Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94380.t1 | XP_013455261.1 | 95.1 | 618 | 7 | 1 | 1 | 595 | 1 | 618 | 0.00E+00 | 1156.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94380.t1 | Q291H5 | 56.6 | 438 | 178 | 4 | 25 | 453 | 66 | 500 | 1.7e-140 | 501.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94380.t1 | A0A072UHZ4 | 95.1 | 618 | 7 | 1 | 1 | 595 | 1 | 618 | 0.0e+00 | 1156.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene94380 | MS.gene070139 | PPI |
| MS.gene94380 | MS.gene85146 | PPI |
| MS.gene36347 | MS.gene94380 | PPI |
| MS.gene94380 | MS.gene062116 | PPI |
| MS.gene94380 | MS.gene025347 | PPI |
| MS.gene85145 | MS.gene94380 | PPI |
| MS.gene94380 | MS.gene36348 | PPI |
| MS.gene94380 | MS.gene85342 | PPI |
| MS.gene94380 | MS.gene76530 | PPI |
| MS.gene94380 | MS.gene069865 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94380.t1 | MTR_4g033210 | 95.146 | 618 | 7 | 1 | 1 | 595 | 1 | 618 | 0.0 | 1201 |
| MS.gene94380.t1 | MTR_5g021230 | 23.846 | 520 | 308 | 15 | 9 | 452 | 79 | 586 | 3.99e-33 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94380.t1 | AT1G72090 | 65.764 | 628 | 150 | 6 | 1 | 592 | 1 | 599 | 0.0 | 813 |
| MS.gene94380.t1 | AT4G36390 | 23.952 | 501 | 292 | 16 | 29 | 452 | 129 | 617 | 1.24e-31 | 130 |
Find 127 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTGTTGATAAGGCTCTTT+TGG | 0.133036 | 4.3:+67041708 | MS.gene94380:CDS |
| GCTGGTCAGCTTTCAGCTTT+TGG | 0.169840 | 4.3:+67035072 | MS.gene94380:CDS |
| CCAATTAATGTTGGTTGTTT+AGG | 0.201753 | 4.3:+67038036 | MS.gene94380:CDS |
| CCTAAACAACCAACATTAAT+TGG | 0.244538 | 4.3:-67038036 | None:intergenic |
| AAGGCAAAGTGGAAAGAATT+TGG | 0.247160 | 4.3:+67040545 | MS.gene94380:CDS |
| GTCTTAGGTTTAGTGTTCTT+TGG | 0.248640 | 4.3:+67041776 | MS.gene94380:CDS |
| TTGCTTTATTAGTTTGTCTT+AGG | 0.260565 | 4.3:+67041761 | MS.gene94380:CDS |
| CTTCTCTTCACCACATTACT+TGG | 0.264756 | 4.3:-67040469 | None:intergenic |
| TTCAAGTGCTCAAGGATAAA+AGG | 0.293438 | 4.3:-67038678 | None:intergenic |
| GATATAATATGTGGATTTCC+AGG | 0.335774 | 4.3:+67038949 | MS.gene94380:CDS |
| TTCCCACAAGCACAAGATTC+TGG | 0.347283 | 4.3:-67041487 | None:intergenic |
| TTCTGAGATTTCACAAGTCT+TGG | 0.354694 | 4.3:-67041461 | None:intergenic |
| ACCTGCGGCAAGAATGAAAA+AGG | 0.355796 | 4.3:+67040444 | MS.gene94380:CDS |
| CTGATGGAGTTAAAGAAATA+TGG | 0.362385 | 4.3:+67038340 | MS.gene94380:CDS |
| TTGCCTTCCATCCCAGAGTA+TGG | 0.378724 | 4.3:-67040529 | None:intergenic |
| CTGAACTGGCACATGCAAAA+AGG | 0.379014 | 4.3:-67038739 | None:intergenic |
| CAAAGACATTTGTCAATTCA+CGG | 0.382984 | 4.3:-67040495 | None:intergenic |
| CTGGAAGGGATCAGCATTGT+AGG | 0.383351 | 4.3:+67037754 | MS.gene94380:CDS |
| ATCCTGATGAAGCAGATCTA+TGG | 0.386251 | 4.3:+67035112 | MS.gene94380:CDS |
| GAATCATTCACGCCATACTC+TGG | 0.389654 | 4.3:+67040517 | MS.gene94380:CDS |
| AGCTGGTAATTCTGCTACTA+AGG | 0.395120 | 4.3:-67038616 | None:intergenic |
| AAAGGAAGAATCTGCACAAA+AGG | 0.397088 | 4.3:+67041623 | MS.gene94380:CDS |
| GTTGTAGAGGAGACTTTAAA+AGG | 0.399227 | 4.3:+67037805 | MS.gene94380:CDS |
| ACCTTAACTGAGCTTGTGCC+GGG | 0.402416 | 4.3:+67038910 | MS.gene94380:CDS |
| TACAGTGTCCCTTGAATTGT+CGG | 0.404396 | 4.3:-67041548 | None:intergenic |
| CCTTCCCACAGATGTTATCT+TGG | 0.407575 | 4.3:-67041329 | None:intergenic |
| ACAGAGTGATAGTGAATATA+TGG | 0.412525 | 4.3:+67035050 | MS.gene94380:intron |
| AGATTTCACAAGTCTTGGTT+TGG | 0.413329 | 4.3:-67041456 | None:intergenic |
| CAACAACGCGATCAATTTGC+TGG | 0.415734 | 4.3:-67037780 | None:intergenic |
| AAGCGCTGGCAATGTCTTAC+GGG | 0.416449 | 4.3:-67037848 | None:intergenic |
| AAGAAAGGAGCATGAGAAAG+TGG | 0.418448 | 4.3:+67041683 | MS.gene94380:CDS |
| AATAAACCACTAGTTGTTGC+TGG | 0.419430 | 4.3:+67037697 | MS.gene94380:CDS |
| CAAGCGCTGGCAATGTCTTA+CGG | 0.420275 | 4.3:-67037849 | None:intergenic |
| AGCCGCTACCGGAGCTCCAC+CGG | 0.422571 | 4.3:+67034690 | MS.gene94380:CDS |
| ACTAAGGCATTCAATAAAGT+TGG | 0.424272 | 4.3:-67038600 | None:intergenic |
| AGCCATAGATCTGCTTCATC+AGG | 0.432713 | 4.3:-67035114 | None:intergenic |
| CCCCGGCACAAGCTCAGTTA+AGG | 0.435890 | 4.3:-67038911 | None:intergenic |
| AGCATAGTGCTTGCATCAGC+TGG | 0.438177 | 4.3:-67038633 | None:intergenic |
| TTGAGAAGACCGACAATTCA+AGG | 0.440687 | 4.3:+67041539 | MS.gene94380:CDS |
| AAAGTGGGACTTTGTTGATA+AGG | 0.441838 | 4.3:+67041699 | MS.gene94380:CDS |
| CAATAAAGCTTTGAACAAAC+AGG | 0.443124 | 4.3:+67041399 | MS.gene94380:CDS |
| TTGGATGGATACTGAGGAAA+AGG | 0.450909 | 4.3:+67041605 | MS.gene94380:CDS |
| TTGCATGTGCCAGTTCAGTC+TGG | 0.452847 | 4.3:+67038744 | MS.gene94380:CDS |
| TCACATCTCGACTTCCTTGA+GGG | 0.467101 | 4.3:-67037726 | None:intergenic |
| TCCCGGTGGAGCTCCGGTAG+CGG | 0.470158 | 4.3:-67034692 | None:intergenic |
| AGTGAAGATACCGGAGCATA+TGG | 0.472936 | 4.3:+67038369 | MS.gene94380:CDS |
| TACCTTAACTGAGCTTGTGC+CGG | 0.473023 | 4.3:+67038909 | MS.gene94380:CDS |
| ATGGATACCATCATAACAAA+AGG | 0.483104 | 4.3:+67037664 | MS.gene94380:CDS |
| ACGGAATCCCGGTGGAGCTC+CGG | 0.484772 | 4.3:-67034698 | None:intergenic |
| TTCAACGGAGAACGGAATCC+CGG | 0.485616 | 4.3:-67034709 | None:intergenic |
| GAACTTCCAAGCATATCATC+TGG | 0.486695 | 4.3:-67041298 | None:intergenic |
| GCATCACTTCCAGACTGAAC+TGG | 0.492471 | 4.3:-67038753 | None:intergenic |
| GCAAGCACTATGCTGCGTAT+TGG | 0.495273 | 4.3:+67038642 | MS.gene94380:CDS |
| GATCTGATCTAAAGCAAGAA+AGG | 0.497761 | 4.3:+67041668 | MS.gene94380:CDS |
| CTGAAGGTTGGTCATACAAA+GGG | 0.500724 | 4.3:+67041250 | MS.gene94380:intron |
| CTCAAGGATAAAAGGTGGAT+TGG | 0.509294 | 4.3:-67038670 | None:intergenic |
| TTCACATCTCGACTTCCTTG+AGG | 0.511443 | 4.3:-67037727 | None:intergenic |
| GAGCCAGAATCTTGTGCTTG+TGG | 0.516083 | 4.3:+67041484 | MS.gene94380:CDS |
| AGCCAGAATCTTGTGCTTGT+GGG | 0.517247 | 4.3:+67041485 | MS.gene94380:CDS |
| GTATCCATGGCAGATTGACT+TGG | 0.520909 | 4.3:-67037649 | None:intergenic |
| TTGCTCATCGGAGCCGCTAC+CGG | 0.521509 | 4.3:+67034679 | MS.gene94380:CDS |
| TGCAACTGATGGAGTTCACT+TGG | 0.521540 | 4.3:+67040579 | MS.gene94380:CDS |
| ATTGCCACTGATATAATATG+TGG | 0.524293 | 4.3:+67038940 | MS.gene94380:CDS |
| TATCCATGGCAGATTGACTT+GGG | 0.524481 | 4.3:-67037648 | None:intergenic |
| AATCATTCACGCCATACTCT+GGG | 0.529461 | 4.3:+67040518 | MS.gene94380:CDS |
| AAGTCGAGATGTGAAAGAGC+TGG | 0.532706 | 4.3:+67037735 | MS.gene94380:CDS |
| ATTACTGATATTGCAACTGA+TGG | 0.536300 | 4.3:+67040568 | MS.gene94380:CDS |
| GAACAAACAGGTTCCTACTC+AGG | 0.537765 | 4.3:+67041411 | MS.gene94380:CDS |
| GACATTGAAGATTTGCTCAT+CGG | 0.539895 | 4.3:+67034667 | MS.gene94380:CDS |
| CTTTAAAAGGTCACGAAGTG+AGG | 0.543654 | 4.3:+67037818 | MS.gene94380:CDS |
| CTTCTTCGTTGCAACGCCGA+CGG | 0.544956 | 4.3:-67034740 | None:intergenic |
| ATTGCTCCAGATGATATGCT+TGG | 0.546633 | 4.3:+67041292 | MS.gene94380:CDS |
| CTATCTCTTTCAAGTGCTCA+AGG | 0.546786 | 4.3:-67038686 | None:intergenic |
| TATCTATTGTGTAACTCCCT+AGG | 0.546949 | 4.3:-67038098 | None:intergenic |
| TTTGAATTCATACCTTTGGA+AGG | 0.548972 | 4.3:-67037873 | None:intergenic |
| AGTTTGCTCAAAATCTTCAT+CGG | 0.549560 | 4.3:-67039347 | None:intergenic |
| CGTTGCAACGCCGACGGTAA+CGG | 0.550069 | 4.3:-67034734 | None:intergenic |
| ATTTGTTTGGATGGATACTG+AGG | 0.551888 | 4.3:+67041599 | MS.gene94380:CDS |
| TAACGGAGTTCAACGGAGAA+CGG | 0.554457 | 4.3:-67034717 | None:intergenic |
| GTAGAAAGTGAACTTGCCTC+AGG | 0.554829 | 4.3:+67041646 | MS.gene94380:CDS |
| CTCTGGGATGGAAGGCAAAG+TGG | 0.556380 | 4.3:+67040534 | MS.gene94380:CDS |
| GCCGCTACCGGAGCTCCACC+GGG | 0.560907 | 4.3:+67034691 | MS.gene94380:CDS |
| GAGATTCTTCCAATTAATGT+TGG | 0.562618 | 4.3:+67038027 | MS.gene94380:CDS |
| ACCTTTGGAAGGTCAAGCGC+TGG | 0.564190 | 4.3:-67037862 | None:intergenic |
| AAGGAAGAATCTGCACAAAA+GGG | 0.564610 | 4.3:+67041624 | MS.gene94380:CDS |
| GTTGCTGGCTGTGTCCCTCA+AGG | 0.566523 | 4.3:+67037712 | MS.gene94380:CDS |
| ACGCCATACTCTGGGATGGA+AGG | 0.569239 | 4.3:+67040526 | MS.gene94380:CDS |
| TAATTATTGGTGCAGTTGCA+CGG | 0.571250 | 4.3:+67037618 | MS.gene94380:intron |
| GTAAAATCTGTGGTATCTGA+TGG | 0.573159 | 4.3:+67038324 | MS.gene94380:CDS |
| CCGTTGAACTCCGTTACCGT+CGG | 0.574263 | 4.3:+67034724 | MS.gene94380:CDS |
| CGAGATGTGAAAGAGCTGGA+AGG | 0.575668 | 4.3:+67037739 | MS.gene94380:CDS |
| TCAGTGGCAATCTGCATCCC+CGG | 0.575983 | 4.3:-67038928 | None:intergenic |
| TTAGAAATTACCATATGCTC+CGG | 0.576298 | 4.3:-67038379 | None:intergenic |
| TATGCTTGGAAGTTCAGCTC+TGG | 0.580362 | 4.3:+67041306 | MS.gene94380:CDS |
| ATTTGGGTGTTCTCATAACC+AGG | 0.580770 | 4.3:+67034916 | MS.gene94380:CDS |
| AGTGATAGTGAATATATGGC+TGG | 0.587060 | 4.3:+67035054 | MS.gene94380:CDS |
| GTCCCCAAGTCAATCTGCCA+TGG | 0.587573 | 4.3:+67037645 | MS.gene94380:CDS |
| AAACATGCTCGAGGTCACCT+AGG | 0.587931 | 4.3:+67038081 | MS.gene94380:CDS |
| TCGCGTTGTTGAAGTTGTAG+AGG | 0.590773 | 4.3:+67037792 | MS.gene94380:CDS |
| CCAGATCACACACCTGGTCG+AGG | 0.591497 | 4.3:-67039424 | None:intergenic |
| GAGATGTGAAAGAGCTGGAA+GGG | 0.591544 | 4.3:+67037740 | MS.gene94380:CDS |
| TGAGAAGACCGACAATTCAA+GGG | 0.600489 | 4.3:+67041540 | MS.gene94380:CDS |
| CTGGCCAAGATAACATCTGT+GGG | 0.605676 | 4.3:+67041325 | MS.gene94380:CDS |
| AAGTGCTCAAGGATAAAAGG+TGG | 0.609787 | 4.3:-67038675 | None:intergenic |
| AGATAACATCTGTGGGAAGG+TGG | 0.611853 | 4.3:+67041332 | MS.gene94380:CDS |
| AACATGCTCGAGGTCACCTA+GGG | 0.613729 | 4.3:+67038082 | MS.gene94380:CDS |
| ATTCACGCCATACTCTGGGA+TGG | 0.613962 | 4.3:+67040522 | MS.gene94380:CDS |
| AGAAAGGAGCATGAGAAAGT+GGG | 0.617963 | 4.3:+67041684 | MS.gene94380:CDS |
| GCTGAAGGTTGGTCATACAA+AGG | 0.618455 | 4.3:+67041249 | MS.gene94380:intron |
| AGAGAAGACATATCCTGAGT+AGG | 0.619463 | 4.3:-67041424 | None:intergenic |
| TTGCTTTAGATCAGATCCTG+AGG | 0.623187 | 4.3:-67041662 | None:intergenic |
| AAATCCACATATTATATCAG+TGG | 0.624202 | 4.3:-67038944 | None:intergenic |
| CCAAGATAACATCTGTGGGA+AGG | 0.625892 | 4.3:+67041329 | MS.gene94380:CDS |
| TCTGGCCAAGATAACATCTG+TGG | 0.625993 | 4.3:+67041324 | MS.gene94380:CDS |
| GCACTTGAAAGAGATAGCAG+AGG | 0.626453 | 4.3:+67038692 | MS.gene94380:CDS |
| ACACAGCCAGCAACAACTAG+TGG | 0.634478 | 4.3:-67037703 | None:intergenic |
| TGGCTGAGCAGTGAAGATAC+CGG | 0.637316 | 4.3:+67038360 | MS.gene94380:CDS |
| GCCAGCGCTTGACCTTCCAA+AGG | 0.638812 | 4.3:+67037861 | MS.gene94380:CDS |
| ATGAATCGAGAATATACTGT+GGG | 0.644518 | 4.3:+67038865 | MS.gene94380:CDS |
| ATCCATGGCAGATTGACTTG+GGG | 0.656976 | 4.3:-67037647 | None:intergenic |
| AACGGAGAACGGAATCCCGG+TGG | 0.670316 | 4.3:-67034706 | None:intergenic |
| CCGACGGTAACGGAGTTCAA+CGG | 0.671119 | 4.3:-67034724 | None:intergenic |
| CCTTAACTGAGCTTGTGCCG+GGG | 0.671188 | 4.3:+67038911 | MS.gene94380:CDS |
| TGCAAGACAAAACATGCTCG+AGG | 0.679994 | 4.3:+67038072 | MS.gene94380:CDS |
| GGTTGGGCGTGTAAAATCTG+TGG | 0.710077 | 4.3:+67038314 | MS.gene94380:intron |
| AATGAATCGAGAATATACTG+TGG | 0.719912 | 4.3:+67038864 | MS.gene94380:CDS |
| AAAATGAATAGGGACACCTG+CGG | 0.776532 | 4.3:+67040429 | MS.gene94380:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 67034661 | 67041807 | 67034661 | ID=MS.gene94380 |
| chr4.3 | mRNA | 67034661 | 67041807 | 67034661 | ID=MS.gene94380.t1;Parent=MS.gene94380 |
| chr4.3 | exon | 67034661 | 67034747 | 67034661 | ID=MS.gene94380.t1.exon1;Parent=MS.gene94380.t1 |
| chr4.3 | CDS | 67034661 | 67034747 | 67034661 | ID=cds.MS.gene94380.t1;Parent=MS.gene94380.t1 |
| chr4.3 | exon | 67034899 | 67034937 | 67034899 | ID=MS.gene94380.t1.exon2;Parent=MS.gene94380.t1 |
| chr4.3 | CDS | 67034899 | 67034937 | 67034899 | ID=cds.MS.gene94380.t1;Parent=MS.gene94380.t1 |
| chr4.3 | exon | 67035054 | 67035145 | 67035054 | ID=MS.gene94380.t1.exon3;Parent=MS.gene94380.t1 |
| chr4.3 | CDS | 67035054 | 67035145 | 67035054 | ID=cds.MS.gene94380.t1;Parent=MS.gene94380.t1 |
| chr4.3 | exon | 67037633 | 67037882 | 67037633 | ID=MS.gene94380.t1.exon4;Parent=MS.gene94380.t1 |
| chr4.3 | CDS | 67037633 | 67037882 | 67037633 | ID=cds.MS.gene94380.t1;Parent=MS.gene94380.t1 |
| chr4.3 | exon | 67038006 | 67038125 | 67038006 | ID=MS.gene94380.t1.exon5;Parent=MS.gene94380.t1 |
| chr4.3 | CDS | 67038006 | 67038125 | 67038006 | ID=cds.MS.gene94380.t1;Parent=MS.gene94380.t1 |
| chr4.3 | exon | 67038315 | 67038390 | 67038315 | ID=MS.gene94380.t1.exon6;Parent=MS.gene94380.t1 |
| chr4.3 | CDS | 67038315 | 67038390 | 67038315 | ID=cds.MS.gene94380.t1;Parent=MS.gene94380.t1 |
| chr4.3 | exon | 67038577 | 67038785 | 67038577 | ID=MS.gene94380.t1.exon7;Parent=MS.gene94380.t1 |
| chr4.3 | CDS | 67038577 | 67038785 | 67038577 | ID=cds.MS.gene94380.t1;Parent=MS.gene94380.t1 |
| chr4.3 | exon | 67038862 | 67038970 | 67038862 | ID=MS.gene94380.t1.exon8;Parent=MS.gene94380.t1 |
| chr4.3 | CDS | 67038862 | 67038970 | 67038862 | ID=cds.MS.gene94380.t1;Parent=MS.gene94380.t1 |
| chr4.3 | exon | 67039341 | 67039433 | 67039341 | ID=MS.gene94380.t1.exon9;Parent=MS.gene94380.t1 |
| chr4.3 | CDS | 67039341 | 67039433 | 67039341 | ID=cds.MS.gene94380.t1;Parent=MS.gene94380.t1 |
| chr4.3 | exon | 67040440 | 67040600 | 67040440 | ID=MS.gene94380.t1.exon10;Parent=MS.gene94380.t1 |
| chr4.3 | CDS | 67040440 | 67040600 | 67040440 | ID=cds.MS.gene94380.t1;Parent=MS.gene94380.t1 |
| chr4.3 | exon | 67041256 | 67041807 | 67041256 | ID=MS.gene94380.t1.exon11;Parent=MS.gene94380.t1 |
| chr4.3 | CDS | 67041256 | 67041807 | 67041256 | ID=cds.MS.gene94380.t1;Parent=MS.gene94380.t1 |
| Gene Sequence |
| Protein sequence |
