Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94960.t1 | XP_024635441.1 | 94.6 | 688 | 34 | 3 | 1 | 686 | 1 | 687 | 0.00E+00 | 1265 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94960.t1 | Q9LG26 | 57.4 | 660 | 272 | 2 | 22 | 681 | 21 | 671 | 2.0e-214 | 746.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94960.t1 | A0A396ISR3 | 94.6 | 688 | 34 | 3 | 1 | 686 | 1 | 687 | 0.0e+00 | 1265.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene94960 | MS.gene21710 | PPI |
| MS.gene94960 | MS.gene21912 | PPI |
| MS.gene94960 | MS.gene91519 | PPI |
| MS.gene94960 | MS.gene058147 | PPI |
| MS.gene94960 | MS.gene024734 | PPI |
| MS.gene94960 | MS.gene021698 | PPI |
| MS.gene94960 | MS.gene043239 | PPI |
| MS.gene79599 | MS.gene94960 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94960.t1 | MTR_3g460830 | 97.510 | 241 | 5 | 1 | 447 | 686 | 5 | 245 | 1.32e-171 | 490 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94960.t1 | AT1G55870 | 59.646 | 622 | 242 | 2 | 37 | 658 | 36 | 648 | 0.0 | 755 |
| MS.gene94960.t1 | AT1G55870 | 55.819 | 464 | 198 | 1 | 195 | 658 | 8 | 464 | 1.07e-179 | 521 |
| MS.gene94960.t1 | AT1G55870 | 60.870 | 345 | 128 | 1 | 314 | 658 | 1 | 338 | 6.05e-145 | 427 |
| MS.gene94960.t1 | AT1G55870 | 60.870 | 345 | 128 | 1 | 314 | 658 | 1 | 338 | 6.05e-145 | 427 |
| MS.gene94960.t1 | AT3G25430 | 27.613 | 641 | 369 | 21 | 18 | 635 | 4 | 572 | 9.63e-59 | 209 |
| MS.gene94960.t1 | AT3G25430 | 27.201 | 636 | 361 | 20 | 18 | 635 | 4 | 555 | 6.88e-56 | 201 |
Find 134 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTCAGGAGCTTTCTGGTTT+TGG | 0.164511 | 3.1:-44718154 | MS.gene94960:CDS |
| TTTCCACGTCAGGAGCTTTC+TGG | 0.182890 | 3.1:-44718160 | MS.gene94960:CDS |
| TTCCTTGTATTCAATCAATT+TGG | 0.228163 | 3.1:+44712738 | None:intergenic |
| TTGAGAACATTGTTATTATC+TGG | 0.233378 | 3.1:-44713092 | MS.gene94960:CDS |
| CTCCTAAATCACTGCATAAC+TGG | 0.233696 | 3.1:+44713295 | None:intergenic |
| GCCCAGTTATGCAGTGATTT+AGG | 0.257302 | 3.1:-44713297 | MS.gene94960:CDS |
| TGCTTCCCTTTCTTGTTCTC+TGG | 0.265913 | 3.1:+44717643 | None:intergenic |
| GACTACTTCGATGGATTTCT+TGG | 0.268029 | 3.1:-44718107 | MS.gene94960:CDS |
| TGTAACTAGCTCGGTGCTTT+TGG | 0.283680 | 3.1:+44716308 | None:intergenic |
| CTCGTACTTCACTTGCCTTC+AGG | 0.295775 | 3.1:+44713055 | None:intergenic |
| GCATCCGCTGGGAAAACTTT+TGG | 0.305585 | 3.1:-44712875 | MS.gene94960:CDS |
| AGAAACTCTGTTTGCTGATC+AGG | 0.312532 | 3.1:-44712788 | MS.gene94960:CDS |
| TCACTTGCCTTCAGGTTAGA+AGG | 0.335224 | 3.1:+44713063 | None:intergenic |
| TTTACATTCTCTTCTATAGT+TGG | 0.344960 | 3.1:+44712691 | None:intergenic |
| CATCCAGCTCTCAAGCTTGA+TGG | 0.345888 | 3.1:-44717389 | MS.gene94960:CDS |
| AATGTGAACAATGAGGTTTC+TGG | 0.349688 | 3.1:-44717114 | MS.gene94960:CDS |
| AACAAGAAAGGGAAGCAATA+AGG | 0.360923 | 3.1:-44717637 | MS.gene94960:CDS |
| TTGTGGCGGTTGAAGAGGTT+AGG | 0.365222 | 3.1:+44719628 | None:intergenic |
| AGTTTGATCGCTCCGATGTT+CGG | 0.382519 | 3.1:-44719437 | MS.gene94960:CDS |
| CATGTCTACAGCAAATTTAT+CGG | 0.389497 | 3.1:-44716507 | MS.gene94960:CDS |
| CTGTATACACAACTATTTGT+TGG | 0.390531 | 3.1:+44717088 | None:intergenic |
| AAGTTGAAGTTGATGATTCA+AGG | 0.401865 | 3.1:-44716266 | MS.gene94960:intron |
| ATGGCGTGATGGGTTATTAC+AGG | 0.412013 | 3.1:-44717489 | MS.gene94960:CDS |
| TTTCTAAATTAAATGATGTC+AGG | 0.412993 | 3.1:-44717577 | MS.gene94960:CDS |
| GGATGAACTAAACTTACTTC+TGG | 0.413972 | 3.1:-44717058 | MS.gene94960:intron |
| TGTTCTCAAACAAAATTTGT+TGG | 0.415338 | 3.1:+44713106 | None:intergenic |
| TGAGAACATTGTTATTATCT+GGG | 0.418628 | 3.1:-44713091 | MS.gene94960:CDS |
| AGCGGATGCAAAACCGAGAT+TGG | 0.425664 | 3.1:+44712889 | None:intergenic |
| GAACATCGGAGCGATCAAAC+TGG | 0.442573 | 3.1:+44719439 | None:intergenic |
| GGTTATTACAGGAGCAAAAT+CGG | 0.443983 | 3.1:-44717478 | MS.gene94960:CDS |
| TGTATACACAACTATTTGTT+GGG | 0.447606 | 3.1:+44717089 | None:intergenic |
| CCTGCCTCATGTTTGCCTCC+AGG | 0.448342 | 3.1:+44713351 | None:intergenic |
| TCGGCGTTTGTCCTTTCCGA+TGG | 0.449616 | 3.1:-44719368 | MS.gene94960:CDS |
| TGCATGCATTAAAATCAAAC+TGG | 0.450220 | 3.1:+44718077 | None:intergenic |
| AGGTTCGCCGTTGTTCAGTT+CGG | 0.453497 | 3.1:-44719387 | MS.gene94960:CDS |
| TGTCTGAACACTCACTATCA+TGG | 0.455862 | 3.1:+44717600 | None:intergenic |
| TTGGAGCCAGCAGCAATTTG+TGG | 0.460161 | 3.1:+44716327 | None:intergenic |
| AACAGTCGTTAGCGGAGCTC+CGG | 0.462053 | 3.1:-44719542 | MS.gene94960:CDS |
| ATTTATCCAGAGAACAAGAA+AGG | 0.462100 | 3.1:-44717649 | MS.gene94960:CDS |
| CGGAGCTCCGGCACCATGTC+CGG | 0.464535 | 3.1:-44719530 | MS.gene94960:CDS |
| TGTGGCGGTTGAAGAGGTTA+GGG | 0.473888 | 3.1:+44719629 | None:intergenic |
| ACGGATATGCGACGAAGGAC+TGG | 0.477094 | 3.1:+44719334 | None:intergenic |
| AGAAATCCATCGAAGTAGTC+TGG | 0.482313 | 3.1:+44718110 | None:intergenic |
| TGTAGTTTCTGTCTACCACT+TGG | 0.491252 | 3.1:-44713001 | MS.gene94960:CDS |
| GCAAACAGAGTTTCTGATAA+GGG | 0.491406 | 3.1:+44712796 | None:intergenic |
| CGGCGTTTGTCCTTTCCGAT+GGG | 0.493200 | 3.1:-44719367 | MS.gene94960:CDS |
| CTTGTAGAAGGAGGTAAAAG+AGG | 0.494009 | 3.1:-44716731 | MS.gene94960:intron |
| CTGAACAACGGCGAACCTAC+TGG | 0.497338 | 3.1:+44719392 | None:intergenic |
| AAACTTCGAACAGTCGTTAG+CGG | 0.497757 | 3.1:-44719550 | MS.gene94960:CDS |
| TCAGAACAGAAGTTGATAGT+TGG | 0.502195 | 3.1:-44716637 | MS.gene94960:CDS |
| TATATCTTAGTTATATGCAT+GGG | 0.502273 | 3.1:-44713200 | MS.gene94960:CDS |
| GGATATGATGCTTTCATGAC+TGG | 0.504444 | 3.1:-44713330 | MS.gene94960:CDS |
| AGCAAACAGAGTTTCTGATA+AGG | 0.504846 | 3.1:+44712795 | None:intergenic |
| GAAAGCTTAGAGAGAAGTAA+TGG | 0.511591 | 3.1:-44712913 | MS.gene94960:CDS |
| TGGCAGAGAAATTCATTACA+TGG | 0.515487 | 3.1:+44718130 | None:intergenic |
| TCAGCTTAAGTTGATTCAGT+TGG | 0.517220 | 3.1:-44717354 | MS.gene94960:intron |
| AACCAAATTGATTGAATACA+AGG | 0.519448 | 3.1:-44712740 | MS.gene94960:CDS |
| AGATCGTCCAGCTGGAATCC+TGG | 0.520249 | 3.1:-44713369 | MS.gene94960:CDS |
| AGGTAAAAGAGGAGAATCAA+AGG | 0.522105 | 3.1:-44716720 | MS.gene94960:CDS |
| GGCGAAATCCAACTGCAGCT+TGG | 0.522198 | 3.1:+44716680 | None:intergenic |
| TCCTAAATCACTGCATAACT+GGG | 0.523426 | 3.1:+44713296 | None:intergenic |
| TTTATCCAGAGAACAAGAAA+GGG | 0.523900 | 3.1:-44717648 | MS.gene94960:CDS |
| TTCTAAATTAAATGATGTCA+GGG | 0.525924 | 3.1:-44717576 | MS.gene94960:CDS |
| TTCGAAGTTTGCAACGGTGA+CGG | 0.527957 | 3.1:+44719563 | None:intergenic |
| CGAAGTCCGACGACCGGACA+TGG | 0.528371 | 3.1:+44719517 | None:intergenic |
| TGAAAGATAAGTTCAGTGAA+TGG | 0.529459 | 3.1:-44717508 | MS.gene94960:CDS |
| GCTGATCAGGCAAAGGCAGT+TGG | 0.534922 | 3.1:-44712775 | MS.gene94960:CDS |
| GCAACGGTGACGGTCTTCGA+TGG | 0.535587 | 3.1:+44719573 | None:intergenic |
| AATCGGTCAGACCAAATTCA+AGG | 0.539111 | 3.1:-44717461 | MS.gene94960:CDS |
| TGGCGACGAAGTCCGACGAC+CGG | 0.544719 | 3.1:+44719511 | None:intergenic |
| GTCTGAACACTCACTATCAT+GGG | 0.545278 | 3.1:+44717601 | None:intergenic |
| GTATTTGCAGATCGTCCAGC+TGG | 0.545385 | 3.1:-44713377 | MS.gene94960:intron |
| AAGTTCAGTGAATGGCGTGA+TGG | 0.547765 | 3.1:-44717500 | MS.gene94960:CDS |
| CTATATCTTAGTTATATGCA+TGG | 0.549824 | 3.1:-44713201 | MS.gene94960:CDS |
| AGTTCAGTGAATGGCGTGAT+GGG | 0.550536 | 3.1:-44717499 | MS.gene94960:CDS |
| TTCCTTCAACAAAAGAAAGT+CGG | 0.553574 | 3.1:+44712933 | None:intergenic |
| AAACTGGAATGAATCACGCC+AGG | 0.554543 | 3.1:+44719455 | None:intergenic |
| CGGTGGTGTGGTTGTTGTGG+CGG | 0.555354 | 3.1:+44719614 | None:intergenic |
| GGTTGTTGTGGCGGTTGAAG+AGG | 0.557395 | 3.1:+44719623 | None:intergenic |
| GATATTATAGATCTAAATAC+CGG | 0.557851 | 3.1:-44713177 | MS.gene94960:CDS |
| GGGATTTCCTTCTAACCTGA+AGG | 0.558626 | 3.1:-44713070 | MS.gene94960:CDS |
| CAAAGGCAGTTGGCATGAAG+TGG | 0.559051 | 3.1:-44712765 | MS.gene94960:CDS |
| TCAACTTCTGTTCTGATGAA+AGG | 0.559958 | 3.1:+44716644 | None:intergenic |
| GAGAAGTAATGGTCCAATCT+CGG | 0.560192 | 3.1:-44712902 | MS.gene94960:CDS |
| CCTGGAGGCAAACATGAGGC+AGG | 0.561089 | 3.1:-44713351 | MS.gene94960:CDS |
| CGTGATAGTGTCGAAGAAGA+AGG | 0.563909 | 3.1:+44719666 | None:intergenic |
| ATGAAGATCCAAGCTGCAGT+TGG | 0.563917 | 3.1:-44716688 | MS.gene94960:CDS |
| AATCCATCAAGCTTGAGAGC+TGG | 0.565036 | 3.1:+44717386 | None:intergenic |
| TTTGCCTCCAGGATTCCAGC+TGG | 0.565341 | 3.1:+44713362 | None:intergenic |
| GCCATCGACCTCGAGATGAC+CGG | 0.568536 | 3.1:-44719492 | MS.gene94960:CDS |
| TTGCGGTGGTGTGGTTGTTG+TGG | 0.569522 | 3.1:+44719611 | None:intergenic |
| TTTAATGCATGCATACATGA+AGG | 0.569755 | 3.1:-44718067 | MS.gene94960:intron |
| TCTCTGCCAGACTACTTCGA+TGG | 0.569944 | 3.1:-44718116 | MS.gene94960:CDS |
| CCGACGACCGGACATGGTGC+CGG | 0.574272 | 3.1:+44719523 | None:intergenic |
| CGTCTCTTGAGGTTATAGCT+TGG | 0.582065 | 3.1:+44713132 | None:intergenic |
| GAGAACATTGTTATTATCTG+GGG | 0.588898 | 3.1:-44713090 | MS.gene94960:CDS |
| CTCCGATGTTCGGTATCTCA+AGG | 0.590111 | 3.1:-44719427 | MS.gene94960:CDS |
| GGTAAAAGAGGAGAATCAAA+GGG | 0.595963 | 3.1:-44716719 | MS.gene94960:CDS |
| GCCGGTCATCTCGAGGTCGA+TGG | 0.597994 | 3.1:+44719491 | None:intergenic |
| AGGTTAGAGACTCCGCCAGT+AGG | 0.598496 | 3.1:-44719407 | MS.gene94960:CDS |
| TTGGGAAGGTGTAACTAGCT+CGG | 0.601643 | 3.1:+44716299 | None:intergenic |
| TCTGTTTGCTGATCAGGCAA+AGG | 0.603663 | 3.1:-44712782 | MS.gene94960:CDS |
| ATGTCTGCCATGTTGACAGA+AGG | 0.603946 | 3.1:+44717548 | None:intergenic |
| TTATATCAATGTGAACAATG+AGG | 0.605285 | 3.1:-44717121 | MS.gene94960:CDS |
| TTGTAACGCCGGTCATCTCG+AGG | 0.610599 | 3.1:+44719484 | None:intergenic |
| CCGGCACCATGTCCGGTCGT+CGG | 0.612633 | 3.1:-44719523 | MS.gene94960:CDS |
| AAACCAGAAAGCTCCTGACG+TGG | 0.613387 | 3.1:+44718157 | None:intergenic |
| TTTGTTGGTACCGTCTCTTG+AGG | 0.616510 | 3.1:+44713121 | None:intergenic |
| CCGGCGTTACAAGTTCTCCC+TGG | 0.620249 | 3.1:-44719473 | MS.gene94960:CDS |
| TGATTCACATTGCGGTGGTG+TGG | 0.624689 | 3.1:+44719602 | None:intergenic |
| GGCAGAGAAATTCATTACAT+GGG | 0.625343 | 3.1:+44718131 | None:intergenic |
| GGACATACCTTCTGTCAACA+TGG | 0.630760 | 3.1:-44717555 | MS.gene94960:CDS |
| AACTGGAATGAATCACGCCA+GGG | 0.631908 | 3.1:+44719456 | None:intergenic |
| GAATCCTGGAGGCAAACATG+AGG | 0.644653 | 3.1:-44713355 | MS.gene94960:CDS |
| AATAGTTGTGTATACAGACT+CGG | 0.645056 | 3.1:-44717082 | MS.gene94960:CDS |
| ATATCTTAGTTATATGCATG+GGG | 0.647653 | 3.1:-44713199 | MS.gene94960:CDS |
| ACTGGTTAGACGAATCCCAT+CGG | 0.648981 | 3.1:+44719352 | None:intergenic |
| ACACTGCTGTTGCATCCAAG+TGG | 0.655012 | 3.1:+44712986 | None:intergenic |
| CCAGGGAGAACTTGTAACGC+CGG | 0.663946 | 3.1:+44719473 | None:intergenic |
| ACAAACGCCGAACTGAACAA+CGG | 0.666707 | 3.1:+44719380 | None:intergenic |
| AACCTTGAGATACCGAACAT+CGG | 0.667246 | 3.1:+44719425 | None:intergenic |
| ATGAAAGGAGATCAATAACA+TGG | 0.667767 | 3.1:+44716659 | None:intergenic |
| CGACTGTTCGAAGTTTGCAA+CGG | 0.674225 | 3.1:+44719557 | None:intergenic |
| GTGTTACGGATATGCGACGA+AGG | 0.674644 | 3.1:+44719329 | None:intergenic |
| CAAGCTATAACCTCAAGAGA+CGG | 0.676408 | 3.1:-44713131 | MS.gene94960:CDS |
| GGAAACGCTGATTCACATTG+CGG | 0.692132 | 3.1:+44719594 | None:intergenic |
| AACGCTGATTCACATTGCGG+TGG | 0.693300 | 3.1:+44719597 | None:intergenic |
| TGGAAAGCACTTGTTAACAG+AGG | 0.695071 | 3.1:+44716452 | None:intergenic |
| TTAGACGAATCCCATCGGAA+AGG | 0.705477 | 3.1:+44719357 | None:intergenic |
| ACAAAACATAGAAAATGCAG+AGG | 0.706266 | 3.1:+44716350 | None:intergenic |
| AACAACGGCGAACCTACTGG+CGG | 0.710795 | 3.1:+44719395 | None:intergenic |
| AATCAACTTAAGCTGACGTG+AGG | 0.725978 | 3.1:+44717361 | None:intergenic |
| TCGTCCAGCTGGAATCCTGG+AGG | 0.728618 | 3.1:-44713366 | MS.gene94960:CDS |
| AGTTGTGTATACAGACTCGG+AGG | 0.742262 | 3.1:-44717079 | MS.gene94960:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 44712669 | 44719706 | 44712669 | ID=MS.gene94960 |
| chr3.1 | mRNA | 44712669 | 44719706 | 44712669 | ID=MS.gene94960.t1;Parent=MS.gene94960 |
| chr3.1 | exon | 44719336 | 44719706 | 44719336 | ID=MS.gene94960.t1.exon1;Parent=MS.gene94960.t1 |
| chr3.1 | CDS | 44719336 | 44719706 | 44719336 | ID=cds.MS.gene94960.t1;Parent=MS.gene94960.t1 |
| chr3.1 | exon | 44718068 | 44718198 | 44718068 | ID=MS.gene94960.t1.exon2;Parent=MS.gene94960.t1 |
| chr3.1 | CDS | 44718068 | 44718198 | 44718068 | ID=cds.MS.gene94960.t1;Parent=MS.gene94960.t1 |
| chr3.1 | exon | 44717355 | 44717680 | 44717355 | ID=MS.gene94960.t1.exon3;Parent=MS.gene94960.t1 |
| chr3.1 | CDS | 44717355 | 44717680 | 44717355 | ID=cds.MS.gene94960.t1;Parent=MS.gene94960.t1 |
| chr3.1 | exon | 44717059 | 44717172 | 44717059 | ID=MS.gene94960.t1.exon4;Parent=MS.gene94960.t1 |
| chr3.1 | CDS | 44717059 | 44717172 | 44717059 | ID=cds.MS.gene94960.t1;Parent=MS.gene94960.t1 |
| chr3.1 | exon | 44716620 | 44716746 | 44716620 | ID=MS.gene94960.t1.exon5;Parent=MS.gene94960.t1 |
| chr3.1 | CDS | 44716620 | 44716746 | 44716620 | ID=cds.MS.gene94960.t1;Parent=MS.gene94960.t1 |
| chr3.1 | exon | 44716267 | 44716537 | 44716267 | ID=MS.gene94960.t1.exon6;Parent=MS.gene94960.t1 |
| chr3.1 | CDS | 44716267 | 44716537 | 44716267 | ID=cds.MS.gene94960.t1;Parent=MS.gene94960.t1 |
| chr3.1 | exon | 44712669 | 44713389 | 44712669 | ID=MS.gene94960.t1.exon7;Parent=MS.gene94960.t1 |
| chr3.1 | CDS | 44712669 | 44713389 | 44712669 | ID=cds.MS.gene94960.t1;Parent=MS.gene94960.t1 |
| Gene Sequence |
| Protein sequence |
