.gene85177:CDS
GTTCATGTTGCTATGCTTCT+TGG 0.234809 2.4:+3912435 MS.gene85177:CDS
ATCTTCGTTGACTTTGAAAT+AGG 0.251089 2.4:-3914077 None:intergenic
CTCGGCGTTGATAAAGTTAA+AGG 0.255745 2.4:+3913935 MS.gene85177:CDS
ACTGCAACTGGATGTTTATA+TGG 0.283261 2.4:-3912151 None:intergenic
CCACCAATTGTGACGGAGTC+TGG 0.290981 2.4:-3913549 None:intergenic
GTTGCAGTGACAGGTGTTAT+TGG 0.296181 2.4:+3912166 MS.gene85177:CDS
TCCAACATGCATTCCAAATA+TGG 0.297332 2.4:-3913006 None:intergenic
TTGATTGGATGGTTGATATA+AGG 0.308095 2.4:+3912041 None:intergenic
AGAACAAAACTGGATGAATT+AGG 0.309103 2.4:+3912124 MS.gene85177:CDS
TCTTCGTTGACTTTGAAATA+GGG 0.331390 2.4:-3914076 None:intergenic
CCACCACCTCCTTCTTCTGC+TGG 0.334818 2.4:-3912939 None:intergenic
GTTGGACCTAACATACCTTT+AGG 0.355719 2.4:+3913023 MS.gene85177:CDS
AATTCCTCATAACCAAGTTC+AGG 0.364088 2.4:-3912082 None:intergenic
TAAGCTTATAAGAACAAAAC+TGG 0.365890 2.4:+3912114 MS.gene85177:CDS
ATAACACCTGTCACTGCAAC+TGG 0.378254 2.4:-3912163 None:intergenic
ACATTCGAAGCCGCCAAAAT+GGG 0.396292 2.4:-3913152 None:intergenic
AACGAAGATGTACTTCCTTA+TGG 0.401836 2.4:+3914091 MS.gene85177:CDS
CAGAAGGTACCAGCGGTAGA+TGG 0.406538 2.4:+3914163 MS.gene85177:CDS
CCACATGCATGCATCTTTCC+AGG 0.410681 2.4:-3912405 None:intergenic
AGGTCAGGTCCTATGCTTGC+AGG 0.414114 2.4:+3913059 MS.gene85177:CDS
ACTTCACCTAAAGGTATGTT+AGG 0.418812 2.4:-3913029 None:intergenic
TCGGTCTTGTAGGTTATCAT+TGG 0.420486 2.4:+3913788 MS.gene85177:intron
GTTATTGGTTACATAGGAAC+TGG 0.430657 2.4:+3912181 MS.gene85177:CDS
ATGTCACTGTTGAACGTCTT+AGG 0.432290 2.4:+3914041 MS.gene85177:CDS
TTCTATCAAGAGGCTATGCC+TGG 0.441380 2.4:+3913992 MS.gene85177:CDS
CCGACGATGAAGACGTATCC+AGG 0.444921 2.4:-3914010 None:intergenic
AAGCCATCATTGTTTATAGT+TGG 0.445942 2.4:-3913878 None:intergenic
GGTCCTATGCTTGCAGGAGG+TGG 0.448207 2.4:+3913065 MS.gene85177:CDS
ATTCCAGACTCCGTCACAAT+TGG 0.451819 2.4:+3913546 MS.gene85177:CDS
CGCTTATTACAGCTTTAAAG+AGG 0.458236 2.4:-3913088 None:intergenic
ACCTTAGGAATTGGTGGAGT+GGG 0.463973 2.4:+3912365 MS.gene85177:intron
CCATCATTGTTTATAGTTGG+AGG 0.474622 2.4:-3913875 None:intergenic
ACGTGAAGTGCAGCGCCATA+AGG 0.477916 2.4:-3914106 None:intergenic
AGTGTTGCTGGGAGTTTGCT+CGG 0.481679 2.4:+3913917 MS.gene85177:CDS
CTTTAGGTGAAGTGGCATCT+AGG 0.486875 2.4:+3913039 MS.gene85177:CDS
CCAGCAGAAGAAGGAGGTGG+TGG 0.487742 2.4:+3912939 MS.gene85177:CDS
GAATCCTGAACTTGGTTATG+AGG 0.491210 2.4:+3912078 MS.gene85177:CDS
CTAGATGCCACTTCACCTAA+AGG 0.496058 2.4:-3913038 None:intergenic
TGGTTAACCTTAGGAATTGG+TGG 0.501988 2.4:+3912359 MS.gene85177:intron
CTGCAACTGGATGTTTATAT+GGG 0.502686 2.4:-3912150 None:intergenic
TAAAGGCCATCATCTATTGA+TGG 0.507495 2.4:+3913952 MS.gene85177:CDS
TGGTACCTTCTGATTTAACT+TGG 0.508478 2.4:-3914152 None:intergenic
ATTCATGAGAATCCTGAACT+TGG 0.516390 2.4:+3912070 MS.gene85177:CDS
AAACATCCAGTTGCAGTGAC+AGG 0.518282 2.4:+3912157 MS.gene85177:CDS
CCAGACTCCGTCACAATTGG+TGG 0.521978 2.4:+3913549 MS.gene85177:CDS
ACAGGTGTTATTGGTTACAT+AGG 0.522130 2.4:+3912175 MS.gene85177:CDS
GCCATATTTGGAATGCATGT+TGG 0.526392 2.4:+3913005 MS.gene85177:CDS
GGTGAAGTGGCATCTAGGTC+AGG 0.527865 2.4:+3913044 MS.gene85177:CDS
CTGATCCCATCAATAGATGA+TGG 0.528032 2.4:-3913958 None:intergenic
ATTGTTTATAGTTGGAGGGC+AGG 0.533896 2.4:-3913870 None:intergenic
CATCATTGTTTATAGTTGGA+GGG 0.535379 2.4:-3913874 None:intergenic
CATGGATGCTCTTCTCATGC+AGG 0.545031 2.4:+3912234 MS.gene85177:CDS
AGGCCACCTCCTGCAAGCAT+AGG 0.551306 2.4:-3913068 None:intergenic
TAACATACCTTTAGGTGAAG+TGG 0.552311 2.4:+3913031 MS.gene85177:CDS
GCTCTAAGAGCGACAAAAGG+AGG 0.552904 2.4:-3912208 None:intergenic
ATACGTCTTCATCGTCGGAA+TGG 0.554205 2.4:+3914015 MS.gene85177:CDS
CCTGGATACGTCTTCATCGT+CGG 0.554573 2.4:+3914010 MS.gene85177:CDS
CCTCCAACTATAAACAATGA+TGG 0.555815 2.4:+3913875 MS.gene85177:CDS
CAGCAGAAGAAGGAGGTGGT+GGG 0.556101 2.4:+3912940 MS.gene85177:CDS
GGCTGATCCCCTTGACACCC+AGG 0.556701 2.4:+3913208 MS.gene85177:CDS
ATTGGATGGTTGATATAAGG+AGG 0.557920 2.4:+3912044 None:intergenic
AGACTTTGCATTCTATCAAG+AGG 0.559534 2.4:+3913982 MS.gene85177:CDS
AAAGGCCATCATCTATTGAT+GGG 0.563107 2.4:+3913953 MS.gene85177:CDS
GGTAACAGACCTGCTCAATA+CGG 0.565635 2.4:-3913617 None:intergenic
CATGTTGAGGATTGGCTGCA+TGG 0.565648 2.4:-3913121 None:intergenic
GTCAATAGCATGTTGAGGAT+TGG 0.566408 2.4:-3913129 None:intergenic
AGCAGAAGAAGGAGGTGGTG+GGG 0.566893 2.4:+3912941 MS.gene85177:CDS
TGTCGCTCTTAGAGCTGACA+TGG 0.574773 2.4:+3912216 MS.gene85177:CDS
CTCTTTAAAGCTGTAATAAG+CGG 0.578968 2.4:+3913089 MS.gene85177:CDS
AGGACGGTATCTTGTCATCA+AGG 0.582123 2.4:-3913850 None:intergenic
TCCTCAAAGGTTGTGACTGT+AGG 0.582654 2.4:+3913492 MS.gene85177:intron
TCAGCTCTAAGAGCGACAAA+AGG 0.584462 2.4:-3912211 None:intergenic
TAAAGCTGTAATAAGCGGCA+AGG 0.586608 2.4:+3913094 MS.gene85177:CDS
CAACCAGCAGAAGAAGGAGG+TGG 0.586751 2.4:+3912936 MS.gene85177:CDS
TCTTGCCAAGTTAAATCAGA+AGG 0.589153 2.4:+3914147 MS.gene85177:CDS
ACTAAGACACCGTATTGAGC+AGG 0.590097 2.4:+3913608 MS.gene85177:CDS
CTGTTGAACGTCTTAGGTCA+TGG 0.595588 2.4:+3914047 MS.gene85177:CDS
GAGCATAAGAGTAAAGTGCC+TGG 0.597679 2.4:+3912387 MS.gene85177:CDS
ACAACATATCGTTTCTCGCG+AGG 0.602436 2.4:+3913187 MS.gene85177:CDS
GTAGGAAAGATTCAAGGAGG+TGG 0.603869 2.4:+3913510 MS.gene85177:CDS
GTGACTGTAGGAAAGATTCA+AGG 0.604494 2.4:+3913504 MS.gene85177:CDS
AACCTTAGGAATTGGTGGAG+TGG 0.609555 2.4:+3912364 MS.gene85177:intron
TTTATAGTTGGAGGGCAGGA+CGG 0.612350 2.4:-3913866 None:intergenic
TCAGGTCCTATGCTTGCAGG+AGG 0.624384 2.4:+3913062 MS.gene85177:CDS
CATTCGAAGCCGCCAAAATG+GGG 0.630255 2.4:-3913151 None:intergenic
GCTGTAATAAGCGGCAAGGG+AGG 0.633306 2.4:+3913098 MS.gene85177:CDS
AAAGCTGTAATAAGCGGCAA+GGG 0.635247 2.4:+3913095 MS.gene85177:CDS
TTTCAACCAGCAGAAGAAGG+AGG 0.636316 2.4:+3912933 MS.gene85177:CDS
TTCACGTCTCATTAGCGACT+AGG 0.637245 2.4:+3914122 MS.gene85177:CDS
ATGGGGTCAATAGCATGTTG+AGG 0.637295 2.4:-3913134 None:intergenic
GAAGGTGCCACCAATTGTGA+CGG 0.642404 2.4:-3913556 None:intergenic
TGGTGCTGCTAAGATCCTCA+AGG 0.643332 2.4:+3912455 MS.gene85177:CDS
GTTAAATCAGAAGGTACCAG+CGG 0.706610 2.4:+3914156 MS.gene85177:CDS
CCTGGAAAGATGCATGCATG+TGG 0.716940 2.4:+3912405 MS.gene85177:CDS
TGCGTTGCATCTATGCACAG+CGG 0.748702 2.4:-3913816 None:intergenic
ACTGTAGGAAAGATTCAAGG+AGG 0.754125 2.4:+3913507 MS.gene85177:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAATAAAGTTGAAA+AGG + chr2.4:3912590-3912609 MS.gene85177:intron 10.0%
!!! AATTTTAAGCTTTAATTTTA+AGG - chr2.4:3912800-3912819 None:intergenic 10.0%
!! ATAAAATCCATGATAATATA+TGG - chr2.4:3912297-3912316 None:intergenic 15.0%
!!! ATTATCATGGATTTTATATT+AGG + chr2.4:3912300-3912319 MS.gene85177:intron 15.0%
!!! ATTATTTTCAATTACTTGTG+TGG + chr2.4:3912555-3912574 MS.gene85177:intron 20.0%
!!! ATTCCTTATTTTTTGGTAAA+GGG + chr2.4:3912894-3912913 MS.gene85177:intron 20.0%
!!! TGATATCATTCCTTATTTTT+TGG + chr2.4:3912887-3912906 MS.gene85177:intron 20.0%
!!! TTAAAAGCTTTGTATTCATT+TGG + chr2.4:3913263-3913282 MS.gene85177:intron 20.0%
!!! TTTTCAACCATATATTATCA+TGG + chr2.4:3912287-3912306 MS.gene85177:intron 20.0%
! AAAATAAAGTTGAAAAGGTG+TGG + chr2.4:3912595-3912614 MS.gene85177:intron 25.0%
! TAAAAAATTGCGAATGAATC+CGG + chr2.4:3912691-3912710 MS.gene85177:intron 25.0%
! TAACCAAAAAAAATCATCAG+TGG - chr2.4:3912345-3912364 None:intergenic 25.0%
! TAAGCTTATAAGAACAAAAC+TGG + chr2.4:3912114-3912133 MS.gene85177:CDS 25.0%
! TCCTAAAATAATATCCTCAA+AGG + chr2.4:3913479-3913498 MS.gene85177:intron 25.0%
! TGATATCACATATCCATAAA+AGG - chr2.4:3912875-3912894 None:intergenic 25.0%
! TTGTTGATATTCGAATTGTT+AGG + chr2.4:3913703-3913722 MS.gene85177:intron 25.0%
!! TTTTACAATCACAATTCACA+AGG - chr2.4:3912676-3912695 None:intergenic 25.0%
!! TTTTGTTCTTATAAGCTTAC+TGG - chr2.4:3912113-3912132 None:intergenic 25.0%
!!! ACATTTTTTTCTGAGTGTTT+TGG + chr2.4:3912264-3912283 MS.gene85177:intron 25.0%
!!! ACCTTTGAGGATATTATTTT+AGG - chr2.4:3913483-3913502 None:intergenic 25.0%
!!! ATTCCACTGATGATTTTTTT+TGG + chr2.4:3912339-3912358 MS.gene85177:intron 25.0%
!!! CATTCCTTATTTTTTGGTAA+AGG + chr2.4:3912893-3912912 MS.gene85177:intron 25.0%
!!! GATTTTTTTTGGTTAACCTT+AGG + chr2.4:3912350-3912369 MS.gene85177:intron 25.0%
!!! TTATTTTTTGGTAAAGGGAA+CGG + chr2.4:3912899-3912918 MS.gene85177:intron 25.0%
AAGCCATCATTGTTTATAGT+TGG - chr2.4:3913881-3913900 None:intergenic 30.0%
AGAACAAAACTGGATGAATT+AGG + chr2.4:3912124-3912143 MS.gene85177:CDS 30.0%
ATAAGACACAATTCATGATG+TGG - chr2.4:3913677-3913696 None:intergenic 30.0%
ATCACATATCCATAAAAGGA+TGG - chr2.4:3912871-3912890 None:intergenic 30.0%
ATCTCAGTGATAACTAAAAC+AGG - chr2.4:3913746-3913765 None:intergenic 30.0%
ATGAAAAACAGTTACAGGTA+TGG + chr2.4:3912481-3912500 MS.gene85177:intron 30.0%
CATCATTGTTTATAGTTGGA+GGG - chr2.4:3913877-3913896 None:intergenic 30.0%
CATTGTGAATATGTTCTTCT+TGG - chr2.4:3913641-3913660 None:intergenic 30.0%
CTGTTTGAGGAAAAAAATGT+TGG + chr2.4:3913386-3913405 MS.gene85177:intron 30.0%
GAATCATACAATACTCATGT+TGG + chr2.4:3913335-3913354 MS.gene85177:intron 30.0%
GTTCCCTTTACCAAAAAATA+AGG - chr2.4:3912900-3912919 None:intergenic 30.0%
TTGAATACGATGAAAATCTG+AGG + chr2.4:3913445-3913464 MS.gene85177:intron 30.0%
TTGTAGTTTCTGCAATTCAA+TGG - chr2.4:3912643-3912662 None:intergenic 30.0%
! CTTTTCTTTCCATCCTTTTA+TGG + chr2.4:3912859-3912878 MS.gene85177:intron 30.0%
!! ATCTTCGTTGACTTTGAAAT+AGG - chr2.4:3914080-3914099 None:intergenic 30.0%
!! CTCTTTAAAGCTGTAATAAG+CGG + chr2.4:3913089-3913108 MS.gene85177:CDS 30.0%
!! TCCAGCATCTAAAATTTTCT+TGG - chr2.4:3912967-3912986 None:intergenic 30.0%
!! TCTTCGTTGACTTTGAAATA+GGG - chr2.4:3914079-3914098 None:intergenic 30.0%
!! TTTTGGTTAACCTTAGGAAT+TGG + chr2.4:3912356-3912375 MS.gene85177:intron 30.0%
!!! ACTATTTTCAAAGTGTTGCT+GGG + chr2.4:3913906-3913925 MS.gene85177:CDS 30.0%
AAAGGCCATCATCTATTGAT+GGG + chr2.4:3913953-3913972 MS.gene85177:CDS 35.0%
AACGAAGATGTACTTCCTTA+TGG + chr2.4:3914091-3914110 MS.gene85177:CDS 35.0%
AATTCCTCATAACCAAGTTC+AGG - chr2.4:3912085-3912104 None:intergenic 35.0%
ACTGCAACTGGATGTTTATA+TGG - chr2.4:3912154-3912173 None:intergenic 35.0%
AGACTTTGCATTCTATCAAG+AGG + chr2.4:3913982-3914001 MS.gene85177:CDS 35.0%
AGCCAAAATATTTCGCAAGA+TGG - chr2.4:3913368-3913387 None:intergenic 35.0%
AGCTTTCTCTCAAAAATGCT+CGG - chr2.4:3913581-3913600 None:intergenic 35.0%
ATATGCACTATGCAGTGTTA+AGG - chr2.4:3912760-3912779 None:intergenic 35.0%
ATTCATGAGAATCCTGAACT+TGG + chr2.4:3912070-3912089 MS.gene85177:CDS 35.0%
CCATCATTGTTTATAGTTGG+AGG - chr2.4:3913878-3913897 None:intergenic 35.0%
CCTCCAACTATAAACAATGA+TGG + chr2.4:3913875-3913894 MS.gene85177:CDS 35.0%
CTGCAACTGGATGTTTATAT+GGG - chr2.4:3912153-3912172 None:intergenic 35.0%
GAAAATGTCTCTGCCATATT+TGG + chr2.4:3912993-3913012 MS.gene85177:CDS 35.0%
GGAACATGAAAAACAGTTAC+AGG + chr2.4:3912476-3912495 MS.gene85177:CDS 35.0%
TAAAGGCCATCATCTATTGA+TGG + chr2.4:3913952-3913971 MS.gene85177:CDS 35.0%
TAACATACCTTTAGGTGAAG+TGG + chr2.4:3913031-3913050 MS.gene85177:CDS 35.0%
TCCAACATGCATTCCAAATA+TGG - chr2.4:3913009-3913028 None:intergenic 35.0%
TCTTGCCAAGTTAAATCAGA+AGG + chr2.4:3914147-3914166 MS.gene85177:CDS 35.0%
TGGTACCTTCTGATTTAACT+TGG - chr2.4:3914155-3914174 None:intergenic 35.0%
! ACTTCACCTAAAGGTATGTT+AGG - chr2.4:3913032-3913051 None:intergenic 35.0%
! ATGAGTTGCAAGTTTTTACC+TGG - chr2.4:3913229-3913248 None:intergenic 35.0%
! CACCATCTTGCGAAATATTT+TGG + chr2.4:3913363-3913382 MS.gene85177:intron 35.0%
! CATGAGTATTGTATGATTCG+TGG - chr2.4:3913334-3913353 None:intergenic 35.0%
! GAAAGAAAAGTATAATGCGC+AGG - chr2.4:3912849-3912868 None:intergenic 35.0%
! GCCAAGAAAATTTTAGATGC+TGG + chr2.4:3912963-3912982 MS.gene85177:CDS 35.0%
! GTTATTGGTTACATAGGAAC+TGG + chr2.4:3912181-3912200 MS.gene85177:CDS 35.0%
! TATGGCAGAGACATTTTCTA+AGG - chr2.4:3912991-3913010 None:intergenic 35.0%
! TGAGTTGCAAGTTTTTACCT+GGG - chr2.4:3913228-3913247 None:intergenic 35.0%
!! ACAGGTGTTATTGGTTACAT+AGG + chr2.4:3912175-3912194 MS.gene85177:CDS 35.0%
!! ACTGTTTTTCATGTTCCTTG+AGG - chr2.4:3912473-3912492 None:intergenic 35.0%
!! CGCTTATTACAGCTTTAAAG+AGG - chr2.4:3913091-3913110 None:intergenic 35.0%
!!! CGAAATATTTTGGCTGTTTG+AGG + chr2.4:3913373-3913392 MS.gene85177:intron 35.0%
!!! GACTATTTTCAAAGTGTTGC+TGG + chr2.4:3913905-3913924 MS.gene85177:CDS 35.0%
AAAGCTGTAATAAGCGGCAA+GGG + chr2.4:3913095-3913114 MS.gene85177:CDS 40.0%
ACTGTAGGAAAGATTCAAGG+AGG + chr2.4:3913507-3913526 MS.gene85177:CDS 40.0%
ATGTCACTGTTGAACGTCTT+AGG + chr2.4:3914041-3914060 MS.gene85177:CDS 40.0%
ATTGTTTATAGTTGGAGGGC+AGG - chr2.4:3913873-3913892 None:intergenic 40.0%
CAATTGGGATGCTATAGTTG+CGG + chr2.4:3912722-3912741 MS.gene85177:intron 40.0%
CTGATCCCATCAATAGATGA+TGG - chr2.4:3913961-3913980 None:intergenic 40.0%
GAATCCTGAACTTGGTTATG+AGG + chr2.4:3912078-3912097 MS.gene85177:CDS 40.0%
GTCAATAGCATGTTGAGGAT+TGG - chr2.4:3913132-3913151 None:intergenic 40.0%
GTGACTGTAGGAAAGATTCA+AGG + chr2.4:3913504-3913523 MS.gene85177:CDS 40.0%
GTTCATGTTGCTATGCTTCT+TGG + chr2.4:3912435-3912454 MS.gene85177:CDS 40.0%
GTTGGACCTAACATACCTTT+AGG + chr2.4:3913023-3913042 MS.gene85177:CDS 40.0%
TAAAGCTGTAATAAGCGGCA+AGG + chr2.4:3913094-3913113 MS.gene85177:CDS 40.0%
TGGTTAACCTTAGGAATTGG+TGG + chr2.4:3912359-3912378 MS.gene85177:intron 40.0%
TTCTCTCAAAAATGCTCGGA+AGG - chr2.4:3913577-3913596 None:intergenic 40.0%
! AAGTTTTTACCTGGGTGTCA+AGG - chr2.4:3913220-3913239 None:intergenic 40.0%
! ACATGCTATTGACCCCATTT+TGG + chr2.4:3913139-3913158 MS.gene85177:CDS 40.0%
! AGTTTTTACCTGGGTGTCAA+GGG - chr2.4:3913219-3913238 None:intergenic 40.0%
! GCCATATTTGGAATGCATGT+TGG + chr2.4:3913005-3913024 MS.gene85177:CDS 40.0%
! TCGGTCTTGTAGGTTATCAT+TGG + chr2.4:3913788-3913807 MS.gene85177:intron 40.0%
! TGATATTTTCCATCTACCGC+TGG - chr2.4:3914175-3914194 None:intergenic 40.0%
!! CTCGGCGTTGATAAAGTTAA+AGG + chr2.4:3913935-3913954 MS.gene85177:CDS 40.0%
!! GTTAAATCAGAAGGTACCAG+CGG + chr2.4:3914156-3914175 MS.gene85177:CDS 40.0%
!! GTTTTTCAACCAGCAGAAGA+AGG + chr2.4:3912930-3912949 MS.gene85177:CDS 40.0%
AAACATCCAGTTGCAGTGAC+AGG + chr2.4:3912157-3912176 MS.gene85177:CDS 45.0%
AAAGAGACTTCGCGTGTAAC+AGG - chr2.4:3913769-3913788 None:intergenic 45.0%
AACCTTAGGAATTGGTGGAG+TGG + chr2.4:3912364-3912383 MS.gene85177:intron 45.0%
AATCCGGCTTACGTGCAATT+GGG + chr2.4:3912707-3912726 MS.gene85177:intron 45.0%
ACAACATATCGTTTCTCGCG+AGG + chr2.4:3913187-3913206 MS.gene85177:CDS 45.0%
ACATTCGAAGCCGCCAAAAT+GGG - chr2.4:3913155-3913174 None:intergenic 45.0%
ACCTTAGGAATTGGTGGAGT+GGG + chr2.4:3912365-3912384 MS.gene85177:intron 45.0%
ACTAAGACACCGTATTGAGC+AGG + chr2.4:3913608-3913627 MS.gene85177:CDS 45.0%
AGGACGGTATCTTGTCATCA+AGG - chr2.4:3913853-3913872 None:intergenic 45.0%
ATAACACCTGTCACTGCAAC+TGG - chr2.4:3912166-3912185 None:intergenic 45.0%
ATAATGCGCAGGAGTCATCT+TGG - chr2.4:3912838-3912857 None:intergenic 45.0%
ATACGTCTTCATCGTCGGAA+TGG + chr2.4:3914015-3914034 MS.gene85177:CDS 45.0%
ATGGGGTCAATAGCATGTTG+AGG - chr2.4:3913137-3913156 None:intergenic 45.0%
ATTCCAGACTCCGTCACAAT+TGG + chr2.4:3913546-3913565 MS.gene85177:CDS 45.0%
CTGCAATTCAATGGCATGCT+TGG - chr2.4:3912634-3912653 None:intergenic 45.0%
CTGTTGAACGTCTTAGGTCA+TGG + chr2.4:3914047-3914066 MS.gene85177:CDS 45.0%
CTTTAGGTGAAGTGGCATCT+AGG + chr2.4:3913039-3913058 MS.gene85177:CDS 45.0%
GGTAACAGACCTGCTCAATA+CGG - chr2.4:3913620-3913639 None:intergenic 45.0%
GTAGGAAAGATTCAAGGAGG+TGG + chr2.4:3913510-3913529 MS.gene85177:CDS 45.0%
GTTACACGCGAAGTCTCTTT+CGG + chr2.4:3913769-3913788 MS.gene85177:intron 45.0%
TCCTACAGTCACAACCTTTG+AGG - chr2.4:3913496-3913515 None:intergenic 45.0%
TCCTCAAAGGTTGTGACTGT+AGG + chr2.4:3913492-3913511 MS.gene85177:intron 45.0%
TTCACGTCTCATTAGCGACT+AGG + chr2.4:3914122-3914141 MS.gene85177:CDS 45.0%
TTCTATCAAGAGGCTATGCC+TGG + chr2.4:3913992-3914011 MS.gene85177:CDS 45.0%
TTTATAGTTGGAGGGCAGGA+CGG - chr2.4:3913869-3913888 None:intergenic 45.0%
TTTCAACCAGCAGAAGAAGG+AGG + chr2.4:3912933-3912952 MS.gene85177:CDS 45.0%
! CTAGATGCCACTTCACCTAA+AGG - chr2.4:3913041-3913060 None:intergenic 45.0%
! GAAGTCTCTTTCGGTCTTGT+AGG + chr2.4:3913778-3913797 MS.gene85177:intron 45.0%
! GAGCATAAGAGTAAAGTGCC+TGG + chr2.4:3912387-3912406 MS.gene85177:CDS 45.0%
! GTTGCAGTGACAGGTGTTAT+TGG + chr2.4:3912166-3912185 MS.gene85177:CDS 45.0%
! GTTTTTACCTGGGTGTCAAG+GGG - chr2.4:3913218-3913237 None:intergenic 45.0%
! TGCTATTGACCCCATTTTGG+CGG + chr2.4:3913142-3913161 MS.gene85177:CDS 45.0%
!! TCAGCTCTAAGAGCGACAAA+AGG - chr2.4:3912214-3912233 None:intergenic 45.0%
CACATTCGAAGCCGCCAAAA+TGG - chr2.4:3913156-3913175 None:intergenic 50.0%
CATCCCAATTGCACGTAAGC+CGG - chr2.4:3912713-3912732 None:intergenic 50.0%
CATGGATGCTCTTCTCATGC+AGG + chr2.4:3912234-3912253 MS.gene85177:CDS 50.0%
CATTCGAAGCCGCCAAAATG+GGG - chr2.4:3913154-3913173 None:intergenic 50.0%
CCACATGCATGCATCTTTCC+AGG - chr2.4:3912408-3912427 None:intergenic 50.0%
CCTGGAAAGATGCATGCATG+TGG + chr2.4:3912405-3912424 MS.gene85177:CDS 50.0%
CCTGGATACGTCTTCATCGT+CGG + chr2.4:3914010-3914029 MS.gene85177:CDS 50.0%
GAATCCGGCTTACGTGCAAT+TGG + chr2.4:3912706-3912725 MS.gene85177:intron 50.0%
GATGCTCTTCTCATGCAGGT+AGG + chr2.4:3912238-3912257 MS.gene85177:intron 50.0%
TCCCACTCCACCAATTCCTA+AGG - chr2.4:3912369-3912388 None:intergenic 50.0%
TGCGTTGCATCTATGCACAG+CGG - chr2.4:3913819-3913838 None:intergenic 50.0%
TGTCGCTCTTAGAGCTGACA+TGG + chr2.4:3912216-3912235 MS.gene85177:CDS 50.0%
! AGTGTTGCTGGGAGTTTGCT+CGG + chr2.4:3913917-3913936 MS.gene85177:CDS 50.0%
!! CATGTTGAGGATTGGCTGCA+TGG - chr2.4:3913124-3913143 None:intergenic 50.0%
!! GAAGGTGCCACCAATTGTGA+CGG - chr2.4:3913559-3913578 None:intergenic 50.0%
!! GCTCTAAGAGCGACAAAAGG+AGG - chr2.4:3912211-3912230 None:intergenic 50.0%
!! TGGTGCTGCTAAGATCCTCA+AGG + chr2.4:3912455-3912474 MS.gene85177:CDS 50.0%
ACGTGAAGTGCAGCGCCATA+AGG - chr2.4:3914109-3914128 None:intergenic 55.0%
AGCAGAAGAAGGAGGTGGTG+GGG + chr2.4:3912941-3912960 MS.gene85177:CDS 55.0%
AGGTCAGGTCCTATGCTTGC+AGG + chr2.4:3913059-3913078 MS.gene85177:CDS 55.0%
CAACCAGCAGAAGAAGGAGG+TGG + chr2.4:3912936-3912955 MS.gene85177:CDS 55.0%
CAGCAGAAGAAGGAGGTGGT+GGG + chr2.4:3912940-3912959 MS.gene85177:CDS 55.0%
CCAGACTCCGTCACAATTGG+TGG + chr2.4:3913549-3913568 MS.gene85177:CDS 55.0%
CCGACGATGAAGACGTATCC+AGG - chr2.4:3914013-3914032 None:intergenic 55.0%
GCTGTAATAAGCGGCAAGGG+AGG + chr2.4:3913098-3913117 MS.gene85177:CDS 55.0%
GGTGAAGTGGCATCTAGGTC+AGG + chr2.4:3913044-3913063 MS.gene85177:CDS 55.0%
TCAGGTCCTATGCTTGCAGG+AGG + chr2.4:3913062-3913081 MS.gene85177:CDS 55.0%
! CCACCAATTGTGACGGAGTC+TGG - chr2.4:3913552-3913571 None:intergenic 55.0%
!! CAGAAGGTACCAGCGGTAGA+TGG + chr2.4:3914163-3914182 MS.gene85177:CDS 55.0%
AGGCCACCTCCTGCAAGCAT+AGG - chr2.4:3913071-3913090 None:intergenic 60.0%
CCACCACCTCCTTCTTCTGC+TGG - chr2.4:3912942-3912961 None:intergenic 60.0%
CCAGCAGAAGAAGGAGGTGG+TGG + chr2.4:3912939-3912958 MS.gene85177:CDS 60.0%
GGTCCTATGCTTGCAGGAGG+TGG + chr2.4:3913065-3913084 MS.gene85177:CDS 60.0%
GGCTGATCCCCTTGACACCC+AGG + chr2.4:3913208-3913227 MS.gene85177:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 3912049 3914207 3912049 ID=MS.gene85177
chr2.4 mRNA 3912049 3914207 3912049 ID=MS.gene85177.t1;Parent=MS.gene85177
chr2.4 exon 3912049 3912255 3912049 ID=MS.gene85177.t1.exon1;Parent=MS.gene85177.t1
chr2.4 CDS 3912049 3912255 3912049 ID=cds.MS.gene85177.t1;Parent=MS.gene85177.t1
chr2.4 exon 3912372 3912497 3912372 ID=MS.gene85177.t1.exon2;Parent=MS.gene85177.t1
chr2.4 CDS 3912372 3912497 3912372 ID=cds.MS.gene85177.t1;Parent=MS.gene85177.t1
chr2.4 exon 3912915 3913229 3912915 ID=MS.gene85177.t1.exon3;Parent=MS.gene85177.t1
chr2.4 CDS 3912915 3913229 3912915 ID=cds.MS.gene85177.t1;Parent=MS.gene85177.t1
chr2.4 exon 3913501 3913629 3913501 ID=MS.gene85177.t1.exon4;Parent=MS.gene85177.t1
chr2.4 CDS 3913501 3913629 3913501 ID=cds.MS.gene85177.t1;Parent=MS.gene85177.t1
chr2.4 exon 3913800 3914207 3913800 ID=MS.gene85177.t1.exon5;Parent=MS.gene85177.t1
chr2.4 CDS 3913800 3914207 3913800 ID=cds.MS.gene85177.t1;Parent=MS.gene85177.t1
Gene Sequence

>MS.gene85177

ATGGTTGATATAAGGAGGAAGATTCATGAGAATCCTGAACTTGGTTATGAGGAATTTGAAACCAGTAAGCTTATAAGAACAAAACTGGATGAATTAGGTGTCCCATATAAACATCCAGTTGCAGTGACAGGTGTTATTGGTTACATAGGAACTGGTCTTCCTCCTTTTGTCGCTCTTAGAGCTGACATGGATGCTCTTCTCATGCAGGTAGGTCTACATTTTTTTCTGAGTGTTTTGGTTTTCAACCATATATTATCATGGATTTTATATTAGGATACTGCTTCATCTATATTCCACTGATGATTTTTTTTGGTTAACCTTAGGAATTGGTGGAGTGGGAGCATAAGAGTAAAGTGCCTGGAAAGATGCATGCATGTGGTCATGATGTTCATGTTGCTATGCTTCTTGGTGCTGCTAAGATCCTCAAGGAACATGAAAAACAGTTACAGGTATGGTTTTTAAATCGTGCGTTAAAACTTTAAAGTGTATTGTTTTTGCTTTATTTTATTATTTTCAATTACTTGTGTGGAGAAAACAAAAAAAAAAAAAATAAAGTTGAAAAGGTGTGGTGTTGTCTTCAAACCAAGCATGCCATTGAATTGCAGAAACTACAAACATTGTGATCCTTGTGAATTGTGATTGTAAAAAATTGCGAATGAATCCGGCTTACGTGCAATTGGGATGCTATAGTTGCGGAAATTTTTTAAACCTTAACACTGCATAGTGCATATTATACTTTTCTTTTCATCCTTAAAATTAAAGCTTAAAATTGCAGAAACTACTATACCAAGATGACTCCTGCGCATTATACTTTTCTTTCCATCCTTTTATGGATATGTGATATCATTCCTTATTTTTTGGTAAAGGGAACGGTTGTTCTTGTTTTTCAACCAGCAGAAGAAGGAGGTGGTGGGGCCAAGAAAATTTTAGATGCTGGAGCCTTAGAAAATGTCTCTGCCATATTTGGAATGCATGTTGGACCTAACATACCTTTAGGTGAAGTGGCATCTAGGTCAGGTCCTATGCTTGCAGGAGGTGGCCTCTTTAAAGCTGTAATAAGCGGCAAGGGAGGCCATGCAGCCAATCCTCAACATGCTATTGACCCCATTTTGGCGGCTTCGAATGTGATTGTTAGCTTACAACATATCGTTTCTCGCGAGGCTGATCCCCTTGACACCCAGGTAAAAACTTGCAACTCATCATTGTTTGCAACATTAAAAGCTTTGTATTCATTTGGAACTTCTACGTTGATCTGTTCTAACTTATCAATGATAGAAGATAACCACGAATCATACAATACTCATGTTGGAGACACACCATCTTGCGAAATATTTTGGCTGTTTGAGGAAAAAAATGTTGGAAATCATAGTGTAACATTATTTATTTACAATGATTTTTGAATACGATGAAAATCTGAGGTCTGAATGTCTTCCTAAAATAATATCCTCAAAGGTTGTGACTGTAGGAAAGATTCAAGGAGGTGGTGCATTCAATGTTATTCCAGACTCCGTCACAATTGGTGGCACCTTCCGAGCATTTTTGAGAGAAAGCTTCACGCAACTAAGACACCGTATTGAGCAGGTCTGTTACCAAGAAGAACATATTCACAATGATCTCTATATTTTCCACATCATGAATTGTGTCTTATTCTTATTTGTTGATATTCGAATTGTTAGGATACAAATTATATTTATCCTGTTTTAGTTATCACTGAGATCCTGTTACACGCGAAGTCTCTTTCGGTCTTGTAGGTTATCATTGGACAAGCCGCTGTGCATAGATGCAACGCAACAGTGAGCTTCCTTGATGACAAGATACCGTCCTGCCCTCCAACTATAAACAATGATGGCTTGCACGACTATTTTCAAAGTGTTGCTGGGAGTTTGCTCGGCGTTGATAAAGTTAAAGGCCATCATCTATTGATGGGATCAGAAGACTTTGCATTCTATCAAGAGGCTATGCCTGGATACGTCTTCATCGTCGGAATGGAAGATGTCACTGTTGAACGTCTTAGGTCATGGCACTCACCCTATTTCAAAGTCAACGAAGATGTACTTCCTTATGGCGCTGCACTTCACGTCTCATTAGCGACTAGGTATCTTGCCAAGTTAAATCAGAAGGTACCAGCGGTAGATGGAAAATATCATGACGAATTATAG
Protein sequence

>MS.gene85177.t1

MVDIRRKIHENPELGYEEFETSKLIRTKLDELGVPYKHPVAVTGVIGYIGTGLPPFVALRADMDALLMQELVEWEHKSKVPGKMHACGHDVHVAMLLGAAKILKEHEKQLQGTVVLVFQPAEEGGGGAKKILDAGALENVSAIFGMHVGPNIPLGEVASRSGPMLAGGGLFKAVISGKGGHAANPQHAIDPILAASNVIVSLQHIVSREADPLDTQVVTVGKIQGGGAFNVIPDSVTIGGTFRAFLRESFTQLRHRIEQVIIGQAAVHRCNATVSFLDDKIPSCPPTINNDGLHDYFQSVAGSLLGVDKVKGHHLLMGSEDFAFYQEAMPGYVFIVGMEDVTVERLRSWHSPYFKVNEDVLPYGAALHVSLATRYLAKLNQKVPAVDGKYHDEL