AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90775

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90775.t1 MTR_8g068890 98.418 316 4 1 1 316 1 315 0.0 632
MS.gene90775.t1 MTR_5g032420 81.366 322 54 4 1 316 1 322 1.14e-178 496
MS.gene90775.t1 MTR_3g100570 74.763 317 75 3 1 316 1 313 8.42e-173 481
MS.gene90775.t1 MTR_8g086070 38.941 321 150 6 3 316 18 299 1.71e-66 211
MS.gene90775.t1 MTR_7g066690 37.299 311 166 6 6 314 17 300 1.56e-61 198
MS.gene90775.t1 MTR_4g018750 36.422 313 170 6 6 316 15 300 7.80e-61 196
MS.gene90775.t1 MTR_1g090330 33.333 303 176 4 11 312 19 296 8.89e-54 178
MS.gene90775.t1 MTR_3g462140 33.013 312 183 3 4 314 13 299 2.97e-50 169
MS.gene90775.t1 MTR_4g018750 34.783 276 151 6 6 279 15 263 1.86e-44 153
MS.gene90775.t1 MTR_4g018750 28.324 173 119 3 144 311 31 203 2.29e-15 75.5
MS.gene90775.t1 MTR_8g086070 38.961 231 98 5 3 229 18 209 5.49e-43 147
MS.gene90775.t1 MTR_1g088995 31.270 307 178 5 6 312 33 306 1.00e-40 144
MS.gene90775.t1 MTR_1g088995 30.045 223 124 4 6 228 33 223 1.48e-20 89.0
MS.gene90775.t1 MTR_4g057850 25.723 311 189 9 7 309 23 299 1.88e-19 87.4
MS.gene90775.t1 MTR_4g057850 28.426 197 120 4 115 303 9 192 8.44e-13 68.2
MS.gene90775.t1 MTR_4g012850 22.684 313 201 6 6 313 16 292 2.22e-16 78.6
MS.gene90775.t1 MTR_4g012850 28.436 211 116 6 9 217 117 294 5.79e-16 77.4
MS.gene90775.t1 MTR_1g087840 24.746 295 193 7 22 313 35 303 3.20e-16 78.2
MS.gene90775.t1 MTR_8g027975 23.669 338 197 10 4 313 49 353 4.50e-16 78.2
MS.gene90775.t1 MTR_1g097890 26.990 289 185 8 33 313 34 304 7.62e-16 77.0
MS.gene90775.t1 MTR_5g009500 21.856 334 206 8 4 313 36 338 1.10e-15 77.0
MS.gene90775.t1 MTR_2g102010 27.517 298 172 12 4 294 15 275 8.14e-14 71.2
MS.gene90775.t1 MTR_2g102010 25.397 189 113 4 22 208 126 288 6.13e-11 62.8
MS.gene90775.t1 MTR_5g055410 27.063 303 176 12 22 313 37 305 9.20e-14 71.6
MS.gene90775.t1 MTR_4g088545 28.495 186 124 4 130 310 14 195 1.43e-13 69.7
MS.gene90775.t1 MTR_4g088545 28.495 186 124 4 130 310 14 195 1.88e-13 69.7
MS.gene90775.t1 MTR_3g104500 23.016 252 171 7 74 314 83 322 2.00e-13 70.1
MS.gene90775.t1 MTR_5g073040 25.159 314 191 7 11 316 40 317 2.38e-13 70.1
MS.gene90775.t1 MTR_4g119770 23.529 340 204 13 5 316 17 328 2.75e-13 69.7
MS.gene90775.t1 MTR_4g088545 28.495 186 124 4 130 310 14 195 2.78e-13 69.7
MS.gene90775.t1 MTR_4g088545 23.006 326 204 9 2 313 8 300 8.05e-12 65.5
MS.gene90775.t1 MTR_4g114320 24.490 245 168 6 76 315 164 396 9.06e-13 68.6
MS.gene90775.t1 MTR_5g030530 25.723 311 197 8 9 313 14 296 9.45e-13 67.8
MS.gene90775.t1 MTR_6g091800 25.943 212 133 6 115 311 5 207 1.32e-12 67.8
MS.gene90775.t1 MTR_7g109135 26.214 309 197 9 9 313 25 306 1.69e-12 67.4
MS.gene90775.t1 MTR_8g096050 24.923 325 178 11 6 313 227 502 2.82e-12 67.4
MS.gene90775.t1 MTR_8g076760 22.801 307 195 9 21 314 29 306 3.01e-12 66.6
MS.gene90775.t1 MTR_8g066975 26.498 317 187 12 9 313 14 296 3.97e-12 66.2
MS.gene90775.t1 MTR_8g092080 22.642 318 180 9 4 315 50 307 6.09e-12 65.9
MS.gene90775.t1 MTR_7g068340 30.337 178 105 6 144 315 103 267 8.09e-12 65.9
MS.gene90775.t1 MTR_6g034515 24.262 305 168 9 21 310 82 338 3.15e-11 63.9
MS.gene90775.t1 MTR_1g012030 23.457 243 170 6 76 313 179 410 4.82e-11 63.5
MS.gene90775.t1 MTR_7g117030 24.771 327 211 10 12 315 23 337 6.10e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90775.t1 AT4G24570 74.214 318 75 2 1 316 1 313 4.94e-169 472
MS.gene90775.t1 AT2G22500 75.316 316 73 1 1 316 1 311 1.27e-165 463
MS.gene90775.t1 AT5G09470 59.587 339 108 5 1 315 1 334 1.15e-124 360
MS.gene90775.t1 AT5G19760 39.498 319 147 5 3 314 15 294 1.57e-69 218
MS.gene90775.t1 AT5G58970 38.535 314 160 6 6 313 12 298 3.56e-65 207
MS.gene90775.t1 AT3G54110 37.898 314 168 6 4 316 13 300 1.39e-63 203
MS.gene90775.t1 AT1G14140 33.660 306 172 4 11 312 21 299 1.30e-51 172
MS.gene90775.t1 AT1G14140 26.601 203 123 2 9 211 121 297 3.88e-13 69.3
MS.gene90775.t1 AT4G03115 32.476 311 172 5 3 312 35 308 1.58e-46 159
MS.gene90775.t1 AT4G03115 32.476 311 172 5 3 312 67 340 3.22e-46 160
MS.gene90775.t1 AT5G58970 37.751 249 129 5 6 248 12 240 4.66e-44 152
MS.gene90775.t1 AT5G58970 26.590 173 122 3 144 311 32 204 3.05e-18 83.2
MS.gene90775.t1 AT4G03115 34.137 249 151 4 65 312 9 245 4.07e-42 146
MS.gene90775.t1 AT4G03115 34.583 240 144 4 74 312 10 237 9.79e-41 142
MS.gene90775.t1 AT4G03115 30.396 227 121 4 3 228 35 225 2.18e-24 99.4
MS.gene90775.t1 AT4G03115 30.396 227 121 4 3 228 35 225 3.00e-24 99.0
MS.gene90775.t1 AT5G01340 26.433 314 194 8 7 314 18 300 3.81e-22 94.7
MS.gene90775.t1 AT5G01340 27.869 183 125 3 122 303 11 187 2.13e-11 64.3
MS.gene90775.t1 AT2G33820 25.152 330 190 11 5 313 13 306 1.70e-16 79.0
MS.gene90775.t1 AT1G25380 26.821 302 183 9 22 315 37 308 2.48e-16 79.0
MS.gene90775.t1 AT1G25380 26.821 302 183 9 22 315 37 308 2.48e-16 79.0
MS.gene90775.t1 AT5G56450 25.100 251 165 7 75 314 88 326 7.84e-16 77.4
MS.gene90775.t1 AT4G01100 25.074 339 205 13 4 313 40 358 8.30e-16 77.8
MS.gene90775.t1 AT4G01100 25.000 340 190 12 4 313 40 344 1.48e-15 76.6
MS.gene90775.t1 AT5G46800 25.705 319 190 10 9 313 14 299 1.84e-15 75.9
MS.gene90775.t1 AT5G46800 22.535 213 145 3 110 313 1 202 6.19e-11 62.8
MS.gene90775.t1 AT5G46800 25.705 319 190 10 9 313 14 299 1.84e-15 75.9
MS.gene90775.t1 AT5G46800 22.535 213 145 3 110 313 1 202 6.19e-11 62.8
MS.gene90775.t1 AT5G46800 25.705 319 190 10 9 313 14 299 1.84e-15 75.9
MS.gene90775.t1 AT5G46800 22.535 213 145 3 110 313 1 202 6.19e-11 62.8
MS.gene90775.t1 AT3G53940 24.159 327 196 10 3 313 70 360 2.40e-15 76.3
MS.gene90775.t1 AT1G34065 23.567 314 182 6 4 315 73 330 3.01e-15 75.9
MS.gene90775.t1 AT1G34065 24.204 314 180 7 4 315 80 337 3.38e-15 75.5
MS.gene90775.t1 AT2G33820 24.556 338 190 12 5 313 13 314 5.60e-14 72.0
MS.gene90775.t1 AT4G01100 26.792 265 157 9 78 313 38 294 6.45e-14 71.6
MS.gene90775.t1 AT4G39460 23.871 310 182 5 4 313 56 311 2.94e-13 69.7
MS.gene90775.t1 AT4G39460 23.871 310 182 5 4 313 56 311 2.94e-13 69.7
MS.gene90775.t1 AT4G39460 23.871 310 182 5 4 313 56 311 2.94e-13 69.7
MS.gene90775.t1 AT3G53940 26.425 193 125 4 132 312 73 260 7.71e-13 68.2
MS.gene90775.t1 AT2G37890 22.118 321 208 9 4 313 43 332 1.20e-12 68.2
MS.gene90775.t1 AT2G47490 27.213 305 172 12 22 313 33 300 1.34e-12 67.8
MS.gene90775.t1 AT2G33820 23.970 267 145 9 5 243 13 249 1.75e-12 67.0
MS.gene90775.t1 AT5G66380 28.718 195 121 8 130 316 12 196 2.17e-12 67.0
MS.gene90775.t1 AT2G35800 24.841 314 185 10 3 314 542 806 2.53e-12 68.2
MS.gene90775.t1 AT4G26180 24.537 216 143 7 109 311 1 209 3.78e-12 66.2
MS.gene90775.t1 AT3G55640 25.000 196 130 5 132 315 38 228 4.21e-12 65.5
MS.gene90775.t1 AT3G55640 25.000 196 130 5 132 315 38 228 4.21e-12 65.5
MS.gene90775.t1 AT1G07030 25.767 326 185 10 6 316 36 319 4.41e-12 66.2
MS.gene90775.t1 AT3G51870 28.977 176 116 4 141 315 104 271 6.16e-12 66.2
MS.gene90775.t1 AT3G51870 24.324 296 182 10 20 313 105 360 1.08e-11 65.5
MS.gene90775.t1 AT4G26180 24.537 216 143 7 109 311 1 209 7.16e-12 65.9
MS.gene90775.t1 AT3G55640 24.747 198 136 4 126 315 36 228 7.27e-12 65.9
MS.gene90775.t1 AT3G55640 24.747 198 136 4 126 315 36 228 7.27e-12 65.9
MS.gene90775.t1 AT4G27940 24.047 341 218 10 12 314 71 408 1.94e-11 64.7
MS.gene90775.t1 AT5G17400 23.793 290 185 7 6 287 14 275 4.40e-11 63.2
MS.gene90775.t1 AT5G01500 29.121 182 110 8 140 315 131 299 4.73e-11 63.5
MS.gene90775.t1 AT5G51050 27.513 189 126 5 132 316 223 404 9.40e-11 62.8

Find 128 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCATCCTATGATGAGTTTA+AGG 0.221358 8.3:-34171158 MS.gene90775:CDS
GGGTCCTATGGCTCTTTATA+AGG 0.268391 8.3:-34170933 MS.gene90775:CDS
CGGCCCAACACGGGGCGGTT+GGG 0.300952 8.3:+34171585 None:intergenic
GGGCTGCTCGCCGGTGGGAT+TGG 0.301666 8.3:-34171379 MS.gene90775:CDS
GTGGGATTGGCGCGGCTGTC+GGG 0.356937 8.3:-34171366 MS.gene90775:CDS
CGGACTTATAGTTTCTTCTC+TGG 0.360466 8.3:+34171287 None:intergenic
TGCTAATGCTGGTCGGATAT+TGG 0.367208 8.3:+34171648 None:intergenic
CGGCCCAACCGCCCCGTGTT+GGG 0.379107 8.3:-34171588 MS.gene90775:CDS
ACAGGTTCGTAAGTTGCTTA+AGG 0.385790 8.3:-34170849 MS.gene90775:CDS
GGTGGGATTGGCGCGGCTGT+CGG 0.392305 8.3:-34171367 MS.gene90775:CDS
TGGCGCGGCTGTCGGGAATC+CGG 0.394055 8.3:-34171359 MS.gene90775:CDS
GGTCCTATGGCTCTTTATAA+GGG 0.398934 8.3:-34170932 MS.gene90775:CDS
TCGGCCCAACACGGGGCGGT+TGG 0.400715 8.3:+34171584 None:intergenic
GGCGGTTGGGCCGTCACGGC+CGG 0.409237 8.3:+34171598 None:intergenic
ACGGCCCAACCGCCCCGTGT+TGG 0.410440 8.3:-34171589 MS.gene90775:CDS
CATGGCGCGGTTCACTGTTA+AGG 0.413689 8.3:+34171201 None:intergenic
ACAAACAACACAACGGTAAA+CGG 0.423325 8.3:+34170881 None:intergenic
GCTAGTTACGGCGTCACAAC+TGG 0.426493 8.3:-34171179 MS.gene90775:CDS
GTTGTTTGTTACATTGGAAC+AGG 0.435604 8.3:-34170867 MS.gene90775:CDS
GGCATAGCTTCCATCATTGC+AGG 0.435640 8.3:-34171742 MS.gene90775:CDS
GGTTGGATGAAGGATGGGCT+TGG 0.436837 8.3:-34171118 MS.gene90775:CDS
AAGTTGCTTAAGGATTTCTA+AGG 0.437271 8.3:-34170839 None:intergenic
GGTCCCTGCCTTGTAATTGT+AGG 0.442748 8.3:+34170902 None:intergenic
ATTGGAGCGGGGCTGCTCGC+CGG 0.444367 8.3:-34171388 MS.gene90775:CDS
TTGCCGTTGACAAGTAAGAT+TGG 0.444465 8.3:-34171406 MS.gene90775:CDS
CGTTGTGTTGTTTGTTACAT+TGG 0.449852 8.3:-34170873 MS.gene90775:CDS
CTCTATTCCACCACCCGTAT+GGG 0.452624 8.3:-34171478 MS.gene90775:CDS
CAATCAATGGCGCCGGAGTA+AGG 0.454657 8.3:+34170980 None:intergenic
TCCCTCGTCTTTCGCCATCC+TGG 0.455818 8.3:+34171249 None:intergenic
GGAGCGGGGCTGCTCGCCGG+TGG 0.465110 8.3:-34171385 MS.gene90775:CDS
TGGTGTTAAGCTAGTCCAAC+AGG 0.465340 8.3:-34171554 MS.gene90775:CDS
GTTGGATGAAGGATGGGCTT+GGG 0.467370 8.3:-34171117 MS.gene90775:CDS
AACGGCGATCGGCCCAACAC+GGG 0.470119 8.3:+34171576 None:intergenic
CATTCCTACAATTACAAGGC+AGG 0.471330 8.3:-34170906 MS.gene90775:CDS
GGCGGCGGTTGCGTCGAATC+CGG 0.471512 8.3:-34171059 MS.gene90775:CDS
ACGGGGCGGTTGGGCCGTCA+CGG 0.473765 8.3:+34171594 None:intergenic
ATTAGCATTCCAACCCGGTT+CGG 0.477760 8.3:-34171632 MS.gene90775:CDS
CGGTGATCGATGAACCGAAC+CGG 0.480203 8.3:+34171618 None:intergenic
CGGTGGATGTGATTAAGACT+AGG 0.482921 8.3:-34171039 MS.gene90775:CDS
CAACGGCGATCGGCCCAACA+CGG 0.483085 8.3:+34171575 None:intergenic
TTGAAGACTGTTCGTGCTGA+GGG 0.486561 8.3:-34170953 MS.gene90775:CDS
GGACTTATAGTTTCTTCTCT+GGG 0.489330 8.3:+34171288 None:intergenic
GGAGTAAGGTGGAGGCGATC+CGG 0.492548 8.3:+34170994 None:intergenic
TGTTCGTGCTGAGGGTCCTA+TGG 0.492843 8.3:-34170945 MS.gene90775:CDS
TGTTGAACACCCTGCAATGA+TGG 0.493211 8.3:+34171732 None:intergenic
AAGGTTCGAATGCAGCTTCA+AGG 0.496054 8.3:-34171691 MS.gene90775:CDS
ATCGATGAACCGAACCGGGT+TGG 0.501708 8.3:+34171623 None:intergenic
TTGACAAGTAAGATTGGAGC+GGG 0.502593 8.3:-34171400 MS.gene90775:CDS
GCTGGTCGGATATTGGTTGT+AGG 0.505194 8.3:+34171655 None:intergenic
TCTCTATTCCACCACCCGTA+TGG 0.506394 8.3:-34171479 MS.gene90775:CDS
ATGAATATGAAGGTGGAGGC+CGG 0.509995 8.3:-34171013 MS.gene90775:CDS
AGTCTTCAAAGCACAATCAA+TGG 0.510639 8.3:+34170967 None:intergenic
CGTGTTGGGCCGATCGCCGT+TGG 0.512777 8.3:-34171574 MS.gene90775:CDS
TCATCGATCACCGGCCGTGA+CGG 0.514215 8.3:-34171608 MS.gene90775:CDS
GTGTCTCTGCCACCGTTCTC+CGG 0.514808 8.3:-34171507 MS.gene90775:CDS
AACGGCAAAGTACCGGAGAT+AGG 0.516962 8.3:+34171421 None:intergenic
AGCTTAACACCAACGGCGAT+CGG 0.517276 8.3:+34171565 None:intergenic
AAAATGGTTGGATGAAGGAT+GGG 0.517458 8.3:-34171123 MS.gene90775:CDS
TGTCGGGAATCCGGCTGATG+TGG 0.521033 8.3:-34171350 MS.gene90775:CDS
CGGCTGATGTGGCAATGGTT+AGG 0.521248 8.3:-34171339 MS.gene90775:CDS
GAACCGCGCCATGCTAGTTA+CGG 0.521850 8.3:-34171191 MS.gene90775:CDS
ATAGAGTCCCATACGGGTGG+TGG 0.523945 8.3:+34171471 None:intergenic
GAGCGGGGCTGCTCGCCGGT+GGG 0.525418 8.3:-34171384 MS.gene90775:CDS
AATAATCGCAATGGGTGTGA+AGG 0.526131 8.3:-34171779 None:intergenic
CTAACCATTGCCACATCAGC+CGG 0.528201 8.3:+34171340 None:intergenic
TAGTTTCTTCTCTGGGCCGA+TGG 0.537125 8.3:+34171295 None:intergenic
AAATGGTCTGATCCTATCTC+CGG 0.538460 8.3:-34171433 MS.gene90775:CDS
GACAGCCGCGCCAATCCCAC+CGG 0.544361 8.3:+34171369 None:intergenic
TTTGAAGACTGTTCGTGCTG+AGG 0.545035 8.3:-34170954 MS.gene90775:CDS
ACGAGGGAGTTACTAGCCTA+TGG 0.549664 8.3:-34171234 MS.gene90775:CDS
TGTGGCAATGGTTAGGATGC+AGG 0.550254 8.3:-34171332 MS.gene90775:CDS
ACTTGTCAACGGCAAAGTAC+CGG 0.558706 8.3:+34171414 None:intergenic
TTAAGGATGAACCGCGCCAT+AGG 0.560898 8.3:+34171218 None:intergenic
GGTGATCGATGAACCGAACC+GGG 0.562004 8.3:+34171619 None:intergenic
GTTTCATTCCTACAATTACA+AGG 0.563854 8.3:-34170910 MS.gene90775:CDS
GTGCTGCTACACCTTCCTGT+TGG 0.566711 8.3:+34171539 None:intergenic
CAAAGCACAATCAATGGCGC+CGG 0.568351 8.3:+34170973 None:intergenic
ATAATCGCAATGGGTGTGAA+GGG 0.570571 8.3:-34171778 None:intergenic
AAACCCTTATAAAGAGCCAT+AGG 0.570705 8.3:+34170929 None:intergenic
GAGAACGGTGGCAGAGACAC+CGG 0.571761 8.3:+34171510 None:intergenic
ATGGCGCCGGAGTAAGGTGG+AGG 0.572524 8.3:+34170986 None:intergenic
CCGGGTTGGAATGCTAATGC+TGG 0.573037 8.3:+34171637 None:intergenic
GGTGTGAAGGGTTTCGTCGA+AGG 0.574034 8.3:-34171766 MS.gene90775:CDS
CCGTCGTCGATGCTATCACC+AGG 0.578930 8.3:-34171267 MS.gene90775:CDS
GGCCGACGGAAGACTTCCAT+CGG 0.579243 8.3:-34171311 MS.gene90775:CDS
GGTGGATGTGATTAAGACTA+GGG 0.581075 8.3:-34171038 MS.gene90775:CDS
CACCAGGATGGCGAAAGACG+AGG 0.583614 8.3:-34171251 MS.gene90775:CDS
TCATATCATAGAGTCCCATA+CGG 0.584545 8.3:+34171464 None:intergenic
AACCGCCGCCACAAAACCCG+CGG 0.588953 8.3:+34171072 None:intergenic
GGTGATGAATATGAAGGTGG+AGG 0.589447 8.3:-34171017 MS.gene90775:CDS
ACGGCGATCGGCCCAACACG+GGG 0.592000 8.3:+34171577 None:intergenic
ATTCCTACAATTACAAGGCA+GGG 0.592283 8.3:-34170905 MS.gene90775:CDS
TTGTGACGCCGTAACTAGCA+TGG 0.595690 8.3:+34171183 None:intergenic
CATATCATAGAGTCCCATAC+GGG 0.597212 8.3:+34171465 None:intergenic
GACTAGGGTGATGAATATGA+AGG 0.600049 8.3:-34171023 MS.gene90775:CDS
ACCAGGATGGCGAAAGACGA+GGG 0.600800 8.3:-34171250 MS.gene90775:CDS
CTAGTCTTAATCACATCCAC+CGG 0.603477 8.3:+34171040 None:intergenic
GAATCCGGCTGATGTGGCAA+TGG 0.605065 8.3:-34171344 MS.gene90775:CDS
ACACCCTCTTGATCTCATCA+AGG 0.606884 8.3:-34171710 MS.gene90775:CDS
GTGAAGGGTTTCGTCGAAGG+AGG 0.607310 8.3:-34171763 MS.gene90775:CDS
GCTCCAATCTTACTTGTCAA+CGG 0.608573 8.3:+34171403 None:intergenic
CGAACCTTGATGAGATCAAG+AGG 0.611493 8.3:+34171706 None:intergenic
GGCCGATGGAAGTCTTCCGT+CGG 0.612610 8.3:+34171309 None:intergenic
GGCGGTTGCGTCGAATCCGG+TGG 0.613152 8.3:-34171056 MS.gene90775:CDS
ATAGAGACACTGCCGGAGAA+CGG 0.615334 8.3:+34171495 None:intergenic
ATGGTTAGGATGCAGGCCGA+CGG 0.622979 8.3:-34171325 MS.gene90775:CDS
TAGGGTGATGAATATGAAGG+TGG 0.625045 8.3:-34171020 MS.gene90775:CDS
CGGTTCGGTTCATCGATCAC+CGG 0.628932 8.3:-34171617 MS.gene90775:CDS
CCAGCATTAGCATTCCAACC+CGG 0.630776 8.3:-34171637 MS.gene90775:CDS
GCTCGCCGGTGGGATTGGCG+CGG 0.632649 8.3:-34171374 MS.gene90775:CDS
GGATCGCCTCCACCTTACTC+CGG 0.634705 8.3:-34170992 MS.gene90775:CDS
TGGTGGAATAGAGACACTGC+CGG 0.636597 8.3:+34171488 None:intergenic
TTGGACTAGCTTAACACCAA+CGG 0.639677 8.3:+34171558 None:intergenic
GTTAAGCTAGTCCAACAGGA+AGG 0.649653 8.3:-34171550 MS.gene90775:CDS
GCATAGCTTCCATCATTGCA+GGG 0.651032 8.3:-34171741 MS.gene90775:CDS
GTTGGAATGCTAATGCTGGT+CGG 0.653431 8.3:+34171641 None:intergenic
GTTGACAAGTAAGATTGGAG+CGG 0.654040 8.3:-34171401 MS.gene90775:CDS
CGTCGATGCTATCACCAGGA+TGG 0.654939 8.3:-34171263 MS.gene90775:CDS
TCAATGGCGCCGGAGTAAGG+TGG 0.657611 8.3:+34170983 None:intergenic
ATCATAGAGTCCCATACGGG+TGG 0.658486 8.3:+34171468 None:intergenic
GCGATCGGCCCAACACGGGG+CGG 0.664344 8.3:+34171580 None:intergenic
ACGCCGTAACTAGCATGGCG+CGG 0.673464 8.3:+34171188 None:intergenic
GAGACACTGCCGGAGAACGG+TGG 0.689533 8.3:+34171498 None:intergenic
CAATGTAACAAACAACACAA+CGG 0.699064 8.3:+34170874 None:intergenic
GAACCTTGATGAGATCAAGA+GGG 0.713345 8.3:+34171707 None:intergenic
GGAGTTACTAGCCTATGGCG+CGG 0.742480 8.3:-34171229 MS.gene90775:CDS
CCTGGTGATAGCATCGACGA+CGG 0.744553 8.3:+34171267 None:intergenic
TGACAAGTAAGATTGGAGCG+GGG 0.755189 8.3:-34171399 MS.gene90775:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAGATTTTAAAAAATGGT+TGG - chr8.3:34171475-34171494 MS.gene90775:CDS 15.0%
!!! AAGGAAAAGATTTTAAAAAA+TGG - chr8.3:34171471-34171490 MS.gene90775:CDS 15.0%
!! ATGATATGATGAAGAAAAAA+TGG - chr8.3:34171160-34171179 MS.gene90775:CDS 20.0%
! TTTAAAAAATGGTTGGATGA+AGG - chr8.3:34171482-34171501 MS.gene90775:CDS 25.0%
CAATGTAACAAACAACACAA+CGG + chr8.3:34171739-34171758 None:intergenic 30.0%
GTTTCATTCCTACAATTACA+AGG - chr8.3:34171700-34171719 MS.gene90775:CDS 30.0%
! CTTTTCCTTAAACTCATCAT+AGG + chr8.3:34171460-34171479 None:intergenic 30.0%
AAAAATGGTTGGATGAAGGA+TGG - chr8.3:34171486-34171505 MS.gene90775:CDS 35.0%
AAAATGGTTGGATGAAGGAT+GGG - chr8.3:34171487-34171506 MS.gene90775:CDS 35.0%
AAACCCTTATAAAGAGCCAT+AGG + chr8.3:34171684-34171703 None:intergenic 35.0%
ACAAACAACACAACGGTAAA+CGG + chr8.3:34171732-34171751 None:intergenic 35.0%
AGTCTTCAAAGCACAATCAA+TGG + chr8.3:34171646-34171665 None:intergenic 35.0%
ATTCCTACAATTACAAGGCA+GGG - chr8.3:34171705-34171724 MS.gene90775:CDS 35.0%
GGACTTATAGTTTCTTCTCT+GGG + chr8.3:34171325-34171344 None:intergenic 35.0%
TCATATCATAGAGTCCCATA+CGG + chr8.3:34171149-34171168 None:intergenic 35.0%
! GTTGTTTGTTACATTGGAAC+AGG - chr8.3:34171743-34171762 MS.gene90775:CDS 35.0%
!! CGTTGTGTTGTTTGTTACAT+TGG - chr8.3:34171737-34171756 MS.gene90775:CDS 35.0%
AAATGGTCTGATCCTATCTC+CGG - chr8.3:34171177-34171196 MS.gene90775:CDS 40.0%
ACAGGTTCGTAAGTTGCTTA+AGG - chr8.3:34171761-34171780 MS.gene90775:CDS 40.0%
CATATCATAGAGTCCCATAC+GGG + chr8.3:34171148-34171167 None:intergenic 40.0%
CATTCCTACAATTACAAGGC+AGG - chr8.3:34171704-34171723 MS.gene90775:CDS 40.0%
CGGACTTATAGTTTCTTCTC+TGG + chr8.3:34171326-34171345 None:intergenic 40.0%
GAACCTTGATGAGATCAAGA+GGG + chr8.3:34170906-34170925 None:intergenic 40.0%
GACTAGGGTGATGAATATGA+AGG - chr8.3:34171587-34171606 MS.gene90775:CDS 40.0%
GCTCCAATCTTACTTGTCAA+CGG + chr8.3:34171210-34171229 None:intergenic 40.0%
GGAAAAACTAGCTGTCACAT+GGG + chr8.3:34171520-34171539 None:intergenic 40.0%
GGCATCCTATGATGAGTTTA+AGG - chr8.3:34171452-34171471 MS.gene90775:CDS 40.0%
GGTCCTATGGCTCTTTATAA+GGG - chr8.3:34171678-34171697 MS.gene90775:CDS 40.0%
GGTGGATGTGATTAAGACTA+GGG - chr8.3:34171572-34171591 MS.gene90775:CDS 40.0%
GTTGACAAGTAAGATTGGAG+CGG - chr8.3:34171209-34171228 MS.gene90775:CDS 40.0%
TAGGGTGATGAATATGAAGG+TGG - chr8.3:34171590-34171609 MS.gene90775:CDS 40.0%
TTGACAAGTAAGATTGGAGC+GGG - chr8.3:34171210-34171229 MS.gene90775:CDS 40.0%
TTGCCGTTGACAAGTAAGAT+TGG - chr8.3:34171204-34171223 MS.gene90775:CDS 40.0%
!! CTAGTCTTAATCACATCCAC+CGG + chr8.3:34171573-34171592 None:intergenic 40.0%
!! TTGGACTAGCTTAACACCAA+CGG + chr8.3:34171055-34171074 None:intergenic 40.0%
AAGGTTCGAATGCAGCTTCA+AGG - chr8.3:34170919-34170938 MS.gene90775:CDS 45.0%
ACACCCTCTTGATCTCATCA+AGG - chr8.3:34170900-34170919 MS.gene90775:CDS 45.0%
ACTTGTCAACGGCAAAGTAC+CGG + chr8.3:34171199-34171218 None:intergenic 45.0%
ATGAATATGAAGGTGGAGGC+CGG - chr8.3:34171597-34171616 MS.gene90775:CDS 45.0%
ATTAGCATTCCAACCCGGTT+CGG - chr8.3:34170978-34170997 MS.gene90775:CDS 45.0%
CGAACCTTGATGAGATCAAG+AGG + chr8.3:34170907-34170926 None:intergenic 45.0%
CGGAAAAACTAGCTGTCACA+TGG + chr8.3:34171521-34171540 None:intergenic 45.0%
CGGTGGATGTGATTAAGACT+AGG - chr8.3:34171571-34171590 MS.gene90775:CDS 45.0%
GCATAGCTTCCATCATTGCA+GGG - chr8.3:34170869-34170888 MS.gene90775:CDS 45.0%
GGGTCCTATGGCTCTTTATA+AGG - chr8.3:34171677-34171696 MS.gene90775:CDS 45.0%
GGTGATGAATATGAAGGTGG+AGG - chr8.3:34171593-34171612 MS.gene90775:CDS 45.0%
GTTAAGCTAGTCCAACAGGA+AGG - chr8.3:34171060-34171079 MS.gene90775:CDS 45.0%
GTTGGAATGCTAATGCTGGT+CGG + chr8.3:34170972-34170991 None:intergenic 45.0%
TGACAAGTAAGATTGGAGCG+GGG - chr8.3:34171211-34171230 MS.gene90775:CDS 45.0%
TGCTAATGCTGGTCGGATAT+TGG + chr8.3:34170965-34170984 None:intergenic 45.0%
TGTTGAACACCCTGCAATGA+TGG + chr8.3:34170881-34170900 None:intergenic 45.0%
TTGAAGACTGTTCGTGCTGA+GGG - chr8.3:34171657-34171676 MS.gene90775:CDS 45.0%
TTTGAAGACTGTTCGTGCTG+AGG - chr8.3:34171656-34171675 MS.gene90775:CDS 45.0%
! TAGTTTTTCCGCGGGTTTTG+TGG - chr8.3:34171530-34171549 MS.gene90775:CDS 45.0%
! TGGTGTTAAGCTAGTCCAAC+AGG - chr8.3:34171056-34171075 MS.gene90775:CDS 45.0%
!!! GGTGTAGCAGCACTTTTTTC+CGG - chr8.3:34171081-34171100 MS.gene90775:CDS 45.0%
!!! TGTGACAGCTAGTTTTTCCG+CGG - chr8.3:34171521-34171540 MS.gene90775:CDS 45.0%
AGCTTAACACCAACGGCGAT+CGG + chr8.3:34171048-34171067 None:intergenic 50.0%
ATAGAGACACTGCCGGAGAA+CGG + chr8.3:34171118-34171137 None:intergenic 50.0%
ATCATAGAGTCCCATACGGG+TGG + chr8.3:34171145-34171164 None:intergenic 50.0%
CAAAGCACAATCAATGGCGC+CGG + chr8.3:34171640-34171659 None:intergenic 50.0%
CCAGCATTAGCATTCCAACC+CGG - chr8.3:34170973-34170992 MS.gene90775:CDS 50.0%
CTAACCATTGCCACATCAGC+CGG + chr8.3:34171273-34171292 None:intergenic 50.0%
CTCTATTCCACCACCCGTAT+GGG - chr8.3:34171132-34171151 MS.gene90775:CDS 50.0%
GGCATAGCTTCCATCATTGC+AGG - chr8.3:34170868-34170887 MS.gene90775:CDS 50.0%
GGTCCCTGCCTTGTAATTGT+AGG + chr8.3:34171711-34171730 None:intergenic 50.0%
TAGTTTCTTCTCTGGGCCGA+TGG + chr8.3:34171318-34171337 None:intergenic 50.0%
TCTCTATTCCACCACCCGTA+TGG - chr8.3:34171131-34171150 MS.gene90775:CDS 50.0%
TGGTGGAATAGAGACACTGC+CGG + chr8.3:34171125-34171144 None:intergenic 50.0%
TGTGGCAATGGTTAGGATGC+AGG - chr8.3:34171278-34171297 MS.gene90775:CDS 50.0%
TTAAGGATGAACCGCGCCAT+AGG + chr8.3:34171395-34171414 None:intergenic 50.0%
TTGTGACGCCGTAACTAGCA+TGG + chr8.3:34171430-34171449 None:intergenic 50.0%
! ACGAGGGAGTTACTAGCCTA+TGG - chr8.3:34171376-34171395 MS.gene90775:CDS 50.0%
! GCTGGTCGGATATTGGTTGT+AGG + chr8.3:34170958-34170977 None:intergenic 50.0%
! GTTGGATGAAGGATGGGCTT+GGG - chr8.3:34171493-34171512 MS.gene90775:CDS 50.0%
!! AACGGCAAAGTACCGGAGAT+AGG + chr8.3:34171192-34171211 None:intergenic 50.0%
!! TTTTTCCGCGGGTTTTGTGG+CGG - chr8.3:34171533-34171552 MS.gene90775:CDS 50.0%
!!! GTGACAGCTAGTTTTTCCGC+GGG - chr8.3:34171522-34171541 MS.gene90775:CDS 50.0%
ACCAGGATGGCGAAAGACGA+GGG - chr8.3:34171360-34171379 MS.gene90775:CDS 55.0%
ATAGAGTCCCATACGGGTGG+TGG + chr8.3:34171142-34171161 None:intergenic 55.0%
ATCGATGAACCGAACCGGGT+TGG + chr8.3:34170990-34171009 None:intergenic 55.0%
ATGGTTAGGATGCAGGCCGA+CGG - chr8.3:34171285-34171304 MS.gene90775:CDS 55.0%
CATGGCGCGGTTCACTGTTA+AGG + chr8.3:34171412-34171431 None:intergenic 55.0%
CGGCTGATGTGGCAATGGTT+AGG - chr8.3:34171271-34171290 MS.gene90775:CDS 55.0%
CGGTGATCGATGAACCGAAC+CGG + chr8.3:34170995-34171014 None:intergenic 55.0%
CGTCGATGCTATCACCAGGA+TGG - chr8.3:34171347-34171366 MS.gene90775:CDS 55.0%
GAACCGCGCCATGCTAGTTA+CGG - chr8.3:34171419-34171438 MS.gene90775:CDS 55.0%
GAATCCGGCTGATGTGGCAA+TGG - chr8.3:34171266-34171285 MS.gene90775:CDS 55.0%
GCTAGTTACGGCGTCACAAC+TGG - chr8.3:34171431-34171450 MS.gene90775:CDS 55.0%
GGTGATCGATGAACCGAACC+GGG + chr8.3:34170994-34171013 None:intergenic 55.0%
GGTGTGAAGGGTTTCGTCGA+AGG - chr8.3:34170844-34170863 MS.gene90775:CDS 55.0%
GTGAAGGGTTTCGTCGAAGG+AGG - chr8.3:34170847-34170866 MS.gene90775:CDS 55.0%
TGTTCGTGCTGAGGGTCCTA+TGG - chr8.3:34171665-34171684 MS.gene90775:CDS 55.0%
! CAATCAATGGCGCCGGAGTA+AGG + chr8.3:34171633-34171652 None:intergenic 55.0%
! CCGGGTTGGAATGCTAATGC+TGG + chr8.3:34170976-34170995 None:intergenic 55.0%
! GGAGTTACTAGCCTATGGCG+CGG - chr8.3:34171381-34171400 MS.gene90775:CDS 55.0%
! GGTTGGATGAAGGATGGGCT+TGG - chr8.3:34171492-34171511 MS.gene90775:CDS 55.0%
! GTGCTGCTACACCTTCCTGT+TGG + chr8.3:34171074-34171093 None:intergenic 55.0%
!! CCTGGTGATAGCATCGACGA+CGG + chr8.3:34171346-34171365 None:intergenic 55.0%
!! CGGTTCGGTTCATCGATCAC+CGG - chr8.3:34170993-34171012 MS.gene90775:CDS 55.0%
ACGCCGTAACTAGCATGGCG+CGG + chr8.3:34171425-34171444 None:intergenic 60.0%
CACCAGGATGGCGAAAGACG+AGG - chr8.3:34171359-34171378 MS.gene90775:CDS 60.0%
CCGTCGTCGATGCTATCACC+AGG - chr8.3:34171343-34171362 MS.gene90775:CDS 60.0%
GAGAACGGTGGCAGAGACAC+CGG + chr8.3:34171103-34171122 None:intergenic 60.0%
GGAGTAAGGTGGAGGCGATC+CGG + chr8.3:34171619-34171638 None:intergenic 60.0%
GGATCGCCTCCACCTTACTC+CGG - chr8.3:34171618-34171637 MS.gene90775:CDS 60.0%
GGCCGACGGAAGACTTCCAT+CGG - chr8.3:34171299-34171318 MS.gene90775:CDS 60.0%
GGCCGATGGAAGTCTTCCGT+CGG + chr8.3:34171304-34171323 None:intergenic 60.0%
GTGTCTCTGCCACCGTTCTC+CGG - chr8.3:34171103-34171122 MS.gene90775:CDS 60.0%
TCATCGATCACCGGCCGTGA+CGG - chr8.3:34171002-34171021 MS.gene90775:CDS 60.0%
TCCCTCGTCTTTCGCCATCC+TGG + chr8.3:34171364-34171383 None:intergenic 60.0%
TGTCGGGAATCCGGCTGATG+TGG - chr8.3:34171260-34171279 MS.gene90775:CDS 60.0%
! TCAATGGCGCCGGAGTAAGG+TGG + chr8.3:34171630-34171649 None:intergenic 60.0%
AACCGCCGCCACAAAACCCG+CGG + chr8.3:34171541-34171560 None:intergenic 65.0%
AACGGCGATCGGCCCAACAC+GGG + chr8.3:34171037-34171056 None:intergenic 65.0%
CAACGGCGATCGGCCCAACA+CGG + chr8.3:34171038-34171057 None:intergenic 65.0%
GAGACACTGCCGGAGAACGG+TGG + chr8.3:34171115-34171134 None:intergenic 65.0%
! ATGGCGCCGGAGTAAGGTGG+AGG + chr8.3:34171627-34171646 None:intergenic 65.0%
!! TTCCGCGGGTTTTGTGGCGG+CGG - chr8.3:34171536-34171555 MS.gene90775:CDS 65.0%
ACGGCGATCGGCCCAACACG+GGG + chr8.3:34171036-34171055 None:intergenic 70.0%
ATTGGAGCGGGGCTGCTCGC+CGG - chr8.3:34171222-34171241 MS.gene90775:CDS 70.0%
CGTGTTGGGCCGATCGCCGT+TGG - chr8.3:34171036-34171055 MS.gene90775:CDS 70.0%
GACAGCCGCGCCAATCCCAC+CGG + chr8.3:34171244-34171263 None:intergenic 70.0%
GGCGGCGGTTGCGTCGAATC+CGG - chr8.3:34171551-34171570 MS.gene90775:CDS 70.0%
GGCGGTTGCGTCGAATCCGG+TGG - chr8.3:34171554-34171573 MS.gene90775:CDS 70.0%
TGGCGCGGCTGTCGGGAATC+CGG - chr8.3:34171251-34171270 MS.gene90775:CDS 70.0%
! GGTGGGATTGGCGCGGCTGT+CGG - chr8.3:34171243-34171262 MS.gene90775:CDS 70.0%
! GTGGGATTGGCGCGGCTGTC+GGG - chr8.3:34171244-34171263 MS.gene90775:CDS 70.0%
ACGGCCCAACCGCCCCGTGT+TGG - chr8.3:34171021-34171040 MS.gene90775:CDS 75.0%
ACGGGGCGGTTGGGCCGTCA+CGG + chr8.3:34171019-34171038 None:intergenic 75.0%
CGGCCCAACACGGGGCGGTT+GGG + chr8.3:34171028-34171047 None:intergenic 75.0%
CGGCCCAACCGCCCCGTGTT+GGG - chr8.3:34171022-34171041 MS.gene90775:CDS 75.0%
GCGATCGGCCCAACACGGGG+CGG + chr8.3:34171033-34171052 None:intergenic 75.0%
GGGCTGCTCGCCGGTGGGAT+TGG - chr8.3:34171231-34171250 MS.gene90775:CDS 75.0%
TCGGCCCAACACGGGGCGGT+TGG + chr8.3:34171029-34171048 None:intergenic 75.0%
! GCTCGCCGGTGGGATTGGCG+CGG - chr8.3:34171236-34171255 MS.gene90775:CDS 75.0%
! GAGCGGGGCTGCTCGCCGGT+GGG - chr8.3:34171226-34171245 MS.gene90775:CDS 80.0%
! GGCGGTTGGGCCGTCACGGC+CGG + chr8.3:34171015-34171034 None:intergenic 80.0%
! GGAGCGGGGCTGCTCGCCGG+TGG - chr8.3:34171225-34171244 MS.gene90775:CDS 85.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 34170841 34171791 34170841 ID=MS.gene90775
chr8.3 mRNA 34170841 34171791 34170841 ID=MS.gene90775.t1;Parent=MS.gene90775
chr8.3 exon 34170841 34171791 34170841 ID=MS.gene90775.t1.exon1;Parent=MS.gene90775.t1
chr8.3 CDS 34170841 34171791 34170841 ID=cds.MS.gene90775.t1;Parent=MS.gene90775.t1
Gene Sequence

>MS.gene90775

ATGGGTGTGAAGGGTTTCGTCGAAGGAGGCATAGCTTCCATCATTGCAGGGTGTTCAACACACCCTCTTGATCTCATCAAGGTTCGAATGCAGCTTCAAGGAGAGAATAATACTCCTACAACCAATATCCGACCAGCATTAGCATTCCAACCCGGTTCGGTTCATCGATCACCGGCCGTGACGGCCCAACCGCCCCGTGTTGGGCCGATCGCCGTTGGTGTTAAGCTAGTCCAACAGGAAGGTGTAGCAGCACTTTTTTCCGGTGTCTCTGCCACCGTTCTCCGGCAGTGTCTCTATTCCACCACCCGTATGGGACTCTATGATATGATGAAGAAAAAATGGTCTGATCCTATCTCCGGTACTTTGCCGTTGACAAGTAAGATTGGAGCGGGGCTGCTCGCCGGTGGGATTGGCGCGGCTGTCGGGAATCCGGCTGATGTGGCAATGGTTAGGATGCAGGCCGACGGAAGACTTCCATCGGCCCAGAGAAGAAACTATAAGTCCGTCGTCGATGCTATCACCAGGATGGCGAAAGACGAGGGAGTTACTAGCCTATGGCGCGGTTCATCCTTAACAGTGAACCGCGCCATGCTAGTTACGGCGTCACAACTGGCATCCTATGATGAGTTTAAGGAAAAGATTTTAAAAAATGGTTGGATGAAGGATGGGCTTGGGACCCATGTGACAGCTAGTTTTTCCGCGGGTTTTGTGGCGGCGGTTGCGTCGAATCCGGTGGATGTGATTAAGACTAGGGTGATGAATATGAAGGTGGAGGCCGGATCGCCTCCACCTTACTCCGGCGCCATTGATTGTGCTTTGAAGACTGTTCGTGCTGAGGGTCCTATGGCTCTTTATAAGGGTTTCATTCCTACAATTACAAGGCAGGGACCGTTTACCGTTGTGTTGTTTGTTACATTGGAACAGGTTCGTAAGTTGCTTAAGGATTTCTAA
Protein sequence

>MS.gene90775.t1

MGVKGFVEGGIASIIAGCSTHPLDLIKVRMQLQGENNTPTTNIRPALAFQPGSVHRSPAVTAQPPRVGPIAVGVKLVQQEGVAALFSGVSATVLRQCLYSTTRMGLYDMMKKKWSDPISGTLPLTSKIGAGLLAGGIGAAVGNPADVAMVRMQADGRLPSAQRRNYKSVVDAITRMAKDEGVTSLWRGSSLTVNRAMLVTASQLASYDEFKEKILKNGWMKDGLGTHVTASFSAGFVAAVASNPVDVIKTRVMNMKVEAGSPPPYSGAIDCALKTVRAEGPMALYKGFIPTITRQGPFTVVLFVTLEQVRKLLKDF