AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene98660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98660.t1 MTR_0040s0140 96.875 256 8 0 1 256 1 256 0.0 521
MS.gene98660.t1 MTR_2g090255 79.365 252 51 1 6 256 1 252 1.13e-158 441
MS.gene98660.t1 MTR_5g076950 60.177 226 89 1 31 256 40 264 4.10e-98 288
MS.gene98660.t1 MTR_5g079950 51.304 230 112 0 27 256 28 257 5.24e-88 262
MS.gene98660.t1 MTR_7g061720 50.435 230 114 0 27 256 27 256 1.41e-87 261
MS.gene98660.t1 MTR_5g075320 48.996 249 125 1 8 256 5 251 6.02e-87 259
MS.gene98660.t1 MTR_2g097030 50.435 230 114 0 27 256 29 258 1.59e-86 258
MS.gene98660.t1 MTR_3g094060 50.000 246 121 1 11 256 10 253 4.84e-86 256
MS.gene98660.t1 MTR_3g064510 55.932 236 91 6 31 256 52 284 7.34e-86 257
MS.gene98660.t1 MTR_5g041700 50.000 244 120 1 13 256 6 247 2.06e-85 255
MS.gene98660.t1 MTR_3g435430 52.632 228 106 1 29 256 22 247 3.37e-85 254
MS.gene98660.t1 MTR_3g094040 52.889 225 104 1 32 256 31 253 5.05e-85 254
MS.gene98660.t1 MTR_4g081950 50.410 244 119 1 13 256 42 283 1.93e-84 254
MS.gene98660.t1 MTR_7g013140 49.794 243 117 2 12 254 11 248 2.71e-84 252
MS.gene98660.t1 MTR_3g466790 53.778 225 102 1 32 256 32 254 7.63e-84 251
MS.gene98660.t1 MTR_4g063975 48.438 256 124 2 9 256 1 256 1.01e-83 251
MS.gene98660.t1 MTR_3g466760 53.333 225 103 1 32 256 38 260 5.25e-83 249
MS.gene98660.t1 MTR_7g013150 48.347 242 120 2 13 254 14 250 1.06e-80 243
MS.gene98660.t1 MTR_7g011060 48.387 248 113 3 14 256 3 240 6.03e-80 241
MS.gene98660.t1 MTR_8g058990 50.667 225 109 1 29 253 27 249 5.37e-79 239
MS.gene98660.t1 MTR_8g464350 46.939 245 128 1 8 252 7 249 2.48e-77 234
MS.gene98660.t1 MTR_7g013120 51.316 228 108 2 29 255 26 251 2.77e-77 234
MS.gene98660.t1 MTR_4g124340 50.667 225 108 2 32 255 61 283 7.31e-77 234
MS.gene98660.t1 MTR_1g085460 49.356 233 114 3 25 254 23 254 4.59e-76 231
MS.gene98660.t1 MTR_7g111010 46.559 247 124 4 13 252 4 249 7.38e-75 228
MS.gene98660.t1 MTR_7g110990 49.554 224 109 3 32 252 12 234 2.58e-74 226
MS.gene98660.t1 MTR_6g012520 48.606 251 114 3 11 256 2 242 7.20e-73 223
MS.gene98660.t1 MTR_1g026020 42.188 256 140 5 3 254 1 252 3.01e-70 216
MS.gene98660.t1 MTR_5g011210 41.532 248 143 2 8 254 13 259 2.18e-67 209
MS.gene98660.t1 MTR_4g102450 43.983 241 133 2 13 252 17 256 3.44e-67 209
MS.gene98660.t1 MTR_0008s0120 51.852 108 52 0 146 253 88 195 4.48e-34 122
MS.gene98660.t1 MTR_0008s0120 39.326 89 54 0 1 89 1 89 8.59e-14 68.6
MS.gene98660.t1 MTR_6g012500 54.000 100 46 0 157 256 181 280 1.80e-33 122
MS.gene98660.t1 MTR_2g090270 31.429 245 140 9 23 254 26 255 1.07e-29 112
MS.gene98660.t1 MTR_2g090265 30.962 239 137 10 29 254 30 253 9.29e-28 107
MS.gene98660.t1 MTR_0122s0020 32.353 238 135 9 29 254 38 261 1.13e-27 107
MS.gene98660.t1 MTR_4g028640 31.933 238 131 10 29 252 39 259 9.04e-25 99.8
MS.gene98660.t1 MTR_0118s0070 29.130 230 135 11 37 251 53 269 7.40e-19 84.0
MS.gene98660.t1 MTR_0809s0010 29.600 250 142 13 18 252 35 265 2.27e-17 79.7
MS.gene98660.t1 MTR_0122s0020 32.597 181 97 8 29 198 38 204 3.23e-16 75.5
MS.gene98660.t1 MTR_5g013440 28.194 227 146 9 35 254 32 248 1.48e-13 68.9
MS.gene98660.t1 MTR_1g085365 51.786 56 27 0 201 256 4 59 2.82e-12 60.8
MS.gene98660.t1 MTR_4g075790 26.106 226 132 10 43 252 32 238 3.91e-12 64.7
MS.gene98660.t1 MTR_4g099370 25.664 226 151 8 34 252 30 245 3.94e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98660.t1 AT4G38210 65.306 245 81 2 14 256 12 254 2.52e-119 341
MS.gene98660.t1 AT3G03220 57.522 226 95 1 31 256 39 263 1.57e-93 276
MS.gene98660.t1 AT2G39700 48.413 252 130 0 5 256 4 255 1.03e-89 266
MS.gene98660.t1 AT3G55500 50.602 249 119 1 12 256 10 258 2.42e-89 265
MS.gene98660.t1 AT5G02260 47.600 250 126 1 12 256 7 256 3.01e-88 262
MS.gene98660.t1 AT2G37640 49.402 251 119 1 14 256 10 260 5.54e-88 262
MS.gene98660.t1 AT5G05290 51.271 236 112 2 22 256 22 255 1.06e-85 256
MS.gene98660.t1 AT2G03090 49.609 256 125 3 1 256 1 252 2.97e-85 254
MS.gene98660.t1 AT2G40610 50.201 249 121 3 9 256 7 253 2.44e-84 252
MS.gene98660.t1 AT1G69530 50.202 247 119 2 12 256 5 249 5.28e-84 251
MS.gene98660.t1 AT1G69530 50.202 247 119 2 12 256 5 249 5.28e-84 251
MS.gene98660.t1 AT5G56320 48.560 243 123 1 14 256 11 251 6.15e-84 251
MS.gene98660.t1 AT5G56320 48.560 243 123 1 14 256 11 251 6.15e-84 251
MS.gene98660.t1 AT2G37640 53.810 210 97 0 47 256 26 235 8.08e-84 250
MS.gene98660.t1 AT5G56320 48.560 243 123 1 14 256 11 251 2.63e-83 251
MS.gene98660.t1 AT1G69530 50.413 242 116 2 12 251 5 244 3.67e-82 248
MS.gene98660.t1 AT2G28950 46.667 255 135 1 3 256 1 255 1.65e-81 245
MS.gene98660.t1 AT5G39280 51.327 226 106 2 29 254 51 272 8.65e-79 239
MS.gene98660.t1 AT5G39300 50.442 226 108 2 29 254 52 273 9.47e-78 236
MS.gene98660.t1 AT5G39270 50.885 226 107 2 29 254 55 276 5.27e-77 234
MS.gene98660.t1 AT5G39290 50.442 226 108 2 29 254 55 276 2.87e-76 233
MS.gene98660.t1 AT1G26770 48.207 251 127 2 6 256 1 248 4.89e-76 231
MS.gene98660.t1 AT1G26770 48.016 252 128 2 5 256 10 258 5.52e-76 231
MS.gene98660.t1 AT3G29030 46.768 263 118 5 6 256 1 253 1.25e-75 230
MS.gene98660.t1 AT1G62980 43.621 243 135 2 13 254 12 253 9.63e-74 226
MS.gene98660.t1 AT5G39260 43.922 255 128 4 6 254 14 259 3.26e-70 217
MS.gene98660.t1 AT4G01630 46.875 224 115 3 32 252 28 250 4.72e-70 216
MS.gene98660.t1 AT5G39310 48.661 224 110 3 32 254 90 309 1.39e-69 217
MS.gene98660.t1 AT1G20190 43.966 232 125 3 24 252 18 247 2.85e-69 214
MS.gene98660.t1 AT1G69530 44.130 247 104 3 12 256 5 219 1.57e-68 211
MS.gene98660.t1 AT1G69530 49.510 204 99 2 12 213 5 206 2.54e-67 209
MS.gene98660.t1 AT1G12560 43.443 244 133 4 12 252 15 256 4.20e-67 209
MS.gene98660.t1 AT3G15370 44.358 257 131 6 6 256 1 251 3.83e-66 206
MS.gene98660.t1 AT3G15370 40.767 287 128 8 6 256 1 281 2.19e-61 195
MS.gene98660.t1 AT1G20190 46.032 189 97 3 67 252 2 188 5.62e-59 186
MS.gene98660.t1 AT4G28250 32.365 241 132 11 29 254 33 257 2.80e-24 98.2
MS.gene98660.t1 AT2G20750 32.669 251 141 11 18 254 28 264 1.54e-23 96.7
MS.gene98660.t1 AT1G65680 29.839 248 143 9 22 252 35 268 2.08e-23 96.3
MS.gene98660.t1 AT4G28250 32.083 240 128 11 29 254 33 251 2.83e-22 92.8
MS.gene98660.t1 AT2G45110 30.172 232 135 11 35 252 36 254 7.84e-18 80.9
MS.gene98660.t1 AT1G65681 28.636 220 132 9 45 252 11 217 3.10e-17 78.6
MS.gene98660.t1 AT3G60570 25.483 259 159 10 15 254 11 254 1.22e-14 72.0
MS.gene98660.t1 AT2G20750 32.804 189 103 8 18 195 28 203 2.98e-14 70.1
MS.gene98660.t1 AT3G60570 25.792 221 136 9 47 254 1 206 2.53e-11 62.0
MS.gene98660.t1 AT4G17030 25.969 258 151 11 13 252 9 244 2.61e-11 62.4

Find 61 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTTCTGTCCTCCTAATTT+TGG 0.230059 4.4:-59266979 MS.gene98660:CDS
GCACCTCCAAACTGGCAATT+TGG 0.255098 4.4:-59266181 MS.gene98660:CDS
TAGAGATCCTTCTGTGTCTT+TGG 0.259434 4.4:+59267379 None:intergenic
TTTGGTCTCTCAGTTGATTA+TGG 0.289936 4.4:-59266961 MS.gene98660:CDS
GGATACCAATGGCAAGGAAT+TGG 0.318465 4.4:-59266300 MS.gene98660:CDS
GTTTGTCCAAATTGCCAGTT+TGG 0.334344 4.4:+59266175 None:intergenic
CTAAATGCTGTTATCTAAGA+TGG 0.344241 4.4:-59267501 None:intergenic
TCAACTGAGAGACCAAAATT+AGG 0.348503 4.4:+59266967 None:intergenic
GATACCAATGGCAAGGAATT+GGG 0.350848 4.4:-59266299 MS.gene98660:CDS
ACATCAGCCTTTGTCCTTTG+AGG 0.354377 4.4:-59266245 MS.gene98660:CDS
GACAACTCAAAATGTTCTCT+TGG 0.372662 4.4:+59266922 None:intergenic
GCAGAAGGAGCTTGTGGTTA+TGG 0.372980 4.4:-59267159 MS.gene98660:intron
ACAGAAGGATCTCTAATTAC+AGG 0.385780 4.4:-59267371 MS.gene98660:intron
TTATTCCCGTTCAATATAGA+AGG 0.389617 4.4:-59266858 MS.gene98660:intron
CAATTTGGACAAACATTTGA+AGG 0.398107 4.4:-59266166 MS.gene98660:CDS
TTGCTTACAAAGATCAAGAA+TGG 0.419745 4.4:-59267424 MS.gene98660:CDS
CAAGGAATTGGGGAATGAAT+TGG 0.429950 4.4:-59266288 MS.gene98660:CDS
ATCTACCTTCTATATTGAAC+GGG 0.431748 4.4:+59266853 None:intergenic
CAAGCATACACCACAAGATA+TGG 0.436575 4.4:+59267031 None:intergenic
ATAACTAATGTGGGGTTAGA+TGG 0.442300 4.4:-59266364 MS.gene98660:CDS
ACAATGTAGCACCTCCAAAC+TGG 0.451802 4.4:-59266189 MS.gene98660:CDS
AAAATTAGGAGGACAGAAAT+CGG 0.477715 4.4:+59266981 None:intergenic
ATTCCCCAATTCCTTGCCAT+TGG 0.501334 4.4:+59266295 None:intergenic
AAAGTGAAGGGATCTAGAAC+AGG 0.502893 4.4:-59266325 MS.gene98660:CDS
TGCATTTGCTGAAATTGCTA+AGG 0.506123 4.4:-59266893 MS.gene98660:CDS
TCTCAGTTGATTATGGTGGT+TGG 0.506701 4.4:-59266954 MS.gene98660:CDS
CAAGTTCTGATAACTAATGT+GGG 0.508543 4.4:-59266373 MS.gene98660:CDS
TCAAGTTCTGATAACTAATG+TGG 0.509065 4.4:-59266374 MS.gene98660:CDS
TAAATGCTGTTATCTAAGAT+GGG 0.517060 4.4:-59267500 MS.gene98660:CDS
AGGACAAAGGCTGATGTTGA+AGG 0.517191 4.4:+59266251 None:intergenic
GATCTTCACAAAGCTAGCTA+TGG 0.521811 4.4:-59267135 MS.gene98660:CDS
CATATCTTGTGGTGTATGCT+TGG 0.525681 4.4:-59267030 MS.gene98660:CDS
GTCTTTGGCATAAGTTGCAG+TGG 0.528308 4.4:+59267394 None:intergenic
TATCTACCTTCTATATTGAA+CGG 0.536318 4.4:+59266852 None:intergenic
TGTCCAAATTGCCAGTTTGG+AGG 0.539604 4.4:+59266178 None:intergenic
GTGAAGTGTGAAAGAAGTGG+AGG 0.540916 4.4:-59266433 MS.gene98660:CDS
GCATTTGCTGAAATTGCTAA+GGG 0.541056 4.4:-59266892 MS.gene98660:CDS
TTTGAGGTAACTAGCAGCAC+TGG 0.546125 4.4:-59266229 MS.gene98660:CDS
TTCAACAGAGGGAGTACATG+TGG 0.560849 4.4:-59267081 MS.gene98660:CDS
AGCTATGGAAAACATAGTGT+TGG 0.560850 4.4:-59267120 MS.gene98660:CDS
GGGTTGAAATTCACAATGAG+TGG 0.572612 4.4:-59266412 MS.gene98660:CDS
GGTCTCTCAGTTGATTATGG+TGG 0.580447 4.4:-59266958 MS.gene98660:CDS
ATACCAATGGCAAGGAATTG+GGG 0.587228 4.4:-59266298 MS.gene98660:CDS
TCAACAGAGGGAGTACATGT+GGG 0.614475 4.4:-59267080 MS.gene98660:CDS
TAAAATCAAGTAGAGAAGTG+TGG 0.616273 4.4:+59267466 None:intergenic
TGAAGTGTGAAAGAAGTGGA+GGG 0.622177 4.4:-59266432 MS.gene98660:CDS
TGAAGGGATCTAGAACAGGA+TGG 0.625878 4.4:-59266321 MS.gene98660:CDS
ACTGAGAGACCAAAATTAGG+AGG 0.626412 4.4:+59266970 None:intergenic
CAGGATGGATACCAATGGCA+AGG 0.630186 4.4:-59266306 MS.gene98660:CDS
CTAGTTACCTCAAAGGACAA+AGG 0.637645 4.4:+59266238 None:intergenic
TAGAACAGGATGGATACCAA+TGG 0.662207 4.4:-59266311 MS.gene98660:CDS
CAACAGAGGGAGTACATGTG+GGG 0.668401 4.4:-59267079 MS.gene98660:CDS
ACTTATGCCAAAGACACAGA+AGG 0.668875 4.4:-59267386 MS.gene98660:CDS
GTGGCAGTAACAATAACAGA+AGG 0.670978 4.4:+59267003 None:intergenic
AGTGCTGCTAGTTACCTCAA+AGG 0.674064 4.4:+59266231 None:intergenic
CATTTGCTGAAATTGCTAAG+GGG 0.677827 4.4:-59266891 MS.gene98660:CDS
AAGTTCTGATAACTAATGTG+GGG 0.688292 4.4:-59266372 MS.gene98660:CDS
TGTGATGCAGAAGGAGCTTG+TGG 0.691735 4.4:-59267165 MS.gene98660:intron
AGAGTGAAGTGTGAAAGAAG+TGG 0.695816 4.4:-59266436 MS.gene98660:CDS
ATTAGGAGGACAGAAATCGG+TGG 0.724565 4.4:+59266984 None:intergenic
GATGTGTTGACCATATCTTG+TGG 0.783191 4.4:-59267041 MS.gene98660:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACTTAATTAATCTCTTATT+TGG - chr4.4:59266861-59266880 MS.gene98660:CDS 15.0%
!! GTTTATATAAAAATAGTTGA+AGG + chr4.4:59266946-59266965 None:intergenic 15.0%
!!! AAATCATGTACATTTTTTTT+TGG + chr4.4:59266456-59266475 None:intergenic 15.0%
!!! AATCATGTACATTTTTTTTT+GGG + chr4.4:59266455-59266474 None:intergenic 15.0%
!!! ACTATTTTTATATAAACGTT+TGG - chr4.4:59266949-59266968 MS.gene98660:CDS 15.0%
!! AAATGAAACTAAAAAACACA+AGG + chr4.4:59267013-59267032 None:intergenic 20.0%
!! ATAACATAGCTAATAAAAAG+AGG + chr4.4:59267095-59267114 None:intergenic 20.0%
!! GTTAATGATGTTTATATAAG+CGG - chr4.4:59266883-59266902 MS.gene98660:CDS 20.0%
!!! TGTGTTTTTTAGTTTCATTT+TGG - chr4.4:59267013-59267032 MS.gene98660:CDS 20.0%
! AAAAAGAGGCAAAAAAGATT+AGG + chr4.4:59267081-59267100 None:intergenic 25.0%
! GCACAAACAAAACAAAAATT+TGG + chr4.4:59267183-59267202 None:intergenic 25.0%
! TATCTACCTTCTATATTGAA+CGG + chr4.4:59266801-59266820 None:intergenic 25.0%
!!! AAGTGTTTTTGAGATGTTTT+TGG - chr4.4:59266832-59266851 MS.gene98660:intron 25.0%
AAAATTAGGAGGACAGAAAT+CGG + chr4.4:59266672-59266691 None:intergenic 30.0%
ATCTACCTTCTATATTGAAC+GGG + chr4.4:59266800-59266819 None:intergenic 30.0%
CAATTTGGACAAACATTTGA+AGG - chr4.4:59267484-59267503 MS.gene98660:CDS 30.0%
CATAATATATGCAAGATCCA+GGG - chr4.4:59266411-59266430 MS.gene98660:CDS 30.0%
TAAAATCAAGTAGAGAAGTG+TGG + chr4.4:59266187-59266206 None:intergenic 30.0%
TTATTCCCGTTCAATATAGA+AGG - chr4.4:59266792-59266811 MS.gene98660:intron 30.0%
TTGCTTACAAAGATCAAGAA+TGG - chr4.4:59266226-59266245 MS.gene98660:CDS 30.0%
! AAGTTCTGATAACTAATGTG+GGG - chr4.4:59267278-59267297 MS.gene98660:intron 30.0%
! CAAGTTCTGATAACTAATGT+GGG - chr4.4:59267277-59267296 MS.gene98660:intron 30.0%
! TCAAGTTCTGATAACTAATG+TGG - chr4.4:59267276-59267295 MS.gene98660:intron 30.0%
!!! CATGTACATTTTTTTTTGGG+TGG + chr4.4:59266452-59266471 None:intergenic 30.0%
ACAGAAGGATCTCTAATTAC+AGG - chr4.4:59266279-59266298 MS.gene98660:CDS 35.0%
AGCTATGGAAAACATAGTGT+TGG - chr4.4:59266530-59266549 MS.gene98660:intron 35.0%
ATAACTAATGTGGGGTTAGA+TGG - chr4.4:59267286-59267305 MS.gene98660:intron 35.0%
GACAACTCAAAATGTTCTCT+TGG + chr4.4:59266731-59266750 None:intergenic 35.0%
GCATAATATATGCAAGATCC+AGG - chr4.4:59266410-59266429 MS.gene98660:CDS 35.0%
TCAACTGAGAGACCAAAATT+AGG + chr4.4:59266686-59266705 None:intergenic 35.0%
TTTGGTCTCTCAGTTGATTA+TGG - chr4.4:59266689-59266708 MS.gene98660:intron 35.0%
! AGCACAATTTTGTTCAACAG+AGG - chr4.4:59266557-59266576 MS.gene98660:intron 35.0%
! ATGTTGCTGTTATGAACCAA+AGG + chr4.4:59266321-59266340 None:intergenic 35.0%
! CATTTGCTGAAATTGCTAAG+GGG - chr4.4:59266759-59266778 MS.gene98660:intron 35.0%
! GATTTCTGTCCTCCTAATTT+TGG - chr4.4:59266671-59266690 MS.gene98660:intron 35.0%
! GCACAATTTTGTTCAACAGA+GGG - chr4.4:59266558-59266577 MS.gene98660:intron 35.0%
! GCATTTGCTGAAATTGCTAA+GGG - chr4.4:59266758-59266777 MS.gene98660:intron 35.0%
! GTTTGGCTCAATTACACATT+TGG - chr4.4:59266966-59266985 MS.gene98660:CDS 35.0%
! TGCATTTGCTGAAATTGCTA+AGG - chr4.4:59266757-59266776 MS.gene98660:intron 35.0%
!! AGTTTTTGCTGTGAAAGTGA+AGG - chr4.4:59267312-59267331 MS.gene98660:intron 35.0%
!! GTTTTTGCTGTGAAAGTGAA+GGG - chr4.4:59267313-59267332 MS.gene98660:intron 35.0%
!!! AGAACATTTTGAGTTGTCAC+TGG - chr4.4:59266733-59266752 MS.gene98660:intron 35.0%
!!! ATTTTTTTTTGGGTGGTGGT+CGG + chr4.4:59266445-59266464 None:intergenic 35.0%
!!! GTACATTTTTTTTTGGGTGG+TGG + chr4.4:59266449-59266468 None:intergenic 35.0%
ACTGAGAGACCAAAATTAGG+AGG + chr4.4:59266683-59266702 None:intergenic 40.0%
ACTTATGCCAAAGACACAGA+AGG - chr4.4:59266264-59266283 MS.gene98660:CDS 40.0%
AGAGTGAAGTGTGAAAGAAG+TGG - chr4.4:59267214-59267233 MS.gene98660:intron 40.0%
ATACCAATGGCAAGGAATTG+GGG - chr4.4:59267352-59267371 MS.gene98660:intron 40.0%
CAAGCATACACCACAAGATA+TGG + chr4.4:59266622-59266641 None:intergenic 40.0%
CAAGGAATTGGGGAATGAAT+TGG - chr4.4:59267362-59267381 MS.gene98660:intron 40.0%
CATATCTTGTGGTGTATGCT+TGG - chr4.4:59266620-59266639 MS.gene98660:intron 40.0%
CTAGTTACCTCAAAGGACAA+AGG + chr4.4:59267415-59267434 None:intergenic 40.0%
GATACCAATGGCAAGGAATT+GGG - chr4.4:59267351-59267370 MS.gene98660:intron 40.0%
GATCTTCACAAAGCTAGCTA+TGG - chr4.4:59266515-59266534 MS.gene98660:intron 40.0%
GGGTTGAAATTCACAATGAG+TGG - chr4.4:59267238-59267257 MS.gene98660:intron 40.0%
GTGGCAGTAACAATAACAGA+AGG + chr4.4:59266650-59266669 None:intergenic 40.0%
GTTTGTCCAAATTGCCAGTT+TGG + chr4.4:59267478-59267497 None:intergenic 40.0%
TAGAACAGGATGGATACCAA+TGG - chr4.4:59267339-59267358 MS.gene98660:intron 40.0%
TAGAGATCCTTCTGTGTCTT+TGG + chr4.4:59266274-59266293 None:intergenic 40.0%
TCTCAGTTGATTATGGTGGT+TGG - chr4.4:59266696-59266715 MS.gene98660:intron 40.0%
TGAAGTGTGAAAGAAGTGGA+GGG - chr4.4:59267218-59267237 MS.gene98660:intron 40.0%
! AAAGTGAAGGGATCTAGAAC+AGG - chr4.4:59267325-59267344 MS.gene98660:intron 40.0%
! GATGTGTTGACCATATCTTG+TGG - chr4.4:59266609-59266628 MS.gene98660:intron 40.0%
! TACTTTTCTTGCTCCTCCTT+TGG - chr4.4:59266302-59266321 MS.gene98660:CDS 40.0%
! TTGCTGTTATGAACCAAAGG+AGG + chr4.4:59266318-59266337 None:intergenic 40.0%
!!! TTTTTTTTTGGGTGGTGGTC+GGG + chr4.4:59266444-59266463 None:intergenic 40.0%
ACAATGTAGCACCTCCAAAC+TGG - chr4.4:59267461-59267480 MS.gene98660:CDS 45.0%
ACATCAGCCTTTGTCCTTTG+AGG - chr4.4:59267405-59267424 MS.gene98660:CDS 45.0%
AGGACAAAGGCTGATGTTGA+AGG + chr4.4:59267402-59267421 None:intergenic 45.0%
ATTAGGAGGACAGAAATCGG+TGG + chr4.4:59266669-59266688 None:intergenic 45.0%
ATTCCCCAATTCCTTGCCAT+TGG + chr4.4:59267358-59267377 None:intergenic 45.0%
GGATACCAATGGCAAGGAAT+TGG - chr4.4:59267350-59267369 MS.gene98660:intron 45.0%
GGTCTCTCAGTTGATTATGG+TGG - chr4.4:59266692-59266711 MS.gene98660:intron 45.0%
GTCTTTGGCATAAGTTGCAG+TGG + chr4.4:59266259-59266278 None:intergenic 45.0%
GTGAAGTGTGAAAGAAGTGG+AGG - chr4.4:59267217-59267236 MS.gene98660:intron 45.0%
TGTCCAAATTGCCAGTTTGG+AGG + chr4.4:59267475-59267494 None:intergenic 45.0%
TTGTGTGCATGTGATGCAGA+AGG - chr4.4:59266476-59266495 MS.gene98660:intron 45.0%
! AGTGCTGCTAGTTACCTCAA+AGG + chr4.4:59267422-59267441 None:intergenic 45.0%
! TCAACAGAGGGAGTACATGT+GGG - chr4.4:59266570-59266589 MS.gene98660:intron 45.0%
! TGAAGGGATCTAGAACAGGA+TGG - chr4.4:59267329-59267348 MS.gene98660:intron 45.0%
! TTCAACAGAGGGAGTACATG+TGG - chr4.4:59266569-59266588 MS.gene98660:intron 45.0%
! TTTGAGGTAACTAGCAGCAC+TGG - chr4.4:59267421-59267440 MS.gene98660:CDS 45.0%
!!! TTTTTTTTGGGTGGTGGTCG+GGG + chr4.4:59266443-59266462 None:intergenic 45.0%
CAGGATGGATACCAATGGCA+AGG - chr4.4:59267344-59267363 MS.gene98660:intron 50.0%
GCACCTCCAAACTGGCAATT+TGG - chr4.4:59267469-59267488 MS.gene98660:CDS 50.0%
GCAGAAGGAGCTTGTGGTTA+TGG - chr4.4:59266491-59266510 MS.gene98660:intron 50.0%
TGTGATGCAGAAGGAGCTTG+TGG - chr4.4:59266485-59266504 MS.gene98660:intron 50.0%
! CAACAGAGGGAGTACATGTG+GGG - chr4.4:59266571-59266590 MS.gene98660:intron 50.0%
CCAACTGAGCTAAGCTCACG+AGG + chr4.4:59266380-59266399 None:intergenic 55.0%
CCTCGTGAGCTTAGCTCAGT+TGG - chr4.4:59266377-59266396 MS.gene98660:CDS 55.0%
GGTGGTCGGGGTTTGAACCC+TGG + chr4.4:59266431-59266450 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 59266153 59267519 59266153 ID=MS.gene98660
chr4.4 mRNA 59266153 59267519 59266153 ID=MS.gene98660.t1;Parent=MS.gene98660
chr4.4 exon 59267372 59267519 59267372 ID=MS.gene98660.t1.exon1;Parent=MS.gene98660.t1
chr4.4 CDS 59267372 59267519 59267372 ID=cds.MS.gene98660.t1;Parent=MS.gene98660.t1
chr4.4 exon 59266859 59267177 59266859 ID=MS.gene98660.t1.exon2;Parent=MS.gene98660.t1
chr4.4 CDS 59266859 59267177 59266859 ID=cds.MS.gene98660.t1;Parent=MS.gene98660.t1
chr4.4 exon 59266153 59266456 59266153 ID=MS.gene98660.t1.exon3;Parent=MS.gene98660.t1
chr4.4 CDS 59266153 59266456 59266153 ID=cds.MS.gene98660.t1;Parent=MS.gene98660.t1
Gene Sequence

>MS.gene98660

ATGCTGTTATCTAAGATGGGTGTTCTTCAGTCCACACTTCTCTACTTGATTTTATTACATTCATTCAACATTGTTGCTTACAAAGATCAAGAATGGAAGAAAGCCACTGCAACTTATGCCAAAGACACAGAAGGATCTCTAATTACAGGTACTTTTCTTGCTCCTCCTTTGGTTCATAACAGCAACATTAATAGTCTTGTTTTGTCAAATGTACATGATTTTGTCCTCGTGAGCTTAGCTCAGTTGGTATAGACAATGCATAATATATGCAAGATCCAGGGTTCAAACCCCGACCACCACCCAAAAAAAAATGTACATGATTTTTGTGTGCATGTGATGCAGAAGGAGCTTGTGGTTATGGAGATCTTCACAAAGCTAGCTATGGAAAACATAGTGTTGGATTAAGCACAATTTTGTTCAACAGAGGGAGTACATGTGGGGCTTGCTATGAGATCAGATGTGTTGACCATATCTTGTGGTGTATGCTTGGAAGTCCTTCTGTTATTGTTACTGCCACCGATTTCTGTCCTCCTAATTTTGGTCTCTCAGTTGATTATGGTGGTTGGTGTAACTTTCCAAGAGAACATTTTGAGTTGTCACTGGCTGCATTTGCTGAAATTGCTAAGGGGAAAGCTGATATTATTCCCGTTCAATATAGAAGGTAGATAGATTTTTTTATAAGTGTTTTTGAGATGTTTTTGGATAAACAACTTAATTAATCTCTTATTTGGTTAATGATGTTTATATAAGCGGTTATGTATAAACAATTTTTATAATCATAGAATACTGTCCTTCAACTATTTTTATATAAACGTTTGGCTCAATTACACATTTGGTTTTTAACTTTCATTTTAGTTCCTTGTGTTTTTTAGTTTCATTTTGGTTGTAATTTAAGAGACTAATCTTAAATTGTAATTTAATTTAACCTAATCTTTTTTGCCTCTTTTTATTAGCTATGTTATAGAGCTTATTAAAATAAGTCAGAAAATAATTTATAAACATGTTATAAGTTTTCTTATAAACTCTTCCAAATTTTTGTTTTGTTTGTGCAAATGTGATGCAGAGTGAAGTGTGAAAGAAGTGGAGGGTTGAAATTCACAATGAGTGGAAGTTCTCATTTCTATCAAGTTCTGATAACTAATGTGGGGTTAGATGGTGAAGTTTTTGCTGTGAAAGTGAAGGGATCTAGAACAGGATGGATACCAATGGCAAGGAATTGGGGAATGAATTGGCATTGCAATGTCAACCTTCAACATCAGCCTTTGTCCTTTGAGGTAACTAGCAGCACTGGAAAAACACTCACATCTTACAATGTAGCACCTCCAAACTGGCAATTTGGACAAACATTTGAAGGAAAACAGTTTTAG
Protein sequence

>MS.gene98660.t1

MLLSKMGVLQSTLLYLILLHSFNIVAYKDQEWKKATATYAKDTEGSLITEGACGYGDLHKASYGKHSVGLSTILFNRGSTCGACYEIRCVDHILWCMLGSPSVIVTATDFCPPNFGLSVDYGGWCNFPREHFELSLAAFAEIAKGKADIIPVQYRRVKCERSGGLKFTMSGSSHFYQVLITNVGLDGEVFAVKVKGSRTGWIPMARNWGMNWHCNVNLQHQPLSFEVTSSTGKTLTSYNVAPPNWQFGQTFEGKQF