AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86246

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86246.t1 MTR_5g032420 99.379 322 2 0 1 322 1 322 0.0 651
MS.gene86246.t1 MTR_8g068890 81.366 322 53 4 1 322 1 315 3.01e-178 495
MS.gene86246.t1 MTR_3g100570 75.535 327 61 7 1 322 1 313 7.61e-170 474
MS.gene86246.t1 MTR_8g086070 38.006 321 159 3 3 322 18 299 8.98e-69 217
MS.gene86246.t1 MTR_7g066690 36.190 315 170 3 6 320 17 300 1.15e-65 209
MS.gene86246.t1 MTR_4g018750 35.331 317 174 3 6 322 15 300 1.07e-63 204
MS.gene86246.t1 MTR_1g090330 32.362 309 177 3 11 318 19 296 3.68e-54 179
MS.gene86246.t1 MTR_3g462140 32.704 318 182 3 4 320 13 299 1.33e-51 172
MS.gene86246.t1 MTR_4g018750 33.571 280 155 3 6 285 15 263 6.62e-47 160
MS.gene86246.t1 MTR_4g018750 28.249 177 114 4 150 317 31 203 2.34e-14 72.8
MS.gene86246.t1 MTR_8g086070 38.356 219 96 2 3 220 18 198 3.26e-45 153
MS.gene86246.t1 MTR_1g088995 30.671 313 178 6 6 318 33 306 3.31e-41 145
MS.gene86246.t1 MTR_1g088995 29.258 229 124 5 6 234 33 223 4.21e-21 90.5
MS.gene86246.t1 MTR_4g057850 25.552 317 188 9 7 315 23 299 1.50e-19 87.8
MS.gene86246.t1 MTR_4g057850 26.087 184 129 2 127 309 15 192 8.09e-12 65.5
MS.gene86246.t1 MTR_4g012850 22.956 318 200 7 6 319 16 292 1.73e-16 79.0
MS.gene86246.t1 MTR_3g104500 23.922 255 178 7 73 320 77 322 2.02e-16 79.0
MS.gene86246.t1 MTR_5g009500 24.047 341 198 9 3 319 35 338 6.52e-16 77.8
MS.gene86246.t1 MTR_5g009500 25.510 196 137 4 131 319 31 224 4.82e-11 63.5
MS.gene86246.t1 MTR_5g055410 26.316 304 183 7 22 319 37 305 2.63e-15 76.3
MS.gene86246.t1 MTR_7g117030 25.382 327 215 12 12 321 23 337 3.12e-15 75.9
MS.gene86246.t1 MTR_7g117030 26.549 226 127 7 134 322 14 237 1.16e-14 73.9
MS.gene86246.t1 MTR_2g102010 23.529 323 201 7 4 321 15 296 7.88e-15 73.9
MS.gene86246.t1 MTR_8g027975 24.561 342 197 11 3 319 48 353 3.03e-14 73.2
MS.gene86246.t1 MTR_8g027975 26.633 199 131 4 131 319 44 237 2.79e-12 67.0
MS.gene86246.t1 MTR_1g087840 25.249 301 190 10 22 319 35 303 7.44e-14 71.2
MS.gene86246.t1 MTR_5g073040 25.538 325 182 9 11 322 40 317 1.81e-13 70.5
MS.gene86246.t1 MTR_4g119770 21.765 340 216 9 5 322 17 328 1.94e-13 70.5
MS.gene86246.t1 MTR_1g097890 27.586 232 150 5 90 319 89 304 2.68e-13 69.7
MS.gene86246.t1 MTR_7g064980 21.277 329 217 7 3 322 22 317 3.20e-13 69.7
MS.gene86246.t1 MTR_6g034515 25.246 305 171 10 21 316 82 338 3.58e-13 69.7
MS.gene86246.t1 MTR_5g030530 24.611 321 194 9 9 319 14 296 1.02e-12 67.8
MS.gene86246.t1 MTR_5g030530 24.390 205 127 5 132 319 6 199 3.03e-11 63.5
MS.gene86246.t1 MTR_4g088545 26.804 194 127 5 131 316 9 195 1.83e-12 66.6
MS.gene86246.t1 MTR_2g098070 25.214 234 160 7 67 293 102 327 1.85e-12 67.8
MS.gene86246.t1 MTR_4g088545 26.804 194 127 5 131 316 9 195 3.39e-12 66.2
MS.gene86246.t1 MTR_4g088545 26.702 191 131 4 131 316 9 195 4.06e-12 66.2
MS.gene86246.t1 MTR_4g088545 23.939 330 202 11 2 319 8 300 7.06e-11 62.4
MS.gene86246.t1 MTR_7g109135 23.511 319 201 8 8 319 24 306 7.97e-12 65.5
MS.gene86246.t1 MTR_7g068340 27.586 174 115 4 150 321 103 267 1.25e-11 65.5
MS.gene86246.t1 MTR_8g076760 26.908 249 162 9 85 320 65 306 2.73e-11 63.9
MS.gene86246.t1 MTR_6g091800 24.038 208 128 6 128 317 12 207 3.17e-11 63.5
MS.gene86246.t1 MTR_3g028590 23.355 304 189 9 6 300 97 365 3.17e-11 64.3
MS.gene86246.t1 MTR_3g028590 23.355 304 189 9 6 300 93 361 3.28e-11 63.9
MS.gene86246.t1 MTR_8g066975 25.776 322 189 10 9 319 14 296 3.32e-11 63.5
MS.gene86246.t1 MTR_3g098460 27.128 188 126 5 138 322 205 384 4.13e-11 63.9
MS.gene86246.t1 MTR_8g096050 24.468 188 131 4 138 322 227 406 4.66e-11 63.9
MS.gene86246.t1 MTR_2g436930 22.090 335 213 9 9 321 18 326 4.78e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86246.t1 AT4G24570 74.224 322 74 4 1 322 1 313 2.38e-168 470
MS.gene86246.t1 AT2G22500 75.232 323 67 6 1 322 1 311 4.84e-164 459
MS.gene86246.t1 AT5G09470 59.824 341 110 8 1 321 1 334 5.46e-123 356
MS.gene86246.t1 AT5G19760 38.272 324 150 4 3 320 15 294 5.89e-70 220
MS.gene86246.t1 AT3G54110 36.677 319 171 4 4 322 13 300 5.27e-67 213
MS.gene86246.t1 AT5G58970 36.308 325 158 5 6 319 12 298 8.37e-66 209
MS.gene86246.t1 AT5G58970 26.222 225 115 7 9 228 127 305 1.27e-12 67.8
MS.gene86246.t1 AT1G14140 33.119 311 173 5 11 318 21 299 1.59e-52 175
MS.gene86246.t1 AT4G03115 31.761 318 171 6 3 318 35 308 2.29e-47 162
MS.gene86246.t1 AT4G03115 31.761 318 171 6 3 318 67 340 4.08e-47 162
MS.gene86246.t1 AT5G58970 35.385 260 126 4 6 254 12 240 4.53e-45 155
MS.gene86246.t1 AT5G58970 26.590 173 122 3 150 317 32 204 6.98e-17 79.7
MS.gene86246.t1 AT4G03115 33.077 260 155 5 61 318 3 245 6.94e-43 148
MS.gene86246.t1 AT4G03115 33.471 242 145 4 79 318 10 237 6.87e-41 143
MS.gene86246.t1 AT4G03115 28.879 232 124 4 3 234 35 225 4.05e-25 101
MS.gene86246.t1 AT4G03115 28.879 232 124 4 3 234 35 225 4.22e-25 101
MS.gene86246.t1 AT5G01340 26.562 320 192 8 7 320 18 300 5.21e-22 94.7
MS.gene86246.t1 AT5G01340 26.178 191 134 2 120 309 3 187 2.59e-12 67.0
MS.gene86246.t1 AT5G46800 26.235 324 188 9 9 319 14 299 1.62e-17 82.0
MS.gene86246.t1 AT5G46800 22.727 198 144 2 131 319 5 202 7.51e-13 68.6
MS.gene86246.t1 AT5G46800 26.235 324 188 9 9 319 14 299 1.62e-17 82.0
MS.gene86246.t1 AT5G46800 22.727 198 144 2 131 319 5 202 7.51e-13 68.6
MS.gene86246.t1 AT5G46800 26.235 324 188 9 9 319 14 299 1.62e-17 82.0
MS.gene86246.t1 AT5G46800 22.727 198 144 2 131 319 5 202 7.51e-13 68.6
MS.gene86246.t1 AT5G56450 25.969 258 169 7 73 320 81 326 4.65e-17 80.9
MS.gene86246.t1 AT1G25380 25.413 303 190 7 22 320 37 307 7.49e-15 74.7
MS.gene86246.t1 AT1G25380 25.413 303 190 7 22 320 37 307 7.49e-15 74.7
MS.gene86246.t1 AT1G07030 26.687 326 188 9 6 322 36 319 1.81e-14 73.6
MS.gene86246.t1 AT4G01100 24.419 344 193 10 4 319 40 344 5.49e-14 72.4
MS.gene86246.t1 AT4G01100 26.396 197 134 3 131 319 35 228 2.83e-12 67.0
MS.gene86246.t1 AT2G33820 25.225 333 192 13 5 319 13 306 6.96e-14 71.6
MS.gene86246.t1 AT4G01100 25.843 267 164 7 80 319 35 294 2.65e-13 69.7
MS.gene86246.t1 AT3G53940 23.423 333 197 9 3 319 70 360 3.16e-13 70.1
MS.gene86246.t1 AT3G51870 30.286 175 109 5 150 321 107 271 5.14e-13 69.7
MS.gene86246.t1 AT3G51870 22.848 302 185 9 20 319 105 360 4.10e-12 67.0
MS.gene86246.t1 AT4G27940 23.647 351 213 12 12 320 71 408 5.78e-13 69.7
MS.gene86246.t1 AT4G26180 26.471 204 130 6 127 317 13 209 5.81e-13 68.6
MS.gene86246.t1 AT4G01100 24.058 345 209 13 3 319 39 358 8.97e-13 68.6
MS.gene86246.t1 AT4G26180 26.471 204 130 6 127 317 13 209 9.58e-13 68.6
MS.gene86246.t1 AT3G53940 24.870 193 128 4 138 318 73 260 1.21e-12 67.8
MS.gene86246.t1 AT5G01500 27.528 178 118 4 146 321 131 299 3.06e-12 67.4
MS.gene86246.t1 AT5G01500 24.000 300 184 7 20 319 133 388 1.92e-11 65.1
MS.gene86246.t1 AT5G17400 22.712 295 188 7 6 293 14 275 3.52e-12 66.6
MS.gene86246.t1 AT3G55640 23.590 195 134 3 138 321 38 228 4.44e-12 65.5
MS.gene86246.t1 AT3G55640 23.590 195 134 3 138 321 38 228 4.44e-12 65.5
MS.gene86246.t1 AT5G66380 28.718 195 121 8 136 322 12 196 7.00e-12 65.5
MS.gene86246.t1 AT2G39970 23.656 279 178 8 71 317 44 319 1.36e-11 65.1
MS.gene86246.t1 AT4G28390 22.517 302 194 7 6 300 83 351 1.63e-11 65.1
MS.gene86246.t1 AT4G28390 22.517 302 194 7 6 300 83 351 1.63e-11 65.1
MS.gene86246.t1 AT3G21390 22.659 331 202 9 9 313 22 324 1.67e-11 64.7
MS.gene86246.t1 AT2G26360 23.053 321 183 11 3 317 112 374 2.08e-11 64.7
MS.gene86246.t1 AT3G21390 22.659 331 202 9 9 313 44 346 2.31e-11 64.3
MS.gene86246.t1 AT5G51050 28.571 189 124 5 138 322 223 404 2.40e-11 64.7
MS.gene86246.t1 AT1G79900 23.293 249 172 5 75 319 50 283 5.26e-11 62.8
MS.gene86246.t1 AT2G33820 25.604 207 140 7 116 316 3 201 6.82e-11 62.4
MS.gene86246.t1 AT3G55640 20.679 324 217 7 6 322 38 328 8.61e-11 62.4
MS.gene86246.t1 AT3G55640 20.679 324 217 7 6 322 38 328 8.61e-11 62.4

Find 116 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCCGACCGGCACTTGCTTT+CGG 0.332288 5.1:+22972353 MS.gene86246:CDS
GGACTTATTGCCGGCGGGGT+TGG 0.357971 5.1:+22972626 MS.gene86246:CDS
TGTTGTTCTGTTTGTTACTT+TGG 0.360137 5.1:+22973132 MS.gene86246:CDS
ACCACCTTATGCTGGTGCTT+TGG 0.365642 5.1:+22973021 MS.gene86246:CDS
CGAACCATGGCAACGTCGGC+GGG 0.400237 5.1:-22972665 None:intergenic
CGGAGGATCTGAACCGGGTT+CGG 0.406065 5.1:-22972335 None:intergenic
ATCGAGGCTGGACTTATTGC+CGG 0.411260 5.1:+22972617 MS.gene86246:CDS
CCCACACGAGGCTGAGGAAC+CGG 0.415972 5.1:-22972413 None:intergenic
AACATGGATCGTGGTTGTTC+CGG 0.428474 5.1:-22972381 None:intergenic
CTGCTTCGCCATTCTAGTAA+TGG 0.443816 5.1:-22972750 None:intergenic
GCCGGTCGGAGGATCTGAAC+CGG 0.451478 5.1:-22972341 None:intergenic
GGTGTAACAGCTTTGTTCTC+CGG 0.452972 5.1:+22972473 MS.gene86246:CDS
GCATCTCCGCCACTGTGCTC+AGG 0.455298 5.1:+22972495 MS.gene86246:CDS
CTGTTTCGCAAGATCGAGGC+TGG 0.456258 5.1:+22972605 MS.gene86246:CDS
CATTACTAGAATGGCGAAGC+AGG 0.462623 5.1:+22972751 MS.gene86246:CDS
GAGCACAGTGGCGGAGATGC+CGG 0.463595 5.1:-22972492 None:intergenic
ATCTGAACCGGGTTCGGCTT+AGG 0.465925 5.1:-22972329 None:intergenic
GGTCCCTGCCTTGAAATTGT+AGG 0.469679 5.1:-22973103 None:intergenic
GATTTATTCCTACAATTTCA+AGG 0.470932 5.1:+22973095 MS.gene86246:CDS
GGCGGCGGTTGCGTCGAATC+CGG 0.471512 5.1:+22972946 MS.gene86246:CDS
GCTGGTCACAGCTTCACAGC+TGG 0.472881 5.1:+22972826 MS.gene86246:CDS
ATAGAGTCCCATCCTGGTGG+TGG 0.474302 5.1:-22972531 None:intergenic
TATTCCTACAATTTCAAGGC+AGG 0.475430 5.1:+22973099 MS.gene86246:CDS
GGGTCCTATGGCTCTTTACA+AGG 0.475795 5.1:+22973072 MS.gene86246:CDS
GGCGGGGTTGGTGCTGCGAT+CGG 0.475997 5.1:+22972638 MS.gene86246:CDS
GCACTTGCTTTCGGTCAAAC+CGG 0.477758 5.1:+22972362 MS.gene86246:CDS
TCCGGTTCCTCAGCCTCGTG+TGG 0.482332 5.1:+22972412 MS.gene86246:CDS
TGTGGAACATCCTGCGATGA+TGG 0.487564 5.1:-22972255 None:intergenic
GTGTGACCAGTCTGTGGCGC+GGG 0.488791 5.1:+22972777 MS.gene86246:CDS
AAGGTTCGCATGCAACTTCA+AGG 0.489176 5.1:+22972296 MS.gene86246:CDS
AGTTCGTGCTGAGGGTCCTA+TGG 0.492843 5.1:+22973060 MS.gene86246:CDS
GCAAGTTCGCAAGTTGTTGA+AGG 0.493746 5.1:+22973156 MS.gene86246:CDS
CAAGGAGATGATTCTTGAGA+AGG 0.493818 5.1:+22972865 MS.gene86246:CDS
GGTGTTATGCGTGATGGACT+TGG 0.494172 5.1:+22972887 MS.gene86246:CDS
AGCCGATGGGAGACTCCCAC+CGG 0.495248 5.1:+22972694 MS.gene86246:CDS
CAATCCAAAGCACCAGCATA+AGG 0.495820 5.1:-22973025 None:intergenic
CAGCAAAACTAGCTGTCACA+TGG 0.510059 5.1:-22972913 None:intergenic
GAACAACCACGATCCATGTT+GGG 0.513576 5.1:+22972384 MS.gene86246:CDS
TAGTTTCTCTGCTGAGCCGG+TGG 0.518095 5.1:-22972710 None:intergenic
AGGCTGGACTTATTGCCGGC+GGG 0.524366 5.1:+22972621 MS.gene86246:CDS
GACTGTGAACCGCGCCATGC+TGG 0.524934 5.1:+22972808 MS.gene86246:CDS
TTGAAGACAGTTCGTGCTGA+GGG 0.525849 5.1:+22973052 MS.gene86246:CDS
TGATGACCCGCGCCACAGAC+TGG 0.533752 5.1:-22972783 None:intergenic
GGCTTCATATGATCAATTCA+AGG 0.534751 5.1:+22972847 MS.gene86246:CDS
CATGAAGGTTGAAGCTGGAA+AGG 0.536275 5.1:+22972997 MS.gene86246:CDS
TCGAACCATGGCAACGTCGG+CGG 0.536548 5.1:-22972666 None:intergenic
GGAAAGGAACCACCTTATGC+TGG 0.537944 5.1:+22973013 MS.gene86246:CDS
TTATAGTTTCTCTGCTGAGC+CGG 0.539922 5.1:-22972713 None:intergenic
CACCAGGATGGGACTCTATG+AGG 0.546356 5.1:+22972535 MS.gene86246:CDS
TAACCCCGCCGACGTTGCCA+TGG 0.547137 5.1:+22972661 MS.gene86246:CDS
CGGTGGATGTTATTAAGACA+AGG 0.547567 5.1:+22972966 MS.gene86246:CDS
AATCCCTTGTAAAGAGCCAT+AGG 0.547600 5.1:-22973076 None:intergenic
GTCCGTCTTGTTCAACAAGA+AGG 0.557738 5.1:+22972452 MS.gene86246:CDS
CGTGTGGGCCTTATCTCAGT+CGG 0.561147 5.1:+22972428 MS.gene86246:CDS
CACCTTCTTGTTGAACAAGA+CGG 0.561342 5.1:-22972454 None:intergenic
TGTCGACGCCATTACTAGAA+TGG 0.561691 5.1:+22972742 MS.gene86246:CDS
AACCTCATAGAGTCCCATCC+TGG 0.563410 5.1:-22972537 None:intergenic
ATGGTTCGAATGCAAGCCGA+TGG 0.563779 5.1:+22972680 MS.gene86246:CDS
AAGTGGACAGATCGTGAAGC+CGG 0.563971 5.1:+22972569 MS.gene86246:CDS
AAACAGCGGCATAGTGCCAC+CGG 0.564991 5.1:-22972588 None:intergenic
ATGAACATGAAGGTTGAAGC+TGG 0.565752 5.1:+22972992 MS.gene86246:CDS
AAGGAGATGATTCTTGAGAA+GGG 0.566148 5.1:+22972866 MS.gene86246:CDS
GACGGACTCCGACTGAGATA+AGG 0.567027 5.1:-22972436 None:intergenic
CTGTACTCCACCACCAGGAT+GGG 0.568929 5.1:+22972524 MS.gene86246:CDS
TGCTGCGGGATTTGTGGCGG+CGG 0.569156 5.1:+22972931 MS.gene86246:CDS
CTTGTCTTAATAACATCCAC+CGG 0.572366 5.1:-22972965 None:intergenic
ATCGGCTTGCATTCGAACCA+TGG 0.573789 5.1:-22972678 None:intergenic
AGCCGGTGGGAGTCTCCCAT+CGG 0.573898 5.1:-22972696 None:intergenic
GGAACAACCACGATCCATGT+TGG 0.574391 5.1:+22972383 MS.gene86246:CDS
AGTTTCTCTGCTGAGCCGGT+GGG 0.574910 5.1:-22972709 None:intergenic
CCGGTTCCTCAGCCTCGTGT+GGG 0.577991 5.1:+22972413 MS.gene86246:CDS
TTTGAAGACAGTTCGTGCTG+AGG 0.579339 5.1:+22973051 MS.gene86246:CDS
ACAAACAGAACAACAGTGAA+AGG 0.581480 5.1:-22973124 None:intergenic
CGAACCTTGATAAGATCTAG+TGG 0.582015 5.1:-22972281 None:intergenic
ATAAGGCCCACACGAGGCTG+AGG 0.584839 5.1:-22972419 None:intergenic
ATTCCTACAATTTCAAGGCA+GGG 0.587080 5.1:+22973100 MS.gene86246:CDS
GAACCGGAGTAGACCCAACA+TGG 0.587129 5.1:-22972397 None:intergenic
GGCTGGACTTATTGCCGGCG+GGG 0.587219 5.1:+22972622 MS.gene86246:CDS
CGAAAGCAAGTGCCGGTCGG+AGG 0.591618 5.1:-22972352 None:intergenic
GGTGTGACCAGTCTGTGGCG+CGG 0.596360 5.1:+22972776 MS.gene86246:CDS
GATCGCAGCACCAACCCCGC+CGG 0.597142 5.1:-22972636 None:intergenic
GGTATTGCTTCCATCATCGC+AGG 0.597300 5.1:+22972245 MS.gene86246:CDS
ACTGTACTCCACCACCAGGA+TGG 0.597836 5.1:+22972523 MS.gene86246:CDS
CCGGTCGGAGGATCTGAACC+GGG 0.601331 5.1:-22972340 None:intergenic
CCCGGTTCAGATCCTCCGAC+CGG 0.604021 5.1:+22972340 MS.gene86246:CDS
GCCTCGATCTTGCGAAACAG+CGG 0.604552 5.1:-22972602 None:intergenic
GATCCATGTTGGGTCTACTC+CGG 0.609921 5.1:+22972394 MS.gene86246:CDS
GTGTTATGCGTGATGGACTT+GGG 0.610332 5.1:+22972888 MS.gene86246:CDS
ACATCCACTAGATCTTATCA+AGG 0.613126 5.1:+22972277 MS.gene86246:CDS
GGCGGTTGCGTCGAATCCGG+TGG 0.613152 5.1:+22972949 MS.gene86246:CDS
GCCGCTGTTTCGCAAGATCG+AGG 0.616216 5.1:+22972601 MS.gene86246:CDS
GTTTGACCGAAAGCAAGTGC+CGG 0.620216 5.1:-22972359 None:intergenic
GACCGAAAGCAAGTGCCGGT+CGG 0.622497 5.1:-22972355 None:intergenic
AGGAAGGTGTGACCAGTCTG+TGG 0.623057 5.1:+22972771 MS.gene86246:CDS
AAACGCGCCTAAGCCGAACC+CGG 0.624848 5.1:+22972322 MS.gene86246:CDS
CATTCGAACCATGGCAACGT+CGG 0.625579 5.1:-22972669 None:intergenic
GGTCCTATGGCTCTTTACAA+GGG 0.627240 5.1:+22973073 MS.gene86246:CDS
ACTAGAATGGCGAAGCAGGA+AGG 0.629489 5.1:+22972755 MS.gene86246:CDS
TGGTTCGAATGCAAGCCGAT+GGG 0.641550 5.1:+22972681 MS.gene86246:CDS
GATAAGATCTAGTGGATGTG+TGG 0.642726 5.1:-22972273 None:intergenic
GAGAAGGGTGTTATGCGTGA+TGG 0.643473 5.1:+22972881 MS.gene86246:CDS
GACAAGGGTGATGAACATGA+AGG 0.643767 5.1:+22972982 MS.gene86246:CDS
TGTCTGCCTGAGCACAGTGG+CGG 0.644365 5.1:-22972501 None:intergenic
CTGTGAAGCTGTGACCAGCA+TGG 0.646036 5.1:-22972822 None:intergenic
AGACACTGTACTCCACCACC+AGG 0.648345 5.1:+22972519 MS.gene86246:CDS
GAGGCTGGACTTATTGCCGG+CGG 0.651692 5.1:+22972620 MS.gene86246:CDS
CTCATAGAGTCCCATCCTGG+TGG 0.654507 5.1:-22972534 None:intergenic
AGTAGACCCAACATGGATCG+TGG 0.654896 5.1:-22972390 None:intergenic
TCCAAAGCACCAGCATAAGG+TGG 0.656451 5.1:-22973022 None:intergenic
GAACCATGGCAACGTCGGCG+GGG 0.658264 5.1:-22972664 None:intergenic
AGCAAAACTAGCTGTCACAT+GGG 0.664704 5.1:-22972912 None:intergenic
TGGACAGATCGTGAAGCCGG+TGG 0.673996 5.1:+22972572 MS.gene86246:CDS
AAGCTGTGACCAGCATGGCG+CGG 0.685288 5.1:-22972817 None:intergenic
GGTGGATGTTATTAAGACAA+GGG 0.685805 5.1:+22972967 MS.gene86246:CDS
ACTGAGATAAGGCCCACACG+AGG 0.739468 5.1:-22972425 None:intergenic
CAGTGTCTGCCTGAGCACAG+TGG 0.746160 5.1:-22972504 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GATTTATTCCTACAATTTCA+AGG + chr5.1:22973095-22973114 MS.gene86246:CDS 25.0%
! TGTTGTTCTGTTTGTTACTT+TGG + chr5.1:22973132-22973151 MS.gene86246:CDS 30.0%
AAGGAGATGATTCTTGAGAA+GGG + chr5.1:22972866-22972885 MS.gene86246:CDS 35.0%
ACAAACAGAACAACAGTGAA+AGG - chr5.1:22973127-22973146 None:intergenic 35.0%
ACATCCACTAGATCTTATCA+AGG + chr5.1:22972277-22972296 MS.gene86246:CDS 35.0%
ATGAGGTTCTCAAAAACAAG+TGG + chr5.1:22972552-22972571 MS.gene86246:CDS 35.0%
ATTCCTACAATTTCAAGGCA+GGG + chr5.1:22973100-22973119 MS.gene86246:CDS 35.0%
CTTGTCTTAATAACATCCAC+CGG - chr5.1:22972968-22972987 None:intergenic 35.0%
GGCTTCATATGATCAATTCA+AGG + chr5.1:22972847-22972866 MS.gene86246:CDS 35.0%
GGTGGATGTTATTAAGACAA+GGG + chr5.1:22972967-22972986 MS.gene86246:CDS 35.0%
TATTCCTACAATTTCAAGGC+AGG + chr5.1:22973099-22973118 MS.gene86246:CDS 35.0%
AATCCCTTGTAAAGAGCCAT+AGG - chr5.1:22973079-22973098 None:intergenic 40.0%
AGCAAAACTAGCTGTCACAT+GGG - chr5.1:22972915-22972934 None:intergenic 40.0%
ATGAACATGAAGGTTGAAGC+TGG + chr5.1:22972992-22973011 MS.gene86246:CDS 40.0%
CAAGGAGATGATTCTTGAGA+AGG + chr5.1:22972865-22972884 MS.gene86246:CDS 40.0%
CACCTTCTTGTTGAACAAGA+CGG - chr5.1:22972457-22972476 None:intergenic 40.0%
CGAACCTTGATAAGATCTAG+TGG - chr5.1:22972284-22972303 None:intergenic 40.0%
CGGTGGATGTTATTAAGACA+AGG + chr5.1:22972966-22972985 MS.gene86246:CDS 40.0%
TTATAGTTTCTCTGCTGAGC+CGG - chr5.1:22972716-22972735 None:intergenic 40.0%
! GATAAGATCTAGTGGATGTG+TGG - chr5.1:22972276-22972295 None:intergenic 40.0%
!!! GGTGTCAAAGGTTTTGTTGA+AGG + chr5.1:22972221-22972240 MS.gene86246:CDS 40.0%
!!! GTCAAAGGTTTTGTTGAAGG+CGG + chr5.1:22972224-22972243 MS.gene86246:CDS 40.0%
AACATGGATCGTGGTTGTTC+CGG - chr5.1:22972384-22972403 None:intergenic 45.0%
AAGGTTCGCATGCAACTTCA+AGG + chr5.1:22972296-22972315 MS.gene86246:CDS 45.0%
CAGCAAAACTAGCTGTCACA+TGG - chr5.1:22972916-22972935 None:intergenic 45.0%
CATGAAGGTTGAAGCTGGAA+AGG + chr5.1:22972997-22973016 MS.gene86246:CDS 45.0%
CATTACTAGAATGGCGAAGC+AGG + chr5.1:22972751-22972770 MS.gene86246:CDS 45.0%
CTGCTTCGCCATTCTAGTAA+TGG - chr5.1:22972753-22972772 None:intergenic 45.0%
GAACAACCACGATCCATGTT+GGG + chr5.1:22972384-22972403 MS.gene86246:CDS 45.0%
GACAAGGGTGATGAACATGA+AGG + chr5.1:22972982-22973001 MS.gene86246:CDS 45.0%
GCAAGTTCGCAAGTTGTTGA+AGG + chr5.1:22973156-22973175 MS.gene86246:CDS 45.0%
GGTCCTATGGCTCTTTACAA+GGG + chr5.1:22973073-22973092 MS.gene86246:CDS 45.0%
TGTCGACGCCATTACTAGAA+TGG + chr5.1:22972742-22972761 MS.gene86246:CDS 45.0%
TTGAAGACAGTTCGTGCTGA+GGG + chr5.1:22973052-22973071 MS.gene86246:CDS 45.0%
TTTGAAGACAGTTCGTGCTG+AGG + chr5.1:22973051-22973070 MS.gene86246:CDS 45.0%
! CAATCCAAAGCACCAGCATA+AGG - chr5.1:22973028-22973047 None:intergenic 45.0%
! GTCCGTCTTGTTCAACAAGA+AGG + chr5.1:22972452-22972471 MS.gene86246:CDS 45.0%
!! GGTGTAACAGCTTTGTTCTC+CGG + chr5.1:22972473-22972492 MS.gene86246:CDS 45.0%
!! GTGTTATGCGTGATGGACTT+GGG + chr5.1:22972888-22972907 MS.gene86246:CDS 45.0%
!! TAGTTTTGCTGCGGGATTTG+TGG + chr5.1:22972925-22972944 MS.gene86246:CDS 45.0%
!!! TGTGACAGCTAGTTTTGCTG+CGG + chr5.1:22972916-22972935 MS.gene86246:CDS 45.0%
AACCTCATAGAGTCCCATCC+TGG - chr5.1:22972540-22972559 None:intergenic 50.0%
AAGTGGACAGATCGTGAAGC+CGG + chr5.1:22972569-22972588 MS.gene86246:CDS 50.0%
ACCACCTTATGCTGGTGCTT+TGG + chr5.1:22973021-22973040 MS.gene86246:CDS 50.0%
ACTAGAATGGCGAAGCAGGA+AGG + chr5.1:22972755-22972774 MS.gene86246:CDS 50.0%
AGTAGACCCAACATGGATCG+TGG - chr5.1:22972393-22972412 None:intergenic 50.0%
ATCGAGGCTGGACTTATTGC+CGG + chr5.1:22972617-22972636 MS.gene86246:CDS 50.0%
ATGGTTCGAATGCAAGCCGA+TGG + chr5.1:22972680-22972699 MS.gene86246:CDS 50.0%
CATTCGAACCATGGCAACGT+CGG - chr5.1:22972672-22972691 None:intergenic 50.0%
GATCCATGTTGGGTCTACTC+CGG + chr5.1:22972394-22972413 MS.gene86246:CDS 50.0%
GCACTTGCTTTCGGTCAAAC+CGG + chr5.1:22972362-22972381 MS.gene86246:CDS 50.0%
GGAAAGGAACCACCTTATGC+TGG + chr5.1:22973013-22973032 MS.gene86246:CDS 50.0%
GGAACAACCACGATCCATGT+TGG + chr5.1:22972383-22972402 MS.gene86246:CDS 50.0%
GGGTCCTATGGCTCTTTACA+AGG + chr5.1:22973072-22973091 MS.gene86246:CDS 50.0%
GGTATTGCTTCCATCATCGC+AGG + chr5.1:22972245-22972264 MS.gene86246:CDS 50.0%
GGTCCCTGCCTTGAAATTGT+AGG - chr5.1:22973106-22973125 None:intergenic 50.0%
GTTTGACCGAAAGCAAGTGC+CGG - chr5.1:22972362-22972381 None:intergenic 50.0%
TGGTTCGAATGCAAGCCGAT+GGG + chr5.1:22972681-22972700 MS.gene86246:CDS 50.0%
TGTGGAACATCCTGCGATGA+TGG - chr5.1:22972258-22972277 None:intergenic 50.0%
! TCCAAAGCACCAGCATAAGG+TGG - chr5.1:22973025-22973044 None:intergenic 50.0%
! TTTTGCTGCGGGATTTGTGG+CGG + chr5.1:22972928-22972947 MS.gene86246:CDS 50.0%
!! ATCGGCTTGCATTCGAACCA+TGG - chr5.1:22972681-22972700 None:intergenic 50.0%
!! GAGAAGGGTGTTATGCGTGA+TGG + chr5.1:22972881-22972900 MS.gene86246:CDS 50.0%
!! GGTGTTATGCGTGATGGACT+TGG + chr5.1:22972887-22972906 MS.gene86246:CDS 50.0%
!!! GTGACAGCTAGTTTTGCTGC+GGG + chr5.1:22972917-22972936 MS.gene86246:CDS 50.0%
AAACAGCGGCATAGTGCCAC+CGG - chr5.1:22972591-22972610 None:intergenic 55.0%
ACTGAGATAAGGCCCACACG+AGG - chr5.1:22972428-22972447 None:intergenic 55.0%
ACTGTACTCCACCACCAGGA+TGG + chr5.1:22972523-22972542 MS.gene86246:CDS 55.0%
AGACACTGTACTCCACCACC+AGG + chr5.1:22972519-22972538 MS.gene86246:CDS 55.0%
AGTTCGTGCTGAGGGTCCTA+TGG + chr5.1:22973060-22973079 MS.gene86246:CDS 55.0%
AGTTTCTCTGCTGAGCCGGT+GGG - chr5.1:22972712-22972731 None:intergenic 55.0%
ATAGAGTCCCATCCTGGTGG+TGG - chr5.1:22972534-22972553 None:intergenic 55.0%
ATCTGAACCGGGTTCGGCTT+AGG - chr5.1:22972332-22972351 None:intergenic 55.0%
CACCAGGATGGGACTCTATG+AGG + chr5.1:22972535-22972554 MS.gene86246:CDS 55.0%
CTCATAGAGTCCCATCCTGG+TGG - chr5.1:22972537-22972556 None:intergenic 55.0%
CTGTACTCCACCACCAGGAT+GGG + chr5.1:22972524-22972543 MS.gene86246:CDS 55.0%
CTGTGAAGCTGTGACCAGCA+TGG - chr5.1:22972825-22972844 None:intergenic 55.0%
CTGTTTCGCAAGATCGAGGC+TGG + chr5.1:22972605-22972624 MS.gene86246:CDS 55.0%
GAACCGGAGTAGACCCAACA+TGG - chr5.1:22972400-22972419 None:intergenic 55.0%
GCCTCGATCTTGCGAAACAG+CGG - chr5.1:22972605-22972624 None:intergenic 55.0%
TAGTTTCTCTGCTGAGCCGG+TGG - chr5.1:22972713-22972732 None:intergenic 55.0%
! AGGAAGGTGTGACCAGTCTG+TGG + chr5.1:22972771-22972790 MS.gene86246:CDS 55.0%
! CGTGTGGGCCTTATCTCAGT+CGG + chr5.1:22972428-22972447 MS.gene86246:CDS 55.0%
! GACGGACTCCGACTGAGATA+AGG - chr5.1:22972439-22972458 None:intergenic 55.0%
AAACGCGCCTAAGCCGAACC+CGG + chr5.1:22972322-22972341 MS.gene86246:CDS 60.0%
AAGCTGTGACCAGCATGGCG+CGG - chr5.1:22972820-22972839 None:intergenic 60.0%
AGGCTGGACTTATTGCCGGC+GGG + chr5.1:22972621-22972640 MS.gene86246:CDS 60.0%
ATAAGGCCCACACGAGGCTG+AGG - chr5.1:22972422-22972441 None:intergenic 60.0%
CAGTGTCTGCCTGAGCACAG+TGG - chr5.1:22972507-22972526 None:intergenic 60.0%
CGGAGGATCTGAACCGGGTT+CGG - chr5.1:22972338-22972357 None:intergenic 60.0%
CTCCGACCGGCACTTGCTTT+CGG + chr5.1:22972353-22972372 MS.gene86246:CDS 60.0%
GAGGCTGGACTTATTGCCGG+CGG + chr5.1:22972620-22972639 MS.gene86246:CDS 60.0%
GCTGGTCACAGCTTCACAGC+TGG + chr5.1:22972826-22972845 MS.gene86246:CDS 60.0%
TCGAACCATGGCAACGTCGG+CGG - chr5.1:22972669-22972688 None:intergenic 60.0%
TGGACAGATCGTGAAGCCGG+TGG + chr5.1:22972572-22972591 MS.gene86246:CDS 60.0%
TGTCTGCCTGAGCACAGTGG+CGG - chr5.1:22972504-22972523 None:intergenic 60.0%
! GCCGCTGTTTCGCAAGATCG+AGG + chr5.1:22972601-22972620 MS.gene86246:CDS 60.0%
!! GACCGAAAGCAAGTGCCGGT+CGG - chr5.1:22972358-22972377 None:intergenic 60.0%
AGCCGATGGGAGACTCCCAC+CGG + chr5.1:22972694-22972713 MS.gene86246:CDS 65.0%
AGCCGGTGGGAGTCTCCCAT+CGG - chr5.1:22972699-22972718 None:intergenic 65.0%
CCCACACGAGGCTGAGGAAC+CGG - chr5.1:22972416-22972435 None:intergenic 65.0%
CCCGGTTCAGATCCTCCGAC+CGG + chr5.1:22972340-22972359 MS.gene86246:CDS 65.0%
CCGGTCGGAGGATCTGAACC+GGG - chr5.1:22972343-22972362 None:intergenic 65.0%
CCGGTTCCTCAGCCTCGTGT+GGG + chr5.1:22972413-22972432 MS.gene86246:CDS 65.0%
CGAACCATGGCAACGTCGGC+GGG - chr5.1:22972668-22972687 None:intergenic 65.0%
GAACCATGGCAACGTCGGCG+GGG - chr5.1:22972667-22972686 None:intergenic 65.0%
GACTGTGAACCGCGCCATGC+TGG + chr5.1:22972808-22972827 MS.gene86246:CDS 65.0%
GCATCTCCGCCACTGTGCTC+AGG + chr5.1:22972495-22972514 MS.gene86246:CDS 65.0%
GCCGGTCGGAGGATCTGAAC+CGG - chr5.1:22972344-22972363 None:intergenic 65.0%
GGACTTATTGCCGGCGGGGT+TGG + chr5.1:22972626-22972645 MS.gene86246:CDS 65.0%
GGCTGGACTTATTGCCGGCG+GGG + chr5.1:22972622-22972641 MS.gene86246:CDS 65.0%
GGTGTGACCAGTCTGTGGCG+CGG + chr5.1:22972776-22972795 MS.gene86246:CDS 65.0%
GTGTGACCAGTCTGTGGCGC+GGG + chr5.1:22972777-22972796 MS.gene86246:CDS 65.0%
TAACCCCGCCGACGTTGCCA+TGG + chr5.1:22972661-22972680 MS.gene86246:CDS 65.0%
TCCGGTTCCTCAGCCTCGTG+TGG + chr5.1:22972412-22972431 MS.gene86246:CDS 65.0%
TGATGACCCGCGCCACAGAC+TGG - chr5.1:22972786-22972805 None:intergenic 65.0%
TGCTGCGGGATTTGTGGCGG+CGG + chr5.1:22972931-22972950 MS.gene86246:CDS 65.0%
! GAGCACAGTGGCGGAGATGC+CGG - chr5.1:22972495-22972514 None:intergenic 65.0%
!! CGAAAGCAAGTGCCGGTCGG+AGG - chr5.1:22972355-22972374 None:intergenic 65.0%
GATCGCAGCACCAACCCCGC+CGG - chr5.1:22972639-22972658 None:intergenic 70.0%
GGCGGCGGTTGCGTCGAATC+CGG + chr5.1:22972946-22972965 MS.gene86246:CDS 70.0%
GGCGGTTGCGTCGAATCCGG+TGG + chr5.1:22972949-22972968 MS.gene86246:CDS 70.0%
!! GGCGGGGTTGGTGCTGCGAT+CGG + chr5.1:22972638-22972657 MS.gene86246:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 22972218 22973186 22972218 ID=MS.gene86246
chr5.1 mRNA 22972218 22973186 22972218 ID=MS.gene86246.t1;Parent=MS.gene86246
chr5.1 exon 22972218 22973186 22972218 ID=MS.gene86246.t1.exon1;Parent=MS.gene86246.t1
chr5.1 CDS 22972218 22973186 22972218 ID=cds.MS.gene86246.t1;Parent=MS.gene86246.t1
Gene Sequence

>MS.gene86246

ATGGGTGTCAAAGGTTTTGTTGAAGGCGGTATTGCTTCCATCATCGCAGGATGTTCCACACATCCACTAGATCTTATCAAGGTTCGCATGCAACTTCAAGGCGAAAACGCGCCTAAGCCGAACCCGGTTCAGATCCTCCGACCGGCACTTGCTTTCGGTCAAACCGGAACAACCACGATCCATGTTGGGTCTACTCCGGTTCCTCAGCCTCGTGTGGGCCTTATCTCAGTCGGAGTCCGTCTTGTTCAACAAGAAGGTGTAACAGCTTTGTTCTCCGGCATCTCCGCCACTGTGCTCAGGCAGACACTGTACTCCACCACCAGGATGGGACTCTATGAGGTTCTCAAAAACAAGTGGACAGATCGTGAAGCCGGTGGCACTATGCCGCTGTTTCGCAAGATCGAGGCTGGACTTATTGCCGGCGGGGTTGGTGCTGCGATCGGTAACCCCGCCGACGTTGCCATGGTTCGAATGCAAGCCGATGGGAGACTCCCACCGGCTCAGCAGAGAAACTATAAATCTGTTGTCGACGCCATTACTAGAATGGCGAAGCAGGAAGGTGTGACCAGTCTGTGGCGCGGGTCATCATTGACTGTGAACCGCGCCATGCTGGTCACAGCTTCACAGCTGGCTTCATATGATCAATTCAAGGAGATGATTCTTGAGAAGGGTGTTATGCGTGATGGACTTGGGACCCATGTGACAGCTAGTTTTGCTGCGGGATTTGTGGCGGCGGTTGCGTCGAATCCGGTGGATGTTATTAAGACAAGGGTGATGAACATGAAGGTTGAAGCTGGAAAGGAACCACCTTATGCTGGTGCTTTGGATTGTGCTTTGAAGACAGTTCGTGCTGAGGGTCCTATGGCTCTTTACAAGGGATTTATTCCTACAATTTCAAGGCAGGGACCTTTCACTGTTGTTCTGTTTGTTACTTTGGAGCAAGTTCGCAAGTTGTTGAAGGATTTCTGA
Protein sequence

>MS.gene86246.t1

MGVKGFVEGGIASIIAGCSTHPLDLIKVRMQLQGENAPKPNPVQILRPALAFGQTGTTTIHVGSTPVPQPRVGLISVGVRLVQQEGVTALFSGISATVLRQTLYSTTRMGLYEVLKNKWTDREAGGTMPLFRKIEAGLIAGGVGAAIGNPADVAMVRMQADGRLPPAQQRNYKSVVDAITRMAKQEGVTSLWRGSSLTVNRAMLVTASQLASYDQFKEMILEKGVMRDGLGTHVTASFAAGFVAAVASNPVDVIKTRVMNMKVEAGKEPPYAGALDCALKTVRAEGPMALYKGFIPTISRQGPFTVVLFVTLEQVRKLLKDF