AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene96260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96260.t1 MTR_2g018960 98.441 898 10 1 1 898 1 894 0.0 1831
MS.gene96260.t1 MTR_8g028075 23.377 308 212 8 525 828 255 542 2.87e-12 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96260.t1 AT5G54830 64.151 901 306 9 7 896 12 906 0.0 1199

Find 243 sgRNAs with CRISPR-Local

Find 274 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACGTTTAAAAGGATAATT+TGG 0.111755 2.2:-65608581 MS.gene96260:CDS
ATTCCAACTGCATCAAATTT+TGG 0.141719 2.2:-65610541 MS.gene96260:CDS
AGCTCAACACATGTTAAATT+TGG 0.155830 2.2:-65608684 MS.gene96260:CDS
CGGCTAACATGACTGTTATT+TGG 0.161465 2.2:-65609979 MS.gene96260:CDS
TGCTCCCCATTTGATGGTTT+TGG 0.164073 2.2:+65608606 None:intergenic
GCATCTATCTACCTGTTTAA+AGG 0.181231 2.2:+65608228 None:intergenic
GGATATTTGGAAGAGGAAAT+TGG 0.191249 2.2:-65608359 MS.gene96260:CDS
TCACTTGGGCAATGATAATT+TGG 0.204909 2.2:-65608446 MS.gene96260:CDS
TTGAACAACGAATGTTAAAT+TGG 0.224862 2.2:+65610615 None:intergenic
GCAGGATTTGCTTCCAGTTT+TGG 0.242070 2.2:-65608898 MS.gene96260:CDS
ATCAACAGGTTACCTTCTTT+TGG 0.247908 2.2:-65609367 MS.gene96260:CDS
CGAGCACGGGATCGGATATT+TGG 0.251463 2.2:-65608372 MS.gene96260:CDS
AACATTTGAAGGGTGATAAT+TGG 0.254622 2.2:-65608800 MS.gene96260:CDS
TCTCAATTTGATATGTTATC+TGG 0.254696 2.2:-65610736 MS.gene96260:CDS
TTCGGTGTATGAGAAGAAAA+TGG 0.264953 2.2:-65609456 MS.gene96260:CDS
TGATCCCTGTTCTTTCTGTT+TGG 0.265660 2.2:-65610567 MS.gene96260:CDS
TATCACCCTTCAAATGTTTC+AGG 0.272420 2.2:+65608805 None:intergenic
GGTCTTCCGTTTGAGACTTT+TGG 0.272852 2.2:-65609893 MS.gene96260:CDS
GATCCCTGTTCTTTCTGTTT+GGG 0.275825 2.2:-65610566 MS.gene96260:CDS
TTGGCAGGATTTGGCGGGTT+AGG 0.288091 2.2:+65609731 None:intergenic
TAAAGAATCTTGGCAGGATT+TGG 0.299021 2.2:+65609722 None:intergenic
CCTTGGTTCCATCTTGTAGT+AGG 0.312930 2.2:-65609091 MS.gene96260:CDS
GCATGGGGTATCTTGCTTCC+TGG 0.316197 2.2:-65608849 MS.gene96260:CDS
CTTGCTTCCTGGAGGTATCT+TGG 0.318098 2.2:-65608838 MS.gene96260:CDS
CTAGTCCCACGGAACTAGTT+TGG 0.323814 2.2:-65609517 MS.gene96260:CDS
GTTATGAATTACATGGCTTT+TGG 0.328103 2.2:-65610316 MS.gene96260:CDS
TGGTGCTGATGTGGCGGTTA+CGG 0.329797 2.2:-65610254 MS.gene96260:CDS
GGTTATCTTGCCATAGGATT+TGG 0.331663 2.2:-65609293 MS.gene96260:CDS
ACCTGTTTAAAGGTTCTAAC+TGG 0.335200 2.2:+65608238 None:intergenic
TCAATTGAGATTGGTTTAGA+AGG 0.336814 2.2:-65610349 MS.gene96260:CDS
GTTAACACTTACTGGATTGA+TGG 0.338446 2.2:-65609209 MS.gene96260:CDS
AAACCATGCACGGCCAAAAC+TGG 0.343302 2.2:+65608885 None:intergenic
GAAGGAGAAGGAGAAGAATA+AGG 0.344994 2.2:-65610464 MS.gene96260:CDS
GATTCTTTACATCAACAAAA+AGG 0.346577 2.2:-65609708 MS.gene96260:CDS
ATGATAATTTGGTTCTTAGT+AGG 0.347365 2.2:-65608435 MS.gene96260:CDS
GGTGCTGATGTGGCGGTTAC+GGG 0.348511 2.2:-65610253 MS.gene96260:CDS
GGGAGCAGCCAACGTTTAAA+AGG 0.348957 2.2:-65608590 MS.gene96260:CDS
TCCTGAGGCTCATGGTGTTC+AGG 0.352665 2.2:-65609540 MS.gene96260:CDS
GGCTAACATGACTGTTATTT+GGG 0.359054 2.2:-65609978 MS.gene96260:CDS
TCAATGATGTTGTTACATTC+TGG 0.359602 2.2:+65609059 None:intergenic
GGAGAGTGGAGGTTGTGGAC+GGG 0.363911 2.2:-65609430 MS.gene96260:CDS
AATGATACAAGGTTGATATA+CGG 0.367078 2.2:-65610088 MS.gene96260:CDS
TGGCAGGATTTGGCGGGTTA+GGG 0.367973 2.2:+65609732 None:intergenic
TTGGTCGTGTTAACACTTAC+TGG 0.371151 2.2:-65609217 MS.gene96260:CDS
TTTGTTGATGTAAAGAATCT+TGG 0.376375 2.2:+65609712 None:intergenic
ATTGGTTTAGAAGGTGCAAC+TGG 0.377218 2.2:-65610340 MS.gene96260:CDS
ATGACTGTTATTTGGGCGTT+GGG 0.381712 2.2:-65609971 MS.gene96260:CDS
AGCTCGGAATCAGTAGCATT+AGG 0.382038 2.2:+65610283 None:intergenic
CAACTCCACTTAAAGTCATA+TGG 0.387100 2.2:-65609031 MS.gene96260:CDS
TATGCTTTGGAAAACGTTCA+TGG 0.388460 2.2:-65608471 MS.gene96260:CDS
TGATCAGGAGTGTTCCTATC+AGG 0.389061 2.2:+65609491 None:intergenic
TTTGGGAGACAGATATGCTT+TGG 0.389075 2.2:-65608484 MS.gene96260:CDS
GTATAACAACAGTGTTGTTC+TGG 0.392054 2.2:-65609393 MS.gene96260:CDS
TTTACTGGCATGAAGCATTT+GGG 0.393832 2.2:-65608501 MS.gene96260:CDS
GTTGGCTGCTCCCCATTTGA+TGG 0.397291 2.2:+65608600 None:intergenic
TGTTGTTCTGGATGATCAAC+AGG 0.405150 2.2:-65609381 MS.gene96260:CDS
TAGTCCCACGGAACTAGTTT+GGG 0.406291 2.2:-65609516 MS.gene96260:CDS
AACGTTTAAAAGGATAATTT+GGG 0.407306 2.2:-65608580 MS.gene96260:CDS
GGAAGACGGGTTGCCATTTG+TGG 0.409956 2.2:-65610224 MS.gene96260:CDS
TTTCGAATCCGATTTCACTA+TGG 0.411362 2.2:-65610815 MS.gene96260:CDS
AAGGAGGATTCAATTGAGAT+TGG 0.412374 2.2:-65610358 MS.gene96260:CDS
AACTATGTTTGATAATTGTA+AGG 0.416063 2.2:-65610422 MS.gene96260:CDS
GGAAGAGGAAATTGGGTGCT+TGG 0.421239 2.2:-65608351 MS.gene96260:CDS
GTTCCATTGGTTGTTTCCAC+TGG 0.421377 2.2:-65609770 MS.gene96260:CDS
AATTGATGAAGAATGTTACT+TGG 0.421489 2.2:-65610594 MS.gene96260:CDS
CTTGGTTCCATCTTGTAGTA+GGG 0.421955 2.2:-65609090 MS.gene96260:CDS
ATTGGTTGTTTCCACTGGTC+CGG 0.424350 2.2:-65609765 MS.gene96260:CDS
TTTGTTGAAAGTGGAATTGT+TGG 0.425789 2.2:+65608294 None:intergenic
CACTTACTGGATTGATGGAC+AGG 0.426039 2.2:-65609204 MS.gene96260:CDS
TCCAGTTAGAACCTTTAAAC+AGG 0.426564 2.2:-65608239 MS.gene96260:CDS
ATTGCCCATATGACTTTAAG+TGG 0.427061 2.2:+65609026 None:intergenic
GAACTGTTTCGAGTGCATTT+CGG 0.427588 2.2:+65610847 None:intergenic
AACTAGTTTGGGCTCCTGAT+AGG 0.428703 2.2:-65609505 MS.gene96260:CDS
AGGGTCCCAAAACCATCAAA+TGG 0.429791 2.2:-65608612 MS.gene96260:CDS
TGGAGAGTGGAGGTTGTGGA+CGG 0.432590 2.2:-65609431 MS.gene96260:CDS
ATGTTGAACCATAGTGAAAT+CGG 0.434450 2.2:+65610807 None:intergenic
TTTCGAGTGCATTTCGGAGC+AGG 0.434921 2.2:+65610853 None:intergenic
TTCACAGGCTTCTGCAAGAA+TGG 0.438288 2.2:-65608265 MS.gene96260:CDS
GAGAGTGGAGGTTGTGGACG+GGG 0.443990 2.2:-65609429 MS.gene96260:CDS
GAATATTTGCACATAATTGT+TGG 0.444607 2.2:-65608558 MS.gene96260:CDS
CATGTTTACTTGCAATACTC+AGG 0.444996 2.2:-65608768 MS.gene96260:CDS
ATATCCCAAACAGAAAGAAC+AGG 0.448559 2.2:+65610562 None:intergenic
GGACCTTCATATATCGAATC+AGG 0.448577 2.2:+65610130 None:intergenic
ATCTCCTCCCTCGTTCTTCG+TGG 0.449854 2.2:+65610495 None:intergenic
TTCAACAAATAAAGATTCAC+AGG 0.453086 2.2:-65608280 MS.gene96260:CDS
TGATCAATCTGTTGTTCAAT+CGG 0.455252 2.2:-65609999 MS.gene96260:CDS
CTTCAAATGTTTCAGGTACC+TGG 0.455447 2.2:+65608812 None:intergenic
CTGGAGGTATCTTGGCAGCC+AGG 0.457385 2.2:-65608830 MS.gene96260:CDS
TGGCTCTGATGTGCATTGGT+GGG 0.457456 2.2:-65610716 MS.gene96260:CDS
AGACCTTTGAGTCAGGTTGA+TGG 0.457589 2.2:-65610028 MS.gene96260:CDS
GGGTCCCAAAACCATCAAAT+GGG 0.459394 2.2:-65608611 MS.gene96260:CDS
GGGAGGAGATGGGAAGGAGA+AGG 0.463195 2.2:-65610482 MS.gene96260:CDS
ACTGATTCCGAGCTTATGAT+TGG 0.463497 2.2:-65610274 MS.gene96260:CDS
GCTCGGAATCAGTAGCATTA+GGG 0.465085 2.2:+65610284 None:intergenic
CTGGCTCTGATGTGCATTGG+TGG 0.465288 2.2:-65610717 MS.gene96260:CDS
GGGTTGGAGAGTGGAGGTTG+TGG 0.465828 2.2:-65609435 MS.gene96260:CDS
TTCACCAAACCAACTCTATC+CGG 0.466393 2.2:+65610109 None:intergenic
TGACCAAAATTTGATGCAGT+TGG 0.469612 2.2:+65610538 None:intergenic
AACCAGCTAATGCATTCCTC+AGG 0.470039 2.2:-65608632 MS.gene96260:CDS
GATATTTGGAAGAGGAAATT+GGG 0.472653 2.2:-65608358 MS.gene96260:CDS
CTTAAAGTCATATGGGCAAT+GGG 0.473317 2.2:-65609023 MS.gene96260:CDS
CATCTTGTAGTAGGGGCAAA+AGG 0.473661 2.2:-65609082 MS.gene96260:CDS
ATATAAGAGACCTTTGAGTC+AGG 0.477326 2.2:-65610035 MS.gene96260:CDS
AAAACGTTCATGGACTCACT+TGG 0.477332 2.2:-65608461 MS.gene96260:CDS
ACGAAGAACGAGGGAGGAGA+TGG 0.478754 2.2:-65610493 MS.gene96260:CDS
TAATGCGGTAAAACAGTATC+CGG 0.478815 2.2:+65609935 None:intergenic
GAATGATAGTAGATCTGATC+AGG 0.479177 2.2:+65609476 None:intergenic
CATGACTGTTATTTGGGCGT+TGG 0.479843 2.2:-65609972 MS.gene96260:CDS
GATAAACAGTTATACATATG+TGG 0.480665 2.2:-65609261 MS.gene96260:CDS
TTACAATTATCAAACATAGT+TGG 0.481504 2.2:+65610424 None:intergenic
GAATCTTTATTTGTTGAAAG+TGG 0.482826 2.2:+65608285 None:intergenic
TATGGGCAATGGGTAGTAGA+TGG 0.485381 2.2:-65609013 MS.gene96260:CDS
ATCGGATTCGAAATCGATGA+AGG 0.494635 2.2:+65610825 None:intergenic
ATGACTTTAAGTGGAGTTGT+GGG 0.494726 2.2:+65609035 None:intergenic
TTTGCGGTTGCTGAGCTTCG+CGG 0.494762 2.2:-65608717 MS.gene96260:CDS
ATTGGAATTTACACGTCCCT+TGG 0.496061 2.2:-65609108 MS.gene96260:CDS
GCCTGAACACCATGAGCCTC+AGG 0.496343 2.2:+65609539 None:intergenic
CAGTGTGGCATTCCCACCAA+TGG 0.497818 2.2:+65609595 None:intergenic
CATTCCACTGCCAAATCCTA+TGG 0.498718 2.2:+65609283 None:intergenic
CCGCGAGAAGCAGCGAGCAC+GGG 0.500795 2.2:-65608385 MS.gene96260:CDS
TCGGTGTATGAGAAGAAAAT+GGG 0.502362 2.2:-65609455 MS.gene96260:CDS
TGATCAGATCTACTATCATT+CGG 0.502694 2.2:-65609474 MS.gene96260:CDS
GGCTCTGATGTGCATTGGTG+GGG 0.503046 2.2:-65610715 MS.gene96260:CDS
TGATTCGATATATGAAGGTC+CGG 0.503314 2.2:-65610128 MS.gene96260:CDS
ACCGCGAGAAGCAGCGAGCA+CGG 0.506718 2.2:-65608386 MS.gene96260:CDS
GAAGAAAATGGGTTGGAGAG+TGG 0.506742 2.2:-65609444 MS.gene96260:CDS
ACGTTGGAGTTTGAATTTGA+AGG 0.509519 2.2:-65610377 MS.gene96260:CDS
TGTCCTGATTCGATATATGA+AGG 0.511057 2.2:-65610133 MS.gene96260:CDS
ACTTAAAGTCATATGGGCAA+TGG 0.511086 2.2:-65609024 MS.gene96260:CDS
TGGTTCTGCAGAAGCAGAGC+AGG 0.512384 2.2:-65608916 MS.gene96260:CDS
GCTGGTGGTCCTGAGGCTCA+TGG 0.515891 2.2:-65609548 MS.gene96260:CDS
TGCTGATGCAAAAGTTCCAT+TGG 0.516316 2.2:-65609783 MS.gene96260:CDS
AGAATCTTGGCAGGATTTGG+CGG 0.516390 2.2:+65609725 None:intergenic
TTTGATAATTTCACCAATGG+AGG 0.517652 2.2:-65610676 MS.gene96260:CDS
GAATCTTGGCAGGATTTGGC+GGG 0.518974 2.2:+65609726 None:intergenic
AGATGGGAAGGAGAAGGAGA+AGG 0.521339 2.2:-65610476 MS.gene96260:CDS
AGAAGCAGCGAGCACGGGAT+CGG 0.521776 2.2:-65608380 MS.gene96260:CDS
TCTTTATCACCAGCATCTAA+AGG 0.522322 2.2:+65609824 None:intergenic
AGGTCCGGATAGAGTTGGTT+TGG 0.522338 2.2:-65610113 MS.gene96260:CDS
TTGATGTAAAGAATCTTGGC+AGG 0.522540 2.2:+65609716 None:intergenic
AACTCCACTTAAAGTCATAT+GGG 0.525949 2.2:-65609030 MS.gene96260:CDS
GTGAGATGCAAAACAGAAAA+CGG 0.526080 2.2:-65609140 MS.gene96260:CDS
CGAAGAACGAGGGAGGAGAT+GGG 0.526235 2.2:-65610492 MS.gene96260:CDS
ATTGATGAAGAATGTTACTT+GGG 0.527040 2.2:-65610593 MS.gene96260:CDS
TATGACTTTAAGTGGAGTTG+TGG 0.527868 2.2:+65609034 None:intergenic
CGCATCAGCTGAACAAGTAT+AGG 0.528232 2.2:+65608939 None:intergenic
TAGGAGTGATGGGGTTTCGT+TGG 0.530705 2.2:-65610062 MS.gene96260:CDS
CAGTTATACATATGTGGGAT+GGG 0.531034 2.2:-65609255 MS.gene96260:CDS
GAACGAGGGAGGAGATGGGA+AGG 0.531311 2.2:-65610488 MS.gene96260:CDS
TATATTACTACAGATCCCAT+TGG 0.531920 2.2:-65609611 MS.gene96260:CDS
TTTAGATGCTGGTGATAAAG+AGG 0.532835 2.2:-65609822 MS.gene96260:CDS
CTTTGACAATGTCCAAAAGA+AGG 0.532849 2.2:+65609355 None:intergenic
TGTTGGACTCAAGAGATCAA+TGG 0.533020 2.2:+65608311 None:intergenic
CAGGTACCTGAAACATTTGA+AGG 0.535559 2.2:-65608811 MS.gene96260:CDS
TGAAACAATGAATCCTCCAT+TGG 0.538253 2.2:+65610663 None:intergenic
AAGAGTGGTTATCTTGCCAT+AGG 0.539558 2.2:-65609299 MS.gene96260:CDS
ATGATACAAGGTTGATATAC+GGG 0.540824 2.2:-65610087 MS.gene96260:CDS
AGAAAACGGCATGATTACAT+TGG 0.544819 2.2:-65609126 MS.gene96260:CDS
GCTTGTATAGAAAACGTAAC+CGG 0.545686 2.2:+65609653 None:intergenic
GGGTTAGGGTAATGCAAAGC+CGG 0.546867 2.2:+65609746 None:intergenic
AAATGACCAAAAGTCTCAAA+CGG 0.548603 2.2:+65609887 None:intergenic
TGGGTTGATGACAATGGTGT+TGG 0.549825 2.2:-65609236 MS.gene96260:CDS
CAGAATGTGAATGATTGTAA+AGG 0.550009 2.2:-65609848 MS.gene96260:CDS
ACAGTTATACATATGTGGGA+TGG 0.551164 2.2:-65609256 MS.gene96260:CDS
CAAGGTTGATATACGGGCAT+AGG 0.552235 2.2:-65610081 MS.gene96260:CDS
AGTGTGGCATTCCCACCAAT+GGG 0.555344 2.2:+65609596 None:intergenic
TTACCATCAACCTGACTCAA+AGG 0.556389 2.2:+65610025 None:intergenic
CTGGCTGCCAAGATACCTCC+AGG 0.556842 2.2:+65608831 None:intergenic
ATGTTTACTTGCAATACTCA+GGG 0.560570 2.2:-65608767 MS.gene96260:CDS
ATATACGGGCATAGGAGTGA+TGG 0.560999 2.2:-65610073 MS.gene96260:CDS
ATCCGAAAACGAACAAGGAT+AGG 0.561641 2.2:+65610884 None:intergenic
TATACGGGCATAGGAGTGAT+GGG 0.562005 2.2:-65610072 MS.gene96260:CDS
TTGGTTCCATCTTGTAGTAG+GGG 0.563124 2.2:-65609089 MS.gene96260:CDS
CTTGCCATAGGATTTGGCAG+TGG 0.563630 2.2:-65609287 MS.gene96260:CDS
GAGAGTGGAAAGAGGAGTGC+CGG 0.564285 2.2:-65609672 MS.gene96260:CDS
TTGGAGTTTGAATTTGAAGG+AGG 0.564306 2.2:-65610374 MS.gene96260:CDS
ATCAGCACCAATCATAAGCT+CGG 0.565906 2.2:+65610267 None:intergenic
ATAAACAGTTATACATATGT+GGG 0.568097 2.2:-65609260 MS.gene96260:CDS
GTGAAGATGGATCTGTCGAA+GGG 0.568991 2.2:-65610159 MS.gene96260:CDS
AACACTGTTGTTATACATGT+CGG 0.570128 2.2:+65609400 None:intergenic
TGTGTGAAGAATAGTGAAGA+TGG 0.570545 2.2:-65610172 MS.gene96260:CDS
ATAAAGCGCAACATCATGCC+CGG 0.572365 2.2:+65609631 None:intergenic
CTAACTGAGACAATTTATGC+TGG 0.573223 2.2:-65609566 MS.gene96260:CDS
GGAAGCTCCTGTGTTGAGAG+TGG 0.577718 2.2:-65609687 MS.gene96260:CDS
CGGGATCGGATATTTGGAAG+AGG 0.577832 2.2:-65608366 MS.gene96260:CDS
CCTGTGTTGAGAGTGGAAAG+AGG 0.578222 2.2:-65609680 MS.gene96260:CDS
GAAAATGGGTTGGAGAGTGG+AGG 0.579894 2.2:-65609441 MS.gene96260:CDS
TGTAAAGGTCCTTTAGATGC+TGG 0.582856 2.2:-65609833 MS.gene96260:CDS
ACCCTGAGGAATGCATTAGC+TGG 0.583353 2.2:+65608630 None:intergenic
AACTGGGGTTATGAATTACA+TGG 0.584156 2.2:-65610323 MS.gene96260:CDS
TATCTGGCTCTGATGTGCAT+TGG 0.584450 2.2:-65610720 MS.gene96260:CDS
ATTACTACAGATCCCATTGG+TGG 0.584690 2.2:-65609608 MS.gene96260:CDS
TTGGTTTAGAAGGTGCAACT+GGG 0.587129 2.2:-65610339 MS.gene96260:CDS
GTAATGAACATCTTACTGAA+AGG 0.589810 2.2:-65608989 MS.gene96260:CDS
CCCGTGCTCGCTGCTTCTCG+CGG 0.593232 2.2:+65608385 None:intergenic
GTTGGGGAAGATGAGAGCTC+CGG 0.594601 2.2:-65609954 MS.gene96260:CDS
TTTATGATGTTTCTTGCATG+GGG 0.595008 2.2:-65608864 MS.gene96260:CDS
ATACTTGTTCAGCTGATGCG+TGG 0.598725 2.2:-65608936 MS.gene96260:CDS
AGTGAAGATGGATCTGTCGA+AGG 0.598930 2.2:-65610160 MS.gene96260:CDS
TTGGTTTGGTGAATGATACA+AGG 0.599261 2.2:-65610099 MS.gene96260:CDS
TATGAAGGTCCGGATAGAGT+TGG 0.600834 2.2:-65610118 MS.gene96260:CDS
AGGAGCCCAAACTAGTTCCG+TGG 0.607300 2.2:+65609511 None:intergenic
GGAAGACCGCCATGGTTCTG+AGG 0.608095 2.2:+65609908 None:intergenic
ACCAGCTAATGCATTCCTCA+GGG 0.608346 2.2:-65608631 MS.gene96260:CDS
TGCATATCCGAAAACGAACA+AGG 0.609136 2.2:+65610879 None:intergenic
GCTTATGATTGGTGCTGATG+TGG 0.613221 2.2:-65610263 MS.gene96260:CDS
AAATTGGGTGCTTGGCAATG+AGG 0.614704 2.2:-65608343 MS.gene96260:CDS
AGAGTGGAGGTTGTGGACGG+GGG 0.616025 2.2:-65609428 MS.gene96260:CDS
AATTTATGCTGGTGGTCCTG+AGG 0.616178 2.2:-65609555 MS.gene96260:CDS
GGAGCCCAAACTAGTTCCGT+GGG 0.616346 2.2:+65609512 None:intergenic
AAACATCATAAAACCATGCA+CGG 0.617454 2.2:+65608875 None:intergenic
CTCAGTTAGATTCCTCAGTG+TGG 0.617997 2.2:+65609580 None:intergenic
TGTATGAGAAGAAAATGGGT+TGG 0.619184 2.2:-65609451 MS.gene96260:CDS
ACTGAGACAATTTATGCTGG+TGG 0.620789 2.2:-65609563 MS.gene96260:CDS
TTACTACAGATCCCATTGGT+GGG 0.621508 2.2:-65609607 MS.gene96260:CDS
TGGTGTTCAGGCTAGTCCCA+CGG 0.624788 2.2:-65609528 MS.gene96260:CDS
GGACCAGTGGAAACAACCAA+TGG 0.625228 2.2:+65609767 None:intergenic
AAACGTTCATGGACTCACTT+GGG 0.625372 2.2:-65608460 MS.gene96260:CDS
TGGGGTATCTTGCTTCCTGG+AGG 0.628780 2.2:-65608846 MS.gene96260:CDS
GTAATGCAAAGCCGGACCAG+TGG 0.629267 2.2:+65609754 None:intergenic
CCTCTTTCCACTCTCAACAC+AGG 0.640164 2.2:+65609680 None:intergenic
AGGTACCTGAAACATTTGAA+GGG 0.647814 2.2:-65608810 MS.gene96260:CDS
TATGATTGGTGCTGATGTGG+CGG 0.648845 2.2:-65610260 MS.gene96260:CDS
TGGTTTAGAAGGTGCAACTG+GGG 0.650155 2.2:-65610338 MS.gene96260:CDS
TGACTGTTATTTGGGCGTTG+GGG 0.650959 2.2:-65609970 MS.gene96260:CDS
CATTATCTTCCTCAGAACCA+TGG 0.653127 2.2:-65609917 MS.gene96260:CDS
TCAATATCTATTGCTGCTCG+TGG 0.653461 2.2:-65609329 MS.gene96260:CDS
GTGGGATGGGTTGATGACAA+TGG 0.653502 2.2:-65609242 MS.gene96260:CDS
TCTCAAACGGAAGACCGCCA+TGG 0.654243 2.2:+65609900 None:intergenic
CTACTACAAGATGGAACCAA+GGG 0.657528 2.2:+65609092 None:intergenic
AATATCACCACGAAGAACGA+GGG 0.661469 2.2:-65610502 MS.gene96260:CDS
AAATATCACCACGAAGAACG+AGG 0.663007 2.2:-65610503 MS.gene96260:CDS
ATCACCACGAAGAACGAGGG+AGG 0.667011 2.2:-65610499 MS.gene96260:CDS
CCTACTACAAGATGGAACCA+AGG 0.677148 2.2:+65609091 None:intergenic
GGTCCCAAAACCATCAAATG+GGG 0.677788 2.2:-65608610 MS.gene96260:CDS
TTCGAGCATACATCTTACAC+AGG 0.677952 2.2:-65609177 MS.gene96260:CDS
TATCCCAAACAGAAAGAACA+GGG 0.684383 2.2:+65610563 None:intergenic
TGGTTCTGAGGAAGATAATG+CGG 0.694284 2.2:+65609920 None:intergenic
ATACGGGCATAGGAGTGATG+GGG 0.707780 2.2:-65610071 MS.gene96260:CDS
TGAAGATGGATCTGTCGAAG+GGG 0.709595 2.2:-65610158 MS.gene96260:CDS
ATGGTTCAACATCAACTCAG+AGG 0.724145 2.2:-65610796 MS.gene96260:CDS
TATCTTCCTCAGAACCATGG+CGG 0.760198 2.2:-65609914 MS.gene96260:CDS
TTGGTGGGAATGCCACACTG+AGG 0.762184 2.2:-65609592 MS.gene96260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACGTTTAAAAGGATAATTT+GGG - chr2.2:65610566-65610585 MS.gene96260:CDS 20.0%
!! ATAAAAAAATCATCCACAAA+TGG + chr2.2:65608938-65608957 None:intergenic 20.0%
!! ATAAACAGTTATACATATGT+GGG - chr2.2:65609886-65609905 MS.gene96260:CDS 20.0%
!! TTACAATTATCAAACATAGT+TGG + chr2.2:65608725-65608744 None:intergenic 20.0%
!!! AACTATGTTTGATAATTGTA+AGG - chr2.2:65608724-65608743 MS.gene96260:CDS 20.0%
!!! AATCATCAATGATTTTGAAA+TGG + chr2.2:65608376-65608395 None:intergenic 20.0%
! AATTGATGAAGAATGTTACT+TGG - chr2.2:65608552-65608571 MS.gene96260:CDS 25.0%
! CAACGTTTAAAAGGATAATT+TGG - chr2.2:65610565-65610584 MS.gene96260:CDS 25.0%
! GAATATTTGCACATAATTGT+TGG - chr2.2:65610588-65610607 MS.gene96260:CDS 25.0%
! GATAAACAGTTATACATATG+TGG - chr2.2:65609885-65609904 MS.gene96260:CDS 25.0%
! GATTCTTTACATCAACAAAA+AGG - chr2.2:65609438-65609457 MS.gene96260:CDS 25.0%
! TCTCAATTTGATATGTTATC+TGG - chr2.2:65608410-65608429 MS.gene96260:CDS 25.0%
! TTCAACAAATAAAGATTCAC+AGG - chr2.2:65610866-65610885 MS.gene96260:CDS 25.0%
! TTGAACAACGAATGTTAAAT+TGG + chr2.2:65608534-65608553 None:intergenic 25.0%
! TTTGTTGATGTAAAGAATCT+TGG + chr2.2:65609437-65609456 None:intergenic 25.0%
!! ATTGATGAAGAATGTTACTT+GGG - chr2.2:65608553-65608572 MS.gene96260:CDS 25.0%
!! CAAATTATCCTTTTAAACGT+TGG + chr2.2:65610567-65610586 None:intergenic 25.0%
!! GAATCTTTATTTGTTGAAAG+TGG + chr2.2:65610864-65610883 None:intergenic 25.0%
!! TTTTATGATGTTTCTTGCAT+GGG - chr2.2:65610281-65610300 MS.gene96260:CDS 25.0%
!!! AATGATACAAGGTTGATATA+CGG - chr2.2:65609058-65609077 MS.gene96260:CDS 25.0%
!!! ATGATAATTTGGTTCTTAGT+AGG - chr2.2:65610711-65610730 MS.gene96260:CDS 25.0%
!!! GATTTTGATAATTTCACCAA+TGG - chr2.2:65608467-65608486 MS.gene96260:CDS 25.0%
!!! TAATTGTAAGGTTTTGTCTA+AGG - chr2.2:65608736-65608755 MS.gene96260:CDS 25.0%
AAACATCATAAAACCATGCA+CGG + chr2.2:65610274-65610293 None:intergenic 30.0%
AAATGACCAAAAGTCTCAAA+CGG + chr2.2:65609262-65609281 None:intergenic 30.0%
AACACTGTTGTTATACATGT+CGG + chr2.2:65609749-65609768 None:intergenic 30.0%
AACATTTGAAGGGTGATAAT+TGG - chr2.2:65610346-65610365 MS.gene96260:CDS 30.0%
AACTCCACTTAAAGTCATAT+GGG - chr2.2:65610116-65610135 MS.gene96260:CDS 30.0%
AGCTCAACACATGTTAAATT+TGG - chr2.2:65610462-65610481 MS.gene96260:CDS 30.0%
ATGTTGAACCATAGTGAAAT+CGG + chr2.2:65608342-65608361 None:intergenic 30.0%
ATGTTTACTTGCAATACTCA+GGG - chr2.2:65610379-65610398 MS.gene96260:CDS 30.0%
CAGAATGTGAATGATTGTAA+AGG - chr2.2:65609298-65609317 MS.gene96260:CDS 30.0%
GATATTTGGAAGAGGAAATT+GGG - chr2.2:65610788-65610807 MS.gene96260:CDS 30.0%
GTAATGAACATCTTACTGAA+AGG - chr2.2:65610157-65610176 MS.gene96260:CDS 30.0%
TATATTACTACAGATCCCAT+TGG - chr2.2:65609535-65609554 MS.gene96260:CDS 30.0%
TCAATGATGTTGTTACATTC+TGG + chr2.2:65610090-65610109 None:intergenic 30.0%
TGATCAATCTGTTGTTCAAT+CGG - chr2.2:65609147-65609166 MS.gene96260:CDS 30.0%
TGATCAGATCTACTATCATT+CGG - chr2.2:65609672-65609691 MS.gene96260:CDS 30.0%
TTTATGATGTTTCTTGCATG+GGG - chr2.2:65610282-65610301 MS.gene96260:CDS 30.0%
TTTGTTGAAAGTGGAATTGT+TGG + chr2.2:65610855-65610874 None:intergenic 30.0%
! ATTCCAACTGCATCAAATTT+TGG - chr2.2:65608605-65608624 MS.gene96260:CDS 30.0%
! GTTATGAATTACATGGCTTT+TGG - chr2.2:65608830-65608849 MS.gene96260:CDS 30.0%
! GTTTTATGATGTTTCTTGCA+TGG - chr2.2:65610280-65610299 MS.gene96260:CDS 30.0%
! TTTGATAATTTCACCAATGG+AGG - chr2.2:65608470-65608489 MS.gene96260:CDS 30.0%
! TTTTCTTGCACTTCTTTTTG+CGG - chr2.2:65610413-65610432 MS.gene96260:CDS 30.0%
!! AGGTTTTGTCTAAGGATTTT+AGG - chr2.2:65608744-65608763 MS.gene96260:CDS 30.0%
!! ATGATACAAGGTTGATATAC+GGG - chr2.2:65609059-65609078 MS.gene96260:CDS 30.0%
!! CATTGATGATTGCTCTTTTA+GGG - chr2.2:65608385-65608404 MS.gene96260:CDS 30.0%
!! GGTTTTGTCTAAGGATTTTA+GGG - chr2.2:65608745-65608764 MS.gene96260:CDS 30.0%
!! TCAATTGAGATTGGTTTAGA+AGG - chr2.2:65608797-65608816 MS.gene96260:CDS 30.0%
!! TCATTGATGATTGCTCTTTT+AGG - chr2.2:65608384-65608403 MS.gene96260:CDS 30.0%
AACTGGGGTTATGAATTACA+TGG - chr2.2:65608823-65608842 MS.gene96260:CDS 35.0%
AAGGAGGATTCAATTGAGAT+TGG - chr2.2:65608788-65608807 MS.gene96260:CDS 35.0%
ACAGTTATACATATGTGGGA+TGG - chr2.2:65609890-65609909 MS.gene96260:CDS 35.0%
ACGTTGGAGTTTGAATTTGA+AGG - chr2.2:65608769-65608788 MS.gene96260:CDS 35.0%
ACTTAAAGTCATATGGGCAA+TGG - chr2.2:65610122-65610141 MS.gene96260:CDS 35.0%
AGAAAACGGCATGATTACAT+TGG - chr2.2:65610020-65610039 MS.gene96260:CDS 35.0%
AGGTACCTGAAACATTTGAA+GGG - chr2.2:65610336-65610355 MS.gene96260:CDS 35.0%
ATATAAGAGACCTTTGAGTC+AGG - chr2.2:65609111-65609130 MS.gene96260:CDS 35.0%
ATATCCCAAACAGAAAGAAC+AGG + chr2.2:65608587-65608606 None:intergenic 35.0%
ATGACTTTAAGTGGAGTTGT+GGG + chr2.2:65610114-65610133 None:intergenic 35.0%
ATTGCCCATATGACTTTAAG+TGG + chr2.2:65610123-65610142 None:intergenic 35.0%
CAACTCCACTTAAAGTCATA+TGG - chr2.2:65610115-65610134 MS.gene96260:CDS 35.0%
CAGTTATACATATGTGGGAT+GGG - chr2.2:65609891-65609910 MS.gene96260:CDS 35.0%
CATGTTTACTTGCAATACTC+AGG - chr2.2:65610378-65610397 MS.gene96260:CDS 35.0%
CTAACTGAGACAATTTATGC+TGG - chr2.2:65609580-65609599 MS.gene96260:CDS 35.0%
CTTAAAGTCATATGGGCAAT+GGG - chr2.2:65610123-65610142 MS.gene96260:CDS 35.0%
CTTTGACAATGTCCAAAAGA+AGG + chr2.2:65609794-65609813 None:intergenic 35.0%
GAATGATAGTAGATCTGATC+AGG + chr2.2:65609673-65609692 None:intergenic 35.0%
GCTTGTATAGAAAACGTAAC+CGG + chr2.2:65609496-65609515 None:intergenic 35.0%
GGATATTTGGAAGAGGAAAT+TGG - chr2.2:65610787-65610806 MS.gene96260:CDS 35.0%
GTGAGATGCAAAACAGAAAA+CGG - chr2.2:65610006-65610025 MS.gene96260:CDS 35.0%
GTTAACACTTACTGGATTGA+TGG - chr2.2:65609937-65609956 MS.gene96260:CDS 35.0%
TAAAGAATCTTGGCAGGATT+TGG + chr2.2:65609427-65609446 None:intergenic 35.0%
TAATGCGGTAAAACAGTATC+CGG + chr2.2:65609214-65609233 None:intergenic 35.0%
TATCACCCTTCAAATGTTTC+AGG + chr2.2:65610344-65610363 None:intergenic 35.0%
TATCCCAAACAGAAAGAACA+GGG + chr2.2:65608586-65608605 None:intergenic 35.0%
TATGACTTTAAGTGGAGTTG+TGG + chr2.2:65610115-65610134 None:intergenic 35.0%
TCACTTGGGCAATGATAATT+TGG - chr2.2:65610700-65610719 MS.gene96260:CDS 35.0%
TCCAGTTAGAACCTTTAAAC+AGG - chr2.2:65610907-65610926 MS.gene96260:CDS 35.0%
TCGGTGTATGAGAAGAAAAT+GGG - chr2.2:65609691-65609710 MS.gene96260:CDS 35.0%
TCTTTATCACCAGCATCTAA+AGG + chr2.2:65609325-65609344 None:intergenic 35.0%
TGAAACAATGAATCCTCCAT+TGG + chr2.2:65608486-65608505 None:intergenic 35.0%
TGACCAAAATTTGATGCAGT+TGG + chr2.2:65608611-65608630 None:intergenic 35.0%
TGATTCGATATATGAAGGTC+CGG - chr2.2:65609018-65609037 MS.gene96260:CDS 35.0%
TGTATGAGAAGAAAATGGGT+TGG - chr2.2:65609695-65609714 MS.gene96260:CDS 35.0%
TGTGTGAAGAATAGTGAAGA+TGG - chr2.2:65608974-65608993 MS.gene96260:CDS 35.0%
TTGATGTAAAGAATCTTGGC+AGG + chr2.2:65609433-65609452 None:intergenic 35.0%
TTGGAGTTTGAATTTGAAGG+AGG - chr2.2:65608772-65608791 MS.gene96260:CDS 35.0%
TTTAGATGCTGGTGATAAAG+AGG - chr2.2:65609324-65609343 MS.gene96260:CDS 35.0%
TTTCGAATCCGATTTCACTA+TGG - chr2.2:65608331-65608350 MS.gene96260:CDS 35.0%
! ACCTGTTTAAAGGTTCTAAC+TGG + chr2.2:65610911-65610930 None:intergenic 35.0%
! ATCAACAGGTTACCTTCTTT+TGG - chr2.2:65609779-65609798 MS.gene96260:CDS 35.0%
! CTTCTTTTGGACATTGTCAA+AGG - chr2.2:65609792-65609811 MS.gene96260:CDS 35.0%
! GGCTAACATGACTGTTATTT+GGG - chr2.2:65609168-65609187 MS.gene96260:CDS 35.0%
! GTATAACAACAGTGTTGTTC+TGG - chr2.2:65609753-65609772 MS.gene96260:CDS 35.0%
! GTTACGTTTTCTATACAAGC+CGG - chr2.2:65609496-65609515 MS.gene96260:CDS 35.0%
! TGAATTACATGGCTTTTGGT+TGG - chr2.2:65608834-65608853 MS.gene96260:CDS 35.0%
! TGTCCTGATTCGATATATGA+AGG - chr2.2:65609013-65609032 MS.gene96260:CDS 35.0%
! TTACGTTTTCTATACAAGCC+GGG - chr2.2:65609497-65609516 MS.gene96260:CDS 35.0%
! TTCCTATCCTTGTTCGTTTT+CGG - chr2.2:65608260-65608279 MS.gene96260:CDS 35.0%
! TTCGGTGTATGAGAAGAAAA+TGG - chr2.2:65609690-65609709 MS.gene96260:CDS 35.0%
! TTTACTGGCATGAAGCATTT+GGG - chr2.2:65610645-65610664 MS.gene96260:CDS 35.0%
!! GAATTACATGGCTTTTGGTT+GGG - chr2.2:65608835-65608854 MS.gene96260:CDS 35.0%
!! GTTTGAGACTTTTGGTCATT+TGG - chr2.2:65609261-65609280 MS.gene96260:CDS 35.0%
!! TATGCTTTGGAAAACGTTCA+TGG - chr2.2:65610675-65610694 MS.gene96260:CDS 35.0%
!! TTGGTTTGGTGAATGATACA+AGG - chr2.2:65609047-65609066 MS.gene96260:CDS 35.0%
!! TTTTACTGGCATGAAGCATT+TGG - chr2.2:65610644-65610663 MS.gene96260:CDS 35.0%
AAAACGTTCATGGACTCACT+TGG - chr2.2:65610685-65610704 MS.gene96260:CDS 40.0%
AAACGTTCATGGACTCACTT+GGG - chr2.2:65610686-65610705 MS.gene96260:CDS 40.0%
AAATATCACCACGAAGAACG+AGG - chr2.2:65608643-65608662 MS.gene96260:CDS 40.0%
AAGAGTGGTTATCTTGCCAT+AGG - chr2.2:65609847-65609866 MS.gene96260:CDS 40.0%
AATATCACCACGAAGAACGA+GGG - chr2.2:65608644-65608663 MS.gene96260:CDS 40.0%
ACTGAGACAATTTATGCTGG+TGG - chr2.2:65609583-65609602 MS.gene96260:CDS 40.0%
ATCAGCACCAATCATAAGCT+CGG + chr2.2:65608882-65608901 None:intergenic 40.0%
ATCCGAAAACGAACAAGGAT+AGG + chr2.2:65608265-65608284 None:intergenic 40.0%
ATCGGATTCGAAATCGATGA+AGG + chr2.2:65608324-65608343 None:intergenic 40.0%
ATGGTTCAACATCAACTCAG+AGG - chr2.2:65608350-65608369 MS.gene96260:CDS 40.0%
ATTACTACAGATCCCATTGG+TGG - chr2.2:65609538-65609557 MS.gene96260:CDS 40.0%
ATTGGAATTTACACGTCCCT+TGG - chr2.2:65610038-65610057 MS.gene96260:CDS 40.0%
CAGGTACCTGAAACATTTGA+AGG - chr2.2:65610335-65610354 MS.gene96260:CDS 40.0%
CATTATCTTCCTCAGAACCA+TGG - chr2.2:65609229-65609248 MS.gene96260:CDS 40.0%
CGGCTAACATGACTGTTATT+TGG - chr2.2:65609167-65609186 MS.gene96260:CDS 40.0%
CTACTACAAGATGGAACCAA+GGG + chr2.2:65610057-65610076 None:intergenic 40.0%
CTTGGTTCCATCTTGTAGTA+GGG - chr2.2:65610056-65610075 MS.gene96260:CDS 40.0%
GAAGGAGAAGGAGAAGAATA+AGG - chr2.2:65608682-65608701 MS.gene96260:CDS 40.0%
GATCCCTGTTCTTTCTGTTT+GGG - chr2.2:65608580-65608599 MS.gene96260:CDS 40.0%
GGACCTTCATATATCGAATC+AGG + chr2.2:65609019-65609038 None:intergenic 40.0%
GGTTATCTTGCCATAGGATT+TGG - chr2.2:65609853-65609872 MS.gene96260:CDS 40.0%
TCAATATCTATTGCTGCTCG+TGG - chr2.2:65609817-65609836 MS.gene96260:CDS 40.0%
TGATCCCTGTTCTTTCTGTT+TGG - chr2.2:65608579-65608598 MS.gene96260:CDS 40.0%
TGCATATCCGAAAACGAACA+AGG + chr2.2:65608270-65608289 None:intergenic 40.0%
TGCTGATGCAAAAGTTCCAT+TGG - chr2.2:65609363-65609382 MS.gene96260:CDS 40.0%
TGCTGCAATGCCAACAAAAA+TGG + chr2.2:65610622-65610641 None:intergenic 40.0%
TGTAAAGGTCCTTTAGATGC+TGG - chr2.2:65609313-65609332 MS.gene96260:CDS 40.0%
TGTTGGACTCAAGAGATCAA+TGG + chr2.2:65610838-65610857 None:intergenic 40.0%
TTACCATCAACCTGACTCAA+AGG + chr2.2:65609124-65609143 None:intergenic 40.0%
TTACTACAGATCCCATTGGT+GGG - chr2.2:65609539-65609558 MS.gene96260:CDS 40.0%
TTCACCAAACCAACTCTATC+CGG + chr2.2:65609040-65609059 None:intergenic 40.0%
TTCGAGCATACATCTTACAC+AGG - chr2.2:65609969-65609988 MS.gene96260:CDS 40.0%
TTGGTCGTGTTAACACTTAC+TGG - chr2.2:65609929-65609948 MS.gene96260:CDS 40.0%
TTGGTTCCATCTTGTAGTAG+GGG - chr2.2:65610057-65610076 MS.gene96260:CDS 40.0%
TTTGGGAGACAGATATGCTT+TGG - chr2.2:65610662-65610681 MS.gene96260:CDS 40.0%
! ACTGATTCCGAGCTTATGAT+TGG - chr2.2:65608872-65608891 MS.gene96260:CDS 40.0%
! ATGACTGTTATTTGGGCGTT+GGG - chr2.2:65609175-65609194 MS.gene96260:CDS 40.0%
! ATTGGTTTAGAAGGTGCAAC+TGG - chr2.2:65608806-65608825 MS.gene96260:CDS 40.0%
! CTTCAAATGTTTCAGGTACC+TGG + chr2.2:65610337-65610356 None:intergenic 40.0%
! GAACTGTTTCGAGTGCATTT+CGG + chr2.2:65608302-65608321 None:intergenic 40.0%
! TGTTGTTCTGGATGATCAAC+AGG - chr2.2:65609765-65609784 MS.gene96260:CDS 40.0%
! TTGGTTTAGAAGGTGCAACT+GGG - chr2.2:65608807-65608826 MS.gene96260:CDS 40.0%
!! TGGTTCTGAGGAAGATAATG+CGG + chr2.2:65609229-65609248 None:intergenic 40.0%
!!! CTAAGGATTTTAGGGTACGT+TGG - chr2.2:65608753-65608772 MS.gene96260:CDS 40.0%
!!! GTTACGGGTTTTAAGGAAGA+CGG - chr2.2:65608908-65608927 MS.gene96260:CDS 40.0%
!!! TTACGGGTTTTAAGGAAGAC+GGG - chr2.2:65608909-65608928 MS.gene96260:CDS 40.0%
AAATTGGGTGCTTGGCAATG+AGG - chr2.2:65610803-65610822 MS.gene96260:CDS 45.0%
AACCAGCTAATGCATTCCTC+AGG - chr2.2:65610514-65610533 MS.gene96260:CDS 45.0%
AATTTATGCTGGTGGTCCTG+AGG - chr2.2:65609591-65609610 MS.gene96260:CDS 45.0%
ACCAGCTAATGCATTCCTCA+GGG - chr2.2:65610515-65610534 MS.gene96260:CDS 45.0%
AGAATCTTGGCAGGATTTGG+CGG + chr2.2:65609424-65609443 None:intergenic 45.0%
AGCTCGGAATCAGTAGCATT+AGG + chr2.2:65608866-65608885 None:intergenic 45.0%
AGGGTCCCAAAACCATCAAA+TGG - chr2.2:65610534-65610553 MS.gene96260:CDS 45.0%
AGTGAAGATGGATCTGTCGA+AGG - chr2.2:65608986-65609005 MS.gene96260:CDS 45.0%
ATAAAGCGCAACATCATGCC+CGG + chr2.2:65609518-65609537 None:intergenic 45.0%
ATACTTGTTCAGCTGATGCG+TGG - chr2.2:65610210-65610229 MS.gene96260:CDS 45.0%
ATATACGGGCATAGGAGTGA+TGG - chr2.2:65609073-65609092 MS.gene96260:CDS 45.0%
CATCTTGTAGTAGGGGCAAA+AGG - chr2.2:65610064-65610083 MS.gene96260:CDS 45.0%
CATTCCACTGCCAAATCCTA+TGG + chr2.2:65609866-65609885 None:intergenic 45.0%
CCTACTACAAGATGGAACCA+AGG + chr2.2:65610058-65610077 None:intergenic 45.0%
CCTTGGTTCCATCTTGTAGT+AGG - chr2.2:65610055-65610074 MS.gene96260:CDS 45.0%
CGCATCAGCTGAACAAGTAT+AGG + chr2.2:65610210-65610229 None:intergenic 45.0%
CTCAGTTAGATTCCTCAGTG+TGG + chr2.2:65609569-65609588 None:intergenic 45.0%
GCTCGGAATCAGTAGCATTA+GGG + chr2.2:65608865-65608884 None:intergenic 45.0%
GGGTCCCAAAACCATCAAAT+GGG - chr2.2:65610535-65610554 MS.gene96260:CDS 45.0%
GGTCCCAAAACCATCAAATG+GGG - chr2.2:65610536-65610555 MS.gene96260:CDS 45.0%
GTGAAGATGGATCTGTCGAA+GGG - chr2.2:65608987-65609006 MS.gene96260:CDS 45.0%
TATACGGGCATAGGAGTGAT+GGG - chr2.2:65609074-65609093 MS.gene96260:CDS 45.0%
TATCTTCCTCAGAACCATGG+CGG - chr2.2:65609232-65609251 MS.gene96260:CDS 45.0%
TATGAAGGTCCGGATAGAGT+TGG - chr2.2:65609028-65609047 MS.gene96260:CDS 45.0%
TATGGGCAATGGGTAGTAGA+TGG - chr2.2:65610133-65610152 MS.gene96260:CDS 45.0%
TGAAGATGGATCTGTCGAAG+GGG - chr2.2:65608988-65609007 MS.gene96260:CDS 45.0%
TTCACAGGCTTCTGCAAGAA+TGG - chr2.2:65610881-65610900 MS.gene96260:CDS 45.0%
! AACTAGTTTGGGCTCCTGAT+AGG - chr2.2:65609641-65609660 MS.gene96260:CDS 45.0%
! ATTGGTTGTTTCCACTGGTC+CGG - chr2.2:65609381-65609400 MS.gene96260:CDS 45.0%
! CACTTACTGGATTGATGGAC+AGG - chr2.2:65609942-65609961 MS.gene96260:CDS 45.0%
! CATGACTGTTATTTGGGCGT+TGG - chr2.2:65609174-65609193 MS.gene96260:CDS 45.0%
! CTTTTGCCCCTACTACAAGA+TGG + chr2.2:65610066-65610085 None:intergenic 45.0%
! GTTCCATTGGTTGTTTCCAC+TGG - chr2.2:65609376-65609395 MS.gene96260:CDS 45.0%
! TAGTCCCACGGAACTAGTTT+GGG - chr2.2:65609630-65609649 MS.gene96260:CDS 45.0%
! TGACTGTTATTTGGGCGTTG+GGG - chr2.2:65609176-65609195 MS.gene96260:CDS 45.0%
! TGGTTTAGAAGGTGCAACTG+GGG - chr2.2:65608808-65608827 MS.gene96260:CDS 45.0%
!! AGACCTTTGAGTCAGGTTGA+TGG - chr2.2:65609118-65609137 MS.gene96260:CDS 45.0%
!! CAAGGTTGATATACGGGCAT+AGG - chr2.2:65609065-65609084 MS.gene96260:CDS 45.0%
!! GAAGAAAATGGGTTGGAGAG+TGG - chr2.2:65609702-65609721 MS.gene96260:CDS 45.0%
!! GCAGGATTTGCTTCCAGTTT+TGG - chr2.2:65610248-65610267 MS.gene96260:CDS 45.0%
!! GCTCCCCATTTGATGGTTTT+GGG + chr2.2:65610542-65610561 None:intergenic 45.0%
!! GCTTATGATTGGTGCTGATG+TGG - chr2.2:65608883-65608902 MS.gene96260:CDS 45.0%
!! GGCATTGCAGCACTTTTTAC+TGG - chr2.2:65610630-65610649 MS.gene96260:CDS 45.0%
!! GGTCTTCCGTTTGAGACTTT+TGG - chr2.2:65609253-65609272 MS.gene96260:CDS 45.0%
!! TATCTGGCTCTGATGTGCAT+TGG - chr2.2:65608426-65608445 MS.gene96260:CDS 45.0%
!! TATGATTGGTGCTGATGTGG+CGG - chr2.2:65608886-65608905 MS.gene96260:CDS 45.0%
!! TGATCAGGAGTGTTCCTATC+AGG + chr2.2:65609658-65609677 None:intergenic 45.0%
!! TGCTCCCCATTTGATGGTTT+TGG + chr2.2:65610543-65610562 None:intergenic 45.0%
!! TGGGTTGATGACAATGGTGT+TGG - chr2.2:65609910-65609929 MS.gene96260:CDS 45.0%
!!! GGCAGATCTGCCATTTTTGT+TGG - chr2.2:65610609-65610628 MS.gene96260:CDS 45.0%
AAACCATGCACGGCCAAAAC+TGG + chr2.2:65610264-65610283 None:intergenic 50.0%
ACCCTGAGGAATGCATTAGC+TGG + chr2.2:65610519-65610538 None:intergenic 50.0%
AGATGGGAAGGAGAAGGAGA+AGG - chr2.2:65608670-65608689 MS.gene96260:CDS 50.0%
AGTGTGGCATTCCCACCAAT+GGG + chr2.2:65609553-65609572 None:intergenic 50.0%
ATACGGGCATAGGAGTGATG+GGG - chr2.2:65609075-65609094 MS.gene96260:CDS 50.0%
CCTCTTTCCACTCTCAACAC+AGG + chr2.2:65609469-65609488 None:intergenic 50.0%
CCTGTGTTGAGAGTGGAAAG+AGG - chr2.2:65609466-65609485 MS.gene96260:CDS 50.0%
CGGGATCGGATATTTGGAAG+AGG - chr2.2:65610780-65610799 MS.gene96260:CDS 50.0%
CTAGTCCCACGGAACTAGTT+TGG - chr2.2:65609629-65609648 MS.gene96260:CDS 50.0%
CTTGCCATAGGATTTGGCAG+TGG - chr2.2:65609859-65609878 MS.gene96260:CDS 50.0%
GAATCTTGGCAGGATTTGGC+GGG + chr2.2:65609423-65609442 None:intergenic 50.0%
GCTCGTGGTGAGAAAAAGAG+TGG - chr2.2:65609832-65609851 MS.gene96260:CDS 50.0%
GGAAGAGGAAATTGGGTGCT+TGG - chr2.2:65610795-65610814 MS.gene96260:CDS 50.0%
GGACCAGTGGAAACAACCAA+TGG + chr2.2:65609382-65609401 None:intergenic 50.0%
GGGAGCAGCCAACGTTTAAA+AGG - chr2.2:65610556-65610575 MS.gene96260:CDS 50.0%
GGGTTAGGGTAATGCAAAGC+CGG + chr2.2:65609403-65609422 None:intergenic 50.0%
! AGGTCCGGATAGAGTTGGTT+TGG - chr2.2:65609033-65609052 MS.gene96260:CDS 50.0%
! TAGGAGTGATGGGGTTTCGT+TGG - chr2.2:65609084-65609103 MS.gene96260:CDS 50.0%
! TGTGGCGGTTACGGGTTTTA+AGG - chr2.2:65608901-65608920 MS.gene96260:CDS 50.0%
! TTTCGAGTGCATTTCGGAGC+AGG + chr2.2:65608296-65608315 None:intergenic 50.0%
!! CTTGCTTCCTGGAGGTATCT+TGG - chr2.2:65610308-65610327 MS.gene96260:CDS 50.0%
!! GAAAATGGGTTGGAGAGTGG+AGG - chr2.2:65609705-65609724 MS.gene96260:CDS 50.0%
!! GTGGGATGGGTTGATGACAA+TGG - chr2.2:65609904-65609923 MS.gene96260:CDS 50.0%
!! TGGCTCTGATGTGCATTGGT+GGG - chr2.2:65608430-65608449 MS.gene96260:CDS 50.0%
!!! TTGATGGTTTTGGGACCCTG+AGG + chr2.2:65610533-65610552 None:intergenic 50.0%
ACGAAGAACGAGGGAGGAGA+TGG - chr2.2:65608653-65608672 MS.gene96260:CDS 55.0%
AGGAGCCCAAACTAGTTCCG+TGG + chr2.2:65609638-65609657 None:intergenic 55.0%
ATCACCACGAAGAACGAGGG+AGG - chr2.2:65608647-65608666 MS.gene96260:CDS 55.0%
ATCTCCTCCCTCGTTCTTCG+TGG + chr2.2:65608654-65608673 None:intergenic 55.0%
CAGTGTGGCATTCCCACCAA+TGG + chr2.2:65609554-65609573 None:intergenic 55.0%
CGAAGAACGAGGGAGGAGAT+GGG - chr2.2:65608654-65608673 MS.gene96260:CDS 55.0%
CGAGCACGGGATCGGATATT+TGG - chr2.2:65610774-65610793 MS.gene96260:CDS 55.0%
GGAAGCTCCTGTGTTGAGAG+TGG - chr2.2:65609459-65609478 MS.gene96260:CDS 55.0%
GGAGCCCAAACTAGTTCCGT+GGG + chr2.2:65609637-65609656 None:intergenic 55.0%
GTAATGCAAAGCCGGACCAG+TGG + chr2.2:65609395-65609414 None:intergenic 55.0%
GTTGGCTGCTCCCCATTTGA+TGG + chr2.2:65610549-65610568 None:intergenic 55.0%
GTTGGGGAAGATGAGAGCTC+CGG - chr2.2:65609192-65609211 MS.gene96260:CDS 55.0%
TCCTGAGGCTCATGGTGTTC+AGG - chr2.2:65609606-65609625 MS.gene96260:CDS 55.0%
TCTCAAACGGAAGACCGCCA+TGG + chr2.2:65609249-65609268 None:intergenic 55.0%
TGGAGAGTGGAGGTTGTGGA+CGG - chr2.2:65609715-65609734 MS.gene96260:CDS 55.0%
TTGGTGGGAATGCCACACTG+AGG - chr2.2:65609554-65609573 MS.gene96260:CDS 55.0%
TTTGCGGTTGCTGAGCTTCG+CGG - chr2.2:65610429-65610448 MS.gene96260:CDS 55.0%
! GAGAGTGGAAAGAGGAGTGC+CGG - chr2.2:65609474-65609493 MS.gene96260:CDS 55.0%
! GCATGGGGTATCTTGCTTCC+TGG - chr2.2:65610297-65610316 MS.gene96260:CDS 55.0%
! GGCTCTGATGTGCATTGGTG+GGG - chr2.2:65608431-65608450 MS.gene96260:CDS 55.0%
! TGGGGTATCTTGCTTCCTGG+AGG - chr2.2:65610300-65610319 MS.gene96260:CDS 55.0%
! TGGTGTTCAGGCTAGTCCCA+CGG - chr2.2:65609618-65609637 MS.gene96260:CDS 55.0%
! TGGTTCTGCAGAAGCAGAGC+AGG - chr2.2:65610230-65610249 MS.gene96260:CDS 55.0%
!! CTGGCTCTGATGTGCATTGG+TGG - chr2.2:65608429-65608448 MS.gene96260:CDS 55.0%
!! CTTCCAGTTTTGGCCGTGCA+TGG - chr2.2:65610258-65610277 MS.gene96260:CDS 55.0%
!! GGAAGACGGGTTGCCATTTG+TGG - chr2.2:65608922-65608941 MS.gene96260:CDS 55.0%
!! TGGCAGGATTTGGCGGGTTA+GGG + chr2.2:65609417-65609436 None:intergenic 55.0%
!! TGGTGCTGATGTGGCGGTTA+CGG - chr2.2:65608892-65608911 MS.gene96260:CDS 55.0%
!! TTGGCAGGATTTGGCGGGTT+AGG + chr2.2:65609418-65609437 None:intergenic 55.0%
AGAAGCAGCGAGCACGGGAT+CGG - chr2.2:65610766-65610785 MS.gene96260:CDS 60.0%
AGAGTGGAGGTTGTGGACGG+GGG - chr2.2:65609718-65609737 MS.gene96260:CDS 60.0%
CTGGCTGCCAAGATACCTCC+AGG + chr2.2:65610318-65610337 None:intergenic 60.0%
GAACGAGGGAGGAGATGGGA+AGG - chr2.2:65608658-65608677 MS.gene96260:CDS 60.0%
GAGAGTGGAGGTTGTGGACG+GGG - chr2.2:65609717-65609736 MS.gene96260:CDS 60.0%
GCCTGAACACCATGAGCCTC+AGG + chr2.2:65609610-65609629 None:intergenic 60.0%
GGAGAGTGGAGGTTGTGGAC+GGG - chr2.2:65609716-65609735 MS.gene96260:CDS 60.0%
GGGAGGAGATGGGAAGGAGA+AGG - chr2.2:65608664-65608683 MS.gene96260:CDS 60.0%
GGGTTGGAGAGTGGAGGTTG+TGG - chr2.2:65609711-65609730 MS.gene96260:CDS 60.0%
! GGAAGACCGCCATGGTTCTG+AGG + chr2.2:65609241-65609260 None:intergenic 60.0%
!! CTGGAGGTATCTTGGCAGCC+AGG - chr2.2:65610316-65610335 MS.gene96260:CDS 60.0%
!! GGTGCTGATGTGGCGGTTAC+GGG - chr2.2:65608893-65608912 MS.gene96260:CDS 60.0%
ACCGCGAGAAGCAGCGAGCA+CGG - chr2.2:65610760-65610779 MS.gene96260:CDS 65.0%
GCTGGTGGTCCTGAGGCTCA+TGG - chr2.2:65609598-65609617 MS.gene96260:CDS 65.0%
CCCGTGCTCGCTGCTTCTCG+CGG + chr2.2:65610764-65610783 None:intergenic 70.0%
CCGCGAGAAGCAGCGAGCAC+GGG - chr2.2:65610761-65610780 MS.gene96260:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 65608236 65610932 65608236 ID=MS.gene96260
chr2.2 mRNA 65608236 65610932 65608236 ID=MS.gene96260.t1;Parent=MS.gene96260
chr2.2 exon 65608236 65610932 65608236 ID=MS.gene96260.t1.exon1;Parent=MS.gene96260.t1
chr2.2 CDS 65608236 65610932 65608236 ID=cds.MS.gene96260.t1;Parent=MS.gene96260.t1
Gene Sequence

>MS.gene96260

ATGCTTCGTGAAACTTTATTGATTTTCCTATCCTTGTTCGTTTTCGGATATGCAGATCCTGCTCCGAAATGCACTCGAAACAGTTCCTTCATCGATTTCGAATCCGATTTCACTATGGTTCAACATCAACTCAGAGGCCATTTCAAAATCATTGATGATTGCTCTTTTAGGGTTTCTCAATTTGATATGTTATCTGGCTCTGATGTGCATTGGTGGGGTGCAATTGATACAGATTTTGATAATTTCACCAATGGAGGATTCATTGTTTCAGATCATAAACTTAATCATACTTACGCCAATTTAACATTCGTTGTTCAATTGATGAAGAATGTTACTTGGGATATGATCCCTGTTCTTTCTGTTTGGGATATTCCAACTGCATCAAATTTTGGTCATGTTTTGATTCAAAATATCACCACGAAGAACGAGGGAGGAGATGGGAAGGAGAAGGAGAAGGAGAAGAATAAGGTAAGTGTTCATACTGAGCCAACTATGTTTGATAATTGTAAGGTTTTGTCTAAGGATTTTAGGGTACGTTGGAGTTTGAATTTGAAGGAGGATTCAATTGAGATTGGTTTAGAAGGTGCAACTGGGGTTATGAATTACATGGCTTTTGGTTGGGCTAACCCTAATGCTACTGATTCCGAGCTTATGATTGGTGCTGATGTGGCGGTTACGGGTTTTAAGGAAGACGGGTTGCCATTTGTGGATGATTTTTTTATTACTAAGTATAGTGAGTGTGTGAAGAATAGTGAAGATGGATCTGTCGAAGGGGTTTGTCCTGATTCGATATATGAAGGTCCGGATAGAGTTGGTTTGGTGAATGATACAAGGTTGATATACGGGCATAGGAGTGATGGGGTTTCGTTGGTTAGATATAAGAGACCTTTGAGTCAGGTTGATGGTAAATATGATCAATCTGTTGTTCAATCGGCTAACATGACTGTTATTTGGGCGTTGGGGAAGATGAGAGCTCCGGATACTGTTTTACCGCATTATCTTCCTCAGAACCATGGCGGTCTTCCGTTTGAGACTTTTGGTCATTTGGTTCTCAATGTTTCTCAGAATGTGAATGATTGTAAAGGTCCTTTAGATGCTGGTGATAAAGAGGATCAAGATGTGATCATTGCTGATGCAAAAGTTCCATTGGTTGTTTCCACTGGTCCGGCTTTGCATTACCCTAACCCGCCAAATCCTGCCAAGATTCTTTACATCAACAAAAAGGAAGCTCCTGTGTTGAGAGTGGAAAGAGGAGTGCCGGTTACGTTTTCTATACAAGCCGGGCATGATGTTGCGCTTTATATTACTACAGATCCCATTGGTGGGAATGCCACACTGAGGAATCTAACTGAGACAATTTATGCTGGTGGTCCTGAGGCTCATGGTGTTCAGGCTAGTCCCACGGAACTAGTTTGGGCTCCTGATAGGAACACTCCTGATCAGATCTACTATCATTCGGTGTATGAGAAGAAAATGGGTTGGAGAGTGGAGGTTGTGGACGGGGGTCTGTCCGACATGTATAACAACAGTGTTGTTCTGGATGATCAACAGGTTACCTTCTTTTGGACATTGTCAAAGGATTCAATATCTATTGCTGCTCGTGGTGAGAAAAAGAGTGGTTATCTTGCCATAGGATTTGGCAGTGGAATGATAAACAGTTATACATATGTGGGATGGGTTGATGACAATGGTGTTGGTCGTGTTAACACTTACTGGATTGATGGACAGGATGCTTCGAGCATACATCTTACACAGGAGAATCTCACACATGTGAGATGCAAAACAGAAAACGGCATGATTACATTGGAATTTACACGTCCCTTGGTTCCATCTTGTAGTAGGGGCAAAAGGCCAGAATGTAACAACATCATTGATCCCACAACTCCACTTAAAGTCATATGGGCAATGGGTAGTAGATGGAGTAATGAACATCTTACTGAAAGGAATATGCATACTGTAACTAGTAGCAGACCTATACTTGTTCAGCTGATGCGTGGTTCTGCAGAAGCAGAGCAGGATTTGCTTCCAGTTTTGGCCGTGCATGGTTTTATGATGTTTCTTGCATGGGGTATCTTGCTTCCTGGAGGTATCTTGGCAGCCAGGTACCTGAAACATTTGAAGGGTGATAATTGGTACAAAATTCATGTTTACTTGCAATACTCAGGGCTAGCAATAATTTTTCTTGCACTTCTTTTTGCGGTTGCTGAGCTTCGCGGTTTTCATGTTAGCTCAACACATGTTAAATTTGGAATTGCTGCAATAGTTTTAGCTTGCATACAACCAGCTAATGCATTCCTCAGGGTCCCAAAACCATCAAATGGGGAGCAGCCAACGTTTAAAAGGATAATTTGGGAATATTTGCACATAATTGTTGGCAGATCTGCCATTTTTGTTGGCATTGCAGCACTTTTTACTGGCATGAAGCATTTGGGAGACAGATATGCTTTGGAAAACGTTCATGGACTCACTTGGGCAATGATAATTTGGTTCTTAGTAGGTGCATTGAGTATTGCTTATTTCGAATACCGCGAGAAGCAGCGAGCACGGGATCGGATATTTGGAAGAGGAAATTGGGTGCTTGGCAATGAGGAAGATGATTCCATTGATCTCTTGAGTCCAACAATTCCACTTTCAACAAATAAAGATTCACAGGCTTCTGCAAGAATGGAAGTCCAGTTAGAACCTTTAAACAGGTAG
Protein sequence

>MS.gene96260.t1

MLRETLLIFLSLFVFGYADPAPKCTRNSSFIDFESDFTMVQHQLRGHFKIIDDCSFRVSQFDMLSGSDVHWWGAIDTDFDNFTNGGFIVSDHKLNHTYANLTFVVQLMKNVTWDMIPVLSVWDIPTASNFGHVLIQNITTKNEGGDGKEKEKEKNKVSVHTEPTMFDNCKVLSKDFRVRWSLNLKEDSIEIGLEGATGVMNYMAFGWANPNATDSELMIGADVAVTGFKEDGLPFVDDFFITKYSECVKNSEDGSVEGVCPDSIYEGPDRVGLVNDTRLIYGHRSDGVSLVRYKRPLSQVDGKYDQSVVQSANMTVIWALGKMRAPDTVLPHYLPQNHGGLPFETFGHLVLNVSQNVNDCKGPLDAGDKEDQDVIIADAKVPLVVSTGPALHYPNPPNPAKILYINKKEAPVLRVERGVPVTFSIQAGHDVALYITTDPIGGNATLRNLTETIYAGGPEAHGVQASPTELVWAPDRNTPDQIYYHSVYEKKMGWRVEVVDGGLSDMYNNSVVLDDQQVTFFWTLSKDSISIAARGEKKSGYLAIGFGSGMINSYTYVGWVDDNGVGRVNTYWIDGQDASSIHLTQENLTHVRCKTENGMITLEFTRPLVPSCSRGKRPECNNIIDPTTPLKVIWAMGSRWSNEHLTERNMHTVTSSRPILVQLMRGSAEAEQDLLPVLAVHGFMMFLAWGILLPGGILAARYLKHLKGDNWYKIHVYLQYSGLAIIFLALLFAVAELRGFHVSSTHVKFGIAAIVLACIQPANAFLRVPKPSNGEQPTFKRIIWEYLHIIVGRSAIFVGIAALFTGMKHLGDRYALENVHGLTWAMIIWFLVGALSIAYFEYREKQRARDRIFGRGNWVLGNEEDDSIDLLSPTIPLSTNKDSQASARMEVQLEPLNR