Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91539.t1 | XP_003591056.1 | 93.6 | 110 | 5 | 2 | 1 | 109 | 184 | 292 | 1.10E-52 | 215.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91539.t1 | Q9MAA2 | 64.0 | 89 | 32 | 0 | 1 | 89 | 188 | 276 | 5.8e-31 | 134.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91539.t1 | G7I7H1 | 93.6 | 110 | 5 | 2 | 1 | 109 | 184 | 292 | 7.7e-53 | 215.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene91539 | 0.800098 | 1.69E-48 | -1.69E-46 |
| MS.gene04998 | MS.gene91539 | 0.815371 | 9.57E-52 | -1.69E-46 |
| MS.gene050945 | MS.gene91539 | 0.809249 | 2.08E-50 | -1.69E-46 |
| MS.gene051043 | MS.gene91539 | 0.818326 | 2.08E-52 | -1.69E-46 |
| MS.gene051226 | MS.gene91539 | 0.851025 | 1.16E-60 | -1.69E-46 |
| MS.gene051699 | MS.gene91539 | 0.801925 | 7.15E-49 | -1.69E-46 |
| MS.gene051959 | MS.gene91539 | 0.803156 | 3.99E-49 | -1.69E-46 |
| MS.gene051960 | MS.gene91539 | 0.802925 | 4.45E-49 | -1.69E-46 |
| MS.gene052296 | MS.gene91539 | 0.816253 | 6.08E-52 | -1.69E-46 |
| MS.gene054893 | MS.gene91539 | 0.81014 | 1.34E-50 | -1.69E-46 |
| MS.gene057093 | MS.gene91539 | 0.822692 | 2.07E-53 | -1.69E-46 |
| MS.gene057622 | MS.gene91539 | 0.818758 | 1.66E-52 | -1.69E-46 |
| MS.gene060964 | MS.gene91539 | 0.82425 | 8.94E-54 | -1.69E-46 |
| MS.gene061100 | MS.gene91539 | 0.804363 | 2.24E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91539.t1 | MTR_1g082360 | 93.636 | 110 | 5 | 2 | 1 | 109 | 184 | 292 | 3.55e-70 | 211 |
| MS.gene91539.t1 | MTR_7g113920 | 62.136 | 103 | 36 | 1 | 1 | 103 | 216 | 315 | 8.53e-43 | 142 |
| MS.gene91539.t1 | MTR_5g010090 | 46.512 | 86 | 45 | 1 | 1 | 86 | 262 | 346 | 1.93e-21 | 87.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91539.t1 | AT3G05170 | 64.045 | 89 | 32 | 0 | 1 | 89 | 188 | 276 | 4.20e-37 | 127 |
| MS.gene91539.t1 | AT1G08940 | 61.957 | 92 | 32 | 2 | 2 | 92 | 192 | 281 | 5.80e-33 | 116 |
Find 26 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCAGTTTCACCTTCATTT+TGG | 0.169991 | 1.2:+60569563 | None:intergenic |
| TTTCGGAAACTGCGAGTTTC+GGG | 0.182047 | 1.2:-60569797 | MS.gene91539:CDS |
| TTTGAGCATCTAAACAATTT+CGG | 0.293790 | 1.2:-60569814 | MS.gene91539:CDS |
| ATTTCGGAAACTGCGAGTTT+CGG | 0.331893 | 1.2:-60569798 | MS.gene91539:CDS |
| CAATGTCCTTGGTATCTTGA+TGG | 0.384985 | 1.2:-60569634 | MS.gene91539:CDS |
| CGGAAGAAGAGATGCTTGAA+TGG | 0.421203 | 1.2:-60569726 | MS.gene91539:CDS |
| GGAAGAAGAGATGCTTGAAT+GGG | 0.451438 | 1.2:-60569725 | MS.gene91539:CDS |
| GCTTGAATGGGGAATGTCTC+CGG | 0.465250 | 1.2:-60569713 | MS.gene91539:CDS |
| ATATGATTGCTGATCAGAAA+TGG | 0.466739 | 1.2:-60569690 | MS.gene91539:CDS |
| TTCTGATCAGCAATCATATC+CGG | 0.479044 | 1.2:+60569694 | None:intergenic |
| AAACTGCGAGTTTCGGGTGA+TGG | 0.492323 | 1.2:-60569791 | MS.gene91539:CDS |
| CGGGTGATGGAGCAAGGGAG+TGG | 0.510953 | 1.2:-60569778 | MS.gene91539:CDS |
| CAGTTTGGCGATTAATCATA+CGG | 0.511425 | 1.2:-60569746 | MS.gene91539:CDS |
| GATGAAGCAATCCAAAATGA+AGG | 0.532371 | 1.2:-60569574 | MS.gene91539:CDS |
| GAGTTTCGGGTGATGGAGCA+AGG | 0.542115 | 1.2:-60569784 | MS.gene91539:CDS |
| GAGTGGTGGAGAATACAGTT+TGG | 0.544566 | 1.2:-60569761 | MS.gene91539:CDS |
| GTGATGGAGCAAGGGAGTGG+TGG | 0.558690 | 1.2:-60569775 | MS.gene91539:CDS |
| TGATCATTCCAAGCACCTCT+AGG | 0.566827 | 1.2:+60569655 | None:intergenic |
| CAGATGCGCCTAGAGGTGCT+TGG | 0.570632 | 1.2:-60569663 | MS.gene91539:CDS |
| CTTGGAATGATCAATGTCCT+TGG | 0.578748 | 1.2:-60569645 | MS.gene91539:CDS |
| TCTTGATGAAGTGGTTCAAG+TGG | 0.585341 | 1.2:-60569849 | None:intergenic |
| AGTTTCGGGTGATGGAGCAA+GGG | 0.652564 | 1.2:-60569783 | MS.gene91539:CDS |
| TGGCGTGCAGATGCGCCTAG+AGG | 0.658589 | 1.2:-60569670 | MS.gene91539:CDS |
| GAAGAAGAGATGCTTGAATG+GGG | 0.659893 | 1.2:-60569724 | MS.gene91539:CDS |
| GATGAAGTGGTTCAAGTGGA+CGG | 0.691694 | 1.2:-60569845 | MS.gene91539:CDS |
| GAAGTGGTTCAAGTGGACGG+TGG | 0.719720 | 1.2:-60569842 | MS.gene91539:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTGAGCATCTAAACAATTT+CGG | - | chr1.2:60569567-60569586 | MS.gene91539:CDS | 25.0% |
| AAAAAACCATCAAGATACCA+AGG | + | chr1.2:60569756-60569775 | None:intergenic | 30.0% | |
| ATATGATTGCTGATCAGAAA+TGG | - | chr1.2:60569691-60569710 | MS.gene91539:CDS | 30.0% | |
| ! | TTTCAGTTTCACCTTCATTT+TGG | + | chr1.2:60569821-60569840 | None:intergenic | 30.0% |
| CAGTTTGGCGATTAATCATA+CGG | - | chr1.2:60569635-60569654 | MS.gene91539:CDS | 35.0% | |
| GATGAAGCAATCCAAAATGA+AGG | - | chr1.2:60569807-60569826 | MS.gene91539:CDS | 35.0% | |
| TTCTGATCAGCAATCATATC+CGG | + | chr1.2:60569690-60569709 | None:intergenic | 35.0% | |
| ATTTCGGAAACTGCGAGTTT+CGG | - | chr1.2:60569583-60569602 | MS.gene91539:CDS | 40.0% | |
| CAATGTCCTTGGTATCTTGA+TGG | - | chr1.2:60569747-60569766 | MS.gene91539:CDS | 40.0% | |
| CTTGGAATGATCAATGTCCT+TGG | - | chr1.2:60569736-60569755 | MS.gene91539:CDS | 40.0% | |
| GAAGAAGAGATGCTTGAATG+GGG | - | chr1.2:60569657-60569676 | MS.gene91539:CDS | 40.0% | |
| GGAAGAAGAGATGCTTGAAT+GGG | - | chr1.2:60569656-60569675 | MS.gene91539:CDS | 40.0% | |
| CGGAAGAAGAGATGCTTGAA+TGG | - | chr1.2:60569655-60569674 | MS.gene91539:CDS | 45.0% | |
| TGATCATTCCAAGCACCTCT+AGG | + | chr1.2:60569729-60569748 | None:intergenic | 45.0% | |
| TTTCGGAAACTGCGAGTTTC+GGG | - | chr1.2:60569584-60569603 | MS.gene91539:CDS | 45.0% | |
| ! | GAGTGGTGGAGAATACAGTT+TGG | - | chr1.2:60569620-60569639 | MS.gene91539:CDS | 45.0% |
| GCTTGAATGGGGAATGTCTC+CGG | - | chr1.2:60569668-60569687 | MS.gene91539:CDS | 50.0% | |
| ! | AAACTGCGAGTTTCGGGTGA+TGG | - | chr1.2:60569590-60569609 | MS.gene91539:CDS | 50.0% |
| !! | AGTTTCGGGTGATGGAGCAA+GGG | - | chr1.2:60569598-60569617 | MS.gene91539:CDS | 50.0% |
| GAAGTGGTTCAAGTGGACGG+TGG | - | chr1.2:60569539-60569558 | MS.gene91539:CDS | 55.0% | |
| !! | GAGTTTCGGGTGATGGAGCA+AGG | - | chr1.2:60569597-60569616 | MS.gene91539:CDS | 55.0% |
| ! | GTGATGGAGCAAGGGAGTGG+TGG | - | chr1.2:60569606-60569625 | MS.gene91539:CDS | 60.0% |
| !! | CAGATGCGCCTAGAGGTGCT+TGG | - | chr1.2:60569718-60569737 | MS.gene91539:CDS | 60.0% |
| TGGCGTGCAGATGCGCCTAG+AGG | - | chr1.2:60569711-60569730 | MS.gene91539:CDS | 65.0% | |
| !! | CGGGTGATGGAGCAAGGGAG+TGG | - | chr1.2:60569603-60569622 | MS.gene91539:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 60569537 | 60569866 | 60569537 | ID=MS.gene91539 |
| chr1.2 | mRNA | 60569537 | 60569866 | 60569537 | ID=MS.gene91539.t1;Parent=MS.gene91539 |
| chr1.2 | exon | 60569537 | 60569866 | 60569537 | ID=MS.gene91539.t1.exon1;Parent=MS.gene91539.t1 |
| chr1.2 | CDS | 60569537 | 60569866 | 60569537 | ID=cds.MS.gene91539.t1;Parent=MS.gene91539.t1 |
| Gene Sequence |
| Protein sequence |
