AlfalfaGEDB | MS.gene87354

M. sativa cultivar XinJiangDaYe / MS.gene87354

KEGG: K00809
GO: GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0008612 GO:0009058 GO:0009987 GO:0018193 GO:0018205 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0051604 GO:0071704 GO:1901564
PFAM: PF01916.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87354.t1	PNX59337.1	95.7	69	3	0	1	69	9	77	3.10E-29	137.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87354.t1	Q9SC14	93.8	64	4	0	1	64	299	362	1.7e-28	125.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87354.t1	A0A2K3LI45	95.7	69	3	0	1	69	9	77	2.2e-29	137.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052519	MS.gene87354	0.812308	4.53E-51	-1.69E-46
MS.gene05289	MS.gene87354	0.800662	1.30E-48	-1.69E-46
MS.gene053166	MS.gene87354	0.814023	1.90E-51	-1.69E-46
MS.gene053967	MS.gene87354	0.817903	2.59E-52	-1.69E-46
MS.gene053998	MS.gene87354	0.801121	1.05E-48	-1.69E-46
MS.gene054559	MS.gene87354	0.805812	1.11E-49	-1.69E-46
MS.gene055818	MS.gene87354	0.822289	2.57E-53	-1.69E-46
MS.gene056498	MS.gene87354	0.80736	5.25E-50	-1.69E-46
MS.gene057060	MS.gene87354	0.803051	4.19E-49	-1.69E-46
MS.gene057444	MS.gene87354	0.822612	2.16E-53	-1.69E-46
MS.gene058979	MS.gene87354	0.80274	4.86E-49	-1.69E-46
MS.gene060322	MS.gene87354	0.841926	3.53E-58	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87354.t1	AT5G05920	92.188	64	5	1	64	108	171	6.01e-39	127
MS.gene87354.t1	AT5G05920	92.188	64	5	1	64	178	241	4.00e-38	127
MS.gene87354.t1	AT5G05920	92.188	64	5	1	64	296	359	3.81e-37	127

Find 14 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAATTTGATGGAAGTGATTC+TGG	0.236275	8.3:+25190953	MS.gene87354:CDS
AATACTGCACAAGAATTTGA+TGG	0.361085	8.3:+25190941	MS.gene87354:CDS
CCCCATGAAACAGCCTCATC+TGG	0.392601	8.3:-25190983	None:intergenic
ATCCAGATGAGGCTGTTTCA+TGG	0.429972	8.3:+25190981	MS.gene87354:CDS
TCCAGATGAGGCTGTTTCAT+GGG	0.464019	8.3:+25190982	MS.gene87354:CDS
AGGATCTGCGAAAACTGTTA+AGG	0.517573	8.3:+25191015	MS.gene87354:CDS
AACGATAGCATTCCCACTGC+TGG	0.520093	8.3:+25191154	MS.gene87354:CDS
GCGAACCTTTATCAATGAAG+AGG	0.556685	8.3:-25191206	None:intergenic
CCAGATGAGGCTGTTTCATG+GGG	0.578985	8.3:+25190983	MS.gene87354:CDS
AATGTTTCAGCAACCAGCAG+TGG	0.585353	8.3:-25191167	None:intergenic
TTCTGGAGCTCATCCAGATG+AGG	0.617682	8.3:+25190970	MS.gene87354:CDS
AACGCCAACATGATGCGTAA+TGG	0.620030	8.3:+25190896	None:intergenic
ATGTTTCAGCAACCAGCAGT+GGG	0.629148	8.3:-25191166	None:intergenic
CAGATGAGGCTGTTTCATGG+GGG	0.740121	8.3:+25190984	MS.gene87354:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATAAAAGGACATAGTTTTA+AGG	-	chr8.3:25191108-25191127	None:intergenic	20.0%
!!!	ATAAAAGGACATAGTTTTAA+GGG	-	chr8.3:25191107-25191126	None:intergenic	20.0%
!!!	TAGTTTTAAGGGATATAGTA+CGG	-	chr8.3:25191096-25191115	None:intergenic	25.0%
	AATACTGCACAAGAATTTGA+TGG	+	chr8.3:25190941-25190960	MS.gene87354:CDS	30.0%
	ACCTGGTTTAGTGAAATAAA+AGG	-	chr8.3:25191122-25191141	None:intergenic	30.0%
!	AACAAGTAGCGAAAGTATAT+AGG	-	chr8.3:25191046-25191065	None:intergenic	30.0%
!!	TCCTTTTATTTCACTAAACC+AGG	+	chr8.3:25191118-25191137	MS.gene87354:intron	30.0%
	GAATTTGATGGAAGTGATTC+TGG	+	chr8.3:25190953-25190972	MS.gene87354:CDS	35.0%
	AGGATCTGCGAAAACTGTTA+AGG	+	chr8.3:25191015-25191034	MS.gene87354:CDS	40.0%
	AATGTTTCAGCAACCAGCAG+TGG	-	chr8.3:25191170-25191189	None:intergenic	45.0%
	ATGTTTCAGCAACCAGCAGT+GGG	-	chr8.3:25191169-25191188	None:intergenic	45.0%
	GTTTCATGGGGGAAAATACG+AGG	+	chr8.3:25190995-25191014	MS.gene87354:CDS	45.0%
	TCGTTGCATCACAATGTACC+TGG	-	chr8.3:25191139-25191158	None:intergenic	45.0%
!	ATCCAGATGAGGCTGTTTCA+TGG	+	chr8.3:25190981-25191000	MS.gene87354:CDS	45.0%
!	TCCAGATGAGGCTGTTTCAT+GGG	+	chr8.3:25190982-25191001	MS.gene87354:CDS	45.0%
	AACGATAGCATTCCCACTGC+TGG	+	chr8.3:25191154-25191173	MS.gene87354:CDS	50.0%
!	CAGATGAGGCTGTTTCATGG+GGG	+	chr8.3:25190984-25191003	MS.gene87354:CDS	50.0%
!	CCAGATGAGGCTGTTTCATG+GGG	+	chr8.3:25190983-25191002	MS.gene87354:CDS	50.0%
!!	TTCTGGAGCTCATCCAGATG+AGG	+	chr8.3:25190970-25190989	MS.gene87354:CDS	50.0%
	CCCCATGAAACAGCCTCATC+TGG	-	chr8.3:25190986-25191005	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	25190905	25191217	25190905	ID=MS.gene87354
chr8.3	mRNA	25190905	25191217	25190905	ID=MS.gene87354.t1;Parent=MS.gene87354
chr8.3	exon	25190905	25191036	25190905	ID=MS.gene87354.t1.exon1;Parent=MS.gene87354.t1
chr8.3	CDS	25190905	25191036	25190905	ID=cds.MS.gene87354.t1;Parent=MS.gene87354.t1
chr8.3	exon	25191140	25191217	25191140	ID=MS.gene87354.t1.exon2;Parent=MS.gene87354.t1
chr8.3	CDS	25191140	25191217	25191140	ID=cds.MS.gene87354.t1;Parent=MS.gene87354.t1

Gene Sequence	>MS.gene87354 ATGATGCGTAATGGTGCAGACTATGCAGTTTTTATTAATACTGCACAAGAATTTGATGGAAGTGATTCTGGAGCTCATCCAGATGAGGCTGTTTCATGGGGGAAAATACGAGGATCTGCGAAAACTGTTAAGGTACATCCTATATACTTTCGCTACTTGTTTTCTTTACAGAATACACGAAACTTTTGCCGTACTATATCCCTTAAAACTATGTCCTTTTATTTCACTAAACCAGGTACATTGTGATGCAACGATAGCATTCCCACTGCTGGTTGCTGAAACATTTGCTTCAAGAATGAAACCTCTTCATTGA
Protein sequence	>MS.gene87354.t1 MMRNGADYAVFINTAQEFDGSDSGAHPDEAVSWGKIRGSAKTVKVHCDATIAFPLLVAETFASRMKPLH