AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86982

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86982.t1 MTR_2g104490 91.003 289 22 4 1 288 1 286 0.0 524
MS.gene86982.t1 MTR_2g104490 91.003 289 22 4 1 288 1 286 0.0 524
MS.gene86982.t1 MTR_2g104550 79.932 294 49 6 1 288 1 290 2.03e-155 437
MS.gene86982.t1 MTR_4g009540 42.249 329 129 10 1 288 1 309 1.13e-65 208
MS.gene86982.t1 MTR_4g009540 42.249 329 129 10 1 288 1 309 1.95e-65 208
MS.gene86982.t1 MTR_4g009110 42.433 337 136 10 1 288 1 328 7.74e-64 205
MS.gene86982.t1 MTR_8g009020 48.649 222 101 6 76 288 84 301 3.52e-51 172
MS.gene86982.t1 MTR_7g080780 36.792 318 160 6 1 288 1 307 1.44e-48 165
MS.gene86982.t1 MTR_2g104500 40.741 270 133 6 23 288 4 250 2.72e-48 162
MS.gene86982.t1 MTR_7g080780 36.877 301 151 5 18 288 3 294 1.04e-46 160
MS.gene86982.t1 MTR_8g069740 39.146 281 141 10 25 292 6 269 5.19e-44 151
MS.gene86982.t1 MTR_8g009010 39.855 276 112 9 31 288 11 250 3.98e-41 145
MS.gene86982.t1 MTR_2g104530 47.283 184 91 3 108 288 72 252 5.25e-40 141
MS.gene86982.t1 MTR_5g014600 38.676 287 126 11 41 288 38 313 6.47e-39 140
MS.gene86982.t1 MTR_5g014520 41.441 222 101 5 81 288 102 308 7.19e-39 139
MS.gene86982.t1 MTR_6g047570 39.300 257 129 9 44 288 69 310 8.61e-36 131
MS.gene86982.t1 MTR_0246s0020 38.819 237 119 6 81 292 122 357 5.37e-35 130
MS.gene86982.t1 MTR_0246s0020 39.056 233 116 6 81 288 122 353 1.60e-34 129
MS.gene86982.t1 MTR_4g067010 40.741 189 105 3 105 288 109 295 4.25e-34 127
MS.gene86982.t1 MTR_0250s0040 42.690 171 92 4 122 288 71 239 1.93e-33 124
MS.gene86982.t1 MTR_0011s0260 40.196 204 110 4 96 288 132 334 2.83e-33 125
MS.gene86982.t1 MTR_2g104560 42.857 175 86 3 121 288 36 203 1.20e-32 120
MS.gene86982.t1 MTR_4g066460 37.083 240 132 6 58 288 74 303 1.23e-32 123
MS.gene86982.t1 MTR_2g010450 46.875 160 76 3 135 288 173 329 2.99e-32 122
MS.gene86982.t1 MTR_4g092700 40.217 184 106 2 109 288 126 309 7.41e-32 121
MS.gene86982.t1 MTR_0011s0210 40.196 204 110 4 96 288 132 334 8.78e-32 121
MS.gene86982.t1 MTR_5g014640 34.043 235 143 5 69 299 87 313 2.45e-31 119
MS.gene86982.t1 MTR_4g097940 43.023 172 88 4 122 288 138 304 1.48e-30 117
MS.gene86982.t1 MTR_4g097920 42.442 172 89 4 122 288 123 289 7.79e-30 115
MS.gene86982.t1 MTR_4g097920 42.442 172 89 4 122 288 138 304 9.97e-30 115
MS.gene86982.t1 MTR_2g104650 33.818 275 142 9 23 288 4 247 1.46e-29 114
MS.gene86982.t1 MTR_5g014560 42.690 171 92 3 122 288 114 282 2.48e-28 111
MS.gene86982.t1 MTR_4g097950 38.265 196 109 2 105 288 33 228 7.67e-28 108
MS.gene86982.t1 MTR_2g104590 36.190 210 109 6 84 288 13 202 1.03e-27 107
MS.gene86982.t1 MTR_4g066380 36.325 234 131 6 58 288 82 300 2.91e-27 108
MS.gene86982.t1 MTR_0246s0050 38.182 165 93 3 126 288 138 295 1.34e-26 107
MS.gene86982.t1 MTR_4g098035 38.182 165 93 3 126 288 159 316 1.57e-26 107
MS.gene86982.t1 MTR_7g451650 43.077 130 60 2 162 288 57 175 4.59e-22 91.7
MS.gene86982.t1 MTR_7g451650 43.077 130 60 2 162 288 57 175 6.81e-22 91.3
MS.gene86982.t1 MTR_7g050350 41.935 124 58 2 168 288 1 113 3.75e-19 82.4
MS.gene86982.t1 MTR_7g451650 41.935 124 58 2 168 288 1 113 3.75e-19 82.4
MS.gene86982.t1 MTR_4g009420 39.370 127 61 2 162 288 65 175 4.40e-17 78.2
MS.gene86982.t1 MTR_4g009420 39.370 127 61 2 162 288 65 175 7.63e-17 77.8
MS.gene86982.t1 MTR_4g009510 38.462 143 72 4 150 288 53 183 1.32e-16 77.0
MS.gene86982.t1 MTR_8g067280 25.781 256 156 6 45 273 361 609 1.55e-15 77.4
MS.gene86982.t1 MTR_6g047550 46.835 79 42 0 139 217 173 251 2.52e-15 74.7
MS.gene86982.t1 MTR_5g030430 25.431 232 148 4 66 273 410 640 3.37e-15 76.3
MS.gene86982.t1 MTR_8g024790 29.310 174 108 6 106 273 264 428 7.27e-15 75.1
MS.gene86982.t1 MTR_5g014550 39.516 124 69 3 169 288 54 175 1.42e-14 71.2
MS.gene86982.t1 MTR_8g027495 31.496 127 78 4 138 264 457 574 6.39e-13 69.3
MS.gene86982.t1 MTR_7g117670 34.211 114 61 2 138 247 401 504 1.99e-12 67.8
MS.gene86982.t1 MTR_1g072320 25.949 158 107 2 123 270 454 611 8.43e-12 65.9
MS.gene86982.t1 MTR_8g024770 25.556 180 113 7 118 288 262 429 3.03e-11 63.9
MS.gene86982.t1 MTR_7g096350 26.531 147 85 3 128 273 343 467 9.44e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86982.t1 AT4G37850 39.604 202 106 5 96 291 116 307 8.41e-34 126
MS.gene86982.t1 AT4G37850 39.604 202 106 5 96 291 116 307 8.41e-34 126
MS.gene86982.t1 AT2G22750 37.611 226 120 6 77 289 65 282 1.06e-29 114
MS.gene86982.t1 AT2G22750 37.611 226 120 6 77 289 93 310 1.43e-29 115
MS.gene86982.t1 AT2G22750 37.611 226 120 6 77 289 89 306 1.48e-29 115
MS.gene86982.t1 AT2G22750 37.333 225 120 6 77 288 65 281 4.86e-29 113
MS.gene86982.t1 AT2G22750 37.333 225 120 6 77 288 93 309 6.38e-29 113
MS.gene86982.t1 AT2G22760 41.176 170 95 2 131 295 111 280 2.35e-28 110
MS.gene86982.t1 AT2G22760 42.331 163 89 2 131 288 111 273 2.85e-28 110
MS.gene86982.t1 AT2G22770 37.725 167 87 2 139 288 132 298 4.74e-23 97.1
MS.gene86982.t1 AT5G46760 28.800 250 148 9 40 273 320 555 6.99e-17 81.3
MS.gene86982.t1 AT4G17880 27.650 217 141 5 62 273 347 552 1.80e-16 80.1
MS.gene86982.t1 AT4G09820 33.803 142 76 2 129 270 353 476 4.19e-16 79.0
MS.gene86982.t1 AT2G46510 36.800 125 62 4 122 245 377 485 1.95e-14 73.9
MS.gene86982.t1 AT1G32640 28.652 178 108 7 128 290 440 613 5.73e-13 69.7

Find 57 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTCTTCCCTTCCTCCAAC+TGG 0.191139 2.3:+252025 None:intergenic
CAGCAACCTGTTACATATTC+TGG 0.210205 2.3:-254429 MS.gene86982:CDS
GCTAGCAGTGTATTGGCTTT+TGG 0.252967 2.3:-253510 MS.gene86982:CDS
GATTCTTTGCTATTCTTGTT+TGG 0.275304 2.3:+253738 None:intergenic
TTACTTCTACTCTTTGTTCT+TGG 0.277856 2.3:+254240 None:intergenic
ACTTCTTGTTCTGATTCTTC+TGG 0.307827 2.3:+254264 None:intergenic
TGATGTTAAAGCAAGAATAT+TGG 0.324656 2.3:-253628 MS.gene86982:CDS
GCACTTTCAGCTACTATTCC+TGG 0.324686 2.3:-254084 MS.gene86982:CDS
AGTAGTAGTAGCTGGTATTA+TGG 0.354699 2.3:+254290 None:intergenic
TAGAAGTGGTTCTCAGTGTT+TGG 0.364117 2.3:-254160 MS.gene86982:CDS
TGTCACTGCTAGCAGTGTAT+TGG 0.365840 2.3:-253517 MS.gene86982:CDS
TGAGCCATCCATCTCATTAG+AGG 0.377502 2.3:+254392 None:intergenic
AAGTAATAATAAGAGGAGTT+TGG 0.391916 2.3:-254223 MS.gene86982:CDS
TCAGTGTTTGGATCACATTA+TGG 0.401190 2.3:-254148 MS.gene86982:CDS
CTGCTTCCAGAATATGTAAC+AGG 0.414669 2.3:+254423 None:intergenic
AGAGTAGAAGTAATAATAAG+AGG 0.424376 2.3:-254230 MS.gene86982:CDS
TGGCTTTACTATTGTCGCTC+AGG 0.443880 2.3:-253475 MS.gene86982:intron
GGAACATTGGCCTCTTCATT+CGG 0.453854 2.3:-254685 MS.gene86982:intron
CTTCTTCCCTTCCTCCAACT+GGG 0.463799 2.3:+252026 None:intergenic
AGAACATCATGGAGGAATCA+TGG 0.464890 2.3:-254707 None:intergenic
TTCTACTCTTCTTTCCACTT+TGG 0.465419 2.3:+254178 None:intergenic
TTTATTACCTTCTTCAAGCC+AGG 0.465921 2.3:+254066 None:intergenic
AAAGATAGAATAAATGTCCT+TGG 0.468384 2.3:+254324 None:intergenic
AGCAAGAATATTGGAAAATG+AGG 0.474113 2.3:-253619 MS.gene86982:CDS
TAATGGTGACAATAGGAATT+GGG 0.480317 2.3:-254531 MS.gene86982:intron
TACCACCTCAAATTCTGATC+AGG 0.481127 2.3:-253673 MS.gene86982:CDS
ATGGATGGCTCAGATTGCTC+AGG 0.485183 2.3:-254381 MS.gene86982:CDS
CCAGTTGGAGGAAGGGAAGA+AGG 0.509133 2.3:-252025 MS.gene86982:CDS
TGGAGGAATCATGGGAACAT+TGG 0.515296 2.3:-254698 MS.gene86982:CDS
TACTATTCCTGGCTTGAAGA+AGG 0.517604 2.3:-254073 MS.gene86982:intron
TCTGGTGTAGTAGTAGTAGC+TGG 0.520741 2.3:+254282 None:intergenic
TCATAACCCAGTTGGAGGAA+GGG 0.521814 2.3:-252032 MS.gene86982:CDS
ATAGGAATTGGGTGATGTTG+AGG 0.530496 2.3:-254520 MS.gene86982:intron
GCAATCCTGATCAGAATTTG+AGG 0.532272 2.3:+253668 None:intergenic
GTCATAACCCAGTTGGAGGA+AGG 0.543020 2.3:-252033 MS.gene86982:CDS
AAGAGTCATAACCCAGTTGG+AGG 0.557437 2.3:-252037 MS.gene86982:CDS
TCTTTCATCCTCTAATGAGA+TGG 0.562481 2.3:-254400 MS.gene86982:CDS
AGTGGAAAGAAGAGTAGAAG+TGG 0.563816 2.3:-254174 MS.gene86982:CDS
GGTGATGTTGAGGATTTGCT+AGG 0.567202 2.3:-254510 MS.gene86982:CDS
TAGAATAAATGTCCTTGGAG+TGG 0.581040 2.3:+254329 None:intergenic
ATGGACAAGAACTATATACT+TGG 0.584966 2.3:-253816 MS.gene86982:CDS
GCAAAAGCAAGTAACCAAAG+TGG 0.587147 2.3:-254192 MS.gene86982:CDS
TGGACGGTGTTGATGAAGGA+TGG 0.592345 2.3:+254349 None:intergenic
GGAGTGGACGGTGTTGATGA+AGG 0.594219 2.3:+254345 None:intergenic
GAACATCATGGAGGAATCAT+GGG 0.596545 2.3:-254706 None:intergenic
ATAAATGTCCTTGGAGTGGA+CGG 0.600736 2.3:+254333 None:intergenic
CATTCAGATCCACTGTGAGA+AGG 0.601974 2.3:-253592 MS.gene86982:CDS
TCATCCTCTAATGAGATGGA+TGG 0.603818 2.3:-254396 MS.gene86982:CDS
CTTGCTTTAACATCAAAGAG+TGG 0.605135 2.3:+253636 None:intergenic
AATATGTAACAGGTTGCTGA+AGG 0.616197 2.3:+254433 None:intergenic
CATCAACACCGTCCACTCCA+AGG 0.620000 2.3:-254341 MS.gene86982:CDS
AGAACGTGTGAAGGAGCTAG+AGG 0.643700 2.3:-253763 MS.gene86982:CDS
GTGATGTTGAGGATTTGCTA+GGG 0.643753 2.3:-254509 MS.gene86982:CDS
ACTACTTCAAGAACGTGTGA+AGG 0.653803 2.3:-253772 MS.gene86982:CDS
TGGATGGCTCAGATTGCTCA+GGG 0.676528 2.3:-254380 MS.gene86982:CDS
TGTCAATTTCCTTCTCACAG+TGG 0.679003 2.3:+253583 None:intergenic
ATCCTGATCAGAATTTGAGG+TGG 0.691103 2.3:+253671 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAAAAAACAAAAAAAAA+AGG + chr2.3:254353-254372 None:intergenic 10.0%
!! AGAAAAAAACAAAAAAAAAA+GGG + chr2.3:254352-254371 None:intergenic 10.0%
!! TAAATAATCATTTCAATTAA+TGG - chr2.3:252172-252191 MS.gene86982:intron 10.0%
!!! TATAATATCATAATGTTTTA+TGG + chr2.3:253297-253316 None:intergenic 10.0%
!! AATGTTTAGCTATTTAAATA+TGG + chr2.3:253618-253637 None:intergenic 15.0%
!! ATGTTTAGCTATTTAAATAT+GGG + chr2.3:253617-253636 None:intergenic 15.0%
!! CAATCTAATATTTAATAACA+TGG - chr2.3:254017-254036 MS.gene86982:intron 15.0%
!! TGCATAAATTAATTAATAGA+TGG - chr2.3:252885-252904 MS.gene86982:intron 15.0%
!!! AATTATTATTTGAAGAGTTT+TGG + chr2.3:254644-254663 None:intergenic 15.0%
!!! AATTATTTTAATCAAGTTGT+AGG - chr2.3:253764-253783 MS.gene86982:CDS 15.0%
!! AAACAATATTAGAAAAACCT+CGG - chr2.3:254268-254287 MS.gene86982:CDS 20.0%
!! AGACGTTTGATAAATTTATA+AGG + chr2.3:253392-253411 None:intergenic 20.0%
!! CTCATGAGATAATATTTAAA+GGG - chr2.3:253410-253429 MS.gene86982:intron 20.0%
!! TATTAATGAATAGTTTGACT+TGG + chr2.3:253713-253732 None:intergenic 20.0%
!! TCTCATGAGATAATATTTAA+AGG - chr2.3:253409-253428 MS.gene86982:intron 20.0%
!! TGAAGAAAATTACTCTAATT+GGG + chr2.3:254498-254517 None:intergenic 20.0%
!! TTGAAGAAAATTACTCTAAT+TGG + chr2.3:254499-254518 None:intergenic 20.0%
!!! ATGGTAATAATTCACTTTTA+TGG - chr2.3:253552-253571 MS.gene86982:CDS 20.0%
!!! ATTAAGAGCACTAATTAAAT+TGG + chr2.3:252694-252713 None:intergenic 20.0%
!!! ATTTTATAACGGATTGAATT+GGG - chr2.3:252847-252866 MS.gene86982:intron 20.0%
!!! TATTTTATAACGGATTGAAT+TGG - chr2.3:252846-252865 MS.gene86982:intron 20.0%
!!! TCAAAAACAGTTTTCTTTTA+TGG - chr2.3:253533-253552 MS.gene86982:CDS 20.0%
!!! TTAAGAGCACTAATTAAATT+GGG + chr2.3:252693-252712 None:intergenic 20.0%
!!! TTTCTAATATTGTTTTTCCT+CGG + chr2.3:254263-254282 None:intergenic 20.0%
! AAAAAAAAAAGGGATTGAAG+TGG + chr2.3:254342-254361 None:intergenic 25.0%
! AAAGATAGAATAAATGTCCT+TGG + chr2.3:252399-252418 None:intergenic 25.0%
! AACATAAAAAACTCAACCTA+TGG - chr2.3:254604-254623 MS.gene86982:intron 25.0%
! AAGTAATAATAAGAGGAGTT+TGG - chr2.3:252497-252516 MS.gene86982:intron 25.0%
! ACTGGTTAAAGATATGAAAA+AGG - chr2.3:253458-253477 MS.gene86982:intron 25.0%
! AGAGTAGAAGTAATAATAAG+AGG - chr2.3:252490-252509 MS.gene86982:intron 25.0%
! ATGAAGAAGAAGAAAAAAGA+AGG + chr2.3:254319-254338 None:intergenic 25.0%
! GAGTATATTTCTTCAATCAA+AGG - chr2.3:254431-254450 MS.gene86982:CDS 25.0%
! TGAAGAAGAAGAAAAAAGAA+GGG + chr2.3:254318-254337 None:intergenic 25.0%
! TGATGTTAAAGCAAGAATAT+TGG - chr2.3:253092-253111 MS.gene86982:intron 25.0%
! TTCATGGAAAATTCAATTGA+AGG - chr2.3:254564-254583 MS.gene86982:intron 25.0%
! TTTATGTCAGAAAATAGTAC+TGG + chr2.3:252156-252175 None:intergenic 25.0%
! TTTCAATTAATGGTGACAAT+AGG - chr2.3:252182-252201 MS.gene86982:intron 25.0%
! TTTCCATGAAGAAAATTTAC+TGG + chr2.3:254554-254573 None:intergenic 25.0%
!!! TATTATTTGAAGAGTTTTGG+AGG + chr2.3:254641-254660 None:intergenic 25.0%
!!! TGTTCTATGTCATTTTTTTG+AGG - chr2.3:252104-252123 MS.gene86982:intron 25.0%
!!! TTGGCTACTATTTTATTTAC+TGG + chr2.3:253694-253713 None:intergenic 25.0%
AGAAGAACATATTAATCCCT+CGG + chr2.3:252795-252814 None:intergenic 30.0%
AGCAAGAATATTGGAAAATG+AGG - chr2.3:253101-253120 MS.gene86982:intron 30.0%
ATGGACAAGAACTATATACT+TGG - chr2.3:252904-252923 MS.gene86982:intron 30.0%
ATTTAAAGGGAAATGCTAAC+CGG - chr2.3:253423-253442 MS.gene86982:intron 30.0%
TAATGGTGACAATAGGAATT+GGG - chr2.3:252189-252208 MS.gene86982:intron 30.0%
TACTCAAATTTACTGCTAGT+TGG + chr2.3:254416-254435 None:intergenic 30.0%
TAGTTGGATTCTGGATAATT+GGG + chr2.3:254400-254419 None:intergenic 30.0%
TTAATGGTGACAATAGGAAT+TGG - chr2.3:252188-252207 MS.gene86982:intron 30.0%
TTACTTCTACTCTTTGTTCT+TGG + chr2.3:252483-252502 None:intergenic 30.0%
! ACACCAGTAAATTTTCTTCA+TGG - chr2.3:254548-254567 MS.gene86982:intron 30.0%
! CAGTCGGATTTTAAATTCTT+CGG + chr2.3:254204-254223 None:intergenic 30.0%
!! AGTATTTTTACCGAATGAAG+AGG + chr2.3:252048-252067 None:intergenic 30.0%
!! GATTCTTTGCTATTCTTGTT+TGG + chr2.3:252985-253004 None:intergenic 30.0%
!! TTTTATTTACTGGACCTTGA+TGG + chr2.3:253684-253703 None:intergenic 30.0%
AAAAAGAGTCATAACCCAGT+TGG - chr2.3:254680-254699 MS.gene86982:intron 35.0%
AATATGTAACAGGTTGCTGA+AGG + chr2.3:252290-252309 None:intergenic 35.0%
ACTTCTTGTTCTGATTCTTC+TGG + chr2.3:252459-252478 None:intergenic 35.0%
AGTAGTAGTAGCTGGTATTA+TGG + chr2.3:252433-252452 None:intergenic 35.0%
ATATCTTTAACCAGTGTCTC+GGG + chr2.3:253453-253472 None:intergenic 35.0%
ATTTACTGCTAGTTGGATTC+TGG + chr2.3:254409-254428 None:intergenic 35.0%
CATATCTTTAACCAGTGTCT+CGG + chr2.3:253454-253473 None:intergenic 35.0%
CTAAATGTTCCATTCCATCA+AGG - chr2.3:253667-253686 MS.gene86982:CDS 35.0%
CTAGTTGGATTCTGGATAAT+TGG + chr2.3:254401-254420 None:intergenic 35.0%
CTTGCTTTAACATCAAAGAG+TGG + chr2.3:253087-253106 None:intergenic 35.0%
TAGAATAAATGTCCTTGGAG+TGG + chr2.3:252394-252413 None:intergenic 35.0%
TCAGTGTTTGGATCACATTA+TGG - chr2.3:252572-252591 MS.gene86982:intron 35.0%
TCTTTCATCCTCTAATGAGA+TGG - chr2.3:252320-252339 MS.gene86982:intron 35.0%
TTAATCCCTCGGTTAAATAC+CGG + chr2.3:252784-252803 None:intergenic 35.0%
TTCTACTCTTCTTTCCACTT+TGG + chr2.3:252545-252564 None:intergenic 35.0%
TTTATTACCTTCTTCAAGCC+AGG + chr2.3:252657-252676 None:intergenic 35.0%
! AAAGAAGGGTCTGTTTTACT+CGG + chr2.3:254304-254323 None:intergenic 35.0%
!! GTAGAAAGATTGATTTGTCC+CGG - chr2.3:252762-252781 MS.gene86982:intron 35.0%
!! TGGTTACTTGCTTTTGCTTT+TGG + chr2.3:252525-252544 None:intergenic 35.0%
ACTACTTCAAGAACGTGTGA+AGG - chr2.3:252948-252967 MS.gene86982:intron 40.0%
AGTGGAAAGAAGAGTAGAAG+TGG - chr2.3:252546-252565 MS.gene86982:intron 40.0%
ATAAATGTCCTTGGAGTGGA+CGG + chr2.3:252390-252409 None:intergenic 40.0%
ATAACATGGTGTCTTGCAGA+TGG - chr2.3:254031-254050 MS.gene86982:intron 40.0%
ATAGGAATTGGGTGATGTTG+AGG - chr2.3:252200-252219 MS.gene86982:intron 40.0%
ATCCTGATCAGAATTTGAGG+TGG + chr2.3:253052-253071 None:intergenic 40.0%
CAGCAACCTGTTACATATTC+TGG - chr2.3:252291-252310 MS.gene86982:intron 40.0%
CGAAATGACGGTGATTGATT+TGG - chr2.3:254064-254083 MS.gene86982:intron 40.0%
CTGCTTCCAGAATATGTAAC+AGG + chr2.3:252300-252319 None:intergenic 40.0%
GCAAAAGCAAGTAACCAAAG+TGG - chr2.3:252528-252547 MS.gene86982:intron 40.0%
GCAATCCTGATCAGAATTTG+AGG + chr2.3:253055-253074 None:intergenic 40.0%
GGAGGTTATGATGATTCCAT+AGG + chr2.3:254623-254642 None:intergenic 40.0%
TAACATGGTGTCTTGCAGAT+GGG - chr2.3:254032-254051 MS.gene86982:intron 40.0%
TAATCCCTCGGTTAAATACC+GGG + chr2.3:252783-252802 None:intergenic 40.0%
TACCACCTCAAATTCTGATC+AGG - chr2.3:253047-253066 MS.gene86982:intron 40.0%
TAGAAGTGGTTCTCAGTGTT+TGG - chr2.3:252560-252579 MS.gene86982:intron 40.0%
TATCTTTAACCAGTGTCTCG+GGG + chr2.3:253452-253471 None:intergenic 40.0%
TCATCCTCTAATGAGATGGA+TGG - chr2.3:252324-252343 MS.gene86982:intron 40.0%
TGTCAATTTCCTTCTCACAG+TGG + chr2.3:253140-253159 None:intergenic 40.0%
TTTACTGGACCTTGATGGAA+TGG + chr2.3:253679-253698 None:intergenic 40.0%
! AAAATACGATTTTGCCCCCT+CGG - chr2.3:254168-254187 MS.gene86982:CDS 40.0%
! TACTATTCCTGGCTTGAAGA+AGG - chr2.3:252647-252666 MS.gene86982:intron 40.0%
! TTAACTACCGAGGTTTTCCT+CGG + chr2.3:254237-254256 None:intergenic 40.0%
!! GTGATGTTGAGGATTTGCTA+GGG - chr2.3:252211-252230 MS.gene86982:intron 40.0%
!!! GCTTTTGGAACTTCAACTCT+TGG - chr2.3:253225-253244 MS.gene86982:intron 40.0%
AAGAGTCATAACCCAGTTGG+AGG - chr2.3:254683-254702 MS.gene86982:intron 45.0%
CATTCAGATCCACTGTGAGA+AGG - chr2.3:253128-253147 MS.gene86982:intron 45.0%
GCACTTTCAGCTACTATTCC+TGG - chr2.3:252636-252655 MS.gene86982:intron 45.0%
TAAATATGGGTCCCGCTAAC+CGG + chr2.3:253604-253623 None:intergenic 45.0%
TACTCGGTAGTTAACTACCG+AGG + chr2.3:254288-254307 None:intergenic 45.0%
TCATAACCCAGTTGGAGGAA+GGG - chr2.3:254688-254707 MS.gene86982:CDS 45.0%
TCTGGTGTAGTAGTAGTAGC+TGG + chr2.3:252441-252460 None:intergenic 45.0%
TGAGCCATCCATCTCATTAG+AGG + chr2.3:252331-252350 None:intergenic 45.0%
TGGAGGAATCATGGGAACAT+TGG - chr2.3:252022-252041 MS.gene86982:CDS 45.0%
TGTCACTGCTAGCAGTGTAT+TGG - chr2.3:253203-253222 MS.gene86982:intron 45.0%
TTAACTGCCGAGGAAAACCT+CGG - chr2.3:254227-254246 MS.gene86982:CDS 45.0%
TTGTCCCGGTATTTAACCGA+GGG - chr2.3:252776-252795 MS.gene86982:intron 45.0%
TTTGTCCCGGTATTTAACCG+AGG - chr2.3:252775-252794 MS.gene86982:intron 45.0%
! GGAACATTGGCCTCTTCATT+CGG - chr2.3:252035-252054 MS.gene86982:CDS 45.0%
! GGTGATGTTGAGGATTTGCT+AGG - chr2.3:252210-252229 MS.gene86982:intron 45.0%
! TGGCTTTACTATTGTCGCTC+AGG - chr2.3:253245-253264 MS.gene86982:intron 45.0%
!!! GCTAGCAGTGTATTGGCTTT+TGG - chr2.3:253210-253229 MS.gene86982:intron 45.0%
!!! TTAATGATTTTATTTTATAA+CGG - chr2.3:252836-252855 MS.gene86982:intron 5.0%
AGAACGTGTGAAGGAGCTAG+AGG - chr2.3:252957-252976 MS.gene86982:intron 50.0%
ATGGATGGCTCAGATTGCTC+AGG - chr2.3:252339-252358 MS.gene86982:intron 50.0%
CTTCTTCCCTTCCTCCAACT+GGG + chr2.3:254697-254716 None:intergenic 50.0%
GTCATAACCCAGTTGGAGGA+AGG - chr2.3:254687-254706 MS.gene86982:CDS 50.0%
GTGTCTTGCAGATGGGTGAA+GGG - chr2.3:254039-254058 MS.gene86982:intron 50.0%
TATGATTAACCAGTGCCCCG+AGG - chr2.3:253575-253594 MS.gene86982:CDS 50.0%
TGGATGGCTCAGATTGCTCA+GGG - chr2.3:252340-252359 MS.gene86982:intron 50.0%
! GGGTGAAGGGTACGAAATGA+CGG - chr2.3:254052-254071 MS.gene86982:intron 50.0%
!! TGGACGGTGTTGATGAAGGA+TGG + chr2.3:252374-252393 None:intergenic 50.0%
ACCTCGGTAGTTAACTGCCG+AGG - chr2.3:254243-254262 MS.gene86982:CDS 55.0%
CATCAACACCGTCCACTCCA+AGG - chr2.3:252379-252398 MS.gene86982:intron 55.0%
CCAGTTGGAGGAAGGGAAGA+AGG - chr2.3:254695-254714 MS.gene86982:CDS 55.0%
CCTCGGCAGTTAACTGCAGT+CGG + chr2.3:254220-254239 None:intergenic 55.0%
CCTTCTTCCCTTCCTCCAAC+TGG + chr2.3:254698-254717 None:intergenic 55.0%
CTTCGGCAGTTAACTGCCGA+GGG + chr2.3:254187-254206 None:intergenic 55.0%
GGTGTCTTGCAGATGGGTGA+AGG - chr2.3:254038-254057 MS.gene86982:intron 55.0%
TCCTCGGCAGTTAACTACCG+AGG + chr2.3:254247-254266 None:intergenic 55.0%
TCTTCGGCAGTTAACTGCCG+AGG + chr2.3:254188-254207 None:intergenic 55.0%
TTCGGCAGTTAACTGCCGAG+GGG + chr2.3:254186-254205 None:intergenic 55.0%
!! GGAGTGGACGGTGTTGATGA+AGG + chr2.3:252378-252397 None:intergenic 55.0%
AACCAGTGTCTCGGGGACAC+CGG + chr2.3:253445-253464 None:intergenic 60.0%
CCGACTGCAGTTAACTGCCG+AGG - chr2.3:254217-254236 MS.gene86982:CDS 60.0%
TCGGCAGTTAACTGCCGAGG+GGG + chr2.3:254185-254204 None:intergenic 60.0%
AACCAGTGCCCCGAGGACAC+CGG - chr2.3:253582-253601 MS.gene86982:CDS 65.0%
AACCGGTGTCCCCGAGACAC+TGG - chr2.3:253440-253459 MS.gene86982:intron 65.0%
GTCCCGCTAACCGGTGTCCT+CGG + chr2.3:253595-253614 None:intergenic 65.0%
TCCCGCTAACCGGTGTCCTC+GGG + chr2.3:253594-253613 None:intergenic 65.0%
AACCGGTGTCCTCGGGGCAC+TGG + chr2.3:253587-253606 None:intergenic 70.0%
CCCGCTAACCGGTGTCCTCG+GGG + chr2.3:253593-253612 None:intergenic 70.0%
! CCCCGAGGACACCGGTTAGC+GGG - chr2.3:253590-253609 MS.gene86982:CDS 70.0%
! GCCCCGAGGACACCGGTTAG+CGG - chr2.3:253589-253608 MS.gene86982:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 252021 254721 252021 ID=MS.gene86982
chr2.3 mRNA 252021 254721 252021 ID=MS.gene86982.t1;Parent=MS.gene86982
chr2.3 exon 254686 254721 254686 ID=MS.gene86982.t1.exon1;Parent=MS.gene86982.t1
chr2.3 CDS 254686 254721 254686 ID=cds.MS.gene86982.t1;Parent=MS.gene86982.t1
chr2.3 exon 254074 254538 254074 ID=MS.gene86982.t1.exon2;Parent=MS.gene86982.t1
chr2.3 CDS 254074 254538 254074 ID=cds.MS.gene86982.t1;Parent=MS.gene86982.t1
chr2.3 exon 253476 253838 253476 ID=MS.gene86982.t1.exon3;Parent=MS.gene86982.t1
chr2.3 CDS 253476 253838 253476 ID=cds.MS.gene86982.t1;Parent=MS.gene86982.t1
chr2.3 exon 252021 252062 252021 ID=MS.gene86982.t1.exon4;Parent=MS.gene86982.t1
chr2.3 CDS 252021 252062 252021 ID=cds.MS.gene86982.t1;Parent=MS.gene86982.t1
Gene Sequence

>MS.gene86982

ATGGAGGAATCATGGGAACATTGGCCTCTTCATTCGGTAAAAATACTAGCTATACACATCATAAATCATACTAACAAGTAATATGTTCTATGTCATTTTTTTGAGGAAGTTATTTATGTATATTGATAAATACCAGTACTATTTTCTGACATAAATAATCATTTCAATTAATGGTGACAATAGGAATTGGGTGATGTTGAGGATTTGCTAGGGCAATACTGTAAAACTAGTGCTGATGATGAAGAAGAGTTCTTGAGAGACATAATCCTTCAGCAACCTGTTACATATTCTGGAAGCAGTCTTTCATCCTCTAATGAGATGGATGGCTCAGATTGCTCAGGGAAAAAGCTCCATCCTTCATCAACACCGTCCACTCCAAGGACATTTATTCTATCTTTTGATAAATCAACCATAATACCAGCTACTACTACTACACCAGAAGAATCAGAACAAGAAGTACCAAGAACAAAGAGTAGAAGTAATAATAAGAGGAGTTTGGAACCAAAAGCAAAAGCAAGTAACCAAAGTGGAAAGAAGAGTAGAAGTGGTTCTCAGTGTTTGGATCACATTATGGCAGAGAGAAAAAGAAGACTAGAACTATCACAGAAGTTCATTGCACTTTCAGCTACTATTCCTGGCTTGAAGAAGGTAATAAAATTACAACTAAAACCCAATTTAATTAGTGCTCTTAATTTTTTTACACTAAACATTTTAGTCACTGTATTATAATAAATAAAATTAGTAGAAAGATTGATTTGTCCCGGTATTTAACCGAGGGATTAATATGTTCTTCTGTAAACATATCAGAGACTAAATTAATGATTTTATTTTATAACGGATTGAATTGGGTCGCAAGAATGATTTTGCATAAATTAATTAATAGATGGACAAGAACTATATACTTGGTGAAGCAATCAGTTATGTGAAACTACTTCAAGAACGTGTGAAGGAGCTAGAGGATCCAAACAAGAATAGCAAAGAATCAACAATAATTCTCAAGAAAACTGATATGTGTGTAAGTGAAGATACCACCTCAAATTCTGATCAGGATTGCTGCAAATCACCACTCTTTGATGTTAAAGCAAGAATATTGGAAAATGAGGTACTCATTCAGATCCACTGTGAGAAGGAAATTGACATTGAGATCAAAATATATAACGTGCTTCAAAATCTTGATCTCTTTGTCACTGCTAGCAGTGTATTGGCTTTTGGAACTTCAACTCTTGGCTTTACTATTGTCGCTCAGGTATGCATAATTTGCAAGAGTTTGATTCCATAAAACATTATGATATTATAATTGTTCAATAAATCATTCATTTGTCATCGCATAATTCAATTAGTAAAAACATTGCATAATATGTTATTTCACCTTATAAATTTATCAAACGTCTCATGAGATAATATTTAAAGGGAAATGCTAACCGGTGTCCCCGAGACACTGGTTAAAGATATGAAAAAGGAAATTATATAGTAGTTAATGCACTGAAATTGTGTAATTAATTTATAATAAAGTCAAAAACAGTTTTCTTTTATGGTAATAATTCACTTTTATGGTATGATTAACCAGTGCCCCGAGGACACCGGTTAGCGGGACCCATATTTAAATAGCTAAACATTTGAAAATAATGATCTCTCATTTGAATTATCTAAATGTTCCATTCCATCAAGGTCCAGTAAATAAAATAGTAGCCAAGTCAAACTATTCATTAATAATTATTGCTATCAACTTGATTTTGTCACATTAATTATTTTAATCAAGTTGTAGGTATTTTATCGTTCAATGCTATTAACTTAAATGTTGTGAGTTTATTCATAGATTCATCATTTTTTCGTTTTTATGATGTTAAATTTTTATTTAGTTCTAATGTACTATAAATTTGAATGAATGAGTATCGTTTCTTTTGTAAAAAATATCATATTTTATATTAATATTTTATATTAACAATAGTTCATAAATAATTTAATATATCAATATATAATTGATTAATTAAATAATCAATCTAATATTTAATAACATGGTGTCTTGCAGATGGGTGAAGGGTACGAAATGACGGTGATTGATTTGGTGAAAACTCTATCGCATGTGCTGACTAGCAGAACTGAGAAAATTGCTGTTGACACATTACATAAAGCTGTGACACACATGTAAAATACGATTTTGCCCCCTCGGCAGTTAACTGCCGAAGAATTTAAAATCCGACTGCAGTTAACTGCCGAGGAAAACCTCGGTAGTTAACTGCCGAGGAAAAACAATATTAGAAAAACCTCGGTAGTTAACTACCGAGTAAAACAGACCCTTCTTTTTTCTTCTTCTTCATCCACTTCAATCCCTTTTTTTTTTGTTTTTTTCTTCAATACTTCTAACGTAGAACATTTCCCAATTATCCAGAATCCAACTAGCAGTAAATTTGAGTATATTTCTTCAATCAAAGGCATAGAAATCATCAACAATTAATTTTAGCAATGAATTTTATCCCAATTAGAGTAATTTTCTTCAATTTTCCCAATTAGAGTAATTTTCTTCAATACACCAGTAAATTTTCTTCATGGAAAATTCAATTGAAGGCATAGAAATCATCGCTAAACATAAAAAACTCAACCTATGGAATCATCATAACCTCCAAAACTCTTCAAATAATAATTTATTTATTTTTTTCAGAAAAAGAGTCATAACCCAGTTGGAGGAAGGGAAGAAGGATGA
Protein sequence

>MS.gene86982.t1

MEESWEHWPLHSELGDVEDLLGQYCKTSADDEEEFLRDIILQQPVTYSGSSLSSSNEMDGSDCSGKKLHPSSTPSTPRTFILSFDKSTIIPATTTTPEESEQEVPRTKSRSNNKRSLEPKAKASNQSGKKSRSGSQCLDHIMAERKRRLELSQKFIALSATIPGLKKMDKNYILGEAISYVKLLQERVKELEDPNKNSKESTIILKKTDMCVSEDTTSNSDQDCCKSPLFDVKARILENEVLIQIHCEKEIDIEIKIYNVLQNLDLFVTASSVLAFGTSTLGFTIVAQKKSHNPVGGREEG