AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene98479

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98479.t1 MTR_5g028510 69.085 317 91 3 107 422 18 328 5.81e-141 421
MS.gene98479.t1 MTR_5g028450 69.580 286 51 2 549 799 1 285 8.58e-134 401
MS.gene98479.t1 MTR_5g028190 53.757 346 115 5 491 799 185 522 2.94e-113 356
MS.gene98479.t1 MTR_5g028190 47.273 220 86 6 205 421 5 197 5.58e-44 167
MS.gene98479.t1 MTR_5g028930 47.514 362 151 4 474 799 248 606 2.02e-99 322
MS.gene98479.t1 MTR_5g028930 48.780 287 119 10 126 407 1 264 4.02e-61 218
MS.gene98479.t1 MTR_5g028130 48.640 331 124 5 508 798 1 325 6.85e-96 303
MS.gene98479.t1 MTR_5g028830 43.985 266 122 5 392 649 42 288 1.22e-57 199
MS.gene98479.t1 MTR_5g028690 43.939 198 74 3 645 806 36 232 1.30e-43 158
MS.gene98479.t1 MTR_3g055310 33.865 251 163 2 507 756 14 262 2.12e-42 158
MS.gene98479.t1 MTR_2g438740 34.241 257 160 2 552 807 279 527 3.20e-42 163
MS.gene98479.t1 MTR_3g055310 33.865 251 163 2 507 756 77 325 8.11e-42 158
MS.gene98479.t1 MTR_3g055310 33.865 251 163 2 507 756 129 377 2.99e-41 158
MS.gene98479.t1 MTR_3g055310 33.865 251 163 2 507 756 157 405 3.45e-41 158
MS.gene98479.t1 MTR_3g055310 33.865 251 163 2 507 756 155 403 4.69e-41 158
MS.gene98479.t1 MTR_3g455790 33.721 258 168 2 500 756 258 513 4.99e-41 160
MS.gene98479.t1 MTR_3g055310 33.865 251 163 2 507 756 182 430 5.94e-41 158
MS.gene98479.t1 MTR_5g028920 54.483 145 62 2 509 652 86 227 8.30e-41 150
MS.gene98479.t1 MTR_5g091220 26.034 580 328 11 219 787 69 558 2.67e-40 158
MS.gene98479.t1 MTR_3g452020 32.927 246 162 2 509 753 92 335 5.06e-37 145
MS.gene98479.t1 MTR_2g438760 34.348 230 143 1 552 781 273 494 8.32e-37 147
MS.gene98479.t1 MTR_2g438440 38.095 210 129 1 547 756 394 602 1.75e-36 147
MS.gene98479.t1 MTR_3g452020 32.927 246 162 2 509 753 268 511 5.30e-36 145
MS.gene98479.t1 MTR_2g438700 31.104 299 186 6 547 842 397 678 2.84e-35 144
MS.gene98479.t1 MTR_2g438720 32.700 263 166 4 547 807 399 652 9.57e-35 142
MS.gene98479.t1 MTR_2g020450 26.789 601 335 19 217 756 42 598 1.02e-34 142
MS.gene98479.t1 MTR_2g438400 31.518 257 166 3 552 807 258 505 5.53e-34 138
MS.gene98479.t1 MTR_2g438640 36.667 210 132 1 547 756 399 607 2.58e-33 137
MS.gene98479.t1 MTR_6g017215 39.459 185 72 4 650 799 6 185 2.30e-31 123
MS.gene98479.t1 MTR_5g028840 38.421 190 77 4 650 800 2 190 5.60e-31 121
MS.gene98479.t1 MTR_2g020510 25.248 606 346 19 212 756 37 596 9.12e-31 129
MS.gene98479.t1 MTR_5g028110 39.683 189 56 4 652 805 4 169 1.65e-30 119
MS.gene98479.t1 MTR_5g028900 56.604 106 34 2 651 756 3 96 3.71e-30 117
MS.gene98479.t1 MTR_2g438640 34.286 210 111 2 547 756 399 581 7.51e-28 120
MS.gene98479.t1 MTR_3g058570 32.407 216 136 3 548 756 83 295 5.52e-26 111
MS.gene98479.t1 MTR_2g017930 36.406 217 128 4 542 756 352 560 1.91e-24 109
MS.gene98479.t1 MTR_1g113820 32.057 209 140 2 547 755 274 480 1.93e-24 109
MS.gene98479.t1 MTR_2g438470 41.353 133 77 1 594 726 366 497 2.40e-23 105
MS.gene98479.t1 MTR_2g020470 31.224 237 125 5 533 756 367 578 1.71e-22 103
MS.gene98479.t1 MTR_5g085340 28.767 219 139 3 549 756 443 655 2.42e-22 103
MS.gene98479.t1 MTR_5g085350 28.774 212 146 2 549 756 437 647 4.38e-22 102
MS.gene98479.t1 MTR_5g085350 28.774 212 146 2 549 756 424 634 4.46e-22 102
MS.gene98479.t1 MTR_5g085360 21.361 632 355 17 218 761 74 651 4.03e-20 96.3
MS.gene98479.t1 MTR_1g113760 29.358 218 148 3 540 754 231 445 7.08e-20 94.7
MS.gene98479.t1 MTR_3g050500 28.372 215 145 4 548 756 427 638 7.74e-20 95.5
MS.gene98479.t1 MTR_1g113730 27.315 216 155 2 540 755 37 250 8.68e-18 86.7
MS.gene98479.t1 MTR_3g051060 25.714 210 152 3 550 756 435 643 4.78e-16 83.2
MS.gene98479.t1 MTR_2g438640 39.252 107 64 1 650 756 399 504 2.80e-15 80.5
MS.gene98479.t1 MTR_2g438580 37.805 82 50 1 665 746 3 83 6.40e-12 62.4
MS.gene98479.t1 MTR_3g058550 28.829 222 106 8 214 420 6 190 5.42e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98479.t1 AT3G18670 41.985 262 150 2 496 756 295 555 7.95e-61 218
MS.gene98479.t1 AT3G18670 29.302 215 110 6 219 427 88 266 2.25e-16 84.0
MS.gene98479.t1 AT3G18670 42.800 250 141 2 508 756 252 500 1.66e-60 216
MS.gene98479.t1 AT3G18670 28.571 196 98 6 219 408 88 247 1.27e-11 68.6
MS.gene98479.t1 AT5G04700 28.939 622 306 20 219 798 118 645 1.16e-52 195
MS.gene98479.t1 AT5G04690 29.250 547 293 13 219 761 113 569 2.99e-51 191
MS.gene98479.t1 AT5G04690 28.702 547 282 14 219 761 113 555 1.72e-48 183
MS.gene98479.t1 AT5G04730 27.517 596 299 17 245 798 74 578 5.99e-48 181
MS.gene98479.t1 AT5G04680 27.943 637 330 21 219 798 105 669 2.59e-45 174
MS.gene98479.t1 AT5G04690 36.719 256 158 3 507 761 283 535 3.57e-42 164
MS.gene98479.t1 AT5G35810 37.383 214 128 3 549 756 35 248 3.97e-41 155
MS.gene98479.t1 AT5G04680 32.845 341 173 8 506 798 185 517 3.28e-39 154
MS.gene98479.t1 AT5G04680 32.845 341 173 8 506 798 294 626 6.82e-39 154
MS.gene98479.t1 AT5G04680 32.845 341 173 8 506 798 308 640 7.05e-39 154
MS.gene98479.t1 AT3G54065 44.697 132 67 1 636 761 8 139 1.67e-31 123
MS.gene98479.t1 AT5G04700 26.196 439 228 16 219 651 118 466 4.22e-20 95.1

Find 184 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTACTTTCATAAATAATT+TGG 0.159583 5.4:+19122364 None:intergenic
CTCTTGAATCTGGGTCATTT+TGG 0.169381 5.4:+19124776 None:intergenic
CATTATCGACATGATCTTAA+AGG 0.186021 5.4:-19118050 MS.gene98479:CDS
TTGTCCATCCTTCATCTTCT+TGG 0.196052 5.4:+19124885 None:intergenic
CAGAACAATTCTTTATTCTT+TGG 0.203069 5.4:+19123159 None:intergenic
GAGTATGATATTGCCGAAAT+TGG 0.213255 5.4:-19120960 MS.gene98479:CDS
ATCTGCAGGGGCAAGGTCTA+TGG 0.241341 5.4:-19124951 MS.gene98479:CDS
TCAACTGCTGAAAGAAAATT+AGG 0.246224 5.4:+19119244 None:intergenic
GTACTACAATGAACCACTTA+TGG 0.252983 5.4:+19118017 None:intergenic
TGTATCACTGCTGAAATATT+TGG 0.253668 5.4:-19122709 MS.gene98479:intron
AAGAGAGATTCAATGGTTTA+AGG 0.258197 5.4:-19119020 MS.gene98479:intron
TACCATGCACTTTGCTATTT+CGG 0.263241 5.4:+19122918 None:intergenic
TACTAACCTTGCTCCAAATC+TGG 0.275014 5.4:+19124748 None:intergenic
ATGTTAGGGCAAGGCCTTCT+AGG 0.297090 5.4:-19123487 MS.gene98479:intron
GCGCTTGTTGCACGTTATAC+TGG 0.297367 5.4:-19122946 MS.gene98479:CDS
TTTACTTACCATTACTATCT+AGG 0.299491 5.4:+19120390 None:intergenic
CAAAACTCGATTCTGTGAAT+TGG 0.302690 5.4:+19122744 None:intergenic
TTCAAGAGCTACCCCAGATT+TGG 0.304496 5.4:-19124761 MS.gene98479:CDS
GTTGTTCGAATAATGCTTTC+CGG 0.310295 5.4:-19120831 MS.gene98479:intron
AATCATTTAGGCTCATTATA+TGG 0.310793 5.4:-19123322 MS.gene98479:CDS
TCTGGGGTAGCTCTTGAATC+TGG 0.315237 5.4:+19124766 None:intergenic
GGCACCCAATTGGGATTTCT+AGG 0.327771 5.4:-19122387 MS.gene98479:CDS
TATATATGACATCGGCTTCC+AGG 0.333476 5.4:-19123086 MS.gene98479:CDS
TCGGACGAAGTTCCCAATTT+CGG 0.335617 5.4:+19120947 None:intergenic
CTCCGTCCCTACGTTGTTAA+GGG 0.336330 5.4:-19122523 MS.gene98479:CDS
GTGCATGGTAGAGGAAATAA+AGG 0.338215 5.4:-19122905 MS.gene98479:CDS
TAAATGATTCCAGGCGCTTT+TGG 0.340866 5.4:+19123336 None:intergenic
ATTCTTCTAGCTGCTGCTAA+TGG 0.346856 5.4:-19122832 MS.gene98479:CDS
CCGTGGAGTTGCATATTTGT+TGG 0.349278 5.4:-19120429 MS.gene98479:CDS
TTGCACCTGGCAGCACATTT+AGG 0.356259 5.4:-19119091 MS.gene98479:CDS
TGTGAGGATTTATATAGAAT+TGG 0.361764 5.4:-19117186 MS.gene98479:CDS
TCTCCGTCCCTACGTTGTTA+AGG 0.362809 5.4:-19122524 MS.gene98479:CDS
CATGTGCAAGGAGGACTTCA+TGG 0.362910 5.4:-19117248 MS.gene98479:CDS
ATTTATCAGGTAAGGATTGC+TGG 0.365909 5.4:+19122457 None:intergenic
CAAAGAATAAAGAATTGTTC+TGG 0.367690 5.4:-19123158 MS.gene98479:CDS
TACTACAATGAACCACTTAT+GGG 0.368135 5.4:+19118018 None:intergenic
TACACACATTATGAAGTTAT+AGG 0.373654 5.4:-19119403 MS.gene98479:CDS
ACTAAGGCCGAGAATTTATC+AGG 0.379921 5.4:+19122444 None:intergenic
TGATGCATCATGGAAAATAA+AGG 0.382865 5.4:-19123434 MS.gene98479:CDS
CTTTAAGATCATGTCGATAA+TGG 0.388292 5.4:+19118051 None:intergenic
TTTGGCCTAGAAATCCCAAT+TGG 0.395842 5.4:+19122382 None:intergenic
TTAGCAGCAGCTAGAAGAAT+AGG 0.397885 5.4:+19122835 None:intergenic
CTTCATGCTGCAGTAATTGC+TGG 0.399972 5.4:-19123054 MS.gene98479:CDS
TTTCCAAGTGGCACCCAATT+GGG 0.405636 5.4:-19122396 MS.gene98479:CDS
GCAACATCTTATACAATTGT+AGG 0.407639 5.4:-19118361 MS.gene98479:CDS
CTTCAATTATGTCAGGAATC+AGG 0.410277 5.4:-19119444 MS.gene98479:intron
GTCGAACACTTTGGATTTGG+TGG 0.412310 5.4:+19122771 None:intergenic
TTTGTCGAACACTTTGGATT+TGG 0.414542 5.4:+19122768 None:intergenic
GCTGCAGCCTTTACAGTGCC+AGG 0.415001 5.4:-19118313 MS.gene98479:CDS
TTTCAAATTCTGAAACAGTC+TGG 0.425070 5.4:+19123458 None:intergenic
CAGTCTGGATAACGCCTAGA+AGG 0.426486 5.4:+19123473 None:intergenic
GAACTCGATTATTCCGTGTT+GGG 0.428128 5.4:+19119291 None:intergenic
CTTTCAATGAATATCTCATA+AGG 0.433992 5.4:+19118430 None:intergenic
GGTGGAAACGACGACAAAAC+TGG 0.435847 5.4:-19118292 MS.gene98479:CDS
ATCCTTACCTGATAAATTCT+CGG 0.437868 5.4:-19122451 MS.gene98479:CDS
TAGTGAAAGATGGAGAGAAA+TGG 0.438852 5.4:-19118396 MS.gene98479:CDS
ATTATCGACATGATCTTAAA+GGG 0.440142 5.4:-19118049 MS.gene98479:CDS
TCCTCCTTGCACATGGAAAT+CGG 0.440630 5.4:+19117256 None:intergenic
GATATTACAAGAACGATAAT+CGG 0.441300 5.4:+19117971 None:intergenic
CAGCAACACATGTTACCTTT+CGG 0.444549 5.4:+19120928 None:intergenic
AGTATGATATTGCCGAAATT+GGG 0.448600 5.4:-19120959 MS.gene98479:CDS
TAATGAGCCTAAATGATTCC+AGG 0.455349 5.4:+19123327 None:intergenic
CCCAGAAACGTTGCTAGAAA+AGG 0.455513 5.4:-19122878 MS.gene98479:CDS
GTGATGAGAGGTACGCCTAT+TGG 0.457145 5.4:-19123132 MS.gene98479:CDS
GGTACGCCTATTGGAAATAT+CGG 0.457767 5.4:-19123123 MS.gene98479:CDS
TGCTGAATCTCGTACTGATC+AGG 0.459882 5.4:-19124911 MS.gene98479:CDS
AGTAGTATGATGAAATCAAC+AGG 0.460104 5.4:-19125051 None:intergenic
GGCCGAGAATTTATCAGGTA+AGG 0.460680 5.4:+19122449 None:intergenic
CAACTCATACATTCTGTCAA+TGG 0.460886 5.4:-19119163 MS.gene98479:CDS
TCGAACACTTTGGATTTGGT+GGG 0.466790 5.4:+19122772 None:intergenic
ACTAACCTTGCTCCAAATCT+GGG 0.469008 5.4:+19124749 None:intergenic
TATATTGATAATGATTGCTC+AGG 0.469018 5.4:+19122552 None:intergenic
TACCATTACTATCTAGGTAA+TGG 0.471379 5.4:+19120396 None:intergenic
TTAAAACAGCCAAAAGCGCC+TGG 0.474873 5.4:-19123345 MS.gene98479:CDS
AAAAGCGCCTGGAATCATTT+AGG 0.476909 5.4:-19123334 MS.gene98479:CDS
AAATGCAAAGAGAGATTCAA+TGG 0.476952 5.4:-19119027 MS.gene98479:CDS
ATTCTCGGCCTTAGTTGCAT+TGG 0.477418 5.4:-19122436 MS.gene98479:CDS
CATACGAAAATATGCCTCTC+TGG 0.481711 5.4:+19119215 None:intergenic
AGACATCGTATAGACTCGTT+TGG 0.481995 5.4:-19119124 MS.gene98479:CDS
CGAACACTTTGGATTTGGTG+GGG 0.482350 5.4:+19122773 None:intergenic
TTGCATTTGCAGAGCAGCAC+CGG 0.486301 5.4:+19119042 None:intergenic
TGTGGAAAATGTGGAATTGT+TGG 0.486891 5.4:-19123028 MS.gene98479:CDS
TAGCAGCAGCTAGAAGAATA+GGG 0.490849 5.4:+19122836 None:intergenic
ATTGGATTTGTCGAACACTT+TGG 0.491192 5.4:+19122762 None:intergenic
TGTTATGCAAAACTATCTGC+AGG 0.494906 5.4:-19124965 MS.gene98479:CDS
AGGACTTCATGGACCCAACT+TGG 0.496671 5.4:-19117237 MS.gene98479:CDS
AGTGAAAGATGGAGAGAAAT+GGG 0.498200 5.4:-19118395 MS.gene98479:CDS
CCTTTCTGCCTATGCCACAT+TGG 0.504501 5.4:+19120865 None:intergenic
CTAAAGACAACTATGAAAGA+TGG 0.509180 5.4:-19124993 MS.gene98479:CDS
TTCCATTACCTAGATAGTAA+TGG 0.509237 5.4:-19120398 MS.gene98479:intron
TCAACAGGTGTTGCCTCTGA+TGG 0.509585 5.4:-19125036 MS.gene98479:CDS
TGAACTCGATTATTCCGTGT+TGG 0.510589 5.4:+19119290 None:intergenic
AATCCCAATTGGGTGCCACT+TGG 0.512054 5.4:+19122393 None:intergenic
CTGGGGTAGCTCTTGAATCT+GGG 0.512944 5.4:+19124767 None:intergenic
ATACTCCCAAGTGCCATGAT+TGG 0.513042 5.4:+19117995 None:intergenic
CATCTTTCACTAGCTCCTCA+TGG 0.514505 5.4:+19118407 None:intergenic
CCGATGTGGTACTCCAAGTT+GGG 0.516225 5.4:+19117224 None:intergenic
TGCTGGAAATGTGGAAAATG+TGG 0.517608 5.4:-19123037 MS.gene98479:CDS
TATGGCAAAGATTTAACAGC+CGG 0.518073 5.4:-19123304 MS.gene98479:CDS
TATCGTTCTTGTAATATCAC+AGG 0.519358 5.4:-19117966 MS.gene98479:intron
AGTTACTTCTGCCGAAGTGT+TGG 0.521693 5.4:-19119373 MS.gene98479:CDS
TCTGCAGGGGCAAGGTCTAT+GGG 0.522236 5.4:-19124950 MS.gene98479:CDS
AATAGCAAAGTGCATGGTAG+AGG 0.523953 5.4:-19122914 MS.gene98479:CDS
TATGTTGTCATGTAAGAAGA+TGG 0.525735 5.4:+19118264 None:intergenic
TCTGATCGTCATAGCAGATC+CGG 0.526463 5.4:-19119061 MS.gene98479:CDS
TCAGGTAAGGATTGCTGGAG+AGG 0.527967 5.4:+19122462 None:intergenic
TCCGATGTGGTACTCCAAGT+TGG 0.528011 5.4:+19117223 None:intergenic
TGGCAATAATTTGTTGCACC+TGG 0.529393 5.4:-19119104 MS.gene98479:CDS
ATTCTTTATTCTTTGGAGAG+TGG 0.532153 5.4:+19123166 None:intergenic
GAACACTTTGGATTTGGTGG+GGG 0.542791 5.4:+19122774 None:intergenic
CATATTCACTGCATTTCTCA+TGG 0.544524 5.4:-19118245 MS.gene98479:CDS
AATTTGAAACTGATGCATCA+TGG 0.545997 5.4:-19123444 MS.gene98479:CDS
TGGAATTGTTGGTGAAAGTA+GGG 0.547426 5.4:-19123017 MS.gene98479:CDS
GTTTCCACCTGGCACTGTAA+AGG 0.547912 5.4:+19118306 None:intergenic
TCCATACCGATATTTCCAAT+AGG 0.548152 5.4:+19123117 None:intergenic
CCAATGTGGCATAGGCAGAA+AGG 0.549410 5.4:-19120865 MS.gene98479:CDS
CATGAGGAGCTAGTGAAAGA+TGG 0.552759 5.4:-19118406 MS.gene98479:CDS
TTGGCCTAGAAATCCCAATT+GGG 0.552989 5.4:+19122383 None:intergenic
CTGCTATGACGATCAGATGA+AGG 0.554930 5.4:+19119067 None:intergenic
AGCATGAAGAAGCGAAGACC+TGG 0.554946 5.4:+19123068 None:intergenic
GTTCATTGTAGTACCAATCA+TGG 0.555376 5.4:-19118008 MS.gene98479:CDS
AATTGGGAACTTCGTCCGAA+AGG 0.555672 5.4:-19120943 MS.gene98479:CDS
CATGAAGTCCTCCTTGCACA+TGG 0.555778 5.4:+19117249 None:intergenic
CGAGTTCATAAATGCCATGA+GGG 0.557678 5.4:-19119275 MS.gene98479:CDS
GCCTATTGGAAATATCGGTA+TGG 0.560203 5.4:-19123118 MS.gene98479:CDS
ATGATTCCCTTAACAACGTA+GGG 0.560792 5.4:+19122517 None:intergenic
ACCTTGTTCATGATCTGCAC+CGG 0.561022 5.4:+19123285 None:intergenic
TTGCAGATTTGAGCGTGGAA+AGG 0.566854 5.4:-19120995 MS.gene98479:intron
GCTACCCCAGATTTGGAGCA+AGG 0.566888 5.4:-19124754 MS.gene98479:intron
GTGCCACTTGGAAAAGATGT+AGG 0.572327 5.4:+19122405 None:intergenic
TAATAGTTCAAAGCTCAAAG+AGG 0.573010 5.4:-19119344 MS.gene98479:CDS
AAGGAAGCCGAAAATGATGA+TGG 0.573881 5.4:-19118460 MS.gene98479:CDS
CTACTTTGTAAGATATTCCG+TGG 0.578446 5.4:-19120446 MS.gene98479:CDS
AGGGATATGAAACCCATAAG+TGG 0.578644 5.4:-19118030 MS.gene98479:CDS
CAAAACTATCTGCAGGGGCA+AGG 0.578679 5.4:-19124958 MS.gene98479:CDS
GAATAAAGAATTGTTCTGGA+AGG 0.580301 5.4:-19123154 MS.gene98479:CDS
GTGGAATTGTTGGTGAAAGT+AGG 0.581941 5.4:-19123018 MS.gene98479:CDS
GTAGTACCAATCATGGCACT+TGG 0.582602 5.4:-19118001 MS.gene98479:CDS
TGCATGGTAGAGGAAATAAA+GGG 0.582858 5.4:-19122904 MS.gene98479:CDS
TCCGCAGTCCAATGTGGCAT+AGG 0.583947 5.4:-19120873 MS.gene98479:CDS
TCAGGCTTCCAAGAAGATGA+AGG 0.586986 5.4:-19124893 MS.gene98479:CDS
ATGTTGTCATGTAAGAAGAT+GGG 0.590707 5.4:+19118265 None:intergenic
TTCTATATAAATCCTCACAC+CGG 0.591273 5.4:+19117190 None:intergenic
ATGTGGCTTTGAGATTACTC+AGG 0.601489 5.4:-19122590 MS.gene98479:CDS
GCTTCCAAGAAGATGAAGGA+TGG 0.601738 5.4:-19124889 MS.gene98479:CDS
CATATCATTCAACTATCATG+TGG 0.604069 5.4:-19123397 MS.gene98479:CDS
AGATTGAACCTAACTCTGCA+TGG 0.604549 5.4:+19117145 None:intergenic
GAAGTCATGAACCCGGTGTG+AGG 0.610733 5.4:-19117202 MS.gene98479:CDS
TACCGAAATAGCAAAGTGCA+TGG 0.611483 5.4:-19122920 MS.gene98479:CDS
CTCGAATGTATATATGACAT+CGG 0.616856 5.4:-19123094 MS.gene98479:CDS
ACATTCTGTCAATGGACGGA+AGG 0.617474 5.4:-19119155 MS.gene98479:CDS
ATTAGGATTAGCATCCCTCA+TGG 0.622934 5.4:+19119261 None:intergenic
GCCTATGCCACATTGGACTG+CGG 0.623841 5.4:+19120872 None:intergenic
TAGTACCAATCATGGCACTT+GGG 0.623895 5.4:-19118000 MS.gene98479:CDS
GTTATGCAAAACTATCTGCA+GGG 0.624004 5.4:-19124964 MS.gene98479:CDS
GTTACCGATTTCCATGTGCA+AGG 0.624933 5.4:-19117260 MS.gene98479:CDS
TTCCCTTAACAACGTAGGGA+CGG 0.626973 5.4:+19122521 None:intergenic
GCAGTTGATAGCTGCCAGAG+AGG 0.630753 5.4:-19119229 MS.gene98479:CDS
TGCAGTAATTGCTGGAAATG+TGG 0.630821 5.4:-19123046 MS.gene98479:CDS
AAGGACCTAAATGTGCTGCC+AGG 0.631007 5.4:+19119086 None:intergenic
AATGATTCCCTTAACAACGT+AGG 0.635342 5.4:+19122516 None:intergenic
TCATACATTCTGTCAATGGA+CGG 0.635424 5.4:-19119159 MS.gene98479:CDS
CACATCGGAAGTCATGAACC+CGG 0.638087 5.4:-19117209 MS.gene98479:CDS
TTGATGAACTATTCCATCAG+AGG 0.645567 5.4:+19125023 None:intergenic
TTATGCAAAACTATCTGCAG+GGG 0.646152 5.4:-19124963 MS.gene98479:CDS
TCTATATAAATCCTCACACC+GGG 0.648350 5.4:+19117191 None:intergenic
CGGGTTCATGACTTCCGATG+TGG 0.648678 5.4:+19117210 None:intergenic
CCAACAAATATGCAACTCCA+CGG 0.652186 5.4:+19120429 None:intergenic
TTTCATTGCAGATTTGAGCG+TGG 0.657835 5.4:-19121000 MS.gene98479:intron
TATACCAATGAAAATCAAGA+CGG 0.658060 5.4:+19118189 None:intergenic
ATATGCATGCAAGACGAACA+CGG 0.664176 5.4:-19122982 MS.gene98479:CDS
ATGTTGCATGCATCCCAACA+CGG 0.665878 5.4:-19119304 MS.gene98479:CDS
TTGTTCTGGAAGGTGATGAG+AGG 0.672631 5.4:-19123144 MS.gene98479:CDS
AAAGGCTGCAGCAAACATGA+TGG 0.673254 5.4:+19118324 None:intergenic
GCCGGTGCAGATCATGAACA+AGG 0.680709 5.4:-19123286 MS.gene98479:intron
ATGCAACATTGCTTCATACG+CGG 0.681582 5.4:+19119318 None:intergenic
ACCGATTTCCATGTGCAAGG+AGG 0.683975 5.4:-19117257 MS.gene98479:CDS
CCCAACTTGGAGTACCACAT+CGG 0.685074 5.4:-19117224 MS.gene98479:CDS
CTAACCTTGCTCCAAATCTG+GGG 0.686888 5.4:+19124750 None:intergenic
TCGAGTTCATAAATGCCATG+AGG 0.690060 5.4:-19119276 MS.gene98479:CDS
GCAGCCTTTACAGTGCCAGG+TGG 0.698792 5.4:-19118310 MS.gene98479:CDS
ATTTCTTCCGCAGTCCAATG+TGG 0.705212 5.4:-19120879 MS.gene98479:CDS
GGCTGCAGCAAACATGATGG+TGG 0.726270 5.4:+19118327 None:intergenic
GATATTCATTGAAAGCCATG+AGG 0.729208 5.4:-19118422 MS.gene98479:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr5.4 gene 19117154 19125067 19117154 ID=MS.gene98479
chr5.4 mRNA 19117154 19125067 19117154 ID=MS.gene98479.t1;Parent=MS.gene98479
chr5.4 exon 19124755 19125067 19124755 ID=MS.gene98479.t1.exon1;Parent=MS.gene98479.t1
chr5.4 CDS 19124755 19125067 19124755 ID=cds.MS.gene98479.t1;Parent=MS.gene98479.t1
chr5.4 exon 19123287 19123502 19123287 ID=MS.gene98479.t1.exon2;Parent=MS.gene98479.t1
chr5.4 CDS 19123287 19123502 19123287 ID=cds.MS.gene98479.t1;Parent=MS.gene98479.t1
chr5.4 exon 19122710 19123201 19122710 ID=MS.gene98479.t1.exon3;Parent=MS.gene98479.t1
chr5.4 CDS 19122710 19123201 19122710 ID=cds.MS.gene98479.t1;Parent=MS.gene98479.t1
chr5.4 exon 19122370 19122612 19122370 ID=MS.gene98479.t1.exon4;Parent=MS.gene98479.t1
chr5.4 CDS 19122370 19122612 19122370 ID=cds.MS.gene98479.t1;Parent=MS.gene98479.t1
chr5.4 exon 19120832 19121011 19120832 ID=MS.gene98479.t1.exon5;Parent=MS.gene98479.t1
chr5.4 CDS 19120832 19121011 19120832 ID=cds.MS.gene98479.t1;Parent=MS.gene98479.t1
chr5.4 exon 19120399 19120473 19120399 ID=MS.gene98479.t1.exon6;Parent=MS.gene98479.t1
chr5.4 CDS 19120399 19120473 19120399 ID=cds.MS.gene98479.t1;Parent=MS.gene98479.t1
chr5.4 exon 19119021 19119451 19119021 ID=MS.gene98479.t1.exon7;Parent=MS.gene98479.t1
chr5.4 CDS 19119021 19119451 19119021 ID=cds.MS.gene98479.t1;Parent=MS.gene98479.t1
chr5.4 exon 19118194 19118512 19118194 ID=MS.gene98479.t1.exon8;Parent=MS.gene98479.t1
chr5.4 CDS 19118194 19118512 19118194 ID=cds.MS.gene98479.t1;Parent=MS.gene98479.t1
chr5.4 exon 19117967 19118088 19117967 ID=MS.gene98479.t1.exon9;Parent=MS.gene98479.t1
chr5.4 CDS 19117967 19118088 19117967 ID=cds.MS.gene98479.t1;Parent=MS.gene98479.t1
chr5.4 exon 19117154 19117294 19117154 ID=MS.gene98479.t1.exon10;Parent=MS.gene98479.t1
chr5.4 CDS 19117154 19117294 19117154 ID=cds.MS.gene98479.t1;Parent=MS.gene98479.t1
Gene Sequence

>MS.gene98479

ATGATGAAATCAACAGGTGTTGCCTCTGATGGAATAGTTCATCAAGTACTCACTAAAGACAACTATGAAAGATGGAAAATTGTTATGCAAAACTATCTGCAGGGGCAAGGTCTATGGGACGTTGTCATACAAAATGCTGAATCTCGTACTGATCAGGCTTCCAAGAAGATGAAGGATGGACAACAAGCTTTACACTTCTTTCAACTATCATCATGTAAACGAGATTTTCTTGAAACAGTCAAAAAATCAGGCTGGAATAACTTCACCAACCAAAATGACCCAGATTCAAGAGCTACCCCAGATTTGGAGCAAGGTTAGTATCTGTATACTCATATGTCATATCCATATTTTTTTAGTACAACTCCTCACTGTGATACTATTACTTGCTTTCTCTATTGTTTTATTAATCACGGCAATATCTAAGTTTATTTGTATATATTTTTGGTGGTGGCCGGGGTATAAACCTCGGACATTACATATATTATGCATTGTCCCTACCAACTAAGTTAAGTTAATCTATTTTTTCCAATAATGATATATATCTAGACACACCCAATTTTTCTACATCCATTCAACAATTCATTTTCTCTATATCTCTCTCTTCATAACACATCGTGAATCTATCACATTCACTCTCTTTTATCTTTATCTCTTTCAAATATACATTTGGAGTGTCTAAGTAACATTTTTTTTTTCTTCTTAAAATCAAATACTCTTCATTTATACTATATATAAAGAAAAGATGCTTAAAATACTTGAGTTTGAGCTAACTTTTTCACTACCTATTTTACCTATGATTTAAACTATTTTATTTACTATTTTATCCTTTAATAATATTATTTTAAATTTTAATTAAAAAATTTAAATCCCATTAAAATAGGAATTATCCCACTACAATAAGTACAATATTTAAACAAATTATCCTATATTAATGTTATATTGTTGGTGGTATTAAAAAAGATAAGAATTTATATTATATTTGTTAAATTATTGTTATTATTGAAAAAGATAAAGATGATTTTTTGGATTTGTTACAATTTAAAAGTCACAAATATTTGTACTTTTTTTTTTGACAAGAAATATTTGTACTTATAATTTGAATCAAAATTAAAATTTCATATAAAATACCATTCATAATTTACATGGGTCAGGGCAATAAAAAGAGCGGAAAGACGGGCACGTGAGTGTCTGTCTTATCGCTAGTAATACATAAATCAATTTAACATAAATAAATTTGATCGAAACTTAAACAAAACAACAAGGATGACTTATTTTTTTTAATCAAACCAAGGACTTCAACTTTTAACTAAGGACTTGCTTAAACAAGCCGCAACTTTTAAACTTATTTGAATCATTGTGTCTGACTCTAATATTAATAGGACCAACAACTTGTTATATTGAAGTTGTGTTTAATTAACTTTATATTGCACATCTGGTCCTCGTTGTTACATTTTAAGGGTTTAGCTGTGAGAATATTTTATGTGGAATTACAAATTCAAACATTAATGTATATGTTTATGTTTCCCTCTAAATATTTTTGATATTTCCCTCTATGAATATGTTAGGGCAAGGCCTTCTAGGCGTTATCCAGACTGTTTCAGAATTTGAAACTGATGCATCATGGAAAATAAAGGACGTGAAAGCTTTGCATATCATTCAACTATCATGTGGACGAGAGATTGCTGATGAGATTAGTCATTTTAAAACAGCCAAAAGCGCCTGGAATCATTTAGGCTCATTATATGGCAAAGATTTAACAGCCGGTGCAGATCATGAACAAGGTACCATTAACTGATTTAAAAGTTCCATAAACTGATTTTTATGACAATTAATGTGCCTCATCAAACTCTTGAATGTTAATGGCAGATGTGGATGATAGCCACTCTCCAAAGAATAAAGAATTGTTCTGGAAGGTGATGAGAGGTACGCCTATTGGAAATATCGGTATGGACTCGAATGTATATATGACATCGGCTTCCAGGTCTTCGCTTCTTCATGCTGCAGTAATTGCTGGAAATGTGGAAAATGTGGAATTGTTGGTGAAAGTAGGGAAAGACACGTTAATATGCATGCAAGACGAACACGGTGACACAGCTCTTGCGCTTGTTGCACGTTATACTGGAAATACCGAAATAGCAAAGTGCATGGTAGAGGAAATAAAGGGACTCCCAGAAACGTTGCTAGAAAAGGAGAACAATGACAATGTAATCCCTATTCTTCTAGCTGCTGCTAATGGACACAAAGAATTGACCACCTATCTTTACTCCAAAACCCCCACCAAATCCAAAGTGTTCGACAAATCCAATTCACAGAATCGAGTTTTGCTTCTTTCACTATGTATCACTGCTGAAATATTTGGTAAGAAAATTTATAGTTTTTGTTTGTTGTTAAATTTTTTATTTTATTTTAATGTAATACTTTTTTTATTCAAATGTGTGGTTTGATCCATGATCAGATGTGGCTTTGAGATTACTCAGGCGTTTCAAAGATCTGCCTGAGCAATCATTATCAATATATAATTTCTCCGTCCCTACGTTGTTAAGGGAATCATTATCACTACCTAGCAAAATCTCAGAATCAGATCCTCTCCAGCAATCCTTACCTGATAAATTCTCGGCCTTAGTTGCATTGGCAAAAATGCCTACATCTTTTCCAAGTGGCACCCAATTGGGATTTCTAGGCCAAATTATTTATGAAAGTAAGTTTTCACACTATTCTTTTTTTATTGGTACCAGGCAAACATTCCAATACTCTGAAATACATATTGTAAAAGAAAAATAGTTTAAATAAAGTTAGATGGTTAATACATATAACGACAACAACAAAACCCAATAGATACTATTCAGATTAAACTCAAGATAATATGTCATGTAATTGATTTTGATTGTGTTGATAAACTTATCTCTTTGAGTTAAAACATGTGGGTTCAATGTGGCTGCAATAGAAAAATACATAAAACCTAAGTTAGTAATATGATAATAACGAATTGATGATGGAGATTATGAAAGATGATAGAATAATAAGATACATGTCAAATATTGTAAAGCATGAGCGTCCTCAACACTGAGATTTTCCATGAGCATGTGAGATTGAGGTTCAACTGGTATGCAATGACGTCACATAAAAATAATGAGCATCAATTAATATAAAAAGAAGCGGGTTGTGAGTTTTTACGGTTTTGGTGATGTGGTGCAATTACTGAGATTATGGGTGGGAATTAGGCTTAAGAGGGGATTAGGGTTGAGCATAATTTAAGAGACATTTATAATAATAGAATAGATAGATAAATATAATATGATACAATGGTTATGGTAATTTGTACATTTATGGCGTATTAATAAGTTATTTAAAAAGGACGTGGAACAAATATAAGAAAAAATTAAATGATGAATCGTTCGGGAGAAGAAGTTGGATTTTTGGATGAAACAATTAAAATCTCACAACCAAACTACCGATTACTAGGGGGACTCTTTTTCCTGGGATCTAAAGGATTAACAAAAATAAAAAAAAGATAAGTAAAGGGTTATGTTAGTATATCCATGACGATGATCATTTAAAATTTACCAGTTCTTTATCAACATTAGTGATTAAATTTATATTATTCCTTCCGGTTCTTTTTATAAAAGATAAATGTGTCAACAAAAATTGATGTATTTAGTTTATAATATTGACTAAATACATCAATTTTCGTTGATCCGTTTTTCTCTTATAAAAAAGTACCGAAAGGAGAGTATATGTAGATATTTATCCATATAATGATAGTTAAATATTAGATCATAAAAGAAAGAAAGAAACAATGTAATAAAATATTTGAATAGAGGAGGATTGCTATATGCTGCGAAGGAAATGATGCACGACCTGGATACGACAAAACGTGGGCAATGCTTTCACACTTTTAAAATAAATAAAGTAAAACACCACTTAAAGCAAAACTAAATCCAAGGGCTGGTGTGCATTCGTCGTTATTAGTCAACTCCTCTCTCGCACCATTTATCTTTCTCCATTTGAATAAACCTCTTTTAAAAAGAAAATATTTGAACATAATTTTCATTGCAGATTTGAGCGTGGAAAGGAGTTTCAACATTGAGTATGATATTGCCGAAATTGGGAACTTCGTCCGAAAGGTAACATGTGTTGCTGATGCTGCTAATGAAAGACCACAAAAGATTTCTTCCGCAGTCCAATGTGGCATAGGCAGAAAGGTTTTGAATTTCGTTGTTCGAATAATGCTTTCCGGTACGCTATGTTTTTTCTCTTTAACTATTTCAGTCTTCGAACATCACATATTAATAATCAAGACCTATGCCTTTTAACCGATACTTTTATGACACAAGTTAGCAAAACCTGTTTTTTATTACCCACATGAATATATATAAATGGCTCAAAGCTTTTATTTAGGTTGGCGCTTCTGCAAACTGTTGATATCGATATTTCTCTTTTGGCCAGTAAAGCTTTTGGGTATGTCGTGTACTGTCTCAGTTTGCAATATCATGCTTTAATCATTTTTTATTTCTTCTCTGATATTTTTTCTTTTTATTATTTTTTTAAGGATTTCTTTTTACTATTTCTACACTGATTTTGTTGATTTGTTCAGGTCAACTACTTTGTAAGATATTCCGTGGAGTTGCATATTTGTTGGTTCAAGTTTTCCATTACCTAGATAGTAATGGTAAGTAAATTCAAATGCAAAAGTTTGATTCATTTCAATAATCATCCAGTAAAAAAAAACTCATTAATTATTCCCTCACATTTCAAATATGTGATGAATTAGGGGAGCGGTTAAAAAGTACGACTGATTTTTATACGAAAATTCACGAAGTGCAAATTGATATAAAAAAAATGTGTAATTTCCCCTCGTTTCTTTGAAAAAAGAACTAGATTAAACTAAAAAATAAAAAATAGAACAAACTTGACATAAAAGCTTTCTCATGCCAATAAGAGTGAAGTCGGCAAAATTTGGAACAAGGGAGTTTAAAATCAATTTCATTATTTTTTGCAAGCTGCTTTGAAAAATCAATTCAAAAAACGTGTTAGCATGGAAGATGCCGACTCATAAATCACATCATGACGAATTTAAAGTTCTCTTTTACTCTCAAATTAAAAAGTTGTTTAAATAATGGAAAAAAATTACTTTGATTCATGAAATTTTGTATATACGATCAAATATTGGTTTCACTAATTAGAAAACTTCAATGTTCCAACCTGCAAAACTGTCTGCAACTCATGCGGAAACCTATATCAGTAGGAAAACATGTAACATCAGCTATCACACGAGTCAATTCACTTTGCACCAATACTAATAACAATTGATATACTAATTTGCAATAGACACGAATACAATTATACACTATAAACCATACACCAGTCAACAAATCGATCGAACCGCAAAATATTGAACCATATTTAAAACATAATCCCTCCTACAATATAATTACTAATATAGTTGGAACGTCAACAGATCATAGTGATGTCTTAGCATTTAAAATATTGGTTAAAAAAATCATTATGATTGATAGTTAAGAAAAAATCTTGATATTGATGTTGCATGTAAAGCTTAATTCGAATTGATTTTTTTTAACAACTCCAAGACTGATTAGGTCCCTTCAATTATGTCAGGAATCAGGAAGATATATGATCAGAAATACACACATTATGAAGTTATAGGAATATTGAGTTACTTCTGCCGAAGTGTTGGAAAATTTAATAGTTCAAAGCTCAAAGAGGCTTCCGCGTATGAAGCAATGTTGCATGCATCCCAACACGGAATAATCGAGTTCATAAATGCCATGAGGGATGCTAATCCTAATTTTCTTTCAGCAGTTGATAGCTGCCAGAGAGGCATATTTTCGTATGCAATCTTGCATCGCAAACAAAATGTGTTTCAACTCATACATTCTGTCAATGGACGGAAGGAGATACTTAGACATCGTATAGACTCGTTTGGCAATAATTTGTTGCACCTGGCAGCACATTTAGGTCCTTCATCTGATCGTCATAGCAGATCCGGTGCTGCTCTGCAAATGCAAAGAGAGATTCAATGGTTTAAGGTCACCCATCTAAATTAATTGTCTTAAATATACAATTGATTGAAGATACATATTCCATATTTTAATTCAATATTAGAAGTGCAAAATAATCAACGTATACTAGCTTTGTTGGCCATTTTACAAAACATGGTATAGGAAAATCCTTTTAGATTACATGTTATAAATACATATTAAGCATTTCCTAATAAATGTTGTTGAAACACCATATGTTCAGTACTCTAAAATTACACTCCAACAGTATTAGGTACGAAACACCCCTTCTGTTACGAAATATAAGTAAAAATGAGTTAGTTTTTTTGGACCAAATACATCAAACTTTCGTTAACCAACTTTTGCTTATATTTCGTTACAAATGTTGTTACAAAGCCTCCAAATGAACCAAGATTCATGTGTTTCAACATTGTGATTTTAAAGAAGCTTCAAAACCACATAATTCCACCAATCTATTTAATTTACAGTATATGCATGTCTCTTGCTTCTTTATAAGTTGTTTATGATACCACTGCAGGCGGTTGAGAAAGTTTTACATCCCAAGTTTAAGGAAGCCGAAAATGATGATGGTAAGAAGCCTTATGAGATATTCATTGAAAGCCATGAGGAGCTAGTGAAAGATGGAGAGAAATGGGCAAAAGACACAGCAACATCTTATACAATTGTAGGTACTCTCATCACCACCATCATGTTTGCTGCAGCCTTTACAGTGCCAGGTGGAAACGACGACAAAACTGGTTTGCCCATCTTCTTACATGACAACATATTCACTGCATTTCTCATGGCAGACGCAGTATCTCTCTTCGCATCTGCGACTTCCGTCTTGATTTTCATTGGTATACTCACATCGCGTTATGCTGAGACAGACTTTCTCAAGAGCTTACCTTGAAAGTTATTGTTTGGTCTTTTGCTCCTTTTTCTCTCTGTTTGTTCTATGATAGTTGCATTTAGCGCTGCCATTATCGACATGATCTTAAAGGGATATGAAACCCATAAGTGGTTCATTGTAGTACCAATCATGGCACTTGGGAGTATTCCGATTATCGTTCTTGTAATATCACAGGTGAGCTTCATGTATGAAATCTTCCGCTCAACATGGAAAAATCCCATCCGTTCAATAAACAAATGACGACAGTAATATGTATGCTTCAAACGATTCATACTTGCCTTGCCTCAATAAAAGGTATATATTCCTTATGAGAAAGATAGAGATTTTATCAAATAATCTTGTACTTATCATTGTTAATGCACCTATTTCCTTCCATTGTTTCATGCATCTGTAATTTTTCACTAAAAGAATGAATAATCTTGTACTTCTCATTGCAAAAGCCCTTGTTTTTTTTTTCTTTAGCAAAAAAATATTGCATACTATGCTGGTTTAACACCCCAACCAAAGTCCAAGAGATATGGGGGAATATGTGGCATCCATTTCAGCAAATTAGAAAAGTCACATTTGATACGAGTCTCTCTTTGGATAAAAGTATCTTCATATTTTGCAACTATAAATAGTAGTAAACCACATCGTACAAAGTATAATGATTTAAGATTTCCTTTTAACCAAATAGAATTTGATCATAACTTAGAAATATTCTGAATCTTGAAACTTCCTGCTCTCTTGTTCTTGATTCAAGGTGTAAATTTCCAAATGTAAAATAGAGCAAAATCAAGCAAAACACGTGTCAAAACAAAGAGAAAATATTTAAATAAATTGGAAATGTATTTTAAACTCATATAGTTTAGGAAAAATGTTACCGATTTCCATGTGCAAGGAGGACTTCATGGACCCAACTTGGAGTACCACATCGGAAGTCATGAACCCGGTGTGAGGATTTATATAGAATTGGAAAATACTCTTGTTTTAGCCATGCAGAGTTAG
Protein sequence

>MS.gene98479.t1

MMKSTGVASDGIVHQVLTKDNYERWKIVMQNYLQGQGLWDVVIQNAESRTDQASKKMKDGQQALHFFQLSSCKRDFLETVKKSGWNNFTNQNDPDSRATPDLEQGQGLLGVIQTVSEFETDASWKIKDVKALHIIQLSCGREIADEISHFKTAKSAWNHLGSLYGKDLTAGADHEQDVDDSHSPKNKELFWKVMRGTPIGNIGMDSNVYMTSASRSSLLHAAVIAGNVENVELLVKVGKDTLICMQDEHGDTALALVARYTGNTEIAKCMVEEIKGLPETLLEKENNDNVIPILLAAANGHKELTTYLYSKTPTKSKVFDKSNSQNRVLLLSLCITAEIFDVALRLLRRFKDLPEQSLSIYNFSVPTLLRESLSLPSKISESDPLQQSLPDKFSALVALAKMPTSFPSGTQLGFLGQIIYENLSVERSFNIEYDIAEIGNFVRKVTCVADAANERPQKISSAVQCGIGRKVLNFVVRIMLSGQLLCKIFRGVAYLLVQVFHYLDSNGIRKIYDQKYTHYEVIGILSYFCRSVGKFNSSKLKEASAYEAMLHASQHGIIEFINAMRDANPNFLSAVDSCQRGIFSYAILHRKQNVFQLIHSVNGRKEILRHRIDSFGNNLLHLAAHLGPSSDRHSRSGAALQMQREIQWFKAVEKVLHPKFKEAENDDGKKPYEIFIESHEELVKDGEKWAKDTATSYTIVGTLITTIMFAAAFTVPGGNDDKTGLPIFLHDNIFTAFLMADAVSLFASATSVLIFIVAFSAAIIDMILKGYETHKWFIVVPIMALGSIPIIVLVISQFRKNVTDFHVQGGLHGPNLEYHIGSHEPGVRIYIELENTLVLAMQS