Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88217.t1 | XP_024637633.1 | 97.6 | 170 | 4 | 0 | 1 | 170 | 1 | 170 | 3.60E-87 | 330.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88217.t1 | I3SF91 | 97.6 | 170 | 4 | 0 | 1 | 170 | 1 | 170 | 2.6e-87 | 330.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050128 | MS.gene88217 | 0.816088 | 6.62E-52 | -1.69E-46 |
| MS.gene050581 | MS.gene88217 | 0.830625 | 2.64E-55 | -1.69E-46 |
| MS.gene05101 | MS.gene88217 | 0.802174 | 6.36E-49 | -1.69E-46 |
| MS.gene052178 | MS.gene88217 | 0.806772 | 6.99E-50 | -1.69E-46 |
| MS.gene052287 | MS.gene88217 | 0.810073 | 1.38E-50 | -1.69E-46 |
| MS.gene054376 | MS.gene88217 | 0.804339 | 2.27E-49 | -1.69E-46 |
| MS.gene054618 | MS.gene88217 | 0.820545 | 6.48E-53 | -1.69E-46 |
| MS.gene055277 | MS.gene88217 | 0.803322 | 3.68E-49 | -1.69E-46 |
| MS.gene056191 | MS.gene88217 | 0.817625 | 2.99E-52 | -1.69E-46 |
| MS.gene056931 | MS.gene88217 | 0.825928 | 3.59E-54 | -1.69E-46 |
| MS.gene056933 | MS.gene88217 | 0.819787 | 9.67E-53 | -1.69E-46 |
| MS.gene056935 | MS.gene88217 | 0.812795 | 3.54E-51 | -1.69E-46 |
| MS.gene059228 | MS.gene88217 | 0.83216 | 1.11E-55 | -1.69E-46 |
| MS.gene059974 | MS.gene88217 | 0.835517 | 1.60E-56 | -1.69E-46 |
| MS.gene060005 | MS.gene88217 | 0.868016 | 8.93E-66 | -1.69E-46 |
| MS.gene06037 | MS.gene88217 | 0.815219 | 1.03E-51 | -1.69E-46 |
| MS.gene060559 | MS.gene88217 | 0.814006 | 1.92E-51 | -1.69E-46 |
| MS.gene060800 | MS.gene88217 | 0.80951 | 1.83E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88217.t1 | MTR_2g086680 | 67.442 | 172 | 54 | 2 | 1 | 170 | 1 | 172 | 4.88e-83 | 242 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88217.t1 | AT5G22430 | 47.134 | 157 | 81 | 2 | 15 | 170 | 17 | 172 | 7.39e-46 | 148 |
| MS.gene88217.t1 | AT2G27385 | 45.181 | 166 | 82 | 3 | 5 | 170 | 6 | 162 | 4.73e-41 | 135 |
| MS.gene88217.t1 | AT2G27385 | 45.181 | 166 | 82 | 3 | 5 | 170 | 17 | 173 | 7.29e-41 | 135 |
| MS.gene88217.t1 | AT2G27385 | 45.181 | 166 | 82 | 3 | 5 | 170 | 17 | 173 | 7.29e-41 | 135 |
Find 27 sgRNAs with CRISPR-Local
Find 91 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGCCTTACAATTTGTGTTT+TGG | 0.164797 | 4.4:-49565196 | None:intergenic |
| CAAGTTTCACAAGTTATTAA+AGG | 0.220047 | 4.4:+49565116 | MS.gene88217:CDS |
| AACTGTTTGGCAAAACTTCT+TGG | 0.296592 | 4.4:+49565059 | MS.gene88217:CDS |
| ATCAGCATGTGAAATTGTTC+AGG | 0.312927 | 4.4:+49564094 | MS.gene88217:CDS |
| CAAAGGAAGATCAATAGTTT+TGG | 0.317110 | 4.4:-49565237 | None:intergenic |
| TCTTCCTTTGCCTCCAGAAT+GGG | 0.330802 | 4.4:+49565250 | MS.gene88217:CDS |
| CTAGGTGCCAAACCCCATTC+TGG | 0.344968 | 4.4:-49565263 | None:intergenic |
| TGTAAGGCTGCAAAACCTAT+TGG | 0.353116 | 4.4:+49565209 | MS.gene88217:CDS |
| CTTTAATAACTTGTGAAACT+TGG | 0.355048 | 4.4:-49565115 | None:intergenic |
| AATTAAGGTATTCCAATGAT+AGG | 0.380107 | 4.4:-49565308 | None:intergenic |
| ACTCATAGCTACGATTTATC+TGG | 0.396323 | 4.4:+49564140 | MS.gene88217:CDS |
| TTTGCCTCCAGAATGGGGTT+TGG | 0.402516 | 4.4:+49565256 | MS.gene88217:CDS |
| ATCTTCCTTTGCCTCCAGAA+TGG | 0.404991 | 4.4:+49565249 | MS.gene88217:CDS |
| CTTCTGTAGTTGTCATAGCT+AGG | 0.461561 | 4.4:-49564983 | None:intergenic |
| GAACAATTTCACATGCTGAT+AGG | 0.462045 | 4.4:-49564091 | None:intergenic |
| TGAGGGAAGGTTAACCTTGA+AGG | 0.474424 | 4.4:-49565015 | None:intergenic |
| TGTTTGGCAAAACTTCTTGG+TGG | 0.491974 | 4.4:+49565062 | MS.gene88217:CDS |
| CTTCCTTTGCCTCCAGAATG+GGG | 0.503336 | 4.4:+49565251 | MS.gene88217:CDS |
| ATTCCAATGATAGGAACAAA+AGG | 0.504181 | 4.4:-49565299 | None:intergenic |
| AGAAGAAGATGGCTCCTTCA+AGG | 0.515472 | 4.4:+49565001 | MS.gene88217:CDS |
| CATGATATCAAGAAACTAAG+AGG | 0.542494 | 4.4:-49565170 | None:intergenic |
| AAACCCCATTCTGGAGGCAA+AGG | 0.556721 | 4.4:-49565254 | None:intergenic |
| TCAGCATGTGAAATTGTTCA+GGG | 0.561309 | 4.4:+49564095 | MS.gene88217:CDS |
| AAAGGTACATAATAGCTACT+AGG | 0.564274 | 4.4:-49565281 | None:intergenic |
| ATGACAACTACAGAAGAAGA+TGG | 0.604128 | 4.4:+49564990 | MS.gene88217:CDS |
| AAGGTCACAGTGAAATGTGA+AGG | 0.615631 | 4.4:+49564951 | MS.gene88217:CDS |
| GGTGCCAAACCCCATTCTGG+AGG | 0.664114 | 4.4:-49565260 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAGTAGACATATATATTA+TGG | + | chr4.4:49564250-49564269 | MS.gene88217:intron | 15.0% |
| !! | GAACATTTAATTAAACTAAA+AGG | - | chr4.4:49564195-49564214 | None:intergenic | 15.0% |
| !! | TTTCTTAGATATTATTAACA+AGG | - | chr4.4:49564616-49564635 | None:intergenic | 15.0% |
| !! | ATCATGTATGTTTGTTTAAA+AGG | - | chr4.4:49564443-49564462 | None:intergenic | 20.0% |
| !!! | ATGTAATTTTTTAGAAAGAG+TGG | + | chr4.4:49564739-49564758 | MS.gene88217:intron | 20.0% |
| !!! | TAAAAAATAAGAGCATTGTT+TGG | - | chr4.4:49564393-49564412 | None:intergenic | 20.0% |
| !!! | TGTAATTTTTTAGAAAGAGT+GGG | + | chr4.4:49564740-49564759 | MS.gene88217:intron | 20.0% |
| !!! | TTTTTAATGTCCCTAATTAT+GGG | + | chr4.4:49564407-49564426 | MS.gene88217:intron | 20.0% |
| !!! | TTTTTTAATGTCCCTAATTA+TGG | + | chr4.4:49564406-49564425 | MS.gene88217:intron | 20.0% |
| !!! | TTTTTTCACTCAAATCAATT+CGG | - | chr4.4:49564547-49564566 | None:intergenic | 20.0% |
| ! | ACCTTAATATCTGAAAATCA+TGG | - | chr4.4:49564936-49564955 | None:intergenic | 25.0% |
| ! | CAAGTTTCACAAGTTATTAA+AGG | + | chr4.4:49565116-49565135 | MS.gene88217:CDS | 25.0% |
| ! | TAAAATTGCTCCCATAATTA+GGG | - | chr4.4:49564420-49564439 | None:intergenic | 25.0% |
| !! | AACGTCTCAAATTATACTTT+TGG | + | chr4.4:49564712-49564731 | MS.gene88217:intron | 25.0% |
| !! | CTTTAATAACTTGTGAAACT+TGG | - | chr4.4:49565118-49565137 | None:intergenic | 25.0% |
| !! | TCCATGATTTTCAGATATTA+AGG | + | chr4.4:49564932-49564951 | MS.gene88217:intron | 25.0% |
| !!! | GTAATTTTTTAGAAAGAGTG+GGG | + | chr4.4:49564741-49564760 | MS.gene88217:intron | 25.0% |
| !!! | TAATTTTTTAGAAAGAGTGG+GGG | + | chr4.4:49564742-49564761 | MS.gene88217:intron | 25.0% |
| !!! | TGAAACTTGGTTTTGTTTTT+TGG | - | chr4.4:49565105-49565124 | None:intergenic | 25.0% |
| AAAAACTTCCATGAACTGTT+TGG | + | chr4.4:49565046-49565065 | MS.gene88217:CDS | 30.0% | |
| AAAGAAGCTATCTTGACTTA+TGG | + | chr4.4:49564768-49564787 | MS.gene88217:intron | 30.0% | |
| ATTCCAATGATAGGAACAAA+AGG | - | chr4.4:49565302-49565321 | None:intergenic | 30.0% | |
| ATTGTTTGGAGATTATAGCT+AGG | - | chr4.4:49564379-49564398 | None:intergenic | 30.0% | |
| CATGATATCAAGAAACTAAG+AGG | - | chr4.4:49565173-49565192 | None:intergenic | 30.0% | |
| GTAAAATTGCTCCCATAATT+AGG | - | chr4.4:49564421-49564440 | None:intergenic | 30.0% | |
| TTGTTTGGAGATTATAGCTA+GGG | - | chr4.4:49564378-49564397 | None:intergenic | 30.0% | |
| ! | AAAGGTACATAATAGCTACT+AGG | - | chr4.4:49565284-49565303 | None:intergenic | 30.0% |
| ! | AAGGCCCTTTATTTGATTAA+GGG | - | chr4.4:49564597-49564616 | None:intergenic | 30.0% |
| ! | AGCCTTACAATTTGTGTTTT+GGG | - | chr4.4:49565198-49565217 | None:intergenic | 30.0% |
| ! | CAAAGGAAGATCAATAGTTT+TGG | - | chr4.4:49565240-49565259 | None:intergenic | 30.0% |
| !! | ACAAGAGTTTTTGTGTTGTT+TGG | - | chr4.4:49564476-49564495 | None:intergenic | 30.0% |
| !! | TTTTTGGCATAAAGCTGAAT+TGG | - | chr4.4:49565089-49565108 | None:intergenic | 30.0% |
| !!! | AATTTTTTAGAAAGAGTGGG+GGG | + | chr4.4:49564743-49564762 | MS.gene88217:intron | 30.0% |
| !!! | ACGTTTTTTTCCTCTCTTTT+CGG | - | chr4.4:49564697-49564716 | None:intergenic | 30.0% |
| !!! | ATAGTTTTGGAAGAACCAAT+AGG | - | chr4.4:49565227-49565246 | None:intergenic | 30.0% |
| !!! | CTCCCCTAATTTTTTTGTAA+GGG | + | chr4.4:49564571-49564590 | MS.gene88217:intron | 30.0% |
| AACTGTTTGGCAAAACTTCT+TGG | + | chr4.4:49565059-49565078 | MS.gene88217:CDS | 35.0% | |
| AAGGGCCCTTAATCAAATAA+AGG | + | chr4.4:49564589-49564608 | MS.gene88217:intron | 35.0% | |
| AAGTATACTTCCGAAAAGAG+AGG | + | chr4.4:49564684-49564703 | MS.gene88217:intron | 35.0% | |
| ACTCATAGCTACGATTTATC+TGG | + | chr4.4:49564140-49564159 | MS.gene88217:CDS | 35.0% | |
| AGGGCCCTTAATCAAATAAA+GGG | + | chr4.4:49564590-49564609 | MS.gene88217:intron | 35.0% | |
| AGGGCCCTTACAAAAAAATT+AGG | - | chr4.4:49564578-49564597 | None:intergenic | 35.0% | |
| ATCAGCATGTGAAATTGTTC+AGG | + | chr4.4:49564094-49564113 | MS.gene88217:CDS | 35.0% | |
| ATGACAACTACAGAAGAAGA+TGG | + | chr4.4:49564990-49565009 | MS.gene88217:CDS | 35.0% | |
| CATCGACCATTTGACAAATA+AGG | - | chr4.4:49564820-49564839 | None:intergenic | 35.0% | |
| CCTTACAAAAAAATTAGGGG+AGG | - | chr4.4:49564573-49564592 | None:intergenic | 35.0% | |
| GAACAATTTCACATGCTGAT+AGG | - | chr4.4:49564094-49564113 | None:intergenic | 35.0% | |
| GGCCCTTACAAAAAAATTAG+GGG | - | chr4.4:49564576-49564595 | None:intergenic | 35.0% | |
| GGGCCCTTACAAAAAAATTA+GGG | - | chr4.4:49564577-49564596 | None:intergenic | 35.0% | |
| TCAATTCGGATGTACACATA+CGG | - | chr4.4:49564533-49564552 | None:intergenic | 35.0% | |
| TCAGCATGTGAAATTGTTCA+GGG | + | chr4.4:49564095-49564114 | MS.gene88217:CDS | 35.0% | |
| ! | AAGTTTTGCCAAACAGTTCA+TGG | - | chr4.4:49565057-49565076 | None:intergenic | 35.0% |
| ! | CAAGGCCCTTTATTTGATTA+AGG | - | chr4.4:49564598-49564617 | None:intergenic | 35.0% |
| ! | CAGCCTTACAATTTGTGTTT+TGG | - | chr4.4:49565199-49565218 | None:intergenic | 35.0% |
| ! | GAAGTTTTTGTGTTGTCTGA+GGG | - | chr4.4:49565035-49565054 | None:intergenic | 35.0% |
| ! | GCCTTACAATTTGTGTTTTG+GGG | - | chr4.4:49565197-49565216 | None:intergenic | 35.0% |
| ! | GTACCTTTTGTTCCTATCAT+TGG | + | chr4.4:49565296-49565315 | MS.gene88217:CDS | 35.0% |
| ! | GTTTTACCAGATGTCTCTTT+CGG | - | chr4.4:49564356-49564375 | None:intergenic | 35.0% |
| ! | TTCTAGCTAGGTACATGTTT+GGG | - | chr4.4:49564666-49564685 | None:intergenic | 35.0% |
| !! | CCTTACAATTTGTGTTTTGG+GGG | - | chr4.4:49565196-49565215 | None:intergenic | 35.0% |
| !!! | CCTCCCCTAATTTTTTTGTA+AGG | + | chr4.4:49564570-49564589 | MS.gene88217:intron | 35.0% |
| AAGGTCACAGTGAAATGTGA+AGG | + | chr4.4:49564951-49564970 | MS.gene88217:CDS | 40.0% | |
| ACTTATGGTTCCAACGATGT+TGG | + | chr4.4:49564783-49564802 | MS.gene88217:intron | 40.0% | |
| CAATTCGGATGTACACATAC+GGG | - | chr4.4:49564532-49564551 | None:intergenic | 40.0% | |
| CCCCCAAAACACAAATTGTA+AGG | + | chr4.4:49565193-49565212 | MS.gene88217:CDS | 40.0% | |
| CTTCTGTAGTTGTCATAGCT+AGG | - | chr4.4:49564986-49565005 | None:intergenic | 40.0% | |
| TGGAAGAACAATGAGAGTCA+AGG | - | chr4.4:49564916-49564935 | None:intergenic | 40.0% | |
| TGTAAGGCTGCAAAACCTAT+TGG | + | chr4.4:49565209-49565228 | MS.gene88217:CDS | 40.0% | |
| TGTTTGGCAAAACTTCTTGG+TGG | + | chr4.4:49565062-49565081 | MS.gene88217:CDS | 40.0% | |
| ! | CTTCTAGCTAGGTACATGTT+TGG | - | chr4.4:49564667-49564686 | None:intergenic | 40.0% |
| ! | GGAAGTTTTTGTGTTGTCTG+AGG | - | chr4.4:49565036-49565055 | None:intergenic | 40.0% |
| ! | TCGGAAGTATACTTCTAGCT+AGG | - | chr4.4:49564678-49564697 | None:intergenic | 40.0% |
| ! | TTTTTGTGTTGTCTGAGGGA+AGG | - | chr4.4:49565031-49565050 | None:intergenic | 40.0% |
| AGAAGAAGATGGCTCCTTCA+AGG | + | chr4.4:49565001-49565020 | MS.gene88217:CDS | 45.0% | |
| ATCTTCCTTTGCCTCCAGAA+TGG | + | chr4.4:49565249-49565268 | MS.gene88217:CDS | 45.0% | |
| GTTCCAACGATGTTGGACTT+GGG | + | chr4.4:49564790-49564809 | MS.gene88217:intron | 45.0% | |
| TACCAGATGTCTCTTTCGGT+CGG | - | chr4.4:49564352-49564371 | None:intergenic | 45.0% | |
| TCTTCCTTTGCCTCCAGAAT+GGG | + | chr4.4:49565250-49565269 | MS.gene88217:CDS | 45.0% | |
| TGAGGGAAGGTTAACCTTGA+AGG | - | chr4.4:49565018-49565037 | None:intergenic | 45.0% | |
| !! | GGTCGGCCTTATTTGTCAAA+TGG | + | chr4.4:49564811-49564830 | MS.gene88217:intron | 45.0% |
| !! | ATGTTAATTTATTTATTAAT+AGG | + | chr4.4:49564325-49564344 | MS.gene88217:intron | 5.0% |
| ACCAGATGTCTCTTTCGGTC+GGG | - | chr4.4:49564351-49564370 | None:intergenic | 50.0% | |
| CAACGATGTTGGACTTGGGT+CGG | + | chr4.4:49564794-49564813 | MS.gene88217:intron | 50.0% | |
| CTTCCTTTGCCTCCAGAATG+GGG | + | chr4.4:49565251-49565270 | MS.gene88217:CDS | 50.0% | |
| GGTTCCAACGATGTTGGACT+TGG | + | chr4.4:49564789-49564808 | MS.gene88217:intron | 50.0% | |
| TTTGCCTCCAGAATGGGGTT+TGG | + | chr4.4:49565256-49565275 | MS.gene88217:CDS | 50.0% | |
| ! | AAACCCCATTCTGGAGGCAA+AGG | - | chr4.4:49565257-49565276 | None:intergenic | 50.0% |
| CGACCCAAGTCCAACATCGT+TGG | - | chr4.4:49564796-49564815 | None:intergenic | 55.0% | |
| CTAGGTGCCAAACCCCATTC+TGG | - | chr4.4:49565266-49565285 | None:intergenic | 55.0% | |
| GCCCGACCGAAAGAGACATC+TGG | + | chr4.4:49564347-49564366 | MS.gene88217:intron | 60.0% | |
| GGTGCCAAACCCCATTCTGG+AGG | - | chr4.4:49565263-49565282 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 49564032 | 49565328 | 49564032 | ID=MS.gene88217 |
| chr4.4 | mRNA | 49564032 | 49565328 | 49564032 | ID=MS.gene88217.t1;Parent=MS.gene88217 |
| chr4.4 | exon | 49564032 | 49564161 | 49564032 | ID=MS.gene88217.t1.exon1;Parent=MS.gene88217.t1 |
| chr4.4 | CDS | 49564032 | 49564161 | 49564032 | ID=cds.MS.gene88217.t1;Parent=MS.gene88217.t1 |
| chr4.4 | exon | 49564946 | 49565328 | 49564946 | ID=MS.gene88217.t1.exon2;Parent=MS.gene88217.t1 |
| chr4.4 | CDS | 49564946 | 49565328 | 49564946 | ID=cds.MS.gene88217.t1;Parent=MS.gene88217.t1 |
| Gene Sequence |
| Protein sequence |
