Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96507.t1 | RHN79300.1 | 45.5 | 189 | 93 | 1 | 7 | 185 | 27 | 215 | 1.20E-19 | 106.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96507.t1 | A0A072VJF9 | 45.5 | 189 | 93 | 1 | 7 | 185 | 62 | 250 | 8.6e-20 | 106.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049563 | MS.gene96507 | 0.816742 | 4.73E-52 | -1.69E-46 |
| MS.gene050139 | MS.gene96507 | 0.825334 | 4.96E-54 | -1.69E-46 |
| MS.gene051626 | MS.gene96507 | 0.821334 | 4.27E-53 | -1.69E-46 |
| MS.gene051789 | MS.gene96507 | 0.80022 | 1.60E-48 | -1.69E-46 |
| MS.gene053074 | MS.gene96507 | 0.83096 | 2.19E-55 | -1.69E-46 |
| MS.gene053184 | MS.gene96507 | 0.802388 | 5.75E-49 | -1.69E-46 |
| MS.gene054184 | MS.gene96507 | 0.81345 | 2.54E-51 | -1.69E-46 |
| MS.gene054185 | MS.gene96507 | 0.821738 | 3.44E-53 | -1.69E-46 |
| MS.gene054596 | MS.gene96507 | 0.804741 | 1.87E-49 | -1.69E-46 |
| MS.gene055359 | MS.gene96507 | 0.805411 | 1.35E-49 | -1.69E-46 |
| MS.gene058168 | MS.gene96507 | 0.844597 | 6.85E-59 | -1.69E-46 |
| MS.gene059006 | MS.gene96507 | 0.805349 | 1.39E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96507.t1 | MTR_1g054385 | 47.368 | 190 | 90 | 1 | 6 | 185 | 61 | 250 | 2.32e-48 | 158 |
| MS.gene96507.t1 | MTR_1g054380 | 40.196 | 204 | 72 | 6 | 1 | 169 | 1 | 189 | 9.83e-30 | 109 |
| MS.gene96507.t1 | MTR_5g048290 | 34.826 | 201 | 93 | 5 | 21 | 186 | 85 | 282 | 4.42e-21 | 87.8 |
| MS.gene96507.t1 | MTR_1g054370 | 32.353 | 204 | 97 | 5 | 21 | 186 | 90 | 290 | 5.87e-19 | 82.4 |
| MS.gene96507.t1 | MTR_1g054360 | 31.373 | 204 | 99 | 4 | 21 | 186 | 119 | 319 | 1.62e-12 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAACTTGGTCAAGGTTTA+AGG | 0.142695 | 1.4:+44268244 | None:intergenic |
| CTTGTAAACAATTCCTTCTT+TGG | 0.221022 | 1.4:+44268386 | None:intergenic |
| CTTCGAAAAGAGTGGATTAA+TGG | 0.276341 | 1.4:-44268463 | MS.gene96507:CDS |
| GGAGCAAAAGGTTTGTGATT+TGG | 0.304158 | 1.4:-44268592 | MS.gene96507:CDS |
| CTTGGTCAAGGTTTAAGGTT+AGG | 0.388940 | 1.4:+44268249 | None:intergenic |
| ATGGCTACTCTACATCAATC+TGG | 0.390287 | 1.4:-44268731 | MS.gene96507:CDS |
| GCAAGCAACACCCGTTCAAT+TGG | 0.411385 | 1.4:-44268634 | MS.gene96507:CDS |
| TGCTCGTTGTATGGAGCAAA+AGG | 0.418843 | 1.4:-44268604 | MS.gene96507:CDS |
| GGAAAATTGCACACCAAAGA+AGG | 0.418990 | 1.4:-44268399 | MS.gene96507:CDS |
| GGTCTCGATTGCTCGTTGTA+TGG | 0.434380 | 1.4:-44268613 | MS.gene96507:CDS |
| AGAAGGAATTGTTTACAAGT+TGG | 0.457133 | 1.4:-44268382 | MS.gene96507:CDS |
| GTGATTTGGAAAAGAGCTGC+AGG | 0.479576 | 1.4:-44268578 | MS.gene96507:CDS |
| AGGAATTGTTTACAAGTTGG+AGG | 0.487776 | 1.4:-44268379 | MS.gene96507:CDS |
| AAAGGATCATGAGGCATATG+AGG | 0.490646 | 1.4:-44268688 | MS.gene96507:CDS |
| GAGTGGATTAATGGAAAGAG+AGG | 0.504421 | 1.4:-44268454 | MS.gene96507:CDS |
| CTGAATCTGAGAATAACATA+GGG | 0.510118 | 1.4:-44268515 | MS.gene96507:CDS |
| TCTGAATCTGAGAATAACAT+AGG | 0.510558 | 1.4:-44268516 | MS.gene96507:CDS |
| TCATGAGGCATATGAGGAGT+GGG | 0.527499 | 1.4:-44268682 | MS.gene96507:CDS |
| GCAATCGAGACCAATTGAAC+GGG | 0.527802 | 1.4:+44268624 | None:intergenic |
| GTAGAGTAGCCATTCTCAGC+TGG | 0.533250 | 1.4:+44268741 | None:intergenic |
| AAGACTACACCAGCTGAGAA+TGG | 0.552919 | 1.4:-44268750 | MS.gene96507:CDS |
| GCACAATGTTGGCTTTAATG+AGG | 0.553089 | 1.4:-44268298 | MS.gene96507:CDS |
| TGGCTACTCTACATCAATCT+GGG | 0.557094 | 1.4:-44268730 | MS.gene96507:CDS |
| ATTGTAGTGCAGCACAATGT+TGG | 0.569055 | 1.4:-44268309 | MS.gene96507:CDS |
| GAATTAATCTTCGAAAAGAG+TGG | 0.570774 | 1.4:-44268471 | MS.gene96507:CDS |
| ATCATGAGGCATATGAGGAG+TGG | 0.581486 | 1.4:-44268683 | MS.gene96507:CDS |
| AAGAGCTGCAGGGCTCACAT+TGG | 0.582753 | 1.4:-44268567 | MS.gene96507:CDS |
| AGAATGGATCCCAAGAGATG+TGG | 0.586826 | 1.4:-44268795 | MS.gene96507:CDS |
| CGGGTGTTGCTTGCAAAACA+CGG | 0.601763 | 1.4:+44268643 | None:intergenic |
| TATCACAGAAAAGGATCATG+AGG | 0.608121 | 1.4:-44268697 | MS.gene96507:CDS |
| AGCAATCGAGACCAATTGAA+CGG | 0.624783 | 1.4:+44268623 | None:intergenic |
| TGATTTGGAAAAGAGCTGCA+GGG | 0.627589 | 1.4:-44268577 | MS.gene96507:CDS |
| CATGAGGCATATGAGGAGTG+GGG | 0.686751 | 1.4:-44268681 | MS.gene96507:CDS |
| TGAATCTGAGAATAACATAG+GGG | 0.713948 | 1.4:-44268514 | MS.gene96507:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAAAACTCCAAGATTTT+AGG | - | chr1.4:44268626-44268645 | MS.gene96507:CDS | 25.0% |
| !! | TGACAATTTTATCACAGAAA+AGG | - | chr1.4:44268340-44268359 | MS.gene96507:CDS | 25.0% |
| AGAAGGAATTGTTTACAAGT+TGG | - | chr1.4:44268664-44268683 | MS.gene96507:CDS | 30.0% | |
| CTGAATCTGAGAATAACATA+GGG | - | chr1.4:44268531-44268550 | MS.gene96507:CDS | 30.0% | |
| CTTGTAAACAATTCCTTCTT+TGG | + | chr1.4:44268663-44268682 | None:intergenic | 30.0% | |
| GAATTAATCTTCGAAAAGAG+TGG | - | chr1.4:44268575-44268594 | MS.gene96507:CDS | 30.0% | |
| TCTGAATCTGAGAATAACAT+AGG | - | chr1.4:44268530-44268549 | MS.gene96507:CDS | 30.0% | |
| TGAATCTGAGAATAACATAG+GGG | - | chr1.4:44268532-44268551 | MS.gene96507:CDS | 30.0% | |
| ! | GTGCAATTTTCCTAAAATCT+TGG | + | chr1.4:44268639-44268658 | None:intergenic | 30.0% |
| TATCACAGAAAAGGATCATG+AGG | - | chr1.4:44268349-44268368 | MS.gene96507:CDS | 35.0% | |
| ! | AGGAATTGTTTACAAGTTGG+AGG | - | chr1.4:44268667-44268686 | MS.gene96507:CDS | 35.0% |
| !! | CTTCGAAAAGAGTGGATTAA+TGG | - | chr1.4:44268583-44268602 | MS.gene96507:CDS | 35.0% |
| AAAGGATCATGAGGCATATG+AGG | - | chr1.4:44268358-44268377 | MS.gene96507:CDS | 40.0% | |
| AGCAATCGAGACCAATTGAA+CGG | + | chr1.4:44268426-44268445 | None:intergenic | 40.0% | |
| ATGGCTACTCTACATCAATC+TGG | - | chr1.4:44268315-44268334 | MS.gene96507:CDS | 40.0% | |
| ATTGTAGTGCAGCACAATGT+TGG | - | chr1.4:44268737-44268756 | MS.gene96507:CDS | 40.0% | |
| GGAAAATTGCACACCAAAGA+AGG | - | chr1.4:44268647-44268666 | MS.gene96507:CDS | 40.0% | |
| GGAGCAAAAGGTTTGTGATT+TGG | - | chr1.4:44268454-44268473 | MS.gene96507:CDS | 40.0% | |
| TGGCTACTCTACATCAATCT+GGG | - | chr1.4:44268316-44268335 | MS.gene96507:CDS | 40.0% | |
| ! | CTACGTTTTCCACATCTCTT+GGG | + | chr1.4:44268263-44268282 | None:intergenic | 40.0% |
| ! | TGATTTGGAAAAGAGCTGCA+GGG | - | chr1.4:44268469-44268488 | MS.gene96507:CDS | 40.0% |
| !! | GAGTGGATTAATGGAAAGAG+AGG | - | chr1.4:44268592-44268611 | MS.gene96507:CDS | 40.0% |
| !! | GCACAATGTTGGCTTTAATG+AGG | - | chr1.4:44268748-44268767 | MS.gene96507:CDS | 40.0% |
| AAGACTACACCAGCTGAGAA+TGG | - | chr1.4:44268296-44268315 | MS.gene96507:CDS | 45.0% | |
| ATCATGAGGCATATGAGGAG+TGG | - | chr1.4:44268363-44268382 | MS.gene96507:CDS | 45.0% | |
| GCAATCGAGACCAATTGAAC+GGG | + | chr1.4:44268425-44268444 | None:intergenic | 45.0% | |
| TCATGAGGCATATGAGGAGT+GGG | - | chr1.4:44268364-44268383 | MS.gene96507:CDS | 45.0% | |
| TGCTCGTTGTATGGAGCAAA+AGG | - | chr1.4:44268442-44268461 | MS.gene96507:CDS | 45.0% | |
| ! | GCTACGTTTTCCACATCTCT+TGG | + | chr1.4:44268264-44268283 | None:intergenic | 45.0% |
| ! | GTGATTTGGAAAAGAGCTGC+AGG | - | chr1.4:44268468-44268487 | MS.gene96507:CDS | 45.0% |
| CATGAGGCATATGAGGAGTG+GGG | - | chr1.4:44268365-44268384 | MS.gene96507:CDS | 50.0% | |
| GCAAGCAACACCCGTTCAAT+TGG | - | chr1.4:44268412-44268431 | MS.gene96507:CDS | 50.0% | |
| GGTCTCGATTGCTCGTTGTA+TGG | - | chr1.4:44268433-44268452 | MS.gene96507:CDS | 50.0% | |
| GTAGAGTAGCCATTCTCAGC+TGG | + | chr1.4:44268308-44268327 | None:intergenic | 50.0% | |
| !! | CGGGTGTTGCTTGCAAAACA+CGG | + | chr1.4:44268406-44268425 | None:intergenic | 50.0% |
| AAGAGCTGCAGGGCTCACAT+TGG | - | chr1.4:44268479-44268498 | MS.gene96507:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 44268254 | 44268814 | 44268254 | ID=MS.gene96507 |
| chr1.4 | mRNA | 44268254 | 44268814 | 44268254 | ID=MS.gene96507.t1;Parent=MS.gene96507 |
| chr1.4 | exon | 44268254 | 44268814 | 44268254 | ID=MS.gene96507.t1.exon1;Parent=MS.gene96507.t1 |
| chr1.4 | CDS | 44268254 | 44268814 | 44268254 | ID=cds.MS.gene96507.t1;Parent=MS.gene96507.t1 |
| Gene Sequence |
| Protein sequence |
