Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98783.t1 | XP_003626285.1 | 67.9 | 168 | 13 | 2 | 1 | 138 | 274 | 430 | 2.40E-50 | 208.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98783.t1 | Q940P8 | 60.7 | 168 | 25 | 2 | 1 | 138 | 274 | 430 | 4.1e-46 | 185.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98783.t1 | G7KYN5 | 67.9 | 168 | 13 | 2 | 1 | 138 | 274 | 430 | 1.7e-50 | 208.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049825 | MS.gene98783 | 0.83731 | 5.61E-57 | -1.69E-46 |
| MS.gene049873 | MS.gene98783 | 0.853556 | 2.21E-61 | -1.69E-46 |
| MS.gene050355 | MS.gene98783 | 0.801641 | 8.18E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene98783 | 0.831059 | 2.07E-55 | -1.69E-46 |
| MS.gene052584 | MS.gene98783 | 0.809652 | 1.70E-50 | -1.69E-46 |
| MS.gene052611 | MS.gene98783 | 0.805093 | 1.58E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene98783 | 0.825029 | 5.86E-54 | -1.69E-46 |
| MS.gene054651 | MS.gene98783 | 0.811811 | 5.81E-51 | -1.69E-46 |
| MS.gene055512 | MS.gene98783 | 0.800757 | 1.24E-48 | -1.69E-46 |
| MS.gene056642 | MS.gene98783 | 0.801223 | 9.97E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene98783 | 0.828401 | 9.18E-55 | -1.69E-46 |
| MS.gene058783 | MS.gene98783 | 0.800711 | 1.27E-48 | -1.69E-46 |
| MS.gene05954 | MS.gene98783 | 0.835654 | 1.48E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98783.t1 | MTR_7g067470 | 67.857 | 168 | 13 | 2 | 1 | 138 | 274 | 430 | 1.12e-67 | 214 |
| MS.gene98783.t1 | MTR_7g113470 | 67.857 | 168 | 13 | 2 | 1 | 138 | 274 | 430 | 1.18e-67 | 214 |
| MS.gene98783.t1 | MTR_3g465910 | 72.727 | 55 | 12 | 1 | 87 | 138 | 453 | 507 | 5.00e-17 | 77.4 |
| MS.gene98783.t1 | MTR_5g087560 | 31.746 | 126 | 75 | 1 | 12 | 137 | 322 | 436 | 2.00e-12 | 63.9 |
| MS.gene98783.t1 | MTR_3g109925 | 37.113 | 97 | 49 | 3 | 34 | 129 | 292 | 377 | 4.07e-11 | 60.1 |
| MS.gene98783.t1 | MTR_3g109925 | 37.113 | 97 | 49 | 3 | 34 | 129 | 349 | 434 | 4.51e-11 | 60.1 |
| MS.gene98783.t1 | MTR_8g105970 | 31.967 | 122 | 67 | 3 | 34 | 149 | 206 | 317 | 8.41e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98783.t1 | AT5G20890 | 61.212 | 165 | 23 | 2 | 4 | 138 | 277 | 430 | 4.99e-57 | 186 |
| MS.gene98783.t1 | AT3G11830 | 31.452 | 124 | 74 | 1 | 14 | 137 | 324 | 436 | 4.46e-11 | 60.1 |
| MS.gene98783.t1 | AT3G18190 | 33.884 | 121 | 65 | 2 | 34 | 149 | 353 | 463 | 7.87e-11 | 59.7 |
Find 42 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTTGCCATTACCATTTC+AGG | 0.181073 | 7.4:-35918868 | None:intergenic |
| CTTATCGAAGATATTATTAT+TGG | 0.183032 | 7.4:+35918061 | MS.gene98783:CDS |
| GAGCGTCTGGCTCTGGTAAC+TGG | 0.301470 | 7.4:+35917980 | MS.gene98783:CDS |
| GGCAAAGGAAATTGATTCAT+TGG | 0.322602 | 7.4:+35918882 | MS.gene98783:CDS |
| GCATTATCAGCGATGGTTGT+TGG | 0.348817 | 7.4:-35918974 | None:intergenic |
| GAAAAGATGAACAAGATAAT+AGG | 0.380452 | 7.4:+35917938 | None:intergenic |
| CATTATCAGCGATGGTTGTT+GGG | 0.398556 | 7.4:-35918973 | None:intergenic |
| TGCCCAAGCTTAACAGACTC+TGG | 0.403665 | 7.4:-35918031 | None:intergenic |
| GGCACTTTCCCGAGCACTCT+TGG | 0.435170 | 7.4:+35918946 | MS.gene98783:CDS |
| TGGAGGCGGGTGGCCTGAAA+TGG | 0.444431 | 7.4:+35918855 | MS.gene98783:CDS |
| TTCTGGTGTTGCAATGTGGC+AGG | 0.456604 | 7.4:+35918105 | MS.gene98783:CDS |
| AATGACAGTAGGTTGTTACT+TGG | 0.472228 | 7.4:+35918835 | MS.gene98783:CDS |
| AACCCAGAGTCTGTTAAGCT+TGG | 0.477537 | 7.4:+35918028 | MS.gene98783:CDS |
| ACCCAGAGTCTGTTAAGCTT+GGG | 0.494078 | 7.4:+35918029 | MS.gene98783:CDS |
| GCATTTGAGCTGAACATGTA+TGG | 0.504914 | 7.4:+35918637 | MS.gene98783:CDS |
| TTGGGATAGCCAAGAGTGCT+CGG | 0.506902 | 7.4:-35918955 | None:intergenic |
| AGTAGGTTGTTACTTGGAGG+CGG | 0.510127 | 7.4:+35918841 | MS.gene98783:CDS |
| AAGTAACAACCTACTGTCAT+TGG | 0.511750 | 7.4:-35918833 | None:intergenic |
| GACAGTAGGTTGTTACTTGG+AGG | 0.514052 | 7.4:+35918838 | MS.gene98783:CDS |
| CGAAGATATTATTATTGGTG+AGG | 0.514475 | 7.4:+35918066 | MS.gene98783:CDS |
| AGGAAATTGATTCATTGGCT+AGG | 0.530474 | 7.4:+35918887 | MS.gene98783:CDS |
| GCCCAAGCTTAACAGACTCT+GGG | 0.530545 | 7.4:-35918030 | None:intergenic |
| CGGGTGGCCTGAAATGGTAA+TGG | 0.539649 | 7.4:+35918861 | MS.gene98783:CDS |
| AACAGACTCTGGGTTGTCAA+AGG | 0.543890 | 7.4:-35918020 | None:intergenic |
| GAGCCAGACGCTCAAAACAT+TGG | 0.559739 | 7.4:-35917970 | None:intergenic |
| AAGTCTCTTGCAAATGGAGG+AGG | 0.561240 | 7.4:+35918925 | MS.gene98783:CDS |
| GTAGGTTGTTACTTGGAGGC+GGG | 0.563910 | 7.4:+35918842 | MS.gene98783:CDS |
| GCCTGAAATGGTAATGGCAA+AGG | 0.576296 | 7.4:+35918867 | MS.gene98783:CDS |
| TGGGATAGCCAAGAGTGCTC+GGG | 0.583085 | 7.4:-35918954 | None:intergenic |
| TAACAACCTACTGTCATTGG+CGG | 0.584745 | 7.4:-35918830 | None:intergenic |
| GGTTGTTACTTGGAGGCGGG+TGG | 0.585391 | 7.4:+35918845 | MS.gene98783:CDS |
| ATGAACAAGATAATAGGTCA+TGG | 0.590525 | 7.4:+35917944 | MS.gene98783:CDS |
| TCATTGGCTAGGAAAACTCC+AGG | 0.591835 | 7.4:+35918898 | MS.gene98783:CDS |
| CAAACCAGCATTATCAGCGA+TGG | 0.610258 | 7.4:-35918981 | None:intergenic |
| CAGCCACCATGTTCTTGATG+AGG | 0.618440 | 7.4:+35918765 | MS.gene98783:intron |
| TCTCAGCCTCATCAAGAACA+TGG | 0.644225 | 7.4:-35918771 | None:intergenic |
| CTCAGACCGCCAATGACAGT+AGG | 0.655775 | 7.4:+35918824 | MS.gene98783:CDS |
| ATGTTCTTGATGAGGCTGAG+AGG | 0.658084 | 7.4:+35918773 | MS.gene98783:CDS |
| CGTCTGGCTCTGGTAACTGG+TGG | 0.662358 | 7.4:+35917983 | MS.gene98783:CDS |
| CAGCCTCATCAAGAACATGG+TGG | 0.689750 | 7.4:-35918768 | None:intergenic |
| GGTCTGAGATAGCACACACA+AGG | 0.715225 | 7.4:-35918809 | None:intergenic |
| GCATGCACAATAGTTCTGAG+AGG | 0.722642 | 7.4:+35918127 | MS.gene98783:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGCAGTAATTATTTTTTAAA+AGG | + | chr7.4:35918457-35918476 | MS.gene98783:intron | 15.0% |
| !! | CTTATCGAAGATATTATTAT+TGG | + | chr7.4:35918061-35918080 | MS.gene98783:CDS | 20.0% |
| !! | TATGTTTCATTATCTATTTG+TGG | + | chr7.4:35918296-35918315 | MS.gene98783:intron | 20.0% |
| !! | TCATAGTATTAGTAATTGAT+AGG | + | chr7.4:35918323-35918342 | MS.gene98783:intron | 20.0% |
| !!! | GATATATTGATTAAGTTTTC+TGG | + | chr7.4:35918088-35918107 | MS.gene98783:CDS | 20.0% |
| ! | GATGTTCATTTACATATTCA+AGG | + | chr7.4:35918197-35918216 | MS.gene98783:intron | 25.0% |
| ! | TATGTAAATGAACATCAGAA+AGG | - | chr7.4:35918193-35918212 | None:intergenic | 25.0% |
| !!! | TCATAGCATTTTTTCTTTTG+AGG | + | chr7.4:35918245-35918264 | MS.gene98783:intron | 25.0% |
| ACTGACTAGACCATAAAAAA+TGG | - | chr7.4:35918617-35918636 | None:intergenic | 30.0% | |
| ATGAACAAGATAATAGGTCA+TGG | + | chr7.4:35917944-35917963 | MS.gene98783:CDS | 30.0% | |
| ATTGAGTCCAATGATTTAAC+TGG | + | chr7.4:35918501-35918520 | MS.gene98783:intron | 30.0% | |
| CAGCATAGAAACTGAATATA+AGG | - | chr7.4:35918369-35918388 | None:intergenic | 30.0% | |
| CGAAGATATTATTATTGGTG+AGG | + | chr7.4:35918066-35918085 | MS.gene98783:CDS | 30.0% | |
| CTAGACCATAAAAAATGGTA+AGG | - | chr7.4:35918612-35918631 | None:intergenic | 30.0% | |
| CTTATATTCAGTTTCTATGC+TGG | + | chr7.4:35918367-35918386 | MS.gene98783:intron | 30.0% | |
| !! | TTTCTATGCTGGTTTATTGA+AGG | + | chr7.4:35918378-35918397 | MS.gene98783:intron | 30.0% |
| !!! | CTTTTGAGGTTTTCTTATTC+TGG | + | chr7.4:35918259-35918278 | MS.gene98783:intron | 30.0% |
| AAAAAGTCTCTTGCAAATGG+AGG | + | chr7.4:35918922-35918941 | MS.gene98783:CDS | 35.0% | |
| AAGTAACAACCTACTGTCAT+TGG | - | chr7.4:35918836-35918855 | None:intergenic | 35.0% | |
| AATGACAGTAGGTTGTTACT+TGG | + | chr7.4:35918835-35918854 | MS.gene98783:CDS | 35.0% | |
| CATAAAAAATGGTAAGGAGC+TGG | - | chr7.4:35918606-35918625 | None:intergenic | 35.0% | |
| GGAAAAAAGTCTCTTGCAAA+TGG | + | chr7.4:35918919-35918938 | MS.gene98783:CDS | 35.0% | |
| ! | AGGAAATTGATTCATTGGCT+AGG | + | chr7.4:35918887-35918906 | MS.gene98783:CDS | 35.0% |
| ! | GGCAAAGGAAATTGATTCAT+TGG | + | chr7.4:35918882-35918901 | MS.gene98783:CDS | 35.0% |
| !!! | CAGCTCCTTACCATTTTTTA+TGG | + | chr7.4:35918604-35918623 | MS.gene98783:intron | 35.0% |
| !!! | TTTGCAAGAGACTTTTTTCC+TGG | - | chr7.4:35918919-35918938 | None:intergenic | 35.0% |
| CAGCAGTCCAGTTAAATCAT+TGG | - | chr7.4:35918511-35918530 | None:intergenic | 40.0% | |
| CTGTGACAAAAACAAACGTC+AGG | - | chr7.4:35918748-35918767 | None:intergenic | 40.0% | |
| GCATTTGAGCTGAACATGTA+TGG | + | chr7.4:35918637-35918656 | MS.gene98783:CDS | 40.0% | |
| GTAAGGAGCTGGTGAATATA+AGG | - | chr7.4:35918595-35918614 | None:intergenic | 40.0% | |
| TAACAACCTACTGTCATTGG+CGG | - | chr7.4:35918833-35918852 | None:intergenic | 40.0% | |
| TAACTGGACTGCTGAAATGT+TGG | + | chr7.4:35918517-35918536 | MS.gene98783:intron | 40.0% | |
| TCCTTTGCCATTACCATTTC+AGG | - | chr7.4:35918871-35918890 | None:intergenic | 40.0% | |
| !!! | AGTTTTCTGGTGTTGCAATG+TGG | + | chr7.4:35918101-35918120 | MS.gene98783:CDS | 40.0% |
| AACAGACTCTGGGTTGTCAA+AGG | - | chr7.4:35918023-35918042 | None:intergenic | 45.0% | |
| AACCCAGAGTCTGTTAAGCT+TGG | + | chr7.4:35918028-35918047 | MS.gene98783:CDS | 45.0% | |
| AAGTCTCTTGCAAATGGAGG+AGG | + | chr7.4:35918925-35918944 | MS.gene98783:CDS | 45.0% | |
| ACCCAGAGTCTGTTAAGCTT+GGG | + | chr7.4:35918029-35918048 | MS.gene98783:CDS | 45.0% | |
| ATGTTCTTGATGAGGCTGAG+AGG | + | chr7.4:35918773-35918792 | MS.gene98783:CDS | 45.0% | |
| TCTCAGCCTCATCAAGAACA+TGG | - | chr7.4:35918774-35918793 | None:intergenic | 45.0% | |
| ! | AGTAGGTTGTTACTTGGAGG+CGG | + | chr7.4:35918841-35918860 | MS.gene98783:CDS | 45.0% |
| ! | GACAGTAGGTTGTTACTTGG+AGG | + | chr7.4:35918838-35918857 | MS.gene98783:CDS | 45.0% |
| ! | TCATTGGCTAGGAAAACTCC+AGG | + | chr7.4:35918898-35918917 | MS.gene98783:CDS | 45.0% |
| !! | CATCCAATGTTTTGAGCGTC+TGG | + | chr7.4:35917967-35917986 | MS.gene98783:CDS | 45.0% |
| !! | GCATGCACAATAGTTCTGAG+AGG | + | chr7.4:35918127-35918146 | MS.gene98783:CDS | 45.0% |
| !! | GCCTGAAATGGTAATGGCAA+AGG | + | chr7.4:35918867-35918886 | MS.gene98783:CDS | 45.0% |
| CAGCCACCATGTTCTTGATG+AGG | + | chr7.4:35918765-35918784 | MS.gene98783:intron | 50.0% | |
| CAGCCTCATCAAGAACATGG+TGG | - | chr7.4:35918771-35918790 | None:intergenic | 50.0% | |
| GAGCCAGACGCTCAAAACAT+TGG | - | chr7.4:35917973-35917992 | None:intergenic | 50.0% | |
| GCCCAAGCTTAACAGACTCT+GGG | - | chr7.4:35918033-35918052 | None:intergenic | 50.0% | |
| TGCCCAAGCTTAACAGACTC+TGG | - | chr7.4:35918034-35918053 | None:intergenic | 50.0% | |
| ! | GGTCTGAGATAGCACACACA+AGG | - | chr7.4:35918812-35918831 | None:intergenic | 50.0% |
| ! | GTAGGTTGTTACTTGGAGGC+GGG | + | chr7.4:35918842-35918861 | MS.gene98783:CDS | 50.0% |
| !! | ATGTTTTGAGCGTCTGGCTC+TGG | + | chr7.4:35917973-35917992 | MS.gene98783:CDS | 50.0% |
| !! | TTCTGGTGTTGCAATGTGGC+AGG | + | chr7.4:35918105-35918124 | MS.gene98783:CDS | 50.0% |
| !! | TTGGGATAGCCAAGAGTGCT+CGG | - | chr7.4:35918958-35918977 | None:intergenic | 50.0% |
| CGGGTGGCCTGAAATGGTAA+TGG | + | chr7.4:35918861-35918880 | MS.gene98783:CDS | 55.0% | |
| CTCAGACCGCCAATGACAGT+AGG | + | chr7.4:35918824-35918843 | MS.gene98783:CDS | 55.0% | |
| !! | AGTTCTGAGAGGTGCGTGCT+AGG | + | chr7.4:35918138-35918157 | MS.gene98783:intron | 55.0% |
| !! | TGGGATAGCCAAGAGTGCTC+GGG | - | chr7.4:35918957-35918976 | None:intergenic | 55.0% |
| GGCACTTTCCCGAGCACTCT+TGG | + | chr7.4:35918946-35918965 | MS.gene98783:CDS | 60.0% | |
| ! | GGTTGTTACTTGGAGGCGGG+TGG | + | chr7.4:35918845-35918864 | MS.gene98783:CDS | 60.0% |
| !! | CGTCTGGCTCTGGTAACTGG+TGG | + | chr7.4:35917983-35918002 | MS.gene98783:CDS | 60.0% |
| !! | GAGCGTCTGGCTCTGGTAAC+TGG | + | chr7.4:35917980-35917999 | MS.gene98783:CDS | 60.0% |
| TGGAGGCGGGTGGCCTGAAA+TGG | + | chr7.4:35918855-35918874 | MS.gene98783:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 35917944 | 35918990 | 35917944 | ID=MS.gene98783 |
| chr7.4 | mRNA | 35917944 | 35918990 | 35917944 | ID=MS.gene98783.t1;Parent=MS.gene98783 |
| chr7.4 | exon | 35917944 | 35918148 | 35917944 | ID=MS.gene98783.t1.exon1;Parent=MS.gene98783.t1 |
| chr7.4 | CDS | 35917944 | 35918148 | 35917944 | ID=cds.MS.gene98783.t1;Parent=MS.gene98783.t1 |
| chr7.4 | exon | 35918632 | 35918671 | 35918632 | ID=MS.gene98783.t1.exon2;Parent=MS.gene98783.t1 |
| chr7.4 | CDS | 35918632 | 35918671 | 35918632 | ID=cds.MS.gene98783.t1;Parent=MS.gene98783.t1 |
| chr7.4 | exon | 35918768 | 35918990 | 35918768 | ID=MS.gene98783.t1.exon3;Parent=MS.gene98783.t1 |
| chr7.4 | CDS | 35918768 | 35918990 | 35918768 | ID=cds.MS.gene98783.t1;Parent=MS.gene98783.t1 |
| Gene Sequence |
| Protein sequence |
