Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046427.01.T01 | XP_003609015.1 | 97.101 | 345 | 10 | 0 | 1 | 345 | 1 | 345 | 0 | 702 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046427.01.T01 | A2XQE8 | 59.483 | 348 | 129 | 6 | 1 | 345 | 1 | 339 | 1.91E-149 | 427 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046427.01.T01 | G7JPY3 | 97.101 | 345 | 10 | 0 | 1 | 345 | 1 | 345 | 0.0 | 702 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048038.01 | MsG0880046427.01 | 0.951396 | 2.231183e-109 | 5.074671e-104 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046427.01.T01 | MTR_4g107930 | 97.101 | 345 | 10 | 0 | 1 | 345 | 1 | 345 | 0.0 | 702 |
| MsG0880046427.01.T01 | MTR_4g079770 | 89.306 | 346 | 35 | 2 | 1 | 345 | 1 | 345 | 0.0 | 642 |
| MsG0880046427.01.T01 | MTR_5g022560 | 87.826 | 345 | 41 | 1 | 1 | 345 | 1 | 344 | 0.0 | 635 |
| MsG0880046427.01.T01 | MTR_4g047610 | 87.246 | 345 | 42 | 2 | 1 | 345 | 1 | 343 | 0.0 | 613 |
| MsG0880046427.01.T01 | MTR_4g079470 | 91.049 | 324 | 27 | 2 | 23 | 345 | 23 | 345 | 0.0 | 612 |
| MsG0880046427.01.T01 | MTR_4g079800 | 87.654 | 324 | 38 | 2 | 23 | 345 | 23 | 345 | 0.0 | 591 |
| MsG0880046427.01.T01 | MTR_2g075830 | 75.652 | 345 | 80 | 3 | 1 | 345 | 1 | 341 | 0.0 | 524 |
| MsG0880046427.01.T01 | MTR_4g080700 | 65.421 | 321 | 105 | 5 | 26 | 345 | 25 | 340 | 1.97e-152 | 432 |
| MsG0880046427.01.T01 | MTR_4g080730 | 65.421 | 321 | 105 | 5 | 26 | 345 | 25 | 340 | 9.92e-152 | 430 |
| MsG0880046427.01.T01 | MTR_4g080360 | 53.314 | 347 | 155 | 5 | 1 | 345 | 1 | 342 | 1.03e-131 | 379 |
| MsG0880046427.01.T01 | MTR_2g083930 | 52.754 | 345 | 149 | 5 | 4 | 343 | 7 | 342 | 1.24e-128 | 372 |
| MsG0880046427.01.T01 | MTR_4g104370 | 55.043 | 347 | 147 | 7 | 1 | 343 | 1 | 342 | 2.20e-128 | 372 |
| MsG0880046427.01.T01 | MTR_4g079440 | 53.043 | 345 | 143 | 6 | 11 | 343 | 6 | 343 | 3.45e-125 | 363 |
| MsG0880046427.01.T01 | MTR_3g075390 | 51.594 | 345 | 143 | 6 | 15 | 343 | 7 | 343 | 4.60e-124 | 361 |
| MsG0880046427.01.T01 | MTR_7g078600 | 57.419 | 310 | 121 | 6 | 35 | 343 | 37 | 336 | 4.80e-121 | 352 |
| MsG0880046427.01.T01 | MTR_2g090890 | 50.898 | 334 | 157 | 4 | 11 | 344 | 10 | 336 | 8.13e-119 | 347 |
| MsG0880046427.01.T01 | MTR_3g116080 | 51.622 | 339 | 151 | 7 | 11 | 344 | 17 | 347 | 1.24e-117 | 344 |
| MsG0880046427.01.T01 | MTR_7g079230 | 55.016 | 309 | 131 | 5 | 35 | 343 | 34 | 334 | 2.58e-117 | 343 |
| MsG0880046427.01.T01 | MTR_1g107550 | 55.063 | 316 | 131 | 7 | 34 | 343 | 44 | 354 | 5.63e-116 | 344 |
| MsG0880046427.01.T01 | MTR_4g094918 | 49.711 | 346 | 156 | 9 | 10 | 344 | 3 | 341 | 1.04e-113 | 334 |
| MsG0880046427.01.T01 | MTR_1g018840 | 52.336 | 321 | 134 | 7 | 32 | 343 | 50 | 360 | 6.32e-110 | 328 |
| MsG0880046427.01.T01 | MTR_1g018840 | 52.336 | 321 | 134 | 7 | 32 | 343 | 50 | 360 | 1.03e-109 | 328 |
| MsG0880046427.01.T01 | MTR_3g102220 | 52.063 | 315 | 133 | 7 | 40 | 343 | 53 | 360 | 7.06e-109 | 326 |
| MsG0880046427.01.T01 | MTR_3g112430 | 50.482 | 311 | 141 | 5 | 41 | 344 | 37 | 341 | 1.02e-101 | 307 |
| MsG0880046427.01.T01 | MTR_2g015850 | 53.793 | 290 | 127 | 5 | 56 | 343 | 78 | 362 | 4.05e-98 | 295 |
| MsG0880046427.01.T01 | MTR_2g016180 | 45.376 | 346 | 168 | 10 | 12 | 344 | 18 | 355 | 1.59e-90 | 280 |
| MsG0880046427.01.T01 | MTR_2g016270 | 45.190 | 343 | 158 | 10 | 12 | 344 | 18 | 340 | 1.02e-88 | 275 |
| MsG0880046427.01.T01 | MTR_2g067900 | 45.210 | 334 | 157 | 13 | 23 | 342 | 2 | 323 | 1.84e-79 | 246 |
| MsG0880046427.01.T01 | MTR_2g064475 | 41.714 | 350 | 147 | 17 | 39 | 344 | 15 | 351 | 7.62e-73 | 230 |
| MsG0880046427.01.T01 | MTR_2g067560 | 45.652 | 322 | 142 | 14 | 39 | 339 | 15 | 324 | 1.51e-72 | 241 |
| MsG0880046427.01.T01 | MTR_4g125300 | 42.071 | 309 | 160 | 10 | 42 | 344 | 53 | 348 | 1.65e-68 | 218 |
| MsG0880046427.01.T01 | MTR_7g078630 | 45.035 | 282 | 113 | 5 | 34 | 304 | 700 | 950 | 2.82e-68 | 231 |
| MsG0880046427.01.T01 | MTR_2g015920 | 37.677 | 353 | 194 | 12 | 11 | 344 | 13 | 358 | 2.87e-68 | 219 |
| MsG0880046427.01.T01 | MTR_2g066980 | 36.798 | 356 | 164 | 13 | 10 | 344 | 3 | 318 | 2.79e-67 | 214 |
| MsG0880046427.01.T01 | MTR_4g065077 | 38.509 | 322 | 177 | 10 | 34 | 344 | 45 | 356 | 1.32e-65 | 211 |
| MsG0880046427.01.T01 | MTR_2g015930 | 39.623 | 318 | 172 | 11 | 38 | 344 | 50 | 358 | 1.03e-64 | 209 |
| MsG0880046427.01.T01 | MTR_4g071060 | 39.871 | 311 | 161 | 9 | 46 | 341 | 54 | 353 | 7.00e-63 | 204 |
| MsG0880046427.01.T01 | MTR_1g023210 | 39.048 | 315 | 151 | 11 | 45 | 336 | 56 | 352 | 5.18e-62 | 202 |
| MsG0880046427.01.T01 | MTR_1g023810 | 44.635 | 233 | 120 | 6 | 113 | 342 | 35 | 261 | 6.80e-62 | 199 |
| MsG0880046427.01.T01 | MTR_1g094135 | 36.236 | 356 | 192 | 10 | 9 | 341 | 20 | 363 | 4.35e-61 | 200 |
| MsG0880046427.01.T01 | MTR_8g086470 | 36.875 | 320 | 169 | 9 | 34 | 344 | 61 | 356 | 8.25e-60 | 196 |
| MsG0880046427.01.T01 | MTR_4g072790 | 34.202 | 307 | 156 | 10 | 42 | 341 | 50 | 317 | 1.39e-43 | 153 |
| MsG0880046427.01.T01 | MTR_7g078580 | 64.286 | 98 | 34 | 1 | 246 | 343 | 4 | 100 | 1.22e-41 | 142 |
| MsG0880046427.01.T01 | MTR_2g015840 | 49.664 | 149 | 72 | 2 | 16 | 162 | 9 | 156 | 3.16e-41 | 145 |
| MsG0880046427.01.T01 | MTR_2g015840 | 56.250 | 112 | 48 | 1 | 233 | 344 | 161 | 271 | 1.32e-36 | 133 |
| MsG0880046427.01.T01 | MTR_7g079130 | 70.000 | 90 | 27 | 0 | 254 | 343 | 7 | 96 | 1.12e-40 | 138 |
| MsG0880046427.01.T01 | MTR_4g072340 | 33.657 | 309 | 159 | 10 | 42 | 341 | 50 | 321 | 5.36e-39 | 141 |
| MsG0880046427.01.T01 | MTR_4g073660 | 33.657 | 309 | 159 | 10 | 42 | 341 | 49 | 320 | 5.76e-39 | 141 |
| MsG0880046427.01.T01 | MTR_1g025450 | 39.891 | 183 | 83 | 8 | 121 | 297 | 47 | 208 | 1.60e-31 | 124 |
| MsG0880046427.01.T01 | MTR_7g078310 | 43.571 | 140 | 70 | 2 | 83 | 220 | 115 | 247 | 1.28e-28 | 112 |
| MsG0880046427.01.T01 | MTR_2g016380 | 47.107 | 121 | 57 | 4 | 228 | 344 | 18 | 135 | 2.28e-27 | 108 |
| MsG0880046427.01.T01 | MTR_7g111060 | 26.071 | 280 | 172 | 8 | 79 | 336 | 64 | 330 | 1.18e-22 | 97.8 |
| MsG0880046427.01.T01 | MTR_7g079260 | 59.722 | 72 | 13 | 1 | 270 | 341 | 7 | 62 | 3.26e-22 | 90.5 |
| MsG0880046427.01.T01 | MTR_7g111050 | 30.085 | 236 | 133 | 9 | 133 | 344 | 110 | 337 | 8.34e-22 | 95.1 |
| MsG0880046427.01.T01 | MTR_3g029560 | 27.200 | 250 | 150 | 8 | 99 | 344 | 16 | 237 | 1.08e-20 | 90.1 |
| MsG0880046427.01.T01 | MTR_8g087510 | 41.985 | 131 | 69 | 5 | 168 | 296 | 23 | 148 | 1.33e-20 | 88.2 |
| MsG0880046427.01.T01 | MTR_2g047960 | 49.412 | 85 | 42 | 1 | 136 | 220 | 7 | 90 | 5.66e-19 | 81.3 |
| MsG0880046427.01.T01 | MTR_3g082370 | 26.442 | 208 | 131 | 7 | 132 | 323 | 5 | 206 | 2.49e-16 | 77.8 |
| MsG0880046427.01.T01 | MTR_7g111050 | 28.061 | 196 | 115 | 7 | 133 | 306 | 105 | 296 | 1.55e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046427.01.T01 | AT5G45890 | 54.701 | 351 | 146 | 6 | 1 | 344 | 1 | 345 | 2.28e-134 | 386 |
| MsG0880046427.01.T01 | AT5G50260 | 53.757 | 346 | 134 | 6 | 16 | 343 | 4 | 341 | 7.09e-128 | 370 |
| MsG0880046427.01.T01 | AT3G48340 | 53.582 | 349 | 136 | 9 | 11 | 343 | 4 | 342 | 3.24e-123 | 358 |
| MsG0880046427.01.T01 | AT2G34080 | 51.462 | 342 | 155 | 6 | 11 | 345 | 8 | 345 | 1.11e-121 | 354 |
| MsG0880046427.01.T01 | AT4G35350 | 49.858 | 351 | 160 | 7 | 3 | 344 | 9 | 352 | 8.35e-119 | 347 |
| MsG0880046427.01.T01 | AT2G27420 | 49.425 | 348 | 159 | 6 | 12 | 345 | 4 | 348 | 5.75e-116 | 340 |
| MsG0880046427.01.T01 | AT3G49340 | 50.000 | 342 | 146 | 7 | 21 | 345 | 8 | 341 | 8.85e-116 | 339 |
| MsG0880046427.01.T01 | AT3G48350 | 50.720 | 347 | 154 | 7 | 11 | 344 | 1 | 343 | 2.15e-114 | 336 |
| MsG0880046427.01.T01 | AT3G19390 | 49.853 | 339 | 163 | 5 | 7 | 344 | 13 | 345 | 6.95e-114 | 338 |
| MsG0880046427.01.T01 | AT5G43060 | 52.761 | 326 | 140 | 7 | 25 | 343 | 34 | 352 | 3.22e-113 | 337 |
| MsG0880046427.01.T01 | AT1G20850 | 52.077 | 313 | 144 | 4 | 34 | 344 | 45 | 353 | 2.58e-111 | 328 |
| MsG0880046427.01.T01 | AT1G29090 | 47.701 | 348 | 167 | 8 | 7 | 345 | 14 | 355 | 2.81e-111 | 328 |
| MsG0880046427.01.T01 | AT1G06260 | 51.176 | 340 | 153 | 6 | 10 | 343 | 9 | 341 | 2.27e-110 | 325 |
| MsG0880046427.01.T01 | AT4G36880 | 51.553 | 322 | 141 | 7 | 32 | 343 | 44 | 360 | 6.88e-110 | 325 |
| MsG0880046427.01.T01 | AT3G19400 | 49.068 | 322 | 157 | 5 | 25 | 344 | 32 | 348 | 9.50e-109 | 322 |
| MsG0880046427.01.T01 | AT1G29080 | 47.399 | 346 | 169 | 8 | 8 | 345 | 6 | 346 | 6.31e-108 | 319 |
| MsG0880046427.01.T01 | AT1G47128 | 50.809 | 309 | 140 | 7 | 40 | 343 | 50 | 351 | 1.16e-106 | 320 |
| MsG0880046427.01.T01 | AT1G09850 | 49.355 | 310 | 154 | 3 | 34 | 343 | 26 | 332 | 1.43e-101 | 306 |
| MsG0880046427.01.T01 | AT4G23520 | 44.857 | 350 | 168 | 10 | 11 | 343 | 7 | 348 | 1.77e-99 | 298 |
| MsG0880046427.01.T01 | AT4G11310 | 47.896 | 309 | 148 | 6 | 41 | 343 | 50 | 351 | 2.16e-98 | 296 |
| MsG0880046427.01.T01 | AT4G11320 | 47.249 | 309 | 150 | 6 | 41 | 343 | 57 | 358 | 1.50e-97 | 294 |
| MsG0880046427.01.T01 | AT4G11320 | 47.249 | 309 | 150 | 6 | 41 | 343 | 57 | 358 | 1.50e-97 | 294 |
| MsG0880046427.01.T01 | AT1G29110 | 42.330 | 352 | 176 | 9 | 1 | 344 | 1 | 333 | 2.40e-89 | 271 |
| MsG0880046427.01.T01 | AT3G43960 | 45.912 | 318 | 158 | 9 | 32 | 343 | 36 | 345 | 6.20e-86 | 264 |
| MsG0880046427.01.T01 | AT4G35350 | 46.667 | 285 | 136 | 7 | 3 | 278 | 9 | 286 | 1.11e-83 | 255 |
| MsG0880046427.01.T01 | AT3G19400 | 47.266 | 256 | 129 | 4 | 25 | 278 | 32 | 283 | 1.50e-75 | 234 |
| MsG0880046427.01.T01 | AT3G48350 | 46.619 | 281 | 133 | 7 | 11 | 278 | 1 | 277 | 4.16e-75 | 234 |
| MsG0880046427.01.T01 | AT5G60360 | 43.042 | 309 | 157 | 11 | 42 | 344 | 61 | 356 | 5.24e-71 | 225 |
| MsG0880046427.01.T01 | AT3G45310 | 40.673 | 327 | 171 | 12 | 24 | 344 | 47 | 356 | 1.69e-68 | 219 |
| MsG0880046427.01.T01 | AT5G60360 | 42.718 | 309 | 157 | 12 | 42 | 344 | 61 | 355 | 1.70e-68 | 219 |
| MsG0880046427.01.T01 | AT5G60360 | 42.333 | 300 | 154 | 11 | 42 | 335 | 61 | 347 | 1.06e-66 | 214 |
| MsG0880046427.01.T01 | AT3G45310 | 40.122 | 329 | 173 | 13 | 23 | 345 | 46 | 356 | 5.39e-66 | 212 |
| MsG0880046427.01.T01 | AT4G39090 | 38.298 | 329 | 170 | 11 | 32 | 341 | 43 | 357 | 1.06e-62 | 204 |
| MsG0880046427.01.T01 | AT2G21430 | 35.028 | 354 | 197 | 11 | 6 | 336 | 6 | 349 | 1.97e-62 | 203 |
| MsG0880046427.01.T01 | AT3G54940 | 35.833 | 360 | 182 | 13 | 11 | 336 | 10 | 354 | 8.35e-59 | 194 |
| MsG0880046427.01.T01 | AT4G16190 | 38.095 | 315 | 166 | 10 | 45 | 341 | 60 | 363 | 8.41e-57 | 189 |
| MsG0880046427.01.T01 | AT3G54940 | 35.165 | 273 | 148 | 8 | 11 | 260 | 10 | 276 | 2.16e-43 | 151 |
| MsG0880046427.01.T01 | AT1G02305 | 30.222 | 225 | 129 | 7 | 133 | 336 | 116 | 333 | 6.05e-24 | 101 |
| MsG0880046427.01.T01 | AT4G01610 | 27.273 | 286 | 176 | 9 | 76 | 336 | 52 | 330 | 2.04e-22 | 97.1 |
| MsG0880046427.01.T01 | AT4G01610 | 26.923 | 286 | 177 | 9 | 76 | 336 | 52 | 330 | 3.88e-21 | 93.6 |
| MsG0880046427.01.T01 | AT1G02300 | 29.384 | 211 | 123 | 6 | 146 | 336 | 146 | 350 | 8.38e-20 | 89.7 |
| MsG0880046427.01.T01 | AT2G22160 | 39.130 | 92 | 54 | 1 | 55 | 146 | 9 | 98 | 3.92e-14 | 68.2 |
Find 88 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATCAATTGCAACAGAAAT+AGG | 0.303591 | 8:+73263282 | None:intergenic |
| TCAATATGGCAAAGTCTATA+AGG | 0.330619 | 8:-73264023 | MsG0880046427.01.T01:CDS |
| TACAAAAGTGGTGTCTTTAC+CGG | 0.332301 | 8:-73263237 | MsG0880046427.01.T01:CDS |
| AGGTTGTGAGGGTGGTCTTA+TGG | 0.350865 | 8:-73263487 | MsG0880046427.01.T01:CDS |
| AAATTCATTATTCAAAATCA+TGG | 0.369502 | 8:-73263453 | MsG0880046427.01.T01:CDS |
| TTGCTCGAATTACAAAGTTT+AGG | 0.373232 | 8:+73262991 | None:intergenic |
| TTGCAATGCCTGCTCATTGT+TGG | 0.384601 | 8:+73263323 | None:intergenic |
| ATTCATGCAGCTACCATTAC+CGG | 0.385380 | 8:-73263363 | MsG0880046427.01.T01:CDS |
| TACCCTTATCAAGGTGTTGA+TGG | 0.427747 | 8:-73263411 | MsG0880046427.01.T01:CDS |
| AATAACAAGCTATACAAGTT+AGG | 0.429513 | 8:-73263923 | MsG0880046427.01.T01:CDS |
| GGAATTAATAAGTTGAGTAC+TGG | 0.441256 | 8:-73263570 | MsG0880046427.01.T01:CDS |
| GGAACTGAGTGGGGTGAAGA+AGG | 0.443728 | 8:-73263120 | MsG0880046427.01.T01:CDS |
| GAGCAATGGATGAGTCAATA+TGG | 0.445016 | 8:-73264037 | MsG0880046427.01.T01:CDS |
| TTCCATCAACACCTTGATAA+GGG | 0.450919 | 8:+73263409 | None:intergenic |
| AATGCCTGCTCATTGTTGGC+AGG | 0.451756 | 8:+73263327 | None:intergenic |
| GTTCCATCAACACCTTGATA+AGG | 0.464383 | 8:+73263408 | None:intergenic |
| CAATCCCGTCCGTTGTGGAT+TGG | 0.469084 | 8:-73263781 | MsG0880046427.01.T01:CDS |
| GTTCTTAACCAACCAATACT+TGG | 0.469107 | 8:+73263149 | None:intergenic |
| AAGTTTGACAATTAAGCAGT+AGG | 0.473215 | 8:+73263027 | None:intergenic |
| TAAGTTGAGTACTGGAAAGT+TGG | 0.474031 | 8:-73263562 | MsG0880046427.01.T01:CDS |
| GGTAATGGTAGCTGCATGAA+TGG | 0.481667 | 8:+73263365 | None:intergenic |
| AGCATCTAGAAATAAATTCA+AGG | 0.484430 | 8:-73263861 | MsG0880046427.01.T01:CDS |
| AGATGCTATGAACTCCTCAT+TGG | 0.488280 | 8:+73263877 | None:intergenic |
| GTGATTGACTACATTCATCA+TGG | 0.493788 | 8:-73262964 | MsG0880046427.01.T01:three_prime_UTR |
| CATTGACCTTGATTCTTCAC+AGG | 0.495833 | 8:+73263737 | None:intergenic |
| ATTGGTTGGTTAAGAACTCA+TGG | 0.497126 | 8:-73263143 | MsG0880046427.01.T01:CDS |
| CAACTCGGTTCCACATGAAC+CGG | 0.497716 | 8:+73263218 | None:intergenic |
| GGGGTGAAGAAGGATACATT+AGG | 0.500551 | 8:-73263110 | MsG0880046427.01.T01:CDS |
| GGTGTCACCGCTGTAGGTTA+TGG | 0.501955 | 8:-73263189 | MsG0880046427.01.T01:CDS |
| TCTATAAGGATCCTCAAGAA+AGG | 0.505398 | 8:-73264009 | MsG0880046427.01.T01:CDS |
| TCCCGTCCGTTGTGGATTGG+AGG | 0.514340 | 8:-73263778 | MsG0880046427.01.T01:CDS |
| GGTTATGGTGTCGGTAATGA+TGG | 0.515702 | 8:-73263174 | MsG0880046427.01.T01:CDS |
| CTATAAGGATCCTCAAGAAA+GGG | 0.516295 | 8:-73264008 | MsG0880046427.01.T01:CDS |
| AGACGTTCCTCCCTTTCTTG+AGG | 0.517763 | 8:+73263998 | None:intergenic |
| GACATCTTCATACCCGGTAA+TGG | 0.518079 | 8:+73263350 | None:intergenic |
| TTGCTCGTGCCTCTCATACA+TGG | 0.519986 | 8:+73264054 | None:intergenic |
| TTCCTCCAATCCACAACGGA+CGG | 0.522694 | 8:+73263776 | None:intergenic |
| AGTTTGACAATTAAGCAGTA+GGG | 0.525933 | 8:+73263028 | None:intergenic |
| GATCATGGTGTCACCGCTGT+AGG | 0.528394 | 8:-73263195 | MsG0880046427.01.T01:CDS |
| TCAGCTGTTGCAGCGACTGA+AGG | 0.530010 | 8:-73263591 | MsG0880046427.01.T01:CDS |
| GAACTCATGGGGAACTGAGT+GGG | 0.530453 | 8:-73263130 | MsG0880046427.01.T01:CDS |
| TGTATGAGAGGCACGAGCAA+TGG | 0.535639 | 8:-73264051 | MsG0880046427.01.T01:CDS |
| TCTCATACATGGAGTCGACT+TGG | 0.538645 | 8:+73264065 | None:intergenic |
| CTTGTTGATTGTGATACAAA+AGG | 0.539332 | 8:-73263519 | MsG0880046427.01.T01:CDS |
| GTGGACCAAGGTTGTGAGGG+TGG | 0.540762 | 8:-73263495 | MsG0880046427.01.T01:CDS |
| AAGGATACATTAGGATGCGA+AGG | 0.541422 | 8:-73263101 | MsG0880046427.01.T01:CDS |
| GTTACACCTGTGAAGAATCA+AGG | 0.542932 | 8:-73263743 | MsG0880046427.01.T01:CDS |
| TCCTCCAATCCACAACGGAC+GGG | 0.547637 | 8:+73263777 | None:intergenic |
| GTTGTGGATTGGAGGAAGAA+AGG | 0.549943 | 8:-73263770 | MsG0880046427.01.T01:CDS |
| AAATTGAAAGTCAGATCCAC+TGG | 0.552701 | 8:+73263260 | None:intergenic |
| GATGCCGCTGAAGGACTTTG+TGG | 0.554041 | 8:-73263072 | MsG0880046427.01.T01:CDS |
| AGGGGAGTTGATGCCGCTGA+AGG | 0.554645 | 8:-73263081 | MsG0880046427.01.T01:CDS |
| TGTCCCTGCCAACAATGAGC+AGG | 0.557594 | 8:-73263331 | MsG0880046427.01.T01:CDS |
| TCACACTTCATTACCTACAA+TGG | 0.565451 | 8:-73264176 | MsG0880046427.01.T01:exon |
| GTAATGATGGAACCAAGTAT+TGG | 0.568388 | 8:-73263161 | MsG0880046427.01.T01:CDS |
| TGATGGAACCAAGTATTGGT+TGG | 0.568906 | 8:-73263157 | MsG0880046427.01.T01:CDS |
| CGGTTCATGTGGAACCGAGT+TGG | 0.572572 | 8:-73263217 | MsG0880046427.01.T01:CDS |
| TTGGTTGGTTAAGAACTCAT+GGG | 0.574582 | 8:-73263142 | MsG0880046427.01.T01:CDS |
| TCTGTTGCAATTGATGCCAG+TGG | 0.578268 | 8:-73263276 | MsG0880046427.01.T01:CDS |
| TAAGACCACCCTCACAACCT+TGG | 0.579957 | 8:+73263490 | None:intergenic |
| AAGTGCAATCCCGTCCGTTG+TGG | 0.579982 | 8:-73263786 | MsG0880046427.01.T01:CDS |
| GGTGTCTTTACCGGTTCATG+TGG | 0.583724 | 8:-73263228 | MsG0880046427.01.T01:CDS |
| TGTGGAACCGAGTTGGATCA+TGG | 0.584259 | 8:-73263210 | MsG0880046427.01.T01:CDS |
| GCATCTAGAAATAAATTCAA+GGG | 0.590276 | 8:-73263860 | MsG0880046427.01.T01:CDS |
| ATGCCTGCTCATTGTTGGCA+GGG | 0.601537 | 8:+73263328 | None:intergenic |
| AACTCATGGGGAACTGAGTG+GGG | 0.603863 | 8:-73263129 | MsG0880046427.01.T01:CDS |
| AGGATACATTAGGATGCGAA+GGG | 0.605030 | 8:-73263100 | MsG0880046427.01.T01:CDS |
| GGCAGGGACATCTTCATACC+CGG | 0.606676 | 8:+73263344 | None:intergenic |
| ACCGCTGTAGGTTATGGTGT+CGG | 0.606963 | 8:-73263183 | MsG0880046427.01.T01:CDS |
| TTCATGCAGCTACCATTACC+GGG | 0.608280 | 8:-73263362 | MsG0880046427.01.T01:CDS |
| GAAGCCGCATACCCTTATCA+AGG | 0.613478 | 8:-73263420 | MsG0880046427.01.T01:CDS |
| GGTGTGGACCAAGGTTGTGA+GGG | 0.614905 | 8:-73263498 | MsG0880046427.01.T01:CDS |
| TAAGGATCCTCAAGAAAGGG+AGG | 0.633799 | 8:-73264005 | MsG0880046427.01.T01:CDS |
| AGGTGTGGACCAAGGTTGTG+AGG | 0.640947 | 8:-73263499 | MsG0880046427.01.T01:CDS |
| CTATGAACTCCTCATTGGTG+AGG | 0.645140 | 8:+73263882 | None:intergenic |
| TGGTTGGTTAAGAACTCATG+GGG | 0.647156 | 8:-73263141 | MsG0880046427.01.T01:CDS |
| AAGTCGACTCCATGTATGAG+AGG | 0.647740 | 8:-73264063 | MsG0880046427.01.T01:CDS |
| AGAACTCATGGGGAACTGAG+TGG | 0.653772 | 8:-73263131 | MsG0880046427.01.T01:CDS |
| TGATTGTGATACAAAAGGTG+TGG | 0.663878 | 8:-73263514 | MsG0880046427.01.T01:CDS |
| ATTTGCAGACCTCACCAATG+AGG | 0.664553 | 8:-73263891 | MsG0880046427.01.T01:CDS |
| AATGCCACAAAGTCCTTCAG+CGG | 0.670519 | 8:+73263068 | None:intergenic |
| AACACCTTGATAAGGGTATG+CGG | 0.671998 | 8:+73263416 | None:intergenic |
| GTGAAGAATCAAGGTCAATG+TGG | 0.680408 | 8:-73263734 | MsG0880046427.01.T01:intron |
| TTTCTTCCTCCAATCCACAA+CGG | 0.681262 | 8:+73263772 | None:intergenic |
| GGTGACACCATGATCCAACT+CGG | 0.706899 | 8:+73263203 | None:intergenic |
| ACCGACACCATAACCTACAG+CGG | 0.712402 | 8:+73263182 | None:intergenic |
| GATACAAAAGGTGTGGACCA+AGG | 0.723079 | 8:-73263507 | MsG0880046427.01.T01:CDS |
| GGATACATTAGGATGCGAAG+GGG | 0.731725 | 8:-73263099 | MsG0880046427.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGTAAATTACAATTAATTA+TGG | + | Chr8:73263497-73263516 | None:intergenic | 10.0% |
| !! | AAATTCATTATTCAAAATCA+TGG | - | Chr8:73263685-73263704 | MsG0880046427.01.T01:intron | 15.0% |
| !!! | AATTGTAATTTACTTTCTTT+TGG | - | Chr8:73263502-73263521 | MsG0880046427.01.T01:CDS | 15.0% |
| !!! | ACTATAGTATAACTGATTTT+TGG | + | Chr8:73262991-73263010 | None:intergenic | 20.0% |
| ! | AATAACAAGCTATACAAGTT+AGG | - | Chr8:73263215-73263234 | MsG0880046427.01.T01:CDS | 25.0% |
| ! | AGCATCTAGAAATAAATTCA+AGG | - | Chr8:73263277-73263296 | MsG0880046427.01.T01:CDS | 25.0% |
| ! | GCATCTAGAAATAAATTCAA+GGG | - | Chr8:73263278-73263297 | MsG0880046427.01.T01:CDS | 25.0% |
| !! | ATTAACATTCATTTTCTGCT+TGG | - | Chr8:73263016-73263035 | MsG0880046427.01.T01:three_prime_UTR | 25.0% |
| !! | ATTGAAGTTTTCAACAATGA+TGG | - | Chr8:73263191-73263210 | MsG0880046427.01.T01:CDS | 25.0% |
| !! | GACTTTCAATTTTACAAAAG+TGG | - | Chr8:73263889-73263908 | MsG0880046427.01.T01:CDS | 25.0% |
| !! | TTAACATTCATTTTCTGCTT+GGG | - | Chr8:73263017-73263036 | MsG0880046427.01.T01:three_prime_UTR | 25.0% |
| !!! | AATTTACTTTCTTTTGGTGT+AGG | - | Chr8:73263508-73263527 | MsG0880046427.01.T01:CDS | 25.0% |
| AAGTTTGACAATTAAGCAGT+AGG | + | Chr8:73264114-73264133 | None:intergenic | 30.0% | |
| AGTTTGACAATTAAGCAGTA+GGG | + | Chr8:73264113-73264132 | None:intergenic | 30.0% | |
| TCAATATGGCAAAGTCTATA+AGG | - | Chr8:73263115-73263134 | MsG0880046427.01.T01:CDS | 30.0% | |
| TTGCTCGAATTACAAAGTTT+AGG | + | Chr8:73264150-73264169 | None:intergenic | 30.0% | |
| ! | CTTGTTGATTGTGATACAAA+AGG | - | Chr8:73263619-73263638 | MsG0880046427.01.T01:intron | 30.0% |
| ! | GGAATTAATAAGTTGAGTAC+TGG | - | Chr8:73263568-73263587 | MsG0880046427.01.T01:CDS | 30.0% |
| AAATTGAAAGTCAGATCCAC+TGG | + | Chr8:73263881-73263900 | None:intergenic | 35.0% | |
| CTATAAGGATCCTCAAGAAA+GGG | - | Chr8:73263130-73263149 | MsG0880046427.01.T01:CDS | 35.0% | |
| GCATCAATTGCAACAGAAAT+AGG | + | Chr8:73263859-73263878 | None:intergenic | 35.0% | |
| GTGATTGACTACATTCATCA+TGG | - | Chr8:73264174-73264193 | MsG0880046427.01.T01:exon | 35.0% | |
| GTTCTTAACCAACCAATACT+TGG | + | Chr8:73263992-73264011 | None:intergenic | 35.0% | |
| TACAAAAGTGGTGTCTTTAC+CGG | - | Chr8:73263901-73263920 | MsG0880046427.01.T01:CDS | 35.0% | |
| TCACACTTCATTACCTACAA+TGG | - | Chr8:73262962-73262981 | MsG0880046427.01.T01:three_prime_UTR | 35.0% | |
| TCTATAAGGATCCTCAAGAA+AGG | - | Chr8:73263129-73263148 | MsG0880046427.01.T01:CDS | 35.0% | |
| TGATTGTGATACAAAAGGTG+TGG | - | Chr8:73263624-73263643 | MsG0880046427.01.T01:intron | 35.0% | |
| TTCCATCAACACCTTGATAA+GGG | + | Chr8:73263732-73263751 | None:intergenic | 35.0% | |
| ! | ATTGGTTGGTTAAGAACTCA+TGG | - | Chr8:73263995-73264014 | MsG0880046427.01.T01:CDS | 35.0% |
| ! | GTAATGATGGAACCAAGTAT+TGG | - | Chr8:73263977-73263996 | MsG0880046427.01.T01:CDS | 35.0% |
| ! | TTGGTTGGTTAAGAACTCAT+GGG | - | Chr8:73263996-73264015 | MsG0880046427.01.T01:CDS | 35.0% |
| !! | AGTATTTGTTTTCGCGATTG+AGG | + | Chr8:73263315-73263334 | None:intergenic | 35.0% |
| !! | TAAGTTGAGTACTGGAAAGT+TGG | - | Chr8:73263576-73263595 | MsG0880046427.01.T01:CDS | 35.0% |
| !! | TGATTTTTGGAAGCCATTGT+AGG | + | Chr8:73262978-73262997 | None:intergenic | 35.0% |
| !! | TTTTGGTGTAGGATGTTGTT+GGG | - | Chr8:73263519-73263538 | MsG0880046427.01.T01:CDS | 35.0% |
| AAGGATACATTAGGATGCGA+AGG | - | Chr8:73264037-73264056 | MsG0880046427.01.T01:CDS | 40.0% | |
| AGATGCTATGAACTCCTCAT+TGG | + | Chr8:73263264-73263283 | None:intergenic | 40.0% | |
| AGGATACATTAGGATGCGAA+GGG | - | Chr8:73264038-73264057 | MsG0880046427.01.T01:CDS | 40.0% | |
| ATTCATGCAGCTACCATTAC+CGG | - | Chr8:73263775-73263794 | MsG0880046427.01.T01:CDS | 40.0% | |
| CATTGACCTTGATTCTTCAC+AGG | + | Chr8:73263404-73263423 | None:intergenic | 40.0% | |
| GAGCAATGGATGAGTCAATA+TGG | - | Chr8:73263101-73263120 | MsG0880046427.01.T01:CDS | 40.0% | |
| GTGAAGAATCAAGGTCAATG+TGG | - | Chr8:73263404-73263423 | MsG0880046427.01.T01:CDS | 40.0% | |
| GTTACACCTGTGAAGAATCA+AGG | - | Chr8:73263395-73263414 | MsG0880046427.01.T01:CDS | 40.0% | |
| GTTCCATCAACACCTTGATA+AGG | + | Chr8:73263733-73263752 | None:intergenic | 40.0% | |
| TTTCTTCCTCCAATCCACAA+CGG | + | Chr8:73263369-73263388 | None:intergenic | 40.0% | |
| ! | AACACCTTGATAAGGGTATG+CGG | + | Chr8:73263725-73263744 | None:intergenic | 40.0% |
| ! | TGGTTGGTTAAGAACTCATG+GGG | - | Chr8:73263997-73264016 | MsG0880046427.01.T01:CDS | 40.0% |
| !! | CTTTTGGTGTAGGATGTTGT+TGG | - | Chr8:73263518-73263537 | MsG0880046427.01.T01:CDS | 40.0% |
| !! | TACCCTTATCAAGGTGTTGA+TGG | - | Chr8:73263727-73263746 | MsG0880046427.01.T01:intron | 40.0% |
| !! | TGATGGAACCAAGTATTGGT+TGG | - | Chr8:73263981-73264000 | MsG0880046427.01.T01:CDS | 40.0% |
| AATGCCACAAAGTCCTTCAG+CGG | + | Chr8:73264073-73264092 | None:intergenic | 45.0% | |
| ATTTGCAGACCTCACCAATG+AGG | - | Chr8:73263247-73263266 | MsG0880046427.01.T01:CDS | 45.0% | |
| CTATGAACTCCTCATTGGTG+AGG | + | Chr8:73263259-73263278 | None:intergenic | 45.0% | |
| GACATCTTCATACCCGGTAA+TGG | + | Chr8:73263791-73263810 | None:intergenic | 45.0% | |
| GGATACATTAGGATGCGAAG+GGG | - | Chr8:73264039-73264058 | MsG0880046427.01.T01:CDS | 45.0% | |
| GGGGTGAAGAAGGATACATT+AGG | - | Chr8:73264028-73264047 | MsG0880046427.01.T01:CDS | 45.0% | |
| TAAGGATCCTCAAGAAAGGG+AGG | - | Chr8:73263133-73263152 | MsG0880046427.01.T01:CDS | 45.0% | |
| TCTCATACATGGAGTCGACT+TGG | + | Chr8:73263076-73263095 | None:intergenic | 45.0% | |
| TCTGTTGCAATTGATGCCAG+TGG | - | Chr8:73263862-73263881 | MsG0880046427.01.T01:CDS | 45.0% | |
| TTCATGCAGCTACCATTACC+GGG | - | Chr8:73263776-73263795 | MsG0880046427.01.T01:CDS | 45.0% | |
| TTGCAATGCCTGCTCATTGT+TGG | + | Chr8:73263818-73263837 | None:intergenic | 45.0% | |
| ! | AAGTCGACTCCATGTATGAG+AGG | - | Chr8:73263075-73263094 | MsG0880046427.01.T01:CDS | 45.0% |
| ! | GATACAAAAGGTGTGGACCA+AGG | - | Chr8:73263631-73263650 | MsG0880046427.01.T01:intron | 45.0% |
| ! | GGTAATGGTAGCTGCATGAA+TGG | + | Chr8:73263776-73263795 | None:intergenic | 45.0% |
| !! | GGTTATGGTGTCGGTAATGA+TGG | - | Chr8:73263964-73263983 | MsG0880046427.01.T01:CDS | 45.0% |
| !! | GTTGTGGATTGGAGGAAGAA+AGG | - | Chr8:73263368-73263387 | MsG0880046427.01.T01:CDS | 45.0% |
| AACTCATGGGGAACTGAGTG+GGG | - | Chr8:73264009-73264028 | MsG0880046427.01.T01:CDS | 50.0% | |
| AATGCCTGCTCATTGTTGGC+AGG | + | Chr8:73263814-73263833 | None:intergenic | 50.0% | |
| ACCGACACCATAACCTACAG+CGG | + | Chr8:73263959-73263978 | None:intergenic | 50.0% | |
| ACCGCTGTAGGTTATGGTGT+CGG | - | Chr8:73263955-73263974 | MsG0880046427.01.T01:CDS | 50.0% | |
| AGAACTCATGGGGAACTGAG+TGG | - | Chr8:73264007-73264026 | MsG0880046427.01.T01:CDS | 50.0% | |
| AGACGTTCCTCCCTTTCTTG+AGG | + | Chr8:73263143-73263162 | None:intergenic | 50.0% | |
| ATGCCTGCTCATTGTTGGCA+GGG | + | Chr8:73263813-73263832 | None:intergenic | 50.0% | |
| CAACTCGGTTCCACATGAAC+CGG | + | Chr8:73263923-73263942 | None:intergenic | 50.0% | |
| GAACTCATGGGGAACTGAGT+GGG | - | Chr8:73264008-73264027 | MsG0880046427.01.T01:CDS | 50.0% | |
| GAAGCCGCATACCCTTATCA+AGG | - | Chr8:73263718-73263737 | MsG0880046427.01.T01:intron | 50.0% | |
| GGTGACACCATGATCCAACT+CGG | + | Chr8:73263938-73263957 | None:intergenic | 50.0% | |
| TAAGACCACCCTCACAACCT+TGG | + | Chr8:73263651-73263670 | None:intergenic | 50.0% | |
| TTCCTCCAATCCACAACGGA+CGG | + | Chr8:73263365-73263384 | None:intergenic | 50.0% | |
| TTGCTCGTGCCTCTCATACA+TGG | + | Chr8:73263087-73263106 | None:intergenic | 50.0% | |
| ! | AGGTTGTGAGGGTGGTCTTA+TGG | - | Chr8:73263651-73263670 | MsG0880046427.01.T01:intron | 50.0% |
| ! | TGTATGAGAGGCACGAGCAA+TGG | - | Chr8:73263087-73263106 | MsG0880046427.01.T01:CDS | 50.0% |
| ! | TGTGGAACCGAGTTGGATCA+TGG | - | Chr8:73263928-73263947 | MsG0880046427.01.T01:CDS | 50.0% |
| !! | GGTGTCTTTACCGGTTCATG+TGG | - | Chr8:73263910-73263929 | MsG0880046427.01.T01:CDS | 50.0% |
| AAGTGCAATCCCGTCCGTTG+TGG | - | Chr8:73263352-73263371 | MsG0880046427.01.T01:CDS | 55.0% | |
| AGGTGTGGACCAAGGTTGTG+AGG | - | Chr8:73263639-73263658 | MsG0880046427.01.T01:intron | 55.0% | |
| CAATCCCGTCCGTTGTGGAT+TGG | - | Chr8:73263357-73263376 | MsG0880046427.01.T01:CDS | 55.0% | |
| CGGTTCATGTGGAACCGAGT+TGG | - | Chr8:73263921-73263940 | MsG0880046427.01.T01:CDS | 55.0% | |
| GATGCCGCTGAAGGACTTTG+TGG | - | Chr8:73264066-73264085 | MsG0880046427.01.T01:CDS | 55.0% | |
| GGAACTGAGTGGGGTGAAGA+AGG | - | Chr8:73264018-73264037 | MsG0880046427.01.T01:CDS | 55.0% | |
| GGCAGGGACATCTTCATACC+CGG | + | Chr8:73263797-73263816 | None:intergenic | 55.0% | |
| GGTGTGGACCAAGGTTGTGA+GGG | - | Chr8:73263640-73263659 | MsG0880046427.01.T01:intron | 55.0% | |
| TCAGCTGTTGCAGCGACTGA+AGG | - | Chr8:73263547-73263566 | MsG0880046427.01.T01:CDS | 55.0% | |
| TCCTCCAATCCACAACGGAC+GGG | + | Chr8:73263364-73263383 | None:intergenic | 55.0% | |
| TGTCCCTGCCAACAATGAGC+AGG | - | Chr8:73263807-73263826 | MsG0880046427.01.T01:CDS | 55.0% | |
| ! | GGTGTCACCGCTGTAGGTTA+TGG | - | Chr8:73263949-73263968 | MsG0880046427.01.T01:CDS | 55.0% |
| !! | GATCATGGTGTCACCGCTGT+AGG | - | Chr8:73263943-73263962 | MsG0880046427.01.T01:CDS | 55.0% |
| GTGGACCAAGGTTGTGAGGG+TGG | - | Chr8:73263643-73263662 | MsG0880046427.01.T01:intron | 60.0% | |
| !! | AGGGGAGTTGATGCCGCTGA+AGG | - | Chr8:73264057-73264076 | MsG0880046427.01.T01:CDS | 60.0% |
| !! | TCCCGTCCGTTGTGGATTGG+AGG | - | Chr8:73263360-73263379 | MsG0880046427.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 73262905 | 73264255 | 73262905 | ID=MsG0880046427.01;Name=MsG0880046427.01 |
| Chr8 | mRNA | 73262905 | 73264255 | 73262905 | ID=MsG0880046427.01.T01;Parent=MsG0880046427.01;Name=MsG0880046427.01.T01;_AED=0.44;_eAED=0.44;_QI=76|1|1|1|1|1|2|133|345 |
| Chr8 | exon | 73262905 | 73263630 | 73262905 | ID=MsG0880046427.01.T01:exon:18969;Parent=MsG0880046427.01.T01 |
| Chr8 | exon | 73263735 | 73264255 | 73263735 | ID=MsG0880046427.01.T01:exon:18968;Parent=MsG0880046427.01.T01 |
| Chr8 | five_prime_UTR | 73264180 | 73264255 | 73264180 | ID=MsG0880046427.01.T01:five_prime_utr;Parent=MsG0880046427.01.T01 |
| Chr8 | CDS | 73263735 | 73264179 | 73263735 | ID=MsG0880046427.01.T01:cds;Parent=MsG0880046427.01.T01 |
| Chr8 | CDS | 73263038 | 73263630 | 73263038 | ID=MsG0880046427.01.T01:cds;Parent=MsG0880046427.01.T01 |
| Chr8 | three_prime_UTR | 73262905 | 73263037 | 73262905 | ID=MsG0880046427.01.T01:three_prime_utr;Parent=MsG0880046427.01.T01 |
| Gene Sequence |
| Protein sequence |
