AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85280.t1 MTR_3g054090 98.339 301 5 0 1 301 1 301 0.0 603
MS.gene85280.t1 MTR_6g072020 26.433 314 194 9 20 301 18 326 1.43e-17 82.0
MS.gene85280.t1 MTR_7g104550 25.397 252 155 10 46 286 7 236 1.63e-12 67.4
MS.gene85280.t1 MTR_5g016370 24.590 305 155 13 23 284 318 590 2.87e-12 67.4
MS.gene85280.t1 MTR_4g095058 27.660 235 134 10 54 284 371 573 5.89e-12 66.2
MS.gene85280.t1 MTR_4g095058 27.660 235 134 10 54 284 369 571 6.21e-12 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85280.t1 AT2G30050 84.768 302 45 1 1 301 1 302 0.0 531
MS.gene85280.t1 AT3G01340 82.119 302 53 1 1 301 1 302 0.0 521
MS.gene85280.t1 AT3G01340 82.119 302 53 1 1 301 1 302 0.0 521
MS.gene85280.t1 AT4G02730 27.165 254 149 11 46 298 34 252 1.03e-12 68.2
MS.gene85280.t1 AT1G64350 25.180 278 167 11 55 301 56 323 2.71e-12 66.6
MS.gene85280.t1 AT2G26060 27.490 251 148 12 47 285 12 240 2.58e-11 63.9
MS.gene85280.t1 AT2G26060 27.016 248 149 8 4 240 14 240 8.55e-11 62.4

Find 108 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGCTTGGGCACCGAATT+TGG 0.171644 2.4:+5133786 MS.gene85280:CDS
GAGCTTGGATCCATTCATTT+TGG 0.222798 2.4:-5133408 None:intergenic
TTGGTGTGACTTCTGTTTCT+TGG 0.229774 2.4:+5133593 MS.gene85280:CDS
CTGGTCACCAAGGGCCTGTT+TGG 0.241942 2.4:+5133299 MS.gene85280:CDS
ATGACGGACGCGTCATTCTT+TGG 0.264898 2.4:+5133374 MS.gene85280:CDS
CCGGGTGCTCTTGTTGGTTC+AGG 0.273284 2.4:+5133631 MS.gene85280:CDS
GAAACAACAATGTCACATTA+TGG 0.309368 2.4:+5133977 MS.gene85280:CDS
GATCACACAATTAAGATAAT+TGG 0.313304 2.4:+5133238 MS.gene85280:CDS
GCACTAGCAATAGTAGATTT+AGG 0.318347 2.4:-5133814 None:intergenic
GTCTTTGACAGGAAATATAT+TGG 0.319441 2.4:+5133942 MS.gene85280:CDS
CACTTCAGATGCATAATGAT+TGG 0.332563 2.4:+5133755 MS.gene85280:CDS
TGTTTCTTGGGCTCCTTCAA+TGG 0.345050 2.4:+5133606 MS.gene85280:CDS
GTCTGTGAATTCAGTTGCTT+GGG 0.353330 2.4:+5133453 MS.gene85280:CDS
GTTGCTTGGGCACCGAATTT+GGG 0.355435 2.4:+5133787 MS.gene85280:CDS
TGGTGTGACTTCTGTTTCTT+GGG 0.358030 2.4:+5133594 MS.gene85280:CDS
GAAACAGAAGTCACACCAAC+TGG 0.367413 2.4:-5133589 None:intergenic
AAGAATGACGCGTCCGTCAT+AGG 0.368314 2.4:-5133371 None:intergenic
GACCATGATACCCTCCAAAC+AGG 0.377638 2.4:-5133922 None:intergenic
GCTTGGGCGCCTCACGAGTT+GGG 0.386990 2.4:+5133469 MS.gene85280:CDS
ATGGCGCCGGGTGCTCTTGT+TGG 0.389540 2.4:+5133625 MS.gene85280:CDS
TTGGCAAAGAAGGGGATCAT+TGG 0.390865 2.4:+5133869 MS.gene85280:CDS
TGCTTGGGCGCCTCACGAGT+TGG 0.397039 2.4:+5133468 MS.gene85280:CDS
TTGGGCTCCTTCAATGGCGC+CGG 0.406041 2.4:+5133612 MS.gene85280:CDS
TTGGCTTGTGCTTCGTCTGA+TGG 0.406467 2.4:+5133499 MS.gene85280:CDS
TGGTCACCAAGGGCCTGTTT+GGG 0.409839 2.4:+5133300 MS.gene85280:CDS
GCGTGGGCTCACCCTAAGTT+TGG 0.412765 2.4:+5133328 MS.gene85280:CDS
GCCTGTTTGGGAGGTTGCGT+GGG 0.414310 2.4:+5133312 MS.gene85280:CDS
CGTCTGTGAATTCAGTTGCT+TGG 0.416920 2.4:+5133452 MS.gene85280:CDS
TCACGAGTTGGGTCTCTGCT+TGG 0.431902 2.4:+5133480 MS.gene85280:CDS
ATGATTTCAAGACCCCTGTT+TGG 0.434543 2.4:+5133908 MS.gene85280:CDS
TGGGCTCCTTCAATGGCGCC+GGG 0.435343 2.4:+5133613 MS.gene85280:CDS
GGCCTGTTTGGGAGGTTGCG+TGG 0.439174 2.4:+5133311 MS.gene85280:CDS
TTATGGAAAGAAGCTGTAGA+TGG 0.441647 2.4:+5133994 MS.gene85280:CDS
ATATGGACTGTTGGCAAAGA+AGG 0.444237 2.4:+5133859 MS.gene85280:CDS
GATTTAGGAAGTCCCAAATT+CGG 0.447910 2.4:-5133799 None:intergenic
GCCCACGCAACCTCCCAAAC+AGG 0.450330 2.4:-5133313 None:intergenic
AGAGCACCCGGCGCCATTGA+AGG 0.456543 2.4:-5133619 None:intergenic
GTGCAGAAGCTTTGCTCTGG+TGG 0.463692 2.4:+5133667 MS.gene85280:CDS
TGTAGATGGGGAATGGCAAC+AGG 0.465699 2.4:+5134008 MS.gene85280:CDS
TTCGTCTGATGGGAATATTT+CGG 0.470361 2.4:+5133510 MS.gene85280:CDS
ATGGATTATGTCTTGATGAC+CGG 0.472610 2.4:-5133167 None:intergenic
TCATTATGCATCTGAAGTGC+AGG 0.475699 2.4:-5133751 None:intergenic
GCGAGAAGGGAACCAAACTT+AGG 0.477573 2.4:-5133340 None:intergenic
CCCCTGTTTGGAGGGTATCA+TGG 0.478267 2.4:+5133920 MS.gene85280:CDS
TGGCTTGTGCTTCGTCTGAT+GGG 0.479615 2.4:+5133500 MS.gene85280:CDS
GATGTTGCAATGGATTACTA+TGG 0.484612 2.4:+5133190 MS.gene85280:CDS
ACTTCAGATGCATAATGATT+GGG 0.487890 2.4:+5133756 MS.gene85280:CDS
TGGCAAAGAAGGGGATCATT+GGG 0.491121 2.4:+5133870 MS.gene85280:CDS
CTGCAAAGGCAGATGGTGGA+TGG 0.503412 2.4:+5133539 MS.gene85280:CDS
CCTGTGCAGAAGCTTTGCTC+TGG 0.505097 2.4:+5133664 MS.gene85280:CDS
CAGCATCTAGCAACTTTGAC+TGG 0.508215 2.4:+5133280 MS.gene85280:CDS
ATTGGGTTCGAGATGTTGCT+TGG 0.510278 2.4:+5133773 MS.gene85280:CDS
CGCGTCATTCTTTGGAAGGA+GGG 0.511398 2.4:+5133382 MS.gene85280:CDS
AATATATTGGCTGTGGCTGA+TGG 0.512420 2.4:+5133955 MS.gene85280:CDS
TGGTTGTGATAATACTGTTA+AGG 0.515208 2.4:+5133687 MS.gene85280:CDS
TTGGGTTCGAGATGTTGCTT+GGG 0.517736 2.4:+5133774 MS.gene85280:CDS
CCTTCTCAGAAGGTTGAAAC+CGG 0.519832 2.4:+5133148 MS.gene85280:CDS
TATGGAAAGAAGCTGTAGAT+GGG 0.520145 2.4:+5133995 MS.gene85280:CDS
CAACCTCCCAAACAGGCCCT+TGG 0.520290 2.4:-5133306 None:intergenic
AGTAATCCATTGCAACATCA+TGG 0.520730 2.4:-5133186 None:intergenic
TCACCAAGGGCCTGTTTGGG+AGG 0.524084 2.4:+5133303 MS.gene85280:CDS
AGGGTATCATGGTCTTTGAC+AGG 0.524931 2.4:+5133931 MS.gene85280:CDS
AAGACGGTAAAGTGATCATA+TGG 0.528476 2.4:+5133842 MS.gene85280:CDS
TATGGACTGTTGGCAAAGAA+GGG 0.531973 2.4:+5133860 MS.gene85280:CDS
AGGAGGGTAACCAAAATGAA+TGG 0.536407 2.4:+5133398 MS.gene85280:CDS
TAGCAATGGACAGTGGAAGA+TGG 0.537404 2.4:+5133720 MS.gene85280:CDS
ATTTCAAGACCCCTGTTTGG+AGG 0.539676 2.4:+5133911 MS.gene85280:CDS
CGAGAAGGGAACCAAACTTA+GGG 0.543581 2.4:-5133339 None:intergenic
GAGGCTAGCAACAGCATCAT+CGG 0.547815 2.4:+5133216 MS.gene85280:CDS
CAGAAACATGCCTTCTCAGA+AGG 0.548126 2.4:+5133138 None:intergenic
GTATTTACTGCAAAGGCAGA+TGG 0.549821 2.4:+5133532 MS.gene85280:CDS
GACAGGAAATATATTGGCTG+TGG 0.552846 2.4:+5133948 MS.gene85280:CDS
GCTCATCAAAGACATGAGCT+TGG 0.554760 2.4:-5133423 None:intergenic
CATAGGAACATGAAGCGAGA+AGG 0.556637 2.4:-5133354 None:intergenic
AGGGGATCATTGGGAAGGCA+AGG 0.557835 2.4:+5133879 MS.gene85280:CDS
CAATGGATTACTATGGAAAG+AGG 0.559724 2.4:+5133197 MS.gene85280:CDS
CATAATCCATGATGTTGCAA+TGG 0.560125 2.4:+5133180 MS.gene85280:CDS
CTCGCTTCATGTTCCTATGA+CGG 0.561648 2.4:+5133358 MS.gene85280:CDS
CCGGTTTCAACCTTCTGAGA+AGG 0.569075 2.4:-5133148 None:intergenic
TTTCAAGACCCCTGTTTGGA+GGG 0.570292 2.4:+5133912 MS.gene85280:CDS
AAAGTGATCATATGGACTGT+TGG 0.571303 2.4:+5133850 MS.gene85280:CDS
CGGACGCGTCATTCTTTGGA+AGG 0.574371 2.4:+5133378 MS.gene85280:CDS
TGCAAAGGCAGATGGTGGAT+GGG 0.576134 2.4:+5133540 MS.gene85280:CDS
TATTTCGGTATTTACTGCAA+AGG 0.580846 2.4:+5133525 MS.gene85280:CDS
ACGCGTCATTCTTTGGAAGG+AGG 0.581061 2.4:+5133381 MS.gene85280:CDS
CCAGAGCAAAGCTTCTGCAC+AGG 0.586753 2.4:-5133664 None:intergenic
ACCATGATACCCTCCAAACA+GGG 0.587238 2.4:-5133921 None:intergenic
ATGGTGGATGGGACACCTCA+AGG 0.588008 2.4:+5133551 MS.gene85280:CDS
ATTGCTAGTGCGTCGCAAGA+CGG 0.588884 2.4:+5133826 MS.gene85280:CDS
AAGAAGCTGTAGATGGGGAA+TGG 0.590067 2.4:+5134001 MS.gene85280:CDS
ATAGGAACATGAAGCGAGAA+GGG 0.592233 2.4:-5133353 None:intergenic
CCTGAACCAACAAGAGCACC+CGG 0.595135 2.4:-5133631 None:intergenic
TTTACTGCAAAGGCAGATGG+TGG 0.615017 2.4:+5133535 MS.gene85280:CDS
CATTATGCATCTGAAGTGCA+GGG 0.623511 2.4:-5133750 None:intergenic
AAGCAGAGACCCAACTCGTG+AGG 0.625403 2.4:-5133478 None:intergenic
ATTGATCAAGCTCACCCAGT+TGG 0.626929 2.4:+5133574 MS.gene85280:CDS
CAACTTTGACTGGTCACCAA+GGG 0.628577 2.4:+5133290 MS.gene85280:CDS
ATGGAAAGAAGCTGTAGATG+GGG 0.635633 2.4:+5133996 MS.gene85280:CDS
ATGGACTGTTGGCAAAGAAG+GGG 0.637383 2.4:+5133861 MS.gene85280:CDS
AAACAGAAGTCACACCAACT+GGG 0.643078 2.4:-5133588 None:intergenic
GCAACTTTGACTGGTCACCA+AGG 0.656777 2.4:+5133289 MS.gene85280:CDS
AAGGTGTGGAAGCTTAGCAA+TGG 0.658169 2.4:+5133706 MS.gene85280:CDS
GTGAGCTTGATCAATCCTTG+AGG 0.660407 2.4:-5133566 None:intergenic
AAAGAAGGGGATCATTGGGA+AGG 0.667803 2.4:+5133874 MS.gene85280:CDS
GGAAGCTTAGCAATGGACAG+TGG 0.684704 2.4:+5133713 MS.gene85280:CDS
ATGGCAACAGGTGACAACAG+TGG 0.686621 2.4:+5134020 MS.gene85280:CDS
GTGATAATACTGTTAAGGTG+TGG 0.686828 2.4:+5133692 MS.gene85280:CDS
CCATGATACCCTCCAAACAG+GGG 0.724647 2.4:-5133920 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GATCACACAATTAAGATAAT+TGG + chr2.4:5133238-5133257 MS.gene85280:CDS 25.0%
ACTTCAGATGCATAATGATT+GGG + chr2.4:5133756-5133775 MS.gene85280:CDS 30.0%
GAAACAACAATGTCACATTA+TGG + chr2.4:5133977-5133996 MS.gene85280:CDS 30.0%
GTCTTTGACAGGAAATATAT+TGG + chr2.4:5133942-5133961 MS.gene85280:CDS 30.0%
TATTTCGGTATTTACTGCAA+AGG + chr2.4:5133525-5133544 MS.gene85280:CDS 30.0%
TGGTTGTGATAATACTGTTA+AGG + chr2.4:5133687-5133706 MS.gene85280:CDS 30.0%
AAGACGGTAAAGTGATCATA+TGG + chr2.4:5133842-5133861 MS.gene85280:CDS 35.0%
AGTAATCCATTGCAACATCA+TGG - chr2.4:5133189-5133208 None:intergenic 35.0%
CACTTCAGATGCATAATGAT+TGG + chr2.4:5133755-5133774 MS.gene85280:CDS 35.0%
CATAATCCATGATGTTGCAA+TGG + chr2.4:5133180-5133199 MS.gene85280:CDS 35.0%
GATTTAGGAAGTCCCAAATT+CGG - chr2.4:5133802-5133821 None:intergenic 35.0%
GCACTAGCAATAGTAGATTT+AGG - chr2.4:5133817-5133836 None:intergenic 35.0%
GTGATAATACTGTTAAGGTG+TGG + chr2.4:5133692-5133711 MS.gene85280:CDS 35.0%
TATGGAAAGAAGCTGTAGAT+GGG + chr2.4:5133995-5134014 MS.gene85280:CDS 35.0%
TTATGGAAAGAAGCTGTAGA+TGG + chr2.4:5133994-5134013 MS.gene85280:CDS 35.0%
TTCGTCTGATGGGAATATTT+CGG + chr2.4:5133510-5133529 MS.gene85280:CDS 35.0%
! AAAGTGATCATATGGACTGT+TGG + chr2.4:5133850-5133869 MS.gene85280:CDS 35.0%
! ATGGATTATGTCTTGATGAC+CGG - chr2.4:5133170-5133189 None:intergenic 35.0%
!! CAATGGATTACTATGGAAAG+AGG + chr2.4:5133197-5133216 MS.gene85280:CDS 35.0%
!! GATGTTGCAATGGATTACTA+TGG + chr2.4:5133190-5133209 MS.gene85280:CDS 35.0%
AAACAGAAGTCACACCAACT+GGG - chr2.4:5133591-5133610 None:intergenic 40.0%
AGGAGGGTAACCAAAATGAA+TGG + chr2.4:5133398-5133417 MS.gene85280:CDS 40.0%
ATAGGAACATGAAGCGAGAA+GGG - chr2.4:5133356-5133375 None:intergenic 40.0%
ATGATTTCAAGACCCCTGTT+TGG + chr2.4:5133908-5133927 MS.gene85280:CDS 40.0%
ATGGAAAGAAGCTGTAGATG+GGG + chr2.4:5133996-5134015 MS.gene85280:CDS 40.0%
GTATTTACTGCAAAGGCAGA+TGG + chr2.4:5133532-5133551 MS.gene85280:CDS 40.0%
GTCTGTGAATTCAGTTGCTT+GGG + chr2.4:5133453-5133472 MS.gene85280:CDS 40.0%
! AATATATTGGCTGTGGCTGA+TGG + chr2.4:5133955-5133974 MS.gene85280:CDS 40.0%
! ATATGGACTGTTGGCAAAGA+AGG + chr2.4:5133859-5133878 MS.gene85280:CDS 40.0%
! CATTATGCATCTGAAGTGCA+GGG - chr2.4:5133753-5133772 None:intergenic 40.0%
! GACAGGAAATATATTGGCTG+TGG + chr2.4:5133948-5133967 MS.gene85280:CDS 40.0%
! GAGCTTGGATCCATTCATTT+TGG - chr2.4:5133411-5133430 None:intergenic 40.0%
! TATGGACTGTTGGCAAAGAA+GGG + chr2.4:5133860-5133879 MS.gene85280:CDS 40.0%
! TCATTATGCATCTGAAGTGC+AGG - chr2.4:5133754-5133773 None:intergenic 40.0%
! TGGTGTGACTTCTGTTTCTT+GGG + chr2.4:5133594-5133613 MS.gene85280:CDS 40.0%
! TTGGTGTGACTTCTGTTTCT+TGG + chr2.4:5133593-5133612 MS.gene85280:CDS 40.0%
AAAGAAGGGGATCATTGGGA+AGG + chr2.4:5133874-5133893 MS.gene85280:CDS 45.0%
AAGAAGCTGTAGATGGGGAA+TGG + chr2.4:5134001-5134020 MS.gene85280:CDS 45.0%
AAGGTGTGGAAGCTTAGCAA+TGG + chr2.4:5133706-5133725 MS.gene85280:CDS 45.0%
ACCATGATACCCTCCAAACA+GGG - chr2.4:5133924-5133943 None:intergenic 45.0%
ATTGATCAAGCTCACCCAGT+TGG + chr2.4:5133574-5133593 MS.gene85280:CDS 45.0%
ATTGGGTTCGAGATGTTGCT+TGG + chr2.4:5133773-5133792 MS.gene85280:CDS 45.0%
ATTTCAAGACCCCTGTTTGG+AGG + chr2.4:5133911-5133930 MS.gene85280:CDS 45.0%
CAGCATCTAGCAACTTTGAC+TGG + chr2.4:5133280-5133299 MS.gene85280:CDS 45.0%
CATAGGAACATGAAGCGAGA+AGG - chr2.4:5133357-5133376 None:intergenic 45.0%
CCTTCTCAGAAGGTTGAAAC+CGG + chr2.4:5133148-5133167 MS.gene85280:CDS 45.0%
CGAGAAGGGAACCAAACTTA+GGG - chr2.4:5133342-5133361 None:intergenic 45.0%
CGTCTGTGAATTCAGTTGCT+TGG + chr2.4:5133452-5133471 MS.gene85280:CDS 45.0%
CTCGCTTCATGTTCCTATGA+CGG + chr2.4:5133358-5133377 MS.gene85280:CDS 45.0%
GAAACAGAAGTCACACCAAC+TGG - chr2.4:5133592-5133611 None:intergenic 45.0%
GCTCATCAAAGACATGAGCT+TGG - chr2.4:5133426-5133445 None:intergenic 45.0%
GTGAGCTTGATCAATCCTTG+AGG - chr2.4:5133569-5133588 None:intergenic 45.0%
TAGCAATGGACAGTGGAAGA+TGG + chr2.4:5133720-5133739 MS.gene85280:CDS 45.0%
TGGCAAAGAAGGGGATCATT+GGG + chr2.4:5133870-5133889 MS.gene85280:CDS 45.0%
TGTTTCTTGGGCTCCTTCAA+TGG + chr2.4:5133606-5133625 MS.gene85280:CDS 45.0%
TTGGCAAAGAAGGGGATCAT+TGG + chr2.4:5133869-5133888 MS.gene85280:CDS 45.0%
TTTACTGCAAAGGCAGATGG+TGG + chr2.4:5133535-5133554 MS.gene85280:CDS 45.0%
TTTCAAGACCCCTGTTTGGA+GGG + chr2.4:5133912-5133931 MS.gene85280:CDS 45.0%
! AGGGTATCATGGTCTTTGAC+AGG + chr2.4:5133931-5133950 MS.gene85280:CDS 45.0%
! ATGGACTGTTGGCAAAGAAG+GGG + chr2.4:5133861-5133880 MS.gene85280:CDS 45.0%
! CAACTTTGACTGGTCACCAA+GGG + chr2.4:5133290-5133309 MS.gene85280:CDS 45.0%
! TTGGGTTCGAGATGTTGCTT+GGG + chr2.4:5133774-5133793 MS.gene85280:CDS 45.0%
AAGAATGACGCGTCCGTCAT+AGG - chr2.4:5133374-5133393 None:intergenic 50.0%
ATGGCAACAGGTGACAACAG+TGG + chr2.4:5134020-5134039 MS.gene85280:CDS 50.0%
ATTGCTAGTGCGTCGCAAGA+CGG + chr2.4:5133826-5133845 MS.gene85280:CDS 50.0%
CCATGATACCCTCCAAACAG+GGG - chr2.4:5133923-5133942 None:intergenic 50.0%
GACCATGATACCCTCCAAAC+AGG - chr2.4:5133925-5133944 None:intergenic 50.0%
GAGGCTAGCAACAGCATCAT+CGG + chr2.4:5133216-5133235 MS.gene85280:CDS 50.0%
GCGAGAAGGGAACCAAACTT+AGG - chr2.4:5133343-5133362 None:intergenic 50.0%
GGAAGCTTAGCAATGGACAG+TGG + chr2.4:5133713-5133732 MS.gene85280:CDS 50.0%
TGTAGATGGGGAATGGCAAC+AGG + chr2.4:5134008-5134027 MS.gene85280:CDS 50.0%
! ACGCGTCATTCTTTGGAAGG+AGG + chr2.4:5133381-5133400 MS.gene85280:CDS 50.0%
! CCGGTTTCAACCTTCTGAGA+AGG - chr2.4:5133151-5133170 None:intergenic 50.0%
! CGCGTCATTCTTTGGAAGGA+GGG + chr2.4:5133382-5133401 MS.gene85280:CDS 50.0%
! GCAACTTTGACTGGTCACCA+AGG + chr2.4:5133289-5133308 MS.gene85280:CDS 50.0%
! TGGCTTGTGCTTCGTCTGAT+GGG + chr2.4:5133500-5133519 MS.gene85280:CDS 50.0%
!! ATGACGGACGCGTCATTCTT+TGG + chr2.4:5133374-5133393 MS.gene85280:CDS 50.0%
!! GTTGCTTGGGCACCGAATTT+GGG + chr2.4:5133787-5133806 MS.gene85280:CDS 50.0%
!! TGCAAAGGCAGATGGTGGAT+GGG + chr2.4:5133540-5133559 MS.gene85280:CDS 50.0%
!! TGTTGCTTGGGCACCGAATT+TGG + chr2.4:5133786-5133805 MS.gene85280:CDS 50.0%
!! TTGGCTTGTGCTTCGTCTGA+TGG + chr2.4:5133499-5133518 MS.gene85280:CDS 50.0%
AAGCAGAGACCCAACTCGTG+AGG - chr2.4:5133481-5133500 None:intergenic 55.0%
AGGGGATCATTGGGAAGGCA+AGG + chr2.4:5133879-5133898 MS.gene85280:CDS 55.0%
CCAGAGCAAAGCTTCTGCAC+AGG - chr2.4:5133667-5133686 None:intergenic 55.0%
CCTGTGCAGAAGCTTTGCTC+TGG + chr2.4:5133664-5133683 MS.gene85280:CDS 55.0%
CGGACGCGTCATTCTTTGGA+AGG + chr2.4:5133378-5133397 MS.gene85280:CDS 55.0%
GTGCAGAAGCTTTGCTCTGG+TGG + chr2.4:5133667-5133686 MS.gene85280:CDS 55.0%
TGGTCACCAAGGGCCTGTTT+GGG + chr2.4:5133300-5133319 MS.gene85280:CDS 55.0%
! TCACGAGTTGGGTCTCTGCT+TGG + chr2.4:5133480-5133499 MS.gene85280:CDS 55.0%
!! ATGGTGGATGGGACACCTCA+AGG + chr2.4:5133551-5133570 MS.gene85280:CDS 55.0%
!! CCCCTGTTTGGAGGGTATCA+TGG + chr2.4:5133920-5133939 MS.gene85280:CDS 55.0%
!! CCTGAACCAACAAGAGCACC+CGG - chr2.4:5133634-5133653 None:intergenic 55.0%
!! CTGCAAAGGCAGATGGTGGA+TGG + chr2.4:5133539-5133558 MS.gene85280:CDS 55.0%
CAACCTCCCAAACAGGCCCT+TGG - chr2.4:5133309-5133328 None:intergenic 60.0%
CTGGTCACCAAGGGCCTGTT+TGG + chr2.4:5133299-5133318 MS.gene85280:CDS 60.0%
GCCTGTTTGGGAGGTTGCGT+GGG + chr2.4:5133312-5133331 MS.gene85280:CDS 60.0%
GCGTGGGCTCACCCTAAGTT+TGG + chr2.4:5133328-5133347 MS.gene85280:CDS 60.0%
TCACCAAGGGCCTGTTTGGG+AGG + chr2.4:5133303-5133322 MS.gene85280:CDS 60.0%
TTGGGCTCCTTCAATGGCGC+CGG + chr2.4:5133612-5133631 MS.gene85280:CDS 60.0%
!! CCGGGTGCTCTTGTTGGTTC+AGG + chr2.4:5133631-5133650 MS.gene85280:CDS 60.0%
GCCCACGCAACCTCCCAAAC+AGG - chr2.4:5133316-5133335 None:intergenic 65.0%
GCTTGGGCGCCTCACGAGTT+GGG + chr2.4:5133469-5133488 MS.gene85280:CDS 65.0%
GGCCTGTTTGGGAGGTTGCG+TGG + chr2.4:5133311-5133330 MS.gene85280:CDS 65.0%
TGCTTGGGCGCCTCACGAGT+TGG + chr2.4:5133468-5133487 MS.gene85280:CDS 65.0%
TGGGCTCCTTCAATGGCGCC+GGG + chr2.4:5133613-5133632 MS.gene85280:CDS 65.0%
! AGAGCACCCGGCGCCATTGA+AGG - chr2.4:5133622-5133641 None:intergenic 65.0%
! ATGGCGCCGGGTGCTCTTGT+TGG + chr2.4:5133625-5133644 MS.gene85280:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 5133145 5134050 5133145 ID=MS.gene85280
chr2.4 mRNA 5133145 5134050 5133145 ID=MS.gene85280.t1;Parent=MS.gene85280
chr2.4 exon 5133145 5134050 5133145 ID=MS.gene85280.t1.exon1;Parent=MS.gene85280.t1
chr2.4 CDS 5133145 5134050 5133145 ID=cds.MS.gene85280.t1;Parent=MS.gene85280.t1
Gene Sequence

>MS.gene85280

ATGCCTTCTCAGAAGGTTGAAACCGGTCATCAAGACATAATCCATGATGTTGCAATGGATTACTATGGAAAGAGGCTAGCAACAGCATCATCGGATCACACAATTAAGATAATTGGTGTAAGCAATTCAGCTTCTCAGCATCTAGCAACTTTGACTGGTCACCAAGGGCCTGTTTGGGAGGTTGCGTGGGCTCACCCTAAGTTTGGTTCCCTTCTCGCTTCATGTTCCTATGACGGACGCGTCATTCTTTGGAAGGAGGGTAACCAAAATGAATGGATCCAAGCTCATGTCTTTGATGAGCATAAGTCGTCTGTGAATTCAGTTGCTTGGGCGCCTCACGAGTTGGGTCTCTGCTTGGCTTGTGCTTCGTCTGATGGGAATATTTCGGTATTTACTGCAAAGGCAGATGGTGGATGGGACACCTCAAGGATTGATCAAGCTCACCCAGTTGGTGTGACTTCTGTTTCTTGGGCTCCTTCAATGGCGCCGGGTGCTCTTGTTGGTTCAGGATTACTTGATCCTGTGCAGAAGCTTTGCTCTGGTGGTTGTGATAATACTGTTAAGGTGTGGAAGCTTAGCAATGGACAGTGGAAGATGGATTGCTTCCCTGCACTTCAGATGCATAATGATTGGGTTCGAGATGTTGCTTGGGCACCGAATTTGGGACTTCCTAAATCTACTATTGCTAGTGCGTCGCAAGACGGTAAAGTGATCATATGGACTGTTGGCAAAGAAGGGGATCATTGGGAAGGCAAGGTTTTGAATGATTTCAAGACCCCTGTTTGGAGGGTATCATGGTCTTTGACAGGAAATATATTGGCTGTGGCTGATGGAAACAACAATGTCACATTATGGAAAGAAGCTGTAGATGGGGAATGGCAACAGGTGACAACAGTGGAGCCATAG
Protein sequence

>MS.gene85280.t1

MPSQKVETGHQDIIHDVAMDYYGKRLATASSDHTIKIIGVSNSASQHLATLTGHQGPVWEVAWAHPKFGSLLASCSYDGRVILWKEGNQNEWIQAHVFDEHKSSVNSVAWAPHELGLCLACASSDGNISVFTAKADGGWDTSRIDQAHPVGVTSVSWAPSMAPGALVGSGLLDPVQKLCSGGCDNTVKVWKLSNGQWKMDCFPALQMHNDWVRDVAWAPNLGLPKSTIASASQDGKVIIWTVGKEGDHWEGKVLNDFKTPVWRVSWSLTGNILAVADGNNNVTLWKEAVDGEWQQVTTVEP