Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91206.t1 | XP_013463300.1 | 99.5 | 742 | 4 | 0 | 1 | 742 | 1 | 742 | 0.00E+00 | 1469.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91206.t1 | Q94AH6 | 80.9 | 740 | 138 | 1 | 3 | 742 | 2 | 738 | 0.0e+00 | 1228.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91206.t1 | A0A072V6S2 | 99.5 | 742 | 4 | 0 | 1 | 742 | 1 | 742 | 0.0e+00 | 1469.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene96241 | MS.gene91206 | PPI |
| MS.gene34691 | MS.gene91206 | PPI |
| MS.gene91206 | MS.gene25471 | PPI |
| MS.gene35971 | MS.gene91206 | PPI |
| MS.gene91206 | MS.gene055864 | PPI |
| MS.gene91206 | MS.gene002729 | PPI |
| MS.gene004416 | MS.gene91206 | PPI |
| MS.gene25469 | MS.gene91206 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91206.t1 | MTR_2g437390 | 99.461 | 742 | 4 | 0 | 1 | 742 | 1 | 742 | 0.0 | 1526 |
| MS.gene91206.t1 | MTR_4g119413 | 93.261 | 742 | 50 | 0 | 1 | 742 | 3 | 744 | 0.0 | 1442 |
| MS.gene91206.t1 | MTR_7g108340 | 78.784 | 740 | 151 | 1 | 3 | 742 | 2 | 735 | 0.0 | 1235 |
| MS.gene91206.t1 | MTR_4g119413 | 92.808 | 584 | 42 | 0 | 1 | 584 | 3 | 586 | 0.0 | 1136 |
| MS.gene91206.t1 | MTR_8g056030 | 34.225 | 710 | 428 | 13 | 43 | 742 | 51 | 731 | 8.93e-131 | 405 |
| MS.gene91206.t1 | MTR_5g039880 | 34.153 | 732 | 439 | 15 | 23 | 742 | 32 | 732 | 1.92e-121 | 381 |
| MS.gene91206.t1 | MTR_6g011700 | 32.840 | 743 | 429 | 14 | 10 | 742 | 23 | 705 | 5.23e-120 | 376 |
| MS.gene91206.t1 | MTR_2g019260 | 33.056 | 720 | 434 | 14 | 34 | 742 | 110 | 792 | 9.39e-118 | 373 |
| MS.gene91206.t1 | MTR_6g011770 | 32.167 | 743 | 434 | 15 | 10 | 742 | 23 | 705 | 1.81e-114 | 362 |
| MS.gene91206.t1 | MTR_7g039690 | 33.243 | 740 | 413 | 18 | 32 | 742 | 21 | 708 | 9.32e-113 | 357 |
| MS.gene91206.t1 | MTR_7g039660 | 32.727 | 660 | 401 | 11 | 92 | 742 | 95 | 720 | 1.94e-106 | 341 |
| MS.gene91206.t1 | MTR_2g019260 | 31.633 | 686 | 423 | 13 | 34 | 708 | 110 | 760 | 2.41e-105 | 339 |
| MS.gene91206.t1 | MTR_1g040925 | 33.828 | 606 | 357 | 10 | 144 | 742 | 373 | 941 | 3.75e-92 | 308 |
| MS.gene91206.t1 | MTR_1g040925 | 26.545 | 275 | 182 | 6 | 92 | 363 | 102 | 359 | 1.29e-26 | 117 |
| MS.gene91206.t1 | MTR_5g039770 | 31.287 | 668 | 396 | 13 | 89 | 742 | 36 | 654 | 4.15e-87 | 288 |
| MS.gene91206.t1 | MTR_7g039790 | 30.120 | 747 | 450 | 18 | 9 | 742 | 30 | 717 | 3.06e-85 | 285 |
| MS.gene91206.t1 | MTR_1g040995 | 33.138 | 513 | 320 | 8 | 144 | 654 | 373 | 864 | 3.15e-78 | 269 |
| MS.gene91206.t1 | MTR_1g040995 | 26.545 | 275 | 182 | 6 | 92 | 363 | 102 | 359 | 1.17e-26 | 117 |
| MS.gene91206.t1 | MTR_7g445240 | 29.278 | 748 | 439 | 20 | 9 | 742 | 30 | 701 | 2.89e-76 | 260 |
| MS.gene91206.t1 | MTR_4g119490 | 55.500 | 200 | 89 | 0 | 7 | 206 | 5 | 204 | 1.83e-71 | 232 |
| MS.gene91206.t1 | MTR_4g119450 | 54.902 | 204 | 88 | 2 | 5 | 206 | 3 | 204 | 2.23e-68 | 224 |
| MS.gene91206.t1 | MTR_4g119422 | 52.941 | 221 | 96 | 3 | 7 | 222 | 3 | 220 | 7.09e-65 | 220 |
| MS.gene91206.t1 | MTR_4g119930 | 53.171 | 205 | 93 | 1 | 2 | 206 | 4 | 205 | 1.49e-62 | 208 |
| MS.gene91206.t1 | MTR_4g119930 | 58.333 | 156 | 62 | 1 | 2 | 157 | 4 | 156 | 8.49e-51 | 175 |
| MS.gene91206.t1 | MTR_5g040040 | 34.675 | 323 | 172 | 6 | 427 | 742 | 296 | 586 | 3.22e-46 | 174 |
| MS.gene91206.t1 | MTR_4g119930 | 50.955 | 157 | 77 | 0 | 50 | 206 | 1 | 157 | 5.83e-43 | 153 |
| MS.gene91206.t1 | MTR_5g039890 | 26.712 | 438 | 275 | 11 | 43 | 446 | 49 | 474 | 7.88e-40 | 154 |
| MS.gene91206.t1 | MTR_1g040965 | 41.284 | 218 | 121 | 3 | 532 | 742 | 1 | 218 | 3.61e-38 | 142 |
| MS.gene91206.t1 | MTR_3g115680 | 38.043 | 184 | 102 | 3 | 2 | 182 | 4 | 178 | 6.30e-34 | 129 |
| MS.gene91206.t1 | MTR_5g040180 | 27.036 | 307 | 205 | 5 | 76 | 380 | 69 | 358 | 5.31e-32 | 128 |
| MS.gene91206.t1 | MTR_7g039440 | 26.710 | 307 | 206 | 6 | 76 | 380 | 5 | 294 | 5.62e-32 | 127 |
| MS.gene91206.t1 | MTR_7g039680 | 26.377 | 345 | 215 | 8 | 43 | 385 | 49 | 356 | 1.93e-31 | 126 |
| MS.gene91206.t1 | MTR_7g039430 | 28.516 | 256 | 174 | 5 | 76 | 329 | 69 | 317 | 9.27e-28 | 115 |
| MS.gene91206.t1 | MTR_5g040190 | 24.277 | 346 | 243 | 7 | 9 | 352 | 12 | 340 | 1.18e-27 | 115 |
| MS.gene91206.t1 | MTR_4g131910 | 55.422 | 83 | 36 | 1 | 1 | 83 | 1 | 82 | 1.88e-21 | 94.7 |
| MS.gene91206.t1 | MTR_7g039770 | 27.715 | 267 | 120 | 7 | 478 | 742 | 1 | 196 | 9.63e-19 | 85.5 |
| MS.gene91206.t1 | MTR_7g445230 | 27.715 | 267 | 120 | 7 | 478 | 742 | 1 | 196 | 9.63e-19 | 85.5 |
| MS.gene91206.t1 | MTR_4g131905 | 43.564 | 101 | 45 | 4 | 45 | 145 | 57 | 145 | 7.68e-15 | 72.8 |
| MS.gene91206.t1 | MTR_2g018360 | 76.923 | 39 | 9 | 0 | 618 | 656 | 30 | 68 | 4.38e-12 | 63.2 |
| MS.gene91206.t1 | MTR_4g082150 | 28.962 | 183 | 118 | 4 | 423 | 596 | 377 | 556 | 4.62e-12 | 70.1 |
| MS.gene91206.t1 | MTR_4g082150 | 28.962 | 183 | 118 | 4 | 423 | 596 | 553 | 732 | 6.36e-12 | 69.7 |
| MS.gene91206.t1 | MTR_7g021880 | 26.768 | 198 | 135 | 3 | 423 | 611 | 581 | 777 | 1.03e-11 | 68.9 |
| MS.gene91206.t1 | MTR_4g082265 | 27.225 | 191 | 129 | 3 | 423 | 604 | 553 | 742 | 1.95e-11 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91206.t1 | AT4G02570 | 80.946 | 740 | 138 | 1 | 3 | 742 | 2 | 738 | 0.0 | 1278 |
| MS.gene91206.t1 | AT4G02570 | 80.946 | 740 | 138 | 1 | 3 | 742 | 2 | 738 | 0.0 | 1278 |
| MS.gene91206.t1 | AT4G02570 | 80.946 | 740 | 138 | 1 | 3 | 742 | 2 | 738 | 0.0 | 1278 |
| MS.gene91206.t1 | AT4G02570 | 80.946 | 740 | 138 | 1 | 3 | 742 | 2 | 738 | 0.0 | 1278 |
| MS.gene91206.t1 | AT1G02980 | 65.054 | 744 | 256 | 4 | 1 | 742 | 1 | 742 | 0.0 | 1028 |
| MS.gene91206.t1 | AT1G02980 | 65.054 | 744 | 256 | 4 | 1 | 742 | 1 | 742 | 0.0 | 1028 |
| MS.gene91206.t1 | AT1G43140 | 65.405 | 740 | 225 | 3 | 4 | 742 | 12 | 721 | 0.0 | 1005 |
| MS.gene91206.t1 | AT1G59790 | 57.552 | 384 | 142 | 3 | 1 | 382 | 6 | 370 | 6.25e-153 | 449 |
| MS.gene91206.t1 | AT1G59790 | 57.527 | 372 | 137 | 3 | 1 | 370 | 6 | 358 | 1.45e-147 | 436 |
| MS.gene91206.t1 | AT1G26830 | 34.312 | 749 | 434 | 14 | 10 | 742 | 26 | 732 | 1.17e-134 | 415 |
| MS.gene91206.t1 | AT1G69670 | 35.120 | 709 | 423 | 12 | 43 | 742 | 52 | 732 | 4.13e-134 | 414 |
| MS.gene91206.t1 | AT1G69670 | 35.120 | 709 | 423 | 12 | 43 | 742 | 52 | 732 | 4.13e-134 | 414 |
| MS.gene91206.t1 | AT5G46210 | 34.123 | 718 | 423 | 16 | 37 | 742 | 113 | 792 | 1.16e-110 | 354 |
| MS.gene91206.t1 | AT1G59800 | 54.098 | 244 | 108 | 1 | 7 | 250 | 7 | 246 | 1.59e-88 | 279 |
| MS.gene91206.t1 | AT2G04660 | 27.041 | 196 | 133 | 2 | 423 | 611 | 543 | 735 | 8.39e-11 | 66.2 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 15184 | gene | 6405 | 14364 | 6405 | ID=MS.gene91206 |
| 15184 | mRNA | 6405 | 14364 | 6405 | ID=MS.gene91206.t1;Parent=MS.gene91206 |
| 15184 | exon | 6405 | 6538 | 6405 | ID=MS.gene91206.t1.exon1;Parent=MS.gene91206.t1 |
| 15184 | CDS | 6405 | 6538 | 6405 | ID=cds.MS.gene91206.t1;Parent=MS.gene91206.t1 |
| 15184 | exon | 6630 | 6729 | 6630 | ID=MS.gene91206.t1.exon2;Parent=MS.gene91206.t1 |
| 15184 | CDS | 6630 | 6729 | 6630 | ID=cds.MS.gene91206.t1;Parent=MS.gene91206.t1 |
| 15184 | exon | 9082 | 9267 | 9082 | ID=MS.gene91206.t1.exon3;Parent=MS.gene91206.t1 |
| 15184 | CDS | 9082 | 9267 | 9082 | ID=cds.MS.gene91206.t1;Parent=MS.gene91206.t1 |
| 15184 | exon | 9377 | 9424 | 9377 | ID=MS.gene91206.t1.exon4;Parent=MS.gene91206.t1 |
| 15184 | CDS | 9377 | 9424 | 9377 | ID=cds.MS.gene91206.t1;Parent=MS.gene91206.t1 |
| 15184 | exon | 9506 | 9709 | 9506 | ID=MS.gene91206.t1.exon5;Parent=MS.gene91206.t1 |
| 15184 | CDS | 9506 | 9709 | 9506 | ID=cds.MS.gene91206.t1;Parent=MS.gene91206.t1 |
| 15184 | exon | 9783 | 9860 | 9783 | ID=MS.gene91206.t1.exon6;Parent=MS.gene91206.t1 |
| 15184 | CDS | 9783 | 9860 | 9783 | ID=cds.MS.gene91206.t1;Parent=MS.gene91206.t1 |
| 15184 | exon | 10031 | 10120 | 10031 | ID=MS.gene91206.t1.exon7;Parent=MS.gene91206.t1 |
| 15184 | CDS | 10031 | 10120 | 10031 | ID=cds.MS.gene91206.t1;Parent=MS.gene91206.t1 |
| 15184 | exon | 10208 | 10288 | 10208 | ID=MS.gene91206.t1.exon8;Parent=MS.gene91206.t1 |
| 15184 | CDS | 10208 | 10288 | 10208 | ID=cds.MS.gene91206.t1;Parent=MS.gene91206.t1 |
| 15184 | exon | 10413 | 10475 | 10413 | ID=MS.gene91206.t1.exon9;Parent=MS.gene91206.t1 |
| 15184 | CDS | 10413 | 10475 | 10413 | ID=cds.MS.gene91206.t1;Parent=MS.gene91206.t1 |
| 15184 | exon | 11522 | 11557 | 11522 | ID=MS.gene91206.t1.exon10;Parent=MS.gene91206.t1 |
| 15184 | CDS | 11522 | 11557 | 11522 | ID=cds.MS.gene91206.t1;Parent=MS.gene91206.t1 |
| 15184 | exon | 11644 | 11730 | 11644 | ID=MS.gene91206.t1.exon11;Parent=MS.gene91206.t1 |
| 15184 | CDS | 11644 | 11730 | 11644 | ID=cds.MS.gene91206.t1;Parent=MS.gene91206.t1 |
| 15184 | exon | 11803 | 11952 | 11803 | ID=MS.gene91206.t1.exon12;Parent=MS.gene91206.t1 |
| 15184 | CDS | 11803 | 11952 | 11803 | ID=cds.MS.gene91206.t1;Parent=MS.gene91206.t1 |
| 15184 | exon | 12041 | 12096 | 12041 | ID=MS.gene91206.t1.exon13;Parent=MS.gene91206.t1 |
| 15184 | CDS | 12041 | 12096 | 12041 | ID=cds.MS.gene91206.t1;Parent=MS.gene91206.t1 |
| 15184 | exon | 12908 | 13028 | 12908 | ID=MS.gene91206.t1.exon14;Parent=MS.gene91206.t1 |
| 15184 | CDS | 12908 | 13028 | 12908 | ID=cds.MS.gene91206.t1;Parent=MS.gene91206.t1 |
| 15184 | exon | 13129 | 13290 | 13129 | ID=MS.gene91206.t1.exon15;Parent=MS.gene91206.t1 |
| 15184 | CDS | 13129 | 13290 | 13129 | ID=cds.MS.gene91206.t1;Parent=MS.gene91206.t1 |
| 15184 | exon | 13397 | 13540 | 13397 | ID=MS.gene91206.t1.exon16;Parent=MS.gene91206.t1 |
| 15184 | CDS | 13397 | 13540 | 13397 | ID=cds.MS.gene91206.t1;Parent=MS.gene91206.t1 |
| 15184 | exon | 13676 | 13894 | 13676 | ID=MS.gene91206.t1.exon17;Parent=MS.gene91206.t1 |
| 15184 | CDS | 13676 | 13894 | 13676 | ID=cds.MS.gene91206.t1;Parent=MS.gene91206.t1 |
| 15184 | exon | 14006 | 14173 | 14006 | ID=MS.gene91206.t1.exon18;Parent=MS.gene91206.t1 |
| 15184 | CDS | 14006 | 14173 | 14006 | ID=cds.MS.gene91206.t1;Parent=MS.gene91206.t1 |
| 15184 | exon | 14263 | 14364 | 14263 | ID=MS.gene91206.t1.exon19;Parent=MS.gene91206.t1 |
| 15184 | CDS | 14263 | 14364 | 14263 | ID=cds.MS.gene91206.t1;Parent=MS.gene91206.t1 |
| Gene Sequence |
| Protein sequence |
