Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88948.t1 | XP_013466199.1 | 95.8 | 143 | 5 | 1 | 1 | 143 | 1 | 142 | 2.50E-55 | 224.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88948.t1 | I3S747 | 95.8 | 143 | 5 | 1 | 1 | 143 | 1 | 142 | 1.8e-55 | 224.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050752 | MS.gene88948 | 0.823843 | 1.11E-53 | -1.69E-46 |
| MS.gene050838 | MS.gene88948 | 0.809143 | 2.19E-50 | -1.69E-46 |
| MS.gene050955 | MS.gene88948 | 0.807645 | 4.57E-50 | -1.69E-46 |
| MS.gene051233 | MS.gene88948 | 0.806162 | 9.41E-50 | -1.69E-46 |
| MS.gene051903 | MS.gene88948 | 0.812855 | 3.44E-51 | -1.69E-46 |
| MS.gene052335 | MS.gene88948 | 0.801094 | 1.06E-48 | -1.69E-46 |
| MS.gene052818 | MS.gene88948 | -0.801173 | 1.02E-48 | -1.69E-46 |
| MS.gene052947 | MS.gene88948 | 0.840719 | 7.34E-58 | -1.69E-46 |
| MS.gene053581 | MS.gene88948 | 0.801225 | 9.96E-49 | -1.69E-46 |
| MS.gene054403 | MS.gene88948 | 0.823972 | 1.04E-53 | -1.69E-46 |
| MS.gene054709 | MS.gene88948 | 0.809956 | 1.46E-50 | -1.69E-46 |
| MS.gene05538 | MS.gene88948 | 0.806356 | 8.56E-50 | -1.69E-46 |
| MS.gene056764 | MS.gene88948 | 0.816416 | 5.59E-52 | -1.69E-46 |
| MS.gene058074 | MS.gene88948 | 0.854407 | 1.26E-61 | -1.69E-46 |
| MS.gene059227 | MS.gene88948 | 0.800725 | 1.26E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88948.t1 | MTR_1g026078 | 93.750 | 160 | 5 | 2 | 1 | 156 | 1 | 159 | 3.66e-99 | 282 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88948.t1 | AT1G76060 | 67.785 | 149 | 42 | 3 | 4 | 148 | 8 | 154 | 9.49e-63 | 190 |
Find 34 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGACACCGTGGAAGAGCTTC+TGG | 0.320024 | 1.3:+216549 | MS.gene88948:CDS |
| ATTGTCACTCGGTTGCTTAT+CGG | 0.353596 | 1.3:+216802 | MS.gene88948:CDS |
| CCGCCACGCGCAACCGTTTG+CGG | 0.355220 | 1.3:-216509 | None:intergenic |
| ACGATGCCAAAGGCGTTCTA+TGG | 0.381120 | 1.3:+216713 | MS.gene88948:CDS |
| CAGTGCTTCTCGGCGCGTGC+TGG | 0.390621 | 1.3:-216644 | None:intergenic |
| GAGGAAAATGGCGAAGCCTT+TGG | 0.391236 | 1.3:+216464 | MS.gene88948:CDS |
| AAGGATCGTCGCGGTGGCAG+TGG | 0.428548 | 1.3:+216889 | MS.gene88948:CDS |
| AGAAGCTCTTCCACGGTGTC+AGG | 0.435099 | 1.3:-216547 | None:intergenic |
| TGTAGAGACTCAGTGCTTCT+CGG | 0.445236 | 1.3:-216654 | None:intergenic |
| AAGAAGAAGAGTTGCTGAAT+CGG | 0.460022 | 1.3:+216611 | MS.gene88948:CDS |
| ATCACGCCATAGAACGCCTT+TGG | 0.477887 | 1.3:-216719 | None:intergenic |
| TGCGAGGTTTGAGACGGATC+CGG | 0.482393 | 1.3:+216777 | MS.gene88948:CDS |
| AGCAACCGAGTGACAATCTC+CGG | 0.496816 | 1.3:-216796 | None:intergenic |
| ATAAGCTTGCGGAGAAGCAA+AGG | 0.541040 | 1.3:+216854 | MS.gene88948:CDS |
| GATCGTCGCGGTGGCAGTGG+CGG | 0.542143 | 1.3:+216892 | MS.gene88948:CDS |
| TTCACATGGCACGATGCCAA+AGG | 0.565614 | 1.3:+216703 | MS.gene88948:CDS |
| GTTTCAGATTCGAATTCCAA+AGG | 0.567422 | 1.3:-216480 | None:intergenic |
| CCGTTCCAGAAGCTCTTCCA+CGG | 0.587827 | 1.3:-216554 | None:intergenic |
| TGAGCTTGCGAGGTTTGAGA+CGG | 0.591164 | 1.3:+216771 | MS.gene88948:CDS |
| CCGCAAACGGTTGCGCGTGG+CGG | 0.594281 | 1.3:+216509 | MS.gene88948:CDS |
| TAAGCTTGCGGAGAAGCAAA+GGG | 0.598571 | 1.3:+216855 | MS.gene88948:CDS |
| GTCACTCGGTTGCTTATCGG+TGG | 0.599413 | 1.3:+216805 | MS.gene88948:CDS |
| GTAAAGAGTTTGAGCTTGCG+AGG | 0.613271 | 1.3:+216761 | MS.gene88948:CDS |
| GCAAAGGGAACAGATTCAGA+AGG | 0.622129 | 1.3:+216870 | MS.gene88948:CDS |
| CCGTGGAAGAGCTTCTGGAA+CGG | 0.622869 | 1.3:+216554 | MS.gene88948:CDS |
| AAACCGCAAACGGTTGCGCG+TGG | 0.625582 | 1.3:+216506 | MS.gene88948:CDS |
| GTTGCTTATCGGTGGACGTG+AGG | 0.631369 | 1.3:+216813 | MS.gene88948:CDS |
| CAACGCTATTGATAAGCTTG+CGG | 0.639106 | 1.3:+216843 | MS.gene88948:CDS |
| AAGAAGAGTTGCTGAATCGG+CGG | 0.650336 | 1.3:+216614 | MS.gene88948:CDS |
| ATTCAGAAGGATCGTCGCGG+TGG | 0.657849 | 1.3:+216883 | MS.gene88948:CDS |
| CGGATCCGGAGATTGTCACT+CGG | 0.686306 | 1.3:+216791 | MS.gene88948:CDS |
| ACACGACGGTCCTGACACCG+TGG | 0.698737 | 1.3:+216537 | MS.gene88948:CDS |
| GCGTGGCGGTTATTACACGA+CGG | 0.720396 | 1.3:+216523 | MS.gene88948:CDS |
| CAGATTCAGAAGGATCGTCG+CGG | 0.756987 | 1.3:+216880 | MS.gene88948:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGAAGAAGAGTTGCTGAAT+CGG | + | chr1.3:216611-216630 | MS.gene88948:CDS | 35.0% | |
| GTTTCAGATTCGAATTCCAA+AGG | - | chr1.3:216483-216502 | None:intergenic | 35.0% | |
| ATTGTCACTCGGTTGCTTAT+CGG | + | chr1.3:216802-216821 | MS.gene88948:CDS | 40.0% | |
| CAACGCTATTGATAAGCTTG+CGG | + | chr1.3:216843-216862 | MS.gene88948:CDS | 40.0% | |
| CTGAAACCAAAAACCGCAAA+CGG | + | chr1.3:216496-216515 | MS.gene88948:CDS | 40.0% | |
| ! | GTGCTTCACGATTTTTCACA+TGG | + | chr1.3:216689-216708 | MS.gene88948:CDS | 40.0% |
| AAGAAGAGTTGCTGAATCGG+CGG | + | chr1.3:216614-216633 | MS.gene88948:CDS | 45.0% | |
| ATAAGCTTGCGGAGAAGCAA+AGG | + | chr1.3:216854-216873 | MS.gene88948:CDS | 45.0% | |
| GCAAAGGGAACAGATTCAGA+AGG | + | chr1.3:216870-216889 | MS.gene88948:CDS | 45.0% | |
| GTAAAGAGTTTGAGCTTGCG+AGG | + | chr1.3:216761-216780 | MS.gene88948:CDS | 45.0% | |
| TAAGCTTGCGGAGAAGCAAA+GGG | + | chr1.3:216855-216874 | MS.gene88948:CDS | 45.0% | |
| TGTAGAGACTCAGTGCTTCT+CGG | - | chr1.3:216657-216676 | None:intergenic | 45.0% | |
| AGCAACCGAGTGACAATCTC+CGG | - | chr1.3:216799-216818 | None:intergenic | 50.0% | |
| ATCACGCCATAGAACGCCTT+TGG | - | chr1.3:216722-216741 | None:intergenic | 50.0% | |
| CAGATTCAGAAGGATCGTCG+CGG | + | chr1.3:216880-216899 | MS.gene88948:CDS | 50.0% | |
| GAGGAAAATGGCGAAGCCTT+TGG | + | chr1.3:216464-216483 | MS.gene88948:CDS | 50.0% | |
| TGAGCTTGCGAGGTTTGAGA+CGG | + | chr1.3:216771-216790 | MS.gene88948:CDS | 50.0% | |
| TTCACATGGCACGATGCCAA+AGG | + | chr1.3:216703-216722 | MS.gene88948:CDS | 50.0% | |
| !! | ACGATGCCAAAGGCGTTCTA+TGG | + | chr1.3:216713-216732 | MS.gene88948:CDS | 50.0% |
| AGAAGCTCTTCCACGGTGTC+AGG | - | chr1.3:216550-216569 | None:intergenic | 55.0% | |
| ATTCAGAAGGATCGTCGCGG+TGG | + | chr1.3:216883-216902 | MS.gene88948:CDS | 55.0% | |
| CCGTTCCAGAAGCTCTTCCA+CGG | - | chr1.3:216557-216576 | None:intergenic | 55.0% | |
| CGGATCCGGAGATTGTCACT+CGG | + | chr1.3:216791-216810 | MS.gene88948:CDS | 55.0% | |
| GCGTGGCGGTTATTACACGA+CGG | + | chr1.3:216523-216542 | MS.gene88948:CDS | 55.0% | |
| GTCACTCGGTTGCTTATCGG+TGG | + | chr1.3:216805-216824 | MS.gene88948:CDS | 55.0% | |
| GTTGCTTATCGGTGGACGTG+AGG | + | chr1.3:216813-216832 | MS.gene88948:CDS | 55.0% | |
| TGACACCGTGGAAGAGCTTC+TGG | + | chr1.3:216549-216568 | MS.gene88948:CDS | 55.0% | |
| ! | CCGTGGAAGAGCTTCTGGAA+CGG | + | chr1.3:216554-216573 | MS.gene88948:CDS | 55.0% |
| ! | GCGCAACCGTTTGCGGTTTT+TGG | - | chr1.3:216505-216524 | None:intergenic | 55.0% |
| ! | TGCGAGGTTTGAGACGGATC+CGG | + | chr1.3:216777-216796 | MS.gene88948:CDS | 55.0% |
| AAACCGCAAACGGTTGCGCG+TGG | + | chr1.3:216506-216525 | MS.gene88948:CDS | 60.0% | |
| AAGGATCGTCGCGGTGGCAG+TGG | + | chr1.3:216889-216908 | MS.gene88948:CDS | 65.0% | |
| ACACGACGGTCCTGACACCG+TGG | + | chr1.3:216537-216556 | MS.gene88948:CDS | 65.0% | |
| CAGTGCTTCTCGGCGCGTGC+TGG | - | chr1.3:216647-216666 | None:intergenic | 70.0% | |
| CCGCAAACGGTTGCGCGTGG+CGG | + | chr1.3:216509-216528 | MS.gene88948:CDS | 70.0% | |
| CCGCCACGCGCAACCGTTTG+CGG | - | chr1.3:216512-216531 | None:intergenic | 70.0% | |
| GATCGTCGCGGTGGCAGTGG+CGG | + | chr1.3:216892-216911 | MS.gene88948:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 216460 | 216930 | 216460 | ID=MS.gene88948 |
| chr1.3 | mRNA | 216460 | 216930 | 216460 | ID=MS.gene88948.t1;Parent=MS.gene88948 |
| chr1.3 | exon | 216460 | 216930 | 216460 | ID=MS.gene88948.t1.exon1;Parent=MS.gene88948.t1 |
| chr1.3 | CDS | 216460 | 216930 | 216460 | ID=cds.MS.gene88948.t1;Parent=MS.gene88948.t1 |
| Gene Sequence |
| Protein sequence |
