AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85293

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85293.t1 MTR_2g045360 95.571 429 18 1 1 428 1 429 0.0 809
MS.gene85293.t1 MTR_3g053330 47.162 229 108 6 185 409 225 444 6.48e-57 194
MS.gene85293.t1 MTR_7g110720 34.722 360 187 11 64 415 92 411 4.32e-52 181
MS.gene85293.t1 MTR_7g110720 40.741 270 131 9 157 415 133 384 3.62e-51 177
MS.gene85293.t1 MTR_4g132430 36.691 278 161 4 68 338 114 383 3.00e-48 172
MS.gene85293.t1 MTR_2g088000 35.381 407 180 15 66 408 167 554 3.83e-48 174
MS.gene85293.t1 MTR_4g132430 37.743 257 145 4 89 338 1 249 2.09e-46 164
MS.gene85293.t1 MTR_2g023930 32.506 403 199 11 68 411 143 531 5.52e-44 161
MS.gene85293.t1 MTR_4g049790 34.848 330 168 7 68 357 131 453 9.47e-43 158
MS.gene85293.t1 MTR_4g049760 54.430 158 65 3 182 338 261 412 2.25e-40 151
MS.gene85293.t1 MTR_4g094412 50.658 152 64 4 195 338 227 375 3.40e-37 142
MS.gene85293.t1 MTR_4g094412 50.658 152 64 4 195 338 218 366 3.94e-37 141
MS.gene85293.t1 MTR_4g107970 63.158 95 31 1 181 271 144 238 5.90e-37 142
MS.gene85293.t1 MTR_4g107970 63.158 95 31 1 181 271 221 315 1.13e-36 142
MS.gene85293.t1 MTR_7g009730 61.386 101 38 1 185 284 192 292 3.03e-36 140
MS.gene85293.t1 MTR_4g047580 37.165 261 137 8 166 408 239 490 4.11e-33 131
MS.gene85293.t1 MTR_4g007060 32.110 218 97 8 192 406 107 276 4.70e-24 101
MS.gene85293.t1 MTR_4g007060 42.857 105 58 2 192 294 141 245 6.76e-24 101
MS.gene85293.t1 MTR_5g074400 49.020 102 50 2 172 273 382 481 1.91e-23 103
MS.gene85293.t1 MTR_5g074400 46.667 60 30 2 212 271 244 301 9.29e-11 64.3
MS.gene85293.t1 MTR_2g105060 39.850 133 70 3 194 321 72 199 6.39e-23 97.4
MS.gene85293.t1 MTR_2g105060 39.850 133 70 3 194 321 147 274 1.45e-22 97.8
MS.gene85293.t1 MTR_7g109800 37.671 146 80 4 195 330 129 273 1.92e-22 97.1
MS.gene85293.t1 MTR_1g013790 48.235 85 42 2 209 291 99 183 3.58e-22 95.5
MS.gene85293.t1 MTR_3g106060 50.602 83 38 1 209 291 136 215 3.70e-22 96.3
MS.gene85293.t1 MTR_1g013760 50.685 73 36 0 196 268 125 197 6.75e-22 95.5
MS.gene85293.t1 MTR_5g094430 45.556 90 48 1 195 284 236 324 2.56e-21 95.9
MS.gene85293.t1 MTR_5g094430 45.556 90 48 1 195 284 214 302 2.73e-21 95.5
MS.gene85293.t1 MTR_3g009470 41.667 120 64 3 184 299 464 581 4.22e-21 96.3
MS.gene85293.t1 MTR_3g009470 39.362 94 54 3 183 275 251 342 1.31e-13 73.2
MS.gene85293.t1 MTR_5g094430 50.633 79 38 1 195 273 348 425 4.76e-21 95.9
MS.gene85293.t1 MTR_7g079010 35.088 171 85 6 116 281 58 207 6.39e-21 91.3
MS.gene85293.t1 MTR_7g100510 41.129 124 65 3 169 287 314 434 6.70e-21 94.7
MS.gene85293.t1 MTR_7g100510 42.308 78 43 2 212 289 190 265 3.90e-12 68.2
MS.gene85293.t1 MTR_3g031220 51.282 78 37 1 194 271 348 424 1.19e-20 94.4
MS.gene85293.t1 MTR_5g044695 65.517 58 19 1 183 239 48 105 1.64e-20 86.7
MS.gene85293.t1 MTR_3g056100 50.633 79 38 1 195 273 255 332 1.68e-20 93.6
MS.gene85293.t1 MTR_3g056100 50.633 79 38 1 195 273 242 319 1.70e-20 93.6
MS.gene85293.t1 MTR_3g056100 50.633 79 38 1 195 273 386 463 1.92e-20 94.0
MS.gene85293.t1 MTR_3g056100 50.633 79 38 1 195 273 399 476 2.03e-20 94.0
MS.gene85293.t1 MTR_4g013260 50.000 82 37 2 193 271 315 395 3.75e-20 92.8
MS.gene85293.t1 MTR_4g013260 36.975 119 69 4 163 279 137 251 9.91e-13 70.1
MS.gene85293.t1 MTR_7g011820 47.674 86 44 1 198 283 397 481 7.53e-20 92.0
MS.gene85293.t1 MTR_7g011820 48.333 60 29 2 212 271 229 286 1.57e-11 66.6
MS.gene85293.t1 MTR_4g082580 45.977 87 46 1 196 282 263 348 7.82e-20 91.3
MS.gene85293.t1 MTR_7g080020 48.148 81 41 1 193 273 475 554 1.06e-19 92.0
MS.gene85293.t1 MTR_7g080000 36.641 131 70 2 193 311 414 543 1.31e-19 91.7
MS.gene85293.t1 MTR_5g042920 45.161 93 47 2 198 287 307 398 2.10e-19 90.1
MS.gene85293.t1 MTR_5g042920 50.000 60 28 2 212 271 152 209 6.87e-12 67.4
MS.gene85293.t1 MTR_3g095040 51.351 74 35 1 198 271 105 177 2.37e-19 86.3
MS.gene85293.t1 MTR_4g122530 47.674 86 43 2 195 280 192 275 2.39e-19 90.1
MS.gene85293.t1 MTR_6g038890 65.517 58 19 1 183 239 159 216 2.69e-19 86.7
MS.gene85293.t1 MTR_2g024350 49.296 71 36 0 212 282 9 79 4.66e-19 85.9
MS.gene85293.t1 MTR_2g033820 45.122 82 44 1 195 276 187 267 6.47e-19 88.2
MS.gene85293.t1 MTR_5g097490 40.708 113 61 3 182 290 223 333 8.24e-19 88.2
MS.gene85293.t1 MTR_7g028415 45.263 95 47 2 178 268 56 149 9.18e-19 83.6
MS.gene85293.t1 MTR_7g028710 45.263 95 47 2 178 268 56 149 9.18e-19 83.6
MS.gene85293.t1 MTR_1g086790 44.444 81 44 1 195 275 142 221 1.71e-18 86.3
MS.gene85293.t1 MTR_5g091390 44.872 78 42 1 198 275 144 220 1.96e-18 85.9
MS.gene85293.t1 MTR_7g071120 39.844 128 69 5 198 319 311 436 2.09e-18 87.8
MS.gene85293.t1 MTR_4g094588 35.252 139 70 4 198 323 165 296 2.66e-18 85.9
MS.gene85293.t1 MTR_8g092010 47.297 74 38 1 198 271 95 167 3.08e-18 82.8
MS.gene85293.t1 MTR_2g024380 50.704 71 35 0 212 282 252 322 4.92e-18 86.3
MS.gene85293.t1 MTR_2g016770 55.738 61 27 0 212 272 36 96 6.12e-18 82.4
MS.gene85293.t1 MTR_1g015140 49.315 73 36 1 196 268 87 158 6.31e-18 81.3
MS.gene85293.t1 MTR_2g016770 55.738 61 27 0 212 272 28 88 7.05e-18 82.4
MS.gene85293.t1 MTR_4g094908 54.839 62 28 0 212 273 267 328 1.00e-17 84.3
MS.gene85293.t1 MTR_3g104750 39.560 91 54 1 178 268 71 160 1.91e-17 79.7
MS.gene85293.t1 MTR_8g096730 36.893 103 61 2 181 280 37 138 3.60e-17 78.6
MS.gene85293.t1 MTR_8g087000 56.667 60 26 0 212 271 78 137 4.53e-17 80.9
MS.gene85293.t1 MTR_8g087000 56.667 60 26 0 212 271 77 136 4.90e-17 80.5
MS.gene85293.t1 MTR_5g016610 41.837 98 48 1 182 270 161 258 5.87e-17 80.9
MS.gene85293.t1 MTR_4g130900 44.156 77 42 1 195 271 343 418 8.99e-17 82.8
MS.gene85293.t1 MTR_5g043880 42.683 82 46 1 188 269 101 181 9.60e-17 80.5
MS.gene85293.t1 MTR_1g077780 44.156 77 42 1 195 271 46 121 1.06e-16 77.0
MS.gene85293.t1 MTR_7g109600 50.725 69 34 0 212 280 54 122 1.20e-16 80.1
MS.gene85293.t1 MTR_1g070455 52.459 61 29 0 212 272 294 354 1.34e-16 81.3
MS.gene85293.t1 MTR_1g009613 52.542 59 28 0 212 270 263 321 6.05e-16 79.0
MS.gene85293.t1 MTR_2g083870 55.000 60 27 0 212 271 81 140 7.56e-16 77.4
MS.gene85293.t1 MTR_8g007270 45.882 85 41 2 188 268 127 210 1.01e-15 76.6
MS.gene85293.t1 MTR_2g032030 39.394 99 59 1 186 283 241 339 1.41e-15 79.0
MS.gene85293.t1 MTR_1g099600 43.678 87 49 0 211 297 185 271 1.61e-15 77.4
MS.gene85293.t1 MTR_3g095080 41.053 95 53 2 195 289 128 219 1.80e-15 75.5
MS.gene85293.t1 MTR_2g032030 39.394 99 59 1 186 283 241 339 1.95e-15 78.2
MS.gene85293.t1 MTR_8g028565 51.429 70 34 0 212 281 156 225 2.43e-15 77.0
MS.gene85293.t1 MTR_3g095080 40.625 96 54 2 194 289 151 243 2.47e-15 75.9
MS.gene85293.t1 MTR_8g098945 42.353 85 47 1 210 294 128 210 8.40e-15 74.7
MS.gene85293.t1 MTR_7g079040 50.000 70 35 0 212 281 155 224 1.53e-14 74.7
MS.gene85293.t1 MTR_0117s0040 49.153 59 30 0 211 269 74 132 1.86e-14 73.2
MS.gene85293.t1 MTR_4g127040 52.632 57 27 0 212 268 246 302 2.48e-14 73.9
MS.gene85293.t1 MTR_8g092140 48.438 64 32 1 205 268 154 216 9.65e-14 70.9
MS.gene85293.t1 MTR_3g009470 39.362 94 54 3 183 275 251 342 1.24e-13 73.2
MS.gene85293.t1 MTR_1g078260 28.272 191 127 5 79 268 123 304 1.26e-13 72.8
MS.gene85293.t1 MTR_3g108960 39.744 78 47 0 195 272 298 375 1.43e-13 72.4
MS.gene85293.t1 MTR_6g086555 52.308 65 29 2 212 275 207 270 5.46e-13 70.5
MS.gene85293.t1 MTR_4g007060 44.444 63 35 0 186 248 137 199 5.87e-13 68.6
MS.gene85293.t1 MTR_7g062220 52.632 57 26 1 212 268 125 180 8.73e-13 69.3
MS.gene85293.t1 MTR_8g069935 41.667 60 35 0 212 271 332 391 3.34e-12 68.6
MS.gene85293.t1 MTR_6g053200 41.667 60 35 0 212 271 331 390 3.69e-12 68.6
MS.gene85293.t1 MTR_3g031220 47.059 68 29 3 212 279 199 259 4.66e-12 67.8
MS.gene85293.t1 MTR_8g067650 45.000 60 33 0 212 271 340 399 5.35e-12 67.8
MS.gene85293.t1 MTR_8g067635 45.000 60 33 0 212 271 350 409 8.33e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85293.t1 AT4G04450 37.942 311 143 7 70 338 20 322 2.78e-53 184
MS.gene85293.t1 AT4G04450 37.942 311 143 7 70 338 114 416 2.75e-52 183
MS.gene85293.t1 AT1G62300 36.070 402 174 17 74 411 158 540 4.34e-49 175
MS.gene85293.t1 AT4G01720 34.324 370 192 9 66 406 96 443 6.69e-46 166
MS.gene85293.t1 AT4G22070 35.494 324 153 9 66 341 108 423 3.36e-43 159
MS.gene85293.t1 AT4G22070 35.494 324 153 9 66 341 108 423 3.36e-43 159
MS.gene85293.t1 AT4G22070 35.494 324 153 9 66 341 108 423 3.36e-43 159
MS.gene85293.t1 AT4G22070 35.494 324 153 9 66 341 108 423 3.36e-43 159
MS.gene85293.t1 AT5G15130 34.431 334 144 9 68 340 35 354 1.18e-41 155
MS.gene85293.t1 AT5G15130 51.534 163 63 4 187 340 157 312 1.68e-41 154
MS.gene85293.t1 AT1G68150 64.815 108 37 1 189 296 213 319 5.46e-41 150
MS.gene85293.t1 AT1G18860 33.524 349 159 10 68 343 41 389 5.64e-37 142
MS.gene85293.t1 AT1G69810 44.937 158 74 3 185 340 177 323 1.17e-32 127
MS.gene85293.t1 AT1G69810 44.172 163 78 3 180 340 114 265 1.31e-32 126
MS.gene85293.t1 AT5G07100 39.130 138 73 4 146 280 81 210 4.07e-25 102
MS.gene85293.t1 AT5G07100 39.130 138 73 4 146 280 81 210 4.07e-25 102
MS.gene85293.t1 AT5G07100 39.130 138 73 4 146 280 81 210 4.07e-25 102
MS.gene85293.t1 AT5G07100 39.130 138 73 4 146 280 81 210 4.07e-25 102
MS.gene85293.t1 AT5G07100 38.571 140 75 4 144 280 172 303 6.20e-25 104
MS.gene85293.t1 AT1G80840 31.658 199 106 6 75 271 36 206 4.05e-22 96.3
MS.gene85293.t1 AT4G31800 56.000 75 32 1 205 278 104 178 6.38e-22 94.7
MS.gene85293.t1 AT4G31800 56.000 75 32 1 205 278 169 243 6.71e-22 95.9
MS.gene85293.t1 AT2G38470 42.857 119 64 3 156 273 308 423 8.02e-22 98.2
MS.gene85293.t1 AT2G38470 48.333 60 29 2 212 271 185 242 5.27e-11 65.1
MS.gene85293.t1 AT2G47260 48.810 84 42 1 195 278 158 240 1.26e-21 95.5
MS.gene85293.t1 AT1G29860 48.148 81 41 1 195 275 120 199 2.02e-21 94.0
MS.gene85293.t1 AT5G41570 48.352 91 42 2 182 268 65 154 2.46e-21 91.3
MS.gene85293.t1 AT2G30250 47.826 92 45 2 186 275 301 391 3.75e-21 95.1
MS.gene85293.t1 AT2G04880 49.351 77 38 1 197 273 269 344 5.30e-21 95.5
MS.gene85293.t1 AT2G25000 55.556 72 31 1 204 274 95 166 6.14e-21 91.3
MS.gene85293.t1 AT2G25000 55.556 72 31 1 204 274 95 166 6.14e-21 91.3
MS.gene85293.t1 AT2G04880 49.351 77 38 1 197 273 293 368 7.09e-21 95.1
MS.gene85293.t1 AT2G25000 55.556 72 31 1 204 274 117 188 1.01e-20 91.3
MS.gene85293.t1 AT4G18170 46.875 96 48 3 178 271 137 231 1.15e-20 92.4
MS.gene85293.t1 AT2G25000 55.556 72 31 1 204 274 138 209 1.19e-20 91.7
MS.gene85293.t1 AT1G64000 51.190 84 37 2 198 281 101 180 2.92e-20 88.6
MS.gene85293.t1 AT5G56270 48.864 88 44 1 186 273 462 548 4.51e-20 93.2
MS.gene85293.t1 AT4G26640 49.351 77 38 1 195 271 189 264 4.80e-20 91.7
MS.gene85293.t1 AT4G26640 49.351 77 38 1 195 271 189 264 4.80e-20 91.7
MS.gene85293.t1 AT4G26640 49.351 77 38 1 195 271 189 264 4.80e-20 91.7
MS.gene85293.t1 AT4G26640 49.351 77 38 1 195 271 189 264 4.80e-20 91.7
MS.gene85293.t1 AT4G26640 48.718 78 39 1 194 271 324 400 7.78e-20 92.0
MS.gene85293.t1 AT4G26640 49.351 77 38 1 195 271 293 368 9.09e-20 91.7
MS.gene85293.t1 AT4G26640 49.351 77 38 1 195 271 365 440 1.05e-19 92.0
MS.gene85293.t1 AT5G26170 52.778 72 33 1 197 268 99 169 1.41e-19 86.3
MS.gene85293.t1 AT1G13960 50.000 74 36 1 198 271 243 315 1.80e-19 89.7
MS.gene85293.t1 AT1G13960 50.000 60 28 2 212 271 77 134 1.28e-11 66.2
MS.gene85293.t1 AT1G13960 50.000 74 36 1 198 271 396 468 2.57e-19 90.5
MS.gene85293.t1 AT1G13960 50.000 60 28 2 212 271 230 287 9.37e-12 67.4
MS.gene85293.t1 AT3G62340 44.037 109 55 2 198 301 105 212 4.08e-19 87.4
MS.gene85293.t1 AT5G64810 48.276 87 41 2 183 269 85 167 4.36e-19 85.5
MS.gene85293.t1 AT2G03340 48.684 76 38 1 198 273 402 476 4.90e-19 89.7
MS.gene85293.t1 AT2G03340 51.667 60 27 2 212 271 251 308 9.04e-13 70.5
MS.gene85293.t1 AT2G46130 45.263 95 48 2 187 281 6 96 4.97e-19 82.4
MS.gene85293.t1 AT5G13080 48.193 83 39 2 198 280 54 132 4.98e-19 83.6
MS.gene85293.t1 AT5G49520 45.161 93 49 2 195 286 205 296 5.11e-19 89.0
MS.gene85293.t1 AT5G46350 41.667 96 54 2 198 293 170 263 5.22e-19 87.8
MS.gene85293.t1 AT3G01970 50.000 74 36 1 197 270 51 123 1.25e-18 82.8
MS.gene85293.t1 AT3G01080 48.649 74 37 1 198 271 293 365 1.51e-18 87.8
MS.gene85293.t1 AT3G01080 48.649 74 37 1 198 271 331 403 1.73e-18 87.8
MS.gene85293.t1 AT1G69310 45.238 84 45 1 186 269 122 204 2.32e-18 85.5
MS.gene85293.t1 AT1G69310 45.238 84 45 1 186 269 122 204 2.32e-18 85.5
MS.gene85293.t1 AT1G69310 45.238 84 45 1 186 269 122 204 2.32e-18 85.5
MS.gene85293.t1 AT4G26440 34.459 148 83 5 161 295 311 457 5.36e-18 86.7
MS.gene85293.t1 AT4G30935 38.136 118 67 3 182 295 303 418 6.60e-18 85.9
MS.gene85293.t1 AT2G37260 39.695 131 59 6 157 274 209 332 1.70e-17 84.0
MS.gene85293.t1 AT2G37260 44.156 77 39 3 211 287 83 155 3.06e-13 70.9
MS.gene85293.t1 AT2G37260 39.695 131 59 6 157 274 211 334 1.75e-17 84.0
MS.gene85293.t1 AT2G37260 44.156 77 39 3 211 287 85 157 3.25e-13 70.9
MS.gene85293.t1 AT2G23320 57.627 59 25 0 212 270 241 299 2.65e-17 82.8
MS.gene85293.t1 AT2G37260 41.221 131 57 7 157 274 291 414 2.91e-17 84.0
MS.gene85293.t1 AT2G37260 44.156 77 39 3 211 287 165 237 4.31e-13 71.2
MS.gene85293.t1 AT2G30590 54.688 64 28 1 212 274 314 377 6.89e-17 82.4
MS.gene85293.t1 AT5G28650 57.895 57 24 0 212 268 263 319 7.60e-17 81.6
MS.gene85293.t1 AT3G04670 57.895 57 24 0 212 268 263 319 1.18e-16 81.3
MS.gene85293.t1 AT4G24240 55.932 59 26 0 212 270 282 340 2.33e-16 80.5
MS.gene85293.t1 AT1G55600 42.697 89 46 2 189 273 281 368 2.81e-16 81.3
MS.gene85293.t1 AT1G29280 45.882 85 38 1 202 286 65 141 3.66e-16 78.6
MS.gene85293.t1 AT4G31550 44.872 78 40 1 212 289 247 321 4.07e-16 79.3
MS.gene85293.t1 AT4G31550 44.872 78 40 1 212 289 246 320 4.22e-16 79.3
MS.gene85293.t1 AT2G44745 43.956 91 46 2 182 268 112 201 4.35e-16 77.4
MS.gene85293.t1 AT2G24570 53.448 58 27 0 212 269 244 301 1.07e-15 78.2
MS.gene85293.t1 AT1G30650 43.529 85 47 1 188 271 193 277 1.64e-15 78.6
MS.gene85293.t1 AT2G34830 43.529 85 47 1 188 271 191 275 1.77e-15 78.6
MS.gene85293.t1 AT4G01250 48.649 74 38 0 208 281 125 198 1.88e-15 77.0
MS.gene85293.t1 AT2G34830 43.529 85 47 1 188 271 200 284 2.25e-15 78.2
MS.gene85293.t1 AT4G23550 43.373 83 47 0 209 291 138 220 2.79e-15 76.6
MS.gene85293.t1 AT3G58710 55.000 60 27 0 212 271 72 131 4.28e-15 75.5
MS.gene85293.t1 AT3G58710 55.000 60 27 0 212 271 71 130 4.56e-15 75.5
MS.gene85293.t1 AT5G52830 50.000 70 35 0 212 281 166 235 1.42e-14 75.1
MS.gene85293.t1 AT2G46130 42.105 95 47 3 187 281 6 92 3.42e-14 68.9
MS.gene85293.t1 AT4G39410 40.000 85 50 1 184 268 196 279 5.62e-14 72.8
MS.gene85293.t1 AT5G45050 36.842 114 69 1 212 322 1042 1155 1.34e-13 73.6
MS.gene85293.t1 AT5G45050 36.842 114 69 1 212 322 1153 1266 1.46e-13 73.6
MS.gene85293.t1 AT5G45050 36.842 114 69 1 212 322 1181 1294 1.50e-13 73.6
MS.gene85293.t1 AT1G69310 41.667 84 44 2 186 269 122 200 1.52e-13 71.2
MS.gene85293.t1 AT5G43290 47.541 61 31 1 212 272 115 174 3.26e-12 67.0
MS.gene85293.t1 AT4G31800 62.222 45 16 1 205 248 169 213 4.30e-12 66.2
MS.gene85293.t1 AT2G21900 46.667 60 31 1 212 271 110 168 5.18e-12 65.1
MS.gene85293.t1 AT2G46400 36.000 100 62 1 190 287 83 182 3.74e-11 64.3
MS.gene85293.t1 AT2G40740 43.902 82 35 3 212 282 174 255 4.15e-11 63.9
MS.gene85293.t1 AT2G40740 43.902 82 35 3 212 282 183 264 5.47e-11 63.9

Find 96 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCATTGTTTATCATATT+TGG 0.055350 2.4:+45272997 None:intergenic
CACTACTCTTACTAGGTAAT+TGG 0.189983 2.4:+45272851 None:intergenic
ATTAATCACTTACTTGGATT+TGG 0.221328 2.4:+45276126 None:intergenic
TTAATCACTTACTTGGATTT+GGG 0.256367 2.4:+45276127 None:intergenic
GAAAACTTACAGTATTCTTA+TGG 0.285405 2.4:+45276586 None:intergenic
TCTTCAGAATCGTTTACTTT+TGG 0.317553 2.4:-45275809 MS.gene85293:CDS
AGCCTACTATCGTTGCAGTA+TGG 0.326812 2.4:-45274857 MS.gene85293:CDS
GGGGATTTCCTTGAATTTGA+TGG 0.332291 2.4:+45276147 None:intergenic
AAAATCTGATCGTGCCCTTA+TGG 0.343745 2.4:+45275063 None:intergenic
GGGTGTCAATGGAGAAAATA+TGG 0.346609 2.4:-45274913 MS.gene85293:CDS
TCTTTATTGTTATTATCATA+TGG 0.359665 2.4:+45276642 None:intergenic
TAGTTTACCTGCTTACGAAC+TGG 0.359755 2.4:+45274823 None:intergenic
GAAGTTGTGTTGTGTTATTA+AGG 0.369494 2.4:+45276171 None:intergenic
GATGATGTTAGGTTTGCTAT+TGG 0.371407 2.4:+45273285 None:intergenic
TCTTACTAGGTAATTGGCTT+TGG 0.372010 2.4:+45272857 None:intergenic
TTTGAGTTTGGTGGAGATAA+TGG 0.378048 2.4:+45273177 None:intergenic
AATTAACCACTACTCTTACT+AGG 0.379801 2.4:+45272844 None:intergenic
GTAGCTATAACATTTGAGTT+TGG 0.383227 2.4:+45273165 None:intergenic
CTTAAGAGGTATGCTAAATC+TGG 0.384344 2.4:-45275851 MS.gene85293:CDS
TCAACAAATCTACAACAATA+TGG 0.389536 2.4:-45273234 MS.gene85293:CDS
ATGATTATGGCTCCCTTCAT+AGG 0.402020 2.4:+45273329 None:intergenic
CAATGAGAGAAAACTTGCTT+TGG 0.409723 2.4:-45272983 MS.gene85293:CDS
AGTTCAAACAATGAAATTGC+TGG 0.417191 2.4:-45276682 MS.gene85293:CDS
CGATAGTAGGCTCGTGGACA+AGG 0.430122 2.4:+45274868 None:intergenic
TATTGAGTGCTGATGATGTT+AGG 0.434026 2.4:+45273274 None:intergenic
GTAAAATCAAGTGTAATTGT+AGG 0.435039 2.4:+45273120 None:intergenic
TGTAATGTTTAGATGGTTGA+TGG 0.440145 2.4:-45274934 MS.gene85293:intron
GGAGAGCTAGAGTGTCCATA+AGG 0.442520 2.4:-45275078 MS.gene85293:CDS
GGGATTTCCTTGAATTTGAT+GGG 0.451310 2.4:+45276148 None:intergenic
AAGGAGAATGAAAACTTAAG+AGG 0.454898 2.4:-45275865 MS.gene85293:CDS
GCTATAACATTTGAGTTTGG+TGG 0.456982 2.4:+45273168 None:intergenic
AAATAATGGATTTGCCATAG+TGG 0.457421 2.4:-45275203 MS.gene85293:CDS
TTGAATTGTAAATAGTTACT+TGG 0.461039 2.4:+45273090 None:intergenic
CTGGTGGAAGTTGATGATTA+TGG 0.462621 2.4:+45273316 None:intergenic
TGAAATAAATAGCAAAATAG+AGG 0.463782 2.4:-45275125 MS.gene85293:CDS
TAATGACCTTGGAATGGAAA+AGG 0.467036 2.4:+45273042 None:intergenic
CAACTTCCCCATCAAATTCA+AGG 0.467550 2.4:-45276155 MS.gene85293:CDS
AGATGGTTGATGGGTGTCAA+TGG 0.469133 2.4:-45274924 MS.gene85293:CDS
GCCTACTATCGTTGCAGTAT+GGG 0.470435 2.4:-45274856 MS.gene85293:CDS
TGCACCCAACCTCAGATGAT+TGG 0.470879 2.4:+45275101 None:intergenic
ACAGGTGCAAAGGTGTTTCA+AGG 0.472939 2.4:-45273373 MS.gene85293:intron
GCCATGTCCTCGTTCACAAA+TGG 0.479556 2.4:-45272901 MS.gene85293:CDS
TTTGGAAACACCTCAAATGA+TGG 0.495575 2.4:+45273015 None:intergenic
CCCCATACTGCAACGATAGT+AGG 0.503349 2.4:+45274855 None:intergenic
GAAGCTTGATGAAGCAAAGA+AGG 0.508309 2.4:-45275884 MS.gene85293:CDS
AGGACCAATCATCTGAGGTT+GGG 0.511884 2.4:-45275105 MS.gene85293:CDS
AGTTTACCTGCTTACGAACT+GGG 0.512250 2.4:+45274824 None:intergenic
GAGAGCTAGAGTGTCCATAA+GGG 0.513998 2.4:-45275077 MS.gene85293:CDS
TTCATGCCCAGTTCGTAAGC+AGG 0.514284 2.4:-45274830 MS.gene85293:intron
CTTAATAAAAGAGTAGATCC+TGG 0.516100 2.4:+45276200 None:intergenic
GTGTTCATCACAACCTATGA+AGG 0.519912 2.4:-45273342 MS.gene85293:CDS
AGTATTCTTATGGAAGGAAG+AGG 0.520852 2.4:+45276596 None:intergenic
TAAAATCAAGTGTAATTGTA+GGG 0.521053 2.4:+45273121 None:intergenic
AAATAATCACAACTTGCTGC+TGG 0.523498 2.4:-45275752 MS.gene85293:intron
GAGGACCAATCATCTGAGGT+TGG 0.528239 2.4:-45275106 MS.gene85293:CDS
TCGTGGACAAGGGTTGCCCT+TGG 0.531338 2.4:+45274879 None:intergenic
ATTGTAGGGCATGTTGGAGA+TGG 0.532259 2.4:+45273135 None:intergenic
AGTGTAATTGTAGGGCATGT+TGG 0.533130 2.4:+45273129 None:intergenic
AATCATCTGAGGTTGGGTGC+AGG 0.533609 2.4:-45275099 MS.gene85293:CDS
AATTGTAAATAGTTACTTGG+AGG 0.535746 2.4:+45273093 None:intergenic
TTGCTACAATCTGAAGGTGA+AGG 0.539687 2.4:+45275226 None:intergenic
GAGATAATGGTGAAGAGAAG+AGG 0.540861 2.4:+45273190 None:intergenic
TCATTACCCTCCATCATTTG+AGG 0.541576 2.4:-45273025 MS.gene85293:CDS
ATGATGGAGGGTAATGACCT+TGG 0.552017 2.4:+45273031 None:intergenic
GCATGTGAGTACATTAATGA+AGG 0.552654 2.4:-45275148 MS.gene85293:CDS
TCCATTTGTGAACGAGGACA+TGG 0.553076 2.4:+45272900 None:intergenic
ATTGTAAATAGTTACTTGGA+GGG 0.553408 2.4:+45273094 None:intergenic
GTAATGTTTAGATGGTTGAT+GGG 0.564516 2.4:-45274933 MS.gene85293:intron
TGTAGATTTGTTGATGAGGT+AGG 0.565582 2.4:+45273243 None:intergenic
GATTTGTTGATGAGGTAGGA+AGG 0.569280 2.4:+45273247 None:intergenic
AACTGACGAGTAGGTAACAC+TGG 0.572998 2.4:+45275259 None:intergenic
ACTTACAGTATTCTTATGGA+AGG 0.574288 2.4:+45276590 None:intergenic
ATTGCAATTAACAGGTGCAA+AGG 0.574633 2.4:-45273383 MS.gene85293:intron
AATGACCTTGGAATGGAAAA+GGG 0.575264 2.4:+45273043 None:intergenic
TAATCACTTACTTGGATTTG+GGG 0.580090 2.4:+45276128 None:intergenic
AATAATGCTTCCTTGAGAGA+AGG 0.583998 2.4:+45272928 None:intergenic
CCTACTATCGTTGCAGTATG+GGG 0.587972 2.4:-45274855 MS.gene85293:CDS
TTATTGTTATTATCATATGG+TGG 0.589100 2.4:+45276645 None:intergenic
GGAAACACCTCAAATGATGG+AGG 0.600501 2.4:+45273018 None:intergenic
GGAGGGTAATGACCTTGGAA+TGG 0.607233 2.4:+45273036 None:intergenic
GAAGGGAAATACACAAGATG+CGG 0.615352 2.4:-45275287 MS.gene85293:CDS
TCATCAAAGAACTGACGAGT+AGG 0.619422 2.4:+45275250 None:intergenic
TATGGACAAAAGACAGCCAA+GGG 0.623915 2.4:-45274895 MS.gene85293:CDS
ATATGGACAAAAGACAGCCA+AGG 0.629423 2.4:-45274896 MS.gene85293:CDS
GAAACACCTCAAATGATGGA+GGG 0.632932 2.4:+45273019 None:intergenic
CTGCAACGATAGTAGGCTCG+TGG 0.637106 2.4:+45274862 None:intergenic
CAATTACCTAGTAAGAGTAG+TGG 0.638666 2.4:-45272850 MS.gene85293:CDS
GATAGTAGGCTCGTGGACAA+GGG 0.644110 2.4:+45274869 None:intergenic
GGAAAACAATGAAAACAACA+TGG 0.645971 2.4:-45275182 MS.gene85293:CDS
TGTTCATCACAACCTATGAA+GGG 0.646804 2.4:-45273341 MS.gene85293:CDS
GGATTTCCTTGAATTTGATG+GGG 0.651214 2.4:+45276149 None:intergenic
AATAGAGGACCAATCATCTG+AGG 0.655874 2.4:-45275110 MS.gene85293:CDS
AGAATCTCCATTTGTGAACG+AGG 0.664014 2.4:+45272894 None:intergenic
GAAAACAATGAAAACAACAT+GGG 0.672904 2.4:-45275181 MS.gene85293:CDS
TTGTTGTAGATTTGTTGATG+AGG 0.675654 2.4:+45273239 None:intergenic
AATTGCTGGAAGTTGATGAA+AGG 0.676322 2.4:-45276668 MS.gene85293:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAATCAAATATCAAATA+TGG + chr2.4:45273013-45273032 None:intergenic 10.0%
!!! AATAACATTTTTTAGAATTT+AGG + chr2.4:45274902-45274921 None:intergenic 10.0%
!!! ATCTATTTTCAAATATTATA+AGG - chr2.4:45275612-45275631 MS.gene85293:intron 10.0%
!!! TCTATTTTCAAATATTATAA+GGG - chr2.4:45275613-45275632 MS.gene85293:intron 10.0%
!!! TTTTATATGTATAATAGAAA+TGG - chr2.4:45275084-45275103 MS.gene85293:CDS 10.0%
!! CAAAATATAAGAATTTCAAT+TGG - chr2.4:45274854-45274873 MS.gene85293:CDS 15.0%
!! TTAAATTTCTATCAAAGATA+TGG - chr2.4:45275319-45275338 MS.gene85293:intron 15.0%
!! TTCTTCATAATTAATTAGAA+GGG - chr2.4:45274235-45274254 MS.gene85293:intron 15.0%
!! TTTCTTCATAATTAATTAGA+AGG - chr2.4:45274234-45274253 MS.gene85293:intron 15.0%
!!! AAAAGTTTTTGTAATGTAAT+TGG + chr2.4:45275266-45275285 None:intergenic 15.0%
!!! AATTTTAATGCATTGAAAAA+GGG - chr2.4:45275207-45275226 MS.gene85293:CDS 15.0%
!!! ATTAATTTGTAATGTTTAGA+TGG - chr2.4:45274598-45274617 MS.gene85293:intron 15.0%
!!! ATTTTCAAATATTTCGAAAA+TGG - chr2.4:45275584-45275603 MS.gene85293:intron 15.0%
!!! ATTTTTGTTTTTTATGTTTG+TGG - chr2.4:45274936-45274955 MS.gene85293:intron 15.0%
!!! GATAAATTAAACTCTAATTT+TGG - chr2.4:45274120-45274139 MS.gene85293:intron 15.0%
!!! TCTTTATTGTTATTATCATA+TGG + chr2.4:45272900-45272919 None:intergenic 15.0%
!!! TTTTCAAATATTTCGAAAAT+GGG - chr2.4:45275585-45275604 MS.gene85293:intron 15.0%
!! AATAAGATTAATCACTTACT+TGG + chr2.4:45273422-45273441 None:intergenic 20.0%
!! ATTTAATGATCTCATGTATT+TGG - chr2.4:45273891-45273910 MS.gene85293:intron 20.0%
!! TAAAATCAAGTGTAATTGTA+GGG + chr2.4:45276421-45276440 None:intergenic 20.0%
!! TAACCGAACAATAAATAAAA+TGG - chr2.4:45275468-45275487 MS.gene85293:intron 20.0%
!! TAGGTTATATCATATCAAAA+TGG + chr2.4:45274764-45274783 None:intergenic 20.0%
!! TATGAAGAAAAAATGTACAA+AGG + chr2.4:45274224-45274243 None:intergenic 20.0%
!! TGAAATAAATAGCAAAATAG+AGG - chr2.4:45274414-45274433 MS.gene85293:intron 20.0%
!! TTAATTACATTGCAATTAAC+AGG - chr2.4:45276148-45276167 MS.gene85293:CDS 20.0%
!! TTGAATTGTAAATAGTTACT+TGG + chr2.4:45276452-45276471 None:intergenic 20.0%
!! TTTATCTATTTGTAAGTAGA+CGG + chr2.4:45273970-45273989 None:intergenic 20.0%
!! TTTCAAATATTTCGAAAATG+GGG - chr2.4:45275586-45275605 MS.gene85293:intron 20.0%
!!! AATTCTCATTTCATTCTTTT+AGG + chr2.4:45275814-45275833 None:intergenic 20.0%
!!! ACATTTTTTAGAATTTAGGT+TGG + chr2.4:45274898-45274917 None:intergenic 20.0%
!!! ATAATGATGAGACTATTTTA+AGG - chr2.4:45276040-45276059 MS.gene85293:intron 20.0%
!!! ATTTTAATGCATTGAAAAAG+GGG - chr2.4:45275208-45275227 MS.gene85293:CDS 20.0%
!!! CAATTTTAATGCATTGAAAA+AGG - chr2.4:45275206-45275225 MS.gene85293:CDS 20.0%
!!! CATTTTTTAGAATTTAGGTT+GGG + chr2.4:45274897-45274916 None:intergenic 20.0%
!!! CTTCCATTTTATTTATTGTT+CGG + chr2.4:45275474-45275493 None:intergenic 20.0%
!!! TAATAACTTGACATTTTGAT+GGG - chr2.4:45273304-45273323 MS.gene85293:CDS 20.0%
!!! TGTGTAATTCTGTTTTAAAA+TGG - chr2.4:45276076-45276095 MS.gene85293:intron 20.0%
!!! TGTTGTTTATGTAATTAACA+TGG - chr2.4:45274046-45274065 MS.gene85293:intron 20.0%
!!! TTAAACTCTAATTTTGGATT+TGG - chr2.4:45274126-45274145 MS.gene85293:intron 20.0%
!!! TTACAAGTTGAAAATATCAT+AGG + chr2.4:45274179-45274198 None:intergenic 20.0%
!!! TTATTGTTATTATCATATGG+TGG + chr2.4:45272897-45272916 None:intergenic 20.0%
! AAAATTCAAGAACCAACTAT+TGG + chr2.4:45275873-45275892 None:intergenic 25.0%
! AAAGTCATGAATACATCATA+AGG - chr2.4:45276016-45276035 MS.gene85293:intron 25.0%
! AAATGAGTGTGAATCAAATA+AGG + chr2.4:45273844-45273863 None:intergenic 25.0%
! AAATGTCAAGTTATTAGGTT+AGG + chr2.4:45273300-45273319 None:intergenic 25.0%
! AATTGTAAATAGTTACTTGG+AGG + chr2.4:45276449-45276468 None:intergenic 25.0%
! ATGTAATGAATGTTTGTGAA+AGG - chr2.4:45275498-45275517 MS.gene85293:intron 25.0%
! ATTAATCACTTACTTGGATT+TGG + chr2.4:45273416-45273435 None:intergenic 25.0%
! ATTATAAGGAAGATGATGAT+GGG - chr2.4:45275744-45275763 MS.gene85293:intron 25.0%
! ATTGTAAATAGTTACTTGGA+GGG + chr2.4:45276448-45276467 None:intergenic 25.0%
! CATCAAAATGTCAAGTTATT+AGG + chr2.4:45273305-45273324 None:intergenic 25.0%
! CTCTCATTGTTTATCATATT+TGG + chr2.4:45276545-45276564 None:intergenic 25.0%
! GAAAACAATGAAAACAACAT+GGG - chr2.4:45274358-45274377 MS.gene85293:intron 25.0%
! GAAAACTTACAGTATTCTTA+TGG + chr2.4:45272956-45272975 None:intergenic 25.0%
! GTAAAATCAAGTGTAATTGT+AGG + chr2.4:45276422-45276441 None:intergenic 25.0%
! TCAACAAATCTACAACAATA+TGG - chr2.4:45276305-45276324 MS.gene85293:intron 25.0%
! TCAGATTGTAGCAAAAATAA+TGG - chr2.4:45274322-45274341 MS.gene85293:intron 25.0%
! TTAATCACTTACTTGGATTT+GGG + chr2.4:45273415-45273434 None:intergenic 25.0%
! TTATCTATTTGTAAGTAGAC+GGG + chr2.4:45273969-45273988 None:intergenic 25.0%
! TTCCTTATAATGCTAATTAG+TGG + chr2.4:45275735-45275754 None:intergenic 25.0%
!! AAGAGTCATATACTTTGTTT+AGG - chr2.4:45273103-45273122 MS.gene85293:CDS 25.0%
!! CTAATAACTTGACATTTTGA+TGG - chr2.4:45273303-45273322 MS.gene85293:CDS 25.0%
!! GTTCAATTGATAATATAGCA+AGG - chr2.4:45275380-45275399 MS.gene85293:intron 25.0%
!! TCGAACTTATGATAGTTTTT+AGG + chr2.4:45274783-45274802 None:intergenic 25.0%
!!! TTATTTTTGCTACAATCTGA+AGG + chr2.4:45274322-45274341 None:intergenic 25.0%
!!! TTTGATAATTGCTCTAAGAT+TGG - chr2.4:45273620-45273639 MS.gene85293:intron 25.0%
AAGGAGAATGAAAACTTAAG+AGG - chr2.4:45273674-45273693 MS.gene85293:intron 30.0%
ACTTACAGTATTCTTATGGA+AGG + chr2.4:45272952-45272971 None:intergenic 30.0%
AGTTCAAACAATGAAATTGC+TGG - chr2.4:45272857-45272876 MS.gene85293:CDS 30.0%
ATGAATGTTTGTGAAAGGAA+AGG - chr2.4:45275503-45275522 MS.gene85293:intron 30.0%
ATTGATTATCCTCCAATAGT+TGG - chr2.4:45275858-45275877 MS.gene85293:CDS 30.0%
CATTATAAGGAAGATGATGA+TGG - chr2.4:45275743-45275762 MS.gene85293:intron 30.0%
CTTAATAAAAGAGTAGATCC+TGG + chr2.4:45273342-45273361 None:intergenic 30.0%
GGAAAACAATGAAAACAACA+TGG - chr2.4:45274357-45274376 MS.gene85293:intron 30.0%
GTAATGTTTAGATGGTTGAT+GGG - chr2.4:45274606-45274625 MS.gene85293:intron 30.0%
TAATCACTTACTTGGATTTG+GGG + chr2.4:45273414-45273433 None:intergenic 30.0%
TAATCCAGTATACACAAACA+TGG + chr2.4:45272986-45273005 None:intergenic 30.0%
TAGCTTATGATGAAAATCGT+GGG - chr2.4:45273211-45273230 MS.gene85293:CDS 30.0%
TGCCACTAATTAGCATTATA+AGG - chr2.4:45275730-45275749 MS.gene85293:intron 30.0%
TGTAATGTTTAGATGGTTGA+TGG - chr2.4:45274605-45274624 MS.gene85293:intron 30.0%
TTAGCTTATGATGAAAATCG+TGG - chr2.4:45273210-45273229 MS.gene85293:CDS 30.0%
TTGTAGTGCATCATCAATTA+AGG + chr2.4:45275173-45275192 None:intergenic 30.0%
! ATTGATAATATAGCAAGGTC+TGG - chr2.4:45275385-45275404 MS.gene85293:intron 30.0%
! CGGACAAACTATTAATTTGA+GGG - chr2.4:45274996-45275015 MS.gene85293:intron 30.0%
! GAAGTTGTGTTGTGTTATTA+AGG + chr2.4:45273371-45273390 None:intergenic 30.0%
! GACTATTAGTTCTTACTAGA+TGG - chr2.4:45275344-45275363 MS.gene85293:intron 30.0%
! GATCAGATTTTGCATTTGTA+AGG - chr2.4:45274488-45274507 MS.gene85293:intron 30.0%
! GGACAAACTATTAATTTGAG+GGG - chr2.4:45274997-45275016 MS.gene85293:intron 30.0%
! GGGTATTGTTTCTCATATTA+GGG - chr2.4:45275633-45275652 MS.gene85293:intron 30.0%
! GTAAATGAGACAAGTTTTCA+TGG + chr2.4:45275972-45275991 None:intergenic 30.0%
! GTAGCTATAACATTTGAGTT+TGG + chr2.4:45276377-45276396 None:intergenic 30.0%
! GTTTTCATTGTTTTCCACTA+TGG + chr2.4:45274353-45274372 None:intergenic 30.0%
! TATTAGTTCTTACTAGATGG+AGG - chr2.4:45275347-45275366 MS.gene85293:intron 30.0%
! TCGATTTTATAGAAGTGAGT+TGG - chr2.4:45274823-45274842 MS.gene85293:intron 30.0%
! TCTTCAGAATCGTTTACTTT+TGG - chr2.4:45273730-45273749 MS.gene85293:intron 30.0%
! TTGATAATATAGCAAGGTCT+GGG - chr2.4:45275386-45275405 MS.gene85293:intron 30.0%
!! AAATAATGGATTTGCCATAG+TGG - chr2.4:45274336-45274355 MS.gene85293:intron 30.0%
!! AGCAAGTTGTGATTATTTTG+AGG + chr2.4:45273785-45273804 None:intergenic 30.0%
!! AGGGTATTGTTTCTCATATT+AGG - chr2.4:45275632-45275651 MS.gene85293:intron 30.0%
!! TGTTCTAAAGACACTCTTTA+TGG - chr2.4:45275670-45275689 MS.gene85293:intron 30.0%
!! TTGTTGTAGATTTGTTGATG+AGG + chr2.4:45276303-45276322 None:intergenic 30.0%
!!! AATTTTGGATTTGGTGAATG+TGG - chr2.4:45274135-45274154 MS.gene85293:intron 30.0%
!!! TTCTTTTAGGTTGAATGTGT+AGG + chr2.4:45275801-45275820 None:intergenic 30.0%
AACACAACCAAAAAAGATGG+AGG - chr2.4:45275420-45275439 MS.gene85293:intron 35.0%
AATAATGCTTCCTTGAGAGA+AGG + chr2.4:45276614-45276633 None:intergenic 35.0%
AATGACCTTGGAATGGAAAA+GGG + chr2.4:45276499-45276518 None:intergenic 35.0%
AATGTTTGTGAAAGGAAAGG+TGG - chr2.4:45275506-45275525 MS.gene85293:intron 35.0%
AGAAAAAGATCCTTCTCTCA+AGG - chr2.4:45276601-45276620 MS.gene85293:CDS 35.0%
AGTATTCTTATGGAAGGAAG+AGG + chr2.4:45272946-45272965 None:intergenic 35.0%
ATGTTTGTGAAAGGAAAGGT+GGG - chr2.4:45275507-45275526 MS.gene85293:intron 35.0%
ATTCAAGAACCAACTATTGG+AGG + chr2.4:45275870-45275889 None:intergenic 35.0%
ATTGCAATTAACAGGTGCAA+AGG - chr2.4:45276156-45276175 MS.gene85293:CDS 35.0%
CAATTACCTAGTAAGAGTAG+TGG - chr2.4:45276689-45276708 MS.gene85293:CDS 35.0%
CACTACTCTTACTAGGTAAT+TGG + chr2.4:45276691-45276710 None:intergenic 35.0%
GCATGTGAGTACATTAATGA+AGG - chr2.4:45274391-45274410 MS.gene85293:intron 35.0%
GGATTTCCTTGAATTTGATG+GGG + chr2.4:45273393-45273412 None:intergenic 35.0%
GGGATTTCCTTGAATTTGAT+GGG + chr2.4:45273394-45273413 None:intergenic 35.0%
TAATGACCTTGGAATGGAAA+AGG + chr2.4:45276500-45276519 None:intergenic 35.0%
TAGTGTTGTCGATCATCAAT+TGG - chr2.4:45274080-45274099 MS.gene85293:intron 35.0%
TATGCCATGTTTGTGTATAC+TGG - chr2.4:45272979-45272998 MS.gene85293:CDS 35.0%
TCGACAACACTACAACATAT+AGG + chr2.4:45274072-45274091 None:intergenic 35.0%
TGATAATATAGCAAGGTCTG+GGG - chr2.4:45275387-45275406 MS.gene85293:intron 35.0%
TGTTCATCACAACCTATGAA+GGG - chr2.4:45276198-45276217 MS.gene85293:CDS 35.0%
TTAATGCATTGAAAAAGGGG+AGG - chr2.4:45275211-45275230 MS.gene85293:CDS 35.0%
TTTGGAAACACCTCAAATGA+TGG + chr2.4:45276527-45276546 None:intergenic 35.0%
! CAATGAGAGAAAACTTGCTT+TGG - chr2.4:45276556-45276575 MS.gene85293:intron 35.0%
! CTTAAGAGGTATGCTAAATC+TGG - chr2.4:45273688-45273707 MS.gene85293:intron 35.0%
! GCGGACAAACTATTAATTTG+AGG - chr2.4:45274995-45275014 MS.gene85293:intron 35.0%
! GCTATAACATTTGAGTTTGG+TGG + chr2.4:45276374-45276393 None:intergenic 35.0%
! TCTTACTAGGTAATTGGCTT+TGG + chr2.4:45276685-45276704 None:intergenic 35.0%
! TTCCAAAGCACTTCAAACTT+AGG - chr2.4:45275238-45275257 MS.gene85293:CDS 35.0%
! TTTGAGTTTGGTGGAGATAA+TGG + chr2.4:45276365-45276384 None:intergenic 35.0%
!! AAATAATCACAACTTGCTGC+TGG - chr2.4:45273787-45273806 MS.gene85293:intron 35.0%
!! AATTGCTGGAAGTTGATGAA+AGG - chr2.4:45272871-45272890 MS.gene85293:CDS 35.0%
!! GATGATGTTAGGTTTGCTAT+TGG + chr2.4:45276257-45276276 None:intergenic 35.0%
!! TATTGAGTGCTGATGATGTT+AGG + chr2.4:45276268-45276287 None:intergenic 35.0%
!! TGTAGATTTGTTGATGAGGT+AGG + chr2.4:45276299-45276318 None:intergenic 35.0%
!!! ACTTTCACCTCCATCTTTTT+TGG + chr2.4:45275430-45275449 None:intergenic 35.0%
!!! CATCTTTTTTGGTTGTGTTC+GGG + chr2.4:45275419-45275438 None:intergenic 35.0%
!!! CCATCTTTTTTGGTTGTGTT+CGG + chr2.4:45275420-45275439 None:intergenic 35.0%
!!! GTTGTGATTATTTTGAGGCA+AGG + chr2.4:45273780-45273799 None:intergenic 35.0%
AAAATCTGATCGTGCCCTTA+TGG + chr2.4:45274479-45274498 None:intergenic 40.0%
AATAGAGGACCAATCATCTG+AGG - chr2.4:45274429-45274448 MS.gene85293:intron 40.0%
AGAATCTCCATTTGTGAACG+AGG + chr2.4:45276648-45276667 None:intergenic 40.0%
AGTGTAATTGTAGGGCATGT+TGG + chr2.4:45276413-45276432 None:intergenic 40.0%
AGTTTACCTGCTTACGAACT+GGG + chr2.4:45274718-45274737 None:intergenic 40.0%
ATATGGACAAAAGACAGCCA+AGG - chr2.4:45274643-45274662 MS.gene85293:intron 40.0%
ATGATTATGGCTCCCTTCAT+AGG + chr2.4:45276213-45276232 None:intergenic 40.0%
CAACTTCCCCATCAAATTCA+AGG - chr2.4:45273384-45273403 MS.gene85293:intron 40.0%
CCGAACACAACCAAAAAAGA+TGG - chr2.4:45275417-45275436 MS.gene85293:intron 40.0%
CCTCTCTATTGAGAATGATG+CGG - chr2.4:45274976-45274995 MS.gene85293:intron 40.0%
GAAGCTTGATGAAGCAAAGA+AGG - chr2.4:45273655-45273674 MS.gene85293:intron 40.0%
GAAGGGAAATACACAAGATG+CGG - chr2.4:45274252-45274271 MS.gene85293:intron 40.0%
GGGGATTTCCTTGAATTTGA+TGG + chr2.4:45273395-45273414 None:intergenic 40.0%
GGGTGTCAATGGAGAAAATA+TGG - chr2.4:45274626-45274645 MS.gene85293:intron 40.0%
GTGTTCATCACAACCTATGA+AGG - chr2.4:45276197-45276216 MS.gene85293:CDS 40.0%
TAGTTTACCTGCTTACGAAC+TGG + chr2.4:45274719-45274738 None:intergenic 40.0%
TATGATGAAAATCGTGGGTG+TGG - chr2.4:45273216-45273235 MS.gene85293:CDS 40.0%
TATGGACAAAAGACAGCCAA+GGG - chr2.4:45274644-45274663 MS.gene85293:intron 40.0%
TCATCAAAGAACTGACGAGT+AGG + chr2.4:45274292-45274311 None:intergenic 40.0%
TCATTACCCTCCATCATTTG+AGG - chr2.4:45276514-45276533 MS.gene85293:intron 40.0%
TGTATGAATCGAGATCATCG+AGG + chr2.4:45273037-45273056 None:intergenic 40.0%
TTGCTACAATCTGAAGGTGA+AGG + chr2.4:45274316-45274335 None:intergenic 40.0%
! AGCCTAAGTTTGAAGTGCTT+TGG + chr2.4:45275243-45275262 None:intergenic 40.0%
! GAAACACCTCAAATGATGGA+GGG + chr2.4:45276523-45276542 None:intergenic 40.0%
! GAGATAATGGTGAAGAGAAG+AGG + chr2.4:45276352-45276371 None:intergenic 40.0%
!! CTGGTGGAAGTTGATGATTA+TGG + chr2.4:45276226-45276245 None:intergenic 40.0%
!! GATTTGTTGATGAGGTAGGA+AGG + chr2.4:45276295-45276314 None:intergenic 40.0%
!! TAAGGAAGATGATGATGGGA+AGG - chr2.4:45275748-45275767 MS.gene85293:intron 40.0%
!!! TTTGCTATTGGTTTTGCTGC+TGG + chr2.4:45276245-45276264 None:intergenic 40.0%
AACTGACGAGTAGGTAACAC+TGG + chr2.4:45274283-45274302 None:intergenic 45.0%
AGCCTACTATCGTTGCAGTA+TGG - chr2.4:45274682-45274701 MS.gene85293:intron 45.0%
ATTGTAGGGCATGTTGGAGA+TGG + chr2.4:45276407-45276426 None:intergenic 45.0%
CCGCATCATTCTCAATAGAG+AGG + chr2.4:45274979-45274998 None:intergenic 45.0%
CCTACTATCGTTGCAGTATG+GGG - chr2.4:45274684-45274703 MS.gene85293:intron 45.0%
GCCTACTATCGTTGCAGTAT+GGG - chr2.4:45274683-45274702 MS.gene85293:intron 45.0%
GGAAACACCTCAAATGATGG+AGG + chr2.4:45276524-45276543 None:intergenic 45.0%
TCCATTTGTGAACGAGGACA+TGG + chr2.4:45276642-45276661 None:intergenic 45.0%
! AGGACCAATCATCTGAGGTT+GGG - chr2.4:45274434-45274453 MS.gene85293:intron 45.0%
! ATGATGGAGGGTAATGACCT+TGG + chr2.4:45276511-45276530 None:intergenic 45.0%
! GAGAGCTAGAGTGTCCATAA+GGG - chr2.4:45274462-45274481 MS.gene85293:intron 45.0%
! TTGCTCCCTTTTCCATTCCA+AGG - chr2.4:45276491-45276510 MS.gene85293:intron 45.0%
!! ACAGGTGCAAAGGTGTTTCA+AGG - chr2.4:45276166-45276185 MS.gene85293:CDS 45.0%
!! AGATGGTTGATGGGTGTCAA+TGG - chr2.4:45274615-45274634 MS.gene85293:intron 45.0%
CCCCATACTGCAACGATAGT+AGG + chr2.4:45274687-45274706 None:intergenic 50.0%
GATAGTAGGCTCGTGGACAA+GGG + chr2.4:45274673-45274692 None:intergenic 50.0%
GATGGGAAAATCATGCAGCC+AGG - chr2.4:45273321-45273340 MS.gene85293:CDS 50.0%
GCCATGTCCTCGTTCACAAA+TGG - chr2.4:45276638-45276657 MS.gene85293:CDS 50.0%
GGAGGGTAATGACCTTGGAA+TGG + chr2.4:45276506-45276525 None:intergenic 50.0%
TGCACCCAACCTCAGATGAT+TGG + chr2.4:45274441-45274460 None:intergenic 50.0%
TTCATGCCCAGTTCGTAAGC+AGG - chr2.4:45274709-45274728 MS.gene85293:intron 50.0%
! AATCATCTGAGGTTGGGTGC+AGG - chr2.4:45274440-45274459 MS.gene85293:intron 50.0%
! GAGGACCAATCATCTGAGGT+TGG - chr2.4:45274433-45274452 MS.gene85293:intron 50.0%
! GGAGAGCTAGAGTGTCCATA+AGG - chr2.4:45274461-45274480 MS.gene85293:intron 50.0%
!!! GCTATTGGTTTTGCTGCTGG+TGG + chr2.4:45276242-45276261 None:intergenic 50.0%
CGATAGTAGGCTCGTGGACA+AGG + chr2.4:45274674-45274693 None:intergenic 55.0%
CTGCAACGATAGTAGGCTCG+TGG + chr2.4:45274680-45274699 None:intergenic 55.0%
GGAAAGGTGGGAAGCGAACT+AGG - chr2.4:45275519-45275538 MS.gene85293:intron 55.0%
TCGTGGACAAGGGTTGCCCT+TGG + chr2.4:45274663-45274682 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 45272846 45276715 45272846 ID=MS.gene85293
chr2.4 mRNA 45272846 45276715 45272846 ID=MS.gene85293.t1;Parent=MS.gene85293
chr2.4 exon 45276596 45276715 45276596 ID=MS.gene85293.t1.exon1;Parent=MS.gene85293.t1
chr2.4 CDS 45276596 45276715 45276596 ID=cds.MS.gene85293.t1;Parent=MS.gene85293.t1
chr2.4 exon 45276139 45276222 45276139 ID=MS.gene85293.t1.exon2;Parent=MS.gene85293.t1
chr2.4 CDS 45276139 45276222 45276139 ID=cds.MS.gene85293.t1;Parent=MS.gene85293.t1
chr2.4 exon 45275753 45275923 45275753 ID=MS.gene85293.t1.exon3;Parent=MS.gene85293.t1
chr2.4 CDS 45275753 45275923 45275753 ID=cds.MS.gene85293.t1;Parent=MS.gene85293.t1
chr2.4 exon 45275057 45275308 45275057 ID=MS.gene85293.t1.exon4;Parent=MS.gene85293.t1
chr2.4 CDS 45275057 45275308 45275057 ID=cds.MS.gene85293.t1;Parent=MS.gene85293.t1
chr2.4 exon 45274831 45274944 45274831 ID=MS.gene85293.t1.exon5;Parent=MS.gene85293.t1
chr2.4 CDS 45274831 45274944 45274831 ID=cds.MS.gene85293.t1;Parent=MS.gene85293.t1
chr2.4 exon 45272846 45273391 45272846 ID=MS.gene85293.t1.exon6;Parent=MS.gene85293.t1
chr2.4 CDS 45272846 45273391 45272846 ID=cds.MS.gene85293.t1;Parent=MS.gene85293.t1
Gene Sequence

>MS.gene85293

ATGGAAGAAAAAGTTCAAACAATGAAATTGCTGGAAGTTGATGAAAGGCCACCATATGATAATAACAATAAAGATCTACAAAACAAGATCGATGAATCCTCTTCCTTCCATAAGAATACTGTAAGTTTTCATTTATGCCATGTTTGTGTATACTGGATTAATTTCCATATTTGATATTTGATTATTAGCCTCGATGATCTCGATTCATACATAAAATTTACAATTTTGTTCATTGCTTTATTTTCTAATGCTTTATAAAGAGTCATATACTTTGTTTAGGCAAATAGACTTGAGCATCTAATGCTTTATAAGTTGAAGTAGTTTTTTTTCTTTTTAAAGAAGTTGAAGTAGTTTTTGTTCTTTGTTAGCTTATGATGAAAATCGTGGGTGTGGACAAAGATTTCATCTCATCATTCTTTTATCTCAATTACTTATTTTGTTAACAAAAAAACCTAACCTAATAACTTGACATTTTGATGGGAAAATCATGCAGCCAGGATCTACTCTTTTATTAAGTTTGAGCCTTAATAACACAACACAACTTCCCCATCAAATTCAAGGAAATCCCCAAATCCAAGTAAGTGATTAATCTTATTAGTGAATTTAAAGCATGTTTTTTTTAATTAACTTTCACACATAAATTTTAATTTATTTATATGAGAGTATATAAAGTAATTAATCTTATTAGTGAATTAAGAGCATGTGTTATTTTTAATTAACTTTCACACATAAATTTAAATTTATTTATATGATAGTATAAAAGTAATTTTTTGATTTGATAATTGCTCTAAGATTGGCAATATACGAGTGAAGCTTGATGAAGCAAAGAAGGAGAATGAAAACTTAAGAGGTATGCTAAATCTGGTCAACGATCGATGCAATGTTCTTCAGAATCGTTTACTTTTGGCTATGCATATGCATCAATCATCTTCCTTGCCTCAAAATAATCACAACTTGCTGCTGGTAAGTTTGACTTCAAAATTTTGTTCACATATCCTTATTTGATTCACACTCATTTTTTTGTTCTATTAAATTTGCTTGAAATATTTAATGATCTCATGTATTTGGTCAACACGTCTTAATATTTTCAATACTTATAGCTACAATTAATTAATTATATCCCGTCTACTTACAAATAGATAAACACAAAAAATAAACAAATAATTAATGATAATAAAAAATTTGAAACGTATAATTTTTTGTTGTTTATGTAATTAACATGGCCTATATGTTGTAGTGTTGTCGATCATCAATTGGTTAGACACATTCAATATGATAAATTAAACTCTAATTTTGGATTTGGTGAATGTGGTCAATTAAACTTTTTCAACCTATGATATTTTCAACTTGTAATAAATATAATTGCATATATTAACCTTTGTACATTTTTTCTTCATAATTAATTAGAAGGGAAATACACAAGATGCGGAGAAACCAGTGTTACCTACTCGTCAGTTCTTTGATGAGCCTTCACCTTCAGATTGTAGCAAAAATAATGGATTTGCCATAGTGGAAAACAATGAAAACAACATGGGAAGAAATTTTGCATGTGAGTACATTAATGAAGGTGAAATAAATAGCAAAATAGAGGACCAATCATCTGAGGTTGGGTGCAGGAGAGCTAGAGTGTCCATAAGGGCACGATCAGATTTTGCATTTGTAAGGACATGCATGCAACTCTTCTTTTTTTAATTAGTACAATAATCTAATAATATAGTAATCAATAATGATTAATTTGTATTTTAAGTGTTAATTAATTTGTAATGTTTAGATGGTTGATGGGTGTCAATGGAGAAAATATGGACAAAAGACAGCCAAGGGCAACCCTTGTCCACGAGCCTACTATCGTTGCAGTATGGGGACTTCATGCCCAGTTCGTAAGCAGGTAAACTATTATTTAATTTCATAGATCGTACCATTTTGATATGATATAACCTAAAAACTATCATAAGTTCGAGTTTTTTTAAATCTTATAAATCGATTTTATAGAAGTGAGTTGGCTTTTATGCAAAATATAAGAATTTCAATTGGAAATCGTTAAATGTTAGCCCAACCTAAATTCTAAAAAATGTTATTTCAAAAATTGAACAATTTTTGTTTTTTATGTTTGTGGTATCACTTCACTTGTTTCCTCTCTATTGAGAATGATGCGGACAAACTATTAATTTGAGGGGAAAATTAAAGAAAAAAATACAATGTTGTCAATATATTTTTGTTTTGCAATTTTTTTTGTCTTGTTTTTATATGTATAATAGAAATGGAGAAAGTACAATATTTTATATCTTGATATTTGAGCAAATATGTTACTTTGTTCTGATGTTCGTCCTTAATTGATGATGCACTACAATGAGACATCACAACAATTTTAATGCATTGAAAAAGGGGAGGAACATTCCAAAGCACTTCAAACTTAGGCTCCAATTACATTACAAAAACTTTTTCAGTTTTTATCTTTTAAAATTTTATGTTCATTTTAAATTTCTATCAAAGATATGGAAGACTATTAGTTCTTACTAGATGGAGGTGAAAGAATAGTTCAATTGATAATATAGCAAGGTCTGGGGTTCGAACCCGAACACAACCAAAAAAGATGGAGGTGAAAGTTTCGTATTAATAACATGATAACCGAACAATAAATAAAATGGAAGACTCATGTAATGAATGTTTGTGAAAGGAAAGGTGGGAAGCGAACTAGGAAGAACACATTTTCAAAGTTAATGAAAAAAGATGTATTTAGCATTTTCAAATATTTCGAAAATGGGGTTCATCTATTTTCAAATATTATAAGGGTATTGTTTCTCATATTAGGGTCTTTTTAAACGAGTGTTCTAAAGACACTCTTTATGGATTCTCATTTAATAAACTATCTATTTAAAGTTCTTTATGCCACTAATTAGCATTATAAGGAAGATGATGATGGGAAGGAAACAATTATTTATTTGAGCTTTGATACCTACACATTCAACCTAAAAGAATGAAATGAGAATTTCTATAGCTCATATTCTTGCAGTAATTGATTATCCTCCAATAGTTGGTTCTTGAATTTTGCAACACTCTGAATAAACATTATTTTAACAAATTTTTCACTTATTTGTAGTCTCAAATATGTACTTCTGTTCAAAACCATGAAAACTTGTCTCATTTACTAATTGTAAGTGCACTTTTCATAAAAAGTCATGAATACATCATAAGGAATAATGATGAGACTATTTTAAGGATCAAATTATTGTTGTGTAATTCTGTTTTAAAATGGTGTCTATATGAATGAAATTTTTTGCATATTGTTAACTCAATATATGATATTAATTACATTGCAATTAACAGGTGCAAAGGTGTTTCAAGGATGAGAGTGTGTTCATCACAACCTATGAAGGGAGCCATAATCATCAACTTCCACCAGCAGCAAAACCAATAGCAAACCTAACATCATCAGCACTCAATACATTCCTTCCTACCTCATCAACAAATCTACAACAATATGGCAACAATCTAACAAATACATTCCTCTTCTCTTCACCATTATCTCCACCAAACTCAAATGTTATAGCTACTTTCTCTCCATCTCCAACATGCCCTACAATTACACTTGATTTTACACTCCCTCCAAGTAACTATTTACAATTCAAAAATCATAAACAATCATCATTGCTCCCTTTTCCATTCCAAGGTCATTACCCTCCATCATTTGAGGTGTTTCCAAATATGATAAACAATGAGAGAAAACTTGCTTTGGTTGATGTTGTGAGTGAAGCTCTAGAAAAAGATCCTTCTCTCAAGGAAGCATTATTTTCAGCCATGTCCTCGTTCACAAATGGAGATTCTTCAAATATTAATAACCAAAGCCAATTACCTAGTAAGAGTAGTGGTTAA
Protein sequence

>MS.gene85293.t1

MEEKVQTMKLLEVDERPPYDNNNKDLQNKIDESSSFHKNTPGSTLLLSLSLNNTTQLPHQIQGNPQIQIGNIRVKLDEAKKENENLRGMLNLVNDRCNVLQNRLLLAMHMHQSSSLPQNNHNLLLKGNTQDAEKPVLPTRQFFDEPSPSDCSKNNGFAIVENNENNMGRNFACEYINEGEINSKIEDQSSEVGCRRARVSIRARSDFAFMVDGCQWRKYGQKTAKGNPCPRAYYRCSMGTSCPVRKQVQRCFKDESVFITTYEGSHNHQLPPAAKPIANLTSSALNTFLPTSSTNLQQYGNNLTNTFLFSSPLSPPNSNVIATFSPSPTCPTITLDFTLPPSNYLQFKNHKQSSLLPFPFQGHYPPSFEVFPNMINNERKLALVDVVSEALEKDPSLKEALFSAMSSFTNGDSSNINNQSQLPSKSSG