AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35971

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35971.t1 MTR_8g056030 97.923 674 14 0 1 674 58 731 0.0 1374
MS.gene35971.t1 MTR_5g039880 81.454 674 125 0 1 674 59 732 0.0 1157
MS.gene35971.t1 MTR_7g039690 77.893 674 125 1 1 674 59 708 0.0 1093
MS.gene35971.t1 MTR_7g039660 77.893 674 138 2 1 674 58 720 0.0 1089
MS.gene35971.t1 MTR_6g011700 74.926 674 145 1 1 674 56 705 0.0 1037
MS.gene35971.t1 MTR_6g011770 71.810 674 166 1 1 674 56 705 0.0 989
MS.gene35971.t1 MTR_1g040925 75.753 598 127 2 79 674 360 941 0.0 932
MS.gene35971.t1 MTR_1g040925 72.881 295 80 0 1 295 65 359 1.21e-146 450
MS.gene35971.t1 MTR_1g040995 77.586 522 115 1 79 598 360 881 0.0 838
MS.gene35971.t1 MTR_1g040995 72.881 295 80 0 1 295 65 359 5.17e-147 449
MS.gene35971.t1 MTR_5g039770 56.490 678 267 6 1 674 1 654 0.0 763
MS.gene35971.t1 MTR_7g039790 56.765 680 261 7 1 674 65 717 0.0 760
MS.gene35971.t1 MTR_7g445240 55.030 676 263 6 1 674 65 701 0.0 728
MS.gene35971.t1 MTR_5g040040 64.773 528 79 5 147 674 166 586 0.0 657
MS.gene35971.t1 MTR_5g039890 69.853 408 78 2 1 363 56 463 0.0 569
MS.gene35971.t1 MTR_2g019260 41.194 687 371 14 1 674 126 792 8.39e-168 500
MS.gene35971.t1 MTR_5g040180 71.885 313 87 1 1 313 48 359 1.11e-162 472
MS.gene35971.t1 MTR_7g039680 71.207 323 69 3 1 322 56 355 1.56e-158 461
MS.gene35971.t1 MTR_2g019260 40.553 651 356 13 1 638 126 758 2.44e-154 465
MS.gene35971.t1 MTR_7g039440 71.622 296 83 1 18 313 1 295 2.78e-153 445
MS.gene35971.t1 MTR_7g039430 71.886 281 78 1 1 281 48 327 1.06e-142 420
MS.gene35971.t1 MTR_5g040190 68.683 281 87 1 1 281 48 327 3.37e-135 401
MS.gene35971.t1 MTR_7g108340 35.878 655 392 11 38 674 91 735 1.83e-130 402
MS.gene35971.t1 MTR_2g437390 34.564 677 409 12 22 674 76 742 1.40e-129 400
MS.gene35971.t1 MTR_4g119413 35.000 660 397 11 38 674 94 744 8.47e-127 393
MS.gene35971.t1 MTR_1g040965 81.019 216 41 0 459 674 3 218 1.43e-122 363
MS.gene35971.t1 MTR_4g119413 33.065 496 306 9 38 510 94 586 1.26e-85 281
MS.gene35971.t1 MTR_5g040230 72.956 159 43 0 1 159 109 267 7.83e-77 247
MS.gene35971.t1 MTR_7g039380 71.698 159 45 0 1 159 48 206 1.59e-76 244
MS.gene35971.t1 MTR_7g039770 59.434 106 41 1 406 509 1 106 3.37e-37 137
MS.gene35971.t1 MTR_7g039770 64.130 92 33 0 583 674 105 196 1.52e-30 119
MS.gene35971.t1 MTR_7g445230 59.434 106 41 1 406 509 1 106 3.37e-37 137
MS.gene35971.t1 MTR_7g445230 64.130 92 33 0 583 674 105 196 1.52e-30 119
MS.gene35971.t1 MTR_5g039820 44.512 164 51 2 511 674 1 124 7.54e-32 120
MS.gene35971.t1 MTR_1g099430 40.870 115 30 2 560 674 19 95 8.00e-17 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35971.t1 AT1G26830 81.602 674 124 0 1 674 59 732 0.0 1177
MS.gene35971.t1 AT1G69670 80.564 674 131 0 1 674 59 732 0.0 1163
MS.gene35971.t1 AT1G69670 80.564 674 131 0 1 674 59 732 0.0 1163
MS.gene35971.t1 AT5G46210 40.703 683 380 13 1 674 126 792 1.90e-159 479
MS.gene35971.t1 AT4G02570 35.258 658 395 11 38 674 91 738 5.05e-132 406
MS.gene35971.t1 AT4G02570 35.258 658 395 11 38 674 91 738 5.05e-132 406
MS.gene35971.t1 AT4G02570 35.258 658 395 11 38 674 91 738 5.05e-132 406
MS.gene35971.t1 AT4G02570 35.258 658 395 11 38 674 91 738 5.05e-132 406
MS.gene35971.t1 AT1G02980 33.234 674 416 12 25 674 79 742 5.11e-116 365
MS.gene35971.t1 AT1G02980 33.234 674 416 12 25 674 79 742 5.11e-116 365
MS.gene35971.t1 AT1G43140 32.263 685 418 16 6 674 67 721 1.10e-103 332
MS.gene35971.t1 AT1G59790 26.962 293 201 6 22 308 79 364 3.93e-29 120
MS.gene35971.t1 AT1G59790 27.530 247 170 5 22 262 79 322 1.38e-26 112
MS.gene35971.t1 AT1G59800 29.775 178 120 2 22 196 72 247 3.78e-22 97.1
MS.gene35971.t1 AT4G12100 22.388 268 181 8 6 259 118 372 1.89e-12 70.5

Find 137 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGGATCCTTCAATCTTTC+AGG 0.169905 8.2:-41304341 None:intergenic
AATATGGTGCTTCACAAATT+TGG 0.198790 8.2:+41303546 None:intergenic
CACAACACTCAATGAATCTT+TGG 0.228824 8.2:-41304010 None:intergenic
TACTCATCGGCTCCTTTCTA+AGG 0.269735 8.2:-41305198 None:intergenic
TTAATCCATATGGAGAATTC+TGG 0.298338 8.2:+41304173 MS.gene35971:CDS
AAACTATACAAGGTTAAAAT+AGG 0.302125 8.2:+41305271 MS.gene35971:CDS
ACAATCATTGGCTTTAGTTA+AGG 0.303291 8.2:+41305165 MS.gene35971:CDS
TGATGATAAATATGAAGATT+TGG 0.316741 8.2:+41304214 MS.gene35971:CDS
CAATCATTGGCTTTAGTTAA+GGG 0.333046 8.2:+41305166 MS.gene35971:CDS
ACGAGGAATTCAAAGCATTC+TGG 0.343800 8.2:-41304445 None:intergenic
TCAATTTACATCAAAATTAG+AGG 0.346590 8.2:+41304739 MS.gene35971:CDS
GCAGACTTAAAAGCAACCTT+TGG 0.354772 8.2:+41305004 MS.gene35971:CDS
TCGGCTACTGAACACACTTC+TGG 0.360939 8.2:+41303833 MS.gene35971:CDS
CTGCACTTTGCGAGAAGTTT+CGG 0.362050 8.2:+41304921 MS.gene35971:CDS
TAAGAGATGTCTACAATCAT+TGG 0.371194 8.2:+41305153 MS.gene35971:CDS
CTTAACAATGAGACTTCTTT+CGG 0.374056 8.2:-41304696 None:intergenic
ATGCGTTTCTTGACCTCTGT+TGG 0.385513 8.2:-41305478 None:intergenic
AGTTTCCTGTTTCTCAGGTT+CGG 0.385761 8.2:-41305317 None:intergenic
TGCCGAGTTTCCTGTTTCTC+AGG 0.386423 8.2:-41305322 None:intergenic
TGGAAGAACTGAACAGGAAA+TGG 0.387376 8.2:+41303661 MS.gene35971:CDS
CGAAATGAACTCTGGAGAAC+GGG 0.394174 8.2:-41304492 None:intergenic
ACTGTCCAGGTTCTTACAAC+AGG 0.395150 8.2:+41304848 MS.gene35971:CDS
GTAGGACCGTCGCCTAAGTC+AGG 0.411159 8.2:-41304821 None:intergenic
GGAATCCGAACCTGAGAAAC+AGG 0.414007 8.2:+41305312 MS.gene35971:CDS
TCATTTATTCCAACCAGATC+AGG 0.420558 8.2:+41303808 MS.gene35971:CDS
ACAACACTCAATGAATCTTT+GGG 0.423919 8.2:-41304009 None:intergenic
CAGCATTATTGAAAAGCATA+AGG 0.426039 8.2:-41305075 None:intergenic
GATGATAAATATGAAGATTT+GGG 0.429458 8.2:+41304215 MS.gene35971:CDS
CATGCTCAGATTAATCCATA+TGG 0.434059 8.2:+41304163 MS.gene35971:CDS
ACTTCACACATAAGGGAATC+TGG 0.436605 8.2:+41304299 MS.gene35971:CDS
AGGAACTGTAGTTGCACAAA+AGG 0.436933 8.2:+41305291 MS.gene35971:CDS
GTCGCCTAAGTCAGGGTGGC+TGG 0.440169 8.2:-41304813 None:intergenic
TAGTGAGGATCATGAAATCT+AGG 0.440737 8.2:+41305386 MS.gene35971:CDS
TACTGATCCTGAAAGATTGA+AGG 0.441993 8.2:+41304334 MS.gene35971:CDS
AACTCGGCAGCGAGTGGAGG+AGG 0.446158 8.2:+41305336 MS.gene35971:CDS
AAAGCATAAGGACACACATT+TGG 0.448174 8.2:-41305063 None:intergenic
GGCAGCGAGTGGAGGAGGAC+AGG 0.448240 8.2:+41305341 MS.gene35971:CDS
GAAGTATTATAAACAGCATC+TGG 0.448249 8.2:+41304634 MS.gene35971:CDS
CTAAACCAGAATTCTCCATA+TGG 0.453764 8.2:-41304178 None:intergenic
ACTTGTCGTTGTTGAATGCC+AGG 0.462459 8.2:-41304418 None:intergenic
TATGCCAGCCACCCTGACTT+AGG 0.464316 8.2:+41304809 MS.gene35971:CDS
TGCTTCACAAATTTGGTGAT+AGG 0.469912 8.2:+41303553 MS.gene35971:CDS
TGTAGATGATAAACTTCGTA+AGG 0.470956 8.2:+41304520 MS.gene35971:CDS
AGCAACTGAGATTCCTGCTC+CGG 0.471327 8.2:+41305126 MS.gene35971:CDS
AGGTGATGACTTCACACATA+AGG 0.478646 8.2:+41304291 MS.gene35971:CDS
TATTACCTTGGTACACATAC+TGG 0.479679 8.2:+41304947 MS.gene35971:CDS
CAGTCTCGGGATCCATGTAA+TGG 0.479832 8.2:-41304094 None:intergenic
ACTGGTGAAGTTATTGATAG+AGG 0.485538 8.2:+41303876 MS.gene35971:CDS
AACCTCTCAGGACACAATGC+AGG 0.487006 8.2:+41304781 MS.gene35971:CDS
TGAAAAGAAGATTAATAAAG+TGG 0.488330 8.2:+41304115 MS.gene35971:CDS
TCAGTAGCCGAGTCCTGATC+TGG 0.488597 8.2:-41303821 None:intergenic
AGGTTCTTACAACAGGGTCT+TGG 0.488962 8.2:+41304855 MS.gene35971:CDS
TTCATTGAGTGTTGTGATTG+TGG 0.489826 8.2:+41304017 MS.gene35971:CDS
TTCAGCCCGAGTTCATGAAC+AGG 0.490428 8.2:-41303768 None:intergenic
GAGGCGTCTGAATGAGGAAA+TGG 0.490900 8.2:+41304061 MS.gene35971:CDS
CGGTCCTACGCTTACTGTCC+AGG 0.496060 8.2:+41304835 MS.gene35971:CDS
AACTTCGTAAGGGTCTGAAA+GGG 0.499438 8.2:+41304531 MS.gene35971:CDS
GGTGATGACTTCACACATAA+GGG 0.500163 8.2:+41304292 MS.gene35971:CDS
CAGACTTAAAAGCAACCTTT+GGG 0.500900 8.2:+41305005 MS.gene35971:CDS
TGATAGGTTGTACTCAGGAT+TGG 0.503047 8.2:+41303569 MS.gene35971:CDS
AAGACTCCTGTTCATGAACT+CGG 0.516778 8.2:+41303762 MS.gene35971:CDS
TGTAAATTGATAGCCACATT+CGG 0.522404 8.2:-41304726 None:intergenic
GTGCAGATATTTCCACTGGC+AGG 0.524214 8.2:-41304904 None:intergenic
TGACCTCTGTTGGATTTGCA+AGG 0.526865 8.2:-41305468 None:intergenic
AAACTTCGTAAGGGTCTGAA+AGG 0.527114 8.2:+41304530 MS.gene35971:CDS
GGGAAGAAATGTCCTTAGAA+AGG 0.527346 8.2:+41305186 MS.gene35971:CDS
ACAAATAGCGAAATGAACTC+TGG 0.528938 8.2:-41304500 None:intergenic
AGCTGAGAGGCGTCTGAATG+AGG 0.532045 8.2:+41304055 MS.gene35971:CDS
TGTAGACATCTCTTAAGATC+CGG 0.532519 8.2:-41305145 None:intergenic
CTGGTGAAGTTATTGATAGA+GGG 0.538344 8.2:+41303877 MS.gene35971:CDS
AGCCTCTGCACAAACTCGAC+TGG 0.547116 8.2:-41304359 None:intergenic
TACAGACATGAAAACCTCTC+AGG 0.548291 8.2:+41304769 MS.gene35971:CDS
ATGAACTCGGGCTGAATCTG+TGG 0.550756 8.2:+41303775 MS.gene35971:CDS
GGATAGAGTTAGCCATTACA+TGG 0.551642 8.2:+41304082 MS.gene35971:CDS
AAGACCCTGTTGTAAGAACC+TGG 0.553834 8.2:-41304853 None:intergenic
TTTGGTGATAGGTTGTACTC+AGG 0.562234 8.2:+41303564 MS.gene35971:CDS
CTTAAAAGCAACCTTTGGGA+AGG 0.563218 8.2:+41305009 MS.gene35971:CDS
ATTGTCATGGCAAACTAACA+TGG 0.566165 8.2:+41304976 MS.gene35971:CDS
AAATCTATTGAAGCAGCTCA+AGG 0.568181 8.2:+41303627 MS.gene35971:CDS
TGCTGATAAACTTAGCTACA+AGG 0.569495 8.2:+41305093 MS.gene35971:CDS
AGACTCCTGTTCATGAACTC+GGG 0.571232 8.2:+41303763 MS.gene35971:CDS
CATCTCTTAAGATCCGGAGC+AGG 0.571367 8.2:-41305139 None:intergenic
CAAAGTGCAGATATTTCCAC+TGG 0.572612 8.2:-41304908 None:intergenic
ACTATTGCCGCCTCGATCTG+AGG 0.574443 8.2:-41305367 None:intergenic
GCGAAATGAACTCTGGAGAA+CGG 0.579728 8.2:-41304493 None:intergenic
ACAGGAAACTCGGCAGCGAG+TGG 0.580836 8.2:+41305330 MS.gene35971:CDS
CAATTTACATCAAAATTAGA+GGG 0.584508 8.2:+41304740 MS.gene35971:CDS
AGCCACCCTGACTTAGGCGA+CGG 0.585562 8.2:+41304815 MS.gene35971:CDS
TTAAAAGCAACCTTTGGGAA+GGG 0.586426 8.2:+41305010 MS.gene35971:CDS
ATAAATATGAAGATTTGGGT+AGG 0.586773 8.2:+41304219 MS.gene35971:CDS
ATACGACAAGATTATAAACC+TGG 0.588266 8.2:+41304400 MS.gene35971:CDS
ACCTTGGTACACATACTGGC+AGG 0.589264 8.2:+41304951 MS.gene35971:CDS
GGATGATGTAGAGGTTACTC+TGG 0.590036 8.2:+41304562 MS.gene35971:CDS
AAGTTTCGGTCATATTACCT+TGG 0.591080 8.2:+41304935 MS.gene35971:CDS
CATAAGGACACACATTTGGT+AGG 0.591138 8.2:-41305059 None:intergenic
AACCTGAGAAACAGGAAACT+CGG 0.594659 8.2:+41305320 MS.gene35971:CDS
CATGTAATACTAGACTGAGT+TGG 0.597381 8.2:-41304878 None:intergenic
ATTCCAACCAGATCAGGACT+CGG 0.601328 8.2:+41303814 MS.gene35971:CDS
ATGCTTCTGACCCTTCCCAA+AGG 0.602178 8.2:-41305020 None:intergenic
GGACAGGAAGCCTCAGATCG+AGG 0.603180 8.2:+41305357 MS.gene35971:CDS
TCACCAACATCTTTACTCAT+CGG 0.604309 8.2:-41305211 None:intergenic
TAGAGATATACTGATGTACA+TGG 0.604770 8.2:+41303713 MS.gene35971:CDS
AATTCTTGAGCATAAACAGA+TGG 0.606533 8.2:-41303939 None:intergenic
GTAGATGATAAACTTCGTAA+GGG 0.607058 8.2:+41304521 MS.gene35971:CDS
CTGTCCAGGTTCTTACAACA+GGG 0.608852 8.2:+41304849 MS.gene35971:CDS
ACCTCTCAGGACACAATGCA+GGG 0.612857 8.2:+41304782 MS.gene35971:CDS
TAGGACCGTCGCCTAAGTCA+GGG 0.612941 8.2:-41304820 None:intergenic
GGAAACTCGGCAGCGAGTGG+AGG 0.615424 8.2:+41305333 MS.gene35971:CDS
GTTAAGCTCAAGACCGAATG+TGG 0.616613 8.2:+41304713 MS.gene35971:CDS
AGATCGAGGCGGCAATAGTG+AGG 0.619757 8.2:+41305371 MS.gene35971:CDS
AAAGAAGATTAATAAAGTGG+TGG 0.620743 8.2:+41304118 MS.gene35971:CDS
TAGCCGAGTCCTGATCTGGT+TGG 0.622232 8.2:-41303817 None:intergenic
ATAAACAGCATCTGGCAAAG+AGG 0.630265 8.2:+41304642 MS.gene35971:CDS
TTGTTTCGTCGTGTAACCGA+CGG 0.634022 8.2:+41304251 MS.gene35971:CDS
GTTCAGTAGTAAACTATACA+AGG 0.638422 8.2:+41305261 MS.gene35971:CDS
GAAATGAACTCTGGAGAACG+GGG 0.638998 8.2:-41304491 None:intergenic
GGTCAACGAGGATGATGTAG+AGG 0.645668 8.2:+41304553 MS.gene35971:CDS
GAGCCGATGAGTAAAGATGT+TGG 0.646356 8.2:+41305208 MS.gene35971:CDS
ACTTCGTAAGGGTCTGAAAG+GGG 0.649523 8.2:+41304532 MS.gene35971:CDS
TTGTCATGGCAAACTAACAT+GGG 0.650035 8.2:+41304977 MS.gene35971:CDS
TCCTGCCAGTATGTGTACCA+AGG 0.652600 8.2:-41304952 None:intergenic
TATTACATGCAACCTGCCAG+TGG 0.653340 8.2:+41304892 MS.gene35971:CDS
GCCCTGCATTGTGTCCTGAG+AGG 0.655976 8.2:-41304783 None:intergenic
TTAGTACATAGTGAACGCAC+TGG 0.661997 8.2:+41303858 MS.gene35971:CDS
TGTAGAGGTTACTCTGGACA+AGG 0.664682 8.2:+41304568 MS.gene35971:CDS
ATCCAGTCGAGTTTGTGCAG+AGG 0.686131 8.2:+41304357 MS.gene35971:CDS
GACCGTCGCCTAAGTCAGGG+TGG 0.696070 8.2:-41304817 None:intergenic
TTTCCTTGCAAATCCAACAG+AGG 0.698003 8.2:+41305465 MS.gene35971:CDS
CAGGAAGCCTCAGATCGAGG+CGG 0.699496 8.2:+41305360 MS.gene35971:CDS
ATACTGGCAGGAGATTGTCA+TGG 0.705494 8.2:+41304963 MS.gene35971:CDS
AGAACCTGGACAGTAAGCGT+AGG 0.706663 8.2:-41304839 None:intergenic
TCTATTGAAGCAGCTCAAGG+AGG 0.716304 8.2:+41303630 MS.gene35971:CDS
GGGTCTGAAAGGGGTCAACG+AGG 0.729389 8.2:+41304541 MS.gene35971:CDS
GTTAATGAAATATTCAAACG+AGG 0.733246 8.2:-41304462 None:intergenic
GATGAGTAAAGATGTTGGTG+AGG 0.734708 8.2:+41305213 MS.gene35971:CDS
AAATGAACTCTGGAGAACGG+GGG 0.745398 8.2:-41304490 None:intergenic
ATTATCTTAAGAAAGCTGAG+AGG 0.761790 8.2:+41304042 MS.gene35971:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATAACAAAAATGCTAA+TGG + chr8.2:41303908-41303927 MS.gene35971:CDS 15.0%
!! AAACTATACAAGGTTAAAAT+AGG + chr8.2:41305271-41305290 MS.gene35971:CDS 20.0%
!! CAATTTACATCAAAATTAGA+GGG + chr8.2:41304740-41304759 MS.gene35971:CDS 20.0%
!! GATGATAAATATGAAGATTT+GGG + chr8.2:41304215-41304234 MS.gene35971:CDS 20.0%
!! TCAATTTACATCAAAATTAG+AGG + chr8.2:41304739-41304758 MS.gene35971:CDS 20.0%
!! TGAAAAGAAGATTAATAAAG+TGG + chr8.2:41304115-41304134 MS.gene35971:CDS 20.0%
!! TGATGATAAATATGAAGATT+TGG + chr8.2:41304214-41304233 MS.gene35971:CDS 20.0%
! AAAGAAGATTAATAAAGTGG+TGG + chr8.2:41304118-41304137 MS.gene35971:CDS 25.0%
! ATAAATATGAAGATTTGGGT+AGG + chr8.2:41304219-41304238 MS.gene35971:CDS 25.0%
! GTTAATGAAATATTCAAACG+AGG - chr8.2:41304465-41304484 None:intergenic 25.0%
!! ATTAATCTTCTTTTCAGTCT+CGG - chr8.2:41304111-41304130 None:intergenic 25.0%
!!! ACAAAAATGCTAATGGATTT+AGG + chr8.2:41303915-41303934 MS.gene35971:CDS 25.0%
AATTCTTGAGCATAAACAGA+TGG - chr8.2:41303942-41303961 None:intergenic 30.0%
ACAACACTCAATGAATCTTT+GGG - chr8.2:41304012-41304031 None:intergenic 30.0%
ATACGACAAGATTATAAACC+TGG + chr8.2:41304400-41304419 MS.gene35971:CDS 30.0%
ATTATCTTAAGAAAGCTGAG+AGG + chr8.2:41304042-41304061 MS.gene35971:CDS 30.0%
CTTAACAATGAGACTTCTTT+CGG - chr8.2:41304699-41304718 None:intergenic 30.0%
GTAGATGATAAACTTCGTAA+GGG + chr8.2:41304521-41304540 MS.gene35971:CDS 30.0%
GTTCAGTAGTAAACTATACA+AGG + chr8.2:41305261-41305280 MS.gene35971:CDS 30.0%
TAAGAGATGTCTACAATCAT+TGG + chr8.2:41305153-41305172 MS.gene35971:CDS 30.0%
TAGAGATATACTGATGTACA+TGG + chr8.2:41303713-41303732 MS.gene35971:CDS 30.0%
TGTAGATGATAAACTTCGTA+AGG + chr8.2:41304520-41304539 MS.gene35971:CDS 30.0%
TTAATCCATATGGAGAATTC+TGG + chr8.2:41304173-41304192 MS.gene35971:CDS 30.0%
! CAGCATTATTGAAAAGCATA+AGG - chr8.2:41305078-41305097 None:intergenic 30.0%
! CATCTTTTTCTTGCAAGTAT+CGG - chr8.2:41304607-41304626 None:intergenic 30.0%
! GAAGTATTATAAACAGCATC+TGG + chr8.2:41304634-41304653 MS.gene35971:CDS 30.0%
! TGTAAATTGATAGCCACATT+CGG - chr8.2:41304729-41304748 None:intergenic 30.0%
! TTAATCTTCTTTTCAGTCTC+GGG - chr8.2:41304110-41304129 None:intergenic 30.0%
!! ACAATCATTGGCTTTAGTTA+AGG + chr8.2:41305165-41305184 MS.gene35971:CDS 30.0%
!! CAATCATTGGCTTTAGTTAA+GGG + chr8.2:41305166-41305185 MS.gene35971:CDS 30.0%
AAAGCATAAGGACACACATT+TGG - chr8.2:41305066-41305085 None:intergenic 35.0%
AAATCTATTGAAGCAGCTCA+AGG + chr8.2:41303627-41303646 MS.gene35971:CDS 35.0%
AAGTTTCGGTCATATTACCT+TGG + chr8.2:41304935-41304954 MS.gene35971:CDS 35.0%
ACAAATAGCGAAATGAACTC+TGG - chr8.2:41304503-41304522 None:intergenic 35.0%
ACTGGTGAAGTTATTGATAG+AGG + chr8.2:41303876-41303895 MS.gene35971:CDS 35.0%
ATTGTCATGGCAAACTAACA+TGG + chr8.2:41304976-41304995 MS.gene35971:CDS 35.0%
CACAACACTCAATGAATCTT+TGG - chr8.2:41304013-41304032 None:intergenic 35.0%
CAGACTTAAAAGCAACCTTT+GGG + chr8.2:41305005-41305024 MS.gene35971:CDS 35.0%
CATGCTCAGATTAATCCATA+TGG + chr8.2:41304163-41304182 MS.gene35971:CDS 35.0%
CATGTAATACTAGACTGAGT+TGG - chr8.2:41304881-41304900 None:intergenic 35.0%
CTAAACCAGAATTCTCCATA+TGG - chr8.2:41304181-41304200 None:intergenic 35.0%
CTGGTGAAGTTATTGATAGA+GGG + chr8.2:41303877-41303896 MS.gene35971:CDS 35.0%
TACTGATCCTGAAAGATTGA+AGG + chr8.2:41304334-41304353 MS.gene35971:CDS 35.0%
TAGTGAGGATCATGAAATCT+AGG + chr8.2:41305386-41305405 MS.gene35971:CDS 35.0%
TATTACCTTGGTACACATAC+TGG + chr8.2:41304947-41304966 MS.gene35971:CDS 35.0%
TCACCAACATCTTTACTCAT+CGG - chr8.2:41305214-41305233 None:intergenic 35.0%
TCATTTATTCCAACCAGATC+AGG + chr8.2:41303808-41303827 MS.gene35971:CDS 35.0%
TGCTGATAAACTTAGCTACA+AGG + chr8.2:41305093-41305112 MS.gene35971:CDS 35.0%
TGTAGACATCTCTTAAGATC+CGG - chr8.2:41305148-41305167 None:intergenic 35.0%
TTGTCATGGCAAACTAACAT+GGG + chr8.2:41304977-41304996 MS.gene35971:CDS 35.0%
! TCGTATTTTCAAAAGACCGT+CGG - chr8.2:41304270-41304289 None:intergenic 35.0%
! TGCTTCACAAATTTGGTGAT+AGG + chr8.2:41303553-41303572 MS.gene35971:CDS 35.0%
! TTAAAAGCAACCTTTGGGAA+GGG + chr8.2:41305010-41305029 MS.gene35971:CDS 35.0%
! TTCATGAACAGGAGTCTTTT+TGG - chr8.2:41303760-41303779 None:intergenic 35.0%
! TTCATTGAGTGTTGTGATTG+TGG + chr8.2:41304017-41304036 MS.gene35971:CDS 35.0%
!! AGTTTCAGCTGAGTTTTATC+AGG + chr8.2:41303980-41303999 MS.gene35971:CDS 35.0%
AAACTTCGTAAGGGTCTGAA+AGG + chr8.2:41304530-41304549 MS.gene35971:CDS 40.0%
AACCTGAGAAACAGGAAACT+CGG + chr8.2:41305320-41305339 MS.gene35971:CDS 40.0%
AACTTCGTAAGGGTCTGAAA+GGG + chr8.2:41304531-41304550 MS.gene35971:CDS 40.0%
AAGACTCCTGTTCATGAACT+CGG + chr8.2:41303762-41303781 MS.gene35971:CDS 40.0%
ACTGGATCCTTCAATCTTTC+AGG - chr8.2:41304344-41304363 None:intergenic 40.0%
ACTTCACACATAAGGGAATC+TGG + chr8.2:41304299-41304318 MS.gene35971:CDS 40.0%
AGGAACTGTAGTTGCACAAA+AGG + chr8.2:41305291-41305310 MS.gene35971:CDS 40.0%
AGGTGATGACTTCACACATA+AGG + chr8.2:41304291-41304310 MS.gene35971:CDS 40.0%
ATAAACAGCATCTGGCAAAG+AGG + chr8.2:41304642-41304661 MS.gene35971:CDS 40.0%
CAAAGTGCAGATATTTCCAC+TGG - chr8.2:41304911-41304930 None:intergenic 40.0%
CATAAGGACACACATTTGGT+AGG - chr8.2:41305062-41305081 None:intergenic 40.0%
GATGAGTAAAGATGTTGGTG+AGG + chr8.2:41305213-41305232 MS.gene35971:CDS 40.0%
GCAGACTTAAAAGCAACCTT+TGG + chr8.2:41305004-41305023 MS.gene35971:CDS 40.0%
GGATAGAGTTAGCCATTACA+TGG + chr8.2:41304082-41304101 MS.gene35971:CDS 40.0%
GGGAAGAAATGTCCTTAGAA+AGG + chr8.2:41305186-41305205 MS.gene35971:CDS 40.0%
GGTGATGACTTCACACATAA+GGG + chr8.2:41304292-41304311 MS.gene35971:CDS 40.0%
TACAGACATGAAAACCTCTC+AGG + chr8.2:41304769-41304788 MS.gene35971:CDS 40.0%
TGATAGGTTGTACTCAGGAT+TGG + chr8.2:41303569-41303588 MS.gene35971:CDS 40.0%
TGGAAGAACTGAACAGGAAA+TGG + chr8.2:41303661-41303680 MS.gene35971:CDS 40.0%
TTAGTACATAGTGAACGCAC+TGG + chr8.2:41303858-41303877 MS.gene35971:CDS 40.0%
TTTCCTTGCAAATCCAACAG+AGG + chr8.2:41305465-41305484 MS.gene35971:CDS 40.0%
! ACGAGGAATTCAAAGCATTC+TGG - chr8.2:41304448-41304467 None:intergenic 40.0%
! AGTTTCCTGTTTCTCAGGTT+CGG - chr8.2:41305320-41305339 None:intergenic 40.0%
! CTTAAAAGCAACCTTTGGGA+AGG + chr8.2:41305009-41305028 MS.gene35971:CDS 40.0%
! GAGGCTTTTGTCTGGAAAAA+CGG + chr8.2:41304661-41304680 MS.gene35971:CDS 40.0%
! TCTCATTGAGCGAGACTTTT+TGG + chr8.2:41305507-41305526 MS.gene35971:CDS 40.0%
! TTTGGTGATAGGTTGTACTC+AGG + chr8.2:41303564-41303583 MS.gene35971:CDS 40.0%
!! CGGTCTTTTGAAAATACGAG+AGG + chr8.2:41304271-41304290 MS.gene35971:CDS 40.0%
!! CTTTTCTGGAAGAACTGAAC+AGG + chr8.2:41303655-41303674 MS.gene35971:CDS 40.0%
AAGACCCTGTTGTAAGAACC+TGG - chr8.2:41304856-41304875 None:intergenic 45.0%
ACTGTCCAGGTTCTTACAAC+AGG + chr8.2:41304848-41304867 MS.gene35971:CDS 45.0%
ACTTCGTAAGGGTCTGAAAG+GGG + chr8.2:41304532-41304551 MS.gene35971:CDS 45.0%
ACTTGTCGTTGTTGAATGCC+AGG - chr8.2:41304421-41304440 None:intergenic 45.0%
AGACTCCTGTTCATGAACTC+GGG + chr8.2:41303763-41303782 MS.gene35971:CDS 45.0%
AGGTTCTTACAACAGGGTCT+TGG + chr8.2:41304855-41304874 MS.gene35971:CDS 45.0%
ATACTGGCAGGAGATTGTCA+TGG + chr8.2:41304963-41304982 MS.gene35971:CDS 45.0%
ATGCGTTTCTTGACCTCTGT+TGG - chr8.2:41305481-41305500 None:intergenic 45.0%
ATTCCAACCAGATCAGGACT+CGG + chr8.2:41303814-41303833 MS.gene35971:CDS 45.0%
CTGCACTTTGCGAGAAGTTT+CGG + chr8.2:41304921-41304940 MS.gene35971:CDS 45.0%
CTGTCCAGGTTCTTACAACA+GGG + chr8.2:41304849-41304868 MS.gene35971:CDS 45.0%
GAGCCGATGAGTAAAGATGT+TGG + chr8.2:41305208-41305227 MS.gene35971:CDS 45.0%
GGATGATGTAGAGGTTACTC+TGG + chr8.2:41304562-41304581 MS.gene35971:CDS 45.0%
GTTAAGCTCAAGACCGAATG+TGG + chr8.2:41304713-41304732 MS.gene35971:CDS 45.0%
TATTACATGCAACCTGCCAG+TGG + chr8.2:41304892-41304911 MS.gene35971:CDS 45.0%
TCTATTGAAGCAGCTCAAGG+AGG + chr8.2:41303630-41303649 MS.gene35971:CDS 45.0%
TGACCTCTGTTGGATTTGCA+AGG - chr8.2:41305471-41305490 None:intergenic 45.0%
TGTAGAGGTTACTCTGGACA+AGG + chr8.2:41304568-41304587 MS.gene35971:CDS 45.0%
TTGTTTCGTCGTGTAACCGA+CGG + chr8.2:41304251-41304270 MS.gene35971:CDS 45.0%
! AAATGAACTCTGGAGAACGG+GGG - chr8.2:41304493-41304512 None:intergenic 45.0%
! AGCTCAAGGAGGATCTTTTC+TGG + chr8.2:41303641-41303660 MS.gene35971:CDS 45.0%
! CGAAATGAACTCTGGAGAAC+GGG - chr8.2:41304495-41304514 None:intergenic 45.0%
! GAAATGAACTCTGGAGAACG+GGG - chr8.2:41304494-41304513 None:intergenic 45.0%
! GCGAAATGAACTCTGGAGAA+CGG - chr8.2:41304496-41304515 None:intergenic 45.0%
!! ACAGGAGTCTTTTTGGCACT+TGG - chr8.2:41303753-41303772 None:intergenic 45.0%
!! TACTCATCGGCTCCTTTCTA+AGG - chr8.2:41305201-41305220 None:intergenic 45.0%
!! TGAGCGAGACTTTTTGGAGA+GGG + chr8.2:41305513-41305532 MS.gene35971:CDS 45.0%
!! TTGAGCGAGACTTTTTGGAG+AGG + chr8.2:41305512-41305531 MS.gene35971:CDS 45.0%
AACCTCTCAGGACACAATGC+AGG + chr8.2:41304781-41304800 MS.gene35971:CDS 50.0%
ACCTCTCAGGACACAATGCA+GGG + chr8.2:41304782-41304801 MS.gene35971:CDS 50.0%
ACCTTGGTACACATACTGGC+AGG + chr8.2:41304951-41304970 MS.gene35971:CDS 50.0%
AGAACCTGGACAGTAAGCGT+AGG - chr8.2:41304842-41304861 None:intergenic 50.0%
AGCAACTGAGATTCCTGCTC+CGG + chr8.2:41305126-41305145 MS.gene35971:CDS 50.0%
ATCCAGTCGAGTTTGTGCAG+AGG + chr8.2:41304357-41304376 MS.gene35971:CDS 50.0%
ATGAACTCGGGCTGAATCTG+TGG + chr8.2:41303775-41303794 MS.gene35971:CDS 50.0%
ATGCTTCTGACCCTTCCCAA+AGG - chr8.2:41305023-41305042 None:intergenic 50.0%
CAGTCTCGGGATCCATGTAA+TGG - chr8.2:41304097-41304116 None:intergenic 50.0%
CATCTCTTAAGATCCGGAGC+AGG - chr8.2:41305142-41305161 None:intergenic 50.0%
GGAATCCGAACCTGAGAAAC+AGG + chr8.2:41305312-41305331 MS.gene35971:CDS 50.0%
GGTCAACGAGGATGATGTAG+AGG + chr8.2:41304553-41304572 MS.gene35971:CDS 50.0%
GTGCAGATATTTCCACTGGC+AGG - chr8.2:41304907-41304926 None:intergenic 50.0%
TCCTGCCAGTATGTGTACCA+AGG - chr8.2:41304955-41304974 None:intergenic 50.0%
TCGGCTACTGAACACACTTC+TGG + chr8.2:41303833-41303852 MS.gene35971:CDS 50.0%
TTCAGCCCGAGTTCATGAAC+AGG - chr8.2:41303771-41303790 None:intergenic 50.0%
! CTGGCAAAGAGGCTTTTGTC+TGG + chr8.2:41304653-41304672 MS.gene35971:CDS 50.0%
! GAGGCGTCTGAATGAGGAAA+TGG + chr8.2:41304061-41304080 MS.gene35971:CDS 50.0%
! TGCCGAGTTTCCTGTTTCTC+AGG - chr8.2:41305325-41305344 None:intergenic 50.0%
ACTATTGCCGCCTCGATCTG+AGG - chr8.2:41305370-41305389 None:intergenic 55.0%
AGATCGAGGCGGCAATAGTG+AGG + chr8.2:41305371-41305390 MS.gene35971:CDS 55.0%
AGCCTCTGCACAAACTCGAC+TGG - chr8.2:41304362-41304381 None:intergenic 55.0%
TAGCCGAGTCCTGATCTGGT+TGG - chr8.2:41303820-41303839 None:intergenic 55.0%
TAGGACCGTCGCCTAAGTCA+GGG - chr8.2:41304823-41304842 None:intergenic 55.0%
TATGCCAGCCACCCTGACTT+AGG + chr8.2:41304809-41304828 MS.gene35971:CDS 55.0%
TCAGTAGCCGAGTCCTGATC+TGG - chr8.2:41303824-41303843 None:intergenic 55.0%
!! AGCTGAGAGGCGTCTGAATG+AGG + chr8.2:41304055-41304074 MS.gene35971:CDS 55.0%
ACAGGAAACTCGGCAGCGAG+TGG + chr8.2:41305330-41305349 MS.gene35971:CDS 60.0%
AGCCACCCTGACTTAGGCGA+CGG + chr8.2:41304815-41304834 MS.gene35971:CDS 60.0%
CAGGAAGCCTCAGATCGAGG+CGG + chr8.2:41305360-41305379 MS.gene35971:CDS 60.0%
CGGTCCTACGCTTACTGTCC+AGG + chr8.2:41304835-41304854 MS.gene35971:CDS 60.0%
GCCCTGCATTGTGTCCTGAG+AGG - chr8.2:41304786-41304805 None:intergenic 60.0%
GGACAGGAAGCCTCAGATCG+AGG + chr8.2:41305357-41305376 MS.gene35971:CDS 60.0%
GGGTCTGAAAGGGGTCAACG+AGG + chr8.2:41304541-41304560 MS.gene35971:CDS 60.0%
GTAGGACCGTCGCCTAAGTC+AGG - chr8.2:41304824-41304843 None:intergenic 60.0%
AACTCGGCAGCGAGTGGAGG+AGG + chr8.2:41305336-41305355 MS.gene35971:CDS 65.0%
GACCGTCGCCTAAGTCAGGG+TGG - chr8.2:41304820-41304839 None:intergenic 65.0%
GGAAACTCGGCAGCGAGTGG+AGG + chr8.2:41305333-41305352 MS.gene35971:CDS 65.0%
GTCGCCTAAGTCAGGGTGGC+TGG - chr8.2:41304816-41304835 None:intergenic 65.0%
GGCAGCGAGTGGAGGAGGAC+AGG + chr8.2:41305341-41305360 MS.gene35971:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 41303549 41305573 41303549 ID=MS.gene35971
chr8.2 mRNA 41303549 41305573 41303549 ID=MS.gene35971.t1;Parent=MS.gene35971
chr8.2 exon 41303549 41305573 41303549 ID=MS.gene35971.t1.exon1;Parent=MS.gene35971.t1
chr8.2 CDS 41303549 41305573 41303549 ID=cds.MS.gene35971.t1;Parent=MS.gene35971.t1
Gene Sequence

>MS.gene35971

ATGGTGCTTCACAAATTTGGTGATAGGTTGTACTCAGGATTGGTTGCGACTATGACTGCGCATCTCAAAGAAATAGCTAAATCTATTGAAGCAGCTCAAGGAGGATCTTTTCTGGAAGAACTGAACAGGAAATGGAATGATCATAACAAAGCATTGCAAATGATTAGAGATATACTGATGTACATGGACAGAACTTTCATTCCAAGTGCCAAAAAGACTCCTGTTCATGAACTCGGGCTGAATCTGTGGAAAGAAAATGTCATTTATTCCAACCAGATCAGGACTCGGCTACTGAACACACTTCTGGAATTAGTACATAGTGAACGCACTGGTGAAGTTATTGATAGAGGGATAATGAGAAATATAACAAAAATGCTAATGGATTTAGGTCCATCTGTTTATGCTCAAGAATTTGAGACTCATTTTCTTCAAGTTTCAGCTGAGTTTTATCAGGTAGAGTCCCAAAGATTCATTGAGTGTTGTGATTGTGGTGATTATCTTAAGAAAGCTGAGAGGCGTCTGAATGAGGAAATGGATAGAGTTAGCCATTACATGGATCCCGAGACTGAAAAGAAGATTAATAAAGTGGTGGAGACGCAGATGATTGAAAATCACATGCTCAGATTAATCCATATGGAGAATTCTGGTTTAGTCAACATGCTTTGTGATGATAAATATGAAGATTTGGGTAGGATGTATAACTTGTTTCGTCGTGTAACCGACGGTCTTTTGAAAATACGAGAGGTGATGACTTCACACATAAGGGAATCTGGTAAACAGCTTGTTACTGATCCTGAAAGATTGAAGGATCCAGTCGAGTTTGTGCAGAGGCTCTTAGATGAGAAAGACAAATACGACAAGATTATAAACCTGGCATTCAACAACGACAAGTCTTTCCAGAATGCTTTGAATTCCTCGTTTGAATATTTCATTAACTTGAACCCCCGTTCTCCAGAGTTCATTTCGCTATTTGTAGATGATAAACTTCGTAAGGGTCTGAAAGGGGTCAACGAGGATGATGTAGAGGTTACTCTGGACAAGGTGATGATGCTGTTCCGATACTTGCAAGAAAAAGATGTTTTTGAGAAGTATTATAAACAGCATCTGGCAAAGAGGCTTTTGTCTGGAAAAACGGTTTCTGATGATGCCGAAAGAAGTCTCATTGTTAAGCTCAAGACCGAATGTGGCTATCAATTTACATCAAAATTAGAGGGAATGTTTACAGACATGAAAACCTCTCAGGACACAATGCAGGGCTTTTATGCCAGCCACCCTGACTTAGGCGACGGTCCTACGCTTACTGTCCAGGTTCTTACAACAGGGTCTTGGCCAACTCAGTCTAGTATTACATGCAACCTGCCAGTGGAAATATCTGCACTTTGCGAGAAGTTTCGGTCATATTACCTTGGTACACATACTGGCAGGAGATTGTCATGGCAAACTAACATGGGCTTTGCAGACTTAAAAGCAACCTTTGGGAAGGGTCAGAAGCATGAGTTAAATGTCTCTACCTACCAAATGTGTGTCCTTATGCTTTTCAATAATGCTGATAAACTTAGCTACAAGGAGATTGAGCAAGCAACTGAGATTCCTGCTCCGGATCTTAAGAGATGTCTACAATCATTGGCTTTAGTTAAGGGAAGAAATGTCCTTAGAAAGGAGCCGATGAGTAAAGATGTTGGTGAGGATGATGCTTTCTCTGTTAATGACAAGTTCAGTAGTAAACTATACAAGGTTAAAATAGGAACTGTAGTTGCACAAAAGGAATCCGAACCTGAGAAACAGGAAACTCGGCAGCGAGTGGAGGAGGACAGGAAGCCTCAGATCGAGGCGGCAATAGTGAGGATCATGAAATCTAGGAAGCAACTTGATCATAACAATCTTATAGCTGAAGTCACAAAGCAGTTGCAGTCACGTTTCCTTGCAAATCCAACAGAGGTCAAGAAACGCATTGAGTCTCTCATTGAGCGAGACTTTTTGGAGAGGGATGATAACGACAGAAAAATGTATCGATATCTTGCATAG
Protein sequence

>MS.gene35971.t1

MVLHKFGDRLYSGLVATMTAHLKEIAKSIEAAQGGSFLEELNRKWNDHNKALQMIRDILMYMDRTFIPSAKKTPVHELGLNLWKENVIYSNQIRTRLLNTLLELVHSERTGEVIDRGIMRNITKMLMDLGPSVYAQEFETHFLQVSAEFYQVESQRFIECCDCGDYLKKAERRLNEEMDRVSHYMDPETEKKINKVVETQMIENHMLRLIHMENSGLVNMLCDDKYEDLGRMYNLFRRVTDGLLKIREVMTSHIRESGKQLVTDPERLKDPVEFVQRLLDEKDKYDKIINLAFNNDKSFQNALNSSFEYFINLNPRSPEFISLFVDDKLRKGLKGVNEDDVEVTLDKVMMLFRYLQEKDVFEKYYKQHLAKRLLSGKTVSDDAERSLIVKLKTECGYQFTSKLEGMFTDMKTSQDTMQGFYASHPDLGDGPTLTVQVLTTGSWPTQSSITCNLPVEISALCEKFRSYYLGTHTGRRLSWQTNMGFADLKATFGKGQKHELNVSTYQMCVLMLFNNADKLSYKEIEQATEIPAPDLKRCLQSLALVKGRNVLRKEPMSKDVGEDDAFSVNDKFSSKLYKVKIGTVVAQKESEPEKQETRQRVEEDRKPQIEAAIVRIMKSRKQLDHNNLIAEVTKQLQSRFLANPTEVKKRIESLIERDFLERDDNDRKMYRYLA