Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95252.t1 | XP_013445072.1 | 99.8 | 464 | 1 | 0 | 7 | 470 | 34 | 497 | 6.20E-275 | 956.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95252.t1 | Q6DKP5 | 37.0 | 330 | 198 | 7 | 141 | 463 | 158 | 484 | 4.7e-53 | 210.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95252.t1 | A0A072TQH3 | 99.8 | 464 | 1 | 0 | 7 | 470 | 34 | 497 | 4.5e-275 | 956.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene95252 | MS.gene70587 | PPI |
| MS.gene95252 | MS.gene87680 | PPI |
| MS.gene92768 | MS.gene95252 | PPI |
| MS.gene036937 | MS.gene95252 | PPI |
| MS.gene022257 | MS.gene95252 | PPI |
| MS.gene25914 | MS.gene95252 | PPI |
| MS.gene51850 | MS.gene95252 | PPI |
| MS.gene27507 | MS.gene95252 | PPI |
| MS.gene95252 | MS.gene074392 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95252.t1 | MTR_8g037340 | 99.785 | 466 | 1 | 0 | 5 | 470 | 32 | 497 | 0.0 | 973 |
| MS.gene95252.t1 | MTR_8g037340 | 93.750 | 496 | 1 | 1 | 5 | 470 | 32 | 527 | 0.0 | 958 |
| MS.gene95252.t1 | MTR_1g072140 | 28.182 | 330 | 205 | 13 | 139 | 462 | 12 | 315 | 1.51e-25 | 107 |
| MS.gene95252.t1 | MTR_4g119620 | 25.915 | 328 | 196 | 14 | 154 | 463 | 27 | 325 | 5.72e-19 | 88.2 |
| MS.gene95252.t1 | MTR_4g123597 | 21.557 | 334 | 227 | 8 | 154 | 459 | 106 | 432 | 3.77e-12 | 69.3 |
| MS.gene95252.t1 | MTR_2g079740 | 22.887 | 284 | 183 | 8 | 181 | 446 | 474 | 739 | 6.46e-11 | 65.1 |
| MS.gene95252.t1 | MTR_8g092890 | 21.849 | 238 | 167 | 6 | 181 | 417 | 276 | 495 | 6.47e-11 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95252.t1 | AT2G16405 | 75.108 | 462 | 110 | 4 | 5 | 465 | 24 | 481 | 0.0 | 725 |
| MS.gene95252.t1 | AT3G49660 | 27.329 | 322 | 189 | 16 | 156 | 463 | 26 | 316 | 2.06e-21 | 95.1 |
| MS.gene95252.t1 | AT4G02730 | 25.208 | 361 | 229 | 15 | 110 | 462 | 5 | 332 | 2.86e-17 | 83.2 |
| MS.gene95252.t1 | AT5G54200 | 25.207 | 242 | 153 | 9 | 181 | 416 | 453 | 672 | 4.15e-12 | 68.9 |
| MS.gene95252.t1 | AT5G53500 | 24.473 | 237 | 158 | 7 | 181 | 415 | 313 | 530 | 4.17e-12 | 68.9 |
| MS.gene95252.t1 | AT5G53500 | 24.473 | 237 | 158 | 7 | 181 | 415 | 313 | 530 | 4.17e-12 | 68.9 |
Find 107 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCCTTCTTTCTCTTGAAAA+AGG | 0.184205 | 8.4:-60605941 | MS.gene95252:CDS |
| GAGGTGGACAGCTGGAGTTC+TGG | 0.218453 | 8.4:-60610809 | MS.gene95252:CDS |
| AGTTCTGACCTACTTGCTTA+TGG | 0.246804 | 8.4:-60609804 | MS.gene95252:CDS |
| TGGATAAAACTGTGAGAGTA+TGG | 0.287100 | 8.4:-60608245 | MS.gene95252:CDS |
| GTACATTGCTTCTGCTTCAT+TGG | 0.327705 | 8.4:-60608265 | MS.gene95252:CDS |
| AATTGCATGCGGGTGATATA+TGG | 0.338828 | 8.4:-60608207 | MS.gene95252:CDS |
| GTAGTTACGGTGATAAAGTT+TGG | 0.339409 | 8.4:-60609834 | MS.gene95252:intron |
| AGGTGGACAGCTGGAGTTCT+GGG | 0.349940 | 8.4:-60610808 | MS.gene95252:CDS |
| CCCGGAGTACTTCGAAGACT+TGG | 0.357960 | 8.4:+60611519 | None:intergenic |
| GGCATATTACAGTGTGGCTT+TGG | 0.361902 | 8.4:-60606041 | MS.gene95252:intron |
| ACATAAACAGGCCTAGGAAC+TGG | 0.364322 | 8.4:-60611560 | MS.gene95252:CDS |
| AGAGTATGGGAGATAGAAAA+AGG | 0.367149 | 8.4:-60608231 | MS.gene95252:CDS |
| TAGGCCTGTTTATGTAATTC+CGG | 0.374723 | 8.4:+60611568 | None:intergenic |
| TGACGCCGAAGTTACCTCTA+TGG | 0.376140 | 8.4:-60606456 | MS.gene95252:CDS |
| TCCAATCATAGAGCAAGTTT+TGG | 0.376757 | 8.4:-60610061 | MS.gene95252:CDS |
| TTTACGGCATAGTAATTGTT+TGG | 0.377298 | 8.4:-60605521 | MS.gene95252:CDS |
| AAATTCAGCAACCTGTTTCC+CGG | 0.380388 | 8.4:+60611501 | None:intergenic |
| GTTCTGACCTACTTGCTTAT+GGG | 0.394653 | 8.4:-60609803 | MS.gene95252:CDS |
| CAGTCACAACAGTGCAGTAT+AGG | 0.394700 | 8.4:-60606219 | MS.gene95252:CDS |
| CCTAGGAACTGGGAAAGTTT+AGG | 0.402238 | 8.4:-60611549 | MS.gene95252:CDS |
| TTGTTCACACATGTATGCTT+TGG | 0.408174 | 8.4:+60605690 | None:intergenic |
| TTCGAAGTACTCCGGGAAAC+AGG | 0.408718 | 8.4:-60611512 | MS.gene95252:intron |
| AATAACAACTTCCTTTCTGT+TGG | 0.412808 | 8.4:-60607203 | MS.gene95252:CDS |
| AGTGTGGCTTTGGAGATAAA+AGG | 0.415343 | 8.4:-60606031 | MS.gene95252:CDS |
| GCCAAAACTTGCTCTATGAT+TGG | 0.428070 | 8.4:+60610060 | None:intergenic |
| CCGGAGTACTTCGAAGACTT+GGG | 0.432049 | 8.4:+60611520 | None:intergenic |
| GGGTCATCGGTTTCCTGTGA+TGG | 0.438655 | 8.4:-60605589 | MS.gene95252:CDS |
| TAGTTACGGTGATAAAGTTT+GGG | 0.440842 | 8.4:-60609833 | MS.gene95252:CDS |
| AGAAGACAGCAGAATAAGTC+TGG | 0.447786 | 8.4:+60612628 | None:intergenic |
| TCTGATCGTCCACGAATTCG+TGG | 0.450477 | 8.4:-60610016 | MS.gene95252:CDS |
| GACCAACGGGAGAGTGGACT+TGG | 0.450591 | 8.4:+60610833 | None:intergenic |
| AAAATATGAGATGGCCAGTA+TGG | 0.452859 | 8.4:+60606426 | None:intergenic |
| ATGCATTCCTACGAAGGAAT+AGG | 0.453630 | 8.4:+60609982 | None:intergenic |
| CATACCGGAATTACATAAAC+AGG | 0.453670 | 8.4:-60611572 | MS.gene95252:CDS |
| CCTAAACTTTCCCAGTTCCT+AGG | 0.455108 | 8.4:+60611549 | None:intergenic |
| TTCCTGTGATGGGGATTGCT+TGG | 0.458213 | 8.4:-60605578 | MS.gene95252:CDS |
| GGTCATCGGTTTCCTGTGAT+GGG | 0.461880 | 8.4:-60605588 | MS.gene95252:CDS |
| AAGAAGGAGGCGACGAATGC+CGG | 0.463736 | 8.4:+60612570 | None:intergenic |
| GTTGGTCTGAGGTGGACAGC+TGG | 0.471148 | 8.4:-60610817 | MS.gene95252:CDS |
| TTGTACTCACAGGGTGGAAA+CGG | 0.473001 | 8.4:+60608163 | None:intergenic |
| TCTATGGACCACGACCATAC+TGG | 0.474694 | 8.4:-60606440 | MS.gene95252:CDS |
| GCTGTCCACCTCAGACCAAC+GGG | 0.477029 | 8.4:+60610820 | None:intergenic |
| TCAGTCATCAAACAATTAAA+CGG | 0.482319 | 8.4:-60609735 | MS.gene95252:CDS |
| TTCGACCAGTGCAAAGCCTC+TGG | 0.486329 | 8.4:-60609953 | MS.gene95252:intron |
| CGGGAGAGTGGACTTGGAGA+TGG | 0.503119 | 8.4:+60610839 | None:intergenic |
| TGGATACTGAATTTGCAACT+TGG | 0.513649 | 8.4:+60610080 | None:intergenic |
| CTATACTGCACTGTTGTGAC+TGG | 0.513927 | 8.4:+60606220 | None:intergenic |
| TTTAATTGTTTGATGACTGA+AGG | 0.515062 | 8.4:+60609738 | None:intergenic |
| CTTGCTTATGGGGCATCAGA+TGG | 0.516204 | 8.4:-60609792 | MS.gene95252:CDS |
| TAGATTTCCATCCTGAGTAC+AGG | 0.521762 | 8.4:+60606159 | None:intergenic |
| CCCAAGTCTTCGAAGTACTC+CGG | 0.525236 | 8.4:-60611520 | MS.gene95252:CDS |
| GGAAAACTTACGTGGCATAC+CGG | 0.525881 | 8.4:-60611587 | MS.gene95252:CDS |
| GAGCCTGCCTATTCCTTCGT+AGG | 0.527204 | 8.4:-60609989 | MS.gene95252:CDS |
| CGACGAATGCCGGTGTACTC+AGG | 0.531207 | 8.4:+60612580 | None:intergenic |
| ACTTCGATGACGATGTGAGC+GGG | 0.531444 | 8.4:+60606261 | None:intergenic |
| CATAAACAGGCCTAGGAACT+GGG | 0.532543 | 8.4:-60611559 | MS.gene95252:CDS |
| TGTTTGCATTTCCAACAGAA+AGG | 0.536794 | 8.4:+60607192 | None:intergenic |
| TTCTGACCTACTTGCTTATG+GGG | 0.543681 | 8.4:-60609802 | MS.gene95252:CDS |
| TACCTGTGATGTCTTTCGAA+TGG | 0.549760 | 8.4:+60609712 | None:intergenic |
| AGCTGGAATTCTCCGCCGTA+AGG | 0.553349 | 8.4:-60612533 | MS.gene95252:intron |
| TCCACTCTCCCGTTGGTCTG+AGG | 0.553452 | 8.4:-60610828 | MS.gene95252:CDS |
| CTCCAAGTCCACTCTCCCGT+TGG | 0.556019 | 8.4:-60610835 | MS.gene95252:CDS |
| ACTCTCCCGTTGGTCTGAGG+TGG | 0.557213 | 8.4:-60610825 | MS.gene95252:CDS |
| GGCTCAACACCACGAATTCG+TGG | 0.557690 | 8.4:+60610007 | None:intergenic |
| TGTGTTGTTGACCTGTACTC+AGG | 0.559101 | 8.4:-60606170 | MS.gene95252:CDS |
| AGCTGTCCACCTCAGACCAA+CGG | 0.563191 | 8.4:+60610819 | None:intergenic |
| TGAAGGATTGGAGATGCAAT+GGG | 0.565209 | 8.4:-60611608 | MS.gene95252:intron |
| CCAAGTCTTCGAAGTACTCC+GGG | 0.573735 | 8.4:-60611519 | MS.gene95252:CDS |
| CGGAGTACTTCGAAGACTTG+GGG | 0.573894 | 8.4:+60611521 | None:intergenic |
| TTCCAAGCAATCCCCATCAC+AGG | 0.577118 | 8.4:+60605576 | None:intergenic |
| TACATGTGTGAACAAGCTAC+AGG | 0.579845 | 8.4:-60605682 | MS.gene95252:intron |
| TTGAAGGATTGGAGATGCAA+TGG | 0.580876 | 8.4:-60611609 | MS.gene95252:intron |
| TACTACCAGAGGCTTTGCAC+TGG | 0.583440 | 8.4:+60609948 | None:intergenic |
| ATGGGGATTGCTTGGAACCA+TGG | 0.583684 | 8.4:-60605570 | MS.gene95252:CDS |
| TGAAACACTGCACACAGTCA+AGG | 0.586692 | 8.4:+60609767 | None:intergenic |
| GGATAAAACTGTGAGAGTAT+GGG | 0.586995 | 8.4:-60608244 | MS.gene95252:CDS |
| CTGATGCCCCATAAGCAAGT+AGG | 0.590315 | 8.4:+60609796 | None:intergenic |
| GGATCAGCGTCGGAGGTGAG+AGG | 0.592584 | 8.4:+60612601 | None:intergenic |
| TGAGATGGCCAGTATGGTCG+TGG | 0.593850 | 8.4:+60606432 | None:intergenic |
| ACCTCAGACCAACGGGAGAG+TGG | 0.599253 | 8.4:+60610827 | None:intergenic |
| GGACAGCTGGAGTTCTGGGA+GGG | 0.602771 | 8.4:-60610804 | MS.gene95252:intron |
| CATCTCCACAAAATATGAGA+TGG | 0.602782 | 8.4:+60606417 | None:intergenic |
| GCTCGCTCAAGTTAACTCCT+CGG | 0.603557 | 8.4:-60605991 | MS.gene95252:CDS |
| TCCTACACCATGGCGGAGCA+CGG | 0.604515 | 8.4:-60612739 | None:intergenic |
| GGAATTACATAAACAGGCCT+AGG | 0.605131 | 8.4:-60611566 | MS.gene95252:CDS |
| GATGCAATGCATTCCTACGA+AGG | 0.607558 | 8.4:+60609976 | None:intergenic |
| GCGGGATAATATACCTGTGT+GGG | 0.610158 | 8.4:+60606279 | None:intergenic |
| TGAGTACAGGTCAACAACAC+AGG | 0.611720 | 8.4:+60606172 | None:intergenic |
| ATTCCTACGAAGGAATAGGC+AGG | 0.617187 | 8.4:+60609986 | None:intergenic |
| GTCATCGGTTTCCTGTGATG+GGG | 0.617591 | 8.4:-60605587 | MS.gene95252:CDS |
| AGTATGGTCGTGGTCCATAG+AGG | 0.617688 | 8.4:+60606442 | None:intergenic |
| TACTTCGATGACGATGTGAG+CGG | 0.618776 | 8.4:+60606260 | None:intergenic |
| AGTTTCTCTCTGCTAGCTCG+AGG | 0.630037 | 8.4:-60606196 | MS.gene95252:CDS |
| GTTGATGCATCAGTGGCCGA+TGG | 0.633147 | 8.4:-60610865 | MS.gene95252:intron |
| GGTGTACTCAGGATCAGCGT+CGG | 0.640899 | 8.4:+60612591 | None:intergenic |
| GTGGTCCATAGAGGTAACTT+CGG | 0.643822 | 8.4:+60606451 | None:intergenic |
| TGGACAGCTGGAGTTCTGGG+AGG | 0.650084 | 8.4:-60610805 | MS.gene95252:CDS |
| TTGTTGACCTGTACTCAGGA+TGG | 0.657122 | 8.4:-60606166 | MS.gene95252:CDS |
| GTAAATATGAACTCCCACAC+AGG | 0.663031 | 8.4:-60606292 | MS.gene95252:CDS |
| GGCCATTCGAAAGACATCAC+AGG | 0.665122 | 8.4:-60609714 | MS.gene95252:intron |
| GTACTCAGGATCAGCGTCGG+AGG | 0.665749 | 8.4:+60612594 | None:intergenic |
| ATATGCAGTTGCTGGAAGTG+AGG | 0.669433 | 8.4:-60605745 | MS.gene95252:intron |
| GACTTGGAGATGGAAACCAT+CGG | 0.697706 | 8.4:+60610849 | None:intergenic |
| AGCGGGATAATATACCTGTG+TGG | 0.702369 | 8.4:+60606278 | None:intergenic |
| ATGCAATGGGAAAACTTACG+TGG | 0.711127 | 8.4:-60611595 | MS.gene95252:CDS |
| GACGCTGATCCTGAGTACAC+CGG | 0.736062 | 8.4:-60612589 | MS.gene95252:CDS |
| ATGCGGAGTATACCTTACGG+CGG | 0.798435 | 8.4:+60612521 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 60605485 | 60612752 | 60605485 | ID=MS.gene95252 |
| chr8.4 | mRNA | 60605485 | 60612752 | 60605485 | ID=MS.gene95252.t1;Parent=MS.gene95252 |
| chr8.4 | exon | 60612740 | 60612752 | 60612740 | ID=MS.gene95252.t1.exon1;Parent=MS.gene95252.t1 |
| chr8.4 | CDS | 60612740 | 60612752 | 60612740 | ID=cds.MS.gene95252.t1;Parent=MS.gene95252.t1 |
| chr8.4 | exon | 60612534 | 60612652 | 60612534 | ID=MS.gene95252.t1.exon2;Parent=MS.gene95252.t1 |
| chr8.4 | CDS | 60612534 | 60612652 | 60612534 | ID=cds.MS.gene95252.t1;Parent=MS.gene95252.t1 |
| chr8.4 | exon | 60611513 | 60611625 | 60611513 | ID=MS.gene95252.t1.exon3;Parent=MS.gene95252.t1 |
| chr8.4 | CDS | 60611513 | 60611625 | 60611513 | ID=cds.MS.gene95252.t1;Parent=MS.gene95252.t1 |
| chr8.4 | exon | 60610805 | 60610874 | 60610805 | ID=MS.gene95252.t1.exon4;Parent=MS.gene95252.t1 |
| chr8.4 | CDS | 60610805 | 60610874 | 60610805 | ID=cds.MS.gene95252.t1;Parent=MS.gene95252.t1 |
| chr8.4 | exon | 60609954 | 60610107 | 60609954 | ID=MS.gene95252.t1.exon5;Parent=MS.gene95252.t1 |
| chr8.4 | CDS | 60609954 | 60610107 | 60609954 | ID=cds.MS.gene95252.t1;Parent=MS.gene95252.t1 |
| chr8.4 | exon | 60609715 | 60609852 | 60609715 | ID=MS.gene95252.t1.exon6;Parent=MS.gene95252.t1 |
| chr8.4 | CDS | 60609715 | 60609852 | 60609715 | ID=cds.MS.gene95252.t1;Parent=MS.gene95252.t1 |
| chr8.4 | exon | 60608173 | 60608312 | 60608173 | ID=MS.gene95252.t1.exon7;Parent=MS.gene95252.t1 |
| chr8.4 | CDS | 60608173 | 60608312 | 60608173 | ID=cds.MS.gene95252.t1;Parent=MS.gene95252.t1 |
| chr8.4 | exon | 60607181 | 60607228 | 60607181 | ID=MS.gene95252.t1.exon8;Parent=MS.gene95252.t1 |
| chr8.4 | CDS | 60607181 | 60607228 | 60607181 | ID=cds.MS.gene95252.t1;Parent=MS.gene95252.t1 |
| chr8.4 | exon | 60606412 | 60606522 | 60606412 | ID=MS.gene95252.t1.exon9;Parent=MS.gene95252.t1 |
| chr8.4 | CDS | 60606412 | 60606522 | 60606412 | ID=cds.MS.gene95252.t1;Parent=MS.gene95252.t1 |
| chr8.4 | exon | 60606148 | 60606329 | 60606148 | ID=MS.gene95252.t1.exon10;Parent=MS.gene95252.t1 |
| chr8.4 | CDS | 60606148 | 60606329 | 60606148 | ID=cds.MS.gene95252.t1;Parent=MS.gene95252.t1 |
| chr8.4 | exon | 60605930 | 60606051 | 60605930 | ID=MS.gene95252.t1.exon11;Parent=MS.gene95252.t1 |
| chr8.4 | CDS | 60605930 | 60606051 | 60605930 | ID=cds.MS.gene95252.t1;Parent=MS.gene95252.t1 |
| chr8.4 | exon | 60605683 | 60605759 | 60605683 | ID=MS.gene95252.t1.exon12;Parent=MS.gene95252.t1 |
| chr8.4 | CDS | 60605683 | 60605759 | 60605683 | ID=cds.MS.gene95252.t1;Parent=MS.gene95252.t1 |
| chr8.4 | exon | 60605485 | 60605610 | 60605485 | ID=MS.gene95252.t1.exon13;Parent=MS.gene95252.t1 |
| chr8.4 | CDS | 60605485 | 60605610 | 60605485 | ID=cds.MS.gene95252.t1;Parent=MS.gene95252.t1 |
| Gene Sequence |
| Protein sequence |
