Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87018.t1 | XP_003623468.1 | 99 | 100 | 1 | 0 | 1 | 100 | 1 | 100 | 1.50E-48 | 201.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87018.t1 | G7KV21 | 99.0 | 100 | 1 | 0 | 1 | 100 | 1 | 100 | 1.1e-48 | 201.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050317 | MS.gene87018 | 0.828366 | 9.36E-55 | -1.69E-46 |
| MS.gene050320 | MS.gene87018 | 0.844089 | 9.38E-59 | -1.69E-46 |
| MS.gene051748 | MS.gene87018 | 0.817724 | 2.84E-52 | -1.69E-46 |
| MS.gene051756 | MS.gene87018 | 0.805808 | 1.12E-49 | -1.69E-46 |
| MS.gene051903 | MS.gene87018 | 0.810026 | 1.41E-50 | -1.69E-46 |
| MS.gene053109 | MS.gene87018 | 0.843887 | 1.06E-58 | -1.69E-46 |
| MS.gene057094 | MS.gene87018 | 0.802755 | 4.83E-49 | -1.69E-46 |
| MS.gene057999 | MS.gene87018 | 0.813388 | 2.63E-51 | -1.69E-46 |
| MS.gene058238 | MS.gene87018 | 0.811633 | 6.35E-51 | -1.69E-46 |
| MS.gene06011 | MS.gene87018 | 0.820142 | 8.02E-53 | -1.69E-46 |
| MS.gene060702 | MS.gene87018 | 0.815735 | 7.94E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87018.t1 | MTR_7g071210 | 99.000 | 100 | 1 | 0 | 1 | 100 | 1 | 100 | 1.66e-68 | 200 |
| MS.gene87018.t1 | MTR_3g072010 | 63.095 | 84 | 31 | 0 | 12 | 95 | 66 | 149 | 4.30e-36 | 120 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87018.t1 | AT2G38450 | 80.952 | 84 | 15 | 1 | 12 | 94 | 53 | 136 | 4.31e-46 | 145 |
| MS.gene87018.t1 | AT5G05360 | 66.667 | 90 | 30 | 0 | 6 | 95 | 71 | 160 | 1.72e-41 | 134 |
| MS.gene87018.t1 | AT5G05360 | 64.634 | 82 | 29 | 0 | 6 | 87 | 71 | 152 | 5.85e-36 | 119 |
| MS.gene87018.t1 | AT2G38450 | 76.471 | 51 | 11 | 1 | 12 | 61 | 53 | 103 | 2.07e-23 | 87.4 |
Find 22 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTGTGAAACGTTGGTTTA+AGG | 0.206492 | 7.4:+33081848 | MS.gene87018:CDS |
| TGATGTTAATATGCAGATTT+TGG | 0.257808 | 7.4:+33081896 | MS.gene87018:CDS |
| GGATATATGGAGAGCAGTAT+AGG | 0.337976 | 7.4:+33081780 | None:intergenic |
| CGAGGGTTAGGTCTTCAGTT+TGG | 0.387633 | 7.4:+33083178 | MS.gene87018:CDS |
| GATATATGGAGAGCAGTATA+GGG | 0.394996 | 7.4:+33081781 | None:intergenic |
| TGACAAAAGCATCGAGGGTT+AGG | 0.410197 | 7.4:+33083166 | MS.gene87018:CDS |
| TCTGCCACAACCCCAGATAA+AGG | 0.418971 | 7.4:-33081825 | None:intergenic |
| AGGTCTTCAGTTTGGAAAGT+TGG | 0.443829 | 7.4:+33083186 | MS.gene87018:CDS |
| GTTGGTCAGATGTATTATAG+TGG | 0.448023 | 7.4:+33081918 | MS.gene87018:CDS |
| GTATAGGGAAAGATGGCAAC+GGG | 0.500664 | 7.4:+33081796 | MS.gene87018:CDS |
| GAGAGCAGTATAGGGAAAGA+TGG | 0.541345 | 7.4:+33081789 | MS.gene87018:CDS |
| CAGATGTATTATAGTGGCTA+TGG | 0.541971 | 7.4:+33081924 | MS.gene87018:CDS |
| AAAGTTGGAGATAAGCGTCC+AGG | 0.548549 | 7.4:+33083201 | MS.gene87018:CDS |
| TGGGAATGAAGGGAAAACTT+TGG | 0.550885 | 7.4:+33083142 | MS.gene87018:intron |
| TGTTGCAAAAGATGCTCAAA+AGG | 0.554722 | 7.4:+33081947 | MS.gene87018:CDS |
| GTTTAAGGATACTCTTAGAG+AGG | 0.588468 | 7.4:+33081863 | MS.gene87018:CDS |
| ACTCTTAGAGAGGCTAAAGC+TGG | 0.589706 | 7.4:+33081873 | MS.gene87018:CDS |
| TGGCAGATTGTGTGAAACGT+TGG | 0.602973 | 7.4:+33081841 | MS.gene87018:CDS |
| TTGGCTGACAAAAGCATCGA+GGG | 0.617055 | 7.4:+33083161 | MS.gene87018:CDS |
| AGTATAGGGAAAGATGGCAA+CGG | 0.632557 | 7.4:+33081795 | MS.gene87018:CDS |
| TTTGGCTGACAAAAGCATCG+AGG | 0.704862 | 7.4:+33083160 | MS.gene87018:CDS |
| TGACTCTGATGAATCAAACG+AGG | 0.712793 | 7.4:+33083330 | MS.gene87018:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATTTTTAATGTTATGATT+TGG | - | chr7.4:33082321-33082340 | None:intergenic | 10.0% |
| !!! | TATTTTTAATGTTATGATTT+GGG | - | chr7.4:33082320-33082339 | None:intergenic | 10.0% |
| !! | ATTAAATTCATAAAAATGTG+TGG | - | chr7.4:33082795-33082814 | None:intergenic | 15.0% |
| !! | TATTTGAATATATAGAGTAA+TGG | + | chr7.4:33083025-33083044 | MS.gene87018:intron | 15.0% |
| !!! | TAACTTTTAATTTATGTTTC+TGG | + | chr7.4:33082540-33082559 | MS.gene87018:intron | 15.0% |
| !!! | TTTCTTTGTTTTTTTGTTTT+TGG | + | chr7.4:33081979-33081998 | MS.gene87018:intron | 15.0% |
| !! | AAAATAAAATAATGCTGAGT+TGG | + | chr7.4:33082377-33082396 | MS.gene87018:intron | 20.0% |
| !! | AAATAAAATAATGCTGAGTT+GGG | + | chr7.4:33082378-33082397 | MS.gene87018:intron | 20.0% |
| !! | AATATTGCATGATAATAGTT+AGG | + | chr7.4:33082104-33082123 | MS.gene87018:intron | 20.0% |
| !! | ATAATTATTTATTTCAACCG+CGG | + | chr7.4:33082590-33082609 | MS.gene87018:intron | 20.0% |
| !! | CTGATATATAAATTAAAGCA+AGG | - | chr7.4:33082974-33082993 | None:intergenic | 20.0% |
| !! | GTAGAAAGTGATAATAAATT+TGG | + | chr7.4:33082210-33082229 | MS.gene87018:intron | 20.0% |
| !! | TAAACAAATGAATAAAACCT+AGG | + | chr7.4:33082145-33082164 | MS.gene87018:intron | 20.0% |
| !!! | TGTTTTTATTTAACTTGCAT+TGG | + | chr7.4:33082016-33082035 | MS.gene87018:intron | 20.0% |
| !!! | TTTTTCTCATATTGGATTTT+AGG | + | chr7.4:33082643-33082662 | MS.gene87018:intron | 20.0% |
| ! | AAATATGAAGCAAAGTATGA+AGG | - | chr7.4:33082503-33082522 | None:intergenic | 25.0% |
| ! | AAATCCAATATGAGAAAAAG+TGG | - | chr7.4:33082642-33082661 | None:intergenic | 25.0% |
| ! | AATAAAATAATGCTGAGTTG+GGG | + | chr7.4:33082379-33082398 | MS.gene87018:intron | 25.0% |
| ! | AATATGAAGCAAAGTATGAA+GGG | - | chr7.4:33082502-33082521 | None:intergenic | 25.0% |
| ! | ATTATTTGTTTGGGAATGAA+GGG | + | chr7.4:33083132-33083151 | MS.gene87018:intron | 25.0% |
| ! | CTAATCTTCACATATAAAAC+AGG | - | chr7.4:33082832-33082851 | None:intergenic | 25.0% |
| ! | GAGGTTAACATTATTTGTTT+GGG | + | chr7.4:33083123-33083142 | MS.gene87018:intron | 25.0% |
| ! | TGTGAAGATTAGAAAAACAT+TGG | + | chr7.4:33082840-33082859 | MS.gene87018:intron | 25.0% |
| !! | CTTTCTTTTTCTTTGTAAGT+TGG | + | chr7.4:33083254-33083273 | MS.gene87018:intron | 25.0% |
| !! | TCATAAAAATGTGTGGATTT+TGG | - | chr7.4:33082788-33082807 | None:intergenic | 25.0% |
| !! | TGATGTTAATATGCAGATTT+TGG | + | chr7.4:33081896-33081915 | MS.gene87018:CDS | 25.0% |
| !!! | GCATTTTAACATCATTAAAC+TGG | - | chr7.4:33082931-33082950 | None:intergenic | 25.0% |
| !!! | TTGTTGGTAATTTTGCAAAT+CGG | + | chr7.4:33082247-33082266 | MS.gene87018:intron | 25.0% |
| !!! | TTGTTGTTTGTTTGTTTGAT+TGG | + | chr7.4:33082040-33082059 | MS.gene87018:intron | 25.0% |
| !!! | TTGTTTGCTGCATTTTTATT+AGG | + | chr7.4:33083289-33083308 | MS.gene87018:intron | 25.0% |
| AACATTGGAAGTTCTATTGT+AGG | + | chr7.4:33082855-33082874 | MS.gene87018:intron | 30.0% | |
| CATTATTTGTTTGGGAATGA+AGG | + | chr7.4:33083131-33083150 | MS.gene87018:intron | 30.0% | |
| GGAGGTTAACATTATTTGTT+TGG | + | chr7.4:33083122-33083141 | MS.gene87018:intron | 30.0% | |
| TGTTTGTTTGTTTGATTGGT+TGG | + | chr7.4:33082044-33082063 | MS.gene87018:intron | 30.0% | |
| ! | AAATGCAATGATTTGTGCTT+TGG | + | chr7.4:33082945-33082964 | MS.gene87018:intron | 30.0% |
| !! | GTTAATATGCAGATTTTGGT+TGG | + | chr7.4:33081900-33081919 | MS.gene87018:CDS | 30.0% |
| !!! | ATTGGTTGGTGTTGATTTTT+TGG | + | chr7.4:33082058-33082077 | MS.gene87018:intron | 30.0% |
| !!! | CTATCCACTTTTTCTCATAT+TGG | + | chr7.4:33082635-33082654 | MS.gene87018:intron | 30.0% |
| !!! | TTGGTTGGTGTTGATTTTTT+GGG | + | chr7.4:33082059-33082078 | MS.gene87018:intron | 30.0% |
| !!! | TTTTTCTTTGTAAGTTGGTG+TGG | + | chr7.4:33083259-33083278 | MS.gene87018:intron | 30.0% |
| ATAAGCTGCTAAAACAGTAG+AGG | - | chr7.4:33083231-33083250 | None:intergenic | 35.0% | |
| ATTGGAAGTTCTATTGTAGG+TGG | + | chr7.4:33082858-33082877 | MS.gene87018:intron | 35.0% | |
| CAGATGTATTATAGTGGCTA+TGG | + | chr7.4:33081924-33081943 | MS.gene87018:CDS | 35.0% | |
| CTATAGCGGTTATTGCATAT+CGG | + | chr7.4:33082270-33082289 | MS.gene87018:intron | 35.0% | |
| GTTGGTCAGATGTATTATAG+TGG | + | chr7.4:33081918-33081937 | MS.gene87018:CDS | 35.0% | |
| GTTTAAGGATACTCTTAGAG+AGG | + | chr7.4:33081863-33081882 | MS.gene87018:CDS | 35.0% | |
| TAAAACAGTAGAGGTTTACC+TGG | - | chr7.4:33083222-33083241 | None:intergenic | 35.0% | |
| TATGAAGTATGAACAATGCG+TGG | + | chr7.4:33083054-33083073 | MS.gene87018:intron | 35.0% | |
| TATTGATAAAACTGTCGTGG+AGG | + | chr7.4:33082691-33082710 | MS.gene87018:intron | 35.0% | |
| TGTTGCAAAAGATGCTCAAA+AGG | + | chr7.4:33081947-33081966 | MS.gene87018:CDS | 35.0% | |
| TTAATGATGAGAGGTTACCT+AGG | - | chr7.4:33082442-33082461 | None:intergenic | 35.0% | |
| ! | ATTTTGCAAATCGGCTATAG+CGG | + | chr7.4:33082256-33082275 | MS.gene87018:intron | 35.0% |
| ! | GGAAGATAGAAGTTGATTGT+TGG | + | chr7.4:33082231-33082250 | MS.gene87018:intron | 35.0% |
| ! | GTAGTTGTTTGTCTGTCTTT+TGG | + | chr7.4:33083095-33083114 | MS.gene87018:intron | 35.0% |
| !! | TGTTTGTCTGTCTTTTGGTT+TGG | + | chr7.4:33083100-33083119 | MS.gene87018:intron | 35.0% |
| !! | TTGTGTGAAACGTTGGTTTA+AGG | + | chr7.4:33081848-33081867 | MS.gene87018:CDS | 35.0% |
| !!! | GTTTGTCTGTCTTTTGGTTT+GGG | + | chr7.4:33083101-33083120 | MS.gene87018:intron | 35.0% |
| AAGATGCTCAAAAGGTGGTT+TGG | + | chr7.4:33081955-33081974 | MS.gene87018:intron | 40.0% | |
| AGAAAAAGTGGATAGCGTAG+CGG | - | chr7.4:33082630-33082649 | None:intergenic | 40.0% | |
| AGAAGCGGTTTAATGATGAG+AGG | - | chr7.4:33082451-33082470 | None:intergenic | 40.0% | |
| AGGTCTTCAGTTTGGAAAGT+TGG | + | chr7.4:33083186-33083205 | MS.gene87018:CDS | 40.0% | |
| AGTATAGGGAAAGATGGCAA+CGG | + | chr7.4:33081795-33081814 | MS.gene87018:CDS | 40.0% | |
| CGCTATTGATAAAACTGTCG+TGG | + | chr7.4:33082688-33082707 | MS.gene87018:intron | 40.0% | |
| GGGAAGAGAGAAAGATGAAA+AGG | - | chr7.4:33082482-33082501 | None:intergenic | 40.0% | |
| TAGCGAATCGCAGAAAATTG+TGG | - | chr7.4:33082673-33082692 | None:intergenic | 40.0% | |
| TATTGCTTAGATTGCAGTCG+CGG | + | chr7.4:33082732-33082751 | MS.gene87018:intron | 40.0% | |
| TCAGGTGTTCATATCTGTGT+CGG | - | chr7.4:33082176-33082195 | None:intergenic | 40.0% | |
| TGACTCTGATGAATCAAACG+AGG | + | chr7.4:33083330-33083349 | MS.gene87018:CDS | 40.0% | |
| TGCAAAAGATGCTCAAAAGG+TGG | + | chr7.4:33081950-33081969 | MS.gene87018:intron | 40.0% | |
| TGGGAATGAAGGGAAAACTT+TGG | + | chr7.4:33083142-33083161 | MS.gene87018:intron | 40.0% | |
| !! | TTTTCCTTTATCTGGGGTTG+TGG | + | chr7.4:33081821-33081840 | MS.gene87018:CDS | 40.0% |
| AAAGTTGGAGATAAGCGTCC+AGG | + | chr7.4:33083201-33083220 | MS.gene87018:CDS | 45.0% | |
| ACTCTTAGAGAGGCTAAAGC+TGG | + | chr7.4:33081873-33081892 | MS.gene87018:CDS | 45.0% | |
| ATGAGAGGTTACCTAGGTAG+TGG | - | chr7.4:33082436-33082455 | None:intergenic | 45.0% | |
| GAAAAAGTGGATAGCGTAGC+GGG | - | chr7.4:33082629-33082648 | None:intergenic | 45.0% | |
| GAAAAGGCAGAACTCAGAAG+CGG | - | chr7.4:33082466-33082485 | None:intergenic | 45.0% | |
| GAGAGCAGTATAGGGAAAGA+TGG | + | chr7.4:33081789-33081808 | MS.gene87018:CDS | 45.0% | |
| GTATAGGGAAAGATGGCAAC+GGG | + | chr7.4:33081796-33081815 | MS.gene87018:CDS | 45.0% | |
| TGAACAATGCGTGGAGTCTT+TGG | + | chr7.4:33083063-33083082 | MS.gene87018:intron | 45.0% | |
| TGAGAGGTTACCTAGGTAGT+GGG | - | chr7.4:33082435-33082454 | None:intergenic | 45.0% | |
| TGGCAGATTGTGTGAAACGT+TGG | + | chr7.4:33081841-33081860 | MS.gene87018:CDS | 45.0% | |
| ! | AACGGGCGTTTTCCTTTATC+TGG | + | chr7.4:33081813-33081832 | MS.gene87018:CDS | 45.0% |
| ! | ACGGGCGTTTTCCTTTATCT+GGG | + | chr7.4:33081814-33081833 | MS.gene87018:CDS | 45.0% |
| !! | TACCTAGGTAGTGGGGTTTA+GGG | - | chr7.4:33082427-33082446 | None:intergenic | 45.0% |
| !! | TGACAAAAGCATCGAGGGTT+AGG | + | chr7.4:33083166-33083185 | MS.gene87018:CDS | 45.0% |
| !! | TTACCTAGGTAGTGGGGTTT+AGG | - | chr7.4:33082428-33082447 | None:intergenic | 45.0% |
| !! | TTGGCTGACAAAAGCATCGA+GGG | + | chr7.4:33083161-33083180 | MS.gene87018:CDS | 45.0% |
| !! | TTTGGCTGACAAAAGCATCG+AGG | + | chr7.4:33083160-33083179 | MS.gene87018:CDS | 45.0% |
| !!! | TGTCTGTCTTTTGGTTTGGG+AGG | + | chr7.4:33083104-33083123 | MS.gene87018:intron | 45.0% |
| CGAGGGTTAGGTCTTCAGTT+TGG | + | chr7.4:33083178-33083197 | MS.gene87018:CDS | 50.0% | |
| CTACGTGTCAGTGTCATGTC+AGG | - | chr7.4:33082194-33082213 | None:intergenic | 50.0% | |
| GAGAGGTTACCTAGGTAGTG+GGG | - | chr7.4:33082434-33082453 | None:intergenic | 50.0% | |
| TCTGCCACAACCCCAGATAA+AGG | - | chr7.4:33081828-33081847 | None:intergenic | 50.0% | |
| ! | ATGCTGAGTTGGGGATGAGA+TGG | + | chr7.4:33082388-33082407 | MS.gene87018:intron | 50.0% |
| ! | CGGGCGTTTTCCTTTATCTG+GGG | + | chr7.4:33081815-33081834 | MS.gene87018:CDS | 50.0% |
| ! | TATCTGTGTCGGTGCTTCCT+AGG | - | chr7.4:33082165-33082184 | None:intergenic | 50.0% |
| ! | TGCTGAGTTGGGGATGAGAT+GGG | + | chr7.4:33082389-33082408 | MS.gene87018:intron | 50.0% |
| !! | ACCTAGGTAGTGGGGTTTAG+GGG | - | chr7.4:33082426-33082445 | None:intergenic | 50.0% |
| !! | ATAGCGTAGCGGGCAAATAG+AGG | - | chr7.4:33082619-33082638 | None:intergenic | 50.0% |
| ACCCCTAAACCCCACTACCT+AGG | + | chr7.4:33082422-33082441 | MS.gene87018:intron | 55.0% | |
| ! | GCTGAGTTGGGGATGAGATG+GGG | + | chr7.4:33082390-33082409 | MS.gene87018:intron | 55.0% |
| CGGGCAAATAGAGGAAGCCG+CGG | - | chr7.4:33082610-33082629 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 33081786 | 33083360 | 33081786 | ID=MS.gene87018 |
| chr7.4 | mRNA | 33081786 | 33083360 | 33081786 | ID=MS.gene87018.t1;Parent=MS.gene87018 |
| chr7.4 | exon | 33081786 | 33081968 | 33081786 | ID=MS.gene87018.t1.exon1;Parent=MS.gene87018.t1 |
| chr7.4 | CDS | 33081786 | 33081968 | 33081786 | ID=cds.MS.gene87018.t1;Parent=MS.gene87018.t1 |
| chr7.4 | exon | 33083153 | 33083222 | 33083153 | ID=MS.gene87018.t1.exon2;Parent=MS.gene87018.t1 |
| chr7.4 | CDS | 33083153 | 33083222 | 33083153 | ID=cds.MS.gene87018.t1;Parent=MS.gene87018.t1 |
| chr7.4 | exon | 33083311 | 33083360 | 33083311 | ID=MS.gene87018.t1.exon3;Parent=MS.gene87018.t1 |
| chr7.4 | CDS | 33083311 | 33083360 | 33083311 | ID=cds.MS.gene87018.t1;Parent=MS.gene87018.t1 |
| Gene Sequence |
| Protein sequence |
