AlfalfaGEDB | MS.gene96184

M. sativa cultivar XinJiangDaYe / MS.gene96184

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96184.t1	AES64280.1	92.2	129	9	1	1	128	1	129	1.50E-61	245.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96184.t1	G7IFK5	92.2	129	9	1	1	128	1	129	1.1e-61	245.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049821	MS.gene96184	0.802042	6.77E-49	-1.69E-46
MS.gene049825	MS.gene96184	0.810044	1.40E-50	-1.69E-46
MS.gene049873	MS.gene96184	0.806219	9.15E-50	-1.69E-46
MS.gene050303	MS.gene96184	0.842551	2.41E-58	-1.69E-46
MS.gene051700	MS.gene96184	0.804293	2.32E-49	-1.69E-46
MS.gene053226	MS.gene96184	0.803712	3.06E-49	-1.69E-46
MS.gene055808	MS.gene96184	0.823946	1.05E-53	-1.69E-46
MS.gene056091	MS.gene96184	0.803491	3.40E-49	-1.69E-46
MS.gene057060	MS.gene96184	0.813119	3.01E-51	-1.69E-46
MS.gene05954	MS.gene96184	0.853366	2.51E-61	-1.69E-46
MS.gene05979	MS.gene96184	0.808643	2.80E-50	-1.69E-46
MS.gene060429	MS.gene96184	0.85868	6.98E-63	-1.69E-46
MS.gene061040	MS.gene96184	-0.803245	3.82E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96184.t1	MTR_2g020550	92.248	129	9	1	1	128	1	129	4.28e-81	234
MS.gene96184.t1	MTR_2g020580	80.435	138	17	3	1	128	1	138	2.25e-57	174

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 29 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAATGGTTAATGTTAATT+TGG	0.155770	2.2:+64640894	MS.gene96184:CDS
GATTCTGAAGGTGACCTTAT+TGG	0.270756	2.2:+64640640	MS.gene96184:CDS
AAGGAAGATGAGAATGATAA+AGG	0.271877	2.2:+64640766	MS.gene96184:CDS
TCATAAAGAAATGAATGATA+TGG	0.276443	2.2:+64640576	None:intergenic
ATGTTGATGATAAACTATTT+TGG	0.278652	2.2:+64640920	MS.gene96184:CDS
TACCCTGCTGCTAATTACTT+TGG	0.359178	2.2:+64640676	MS.gene96184:CDS
TTCATCTTTGTTGCTTATTG+AGG	0.383420	2.2:+64640609	MS.gene96184:CDS
ATAGAGTAAAGAAGCCAATA+AGG	0.396823	2.2:-64640654	None:intergenic
ACCCTGCTGCTAATTACTTT+GGG	0.397321	2.2:+64640677	MS.gene96184:CDS
TGGGAGATATGTATGGAAGT+AGG	0.434821	2.2:+64640940	MS.gene96184:CDS
GGGAGATATGTATGGAAGTA+GGG	0.453186	2.2:+64640941	MS.gene96184:CDS
TGGTATGTGTAGCTTAGTTA+AGG	0.466313	2.2:+64640747	MS.gene96184:CDS
AGGAAGATGAGAATGATAAA+GGG	0.474639	2.2:+64640767	MS.gene96184:CDS
GATGAGAATGATAAAGGGTC+AGG	0.478591	2.2:+64640772	MS.gene96184:CDS
TGCTAATTACTTTGGGCACA+AGG	0.479009	2.2:+64640684	MS.gene96184:CDS
TGCCCAAAGTAATTAGCAGC+AGG	0.488758	2.2:-64640679	None:intergenic
TCCTCATCATCAACAAGTAA+AGG	0.503410	2.2:-64640865	None:intergenic
ACCTTTACTTGTTGATGATG+AGG	0.506285	2.2:+64640864	MS.gene96184:CDS
ACCATCATTGTCACCCTCAC+AGG	0.527345	2.2:-64640728	None:intergenic
AGAAGAGCATTCTATTTCCA+TGG	0.535708	2.2:-64640839	None:intergenic
TGATGAGGAGGAAATAAGAA+TGG	0.564678	2.2:+64640879	MS.gene96184:CDS
TTTACTTGTTGATGATGAGG+AGG	0.584354	2.2:+64640867	MS.gene96184:CDS
GTGGTTATCAGATGCAACCA+TGG	0.627194	2.2:+64640822	MS.gene96184:CDS
ACCTGTGAGGGTGACAATGA+TGG	0.639274	2.2:+64640727	MS.gene96184:CDS
GAGGACAGTGCAGATTCTGA+AGG	0.642136	2.2:+64640628	MS.gene96184:CDS
GCCCAAAGTAATTAGCAGCA+GGG	0.657577	2.2:-64640678	None:intergenic
GCTGAGTCATGTACCTGTGA+GGG	0.671916	2.2:+64640715	MS.gene96184:CDS
TGCTGAGTCATGTACCTGTG+AGG	0.698564	2.2:+64640714	MS.gene96184:CDS
GGTTGCATCTGATAACCACA+TGG	0.727761	2.2:-64640818	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGAATGGTTAATGTTAATT+TGG	+	chr2.2:64640894-64640913	MS.gene96184:CDS	20.0%
!!!	ATGTTGATGATAAACTATTT+TGG	+	chr2.2:64640920-64640939	MS.gene96184:CDS	20.0%
!!!	TGTTGATGATAAACTATTTT+GGG	+	chr2.2:64640921-64640940	MS.gene96184:CDS	20.0%
	AAGGAAGATGAGAATGATAA+AGG	+	chr2.2:64640766-64640785	MS.gene96184:CDS	30.0%
	AGGAAGATGAGAATGATAAA+GGG	+	chr2.2:64640767-64640786	MS.gene96184:CDS	30.0%
	ATAGAGTAAAGAAGCCAATA+AGG	-	chr2.2:64640657-64640676	None:intergenic	30.0%
!	TTCATCTTTGTTGCTTATTG+AGG	+	chr2.2:64640609-64640628	MS.gene96184:CDS	30.0%
!!	ACTATTTTGGGAGATATGTA+TGG	+	chr2.2:64640933-64640952	MS.gene96184:CDS	30.0%
	TCCTCATCATCAACAAGTAA+AGG	-	chr2.2:64640868-64640887	None:intergenic	35.0%
	TGATGAGGAGGAAATAAGAA+TGG	+	chr2.2:64640879-64640898	MS.gene96184:CDS	35.0%
	TGGTATGTGTAGCTTAGTTA+AGG	+	chr2.2:64640747-64640766	MS.gene96184:CDS	35.0%
!!	ACCTTTACTTGTTGATGATG+AGG	+	chr2.2:64640864-64640883	MS.gene96184:CDS	35.0%
!!	AGAAGAGCATTCTATTTCCA+TGG	-	chr2.2:64640842-64640861	None:intergenic	35.0%
!!	TTTACTTGTTGATGATGAGG+AGG	+	chr2.2:64640867-64640886	MS.gene96184:CDS	35.0%
	ACCCTGCTGCTAATTACTTT+GGG	+	chr2.2:64640677-64640696	MS.gene96184:CDS	40.0%
	GATGAGAATGATAAAGGGTC+AGG	+	chr2.2:64640772-64640791	MS.gene96184:CDS	40.0%
	GGGAGATATGTATGGAAGTA+GGG	+	chr2.2:64640941-64640960	MS.gene96184:CDS	40.0%
	TACCCTGCTGCTAATTACTT+TGG	+	chr2.2:64640676-64640695	MS.gene96184:CDS	40.0%
	TGCTAATTACTTTGGGCACA+AGG	+	chr2.2:64640684-64640703	MS.gene96184:CDS	40.0%
	TGGGAGATATGTATGGAAGT+AGG	+	chr2.2:64640940-64640959	MS.gene96184:CDS	40.0%
!	GATTCTGAAGGTGACCTTAT+TGG	+	chr2.2:64640640-64640659	MS.gene96184:CDS	40.0%
	GCCCAAAGTAATTAGCAGCA+GGG	-	chr2.2:64640681-64640700	None:intergenic	45.0%
	GGTTGCATCTGATAACCACA+TGG	-	chr2.2:64640821-64640840	None:intergenic	45.0%
	GTGGTTATCAGATGCAACCA+TGG	+	chr2.2:64640822-64640841	MS.gene96184:CDS	45.0%
	TGCCCAAAGTAATTAGCAGC+AGG	-	chr2.2:64640682-64640701	None:intergenic	45.0%
!!!	GCTGTTTTAGCTCTACCATG+TGG	+	chr2.2:64640803-64640822	MS.gene96184:CDS	45.0%
	ACCATCATTGTCACCCTCAC+AGG	-	chr2.2:64640731-64640750	None:intergenic	50.0%
	ACCTGTGAGGGTGACAATGA+TGG	+	chr2.2:64640727-64640746	MS.gene96184:CDS	50.0%
	GAGGACAGTGCAGATTCTGA+AGG	+	chr2.2:64640628-64640647	MS.gene96184:CDS	50.0%
	GCTGAGTCATGTACCTGTGA+GGG	+	chr2.2:64640715-64640734	MS.gene96184:CDS	50.0%
	TGCTGAGTCATGTACCTGTG+AGG	+	chr2.2:64640714-64640733	MS.gene96184:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	64640586	64640972	64640586	ID=MS.gene96184
chr2.2	mRNA	64640586	64640972	64640586	ID=MS.gene96184.t1;Parent=MS.gene96184
chr2.2	exon	64640586	64640972	64640586	ID=MS.gene96184.t1.exon1;Parent=MS.gene96184.t1
chr2.2	CDS	64640586	64640972	64640586	ID=cds.MS.gene96184.t1;Parent=MS.gene96184.t1

Gene Sequence

>MS.gene96184

ATGAATGATATGGATGTATCATGTTCATCTTTGTTGCTTATTGAGGACAGTGCAGATTCTGAAGGTGACCTTATTGGCTTCTTTACTCTATACCCTGCTGCTAATTACTTTGGGCACAAGGAAGATGATGCTGAGTCATGTACCTGTGAGGGTGACAATGATGGTATGTGTAGCTTAGTTAAGGAAGATGAGAATGATAAAGGGTCAGGTGAATTTTGCTGTTTTAGCTCTACCATGTGGTTATCAGATGCAACCATGGAAATAGAATGCTCTTCTTCACCTTTACTTGTTGATGATGAGGAGGAAATAAGAATGGTTAATGTTAATTTGGATGATGTTGATGATAAACTATTTTGGGAGATATGTATGGAAGTAGGGTACCCTTGA

Protein sequence

>MS.gene96184.t1

MNDMDVSCSSLLLIEDSADSEGDLIGFFTLYPAANYFGHKEDDAESCTCEGDNDGMCSLVKEDENDKGSGEFCCFSSTMWLSDATMEIECSSSPLLVDDEEEIRMVNVNLDDVDDKLFWEICMEVGYP