AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89151


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89151.t1 MTR_8g021250 100.000 95 0 0 1 95 34 128 3.60e-66 195
MS.gene89151.t1 MTR_2g042700 82.105 95 17 0 1 95 34 128 1.19e-54 166
MS.gene89151.t1 MTR_8g021250 97.222 72 2 0 1 72 34 105 5.49e-47 146
MS.gene89151.t1 MTR_2g042700 76.712 73 17 0 1 73 34 106 4.77e-38 123
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89151.t1 AT3G14080 87.368 95 12 0 1 95 34 128 2.20e-57 172
MS.gene89151.t1 AT3G14080 87.368 95 12 0 1 95 34 128 2.20e-57 172
MS.gene89151.t1 AT3G14080 87.368 95 12 0 1 95 24 118 2.63e-57 172
MS.gene89151.t1 AT1G19120 81.053 95 18 0 1 95 34 128 4.55e-52 159
MS.gene89151.t1 AT3G14080 93.750 48 3 0 1 48 34 81 2.50e-27 96.3

Find 29 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGGATGCTTCAGAACTAAA+AGG 0.302591 8.1:+78137950 MS.gene89151:CDS
GGGGAAAATGTTGTTCTAAT+TGG 0.311566 8.1:+78136344 MS.gene89151:CDS
AAGAATGGAGTTCCTTGACT+TGG 0.481713 8.1:+78137985 MS.gene89151:CDS
GCAGCTAACGCTGTTCTTGA+GGG 0.491345 8.1:+78136254 MS.gene89151:intron
GTGTTCCTACAGAAGAAATC+AGG 0.501365 8.1:+78136495 MS.gene89151:CDS
TTCTTGAGGGTGCCTGTGAG+AGG 0.503890 8.1:+78136267 MS.gene89151:CDS
GTAGGAACACGCGTCATATG+TGG 0.510896 8.1:-78136482 None:intergenic
TGCAGCTAACGCTGTTCTTG+AGG 0.511473 8.1:+78136253 MS.gene89151:intron
GATATTTCAGGCACAGAAAG+TGG 0.522168 8.1:+78137925 MS.gene89151:intron
CTATATTGTGACATCCCTCT+AGG 0.524354 8.1:+78136305 MS.gene89151:CDS
GGGATGCTTCAGAACTAAAA+GGG 0.534576 8.1:+78137951 MS.gene89151:CDS
TAGGAACACGCGTCATATGT+GGG 0.543008 8.1:-78136481 None:intergenic
TCAGGCACAGAAAGTGGAAA+GGG 0.543526 8.1:+78137931 MS.gene89151:intron
GCCTGTGAGAGGGTAATTGT+TGG 0.562739 8.1:+78136278 MS.gene89151:CDS
CTAGGTCTTTATGTAATCCG+TGG 0.570170 8.1:+78136323 MS.gene89151:CDS
TGTTGTTCTAATTGGTGAGA+TGG 0.571580 8.1:+78136352 MS.gene89151:CDS
TAGGTCTTTATGTAATCCGT+GGG 0.590765 8.1:+78136324 MS.gene89151:CDS
TTCAGGCACAGAAAGTGGAA+AGG 0.592068 8.1:+78137930 MS.gene89151:intron
TCTTGAGGGTGCCTGTGAGA+GGG 0.598880 8.1:+78136268 MS.gene89151:CDS
ACCCTCCTGATTTCTTCTGT+AGG 0.607048 8.1:-78136500 None:intergenic
ACCAACAATTACCCTCTCAC+AGG 0.608128 8.1:-78136279 None:intergenic
AGGAACACGCGTCATATGTG+GGG 0.634207 8.1:-78136480 None:intergenic
TTCCTACAGAAGAAATCAGG+AGG 0.637433 8.1:+78136498 MS.gene89151:CDS
TTAATCACTAGTCCAAGTCA+AGG 0.651795 8.1:-78137997 None:intergenic
GGATTACATAAAGACCTAGA+GGG 0.677541 8.1:-78136319 None:intergenic
GGAACACGCGTCATATGTGG+GGG 0.684221 8.1:-78136479 None:intergenic
CGGATTACATAAAGACCTAG+AGG 0.700189 8.1:-78136320 None:intergenic
TCCTACAGAAGAAATCAGGA+GGG 0.714873 8.1:+78136499 MS.gene89151:CDS
AGGTCTTTATGTAATCCGTG+GGG 0.758020 8.1:+78136325 MS.gene89151:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATTATACGACAATAAT+AGG - chr8.1:78136641-78136660 None:intergenic 15.0%
!!! ATCATAAAGAAGTTTAATTT+AGG - chr8.1:78136533-78136552 None:intergenic 15.0%
!!! TAAGATTTTATTTTTTAAGC+AGG - chr8.1:78137665-78137684 None:intergenic 15.0%
!!! TTGATATTTTTGTTATGATT+TGG + chr8.1:78137807-78137826 MS.gene89151:intron 15.0%
!! AAATAAAGTGGATAAACTAA+TGG + chr8.1:78137006-78137025 MS.gene89151:intron 20.0%
!! ATCATAACAAAAATATCAAC+TGG - chr8.1:78137806-78137825 None:intergenic 20.0%
!! GTTAAATATTCTATATTGAG+TGG + chr8.1:78137896-78137915 MS.gene89151:intron 20.0%
!! TTGAATTAATCCTAAATTTC+AGG + chr8.1:78137636-78137655 MS.gene89151:intron 20.0%
!!! AAAAGTACAATAATTGTGTT+TGG - chr8.1:78136871-78136890 None:intergenic 20.0%
!!! AGAAATAGTTTTAAATGCAA+AGG - chr8.1:78136397-78136416 None:intergenic 20.0%
!!! GAAATAGTTTTAAATGCAAA+GGG - chr8.1:78136396-78136415 None:intergenic 20.0%
!!! TGAAATTTAGGATTAATTCA+AGG - chr8.1:78137637-78137656 None:intergenic 20.0%
!!! TTCAAGGATATATCAAATTT+TGG - chr8.1:78137621-78137640 None:intergenic 20.0%
! GAGAGAATATATAGCTAATT+AGG + chr8.1:78137189-78137208 MS.gene89151:intron 25.0%
! TAACTAATTAGCTACAACTA+AGG - chr8.1:78137302-78137321 None:intergenic 25.0%
! TAGTCAGTTAGAAATTAGAT+TGG + chr8.1:78136760-78136779 MS.gene89151:intron 25.0%
! TTATGTATTACCACTGTAAA+TGG - chr8.1:78137551-78137570 None:intergenic 25.0%
! TTGGTCAATTTATACATTGA+AGG + chr8.1:78137488-78137507 MS.gene89151:intron 25.0%
!! AAATTAGATTGGTCATGTTT+GGG + chr8.1:78136771-78136790 MS.gene89151:intron 25.0%
!! TGCATACTTCATAATCATTT+TGG + chr8.1:78137029-78137048 MS.gene89151:intron 25.0%
ACATCCTGAAAATTGAAAGA+CGG - chr8.1:78136443-78136462 None:intergenic 30.0%
ACTTGAGAATATAGCTTAGT+TGG + chr8.1:78137228-78137247 MS.gene89151:intron 30.0%
CATTGTTTGTAAAGAGAAGT+AGG - chr8.1:78136674-78136693 None:intergenic 30.0%
CTACTTCTCTTTACAAACAA+TGG + chr8.1:78136672-78136691 MS.gene89151:intron 30.0%
CTTACCAATCATATGTACAA+TGG - chr8.1:78137712-78137731 None:intergenic 30.0%
GATATGTTAAAGAGGTCATT+TGG - chr8.1:78137579-78137598 None:intergenic 30.0%
GCAATGTAGATATGTTAAAG+AGG - chr8.1:78137587-78137606 None:intergenic 30.0%
TAAGCTAGTACGACTTATTA+GGG + chr8.1:78137108-78137127 MS.gene89151:intron 30.0%
TACAATGGACAAGTAGTTTA+TGG - chr8.1:78137697-78137716 None:intergenic 30.0%
TACTATATTGAAATGCTTCG+TGG + chr8.1:78137456-78137475 MS.gene89151:intron 30.0%
TATAACTCAACCATTTACAG+TGG + chr8.1:78137538-78137557 MS.gene89151:intron 30.0%
TTAAGCTAGTACGACTTATT+AGG + chr8.1:78137107-78137126 MS.gene89151:intron 30.0%
TTGTCCATTGTACATATGAT+TGG + chr8.1:78137705-78137724 MS.gene89151:intron 30.0%
! AGATGTTTTCTTTGTGTAGT+TGG + chr8.1:78137145-78137164 MS.gene89151:intron 30.0%
! GAAATTAGATTGGTCATGTT+TGG + chr8.1:78136770-78136789 MS.gene89151:intron 30.0%
AAGCAGGATTCCTGAAATTT+AGG - chr8.1:78137649-78137668 None:intergenic 35.0%
AATTAGCTACAACTAAGGAC+AGG - chr8.1:78137297-78137316 None:intergenic 35.0%
ACATTGTATTGCCTTAAGTG+AGG - chr8.1:78136590-78136609 None:intergenic 35.0%
AGGGACTATGAGAAAAAGAA+TGG + chr8.1:78137970-78137989 MS.gene89151:CDS 35.0%
CAGAGTTAGAGTTACAGAAT+TGG + chr8.1:78136800-78136819 MS.gene89151:intron 35.0%
CATTGTATTGCCTTAAGTGA+GGG - chr8.1:78136589-78136608 None:intergenic 35.0%
CTACATGAAACAGATTCGTA+GGG + chr8.1:78136729-78136748 MS.gene89151:intron 35.0%
CTAGCTTAATCGCTAGAATT+TGG - chr8.1:78137096-78137115 None:intergenic 35.0%
GAGTGGAAATGTGATATTTC+AGG + chr8.1:78137913-78137932 MS.gene89151:intron 35.0%
GGATTACATAAAGACCTAGA+GGG - chr8.1:78136322-78136341 None:intergenic 35.0%
GGGGAAAATGTTGTTCTAAT+TGG + chr8.1:78136344-78136363 MS.gene89151:CDS 35.0%
GTAAAGATTTGACATGCGTT+AGG + chr8.1:78136200-78136219 MS.gene89151:intron 35.0%
GTTAGCTGCATGTTATTGAT+AGG - chr8.1:78136243-78136262 None:intergenic 35.0%
TAGGAGGTTATCTCATAACT+CGG + chr8.1:78136829-78136848 MS.gene89151:intron 35.0%
TAGGTCTTTATGTAATCCGT+GGG + chr8.1:78136324-78136343 MS.gene89151:CDS 35.0%
TCTACATGAAACAGATTCGT+AGG + chr8.1:78136728-78136747 MS.gene89151:intron 35.0%
TGTATGACTGCATGTCAAAA+CGG - chr8.1:78137850-78137869 None:intergenic 35.0%
TGTTGTTCTAATTGGTGAGA+TGG + chr8.1:78136352-78136371 MS.gene89151:CDS 35.0%
! ACAACCGTCTTTCAATTTTC+AGG + chr8.1:78136436-78136455 MS.gene89151:intron 35.0%
! ATATGATTGGTAAGCTCTAG+TGG + chr8.1:78137718-78137737 MS.gene89151:intron 35.0%
! CTTCGCTCTTTTGATCAATT+TGG + chr8.1:78136158-78136177 MS.gene89151:CDS 35.0%
! GATATAGTGTGGGTTACTTT+AGG - chr8.1:78136914-78136933 None:intergenic 35.0%
! GTTACAGAATTGGCTTAGAT+AGG + chr8.1:78136810-78136829 MS.gene89151:intron 35.0%
! TGCTTCGTGGTTATGATTTT+TGG + chr8.1:78137469-78137488 MS.gene89151:intron 35.0%
! TTAGAACAACATTTTCCCCA+CGG - chr8.1:78136343-78136362 None:intergenic 35.0%
!! GTCTTTCAATTTTCAGGATG+TGG + chr8.1:78136442-78136461 MS.gene89151:intron 35.0%
AAGAATGGAGTTCCTTGACT+TGG + chr8.1:78137985-78138004 MS.gene89151:CDS 40.0%
AGGGATGCTTCAGAACTAAA+AGG + chr8.1:78137950-78137969 MS.gene89151:CDS 40.0%
AGGTAAAGTCGGATATAGTG+TGG - chr8.1:78136925-78136944 None:intergenic 40.0%
AGGTCTTTATGTAATCCGTG+GGG + chr8.1:78136325-78136344 MS.gene89151:CDS 40.0%
AGTACGACTTATTAGGGATG+CGG + chr8.1:78137114-78137133 MS.gene89151:intron 40.0%
ATAGGCACTTCTGCAATGAA+TGG - chr8.1:78136623-78136642 None:intergenic 40.0%
CACTATATCCGACTTTACCT+TGG + chr8.1:78136925-78136944 MS.gene89151:intron 40.0%
CGGATTACATAAAGACCTAG+AGG - chr8.1:78136323-78136342 None:intergenic 40.0%
CTAGGTCTTTATGTAATCCG+TGG + chr8.1:78136323-78136342 MS.gene89151:CDS 40.0%
CTATATTGTGACATCCCTCT+AGG + chr8.1:78136305-78136324 MS.gene89151:CDS 40.0%
GATATTTCAGGCACAGAAAG+TGG + chr8.1:78137925-78137944 MS.gene89151:intron 40.0%
GCAACGAGCTAGAAATAAAG+TGG + chr8.1:78136994-78137013 MS.gene89151:intron 40.0%
GGGATGCTTCAGAACTAAAA+GGG + chr8.1:78137951-78137970 MS.gene89151:CDS 40.0%
GGTAAAGTCGGATATAGTGT+GGG - chr8.1:78136924-78136943 None:intergenic 40.0%
GTGTTCCTACAGAAGAAATC+AGG + chr8.1:78136495-78136514 MS.gene89151:CDS 40.0%
TCCTACAGAAGAAATCAGGA+GGG + chr8.1:78136499-78136518 MS.gene89151:CDS 40.0%
TTCCTACAGAAGAAATCAGG+AGG + chr8.1:78136498-78136517 MS.gene89151:CDS 40.0%
TTTCACTCTCTGATTCACCA+GGG + chr8.1:78137394-78137413 MS.gene89151:intron 40.0%
! ACAGAATTGGCTTAGATAGG+AGG + chr8.1:78136813-78136832 MS.gene89151:intron 40.0%
!! CAATTTTCAGGATGTGGAGA+GGG + chr8.1:78136448-78136467 MS.gene89151:intron 40.0%
!! TCAATTTTCAGGATGTGGAG+AGG + chr8.1:78136447-78136466 MS.gene89151:intron 40.0%
AAACTCTTGCAGTGGATCCA+AGG - chr8.1:78136945-78136964 None:intergenic 45.0%
ACCAACAATTACCCTCTCAC+AGG - chr8.1:78136282-78136301 None:intergenic 45.0%
ACCCTCCTGATTTCTTCTGT+AGG - chr8.1:78136503-78136522 None:intergenic 45.0%
ACTGCTAAAGCAAGTATCCC+TGG - chr8.1:78137414-78137433 None:intergenic 45.0%
ACTTCTGCAATGAATGGCCA+CGG - chr8.1:78136617-78136636 None:intergenic 45.0%
CAGTGGATCCAAGGTAAAGT+CGG - chr8.1:78136936-78136955 None:intergenic 45.0%
CCAAGTTGCCTGTACTAGTA+TGG + chr8.1:78136972-78136991 MS.gene89151:intron 45.0%
CCATACTAGTACAGGCAACT+TGG - chr8.1:78136975-78136994 None:intergenic 45.0%
CTTAAGTGAGGGCAAGAACA+AGG - chr8.1:78136578-78136597 None:intergenic 45.0%
CTTGTTCTTGCCCTCACTTA+AGG + chr8.1:78136576-78136595 MS.gene89151:intron 45.0%
CTTTCACTCTCTGATTCACC+AGG + chr8.1:78137393-78137412 MS.gene89151:intron 45.0%
GTGCTGTAAAACTCTTGCAG+TGG - chr8.1:78136953-78136972 None:intergenic 45.0%
TAGGAACACGCGTCATATGT+GGG - chr8.1:78136484-78136503 None:intergenic 45.0%
TCAGGCACAGAAAGTGGAAA+GGG + chr8.1:78137931-78137950 MS.gene89151:intron 45.0%
TTCAGGCACAGAAAGTGGAA+AGG + chr8.1:78137930-78137949 MS.gene89151:intron 45.0%
AGGAACACGCGTCATATGTG+GGG - chr8.1:78136483-78136502 None:intergenic 50.0%
GCAGCTAACGCTGTTCTTGA+GGG + chr8.1:78136254-78136273 MS.gene89151:intron 50.0%
GCATGTCAAAACGGACGACA+TGG - chr8.1:78137841-78137860 None:intergenic 50.0%
GCCTGTGAGAGGGTAATTGT+TGG + chr8.1:78136278-78136297 MS.gene89151:CDS 50.0%
GCTCGTTGCCATACTAGTAC+AGG - chr8.1:78136983-78137002 None:intergenic 50.0%
GGCAATACAATGTTGTGCCG+TGG + chr8.1:78136597-78136616 MS.gene89151:intron 50.0%
GTAGGAACACGCGTCATATG+TGG - chr8.1:78136485-78136504 None:intergenic 50.0%
TGCAGCTAACGCTGTTCTTG+AGG + chr8.1:78136253-78136272 MS.gene89151:intron 50.0%
!! TTTTCAGGATGTGGAGAGGG+AGG + chr8.1:78136451-78136470 MS.gene89151:intron 50.0%
GGAACACGCGTCATATGTGG+GGG - chr8.1:78136482-78136501 None:intergenic 55.0%
!! TCTTGAGGGTGCCTGTGAGA+GGG + chr8.1:78136268-78136287 MS.gene89151:CDS 55.0%
!! TTCTTGAGGGTGCCTGTGAG+AGG + chr8.1:78136267-78136286 MS.gene89151:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 78136149 78138012 78136149 ID=MS.gene89151
chr8.1 mRNA 78136149 78138012 78136149 ID=MS.gene89151.t1;Parent=MS.gene89151
chr8.1 exon 78136149 78136179 78136149 ID=MS.gene89151.t1.exon1;Parent=MS.gene89151.t1
chr8.1 CDS 78136149 78136179 78136149 ID=cds.MS.gene89151.t1;Parent=MS.gene89151.t1
chr8.1 exon 78136258 78136373 78136258 ID=MS.gene89151.t1.exon2;Parent=MS.gene89151.t1
chr8.1 CDS 78136258 78136373 78136258 ID=cds.MS.gene89151.t1;Parent=MS.gene89151.t1
chr8.1 exon 78136458 78136520 78136458 ID=MS.gene89151.t1.exon3;Parent=MS.gene89151.t1
chr8.1 CDS 78136458 78136520 78136458 ID=cds.MS.gene89151.t1;Parent=MS.gene89151.t1
chr8.1 exon 78137935 78138012 78137935 ID=MS.gene89151.t1.exon4;Parent=MS.gene89151.t1
chr8.1 CDS 78137935 78138012 78137935 ID=cds.MS.gene89151.t1;Parent=MS.gene89151.t1
Gene Sequence

>MS.gene89151

ATGGGGACACTTCGCTCTTTTGATCAATTTGGTAACAACTTCTCAAATTACGTAAAGATTTGACATGCGTTAGGTTGAGTTTCTAATATTTCCTATCAATAACATGCAGCTAACGCTGTTCTTGAGGGTGCCTGTGAGAGGGTAATTGTTGGTGATCTATATTGTGACATCCCTCTAGGTCTTTATGTAATCCGTGGGGAAAATGTTGTTCTAATTGGTGAGATGGTATGATTGCTAACTATTTCCCTTTGCATTTAAAACTATTTCTACTTTTTAATCTCAGTTTTACAACCGTCTTTCAATTTTCAGGATGTGGAGAGGGAGGAACTTCCCCCACATATGACGCGTGTTCCTACAGAAGAAATCAGGAGGGTAAATACTCCTAAATTAAACTTCTTTATGATATTCTTTGTCTATTTTCTGTCGCCTTGTTCTTGCCCTCACTTAAGGCAATACAATGTTGTGCCGTGGCCATTCATTGCAGAAGTGCCTATTATTGTCGTATAATATATTAATATTTTTCCTACTTCTCTTTACAAACAATGGTTACATTTTGAAGTTGTAAAGCACTTCTGAACTTCTACATGAAACAGATTCGTAGGGCTTTAGAGTAGTCAGTTAGAAATTAGATTGGTCATGTTTGGGCAGTTACAGAGTTAGAGTTACAGAATTGGCTTAGATAGGAGGTTATCTCATAACTCGGATTATGTTTTTTGTTTCCAAACACAATTATTGTACTTTTGTCATTAGTTCGTCATAGAGCCTAAAGTAACCCACACTATATCCGACTTTACCTTGGATCCACTGCAAGAGTTTTACAGCACCAAGTTGCCTGTACTAGTATGGCAACGAGCTAGAAATAAAGTGGATAAACTAATGGTGCATACTTCATAATCATTTTGGATTCACTATATTAAAATTTACTTATATGTTGACTCTGCTTACCAAATTCTAGCGATTAAGCTAGTACGACTTATTAGGGATGCGGATCAGTGAAGATGTTTTCTTTGTGTAGTTGGTTGTCACATTATAAATGTATTGAGAGAATATATAGCTAATTAGGTTTCGATTTTGATACTACTTGAGAATATAGCTTAGTTGGCTTACATACATAATCTGTTATTGTAACGTCAGTAGCTCTGTTACCTGTCCTTAGTTGTAGCTAATTAGTTAGTTAATTGCTAGTTTATAAGTAGCTTTTGTACAGTTTCACAAACACAATCAATGAGAACATTTCATTTTCTCTTTCACTCTCTGATTCACCAGGGATACTTGCTTTAGCAGTAAACTAGTATTTTGCTATTGCATACTATATTGAAATGCTTCGTGGTTATGATTTTTGGTCAATTTATACATTGAAGGATTTAATATTTATGCAGAAAAGTGTCATATAACTCAACCATTTACAGTGGTAATACATAATATCACCAAATGACCTCTTTAACATATCTACATTGCAACAAGATAATCCAAAATTTGATATATCCTTGAATTAATCCTAAATTTCAGGAATCCTGCTTAAAAAATAAAATCTTAGACTAAATTCCATAAACTACTTGTCCATTGTACATATGATTGGTAAGCTCTAGTGGTTGTATTAAACTTGACAGAATGTTCTTATGATTAAAGAGCTTTGAATAAATTATTATTTTAGCCAGTTGATATTTTTGTTATGATTTGGAATTCGTTCCATGTCGTCCGTTTTGACATGCAGTCATACAAGTTGATTTTATTCAAGCAATAACTTGTTAAATATTCTATATTGAGTGGAAATGTGATATTTCAGGCACAGAAAGTGGAAAGGGATGCTTCAGAACTAAAAGGGACTATGAGAAAAAGAATGGAGTTCCTTGACTTGGACTAG

Protein sequence

>MS.gene89151.t1

MGTLRSFDQFANAVLEGACERVIVGDLYCDIPLGLYVIRGENVVLIGEMDVEREELPPHMTRVPTEEIRRAQKVERDASELKGTMRKRMEFLDLD