Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92990.t1 | XP_003603744.1 | 90.5 | 273 | 26 | 0 | 13 | 285 | 1 | 273 | 4.70E-140 | 507.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92990.t1 | O81769 | 75.4 | 272 | 66 | 1 | 13 | 283 | 1 | 272 | 1.4e-121 | 437.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92990.t1 | G7J4Q4 | 90.5 | 273 | 26 | 0 | 13 | 285 | 1 | 273 | 3.4e-140 | 507.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene92990 | MS.gene83565 | PPI |
| MS.gene92990 | MS.gene067444 | PPI |
| MS.gene92990 | MS.gene85931 | PPI |
| MS.gene92990 | MS.gene86968 | PPI |
| MS.gene92990 | MS.gene56983 | PPI |
| MS.gene009798 | MS.gene92990 | PPI |
| MS.gene50211 | MS.gene92990 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92990.t1 | MTR_3g113750 | 90.476 | 273 | 26 | 0 | 13 | 285 | 1 | 273 | 0.0 | 520 |
| MS.gene92990.t1 | MTR_1g107520 | 92.727 | 165 | 8 | 1 | 13 | 177 | 1 | 161 | 3.08e-107 | 309 |
| MS.gene92990.t1 | MTR_3g114210 | 68.243 | 148 | 38 | 3 | 43 | 181 | 1 | 148 | 2.99e-57 | 181 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92990.t1 | AT4G31790 | 74.818 | 274 | 68 | 1 | 13 | 285 | 1 | 274 | 4.12e-160 | 446 |
| MS.gene92990.t1 | AT4G31790 | 74.818 | 274 | 68 | 1 | 13 | 285 | 1 | 274 | 4.12e-160 | 446 |
Find 67 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGCTACAGTTGATTGATTT+TGG | 0.122944 | 1.2:-80280835 | MS.gene92990:CDS |
| TTGCTATGGTTACAGATATT+AGG | 0.197130 | 1.2:-80281718 | MS.gene92990:intron |
| ATGTTATACATAATCGGTTT+AGG | 0.222248 | 1.2:-80289298 | MS.gene92990:CDS |
| CTTCACACACTTTGCTTGTT+AGG | 0.224154 | 1.2:-80288729 | MS.gene92990:intron |
| TGTGTTGATCGTCATGTATC+TGG | 0.257878 | 1.2:+80281382 | None:intergenic |
| TGTTATACATAATCGGTTTA+GGG | 0.275650 | 1.2:-80289297 | MS.gene92990:CDS |
| CATGTTGCTTTCCTTGTTGT+TGG | 0.290249 | 1.2:-80289058 | MS.gene92990:CDS |
| TAAATGCGACAAAGTTTATA+TGG | 0.290999 | 1.2:-80289227 | MS.gene92990:CDS |
| TGCTATGGTTACAGATATTA+GGG | 0.304377 | 1.2:-80281717 | MS.gene92990:intron |
| CTTGTTGTTGGTGACCCTTT+TGG | 0.307530 | 1.2:-80289046 | MS.gene92990:CDS |
| TACATAATCGGTTTAGGGTT+GGG | 0.353222 | 1.2:-80289292 | MS.gene92990:CDS |
| CATGTATCTGGGTGGTTCAT+AGG | 0.357827 | 1.2:+80281394 | None:intergenic |
| GGTTTGCAGCTATATCGTTA+TGG | 0.358551 | 1.2:-80288837 | MS.gene92990:CDS |
| CTGTAGCTGCTTCATTGTTC+CGG | 0.365688 | 1.2:+80280849 | None:intergenic |
| GTTTGCAGCTATATCGTTAT+GGG | 0.367093 | 1.2:-80288836 | MS.gene92990:CDS |
| TTGCATTGCCTAGTCATAAC+AGG | 0.382182 | 1.2:-80280805 | MS.gene92990:CDS |
| TACACTTCTCTTCTCTCCTT+CGG | 0.382807 | 1.2:-80289199 | MS.gene92990:CDS |
| CACAGCCATTGACCAGCTAT+TGG | 0.391767 | 1.2:-80281363 | MS.gene92990:CDS |
| TTCTCTATCTGCAAGTGTAA+TGG | 0.397647 | 1.2:+80289129 | None:intergenic |
| AATAGCTTGTAAACCTTTGA+GGG | 0.412690 | 1.2:+80289252 | None:intergenic |
| AACCTTTGAGGGTGATATCT+TGG | 0.414105 | 1.2:+80289263 | None:intergenic |
| GAATAGCTTGTAAACCTTTG+AGG | 0.424343 | 1.2:+80289251 | None:intergenic |
| TTGAATGCAATTGGGATTTG+TGG | 0.430993 | 1.2:-80288858 | MS.gene92990:CDS |
| AACAGGCAAGACCCATCCTT+TGG | 0.449566 | 1.2:-80280788 | MS.gene92990:CDS |
| TCCTTTGGAGGAAGAAATGC+TGG | 0.451222 | 1.2:-80280773 | MS.gene92990:CDS |
| TACACTTGCAGATAGAGAAA+TGG | 0.452546 | 1.2:-80289125 | MS.gene92990:CDS |
| TCCTTCGGTCTCTCTTCAAA+CGG | 0.456224 | 1.2:-80289184 | MS.gene92990:CDS |
| CTGAAGATACTGAGTGTGTT+GGG | 0.467169 | 1.2:-80280909 | MS.gene92990:CDS |
| TGCTGGATTTCTACAGATGC+AGG | 0.472871 | 1.2:-80280756 | MS.gene92990:CDS |
| AACATGAGAAAGATGAGCTT+GGG | 0.476125 | 1.2:+80289075 | None:intergenic |
| TAGTAGCATACGTACCCAAA+AGG | 0.476909 | 1.2:+80289031 | None:intergenic |
| CTGAGTGTGTTGGGTTTGCT+CGG | 0.479514 | 1.2:-80280900 | MS.gene92990:CDS |
| TCTCCATGTCTCGGTAAAGA+AGG | 0.483323 | 1.2:+80288797 | None:intergenic |
| TGTGTTGGGTTTGCTCGGAT+TGG | 0.491487 | 1.2:-80280895 | MS.gene92990:CDS |
| GCATTTCTTCCTCCAAAGGA+TGG | 0.492628 | 1.2:+80280776 | None:intergenic |
| ATACATAATCGGTTTAGGGT+TGG | 0.507394 | 1.2:-80289293 | MS.gene92990:CDS |
| TGTTGTTCGAGCAAAGAAGA+TGG | 0.512659 | 1.2:-80288916 | MS.gene92990:CDS |
| CAACATGAGAAAGATGAGCT+TGG | 0.518904 | 1.2:+80289074 | None:intergenic |
| CTTGTGCAATCTCCAATAGC+TGG | 0.526744 | 1.2:+80281351 | None:intergenic |
| CCACGCTGGAATCTTTGTGC+AGG | 0.532208 | 1.2:-80281685 | MS.gene92990:intron |
| GTGTTGATCGTCATGTATCT+GGG | 0.540891 | 1.2:+80281383 | None:intergenic |
| TCCAGCATTTCTTCCTCCAA+AGG | 0.541129 | 1.2:+80280772 | None:intergenic |
| GCACAAGCGCATGAAGAAGC+TGG | 0.545697 | 1.2:-80281335 | MS.gene92990:intron |
| CATTTCTTCCTCCAAAGGAT+GGG | 0.556386 | 1.2:+80280777 | None:intergenic |
| AGTAGCATACGTACCCAAAA+GGG | 0.556867 | 1.2:+80289032 | None:intergenic |
| GTTGTTCGAGCAAAGAAGAT+GGG | 0.566000 | 1.2:-80288915 | MS.gene92990:CDS |
| AGGCAAGACCCATCCTTTGG+AGG | 0.566323 | 1.2:-80280785 | MS.gene92990:CDS |
| AATCTCCAATAGCTGGTCAA+TGG | 0.569611 | 1.2:+80281358 | None:intergenic |
| CTCCATGTCTCGGTAAAGAA+GGG | 0.572832 | 1.2:+80288798 | None:intergenic |
| GAAGATCAGAAGGTAGTAGC+CGG | 0.574536 | 1.2:-80280868 | MS.gene92990:CDS |
| TACCCTTCTTTACCGAGACA+TGG | 0.575922 | 1.2:-80288800 | MS.gene92990:CDS |
| TCTGAAGATACTGAGTGTGT+TGG | 0.579919 | 1.2:-80280910 | MS.gene92990:CDS |
| ACACTCAGTATCTTCAGAGT+AGG | 0.583085 | 1.2:+80280915 | None:intergenic |
| GGTTACAGATATTAGGGTGA+AGG | 0.584730 | 1.2:-80281711 | MS.gene92990:intron |
| GCCGTTTGAAGAGAGACCGA+AGG | 0.585888 | 1.2:+80289183 | None:intergenic |
| TTTGATATGACTAGTCAAGA+AGG | 0.586585 | 1.2:-80289340 | None:intergenic |
| GGGTCTTGCCTGTTATGACT+AGG | 0.587259 | 1.2:+80280797 | None:intergenic |
| GCTATCAGGTCTCCATGTCT+CGG | 0.598618 | 1.2:+80288788 | None:intergenic |
| TAGGGTGAAGGAGCCCACGC+TGG | 0.600425 | 1.2:-80281699 | MS.gene92990:CDS |
| GACCAAGATATCACCCTCAA+AGG | 0.601432 | 1.2:-80289265 | MS.gene92990:CDS |
| GATTGGAAGTGAAGATCAGA+AGG | 0.607232 | 1.2:-80280878 | MS.gene92990:CDS |
| CTGCACAAAGATTCCAGCGT+GGG | 0.607705 | 1.2:+80281686 | None:intergenic |
| CAAAAGGGTCACCAACAACA+AGG | 0.612707 | 1.2:+80289047 | None:intergenic |
| TTTGCAGCTATATCGTTATG+GGG | 0.661053 | 1.2:-80288835 | MS.gene92990:CDS |
| GTTATGACTAGGCAATGCAA+AGG | 0.678397 | 1.2:+80280808 | None:intergenic |
| TTGATCGTCATGTATCTGGG+TGG | 0.755594 | 1.2:+80281386 | None:intergenic |
| CCTGCACAAAGATTCCAGCG+TGG | 0.765961 | 1.2:+80281685 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 80280750 | 80289356 | 80280750 | ID=MS.gene92990 |
| chr1.2 | mRNA | 80280750 | 80289356 | 80280750 | ID=MS.gene92990.t1;Parent=MS.gene92990 |
| chr1.2 | exon | 80289046 | 80289356 | 80289046 | ID=MS.gene92990.t1.exon1;Parent=MS.gene92990.t1 |
| chr1.2 | CDS | 80289046 | 80289356 | 80289046 | ID=cds.MS.gene92990.t1;Parent=MS.gene92990.t1 |
| chr1.2 | exon | 80288730 | 80288959 | 80288730 | ID=MS.gene92990.t1.exon2;Parent=MS.gene92990.t1 |
| chr1.2 | CDS | 80288730 | 80288959 | 80288730 | ID=cds.MS.gene92990.t1;Parent=MS.gene92990.t1 |
| chr1.2 | exon | 80281686 | 80281725 | 80281686 | ID=MS.gene92990.t1.exon3;Parent=MS.gene92990.t1 |
| chr1.2 | CDS | 80281686 | 80281725 | 80281686 | ID=cds.MS.gene92990.t1;Parent=MS.gene92990.t1 |
| chr1.2 | exon | 80281336 | 80281427 | 80281336 | ID=MS.gene92990.t1.exon4;Parent=MS.gene92990.t1 |
| chr1.2 | CDS | 80281336 | 80281427 | 80281336 | ID=cds.MS.gene92990.t1;Parent=MS.gene92990.t1 |
| chr1.2 | exon | 80280750 | 80280937 | 80280750 | ID=MS.gene92990.t1.exon5;Parent=MS.gene92990.t1 |
| chr1.2 | CDS | 80280750 | 80280937 | 80280750 | ID=cds.MS.gene92990.t1;Parent=MS.gene92990.t1 |
| Gene Sequence |
| Protein sequence |
