AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86368

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86368.t1 MTR_2g103370 99.161 834 7 0 1 834 1 834 0.0 1709
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86368.t1 AT2G41620 76.344 837 194 4 1 834 1 836 0.0 1315
MS.gene86368.t1 AT3G57350 73.628 838 214 6 1 834 1 835 0.0 1256

Find 214 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATTCTGGCAATGAGATTT+TGG 0.159071 2.3:+1569554 MS.gene86368:CDS
CGCTCAGTTCCCTCCTCTTC+AGG 0.194338 2.3:+1562353 MS.gene86368:CDS
CAGATTCGGTTGAGGGATTA+TGG 0.218780 2.3:+1566730 MS.gene86368:intron
AAAGAATGTCATTGATTATT+GGG 0.226836 2.3:+1566379 MS.gene86368:CDS
ATGATGGTTCAAATTTATTT+AGG 0.234703 2.3:-1568366 None:intergenic
AAAAGAATGTCATTGATTAT+TGG 0.235409 2.3:+1566378 MS.gene86368:CDS
CCCTGGTCAAATTGTTTCTA+TGG 0.240119 2.3:+1565385 MS.gene86368:CDS
AGTAGGGGACTTACTTTAAA+TGG 0.241555 2.3:-1565539 None:intergenic
AATAATCAATGACATTCTTT+TGG 0.246454 2.3:-1566376 None:intergenic
GGAATCATGTTGATGCATTA+AGG 0.269660 2.3:+1570279 MS.gene86368:CDS
CAAGGGTGCATACGAAAGTT+TGG 0.278688 2.3:+1566129 MS.gene86368:CDS
TGATTATATGATGAAAGTTT+TGG 0.290773 2.3:+1564430 MS.gene86368:CDS
TTCACATCAATTTGCTCCTT+TGG 0.293661 2.3:+1567943 MS.gene86368:CDS
CTTTCTGAAGGCATCGGTAC+TGG 0.302430 2.3:+1568550 MS.gene86368:CDS
CAGAAGTTTCCACAATTATC+AGG 0.308981 2.3:+1568602 MS.gene86368:CDS
TCCACAATTATCAGGCAATA+TGG 0.312023 2.3:+1568610 MS.gene86368:CDS
TAGACCCATCGCAGATAAAA+AGG 0.313748 2.3:+1565460 MS.gene86368:CDS
AATGCTTAAAAGCAAGATAT+AGG 0.316195 2.3:-1568425 None:intergenic
GGTTATCCTTCTGAGCTTCC+TGG 0.316241 2.3:-1564394 None:intergenic
AGCCACAAGCTTATTACTTC+TGG 0.317192 2.3:-1570593 None:intergenic
TCTACTCCACAAGTCTCTTC+TGG 0.319960 2.3:+1565410 MS.gene86368:CDS
ACTTCTGGCCTTATCCATTC+TGG 0.335329 2.3:+1569540 MS.gene86368:CDS
TAATAATTTCACCTGACATC+TGG 0.338858 2.3:-1569594 None:intergenic
GCAGCAGTAGCCACCTCTTC+TGG 0.339368 2.3:-1568076 None:intergenic
CTGCTGTACGCCATCATATC+AGG 0.341394 2.3:+1568172 MS.gene86368:CDS
CGCTTCAGCTCTTACAGTCT+TGG 0.344037 2.3:-1562626 None:intergenic
AAAGAATCCGCGCCTTCCTT+CGG 0.347541 2.3:+1566612 MS.gene86368:CDS
AAAGATATGGCACCTTGTTC+AGG 0.349423 2.3:+1566189 MS.gene86368:CDS
TCGACGCTTGTTGCCTCAAC+AGG 0.349996 2.3:-1563840 None:intergenic
GATGGTGAGAGCCGGACTTC+TGG 0.353288 2.3:+1569525 MS.gene86368:CDS
TGCCGGAGAAGAAGGAGAAT+TGG 0.353697 2.3:+1568837 MS.gene86368:CDS
TCTAACTGAATTATTGTTAA+GGG 0.357730 2.3:+1568780 MS.gene86368:CDS
GTTGAGGGATTATGGGGTTC+TGG 0.358855 2.3:+1566738 MS.gene86368:CDS
TTTAAGCATTCAATTGCTTC+CGG 0.360646 2.3:+1568438 MS.gene86368:CDS
GGCTGTATTATACTTGTCTA+AGG 0.380243 2.3:+1568459 MS.gene86368:CDS
TCGTTCTCAGGTTCATGAAA+TGG 0.382869 2.3:+1564355 MS.gene86368:intron
AAGAATCCGCGCCTTCCTTC+GGG 0.385464 2.3:+1566613 MS.gene86368:CDS
CAATGTTGTTTAGAGGAACT+TGG 0.385531 2.3:+1562570 MS.gene86368:intron
TTCTAACTGAATTATTGTTA+AGG 0.393622 2.3:+1568779 MS.gene86368:CDS
TTTGAAGTCATTTGAGTTGA+AGG 0.396716 2.3:+1563307 MS.gene86368:CDS
AAGGGTGCATACGAAAGTTT+GGG 0.396800 2.3:+1566130 MS.gene86368:CDS
AGATTCGGTTGAGGGATTAT+GGG 0.398506 2.3:+1566731 MS.gene86368:intron
TTCAGAGAGGCACTTGTTGA+TGG 0.398841 2.3:-1569472 None:intergenic
CAGTAACGATGCGAAAGATA+TGG 0.400785 2.3:+1566176 MS.gene86368:CDS
TATCTAATGTAGGCTTCTCT+TGG 0.401085 2.3:+1566569 MS.gene86368:intron
GCTGCTGCTGCAGTCGGCGG+TGG 0.402676 2.3:+1568688 MS.gene86368:CDS
GCATGCCAGTGTCAGGAGGC+TGG 0.405039 2.3:+1568910 MS.gene86368:CDS
GCTTCTCTTGGTGGTGGTGT+TGG 0.405273 2.3:+1566581 MS.gene86368:CDS
CTGAATAGAGCAAGGCAGTC+TGG 0.408430 2.3:+1565512 MS.gene86368:CDS
GAAGGTTGCTCTGACTTGTC+TGG 0.416937 2.3:+1570412 MS.gene86368:CDS
ATGCTTAAAAGCAAGATATA+GGG 0.417403 2.3:-1568424 None:intergenic
CTCACAGAGTGGATTAATAC+TGG 0.419658 2.3:+1568043 MS.gene86368:CDS
ATAGCTTGCCGCAACTTTCA+AGG 0.420161 2.3:+1566111 MS.gene86368:intron
TTTCTTGTGGTTCAAATTGT+CGG 0.427428 2.3:+1568258 MS.gene86368:CDS
AATTCACAAATTGTCAAGAC+TGG 0.427511 2.3:+1570256 MS.gene86368:CDS
GGGCAAGGCGCCACCTTGAG+TGG 0.430038 2.3:+1566400 MS.gene86368:CDS
TGCTGCGCAGATTCGGTTGA+GGG 0.430889 2.3:+1566723 MS.gene86368:intron
CTAATGTAGGCTTCTCTTGG+TGG 0.431600 2.3:+1566572 MS.gene86368:intron
GCAAGAGGTGCCGGAGAAGA+AGG 0.439378 2.3:+1568829 MS.gene86368:CDS
TTGTATACCAGACCTCTTGA+AGG 0.447490 2.3:+1570394 MS.gene86368:CDS
AACAGCAATATCAGGTCCTC+GGG 0.447540 2.3:-1570336 None:intergenic
AGCGGCTTGTTCGAGAAGCT+TGG 0.447832 2.3:-1562325 None:intergenic
AACACATCAACAGCAATATC+AGG 0.449707 2.3:-1570344 None:intergenic
TCCCAATTCTCCTTCTTCTC+CGG 0.453235 2.3:-1568839 None:intergenic
CATCGGTACTGGGCAAAAGT+TGG 0.453262 2.3:+1568561 MS.gene86368:CDS
GTGAAGTAGATCGGTCCAAC+TGG 0.454319 2.3:-1562295 None:intergenic
CATGCCAGTGTCAGGAGGCT+GGG 0.455198 2.3:+1568911 MS.gene86368:CDS
TGTTGATACCACATGGCAAC+AGG 0.460280 2.3:+1566795 MS.gene86368:CDS
GCCGGAGAAGAAGGAGAATT+GGG 0.461170 2.3:+1568838 MS.gene86368:CDS
TTACTTGGTGCAAGAGGTGC+CGG 0.461950 2.3:+1568820 MS.gene86368:CDS
GCGTCTTGGTGATCTTCAAA+TGG 0.466910 2.3:+1568645 MS.gene86368:CDS
TTAGCAATCATGTTTGTCCT+TGG 0.468677 2.3:-1565344 None:intergenic
GAATCATGTTGATGCATTAA+GGG 0.468904 2.3:+1570280 MS.gene86368:CDS
AGCAATGTGGGTGCCCGCCC+TGG 0.473304 2.3:+1565368 MS.gene86368:CDS
AAGCTCAGAAGGATAACCTT+AGG 0.474420 2.3:+1564399 MS.gene86368:CDS
CCAAAATCTCATTGCCAGAA+TGG 0.474737 2.3:-1569554 None:intergenic
TGCAAATCATCCAAACTGTA+TGG 0.475966 2.3:-1568337 None:intergenic
GTTCAAATTGTCGGCTGTGC+GGG 0.476027 2.3:+1568267 MS.gene86368:CDS
TGAAGCGGCATGCCAGTGTC+AGG 0.479438 2.3:+1568903 MS.gene86368:CDS
GATCGGCTAGCACAATTGAT+AGG 0.481113 2.3:-1568519 None:intergenic
TTGCTGCGCAGATTCGGTTG+AGG 0.481656 2.3:+1566722 MS.gene86368:intron
ATCGGTACTGGGCAAAAGTT+GGG 0.482123 2.3:+1568562 MS.gene86368:CDS
CAAGCTGCTGCTGCAGTCGG+CGG 0.483082 2.3:+1568685 MS.gene86368:CDS
TCGTCCATTGTTCTAAGTGA+TGG 0.486274 2.3:+1568307 MS.gene86368:CDS
AATGTTGAGAACTGGTGATC+GGG 0.486654 2.3:+1568117 MS.gene86368:CDS
TCTCATTGCCAGAATGGATA+AGG 0.486894 2.3:-1569548 None:intergenic
TGGTCTGATTCCATACAGTT+TGG 0.493704 2.3:+1568327 MS.gene86368:CDS
GGCAATGGTCTCTGCCGTCC+AGG 0.496524 2.3:+1564376 MS.gene86368:CDS
GAAAGTTTGGGCGTTGATGC+TGG 0.497812 2.3:+1566142 MS.gene86368:CDS
TCAATTGTGCTAGCCGATCA+TGG 0.498941 2.3:+1568523 MS.gene86368:CDS
TGTTGCCATGTGGTATCAAC+AGG 0.498947 2.3:-1566793 None:intergenic
AACAATGGACGAAGATCCAC+TGG 0.500001 2.3:-1568296 None:intergenic
GGTGGACAGTTGTCGTGGAC+TGG 0.502670 2.3:+1568706 MS.gene86368:CDS
AGAGCAACCTTCAAGAGGTC+TGG 0.503916 2.3:-1570401 None:intergenic
GGCAAGGCGCCACCTTGAGT+GGG 0.505759 2.3:+1566401 MS.gene86368:CDS
TGCGATGGGTCTACTAGTCA+TGG 0.505854 2.3:-1565450 None:intergenic
GAATAACACATTACTTGTGC+GGG 0.509007 2.3:-1566462 None:intergenic
AATGGATAAGGCCAGAAGTC+CGG 0.510606 2.3:-1569536 None:intergenic
TGTGAAAGAATGTTGAGAAC+TGG 0.510947 2.3:+1568109 MS.gene86368:CDS
TGGGCAAAAGTTGGGAGTGA+TGG 0.514092 2.3:+1568570 MS.gene86368:CDS
TTTCTGAAGGCATCGGTACT+GGG 0.514619 2.3:+1568551 MS.gene86368:CDS
AATAATTTCACCTGACATCT+GGG 0.517279 2.3:-1569593 None:intergenic
TCTAAGGAAGCAGGAGATGA+AGG 0.518714 2.3:+1568475 MS.gene86368:CDS
CAGTTGTCGTGGACTGGTAG+AGG 0.522609 2.3:+1568712 MS.gene86368:CDS
CAACAGCAATATCAGGTCCT+CGG 0.524800 2.3:-1570337 None:intergenic
GGCTAGCACAATTGATAGGT+GGG 0.527583 2.3:-1568515 None:intergenic
AGAAACAATTTGACCAGGGC+GGG 0.528867 2.3:-1565381 None:intergenic
GGATCTATGTATTTGCGTCT+TGG 0.529918 2.3:+1568631 MS.gene86368:CDS
ACTGAATTATTGTTAAGGGA+TGG 0.530872 2.3:+1568784 MS.gene86368:CDS
ATTTCCTTCAGAGTTTAAGC+AGG 0.532379 2.3:+1565309 MS.gene86368:CDS
TTATACTTGTCTAAGGAAGC+AGG 0.533407 2.3:+1568466 MS.gene86368:CDS
GGCTTGTTCGAGAAGCTTGG+AGG 0.534973 2.3:-1562322 None:intergenic
AAATCCTGCTTAAACTCTGA+AGG 0.537989 2.3:-1565313 None:intergenic
ATACTTACTACCCAGATGTC+AGG 0.538443 2.3:+1569583 MS.gene86368:CDS
ATAGAAATTCAGAAGAGAGT+TGG 0.538979 2.3:+1569426 MS.gene86368:CDS
ATTCACAAATTGTCAAGACT+GGG 0.541007 2.3:+1570257 MS.gene86368:CDS
TGCCGTCCAGGAAGCTCAGA+AGG 0.542467 2.3:+1564388 MS.gene86368:CDS
GGATAAATCTTCGTTCGCCA+TGG 0.543632 2.3:-1562274 None:intergenic
ACAAGTCTCTTCTGGTAGCA+TGG 0.544655 2.3:+1565418 MS.gene86368:CDS
AGGGAACTGAGCGGAAGGAG+CGG 0.547144 2.3:-1562343 None:intergenic
TCTTCGAGAACCTGATATGA+TGG 0.547163 2.3:-1568182 None:intergenic
TTCAGAAAGGACGCCATGAT+CGG 0.547176 2.3:-1568536 None:intergenic
TTGGGCGTTGATGCTGGTGG+TGG 0.547427 2.3:+1566148 MS.gene86368:CDS
GAATGTTGAGAACTGGTGAT+CGG 0.548567 2.3:+1568116 MS.gene86368:CDS
AGAGGTCTGGTATACAAGCT+TGG 0.551386 2.3:-1570388 None:intergenic
GAGTTGGTTGATCTCTAAGC+AGG 0.552770 2.3:-1568216 None:intergenic
GCACAAGCTGCTGCTGCAGT+CGG 0.553605 2.3:+1568682 MS.gene86368:CDS
TATAATCATTGAAACTCCTA+AGG 0.553720 2.3:-1564415 None:intergenic
ATGTAGGCTTCTCTTGGTGG+TGG 0.555114 2.3:+1566575 MS.gene86368:intron
GAAACAGATGCTGATGGATG+AGG 0.555847 2.3:+1566332 MS.gene86368:intron
CGTCGAAGAGTATCTGCAGC+AGG 0.556391 2.3:+1563857 MS.gene86368:CDS
TAAATGCAGAGCAGCTTGCA+AGG 0.557101 2.3:+1563282 MS.gene86368:CDS
AAGAATGTCATTGATTATTG+GGG 0.558574 2.3:+1566380 MS.gene86368:CDS
TAGCTTGCCGCAACTTTCAA+GGG 0.559302 2.3:+1566112 MS.gene86368:intron
TCCATATTGCCTGATAATTG+TGG 0.560589 2.3:-1568611 None:intergenic
GGTATCAAGCGCCACTGAGA+AGG 0.560618 2.3:-1569451 None:intergenic
GTGGATTAATACTGGAGGAA+TGG 0.561929 2.3:+1568051 MS.gene86368:CDS
GTATGCACCCTTGAAAGTTG+CGG 0.563942 2.3:-1566119 None:intergenic
AGAGTACCTAGTTGCAGCAA+TGG 0.566795 2.3:-1562659 None:intergenic
GAATTATTGTTAAGGGATGG+AGG 0.568335 2.3:+1568787 MS.gene86368:CDS
TGTCATTGATTATTGGGGCA+AGG 0.568514 2.3:+1566385 MS.gene86368:CDS
GCAAGATATAGGGATATACC+AGG 0.570594 2.3:-1568414 None:intergenic
CGGCTAGCACAATTGATAGG+TGG 0.571111 2.3:-1568516 None:intergenic
ACTTCGTGCTTTGAGAGCTA+AGG 0.571432 2.3:+1570460 MS.gene86368:CDS
TCTCACCATCTAACCTTCCA+CGG 0.572182 2.3:-1569512 None:intergenic
ATTTCTCATGTCCCCACTCA+AGG 0.575810 2.3:-1566413 None:intergenic
GAACTGGTGATCGGGTAGGC+CGG 0.576829 2.3:+1568125 MS.gene86368:CDS
TTAGACAAGTATAATACAGC+CGG 0.577630 2.3:-1568457 None:intergenic
GGTATACAAGCTTGGACATG+AGG 0.577743 2.3:-1570380 None:intergenic
TGGAGGAATGGTGCCAGAAG+AGG 0.578745 2.3:+1568063 MS.gene86368:CDS
GATTCGGTTGAGGGATTATG+GGG 0.578836 2.3:+1566732 MS.gene86368:intron
TCGGCGGTGGACAGTTGTCG+TGG 0.580039 2.3:+1568701 MS.gene86368:CDS
ATCCTTCTGAGCTTCCTGGA+CGG 0.581007 2.3:-1564390 None:intergenic
GGCGTCCTTTCTGAAGGCAT+CGG 0.581437 2.3:+1568544 MS.gene86368:CDS
GACAATGTGACAGACTCTGA+TGG 0.582532 2.3:+1570434 MS.gene86368:CDS
TGAATAACACATTACTTGTG+CGG 0.585399 2.3:-1566463 None:intergenic
ACATCCCAGCCTCCTGACAC+TGG 0.585632 2.3:-1568915 None:intergenic
ATACAGGCTTCTTGCTCGTG+AGG 0.588254 2.3:+1563256 MS.gene86368:intron
GATCATGGCGTCCTTTCTGA+AGG 0.591595 2.3:+1568538 MS.gene86368:CDS
CGAACGAAGATTTATCCAGT+TGG 0.592391 2.3:+1562280 MS.gene86368:CDS
GAAGGTTAGATGGTGAGAGC+CGG 0.594461 2.3:+1569517 MS.gene86368:CDS
CAACAAGTGCAACCTGAACA+AGG 0.594712 2.3:-1566201 None:intergenic
CACAGTCCATTGCTGCAACT+AGG 0.595342 2.3:+1562653 MS.gene86368:CDS
TACAGGCTTCTTGCTCGTGA+GGG 0.595779 2.3:+1563257 MS.gene86368:intron
ATGCTACCAGAAGAGACTTG+TGG 0.595882 2.3:-1565416 None:intergenic
GCAAGGCGCCACCTTGAGTG+GGG 0.595896 2.3:+1566402 MS.gene86368:CDS
CTAATTGAAACAGATGCTGA+TGG 0.595941 2.3:+1566326 MS.gene86368:intron
ATTGAGAGAACCTGAAGAGG+AGG 0.596014 2.3:-1562363 None:intergenic
CAGTACCGATGCCTTCAGAA+AGG 0.598482 2.3:-1568549 None:intergenic
ACCATAGAAACAATTTGACC+AGG 0.598681 2.3:-1565386 None:intergenic
GAGGTAATGTTGATCAACAA+AGG 0.600325 2.3:+1568731 MS.gene86368:CDS
GGGACATGAGAAATACATCA+TGG 0.600464 2.3:+1566422 MS.gene86368:CDS
AGGAATGGTGCCAGAAGAGG+TGG 0.600952 2.3:+1568066 MS.gene86368:CDS
AAGTCAGAGCAACCTTCAAG+AGG 0.601145 2.3:-1570406 None:intergenic
GCGATGGGTCTACTAGTCAT+GGG 0.603772 2.3:-1565449 None:intergenic
GTTGAGACGAACCCGAAGGA+AGG 0.608990 2.3:-1566624 None:intergenic
GGAGGAAGAGTGAAGTAGAT+CGG 0.609858 2.3:-1562304 None:intergenic
AGCGGCATGCCAGTGTCAGG+AGG 0.613053 2.3:+1568906 MS.gene86368:CDS
AGAGGAGGGAACTGAGCGGA+AGG 0.613962 2.3:-1562348 None:intergenic
TCAGGTTCATGAAATGGCAA+TGG 0.615202 2.3:+1564361 MS.gene86368:intron
CAGACCATCACTTAGAACAA+TGG 0.617186 2.3:-1568311 None:intergenic
TGCCATGTGGTATCAACAGG+TGG 0.627027 2.3:-1566790 None:intergenic
ATGATTGCTAATAGCAATGT+GGG 0.627930 2.3:+1565356 MS.gene86368:CDS
GCGATTGAGAGAACCTGAAG+AGG 0.631149 2.3:-1562366 None:intergenic
TTGAGAACTGGTGATCGGGT+AGG 0.632782 2.3:+1568121 MS.gene86368:CDS
TTGTAGCGGATCCAAATGCA+AGG 0.634980 2.3:+1568866 MS.gene86368:CDS
CAAGATATAGGGATATACCA+GGG 0.636735 2.3:-1568413 None:intergenic
TCAGGTCCTCGGGGATCTAG+AGG 0.636836 2.3:-1570326 None:intergenic
GCTGTGCGGGACTGCCCCAG+TGG 0.639313 2.3:+1568280 MS.gene86368:CDS
GCACGATAGAGGATTTCTTG+TGG 0.640475 2.3:+1568245 MS.gene86368:CDS
GCAGGATTTCAACATTACCA+AGG 0.642066 2.3:+1565327 MS.gene86368:CDS
TTGAGAGAACCTGAAGAGGA+GGG 0.643698 2.3:-1562362 None:intergenic
GGTTCAAATTGTCGGCTGTG+CGG 0.644450 2.3:+1568266 MS.gene86368:CDS
GTTGCAGCAATGGACTGTGA+CGG 0.646464 2.3:-1562649 None:intergenic
GACGAACCCGAAGGAAGGCG+CGG 0.646810 2.3:-1566619 None:intergenic
AAATGCAGAGCAGCTTGCAA+GGG 0.647635 2.3:+1563283 MS.gene86368:CDS
TCATGTTGATGCATTAAGGG+AGG 0.649430 2.3:+1570283 MS.gene86368:CDS
CATGATTGCTAATAGCAATG+TGG 0.654831 2.3:+1565355 MS.gene86368:CDS
CCATAGAAACAATTTGACCA+GGG 0.664122 2.3:-1565385 None:intergenic
GAGAGTTGGTTCCTTCTCAG+TGG 0.668521 2.3:+1569440 MS.gene86368:CDS
AGCCACCTGTTGATACCACA+TGG 0.668750 2.3:+1566788 MS.gene86368:CDS
GTTTCTTACAGCTCACAGAG+TGG 0.670484 2.3:+1568032 MS.gene86368:intron
TCTCATGTCCCCACTCAAGG+TGG 0.676167 2.3:-1566410 None:intergenic
TTCACAAATTGTCAAGACTG+GGG 0.679733 2.3:+1570258 MS.gene86368:CDS
AAGATATAGGGATATACCAG+GGG 0.683071 2.3:-1568412 None:intergenic
TAGAAACAATTTGACCAGGG+CGG 0.686037 2.3:-1565382 None:intergenic
ACAATGGACGAAGATCCACT+GGG 0.689644 2.3:-1568295 None:intergenic
ACAGAGTGGATTAATACTGG+AGG 0.690461 2.3:+1568046 MS.gene86368:CDS
AGTTTGGGCGTTGATGCTGG+TGG 0.704332 2.3:+1566145 MS.gene86368:CDS
GAGAACAAATAGCTTCAGAG+AGG 0.705513 2.3:-1569485 None:intergenic
CTGAAGAGGAGGGAACTGAG+CGG 0.706436 2.3:-1562352 None:intergenic
AGTCAAGACCTGTTGCCATG+TGG 0.718128 2.3:-1566803 None:intergenic
ACAGCAATATCAGGTCCTCG+GGG 0.757630 2.3:-1570335 None:intergenic
CAATGGACGAAGATCCACTG+GGG 0.814652 2.3:-1568294 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr2.3 gene 1562276 1570602 1562276 ID=MS.gene86368
chr2.3 mRNA 1562276 1570602 1562276 ID=MS.gene86368.t1;Parent=MS.gene86368
chr2.3 exon 1562276 1562374 1562276 ID=MS.gene86368.t1.exon1;Parent=MS.gene86368.t1
chr2.3 CDS 1562276 1562374 1562276 ID=cds.MS.gene86368.t1;Parent=MS.gene86368.t1
chr2.3 exon 1562583 1562674 1562583 ID=MS.gene86368.t1.exon2;Parent=MS.gene86368.t1
chr2.3 CDS 1562583 1562674 1562583 ID=cds.MS.gene86368.t1;Parent=MS.gene86368.t1
chr2.3 exon 1563262 1563328 1563262 ID=MS.gene86368.t1.exon3;Parent=MS.gene86368.t1
chr2.3 CDS 1563262 1563328 1563262 ID=cds.MS.gene86368.t1;Parent=MS.gene86368.t1
chr2.3 exon 1563819 1563878 1563819 ID=MS.gene86368.t1.exon4;Parent=MS.gene86368.t1
chr2.3 CDS 1563819 1563878 1563819 ID=cds.MS.gene86368.t1;Parent=MS.gene86368.t1
chr2.3 exon 1564365 1564454 1564365 ID=MS.gene86368.t1.exon5;Parent=MS.gene86368.t1
chr2.3 CDS 1564365 1564454 1564365 ID=cds.MS.gene86368.t1;Parent=MS.gene86368.t1
chr2.3 exon 1565284 1565547 1565284 ID=MS.gene86368.t1.exon6;Parent=MS.gene86368.t1
chr2.3 CDS 1565284 1565547 1565284 ID=cds.MS.gene86368.t1;Parent=MS.gene86368.t1
chr2.3 exon 1566115 1566210 1566115 ID=MS.gene86368.t1.exon7;Parent=MS.gene86368.t1
chr2.3 CDS 1566115 1566210 1566115 ID=cds.MS.gene86368.t1;Parent=MS.gene86368.t1
chr2.3 exon 1566339 1566470 1566339 ID=MS.gene86368.t1.exon8;Parent=MS.gene86368.t1
chr2.3 CDS 1566339 1566470 1566339 ID=cds.MS.gene86368.t1;Parent=MS.gene86368.t1
chr2.3 exon 1566581 1566634 1566581 ID=MS.gene86368.t1.exon9;Parent=MS.gene86368.t1
chr2.3 CDS 1566581 1566634 1566581 ID=cds.MS.gene86368.t1;Parent=MS.gene86368.t1
chr2.3 exon 1566733 1566816 1566733 ID=MS.gene86368.t1.exon10;Parent=MS.gene86368.t1
chr2.3 CDS 1566733 1566816 1566733 ID=cds.MS.gene86368.t1;Parent=MS.gene86368.t1
chr2.3 exon 1567878 1567964 1567878 ID=MS.gene86368.t1.exon11;Parent=MS.gene86368.t1
chr2.3 CDS 1567878 1567964 1567878 ID=cds.MS.gene86368.t1;Parent=MS.gene86368.t1
chr2.3 exon 1568043 1568945 1568043 ID=MS.gene86368.t1.exon12;Parent=MS.gene86368.t1
chr2.3 CDS 1568043 1568945 1568043 ID=cds.MS.gene86368.t1;Parent=MS.gene86368.t1
chr2.3 exon 1569423 1569604 1569423 ID=MS.gene86368.t1.exon13;Parent=MS.gene86368.t1
chr2.3 CDS 1569423 1569604 1569423 ID=cds.MS.gene86368.t1;Parent=MS.gene86368.t1
chr2.3 exon 1570190 1570481 1570190 ID=MS.gene86368.t1.exon14;Parent=MS.gene86368.t1
chr2.3 CDS 1570190 1570481 1570190 ID=cds.MS.gene86368.t1;Parent=MS.gene86368.t1
chr2.3 exon 1570579 1570602 1570579 ID=MS.gene86368.t1.exon15;Parent=MS.gene86368.t1
chr2.3 CDS 1570579 1570602 1570579 ID=cds.MS.gene86368.t1;Parent=MS.gene86368.t1
Gene Sequence

>MS.gene86368

ATGGCGAACGAAGATTTATCCAGTTGGACCGATCTACTTCACTCTTCCTCCAAGCTTCTCGAACAAGCCGCTCCTTCCGCTCAGTTCCCTCCTCTTCAGGTTCTCTCAATCGCACCATTTTTTCACTCTTCTTTTTTTAGGTTAAGTTTATATGCATTTTGATTTTCAACTATGCTTAGCTTCAATTCGATTGAATAATTGAATAATTATTACTTTCTGTTAGTTAGAATTGTTTTTGACTGAAAATTGAAATAATTATTGATTTTTACTACGATTTTGGGGATTTATTATTTTCAATGTTGTTTAGAGGAACTTGGATCAACTTGAAGCTTTATCGAAGAAGCTTAAATCCAAGACTGTAAGAGCTGAAGCGCCGTCACAGTCCATTGCTGCAACTAGGTACTCTTTAGTTCTTGTTAATGCGAAATTTATATTCTGTTCATAAGTTGTTCTATTATGTTTTGGTAAGTTTCTAGTTTAGTTGGATTGCTTTTAGTGCATGTTTGGTTTTGCCGTGACAAAAATTGATTTTGTAAAATTGATTTTACTTAAAAGTGAGTTGAAGGATTGTAAAAGGATTTATGTTTGGTTGGATACATTTAGGTAAAAGTGAGTTGAAAAATAAATTTCAGTTAAACAACACGTTTAGAATTAAAGCTAAAAATCCTGGATTTAAGATAGAATCAATTATGGAGGCAAAATCAATTTTACTCAAGAGCAACCAAACATGTCAAAATCAATTTTACACTTTAGAATCAATTTCTTTGATTATGATACATTATTATTCTTTCAAATGCTTTTTTTGCTTTCTCAGTAGGGGCATTGCTTTTGTTTTACATATTTCTAATAGGGCATGTGGAGCCATCATACATGTCCTAATAGACTGCCTTCCATCATTTTCTTAAAGCTAAAGTTACTCGAATTCACGACAGCATATAGGCAATTAGTGTAATAATTTCTGTTATTTATTTTGCACTCACATACAGGCTTCTTGCTCGTGAGGGTATAAATGCAGAGCAGCTTGCAAGGGATTTGAAGTCATTTGAGTTGAAGGTACCACTTAACATATATTTTGCAATCATTTTTACATTTAACATCTTATTGTTGTTGCTTTGCGTACCATGTGCGTCTTGTGCGGGTATTGCTTAATAAAATTTGCCGTTTCAAAAAAAAAAAAAACATCTTTACCTTTTCTTGGATTGTACTATTCTATTTATTTGCCCTTACACTATAATTTATGCACAACAAATTAGTTTTCATTATTTTTGAGCTGCCATACTTAAAGTTGCACAAACTTCCTTGTTTTCATGATATAACCTATTTGGATAGAATTCCTTGTATAAACTTTCCTGATGTAACCATACTTTAAGTTGTATAGACATCCTTGTTTTCCTGATATAACCTAACTGGATGTAAACTTAATTTTGTTGGAAGTTGACGTCAAATTACAAGCTTAGTTGCTCTTGTTGGGATTTAAATTCTTCAACTCATATATTTTTTTGCTAATGCTGTGCCCTTTGCCTGTTTTTGTTGTTTTGTAAAGACAACTTTTGAGGATGTTTTTCCTGTTGAGGCAACAAGCGTCGAAGAGTATCTGCAGCAGGTAATTTCTACTTTCAAATTGCTTGTTATCCATCTTGGATTTAGTTATCACTTTAAAATCATTATTAGACATCAGTTTGATTCAATCTGAATAGTTTTTATGCGTGTTAGATATCATGTACTCTAAGTCTACCTTTTTGAGTTTGCTTTCATTTTTCTCCAGCAAAAATGTTCACAAAAATCAATGAAAACTATAATGTCGGAGTTCTGAATAGGATGTTTGATTTTTTCAATAAATCACTTGTCAAAGTTAAAGAGCTGTCCCCTGAACTATCTTAAAAAAAAAACAGCTGTCCTTTGTTGATGTGCGTGGTATTGTAGCATGTTCCCCCTTATTTTTTATGAGCATAGCAAAATTAGTTCAGTGTTTGTATTTGAAGTTATGTACCTTGCTATGGGAAACCAATTAAGTCTCCTTACTCTTCACTTGACTTGGTCCTTTCTGAATAGAATTGTGACAATATTGAATACTCTTTTTCGTTCTCAGGTTCATGAAATGGCAATGGTCTCTGCCGTCCAGGAAGCTCAGAAGGATAACCTTAGGAGTTTCAATGATTATATGATGAAAGTTTTGGAGGTTAGTGTAATCTTGCCTTTCATGTCATGGCACACCATGATTTTTTCTGTATTCTGTGTAATTATTGGTTATTACTGGAGAAATTTTTGTAATGTGTTTTGCTAAGCTATTTATAAAATAAGAGTTATTGGGGTAAGATTTGGTAAACCATTAATTTGGATACTCAATGTGTCCTGTCTTTTTCTTGCTTTGCTGAAAATAAGATATCTTATGGATGTGCCTATTTTATTTTCTTTATTTATTTGATTGTAGTCAAAAGCCTTAAACTTTCAAAAGGAAATATCAGTCCTTATATTTTTGTGTGGATATGGGTACAAGATTATGACAAAGGAATTGATGGGATTTATTGAACTATAATAAGGTTGGTTGTTTTTGCATACTTTTTGATGAGGATATATCCTTTTTCGGTGAACCTTGTCTGTAATCCTTGTTTCCTCTGTGTATGAAAATTTCTCACATTCTTTTAGAAAATCCAATATTAAAAATGAATAGAAAACAAAATATGTAAAGCGTAGTTTTTGTTTAATTTATACAACTCGATAGTTTTCCATAATTTTGACTTTGGCAACGAGGTTTGCTGACCTACTTAAACTTCTCACCAATCAAATCATCCCATTTGGCTGATTGATTGTTTTTCTATAAAATATGAAATGAAGTAGCATAGTGAAGTAAGTAAATCACTATATAATATATATCATATTTGCAATTTTATAATTGGGATGAGTATTTTATTGCATTGTTATATTGGTCATAAAAATATGTTTATTTGTTCTTGATATTATGTGGCTCATCCTTCTTACTGCTCAGTAGCATTTAATGTTTTTGACAGGAGGATTGGCAAAAAGAAAAGCGTGATTTCCTTCAGAGTTTAAGCAGGATTTCAACATTACCAAGGACAAACATGATTGCTAATAGCAATGTGGGTGCCCGCCCTGGTCAAATTGTTTCTATGGTTTCTACTCCACAAGTCTCTTCTGGTAGCATGGAGATTGTTCCCATGACTAGTAGACCCATCGCAGATAAAAAGGCATCTGTCTATGCTGAAGTAGTTAAAAATCTGAATAGAGCAAGGCAGTCTGGATTGCCATTTAAAGTAAGTCCCCTACTTTCAGAGCTCTATATTAGATCAAAAACCTAGTAGATGGTGTAGCCTGTTCATGACTTAAGTGATCGTGTCTGGAACCCAAATTCAAGAGAAATAGAAATTGTATTATTGATTGGAATAAGGAGAACTATTACAGAGAATGATCCCTATACTCCCAGAGGAAAAGCTCCTCAGAGAAGAGACATTTCTCTCTCTCTGCCCAAATTACAAGATTCTAACTAAATAATGGGAATACTCCTCTCAATTGCTATCCCTATCCTTTATATAACTAACATTTCTCTAACAAACTAGCCCCCTCACCCAATGTAACTAACTCTCCTAGTAAGCACATTAGTTGGGCTTGAGCCCAAACTCAGTACCTAACAGATCAACATTGTGATTTGCATGAAGCTAATGTATAAACTTTTAATTCAATAATGTTCCAACAACATTTTGAACATAAGCAATGCTTTCTTGTTTTTGAGATCCTTTCCCAAAAGTTCTTACTAGTATTCACCTTTTTGTATTTATATGTATGTACGCTAATTTTCATAATAATTTATCTATGCTTGATAGCTTGCCGCAACTTTCAAGGGTGCATACGAAAGTTTGGGCGTTGATGCTGGTGGTGGAAAATCAGTAACGATGCGAAAGATATGGCACCTTGTTCAGGTTGCACTTGTTGTTGTATACTGTTAACAGGCAAAACAGAGAGAAACTTTTGCCTGCAACTTTATTGGTTCTACCTGTTATGAAGTAAACATTTTTATTTATTTTCTAGACTTGGCTAATTGAAACAGATGCTGATGGATGAGGACTCAACCTTGCGACGTGTTTCCAAAAGAATGTCATTGATTATTGGGGCAAGGCGCCACCTTGAGTGGGGACATGAGAAATACATCATGGATACTATACATAATCATCCCGCACAAGTAATGTGTTATTCAGAATTTCAAGTGGTTTATTCATATGAGAGCATGCATTGATTTTTATTTGTTGTATCAACACTTAATTTCTGACATCTTTCTTTTATCTAATGTAGGCTTCTCTTGGTGGTGGTGTTGGAAACTTGCAAAGAATCCGCGCCTTCCTTCGGGTTCGTCTCAACTTGAAGAATATGCGTCTTGTTAAACACTTTACCATGTATTTAATTTTTTTTTCCTTCAAAAAATCCCTGATTTTATTGCTGCGCAGATTCGGTTGAGGGATTATGGGGTTCTGGATTTTGATGCTGGTGATGCTCGGAGACAGCCACCTGTTGATACCACATGGCAACAGGTCTTGACTACTTTATCTTACTATAAAATTATTAGGGACGAGACTTGAAATTGAATCCGAAGGGTGCATACAAATTACTCGCCATGAGTACGATTTTTAGTCATCAAATGGATTTGGGTCTAGGTATGGGTAATATTAATACATATAAAAAATTGTGGGCATTTCCATGGGGCATGGGGGCCATATGTTTTATTATATTTTATCCAAACGATATGCTTCATATATTTTATAACTAGCCGTAACAGTAATACCCATGCTTTGTATATGTTACTTTTGAATGGAGTAAATTCCGTTTTTTTATTATATGTTAATCATCTTGTAAAGAATTTAGAGTGAAAGAAAAAAAGGTTCCGGGCCTCCAAGAGTATGTGAAAAAATCTTTAATGCAAAAAAGTATCTATAAAAACTACGGGTATAAGTATCTAAACCTACATCTTACCTGCACAATATGCTCATCATATTTTATAACAACACGTTATACCCTTCCTTTGTATGGGTTACTTATAAATGAAACAAGAATTCTGTTTAAAAAAGGGCAACCCGGTGCACTAAAGCTCCCGCATACGCAGGGTCCGGGAAGGGAAGTCGTGGTACTGTTGGGATATGTAGACAGATTTATAGAACTCTATAGATTAGGATAAGATTTCAAATATCTCTCTGGATATTTTTTAATGAAGTTAGATTTTACACTTGTCAATGCTATTTTTGTTGACTTATCATATACTCAAATAAATTTACCAGTATCTTAAAAGAAAAAGATACATCAATGGAAACAAATCCCATAACCACAACATGAGTTTTGTTTGCTTTCATATCCGAGTATATAGGCATCTATTAGGTACGGTTAAGGGCCTCCAAGTTGCTACCCGCATGAGTTTGGGTTTGTGGCAATTTCTTAAATGTGGATATGGATATTCTAGTTTTTTACATAGACTCCACGCTTTGCTATCCCTACAATTTAGTTACACTTCATTAACAGAGTTCAGTCCTATATCTAATTTTTTTGTTGCTTAAAATCAATATTAATGATGAGCTAAGCTTGTACTTTCTTTTTTTAACAGATATATTTTTGTCTGAGGTCTGGTTATTACGATGAAGCAAGAAATGTTGCTCTATCTTCGCGTGCTTCACATCAATTTGCTCCTTTGGTACATATACATAGTGTGATCACTTCTTGTTATGTGCTTATTTATAGTTCACTTTTAATAATTTACTGTTTCTTACAGCTCACAGAGTGGATTAATACTGGAGGAATGGTGCCAGAAGAGGTGGCTACTGCTGCATCAGAAGAATGTGAAAGAATGTTGAGAACTGGTGATCGGGTAGGCCGGACTGCATACGACAAGAAAAAATTACTGCTGTACGCCATCATATCAGGTTCTCGAAGACATATTGATCGCCTGCTTAGAGATCAACCAACTCTTTTTAGCACGATAGAGGATTTCTTGTGGTTCAAATTGTCGGCTGTGCGGGACTGCCCCAGTGGATCTTCGTCCATTGTTCTAAGTGATGGTCTGATTCCATACAGTTTGGATGATTTGCAAAGTTACCTAAATAAATTTGAACCATCATACTATACAAAAAATGGAAAAGATCCCCTGGTATATCCCTATATCTTGCTTTTAAGCATTCAATTGCTTCCGGCTGTATTATACTTGTCTAAGGAAGCAGGAGATGAAGGATATAACATTGATGCTGCCCACCTATCAATTGTGCTAGCCGATCATGGCGTCCTTTCTGAAGGCATCGGTACTGGGCAAAAGTTGGGAGTGATGGATGCTTATGCAGAAGTTTCCACAATTATCAGGCAATATGGATCTATGTATTTGCGTCTTGGTGATCTTCAAATGGCACTAGAATATTATGCACAAGCTGCTGCTGCAGTCGGCGGTGGACAGTTGTCGTGGACTGGTAGAGGTAATGTTGATCAACAAAGGCAGAGAAACTTGATGCTGAAGCAGCTTCTAACTGAATTATTGTTAAGGGATGGAGGTATATATCTTTTACTTGGTGCAAGAGGTGCCGGAGAAGAAGGAGAATTGGGACGTTTTGTAGCGGATCCAAATGCAAGGCAACAATTTTTAATTGAAGCGGCATGCCAGTGTCAGGAGGCTGGGATGTATGACAAAGTAATTTTTTTCTCATGCTACTCTTTTATTTTAGAGGAACAATAGGGAAAGTAAGAAAACCTTCAACTGCTTTTACTGAATTACAACATTACCTAGCTCACAATCATTGTTCTTCATGCCCTTATTGCGGCACTTAAATATGGAAATTACTCCACTCAGTTTTTAGACCTTTGTTTTGGCCTCTTAGACATCCTTTTTCTGGTGAAGCTAAAGATATTTTTAACACCATCTAGGAATTTTGTTAACTCTTGTTTATTTTCAATTTCTAATACCTTTTTGATTACATTTCATTATTTCTCACATCTGTGTCTCTCATACTGTGTCTCTACTCTCTAGATTTTTCCCACCTAGGTTATTTAAACTTATTTTCTCCTAATTTTTGTTACCTCCGTGTTCCCTTATTCATCACCATTTGTGAGTAAGTTTTTTCATCCAACCTTTATGTTCCTTTTGCTGATGCGGAATTTTTTGGTTCAGTCTATAGAAATTCAGAAGAGAGTTGGTTCCTTCTCAGTGGCGCTTGATACCATCAACAAGTGCCTCTCTGAAGCTATTTGTTCTCTTTTCCGTGGAAGGTTAGATGGTGAGAGCCGGACTTCTGGCCTTATCCATTCTGGCAATGAGATTTTGGAGACATATACTTACTACCCAGATGTCAGGTGAAATTATTACCTTTTATTTCCTTGCTCACATATTTCTCTGCTTGGACTTTGGGATTGTGATATTCTTAAACTTTGCATGATATTTCAAAAATGAACCTTTTCAAGTTGTGGTGATTTGACACAGATTATGCTTTGGTTGTTATTTTCATCTTTTCCCTTATCTTTTTTCATGGAATGATCTTTGAGGTCAAACCCTGCTTGTGTGTAACTTGGAAGTCCATTTTCGCCATATCCTTACTTCACATGGCTATTGGTGGTAATTTACATGCAAAAACAGGTGGCACAAAGATGAATGACGGGTGATATGGGATATACCATTCACAGGATTACGCCATCTTTTTGCTGATACTACGGTTGTAGTCTTTGTTAGGTGTTGGTTTTGTTTTGTTTTTTCTGCTGTACCAAAAAAAGCTATCAGCCTAATCAATTTGTAACTTTCTTCATAAAATTTGTCAGTTTTGGCTCTTCAAACTAAATATCTATGGAAGTTTCTTTCAAGTTTCAACTATGAAGAATTTGTTGTTTATCAATCTATAGCACAATCCTTAAGAAAAAAAATGATCAATTGAATTCTTTTTGCAGTCATCAAGAGAGAGAGCAAGTTTTTGAGCAGCAAACTGTATTGAGACAACTTGAGTCAATATTATCAATTCACAAATTGTCAAGACTGGGGAATCATGTTGATGCATTAAGGGAGGTTGCTAAACTTCCATTTTTGCCTCTAGATCCCCGAGGACCTGATATTGCTGTTGATGTGTTTGAAAATTTGTCTCCTCATGTCCAAGCTTGTATACCAGACCTCTTGAAGGTTGCTCTGACTTGTCTGGACAATGTGACAGACTCTGATGGATCACTTCGTGCTTTGAGAGCTAAGGTAATATCCCGTGGATTCCCTTCTTTGGTTGGTCTATATCCATGCTTTAGTTGCTTTTGTTTTTTTACCATAATTTACTATAACTGTTCTCAACAAGTATTTGGAGCACTGCCAGAAGTAA
Protein sequence

>MS.gene86368.t1

MANEDLSSWTDLLHSSSKLLEQAAPSAQFPPLQRNLDQLEALSKKLKSKTVRAEAPSQSIAATRLLAREGINAEQLARDLKSFELKTTFEDVFPVEATSVEEYLQQVHEMAMVSAVQEAQKDNLRSFNDYMMKVLEEDWQKEKRDFLQSLSRISTLPRTNMIANSNVGARPGQIVSMVSTPQVSSGSMEIVPMTSRPIADKKASVYAEVVKNLNRARQSGLPFKLAATFKGAYESLGVDAGGGKSVTMRKIWHLVQMLMDEDSTLRRVSKRMSLIIGARRHLEWGHEKYIMDTIHNHPAQASLGGGVGNLQRIRAFLRIRLRDYGVLDFDAGDARRQPPVDTTWQQIYFCLRSGYYDEARNVALSSRASHQFAPLLTEWINTGGMVPEEVATAASEECERMLRTGDRVGRTAYDKKKLLLYAIISGSRRHIDRLLRDQPTLFSTIEDFLWFKLSAVRDCPSGSSSIVLSDGLIPYSLDDLQSYLNKFEPSYYTKNGKDPLVYPYILLLSIQLLPAVLYLSKEAGDEGYNIDAAHLSIVLADHGVLSEGIGTGQKLGVMDAYAEVSTIIRQYGSMYLRLGDLQMALEYYAQAAAAVGGGQLSWTGRGNVDQQRQRNLMLKQLLTELLLRDGGIYLLLGARGAGEEGELGRFVADPNARQQFLIEAACQCQEAGMYDKSIEIQKRVGSFSVALDTINKCLSEAICSLFRGRLDGESRTSGLIHSGNEILETYTYYPDVSHQEREQVFEQQTVLRQLESILSIHKLSRLGNHVDALREVAKLPFLPLDPRGPDIAVDVFENLSPHVQACIPDLLKVALTCLDNVTDSDGSLRALRAKYLEHCQK