Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048038.01.T01 | XP_003609015.1 | 97.419 | 310 | 8 | 0 | 1 | 310 | 36 | 345 | 0 | 634 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048038.01.T01 | A2XQE8 | 62.821 | 312 | 109 | 4 | 1 | 310 | 33 | 339 | 3.00E-145 | 415 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048038.01.T01 | G7JPY3 | 97.419 | 310 | 8 | 0 | 1 | 310 | 36 | 345 | 0.0 | 634 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048038.01 | MsG0880046427.01 | 0.951396 | 2.231183e-109 | 5.074671e-104 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048038.01.T01 | MTR_4g107930 | 97.419 | 310 | 8 | 0 | 1 | 310 | 36 | 345 | 0.0 | 634 |
| MsG0080048038.01.T01 | MTR_4g079770 | 91.935 | 310 | 24 | 1 | 1 | 310 | 37 | 345 | 0.0 | 597 |
| MsG0080048038.01.T01 | MTR_4g079470 | 91.935 | 310 | 24 | 1 | 1 | 310 | 37 | 345 | 0.0 | 597 |
| MsG0080048038.01.T01 | MTR_4g047610 | 90.645 | 310 | 29 | 0 | 1 | 310 | 34 | 343 | 0.0 | 595 |
| MsG0080048038.01.T01 | MTR_5g022560 | 90.645 | 310 | 29 | 0 | 1 | 310 | 35 | 344 | 0.0 | 592 |
| MsG0080048038.01.T01 | MTR_4g079800 | 88.387 | 310 | 35 | 1 | 1 | 310 | 37 | 345 | 0.0 | 576 |
| MsG0080048038.01.T01 | MTR_2g075830 | 79.032 | 310 | 62 | 2 | 1 | 310 | 35 | 341 | 1.14e-176 | 492 |
| MsG0080048038.01.T01 | MTR_4g080700 | 66.452 | 310 | 99 | 4 | 1 | 310 | 36 | 340 | 3.52e-152 | 430 |
| MsG0080048038.01.T01 | MTR_4g080730 | 66.452 | 310 | 99 | 4 | 1 | 310 | 36 | 340 | 3.04e-151 | 427 |
| MsG0080048038.01.T01 | MTR_4g080360 | 56.958 | 309 | 129 | 3 | 4 | 310 | 36 | 342 | 5.51e-129 | 371 |
| MsG0080048038.01.T01 | MTR_4g104370 | 59.615 | 312 | 117 | 7 | 1 | 308 | 36 | 342 | 1.18e-127 | 369 |
| MsG0080048038.01.T01 | MTR_2g083930 | 54.313 | 313 | 137 | 2 | 1 | 308 | 31 | 342 | 3.11e-126 | 364 |
| MsG0080048038.01.T01 | MTR_4g079440 | 56.634 | 309 | 126 | 5 | 1 | 308 | 42 | 343 | 3.97e-123 | 356 |
| MsG0080048038.01.T01 | MTR_3g075390 | 54.747 | 316 | 127 | 5 | 1 | 308 | 36 | 343 | 8.28e-123 | 356 |
| MsG0080048038.01.T01 | MTR_7g078600 | 57.282 | 309 | 121 | 6 | 1 | 308 | 38 | 336 | 1.68e-120 | 350 |
| MsG0080048038.01.T01 | MTR_2g090890 | 53.722 | 309 | 136 | 4 | 1 | 309 | 35 | 336 | 3.62e-118 | 343 |
| MsG0080048038.01.T01 | MTR_7g079230 | 55.519 | 308 | 129 | 6 | 1 | 308 | 35 | 334 | 4.00e-117 | 341 |
| MsG0080048038.01.T01 | MTR_1g107550 | 55.414 | 314 | 129 | 7 | 1 | 308 | 46 | 354 | 1.89e-116 | 344 |
| MsG0080048038.01.T01 | MTR_3g116080 | 54.313 | 313 | 131 | 6 | 1 | 309 | 43 | 347 | 4.06e-116 | 339 |
| MsG0080048038.01.T01 | MTR_4g094918 | 51.768 | 311 | 140 | 6 | 5 | 309 | 35 | 341 | 1.82e-112 | 330 |
| MsG0080048038.01.T01 | MTR_1g018840 | 52.716 | 313 | 132 | 6 | 5 | 308 | 55 | 360 | 2.95e-110 | 328 |
| MsG0080048038.01.T01 | MTR_1g018840 | 52.716 | 313 | 132 | 6 | 5 | 308 | 55 | 360 | 5.22e-110 | 328 |
| MsG0080048038.01.T01 | MTR_3g102220 | 52.063 | 315 | 133 | 7 | 5 | 308 | 53 | 360 | 1.67e-109 | 326 |
| MsG0080048038.01.T01 | MTR_3g112430 | 50.482 | 311 | 141 | 5 | 6 | 309 | 37 | 341 | 4.29e-102 | 306 |
| MsG0080048038.01.T01 | MTR_2g015850 | 53.793 | 290 | 127 | 5 | 21 | 308 | 78 | 362 | 3.05e-98 | 294 |
| MsG0080048038.01.T01 | MTR_2g016180 | 47.619 | 315 | 149 | 8 | 3 | 309 | 49 | 355 | 1.40e-89 | 276 |
| MsG0080048038.01.T01 | MTR_2g016270 | 47.436 | 312 | 139 | 8 | 3 | 309 | 49 | 340 | 8.87e-88 | 271 |
| MsG0080048038.01.T01 | MTR_2g067900 | 46.688 | 317 | 148 | 12 | 4 | 307 | 15 | 323 | 2.89e-79 | 244 |
| MsG0080048038.01.T01 | MTR_2g067560 | 45.652 | 322 | 142 | 14 | 4 | 304 | 15 | 324 | 4.65e-73 | 241 |
| MsG0080048038.01.T01 | MTR_2g064475 | 41.714 | 350 | 147 | 17 | 4 | 309 | 15 | 351 | 6.47e-73 | 229 |
| MsG0080048038.01.T01 | MTR_7g078630 | 46.403 | 278 | 107 | 7 | 3 | 269 | 704 | 950 | 1.08e-70 | 236 |
| MsG0080048038.01.T01 | MTR_4g125300 | 42.071 | 309 | 160 | 10 | 7 | 309 | 53 | 348 | 4.96e-69 | 219 |
| MsG0080048038.01.T01 | MTR_2g015920 | 40.317 | 315 | 173 | 11 | 3 | 309 | 51 | 358 | 7.84e-68 | 216 |
| MsG0080048038.01.T01 | MTR_4g065077 | 38.750 | 320 | 175 | 10 | 1 | 309 | 47 | 356 | 2.36e-65 | 209 |
| MsG0080048038.01.T01 | MTR_2g015930 | 39.623 | 318 | 172 | 11 | 3 | 309 | 50 | 358 | 6.79e-65 | 208 |
| MsG0080048038.01.T01 | MTR_2g066980 | 38.154 | 325 | 141 | 12 | 5 | 309 | 34 | 318 | 4.94e-64 | 205 |
| MsG0080048038.01.T01 | MTR_4g071060 | 39.871 | 311 | 161 | 9 | 11 | 306 | 54 | 353 | 1.81e-63 | 204 |
| MsG0080048038.01.T01 | MTR_1g023210 | 39.048 | 315 | 151 | 11 | 10 | 301 | 56 | 352 | 2.21e-62 | 202 |
| MsG0080048038.01.T01 | MTR_1g023810 | 44.635 | 233 | 120 | 6 | 78 | 307 | 35 | 261 | 5.60e-62 | 198 |
| MsG0080048038.01.T01 | MTR_1g094135 | 36.957 | 322 | 173 | 8 | 3 | 306 | 54 | 363 | 9.26e-60 | 196 |
| MsG0080048038.01.T01 | MTR_8g086470 | 37.107 | 318 | 167 | 9 | 1 | 309 | 63 | 356 | 1.53e-59 | 194 |
| MsG0080048038.01.T01 | MTR_4g072790 | 34.202 | 307 | 156 | 10 | 7 | 306 | 50 | 317 | 5.15e-44 | 153 |
| MsG0080048038.01.T01 | MTR_7g078580 | 64.286 | 98 | 34 | 1 | 211 | 308 | 4 | 100 | 6.97e-42 | 141 |
| MsG0080048038.01.T01 | MTR_7g079130 | 69.231 | 91 | 28 | 0 | 218 | 308 | 6 | 96 | 7.10e-41 | 138 |
| MsG0080048038.01.T01 | MTR_4g072340 | 33.010 | 309 | 161 | 10 | 7 | 306 | 50 | 321 | 2.29e-39 | 141 |
| MsG0080048038.01.T01 | MTR_4g073660 | 33.657 | 309 | 159 | 10 | 7 | 306 | 49 | 320 | 2.41e-39 | 141 |
| MsG0080048038.01.T01 | MTR_2g015840 | 51.969 | 127 | 58 | 2 | 3 | 127 | 31 | 156 | 7.32e-37 | 133 |
| MsG0080048038.01.T01 | MTR_2g015840 | 56.250 | 112 | 48 | 1 | 198 | 309 | 161 | 271 | 8.39e-37 | 133 |
| MsG0080048038.01.T01 | MTR_1g025450 | 39.891 | 183 | 83 | 8 | 86 | 262 | 47 | 208 | 1.05e-31 | 124 |
| MsG0080048038.01.T01 | MTR_7g078310 | 43.571 | 140 | 70 | 2 | 48 | 185 | 115 | 247 | 1.13e-28 | 111 |
| MsG0080048038.01.T01 | MTR_2g016380 | 46.281 | 121 | 58 | 4 | 193 | 309 | 18 | 135 | 6.19e-27 | 106 |
| MsG0080048038.01.T01 | MTR_7g111060 | 26.071 | 280 | 172 | 8 | 44 | 301 | 64 | 330 | 9.03e-23 | 97.1 |
| MsG0080048038.01.T01 | MTR_7g079260 | 59.722 | 72 | 13 | 1 | 235 | 306 | 7 | 62 | 5.61e-22 | 89.0 |
| MsG0080048038.01.T01 | MTR_7g111050 | 30.085 | 236 | 133 | 9 | 98 | 309 | 110 | 337 | 7.14e-22 | 94.7 |
| MsG0080048038.01.T01 | MTR_3g029560 | 27.200 | 250 | 150 | 8 | 64 | 309 | 16 | 237 | 3.82e-21 | 90.5 |
| MsG0080048038.01.T01 | MTR_8g087510 | 41.985 | 131 | 69 | 5 | 133 | 261 | 23 | 148 | 7.97e-21 | 88.2 |
| MsG0080048038.01.T01 | MTR_2g047960 | 49.412 | 85 | 42 | 1 | 101 | 185 | 7 | 90 | 3.31e-19 | 81.3 |
| MsG0080048038.01.T01 | MTR_3g082370 | 26.442 | 208 | 131 | 7 | 97 | 288 | 5 | 206 | 1.59e-16 | 77.8 |
| MsG0080048038.01.T01 | MTR_7g111050 | 28.061 | 196 | 115 | 7 | 98 | 271 | 105 | 296 | 1.17e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048038.01.T01 | AT5G45890 | 58.599 | 314 | 123 | 3 | 1 | 309 | 34 | 345 | 2.61e-134 | 385 |
| MsG0080048038.01.T01 | AT5G50260 | 57.278 | 316 | 119 | 5 | 1 | 308 | 34 | 341 | 2.55e-127 | 368 |
| MsG0080048038.01.T01 | AT3G48340 | 57.692 | 312 | 117 | 6 | 5 | 308 | 38 | 342 | 1.69e-122 | 355 |
| MsG0080048038.01.T01 | AT4G35350 | 53.355 | 313 | 135 | 5 | 1 | 309 | 47 | 352 | 8.89e-118 | 343 |
| MsG0080048038.01.T01 | AT2G34080 | 53.016 | 315 | 139 | 5 | 1 | 310 | 35 | 345 | 1.43e-117 | 342 |
| MsG0080048038.01.T01 | AT3G49340 | 51.735 | 317 | 136 | 6 | 3 | 310 | 33 | 341 | 2.18e-115 | 337 |
| MsG0080048038.01.T01 | AT2G27420 | 51.724 | 319 | 140 | 5 | 3 | 310 | 33 | 348 | 2.43e-114 | 334 |
| MsG0080048038.01.T01 | AT3G48350 | 54.459 | 314 | 134 | 6 | 1 | 309 | 34 | 343 | 1.01e-113 | 333 |
| MsG0080048038.01.T01 | AT3G19390 | 52.903 | 310 | 139 | 5 | 1 | 309 | 42 | 345 | 2.80e-112 | 333 |
| MsG0080048038.01.T01 | AT5G43060 | 53.871 | 310 | 130 | 6 | 5 | 308 | 50 | 352 | 2.04e-111 | 331 |
| MsG0080048038.01.T01 | AT1G20850 | 52.090 | 311 | 143 | 4 | 1 | 309 | 47 | 353 | 1.01e-110 | 325 |
| MsG0080048038.01.T01 | AT4G36880 | 52.077 | 313 | 138 | 6 | 7 | 309 | 51 | 361 | 1.99e-110 | 325 |
| MsG0080048038.01.T01 | AT3G19400 | 50.814 | 307 | 147 | 4 | 5 | 309 | 44 | 348 | 1.21e-109 | 323 |
| MsG0080048038.01.T01 | AT1G06260 | 53.871 | 310 | 134 | 5 | 1 | 308 | 39 | 341 | 1.15e-108 | 320 |
| MsG0080048038.01.T01 | AT1G29090 | 49.367 | 316 | 147 | 6 | 3 | 310 | 45 | 355 | 4.17e-108 | 318 |
| MsG0080048038.01.T01 | AT1G47128 | 50.809 | 309 | 140 | 7 | 5 | 308 | 50 | 351 | 7.90e-107 | 319 |
| MsG0080048038.01.T01 | AT1G29080 | 49.359 | 312 | 148 | 6 | 5 | 310 | 39 | 346 | 2.52e-105 | 311 |
| MsG0080048038.01.T01 | AT1G09850 | 50.000 | 306 | 150 | 3 | 3 | 308 | 30 | 332 | 1.12e-101 | 305 |
| MsG0080048038.01.T01 | AT4G23520 | 49.191 | 309 | 143 | 9 | 6 | 308 | 48 | 348 | 3.32e-99 | 296 |
| MsG0080048038.01.T01 | AT4G11310 | 47.896 | 309 | 148 | 6 | 6 | 308 | 50 | 351 | 1.07e-98 | 295 |
| MsG0080048038.01.T01 | AT4G11320 | 47.249 | 309 | 150 | 6 | 6 | 308 | 57 | 358 | 5.64e-98 | 293 |
| MsG0080048038.01.T01 | AT4G11320 | 47.249 | 309 | 150 | 6 | 6 | 308 | 57 | 358 | 5.64e-98 | 293 |
| MsG0080048038.01.T01 | AT1G29110 | 44.872 | 312 | 149 | 7 | 5 | 309 | 38 | 333 | 8.79e-88 | 266 |
| MsG0080048038.01.T01 | AT3G43960 | 46.452 | 310 | 155 | 8 | 5 | 308 | 41 | 345 | 1.65e-86 | 264 |
| MsG0080048038.01.T01 | AT4G35350 | 50.607 | 247 | 111 | 5 | 1 | 243 | 47 | 286 | 4.56e-83 | 253 |
| MsG0080048038.01.T01 | AT3G19400 | 48.963 | 241 | 120 | 3 | 5 | 243 | 44 | 283 | 8.84e-76 | 234 |
| MsG0080048038.01.T01 | AT3G48350 | 50.806 | 248 | 113 | 6 | 1 | 243 | 34 | 277 | 3.69e-74 | 230 |
| MsG0080048038.01.T01 | AT5G60360 | 43.042 | 309 | 157 | 11 | 7 | 309 | 61 | 356 | 9.99e-72 | 226 |
| MsG0080048038.01.T01 | AT5G60360 | 42.718 | 309 | 157 | 12 | 7 | 309 | 61 | 355 | 3.58e-69 | 219 |
| MsG0080048038.01.T01 | AT3G45310 | 41.613 | 310 | 162 | 11 | 7 | 310 | 61 | 357 | 2.30e-68 | 217 |
| MsG0080048038.01.T01 | AT5G60360 | 42.333 | 300 | 154 | 11 | 7 | 300 | 61 | 347 | 3.50e-67 | 214 |
| MsG0080048038.01.T01 | AT3G45310 | 41.290 | 310 | 162 | 12 | 7 | 310 | 61 | 356 | 8.25e-66 | 211 |
| MsG0080048038.01.T01 | AT4G39090 | 39.365 | 315 | 158 | 11 | 11 | 306 | 57 | 357 | 6.11e-63 | 203 |
| MsG0080048038.01.T01 | AT2G21430 | 37.908 | 306 | 165 | 9 | 11 | 301 | 54 | 349 | 2.47e-61 | 199 |
| MsG0080048038.01.T01 | AT3G54940 | 37.695 | 321 | 163 | 10 | 8 | 306 | 54 | 359 | 5.27e-58 | 191 |
| MsG0080048038.01.T01 | AT4G16190 | 38.095 | 315 | 166 | 10 | 10 | 306 | 60 | 363 | 2.69e-57 | 189 |
| MsG0080048038.01.T01 | AT3G54940 | 37.991 | 229 | 125 | 5 | 8 | 225 | 54 | 276 | 1.51e-42 | 148 |
| MsG0080048038.01.T01 | AT1G02305 | 29.778 | 225 | 130 | 7 | 98 | 301 | 116 | 333 | 1.81e-23 | 99.4 |
| MsG0080048038.01.T01 | AT4G01610 | 26.923 | 286 | 177 | 9 | 41 | 301 | 52 | 330 | 5.26e-22 | 95.1 |
| MsG0080048038.01.T01 | AT4G01610 | 26.573 | 286 | 178 | 9 | 41 | 301 | 52 | 330 | 1.04e-20 | 91.7 |
| MsG0080048038.01.T01 | AT1G02300 | 28.910 | 211 | 124 | 6 | 111 | 301 | 146 | 350 | 3.61e-19 | 87.4 |
| MsG0080048038.01.T01 | AT2G22160 | 39.130 | 92 | 54 | 1 | 20 | 111 | 9 | 98 | 2.09e-13 | 65.9 |
Find 84 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATCAATTGCAACAGAAAT+AGG | 0.303591 | contig179end:-34872 | None:intergenic |
| TCAATATGGCAAAGTCTATA+AGG | 0.330619 | contig179end:+34131 | MsG0080048038.01.T01:CDS |
| TACAAAAGTGGTGTCTTTAC+CGG | 0.332301 | contig179end:+34917 | MsG0080048038.01.T01:CDS |
| AGGTTGTGAGGGTGGTCTTA+TGG | 0.350865 | contig179end:+34667 | MsG0080048038.01.T01:CDS |
| AAATTCATTATTCAAAATCA+TGG | 0.369502 | contig179end:+34701 | MsG0080048038.01.T01:CDS |
| TTGCAATGCCTGCTCATTGT+TGG | 0.384601 | contig179end:-34831 | None:intergenic |
| ATTCATGCAGCTACCATTAC+CGG | 0.385380 | contig179end:+34791 | MsG0080048038.01.T01:CDS |
| TACCCTTATCAAGGTGTTGA+TGG | 0.427747 | contig179end:+34743 | MsG0080048038.01.T01:CDS |
| AATAACAAGCTATACAAGTT+AGG | 0.429513 | contig179end:+34231 | MsG0080048038.01.T01:CDS |
| GGAATTAATAAGTTGAGTAC+TGG | 0.441256 | contig179end:+34584 | MsG0080048038.01.T01:CDS |
| GGAACTGAGTGGGGTGAAGA+AGG | 0.443728 | contig179end:+35034 | MsG0080048038.01.T01:CDS |
| GAGCAATGGATGAGTCAATA+TGG | 0.445016 | contig179end:+34117 | MsG0080048038.01.T01:CDS |
| TTCCATCAACACCTTGATAA+GGG | 0.450919 | contig179end:-34745 | None:intergenic |
| AATGCCTGCTCATTGTTGGC+AGG | 0.451756 | contig179end:-34827 | None:intergenic |
| GTTCCATCAACACCTTGATA+AGG | 0.464383 | contig179end:-34746 | None:intergenic |
| CAATCCCGTCCGTTGTGGAT+TGG | 0.469084 | contig179end:+34373 | MsG0080048038.01.T01:CDS |
| GTTCTTAACCAACCAATACT+TGG | 0.469107 | contig179end:-35005 | None:intergenic |
| AAGTTTGACAATTAAGCAGT+AGG | 0.473215 | contig179end:-35127 | None:intergenic |
| TAAGTTGAGTACTGGAAAGT+TGG | 0.474031 | contig179end:+34592 | MsG0080048038.01.T01:CDS |
| GGTAATGGTAGCTGCATGAA+TGG | 0.481667 | contig179end:-34789 | None:intergenic |
| AGCATCTAGAAATAAATTCA+AGG | 0.484430 | contig179end:+34293 | MsG0080048038.01.T01:CDS |
| AGATGCTATGAACTCCTCAT+TGG | 0.488280 | contig179end:-34277 | None:intergenic |
| CATTGACCTTGATTCTTCAC+AGG | 0.495833 | contig179end:-34417 | None:intergenic |
| ATTGGTTGGTTAAGAACTCA+TGG | 0.497126 | contig179end:+35011 | MsG0080048038.01.T01:CDS |
| CAACTCGGTTCCACATGAAC+CGG | 0.497716 | contig179end:-34936 | None:intergenic |
| GGGGTGAAGAAGGATACATT+AGG | 0.500551 | contig179end:+35044 | MsG0080048038.01.T01:CDS |
| GGTGTCACCGCTGTAGGTTA+TGG | 0.501955 | contig179end:+34965 | MsG0080048038.01.T01:CDS |
| AGGATACATTAGGATGCAAA+GGG | 0.504604 | contig179end:+35054 | MsG0080048038.01.T01:CDS |
| TCTATAAGGATCCTCAAGAA+AGG | 0.505398 | contig179end:+34145 | MsG0080048038.01.T01:CDS |
| TCCCGTCCGTTGTGGATTGG+AGG | 0.514340 | contig179end:+34376 | MsG0080048038.01.T01:CDS |
| GGTTATGGTGTCGGTAATGA+TGG | 0.515702 | contig179end:+34980 | MsG0080048038.01.T01:CDS |
| CTATAAGGATCCTCAAGAAA+GGG | 0.516295 | contig179end:+34146 | MsG0080048038.01.T01:CDS |
| AGACGTTCCTCCCTTTCTTG+AGG | 0.517763 | contig179end:-34156 | None:intergenic |
| GACATCTTCATACCCGGTAA+TGG | 0.518079 | contig179end:-34804 | None:intergenic |
| TTGCTCGTGCCTCTCATACA+TGG | 0.519986 | contig179end:-34100 | None:intergenic |
| TTCCTCCAATCCACAACGGA+CGG | 0.522694 | contig179end:-34378 | None:intergenic |
| AGTTTGACAATTAAGCAGTA+GGG | 0.525933 | contig179end:-35126 | None:intergenic |
| GATCATGGTGTCACCGCTGT+AGG | 0.528394 | contig179end:+34959 | MsG0080048038.01.T01:CDS |
| TCAGCTGTTGCAGCGACTGA+AGG | 0.530010 | contig179end:+34563 | MsG0080048038.01.T01:CDS |
| GAACTCATGGGGAACTGAGT+GGG | 0.530453 | contig179end:+35024 | MsG0080048038.01.T01:CDS |
| AAGGATACATTAGGATGCAA+AGG | 0.533659 | contig179end:+35053 | MsG0080048038.01.T01:CDS |
| TGTATGAGAGGCACGAGCAA+TGG | 0.535639 | contig179end:+34103 | MsG0080048038.01.T01:CDS |
| CTTGTTGATTGTGATACAAA+AGG | 0.539332 | contig179end:+34635 | MsG0080048038.01.T01:CDS |
| GTGGACCAAGGTTGTGAGGG+TGG | 0.540762 | contig179end:+34659 | MsG0080048038.01.T01:CDS |
| GTTACACCTGTGAAGAATCA+AGG | 0.542932 | contig179end:+34411 | MsG0080048038.01.T01:CDS |
| TCCTCCAATCCACAACGGAC+GGG | 0.547637 | contig179end:-34377 | None:intergenic |
| GTTGTGGATTGGAGGAAGAA+AGG | 0.549943 | contig179end:+34384 | MsG0080048038.01.T01:CDS |
| AAATTGAAAGTCAGATCCAC+TGG | 0.552701 | contig179end:-34894 | None:intergenic |
| GATGCCGCTGAAGGACTTTG+TGG | 0.554041 | contig179end:+35082 | MsG0080048038.01.T01:CDS |
| TGTCCCTGCCAACAATGAGC+AGG | 0.557594 | contig179end:+34823 | MsG0080048038.01.T01:CDS |
| GTAATGATGGAACCAAGTAT+TGG | 0.568388 | contig179end:+34993 | MsG0080048038.01.T01:CDS |
| TGATGGAACCAAGTATTGGT+TGG | 0.568906 | contig179end:+34997 | MsG0080048038.01.T01:CDS |
| CGGTTCATGTGGAACCGAGT+TGG | 0.572572 | contig179end:+34937 | MsG0080048038.01.T01:CDS |
| TTGGTTGGTTAAGAACTCAT+GGG | 0.574582 | contig179end:+35012 | MsG0080048038.01.T01:CDS |
| AGGGGAGTTGATGCCGCTGA+AGG | 0.575466 | contig179end:+35073 | MsG0080048038.01.T01:CDS |
| TCTGTTGCAATTGATGCCAG+TGG | 0.578268 | contig179end:+34878 | MsG0080048038.01.T01:CDS |
| TAAGACCACCCTCACAACCT+TGG | 0.579957 | contig179end:-34664 | None:intergenic |
| AAGTGCAATCCCGTCCGTTG+TGG | 0.579982 | contig179end:+34368 | MsG0080048038.01.T01:CDS |
| GGTGTCTTTACCGGTTCATG+TGG | 0.583724 | contig179end:+34926 | MsG0080048038.01.T01:CDS |
| TGTGGAACCGAGTTGGATCA+TGG | 0.584259 | contig179end:+34944 | MsG0080048038.01.T01:CDS |
| GCATCTAGAAATAAATTCAA+GGG | 0.590276 | contig179end:+34294 | MsG0080048038.01.T01:CDS |
| ATGCCTGCTCATTGTTGGCA+GGG | 0.601537 | contig179end:-34826 | None:intergenic |
| AACTCATGGGGAACTGAGTG+GGG | 0.603863 | contig179end:+35025 | MsG0080048038.01.T01:CDS |
| GGCAGGGACATCTTCATACC+CGG | 0.606676 | contig179end:-34810 | None:intergenic |
| ACCGCTGTAGGTTATGGTGT+CGG | 0.606963 | contig179end:+34971 | MsG0080048038.01.T01:CDS |
| TTCATGCAGCTACCATTACC+GGG | 0.608280 | contig179end:+34792 | MsG0080048038.01.T01:CDS |
| GAAGCCGCATACCCTTATCA+AGG | 0.613478 | contig179end:+34734 | MsG0080048038.01.T01:CDS |
| GGTGTGGACCAAGGTTGTGA+GGG | 0.614905 | contig179end:+34656 | MsG0080048038.01.T01:CDS |
| TAAGGATCCTCAAGAAAGGG+AGG | 0.633799 | contig179end:+34149 | MsG0080048038.01.T01:CDS |
| AGGTGTGGACCAAGGTTGTG+AGG | 0.640947 | contig179end:+34655 | MsG0080048038.01.T01:CDS |
| CTATGAACTCCTCATTGGTG+AGG | 0.645140 | contig179end:-34272 | None:intergenic |
| TGGTTGGTTAAGAACTCATG+GGG | 0.647156 | contig179end:+35013 | MsG0080048038.01.T01:CDS |
| AAGTCGACTCCATGTATGAG+AGG | 0.647740 | contig179end:+34091 | None:intergenic |
| AGAACTCATGGGGAACTGAG+TGG | 0.653772 | contig179end:+35023 | MsG0080048038.01.T01:CDS |
| TGATTGTGATACAAAAGGTG+TGG | 0.663878 | contig179end:+34640 | MsG0080048038.01.T01:CDS |
| ATTTGCAGACCTCACCAATG+AGG | 0.664553 | contig179end:+34263 | MsG0080048038.01.T01:CDS |
| AATGCCACAAAGTCCTTCAG+CGG | 0.670519 | contig179end:-35086 | None:intergenic |
| AACACCTTGATAAGGGTATG+CGG | 0.671998 | contig179end:-34738 | None:intergenic |
| GTGAAGAATCAAGGTCAATG+TGG | 0.680408 | contig179end:+34420 | MsG0080048038.01.T01:CDS |
| TTTCTTCCTCCAATCCACAA+CGG | 0.681262 | contig179end:-34382 | None:intergenic |
| GGTGACACCATGATCCAACT+CGG | 0.706899 | contig179end:-34951 | None:intergenic |
| ACCGACACCATAACCTACAG+CGG | 0.712402 | contig179end:-34972 | None:intergenic |
| GATACAAAAGGTGTGGACCA+AGG | 0.723079 | contig179end:+34647 | MsG0080048038.01.T01:CDS |
| GGATACATTAGGATGCAAAG+GGG | 0.764149 | contig179end:+35055 | MsG0080048038.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGTAAATTACAATTAATTA+TGG | - | contig179end:34513-34532 | None:intergenic | 10.0% |
| !! | AAATTCATTATTCAAAATCA+TGG | + | contig179end:34701-34720 | MsG0080048038.01.T01:CDS | 15.0% |
| !!! | AATTGTAATTTACTTTCTTT+TGG | + | contig179end:34518-34537 | MsG0080048038.01.T01:intron | 15.0% |
| ! | AATAACAAGCTATACAAGTT+AGG | + | contig179end:34231-34250 | MsG0080048038.01.T01:CDS | 25.0% |
| ! | AGCATCTAGAAATAAATTCA+AGG | + | contig179end:34293-34312 | MsG0080048038.01.T01:CDS | 25.0% |
| ! | GCATCTAGAAATAAATTCAA+GGG | + | contig179end:34294-34313 | MsG0080048038.01.T01:CDS | 25.0% |
| !! | ATTGAAGTTTTCAACAATGA+TGG | + | contig179end:34207-34226 | MsG0080048038.01.T01:CDS | 25.0% |
| !! | GACTTTCAATTTTACAAAAG+TGG | + | contig179end:34905-34924 | MsG0080048038.01.T01:CDS | 25.0% |
| !!! | AATTTACTTTCTTTTGGTGT+AGG | + | contig179end:34524-34543 | MsG0080048038.01.T01:intron | 25.0% |
| TCAATATGGCAAAGTCTATA+AGG | + | contig179end:34131-34150 | MsG0080048038.01.T01:CDS | 30.0% | |
| ! | CTTGTTGATTGTGATACAAA+AGG | + | contig179end:34635-34654 | MsG0080048038.01.T01:CDS | 30.0% |
| ! | GGAATTAATAAGTTGAGTAC+TGG | + | contig179end:34584-34603 | MsG0080048038.01.T01:CDS | 30.0% |
| AAATTGAAAGTCAGATCCAC+TGG | - | contig179end:34897-34916 | None:intergenic | 35.0% | |
| AAGGATACATTAGGATGCAA+AGG | + | contig179end:35053-35072 | MsG0080048038.01.T01:CDS | 35.0% | |
| AGGATACATTAGGATGCAAA+GGG | + | contig179end:35054-35073 | MsG0080048038.01.T01:CDS | 35.0% | |
| CTATAAGGATCCTCAAGAAA+GGG | + | contig179end:34146-34165 | MsG0080048038.01.T01:CDS | 35.0% | |
| GCATCAATTGCAACAGAAAT+AGG | - | contig179end:34875-34894 | None:intergenic | 35.0% | |
| GTTCTTAACCAACCAATACT+TGG | - | contig179end:35008-35027 | None:intergenic | 35.0% | |
| TACAAAAGTGGTGTCTTTAC+CGG | + | contig179end:34917-34936 | MsG0080048038.01.T01:CDS | 35.0% | |
| TCTATAAGGATCCTCAAGAA+AGG | + | contig179end:34145-34164 | MsG0080048038.01.T01:CDS | 35.0% | |
| TGATTGTGATACAAAAGGTG+TGG | + | contig179end:34640-34659 | MsG0080048038.01.T01:CDS | 35.0% | |
| TTCCATCAACACCTTGATAA+GGG | - | contig179end:34748-34767 | None:intergenic | 35.0% | |
| ! | ATTGGTTGGTTAAGAACTCA+TGG | + | contig179end:35011-35030 | MsG0080048038.01.T01:CDS | 35.0% |
| ! | GTAATGATGGAACCAAGTAT+TGG | + | contig179end:34993-35012 | MsG0080048038.01.T01:CDS | 35.0% |
| ! | TTGGTTGGTTAAGAACTCAT+GGG | + | contig179end:35012-35031 | MsG0080048038.01.T01:CDS | 35.0% |
| !! | AGTATTTGTTTTCGCGATTG+AGG | - | contig179end:34331-34350 | None:intergenic | 35.0% |
| !! | TAAGTTGAGTACTGGAAAGT+TGG | + | contig179end:34592-34611 | MsG0080048038.01.T01:CDS | 35.0% |
| !! | TTTTGGTGTAGGATGTTGTT+GGG | + | contig179end:34535-34554 | MsG0080048038.01.T01:intron | 35.0% |
| AGATGCTATGAACTCCTCAT+TGG | - | contig179end:34280-34299 | None:intergenic | 40.0% | |
| ATTCATGCAGCTACCATTAC+CGG | + | contig179end:34791-34810 | MsG0080048038.01.T01:CDS | 40.0% | |
| CATTGACCTTGATTCTTCAC+AGG | - | contig179end:34420-34439 | None:intergenic | 40.0% | |
| GAGCAATGGATGAGTCAATA+TGG | + | contig179end:34117-34136 | MsG0080048038.01.T01:CDS | 40.0% | |
| GGATACATTAGGATGCAAAG+GGG | + | contig179end:35055-35074 | MsG0080048038.01.T01:CDS | 40.0% | |
| GTGAAGAATCAAGGTCAATG+TGG | + | contig179end:34420-34439 | MsG0080048038.01.T01:CDS | 40.0% | |
| GTTACACCTGTGAAGAATCA+AGG | + | contig179end:34411-34430 | MsG0080048038.01.T01:CDS | 40.0% | |
| GTTCCATCAACACCTTGATA+AGG | - | contig179end:34749-34768 | None:intergenic | 40.0% | |
| TTTCTTCCTCCAATCCACAA+CGG | - | contig179end:34385-34404 | None:intergenic | 40.0% | |
| ! | AACACCTTGATAAGGGTATG+CGG | - | contig179end:34741-34760 | None:intergenic | 40.0% |
| ! | TGGTTGGTTAAGAACTCATG+GGG | + | contig179end:35013-35032 | MsG0080048038.01.T01:CDS | 40.0% |
| !! | CTTTTGGTGTAGGATGTTGT+TGG | + | contig179end:34534-34553 | MsG0080048038.01.T01:intron | 40.0% |
| !! | TACCCTTATCAAGGTGTTGA+TGG | + | contig179end:34743-34762 | MsG0080048038.01.T01:CDS | 40.0% |
| !! | TGATGGAACCAAGTATTGGT+TGG | + | contig179end:34997-35016 | MsG0080048038.01.T01:CDS | 40.0% |
| AATGCCACAAAGTCCTTCAG+CGG | - | contig179end:35089-35108 | None:intergenic | 45.0% | |
| ATTTGCAGACCTCACCAATG+AGG | + | contig179end:34263-34282 | MsG0080048038.01.T01:CDS | 45.0% | |
| CTATGAACTCCTCATTGGTG+AGG | - | contig179end:34275-34294 | None:intergenic | 45.0% | |
| GACATCTTCATACCCGGTAA+TGG | - | contig179end:34807-34826 | None:intergenic | 45.0% | |
| GGGGTGAAGAAGGATACATT+AGG | + | contig179end:35044-35063 | MsG0080048038.01.T01:CDS | 45.0% | |
| TAAGGATCCTCAAGAAAGGG+AGG | + | contig179end:34149-34168 | MsG0080048038.01.T01:CDS | 45.0% | |
| TCTGTTGCAATTGATGCCAG+TGG | + | contig179end:34878-34897 | MsG0080048038.01.T01:CDS | 45.0% | |
| TTCATGCAGCTACCATTACC+GGG | + | contig179end:34792-34811 | MsG0080048038.01.T01:CDS | 45.0% | |
| TTGCAATGCCTGCTCATTGT+TGG | - | contig179end:34834-34853 | None:intergenic | 45.0% | |
| ! | GATACAAAAGGTGTGGACCA+AGG | + | contig179end:34647-34666 | MsG0080048038.01.T01:CDS | 45.0% |
| ! | GGTAATGGTAGCTGCATGAA+TGG | - | contig179end:34792-34811 | None:intergenic | 45.0% |
| !! | GGTTATGGTGTCGGTAATGA+TGG | + | contig179end:34980-34999 | MsG0080048038.01.T01:CDS | 45.0% |
| !! | GTTGTGGATTGGAGGAAGAA+AGG | + | contig179end:34384-34403 | MsG0080048038.01.T01:CDS | 45.0% |
| AACTCATGGGGAACTGAGTG+GGG | + | contig179end:35025-35044 | MsG0080048038.01.T01:CDS | 50.0% | |
| AATGCCTGCTCATTGTTGGC+AGG | - | contig179end:34830-34849 | None:intergenic | 50.0% | |
| ACCGACACCATAACCTACAG+CGG | - | contig179end:34975-34994 | None:intergenic | 50.0% | |
| ACCGCTGTAGGTTATGGTGT+CGG | + | contig179end:34971-34990 | MsG0080048038.01.T01:CDS | 50.0% | |
| AGAACTCATGGGGAACTGAG+TGG | + | contig179end:35023-35042 | MsG0080048038.01.T01:CDS | 50.0% | |
| AGACGTTCCTCCCTTTCTTG+AGG | - | contig179end:34159-34178 | None:intergenic | 50.0% | |
| ATGCCTGCTCATTGTTGGCA+GGG | - | contig179end:34829-34848 | None:intergenic | 50.0% | |
| CAACTCGGTTCCACATGAAC+CGG | - | contig179end:34939-34958 | None:intergenic | 50.0% | |
| GAACTCATGGGGAACTGAGT+GGG | + | contig179end:35024-35043 | MsG0080048038.01.T01:CDS | 50.0% | |
| GAAGCCGCATACCCTTATCA+AGG | + | contig179end:34734-34753 | MsG0080048038.01.T01:CDS | 50.0% | |
| GGTGACACCATGATCCAACT+CGG | - | contig179end:34954-34973 | None:intergenic | 50.0% | |
| TAAGACCACCCTCACAACCT+TGG | - | contig179end:34667-34686 | None:intergenic | 50.0% | |
| TTCCTCCAATCCACAACGGA+CGG | - | contig179end:34381-34400 | None:intergenic | 50.0% | |
| ! | AGGTTGTGAGGGTGGTCTTA+TGG | + | contig179end:34667-34686 | MsG0080048038.01.T01:CDS | 50.0% |
| ! | TGTATGAGAGGCACGAGCAA+TGG | + | contig179end:34103-34122 | MsG0080048038.01.T01:CDS | 50.0% |
| ! | TGTGGAACCGAGTTGGATCA+TGG | + | contig179end:34944-34963 | MsG0080048038.01.T01:CDS | 50.0% |
| !! | GGTGTCTTTACCGGTTCATG+TGG | + | contig179end:34926-34945 | MsG0080048038.01.T01:CDS | 50.0% |
| AAGTGCAATCCCGTCCGTTG+TGG | + | contig179end:34368-34387 | MsG0080048038.01.T01:CDS | 55.0% | |
| AGGTGTGGACCAAGGTTGTG+AGG | + | contig179end:34655-34674 | MsG0080048038.01.T01:CDS | 55.0% | |
| CAATCCCGTCCGTTGTGGAT+TGG | + | contig179end:34373-34392 | MsG0080048038.01.T01:CDS | 55.0% | |
| CGGTTCATGTGGAACCGAGT+TGG | + | contig179end:34937-34956 | MsG0080048038.01.T01:CDS | 55.0% | |
| GATGCCGCTGAAGGACTTTG+TGG | + | contig179end:35082-35101 | MsG0080048038.01.T01:CDS | 55.0% | |
| GGAACTGAGTGGGGTGAAGA+AGG | + | contig179end:35034-35053 | MsG0080048038.01.T01:CDS | 55.0% | |
| GGCAGGGACATCTTCATACC+CGG | - | contig179end:34813-34832 | None:intergenic | 55.0% | |
| GGTGTGGACCAAGGTTGTGA+GGG | + | contig179end:34656-34675 | MsG0080048038.01.T01:CDS | 55.0% | |
| TCAGCTGTTGCAGCGACTGA+AGG | + | contig179end:34563-34582 | MsG0080048038.01.T01:CDS | 55.0% | |
| TCCTCCAATCCACAACGGAC+GGG | - | contig179end:34380-34399 | None:intergenic | 55.0% | |
| TGTCCCTGCCAACAATGAGC+AGG | + | contig179end:34823-34842 | MsG0080048038.01.T01:CDS | 55.0% | |
| ! | GGTGTCACCGCTGTAGGTTA+TGG | + | contig179end:34965-34984 | MsG0080048038.01.T01:CDS | 55.0% |
| !! | GATCATGGTGTCACCGCTGT+AGG | + | contig179end:34959-34978 | MsG0080048038.01.T01:CDS | 55.0% |
| GTGGACCAAGGTTGTGAGGG+TGG | + | contig179end:34659-34678 | MsG0080048038.01.T01:CDS | 60.0% | |
| !! | AGGGGAGTTGATGCCGCTGA+AGG | + | contig179end:35073-35092 | MsG0080048038.01.T01:CDS | 60.0% |
| !! | TCCCGTCCGTTGTGGATTGG+AGG | + | contig179end:34376-34395 | MsG0080048038.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig179end | gene | 34102 | 35138 | 34102 | ID=MsG0080048038.01;Name=MsG0080048038.01 |
| contig179end | mRNA | 34102 | 35138 | 34102 | ID=MsG0080048038.01.T01;Parent=MsG0080048038.01;Name=MsG0080048038.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|310 |
| contig179end | exon | 34102 | 34441 | 34102 | ID=MsG0080048038.01.T01:exon:7864;Parent=MsG0080048038.01.T01 |
| contig179end | exon | 34546 | 35138 | 34546 | ID=MsG0080048038.01.T01:exon:7863;Parent=MsG0080048038.01.T01 |
| contig179end | CDS | 34102 | 34441 | 34102 | ID=MsG0080048038.01.T01:cds;Parent=MsG0080048038.01.T01 |
| contig179end | CDS | 34546 | 35138 | 34546 | ID=MsG0080048038.01.T01:cds;Parent=MsG0080048038.01.T01 |
| Gene Sequence |
| Protein sequence |
