Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96771.t1 | XP_013456765.1 | 93.6 | 94 | 6 | 0 | 7 | 100 | 67 | 160 | 1.70E-38 | 168.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96771.t1 | A0A072UN48 | 93.6 | 94 | 6 | 0 | 7 | 100 | 67 | 160 | 1.2e-38 | 168.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050404 | MS.gene96771 | 0.84543 | 4.08E-59 | -1.69E-46 |
| MS.gene050406 | MS.gene96771 | 0.822959 | 1.79E-53 | -1.69E-46 |
| MS.gene051171 | MS.gene96771 | 0.831706 | 1.43E-55 | -1.69E-46 |
| MS.gene051242 | MS.gene96771 | 0.851365 | 9.31E-61 | -1.69E-46 |
| MS.gene051244 | MS.gene96771 | 0.847678 | 9.93E-60 | -1.69E-46 |
| MS.gene052290 | MS.gene96771 | 0.809244 | 2.08E-50 | -1.69E-46 |
| MS.gene053330 | MS.gene96771 | 0.819924 | 8.99E-53 | -1.69E-46 |
| MS.gene053971 | MS.gene96771 | 0.850499 | 1.63E-60 | -1.69E-46 |
| MS.gene055370 | MS.gene96771 | 0.811975 | 5.35E-51 | -1.69E-46 |
| MS.gene056633 | MS.gene96771 | 0.864858 | 8.98E-65 | -1.69E-46 |
| MS.gene056664 | MS.gene96771 | 0.908599 | 1.43E-81 | -1.69E-46 |
| MS.gene058233 | MS.gene96771 | 0.832573 | 8.75E-56 | -1.69E-46 |
| MS.gene058276 | MS.gene96771 | 0.838035 | 3.65E-57 | -1.69E-46 |
| MS.gene058746 | MS.gene96771 | 0.881476 | 2.32E-70 | -1.69E-46 |
| MS.gene059894 | MS.gene96771 | 0.809629 | 1.72E-50 | -1.69E-46 |
| MS.gene060407 | MS.gene96771 | 0.816359 | 5.76E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96771.t1 | MTR_4g082330 | 92.157 | 102 | 8 | 0 | 1 | 102 | 61 | 162 | 3.74e-51 | 160 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 48 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTGTGAGGGTGGCTTCTA+AGG | 0.266560 | 4.3:+28092252 | MS.gene96771:CDS |
| ACCGCGTTGCAGCCCGCGTC+GGG | 0.276137 | 4.3:-28092168 | None:intergenic |
| GGCTGCAACGCGGTTGGATT+GGG | 0.277741 | 4.3:+28092177 | MS.gene96771:CDS |
| TCGTCGCGGCGTAGGGGCTT+TGG | 0.318358 | 4.3:+28092519 | MS.gene96771:intron |
| CGTCGCGGCGTAGGGGCTTT+GGG | 0.319742 | 4.3:+28092520 | MS.gene96771:intron |
| GGGCTGCAACGCGGTTGGAT+TGG | 0.330424 | 4.3:+28092176 | MS.gene96771:CDS |
| TATTAAGTTTGTATGTAGTT+TGG | 0.346486 | 4.3:-28092590 | None:intergenic |
| TGGTCAATTACGTGCGTGGC+TGG | 0.395524 | 4.3:+28092307 | MS.gene96771:CDS |
| ATTCCGAACCGTCCACCTTC+CGG | 0.399012 | 4.3:-28092381 | None:intergenic |
| TCGATAAAAGGGACGCCGTT+AGG | 0.414254 | 4.3:+28092567 | MS.gene96771:CDS |
| CGTAACTATGCAATGTGTGC+GGG | 0.429868 | 4.3:+28092277 | MS.gene96771:CDS |
| ACGGTTCGGAATATCTTCTT+AGG | 0.438601 | 4.3:+28092392 | MS.gene96771:CDS |
| TCGCCGGAAGGTGGACGGTT+CGG | 0.463920 | 4.3:+28092378 | MS.gene96771:CDS |
| AACCGCGTTGCAGCCCGCGT+CGG | 0.468668 | 4.3:-28092169 | None:intergenic |
| TGTAGCTGTGACGGCGGTGT+TGG | 0.471963 | 4.3:+28092333 | MS.gene96771:CDS |
| CGGCGGTGTTGGTTTACGTC+GGG | 0.474783 | 4.3:+28092344 | MS.gene96771:CDS |
| GACGCGGGCTGCAACGCGGT+TGG | 0.485105 | 4.3:+28092171 | MS.gene96771:CDS |
| TGGTTTACGTCGGGATTCAA+AGG | 0.489575 | 4.3:+28092353 | MS.gene96771:CDS |
| AACGGAACTTGACGCCGTCA+TGG | 0.509958 | 4.3:-28092125 | None:intergenic |
| TTCACCTTCCTCGCCACCAA+CGG | 0.518986 | 4.3:-28092143 | None:intergenic |
| TGAAATGAATAAGATGTTGA+AGG | 0.521333 | 4.3:+28092204 | MS.gene96771:CDS |
| TCGGGATTCAAAGGCGTCGC+CGG | 0.522229 | 4.3:+28092362 | MS.gene96771:CDS |
| ACGGCGTCAAGTTCCGTTGG+TGG | 0.523122 | 4.3:+28092130 | MS.gene96771:CDS |
| ACGGAACTTGACGCCGTCAT+GGG | 0.524511 | 4.3:-28092124 | None:intergenic |
| AGTTCCGTTGGTGGCGAGGA+AGG | 0.531811 | 4.3:+28092139 | MS.gene96771:CDS |
| GTCAAGTTCCGTTGGTGGCG+AGG | 0.531974 | 4.3:+28092135 | MS.gene96771:CDS |
| TTGTATGTAGTTTGGCCTAA+CGG | 0.534335 | 4.3:-28092582 | None:intergenic |
| CCGCGTTGCAGCCCGCGTCG+GGG | 0.537525 | 4.3:-28092167 | None:intergenic |
| TCAAAGGCGTCGCCGGAAGG+TGG | 0.547716 | 4.3:+28092369 | MS.gene96771:CDS |
| CCCCGACGCGGGCTGCAACG+CGG | 0.549132 | 4.3:+28092167 | MS.gene96771:CDS |
| TAAGATGTTGAAGGTGCGGA+TGG | 0.561256 | 4.3:+28092213 | MS.gene96771:CDS |
| GGAAGGTGAAGCCCCGACGC+GGG | 0.567663 | 4.3:+28092156 | MS.gene96771:CDS |
| GGCTGGAGCTGTAGCTGTGA+CGG | 0.568693 | 4.3:+28092324 | MS.gene96771:CDS |
| ATGACGGCGTCAAGTTCCGT+TGG | 0.572657 | 4.3:+28092127 | MS.gene96771:CDS |
| GATTCAAAGGCGTCGCCGGA+AGG | 0.574232 | 4.3:+28092366 | MS.gene96771:CDS |
| ACGGCGGTGTTGGTTTACGT+CGG | 0.576717 | 4.3:+28092343 | MS.gene96771:CDS |
| AGGCGTCGCCGGAAGGTGGA+CGG | 0.577749 | 4.3:+28092373 | MS.gene96771:CDS |
| GTAACTATGCAATGTGTGCG+GGG | 0.581011 | 4.3:+28092278 | MS.gene96771:CDS |
| GATGTTGAAGGTGCGGATGG+AGG | 0.600823 | 4.3:+28092216 | MS.gene96771:CDS |
| GCGGATGGAGGCTATGAGAG+TGG | 0.619387 | 4.3:+28092228 | MS.gene96771:CDS |
| TCGTAACTATGCAATGTGTG+CGG | 0.627278 | 4.3:+28092276 | MS.gene96771:CDS |
| TGGAGCTGTAGCTGTGACGG+CGG | 0.631922 | 4.3:+28092327 | MS.gene96771:CDS |
| TAACTATGCAATGTGTGCGG+GGG | 0.646393 | 4.3:+28092279 | MS.gene96771:CDS |
| TGAATAAGATGTTGAAGGTG+CGG | 0.652058 | 4.3:+28092209 | MS.gene96771:CDS |
| AGGAAGGTGAAGCCCCGACG+CGG | 0.653291 | 4.3:+28092155 | MS.gene96771:CDS |
| CTATGAGAGTGGAAATTGTG+AGG | 0.658550 | 4.3:+28092239 | MS.gene96771:CDS |
| GAGAGTGGAAATTGTGAGGG+TGG | 0.678186 | 4.3:+28092243 | MS.gene96771:CDS |
| TATGAGAGTGGAAATTGTGA+GGG | 0.682385 | 4.3:+28092240 | MS.gene96771:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAAGAAATAATAATTTC+CGG | - | chr4.3:28092445-28092464 | None:intergenic | 15.0% |
| !!! | TTTTTTTCTCTTCGATAAAA+GGG | + | chr4.3:28092556-28092575 | MS.gene96771:CDS | 20.0% |
| ! | GATCAGAATTCTAATTACTA+TGG | - | chr4.3:28092479-28092498 | None:intergenic | 25.0% |
| ! | TGAAATGAATAAGATGTTGA+AGG | + | chr4.3:28092204-28092223 | MS.gene96771:CDS | 25.0% |
| !! | CTTTTATCGAAGAGAAAAAA+AGG | - | chr4.3:28092557-28092576 | None:intergenic | 25.0% |
| !!! | CTTTTTTTCTCTTCGATAAA+AGG | + | chr4.3:28092555-28092574 | MS.gene96771:CDS | 25.0% |
| AAAAAGATGAGGTGAGTATA+TGG | + | chr4.3:28092416-28092435 | MS.gene96771:intron | 30.0% | |
| TATGAGAGTGGAAATTGTGA+GGG | + | chr4.3:28092240-28092259 | MS.gene96771:CDS | 35.0% | |
| TCTTCTTAGGCAAAAAGATG+AGG | + | chr4.3:28092405-28092424 | MS.gene96771:CDS | 35.0% | |
| TGAATAAGATGTTGAAGGTG+CGG | + | chr4.3:28092209-28092228 | MS.gene96771:CDS | 35.0% | |
| TTGTATGTAGTTTGGCCTAA+CGG | - | chr4.3:28092585-28092604 | None:intergenic | 35.0% | |
| CTATGAGAGTGGAAATTGTG+AGG | + | chr4.3:28092239-28092258 | MS.gene96771:CDS | 40.0% | |
| TCGTAACTATGCAATGTGTG+CGG | + | chr4.3:28092276-28092295 | MS.gene96771:CDS | 40.0% | |
| ! | ACGGTTCGGAATATCTTCTT+AGG | + | chr4.3:28092392-28092411 | MS.gene96771:CDS | 40.0% |
| AATTGTGAGGGTGGCTTCTA+AGG | + | chr4.3:28092252-28092271 | MS.gene96771:CDS | 45.0% | |
| CGTAACTATGCAATGTGTGC+GGG | + | chr4.3:28092277-28092296 | MS.gene96771:CDS | 45.0% | |
| GTAACTATGCAATGTGTGCG+GGG | + | chr4.3:28092278-28092297 | MS.gene96771:CDS | 45.0% | |
| TAACTATGCAATGTGTGCGG+GGG | + | chr4.3:28092279-28092298 | MS.gene96771:CDS | 45.0% | |
| TGAGGTGAGTATATGGCATC+CGG | + | chr4.3:28092423-28092442 | MS.gene96771:intron | 45.0% | |
| TGGTTTACGTCGGGATTCAA+AGG | + | chr4.3:28092353-28092372 | MS.gene96771:CDS | 45.0% | |
| ! | AGATGTCCTAAGTATCGTCG+CGG | + | chr4.3:28092505-28092524 | MS.gene96771:intron | 45.0% |
| ! | TAAGATGTTGAAGGTGCGGA+TGG | + | chr4.3:28092213-28092232 | MS.gene96771:CDS | 45.0% |
| !! | GTTTTGGTCAATTACGTGCG+TGG | + | chr4.3:28092303-28092322 | MS.gene96771:CDS | 45.0% |
| GAGAGTGGAAATTGTGAGGG+TGG | + | chr4.3:28092243-28092262 | MS.gene96771:CDS | 50.0% | |
| TCGATAAAAGGGACGCCGTT+AGG | + | chr4.3:28092567-28092586 | MS.gene96771:CDS | 50.0% | |
| ATGACGGCGTCAAGTTCCGT+TGG | + | chr4.3:28092127-28092146 | MS.gene96771:CDS | 55.0% | |
| ATTCCGAACCGTCCACCTTC+CGG | - | chr4.3:28092384-28092403 | None:intergenic | 55.0% | |
| TGGTCAATTACGTGCGTGGC+TGG | + | chr4.3:28092307-28092326 | MS.gene96771:CDS | 55.0% | |
| TTCACCTTCCTCGCCACCAA+CGG | - | chr4.3:28092146-28092165 | None:intergenic | 55.0% | |
| ! | CTAAGTATCGTCGCGGCGTA+GGG | + | chr4.3:28092512-28092531 | MS.gene96771:intron | 55.0% |
| ! | GATGTTGAAGGTGCGGATGG+AGG | + | chr4.3:28092216-28092235 | MS.gene96771:CDS | 55.0% |
| ! | TAAGTATCGTCGCGGCGTAG+GGG | + | chr4.3:28092513-28092532 | MS.gene96771:intron | 55.0% |
| !! | ACGGCGGTGTTGGTTTACGT+CGG | + | chr4.3:28092343-28092362 | MS.gene96771:CDS | 55.0% |
| ACGGCGTCAAGTTCCGTTGG+TGG | + | chr4.3:28092130-28092149 | MS.gene96771:CDS | 60.0% | |
| CCTACGCCGCGACGATACTT+AGG | - | chr4.3:28092514-28092533 | None:intergenic | 60.0% | |
| GCGGATGGAGGCTATGAGAG+TGG | + | chr4.3:28092228-28092247 | MS.gene96771:CDS | 60.0% | |
| GGCTGCAACGCGGTTGGATT+GGG | + | chr4.3:28092177-28092196 | MS.gene96771:CDS | 60.0% | |
| GGCTGGAGCTGTAGCTGTGA+CGG | + | chr4.3:28092324-28092343 | MS.gene96771:CDS | 60.0% | |
| TGGAGCTGTAGCTGTGACGG+CGG | + | chr4.3:28092327-28092346 | MS.gene96771:CDS | 60.0% | |
| TGTAGCTGTGACGGCGGTGT+TGG | + | chr4.3:28092333-28092352 | MS.gene96771:CDS | 60.0% | |
| ! | CCTAAGTATCGTCGCGGCGT+AGG | + | chr4.3:28092511-28092530 | MS.gene96771:intron | 60.0% |
| !! | AGTTCCGTTGGTGGCGAGGA+AGG | + | chr4.3:28092139-28092158 | MS.gene96771:CDS | 60.0% |
| !! | CGGCGGTGTTGGTTTACGTC+GGG | + | chr4.3:28092344-28092363 | MS.gene96771:CDS | 60.0% |
| !! | GATTCAAAGGCGTCGCCGGA+AGG | + | chr4.3:28092366-28092385 | MS.gene96771:CDS | 60.0% |
| !! | GTCAAGTTCCGTTGGTGGCG+AGG | + | chr4.3:28092135-28092154 | MS.gene96771:CDS | 60.0% |
| !! | TCGGGATTCAAAGGCGTCGC+CGG | + | chr4.3:28092362-28092381 | MS.gene96771:CDS | 60.0% |
| !!! | CAATGTGTGCGGGGGCGTTT+TGG | + | chr4.3:28092287-28092306 | MS.gene96771:CDS | 60.0% |
| AGGAAGGTGAAGCCCCGACG+CGG | + | chr4.3:28092155-28092174 | MS.gene96771:CDS | 65.0% | |
| GGGCTGCAACGCGGTTGGAT+TGG | + | chr4.3:28092176-28092195 | MS.gene96771:CDS | 65.0% | |
| TCGCCGGAAGGTGGACGGTT+CGG | + | chr4.3:28092378-28092397 | MS.gene96771:CDS | 65.0% | |
| !! | TCAAAGGCGTCGCCGGAAGG+TGG | + | chr4.3:28092369-28092388 | MS.gene96771:CDS | 65.0% |
| AACCGCGTTGCAGCCCGCGT+CGG | - | chr4.3:28092172-28092191 | None:intergenic | 70.0% | |
| CGTCGCGGCGTAGGGGCTTT+GGG | + | chr4.3:28092520-28092539 | MS.gene96771:intron | 70.0% | |
| GGAAGGTGAAGCCCCGACGC+GGG | + | chr4.3:28092156-28092175 | MS.gene96771:CDS | 70.0% | |
| TCGTCGCGGCGTAGGGGCTT+TGG | + | chr4.3:28092519-28092538 | MS.gene96771:intron | 70.0% | |
| ! | AGGCGTCGCCGGAAGGTGGA+CGG | + | chr4.3:28092373-28092392 | MS.gene96771:CDS | 70.0% |
| ACCGCGTTGCAGCCCGCGTC+GGG | - | chr4.3:28092171-28092190 | None:intergenic | 75.0% | |
| ! | GACGCGGGCTGCAACGCGGT+TGG | + | chr4.3:28092171-28092190 | MS.gene96771:CDS | 75.0% |
| ! | CCGCGTTGCAGCCCGCGTCG+GGG | - | chr4.3:28092170-28092189 | None:intergenic | 80.0% |
| !! | CCCCGACGCGGGCTGCAACG+CGG | + | chr4.3:28092167-28092186 | MS.gene96771:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 28092127 | 28092610 | 28092127 | ID=MS.gene96771 |
| chr4.3 | mRNA | 28092127 | 28092610 | 28092127 | ID=MS.gene96771.t1;Parent=MS.gene96771 |
| chr4.3 | exon | 28092127 | 28092426 | 28092127 | ID=MS.gene96771.t1.exon1;Parent=MS.gene96771.t1 |
| chr4.3 | CDS | 28092127 | 28092426 | 28092127 | ID=cds.MS.gene96771.t1;Parent=MS.gene96771.t1 |
| chr4.3 | exon | 28092533 | 28092610 | 28092533 | ID=MS.gene96771.t1.exon2;Parent=MS.gene96771.t1 |
| chr4.3 | CDS | 28092533 | 28092610 | 28092533 | ID=cds.MS.gene96771.t1;Parent=MS.gene96771.t1 |
| Gene Sequence |
| Protein sequence |
