Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056642.t1 | KEH30371.1 | 75 | 48 | 12 | 0 | 14 | 61 | 562 | 609 | 8.00E-07 | 63.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056642.t1 | A0A072ULY9 | 75.0 | 48 | 12 | 0 | 14 | 61 | 562 | 609 | 5.8e-07 | 63.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049825 | MS.gene056642 | 0.816386 | 5.68E-52 | -1.69E-46 |
| MS.gene049873 | MS.gene056642 | 0.841678 | 4.11E-58 | -1.69E-46 |
| MS.gene052358 | MS.gene056642 | -0.813651 | 2.30E-51 | -1.69E-46 |
| MS.gene052519 | MS.gene056642 | 0.813883 | 2.04E-51 | -1.69E-46 |
| MS.gene056642 | MS.gene057060 | 0.803804 | 2.93E-49 | -1.69E-46 |
| MS.gene056642 | MS.gene058625 | 0.805861 | 1.09E-49 | -1.69E-46 |
| MS.gene056642 | MS.gene05954 | 0.831383 | 1.72E-55 | -1.69E-46 |
| MS.gene056642 | MS.gene060429 | 0.814047 | 1.88E-51 | -1.69E-46 |
| MS.gene056642 | MS.gene060572 | 0.811561 | 6.58E-51 | -1.69E-46 |
| MS.gene056642 | MS.gene061900 | 0.814007 | 1.92E-51 | -1.69E-46 |
| MS.gene056642 | MS.gene062672 | 0.806685 | 7.30E-50 | -1.69E-46 |
| MS.gene056642 | MS.gene062743 | 0.814042 | 1.88E-51 | -1.69E-46 |
| MS.gene056642 | MS.gene064944 | 0.81027 | 1.25E-50 | -1.69E-46 |
| MS.gene056642 | MS.gene066267 | 0.806355 | 8.56E-50 | -1.69E-46 |
| MS.gene056642 | MS.gene071570 | 0.844634 | 6.69E-59 | -1.69E-46 |
| MS.gene056642 | MS.gene072942 | 0.81429 | 1.66E-51 | -1.69E-46 |
| MS.gene056642 | MS.gene073301 | 0.813108 | 3.02E-51 | -1.69E-46 |
| MS.gene056642 | MS.gene074606 | 0.807095 | 5.98E-50 | -1.69E-46 |
| MS.gene056642 | MS.gene08365 | 0.800012 | 1.76E-48 | -1.69E-46 |
| MS.gene056642 | MS.gene08553 | 0.815871 | 7.40E-52 | -1.69E-46 |
| MS.gene056642 | MS.gene08554 | 0.84196 | 3.46E-58 | -1.69E-46 |
| MS.gene056642 | MS.gene08882 | 0.802835 | 4.65E-49 | -1.69E-46 |
| MS.gene056642 | MS.gene21928 | 0.802074 | 6.67E-49 | -1.69E-46 |
| MS.gene056642 | MS.gene22270 | 0.813159 | 2.95E-51 | -1.69E-46 |
| MS.gene056642 | MS.gene24623 | 0.822279 | 2.58E-53 | -1.69E-46 |
| MS.gene056642 | MS.gene24626 | 0.805427 | 1.34E-49 | -1.69E-46 |
| MS.gene056642 | MS.gene24628 | 0.813585 | 2.38E-51 | -1.69E-46 |
| MS.gene056642 | MS.gene24692 | 0.807532 | 4.83E-50 | -1.69E-46 |
| MS.gene056642 | MS.gene25755 | 0.803694 | 3.09E-49 | -1.69E-46 |
| MS.gene056642 | MS.gene28652 | 0.815254 | 1.02E-51 | -1.69E-46 |
| MS.gene056642 | MS.gene33131 | 0.808812 | 2.58E-50 | -1.69E-46 |
| MS.gene056642 | MS.gene34979 | 0.803171 | 3.96E-49 | -1.69E-46 |
| MS.gene056642 | MS.gene35135 | 0.805846 | 1.10E-49 | -1.69E-46 |
| MS.gene056642 | MS.gene36496 | 0.822054 | 2.91E-53 | -1.69E-46 |
| MS.gene056642 | MS.gene37027 | 0.801843 | 7.44E-49 | -1.69E-46 |
| MS.gene056642 | MS.gene40022 | 0.810107 | 1.36E-50 | -1.69E-46 |
| MS.gene056642 | MS.gene40811 | 0.804105 | 2.53E-49 | -1.69E-46 |
| MS.gene056642 | MS.gene42162 | 0.806926 | 6.49E-50 | -1.69E-46 |
| MS.gene056642 | MS.gene43992 | 0.815151 | 1.07E-51 | -1.69E-46 |
| MS.gene056642 | MS.gene51106 | -0.819235 | 1.29E-52 | -1.69E-46 |
| MS.gene056642 | MS.gene52940 | 0.802839 | 4.64E-49 | -1.69E-46 |
| MS.gene056642 | MS.gene53586 | 0.810697 | 1.01E-50 | -1.69E-46 |
| MS.gene056642 | MS.gene56468 | 0.819094 | 1.39E-52 | -1.69E-46 |
| MS.gene056642 | MS.gene59369 | 0.801301 | 9.61E-49 | -1.69E-46 |
| MS.gene056642 | MS.gene64249 | 0.817356 | 3.44E-52 | -1.69E-46 |
| MS.gene056642 | MS.gene67746 | 0.812491 | 4.13E-51 | -1.69E-46 |
| MS.gene056642 | MS.gene68390 | 0.815517 | 8.88E-52 | -1.69E-46 |
| MS.gene056642 | MS.gene69947 | 0.804102 | 2.54E-49 | -1.69E-46 |
| MS.gene056642 | MS.gene70850 | 0.808866 | 2.51E-50 | -1.69E-46 |
| MS.gene056642 | MS.gene72121 | 0.800954 | 1.13E-48 | -1.69E-46 |
| MS.gene056642 | MS.gene72123 | 0.804982 | 1.66E-49 | -1.69E-46 |
| MS.gene056642 | MS.gene72268 | 0.807812 | 4.21E-50 | -1.69E-46 |
| MS.gene056642 | MS.gene72347 | 0.805272 | 1.45E-49 | -1.69E-46 |
| MS.gene056642 | MS.gene75003 | 0.820398 | 7.00E-53 | -1.69E-46 |
| MS.gene056642 | MS.gene76118 | -0.818726 | 1.69E-52 | -1.69E-46 |
| MS.gene056642 | MS.gene77588 | 0.80319 | 3.92E-49 | -1.69E-46 |
| MS.gene056642 | MS.gene79112 | 0.800619 | 1.32E-48 | -1.69E-46 |
| MS.gene056642 | MS.gene79960 | 0.801118 | 1.05E-48 | -1.69E-46 |
| MS.gene056642 | MS.gene81375 | 0.820603 | 6.29E-53 | -1.69E-46 |
| MS.gene056642 | MS.gene89036 | 0.807706 | 4.43E-50 | -1.69E-46 |
| MS.gene056642 | MS.gene98783 | 0.801223 | 9.97E-49 | -1.69E-46 |
| MS.gene056642 | MS.gene99657 | 0.820776 | 5.74E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTTTGATAATTCCAAATTT+TGG | 0.177386 | 4.3:-36391037 | None:intergenic |
| TGGAATTATCAAACTTCATT+TGG | 0.241574 | 4.3:+36391045 | MS.gene056642:CDS |
| GATGCGGTGGTTGATTTCTT+TGG | 0.258076 | 4.3:-36390848 | None:intergenic |
| GGAATTATCAAACTTCATTT+GGG | 0.304283 | 4.3:+36391046 | MS.gene056642:CDS |
| CCTGTGAAGCGTCCAAAATT+TGG | 0.307340 | 4.3:+36391025 | MS.gene056642:CDS |
| CTAATAATTAGTACAATATA+TGG | 0.338634 | 4.3:-36391123 | None:intergenic |
| CTCCAAGGCTGCTGCTGTGT+TGG | 0.359397 | 4.3:+36390925 | MS.gene056642:CDS |
| ATCATCCGCCACCTCGATCT+TGG | 0.367746 | 4.3:-36390894 | None:intergenic |
| CTCCAACACAGCAGCAGCCT+TGG | 0.406523 | 4.3:-36390927 | None:intergenic |
| TTCTCATTCTCATCGTCCTC+TGG | 0.438046 | 4.3:+36390964 | MS.gene056642:CDS |
| ATATGGTAATAAACTAAACT+AGG | 0.518565 | 4.3:-36391106 | None:intergenic |
| TTGGAAGAAGTTATAACAGC+AGG | 0.519988 | 4.3:-36390875 | None:intergenic |
| TTCTTCCAAGATCGAGGTGG+CGG | 0.539481 | 4.3:+36390889 | MS.gene056642:CDS |
| TGATTTCTTTGGATTTGACA+TGG | 0.571108 | 4.3:-36390837 | None:intergenic |
| AACAGCAGGTTGAGATGCGG+TGG | 0.585201 | 4.3:-36390861 | None:intergenic |
| AGCCGCAGCATCAGTAGAGG+TGG | 0.588941 | 4.3:-36390996 | None:intergenic |
| TTCCACCTCTACTGATGCTG+CGG | 0.591522 | 4.3:+36390994 | MS.gene056642:CDS |
| GGATGATTTATCTTTCTCCA+AGG | 0.593081 | 4.3:+36390910 | MS.gene056642:CDS |
| GAATTATCAAACTTCATTTG+GGG | 0.621507 | 4.3:+36391047 | MS.gene056642:CDS |
| TATAACTTCTTCCAAGATCG+AGG | 0.626622 | 4.3:+36390883 | MS.gene056642:CDS |
| CTTAGCCGCAGCATCAGTAG+AGG | 0.632027 | 4.3:-36390999 | None:intergenic |
| AACTTCTTCCAAGATCGAGG+TGG | 0.689328 | 4.3:+36390886 | MS.gene056642:CDS |
| TATAACAGCAGGTTGAGATG+CGG | 0.689445 | 4.3:-36390864 | None:intergenic |
| GAGGTGGAACACTTAACCAG+AGG | 0.716047 | 4.3:-36390980 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATGGTAATAAACTAAACT+AGG | - | chr4.3:36391109-36391128 | None:intergenic | 20.0% |
| !!! | AGTTTGATAATTCCAAATTT+TGG | - | chr4.3:36391040-36391059 | None:intergenic | 20.0% |
| ! | GAATTATCAAACTTCATTTG+GGG | + | chr4.3:36391047-36391066 | MS.gene056642:CDS | 25.0% |
| ! | GGAATTATCAAACTTCATTT+GGG | + | chr4.3:36391046-36391065 | MS.gene056642:CDS | 25.0% |
| ! | TGGAATTATCAAACTTCATT+TGG | + | chr4.3:36391045-36391064 | MS.gene056642:CDS | 25.0% |
| GGATGATTTATCTTTCTCCA+AGG | + | chr4.3:36390910-36390929 | MS.gene056642:CDS | 35.0% | |
| TATAACTTCTTCCAAGATCG+AGG | + | chr4.3:36390883-36390902 | MS.gene056642:CDS | 35.0% | |
| TTGGAAGAAGTTATAACAGC+AGG | - | chr4.3:36390878-36390897 | None:intergenic | 35.0% | |
| ! | TATAACAGCAGGTTGAGATG+CGG | - | chr4.3:36390867-36390886 | None:intergenic | 40.0% |
| AACTTCTTCCAAGATCGAGG+TGG | + | chr4.3:36390886-36390905 | MS.gene056642:CDS | 45.0% | |
| TTCTCATTCTCATCGTCCTC+TGG | + | chr4.3:36390964-36390983 | MS.gene056642:CDS | 45.0% | |
| ! | CCAAATTTTGGACGCTTCAC+AGG | - | chr4.3:36391028-36391047 | None:intergenic | 45.0% |
| ! | CCTGTGAAGCGTCCAAAATT+TGG | + | chr4.3:36391025-36391044 | MS.gene056642:CDS | 45.0% |
| ! | GATGCGGTGGTTGATTTCTT+TGG | - | chr4.3:36390851-36390870 | None:intergenic | 45.0% |
| GAGGTGGAACACTTAACCAG+AGG | - | chr4.3:36390983-36391002 | None:intergenic | 50.0% | |
| TTCCACCTCTACTGATGCTG+CGG | + | chr4.3:36390994-36391013 | MS.gene056642:CDS | 50.0% | |
| TTCTTCCAAGATCGAGGTGG+CGG | + | chr4.3:36390889-36390908 | MS.gene056642:CDS | 50.0% | |
| ATCATCCGCCACCTCGATCT+TGG | - | chr4.3:36390897-36390916 | None:intergenic | 55.0% | |
| CTTAGCCGCAGCATCAGTAG+AGG | - | chr4.3:36391002-36391021 | None:intergenic | 55.0% | |
| ! | AACAGCAGGTTGAGATGCGG+TGG | - | chr4.3:36390864-36390883 | None:intergenic | 55.0% |
| AGCCGCAGCATCAGTAGAGG+TGG | - | chr4.3:36390999-36391018 | None:intergenic | 60.0% | |
| CTCCAACACAGCAGCAGCCT+TGG | - | chr4.3:36390930-36390949 | None:intergenic | 60.0% | |
| CTCCAAGGCTGCTGCTGTGT+TGG | + | chr4.3:36390925-36390944 | MS.gene056642:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 36390839 | 36391138 | 36390839 | ID=MS.gene056642 |
| chr4.3 | mRNA | 36390839 | 36391138 | 36390839 | ID=MS.gene056642.t1;Parent=MS.gene056642 |
| chr4.3 | exon | 36390839 | 36391138 | 36390839 | ID=MS.gene056642.t1.exon1;Parent=MS.gene056642.t1 |
| chr4.3 | CDS | 36390839 | 36391138 | 36390839 | ID=cds.MS.gene056642.t1;Parent=MS.gene056642.t1 |
| Gene Sequence |
| Protein sequence |
