AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004590.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004590.01.T01 MTR_1g083190 96.646 477 11 1 1 477 1 472 0.0 926
MsG0180004590.01.T01 MTR_3g106420 93.958 480 26 1 1 477 1 480 0.0 887
MsG0180004590.01.T01 MTR_3g106480 90.377 478 43 1 3 477 1 478 0.0 875
MsG0180004590.01.T01 MTR_3g106485 91.158 475 41 1 3 477 1 474 0.0 848
MsG0180004590.01.T01 MTR_3g106430 92.194 474 36 1 3 476 1 473 0.0 830
MsG0180004590.01.T01 MTR_1g073400 89.263 475 50 1 3 477 1 474 0.0 818
MsG0180004590.01.T01 MTR_1g071130 83.789 475 51 1 3 477 1 449 0.0 810
MsG0180004590.01.T01 MTR_1g115145 86.667 480 52 5 3 477 1 473 0.0 808
MsG0180004590.01.T01 MTR_3g106470 82.809 477 23 3 3 476 32 452 0.0 744
MsG0180004590.01.T01 MTR_8g063830 79.741 464 52 5 3 463 1 425 0.0 657
MsG0180004590.01.T01 MTR_3g065540 62.609 115 16 1 42 156 14 101 6.39e-38 134
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004590.01.T01 AT5G25260 72.881 472 115 2 3 472 1 461 0.0 710
MsG0180004590.01.T01 AT5G25250 72.766 470 117 1 3 472 1 459 0.0 705
MsG0180004590.01.T01 AT5G64870 70.551 472 125 2 4 472 3 463 0.0 647

Find 122 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGAGTATTTCTCATATT+TGG 0.119823 1:-80061859 MsG0180004590.01.T01:CDS
TTGAGCCTAAGATTAGTATT+TGG 0.251063 1:-80060683 MsG0180004590.01.T01:CDS
CATGAGTATTTCTCATATTT+GGG 0.292202 1:-80061858 MsG0180004590.01.T01:CDS
AAACTCTTAATTAGGTTTAG+AGG 0.304122 1:-80060514 MsG0180004590.01.T01:exon
AACAAGTTCATTAACATGAT+TGG 0.311801 1:+80062058 None:intergenic
CGAAGAAGGCTGTGGCTCTA+AGG 0.317541 1:-80061530 MsG0180004590.01.T01:CDS
GCTAAGATGAAAGGTGAAAT+TGG 0.319472 1:-80061783 MsG0180004590.01.T01:CDS
GTCAACCCTAGCTTGATTCT+TGG 0.329722 1:+80061815 None:intergenic
AAGACAGCGGGGAGAATAAA+AGG 0.329818 1:+80062158 None:intergenic
AGAGCTTTATGCAAAGAAAA+AGG 0.337974 1:-80060868 MsG0180004590.01.T01:CDS
AGGGATTACTTGATGATAAA+TGG 0.347159 1:-80060756 MsG0180004590.01.T01:CDS
CAACTTGAGTTGAATCAATT+CGG 0.350972 1:-80061930 MsG0180004590.01.T01:CDS
ACTAACGGTGGCGGCGATAA+TGG 0.358246 1:-80060660 MsG0180004590.01.T01:CDS
TCAAAACCATGAAAATTTAC+CGG 0.364824 1:-80062343 MsG0180004590.01.T01:exon
TGTAGGTACAAGAAGCAAAC+TGG 0.373363 1:-80061010 MsG0180004590.01.T01:intron
ACTTCGAAGGTGTAGTTTAC+CGG 0.380695 1:+80062206 None:intergenic
GTGAAATTGGTTCAAAATTG+AGG 0.384625 1:-80061770 MsG0180004590.01.T01:CDS
TTAGTCCAAATACTAATCTT+AGG 0.384650 1:+80060678 None:intergenic
CGGTCAATCCTGTTCTGTCT+TGG 0.385218 1:-80062237 MsG0180004590.01.T01:CDS
CAATGCGAATGTTAAGCAAT+TGG 0.388576 1:-80061895 MsG0180004590.01.T01:CDS
ATAGTAGTAGTACTTGGTTT+TGG 0.403199 1:-80060488 MsG0180004590.01.T01:three_prime_UTR
TCAGAATACCTTGTGATAAC+AGG 0.405430 1:-80062308 MsG0180004590.01.T01:CDS
GGCTTGGACCAAGGCGGCTC+AGG 0.413477 1:-80061568 MsG0180004590.01.T01:CDS
CATGTTAATGAACTTGTTCA+AGG 0.419850 1:-80062053 MsG0180004590.01.T01:CDS
TTCAGCCACCTGAGCCGCCT+TGG 0.421816 1:+80061560 None:intergenic
GGCTGGGGAGGGTGACAAGG+AGG 0.433381 1:-80061682 MsG0180004590.01.T01:CDS
TGAAATTGGTTCAAAATTGA+GGG 0.433449 1:-80061769 MsG0180004590.01.T01:CDS
AAAGAGGTAATAAATTGAAT+TGG 0.436538 1:-80060224 MsG0180004590.01.T01:three_prime_UTR
TTATTGCGATGCAGAGGGCT+GGG 0.452498 1:-80061698 MsG0180004590.01.T01:CDS
CGGTCCTCGTGTAGATGATA+AGG 0.455566 1:-80062129 MsG0180004590.01.T01:CDS
AGCATGCGGTGAAATAAGTT+TGG 0.456709 1:+80062088 None:intergenic
GTTATTGCGATGCAGAGGGC+TGG 0.467250 1:-80061699 MsG0180004590.01.T01:CDS
GTAACATCTCCGTTTGCTCA+TGG 0.473481 1:+80060568 None:intergenic
TGCGATGCAGAGGGCTGGGG+AGG 0.475380 1:-80061694 MsG0180004590.01.T01:CDS
AAGAAAGCATGGATTATTCC+CGG 0.476347 1:-80062257 MsG0180004590.01.T01:CDS
TTGGACCAAGGCGGCTCAGG+TGG 0.476684 1:-80061565 MsG0180004590.01.T01:CDS
GATGTTACCACCTGCTTGGA+TGG 0.478172 1:-80060553 MsG0180004590.01.T01:CDS
CTCCCCGCTGTCTTCACTAT+CGG 0.478414 1:-80062149 MsG0180004590.01.T01:CDS
GAGAATAAAAGGAAGTTTCT+CGG 0.481453 1:+80062169 None:intergenic
GAAACAACTACACAGCTGTA+AGG 0.490449 1:-80060776 MsG0180004590.01.T01:CDS
AGGTTTATAGTAGTAGTACT+TGG 0.490760 1:-80060494 MsG0180004590.01.T01:three_prime_UTR
TGAGAGGTCCAAGACAGAAC+AGG 0.492527 1:+80062229 None:intergenic
CAAGACAGAACAGGATTGAC+CGG 0.494108 1:+80062238 None:intergenic
CAAAACCATGAAAATTTACC+GGG 0.508642 1:-80062342 MsG0180004590.01.T01:exon
AGCAATTCTGTATGAGAAGA+AGG 0.509643 1:-80060958 MsG0180004590.01.T01:CDS
AGTACACTTCTTAATGCACT+TGG 0.511750 1:-80060798 MsG0180004590.01.T01:CDS
TGCTTCCATGACGATGGAGG+AGG 0.512100 1:-80062000 MsG0180004590.01.T01:CDS
GGACCGATAGTGAAGACAGC+GGG 0.513792 1:+80062146 None:intergenic
CGATAATGGTGAAGGTGCCA+TGG 0.514200 1:-80060646 MsG0180004590.01.T01:CDS
TTCTGTCTTGGACCTCTCAC+CGG 0.515981 1:-80062225 MsG0180004590.01.T01:CDS
GAGGGCTGGGGAGGGTGACA+AGG 0.520045 1:-80061685 MsG0180004590.01.T01:CDS
GGCTGAAGTCGAGGCGAAGA+AGG 0.523986 1:-80061544 MsG0180004590.01.T01:CDS
GCGATGCAGAGGGCTGGGGA+GGG 0.526835 1:-80061693 MsG0180004590.01.T01:CDS
CGGAGATGTTACCACCTGCT+TGG 0.527198 1:-80060557 MsG0180004590.01.T01:CDS
TAAGTTTGGCGTATTTGAGA+AGG 0.534048 1:+80062102 None:intergenic
AGCAACTTCTTTCATACCCA+TGG 0.534773 1:+80060629 None:intergenic
GGCAAGACTCCCATCCAAGC+AGG 0.534806 1:+80060543 None:intergenic
CTAAGGGAAGCTGAGTTACA+AGG 0.541402 1:-80061513 MsG0180004590.01.T01:CDS
GGCTCAAGCTAATTCTGAGT+TGG 0.541784 1:-80061604 MsG0180004590.01.T01:CDS
GAAGAAGGCTGTGGCTCTAA+GGG 0.545005 1:-80061529 MsG0180004590.01.T01:CDS
TATTGCGATGCAGAGGGCTG+GGG 0.546542 1:-80061697 MsG0180004590.01.T01:CDS
TGCTGCTTCCATGACGATGG+AGG 0.547408 1:-80062003 MsG0180004590.01.T01:CDS
TAAGACCGTCCATGAGCAAA+CGG 0.547678 1:-80060577 MsG0180004590.01.T01:CDS
TGGAAGCCAAGAATCAAGCT+AGG 0.548842 1:-80061821 MsG0180004590.01.T01:CDS
GTGACACTTGGACAAGCTCA+AGG 0.553312 1:-80060831 MsG0180004590.01.T01:CDS
AGTCGAGGCGAAGAAGGCTG+TGG 0.558891 1:-80061538 MsG0180004590.01.T01:CDS
GGCTCAGGTGGCTGAAGTCG+AGG 0.562422 1:-80061553 MsG0180004590.01.T01:CDS
TGCGAATGTTAAGCAATTGG+TGG 0.563120 1:-80061892 MsG0180004590.01.T01:CDS
AAGATTAGTATTTGGACTAA+CGG 0.563509 1:-80060675 MsG0180004590.01.T01:CDS
ATTGAACACTAGCTTTGCTA+AGG 0.566032 1:+80061436 None:intergenic
GAAGAAAGCGGCTTGGACCA+AGG 0.572319 1:-80061577 MsG0180004590.01.T01:CDS
TAAAACTCGCAAAGAAAGCA+TGG 0.572673 1:-80062268 MsG0180004590.01.T01:CDS
GAAAACCTCCTCCATCGTCA+TGG 0.573319 1:+80061995 None:intergenic
ATGTTACCACCTGCTTGGAT+GGG 0.573544 1:-80060552 MsG0180004590.01.T01:CDS
GCAGAGGGAATTGTGACACT+TGG 0.575919 1:-80060843 MsG0180004590.01.T01:CDS
ATGGGTATGAAAGAAGTTGC+TGG 0.579940 1:-80060627 MsG0180004590.01.T01:CDS
ATTGGTTCAAAATTGAGGGA+AGG 0.580771 1:-80061765 MsG0180004590.01.T01:CDS
TCTTGCTGCTTCCATGACGA+TGG 0.585164 1:-80062006 MsG0180004590.01.T01:CDS
TGGCTAAGAAGAAAGCGGCT+TGG 0.585206 1:-80061584 MsG0180004590.01.T01:CDS
AAACAACTACACAGCTGTAA+GGG 0.585417 1:-80060775 MsG0180004590.01.T01:CDS
CGAAGGTTATTGCGATGCAG+AGG 0.587186 1:-80061704 MsG0180004590.01.T01:CDS
GCTGGGGAGGGTGACAAGGA+GGG 0.589298 1:-80061681 MsG0180004590.01.T01:CDS
GAATCAAAGAGAGGCTGAAG+TGG 0.589954 1:-80061625 MsG0180004590.01.T01:CDS
GGCAGAAGCACAGAAAGCAT+CGG 0.592056 1:-80060931 MsG0180004590.01.T01:CDS
TGTTGACGTTGTGTCTAAAG+AGG 0.594645 1:-80060240 MsG0180004590.01.T01:three_prime_UTR
GATAATGGTGAAGGTGCCAT+GGG 0.600255 1:-80060645 MsG0180004590.01.T01:CDS
TCTGTATGAGAAGAAGGCAG+AGG 0.600808 1:-80060952 MsG0180004590.01.T01:CDS
TCATGGACGGTCTTAAACAA+AGG 0.601527 1:+80060585 None:intergenic
ACTCATAGCTTGAACTTCGA+AGG 0.602674 1:+80062193 None:intergenic
AGTTACAAGGTGAAGTTGAG+AGG 0.609202 1:-80061500 MsG0180004590.01.T01:CDS
TGACACTTGGACAAGCTCAA+GGG 0.610411 1:-80060830 MsG0180004590.01.T01:CDS
TTTGGGTCAAAAGACGCAGA+TGG 0.613836 1:-80061841 MsG0180004590.01.T01:CDS
GTGGCAGAAGCTAAGATGAA+AGG 0.614706 1:-80061792 MsG0180004590.01.T01:CDS
GACACTTGGACAAGCTCAAG+GGG 0.615609 1:-80060829 MsG0180004590.01.T01:CDS
AGGTGTAGTTTACCGGTGAG+AGG 0.619207 1:+80062213 None:intergenic
GATTGCTAAGATTAATGCTG+AGG 0.620636 1:-80060718 MsG0180004590.01.T01:CDS
AGTATTTGGACTAACGGTGG+CGG 0.622001 1:-80060669 MsG0180004590.01.T01:CDS
GTAGGTACAAGAAGCAAACT+GGG 0.627939 1:-80061009 MsG0180004590.01.T01:intron
CATCTCCGTTTGCTCATGGA+CGG 0.630434 1:+80060572 None:intergenic
TGAGTTGGCTAAGAAGAAAG+CGG 0.631786 1:-80061589 MsG0180004590.01.T01:CDS
GAAGGTTATTGCGATGCAGA+GGG 0.633633 1:-80061703 MsG0180004590.01.T01:CDS
CTTGTTCAAGGAATCATCGA+AGG 0.639303 1:-80062041 MsG0180004590.01.T01:CDS
GGAAGCCAAGAATCAAGCTA+GGG 0.640292 1:-80061820 MsG0180004590.01.T01:CDS
TAGTGTTCAATATGAAACCA+AGG 0.651992 1:-80061424 MsG0180004590.01.T01:intron
CAAAGACTAACATCACATTG+TGG 0.652496 1:-80060339 MsG0180004590.01.T01:three_prime_UTR
GAAAATTGATGCAGAGACGA+AGG 0.652587 1:-80061721 MsG0180004590.01.T01:CDS
GAATCAAGCTAGGGTTGACG+TGG 0.658059 1:-80061811 MsG0180004590.01.T01:CDS
GGTGGCGGCGATAATGGTGA+AGG 0.658257 1:-80060654 MsG0180004590.01.T01:CDS
GATTACTTGATGATAAATGG+TGG 0.658453 1:-80060753 MsG0180004590.01.T01:CDS
TGGACGGTCTTAAACAAAGG+TGG 0.660087 1:+80060588 None:intergenic
AAGACAGAACAGGATTGACC+GGG 0.664744 1:+80062239 None:intergenic
ATTAGTATTTGGACTAACGG+TGG 0.669931 1:-80060672 MsG0180004590.01.T01:CDS
TCAAAATACCTGTTATCACA+AGG 0.672413 1:+80062300 None:intergenic
AAGACTCCCATCCAAGCAGG+TGG 0.679480 1:+80060546 None:intergenic
CTTTCCTTATCATCTACACG+AGG 0.686856 1:+80062125 None:intergenic
ATTAATGCTGAGGCAATACG+CGG 0.688568 1:-80060708 MsG0180004590.01.T01:CDS
AAGCAATTGGTGGATGTGCG+CGG 0.690257 1:-80061882 MsG0180004590.01.T01:CDS
ACAAGGAGGGAATTAAAGTG+AGG 0.693697 1:-80061668 MsG0180004590.01.T01:CDS
GACCGATAGTGAAGACAGCG+GGG 0.713902 1:+80062147 None:intergenic
GAAAGCGGCTTGGACCAAGG+CGG 0.735762 1:-80061574 MsG0180004590.01.T01:CDS
AGGACCGATAGTGAAGACAG+CGG 0.753084 1:+80062145 None:intergenic
TGATTGGAGTGTCTAGCATG+CGG 0.779262 1:+80062074 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAAATTGAATTTGCA+TGG + Chr1:80061252-80061271 None:intergenic 15.0%
!!! AATTTTAAAAGACTAATGAA+AGG - Chr1:80061343-80061362 MsG0180004590.01.T01:intron 15.0%
!!! ACATTACACTATTATTTTTT+TGG - Chr1:80061584-80061603 MsG0180004590.01.T01:CDS 15.0%
!!! AGTACTTTTTTATTAGTTTT+GGG - Chr1:80062187-80062206 MsG0180004590.01.T01:CDS 15.0%
!!! TAGTACTTTTTTATTAGTTT+TGG - Chr1:80062186-80062205 MsG0180004590.01.T01:CDS 15.0%
!!! TCATTATTGATTATGTTATA+TGG - Chr1:80061426-80061445 MsG0180004590.01.T01:CDS 15.0%
!!! TGTTTTTTTAAATGGAATTT+TGG - Chr1:80062258-80062277 MsG0180004590.01.T01:CDS 15.0%
!! AAAGAGGTAATAAATTGAAT+TGG - Chr1:80062416-80062435 MsG0180004590.01.T01:five_prime_UTR 20.0%
!!! TAGATGCTTGTTTTTTTAAA+TGG - Chr1:80062250-80062269 MsG0180004590.01.T01:CDS 20.0%
!!! TTTTTTGGTCATTAAAACTT+TGG - Chr1:80061599-80061618 MsG0180004590.01.T01:CDS 20.0%
! AAACTCTTAATTAGGTTTAG+AGG - Chr1:80062126-80062145 MsG0180004590.01.T01:CDS 25.0%
! AACAAGTTCATTAACATGAT+TGG + Chr1:80060585-80060604 None:intergenic 25.0%
! AAGATTAGTATTTGGACTAA+CGG - Chr1:80061965-80061984 MsG0180004590.01.T01:CDS 25.0%
! CATGAGTATTTCTCATATTT+GGG - Chr1:80060782-80060801 MsG0180004590.01.T01:CDS 25.0%
! TAATGTAACAAAGATTAAGC+AGG + Chr1:80061570-80061589 None:intergenic 25.0%
! TCAAAACCATGAAAATTTAC+CGG - Chr1:80060297-80060316 MsG0180004590.01.T01:three_prime_UTR 25.0%
! TCATGAGTATTTCTCATATT+TGG - Chr1:80060781-80060800 MsG0180004590.01.T01:CDS 25.0%
! TTAGTCCAAATACTAATCTT+AGG + Chr1:80061965-80061984 None:intergenic 25.0%
!! TGAAATTGGTTCAAAATTGA+GGG - Chr1:80060871-80060890 MsG0180004590.01.T01:CDS 25.0%
!!! CATGAGTATTGAAAATTCAA+TGG - Chr1:80061543-80061562 MsG0180004590.01.T01:CDS 25.0%
AGGTTTATAGTAGTAGTACT+TGG - Chr1:80062146-80062165 MsG0180004590.01.T01:CDS 30.0%
CAAAACCATGAAAATTTACC+GGG - Chr1:80060298-80060317 MsG0180004590.01.T01:three_prime_UTR 30.0%
CCTGACAAAAACTCTTAATT+AGG - Chr1:80062118-80062137 MsG0180004590.01.T01:CDS 30.0%
GAGAATAAAAGGAAGTTTCT+CGG + Chr1:80060474-80060493 None:intergenic 30.0%
TAGTGTTCAATATGAAACCA+AGG - Chr1:80061216-80061235 MsG0180004590.01.T01:intron 30.0%
TCAAAATACCTGTTATCACA+AGG + Chr1:80060343-80060362 None:intergenic 30.0%
TTGAGCCTAAGATTAGTATT+TGG - Chr1:80061957-80061976 MsG0180004590.01.T01:CDS 30.0%
TTGGTCATTAAAACTTTGGA+TGG - Chr1:80061603-80061622 MsG0180004590.01.T01:CDS 30.0%
! AGAGCTTTATGCAAAGAAAA+AGG - Chr1:80061772-80061791 MsG0180004590.01.T01:CDS 30.0%
! AGGGATTACTTGATGATAAA+TGG - Chr1:80061884-80061903 MsG0180004590.01.T01:CDS 30.0%
! ATAGTAGTAGTACTTGGTTT+TGG - Chr1:80062152-80062171 MsG0180004590.01.T01:CDS 30.0%
! CATGTTAATGAACTTGTTCA+AGG - Chr1:80060587-80060606 MsG0180004590.01.T01:CDS 30.0%
! CCTAATTAAGAGTTTTTGTC+AGG + Chr1:80062121-80062140 None:intergenic 30.0%
! GATTACTTGATGATAAATGG+TGG - Chr1:80061887-80061906 MsG0180004590.01.T01:CDS 30.0%
! GTGAAATTGGTTCAAAATTG+AGG - Chr1:80060870-80060889 MsG0180004590.01.T01:CDS 30.0%
!! AGTTTTTGAGAATCAAAGAG+AGG - Chr1:80061006-80061025 MsG0180004590.01.T01:CDS 30.0%
!! CAACTTGAGTTGAATCAATT+CGG - Chr1:80060710-80060729 MsG0180004590.01.T01:CDS 30.0%
AAACAACTACACAGCTGTAA+GGG - Chr1:80061865-80061884 MsG0180004590.01.T01:CDS 35.0%
AAACTTTGGATGGTGTATGT+AGG - Chr1:80061613-80061632 MsG0180004590.01.T01:CDS 35.0%
AGCAATTCTGTATGAGAAGA+AGG - Chr1:80061682-80061701 MsG0180004590.01.T01:CDS 35.0%
AGTACACTTCTTAATGCACT+TGG - Chr1:80061842-80061861 MsG0180004590.01.T01:CDS 35.0%
ATTAGTATTTGGACTAACGG+TGG - Chr1:80061968-80061987 MsG0180004590.01.T01:CDS 35.0%
CAAAGACTAACATCACATTG+TGG - Chr1:80062301-80062320 MsG0180004590.01.T01:CDS 35.0%
CAATGCGAATGTTAAGCAAT+TGG - Chr1:80060745-80060764 MsG0180004590.01.T01:CDS 35.0%
GATTGCTAAGATTAATGCTG+AGG - Chr1:80061922-80061941 MsG0180004590.01.T01:CDS 35.0%
GCTAAGATGAAAGGTGAAAT+TGG - Chr1:80060857-80060876 MsG0180004590.01.T01:CDS 35.0%
GCTGTACAAAAAACAGAAAG+AGG - Chr1:80061655-80061674 MsG0180004590.01.T01:CDS 35.0%
GCTTTATGCAAAGAAAAAGG+AGG - Chr1:80061775-80061794 MsG0180004590.01.T01:CDS 35.0%
TAAAACTCGCAAAGAAAGCA+TGG - Chr1:80060372-80060391 MsG0180004590.01.T01:three_prime_UTR 35.0%
TAAGTTTGGCGTATTTGAGA+AGG + Chr1:80060541-80060560 None:intergenic 35.0%
TCAGAATACCTTGTGATAAC+AGG - Chr1:80060332-80060351 MsG0180004590.01.T01:three_prime_UTR 35.0%
! AAGAAAGCATGGATTATTCC+CGG - Chr1:80060383-80060402 MsG0180004590.01.T01:three_prime_UTR 35.0%
! ATTGGTTCAAAATTGAGGGA+AGG - Chr1:80060875-80060894 MsG0180004590.01.T01:CDS 35.0%
! GAAAGGATAGACAACTTTTG+AGG - Chr1:80061360-80061379 MsG0180004590.01.T01:intron 35.0%
!! ATTGAACACTAGCTTTGCTA+AGG + Chr1:80061207-80061226 None:intergenic 35.0%
!! GTTTGAACTGTTTTGTTCCT+TGG + Chr1:80060670-80060689 None:intergenic 35.0%
ACAAGGAGGGAATTAAAGTG+AGG - Chr1:80060972-80060991 MsG0180004590.01.T01:CDS 40.0%
ACTCATAGCTTGAACTTCGA+AGG + Chr1:80060450-80060469 None:intergenic 40.0%
AGCAACTTCTTTCATACCCA+TGG + Chr1:80062014-80062033 None:intergenic 40.0%
AGCATGCGGTGAAATAAGTT+TGG + Chr1:80060555-80060574 None:intergenic 40.0%
AGTTACAAGGTGAAGTTGAG+AGG - Chr1:80061140-80061159 MsG0180004590.01.T01:intron 40.0%
ATGGGTATGAAAGAAGTTGC+TGG - Chr1:80062013-80062032 MsG0180004590.01.T01:CDS 40.0%
ATTAATGCTGAGGCAATACG+CGG - Chr1:80061932-80061951 MsG0180004590.01.T01:CDS 40.0%
CTTGTTCAAGGAATCATCGA+AGG - Chr1:80060599-80060618 MsG0180004590.01.T01:CDS 40.0%
CTTTCCTTATCATCTACACG+AGG + Chr1:80060518-80060537 None:intergenic 40.0%
GAAAATTGATGCAGAGACGA+AGG - Chr1:80060919-80060938 MsG0180004590.01.T01:CDS 40.0%
GAAACAACTACACAGCTGTA+AGG - Chr1:80061864-80061883 MsG0180004590.01.T01:CDS 40.0%
GTAGGTACAAGAAGCAAACT+GGG - Chr1:80061631-80061650 MsG0180004590.01.T01:CDS 40.0%
TCATGGACGGTCTTAAACAA+AGG + Chr1:80062058-80062077 None:intergenic 40.0%
TGCGAATGTTAAGCAATTGG+TGG - Chr1:80060748-80060767 MsG0180004590.01.T01:CDS 40.0%
TGTAGGTACAAGAAGCAAAC+TGG - Chr1:80061630-80061649 MsG0180004590.01.T01:CDS 40.0%
TGTTGACGTTGTGTCTAAAG+AGG - Chr1:80062400-80062419 MsG0180004590.01.T01:five_prime_UTR 40.0%
! ACTTCGAAGGTGTAGTTTAC+CGG + Chr1:80060437-80060456 None:intergenic 40.0%
! ATTCTGATGCTTTTGCAACC+CGG + Chr1:80060319-80060338 None:intergenic 40.0%
! TGAGTTGGCTAAGAAGAAAG+CGG - Chr1:80061051-80061070 MsG0180004590.01.T01:intron 40.0%
! TGCAACCCGGTAAATTTTCA+TGG + Chr1:80060306-80060325 None:intergenic 40.0%
! TGCATGGACTAAACTAACCT+TGG + Chr1:80061236-80061255 None:intergenic 40.0%
AAGACAGAACAGGATTGACC+GGG + Chr1:80060404-80060423 None:intergenic 45.0%
AAGACAGCGGGGAGAATAAA+AGG + Chr1:80060485-80060504 None:intergenic 45.0%
AGTATTTGGACTAACGGTGG+CGG - Chr1:80061971-80061990 MsG0180004590.01.T01:CDS 45.0%
ATGTTACCACCTGCTTGGAT+GGG - Chr1:80062088-80062107 MsG0180004590.01.T01:CDS 45.0%
CAAGACAGAACAGGATTGAC+CGG + Chr1:80060405-80060424 None:intergenic 45.0%
CTAAGGGAAGCTGAGTTACA+AGG - Chr1:80061127-80061146 MsG0180004590.01.T01:intron 45.0%
GAAGGTTATTGCGATGCAGA+GGG - Chr1:80060937-80060956 MsG0180004590.01.T01:CDS 45.0%
GAATCAAAGAGAGGCTGAAG+TGG - Chr1:80061015-80061034 MsG0180004590.01.T01:intron 45.0%
GCAAAGAAAAAGGAGGCAGA+GGG - Chr1:80061782-80061801 MsG0180004590.01.T01:CDS 45.0%
GGAAGCCAAGAATCAAGCTA+GGG - Chr1:80060820-80060839 MsG0180004590.01.T01:CDS 45.0%
GGCTCAAGCTAATTCTGAGT+TGG - Chr1:80061036-80061055 MsG0180004590.01.T01:intron 45.0%
GTAACATCTCCGTTTGCTCA+TGG + Chr1:80062075-80062094 None:intergenic 45.0%
GTCAACCCTAGCTTGATTCT+TGG + Chr1:80060828-80060847 None:intergenic 45.0%
GTGGCAGAAGCTAAGATGAA+AGG - Chr1:80060848-80060867 MsG0180004590.01.T01:CDS 45.0%
TAAGACCGTCCATGAGCAAA+CGG - Chr1:80062063-80062082 MsG0180004590.01.T01:CDS 45.0%
TCTGTATGAGAAGAAGGCAG+AGG - Chr1:80061688-80061707 MsG0180004590.01.T01:CDS 45.0%
TGACACTTGGACAAGCTCAA+GGG - Chr1:80061810-80061829 MsG0180004590.01.T01:CDS 45.0%
TGCAAAGAAAAAGGAGGCAG+AGG - Chr1:80061781-80061800 MsG0180004590.01.T01:CDS 45.0%
TGGAAGCCAAGAATCAAGCT+AGG - Chr1:80060819-80060838 MsG0180004590.01.T01:CDS 45.0%
TGGACGGTCTTAAACAAAGG+TGG + Chr1:80062055-80062074 None:intergenic 45.0%
TTTGGGTCAAAAGACGCAGA+TGG - Chr1:80060799-80060818 MsG0180004590.01.T01:CDS 45.0%
! TGATTGGAGTGTCTAGCATG+CGG + Chr1:80060569-80060588 None:intergenic 45.0%
!! GATAATGGTGAAGGTGCCAT+GGG - Chr1:80061995-80062014 MsG0180004590.01.T01:CDS 45.0%
AGGACCGATAGTGAAGACAG+CGG + Chr1:80060498-80060517 None:intergenic 50.0%
AGGTGTAGTTTACCGGTGAG+AGG + Chr1:80060430-80060449 None:intergenic 50.0%
CATCTCCGTTTGCTCATGGA+CGG + Chr1:80062071-80062090 None:intergenic 50.0%
CGAAGGTTATTGCGATGCAG+AGG - Chr1:80060936-80060955 MsG0180004590.01.T01:CDS 50.0%
CGGTCAATCCTGTTCTGTCT+TGG - Chr1:80060403-80060422 MsG0180004590.01.T01:three_prime_UTR 50.0%
CGGTCCTCGTGTAGATGATA+AGG - Chr1:80060511-80060530 MsG0180004590.01.T01:exon 50.0%
GAAAACCTCCTCCATCGTCA+TGG + Chr1:80060648-80060667 None:intergenic 50.0%
GAATCAAGCTAGGGTTGACG+TGG - Chr1:80060829-80060848 MsG0180004590.01.T01:CDS 50.0%
GACACTTGGACAAGCTCAAG+GGG - Chr1:80061811-80061830 MsG0180004590.01.T01:CDS 50.0%
GATGTTACCACCTGCTTGGA+TGG - Chr1:80062087-80062106 MsG0180004590.01.T01:CDS 50.0%
GCAGAGGGAATTGTGACACT+TGG - Chr1:80061797-80061816 MsG0180004590.01.T01:CDS 50.0%
GTGACACTTGGACAAGCTCA+AGG - Chr1:80061809-80061828 MsG0180004590.01.T01:CDS 50.0%
TGAGAGGTCCAAGACAGAAC+AGG + Chr1:80060414-80060433 None:intergenic 50.0%
TGGCTAAGAAGAAAGCGGCT+TGG - Chr1:80061056-80061075 MsG0180004590.01.T01:intron 50.0%
TTATTGCGATGCAGAGGGCT+GGG - Chr1:80060942-80060961 MsG0180004590.01.T01:CDS 50.0%
TTCTGTCTTGGACCTCTCAC+CGG - Chr1:80060415-80060434 MsG0180004590.01.T01:three_prime_UTR 50.0%
! AAGCAATTGGTGGATGTGCG+CGG - Chr1:80060758-80060777 MsG0180004590.01.T01:CDS 50.0%
! ACGATGGAGGAGGTTTTCCA+AGG - Chr1:80060650-80060669 MsG0180004590.01.T01:CDS 50.0%
! GGCAGAAGCACAGAAAGCAT+CGG - Chr1:80061709-80061728 MsG0180004590.01.T01:CDS 50.0%
! TCTTGCTGCTTCCATGACGA+TGG - Chr1:80060634-80060653 MsG0180004590.01.T01:CDS 50.0%
!! CGATAATGGTGAAGGTGCCA+TGG - Chr1:80061994-80062013 MsG0180004590.01.T01:CDS 50.0%
!! GAAGAAGGCTGTGGCTCTAA+GGG - Chr1:80061111-80061130 MsG0180004590.01.T01:intron 50.0%
AAGACTCCCATCCAAGCAGG+TGG + Chr1:80062097-80062116 None:intergenic 55.0%
CGGAGATGTTACCACCTGCT+TGG - Chr1:80062083-80062102 MsG0180004590.01.T01:CDS 55.0%
CTCCCCGCTGTCTTCACTAT+CGG - Chr1:80060491-80060510 MsG0180004590.01.T01:three_prime_UTR 55.0%
GACCGATAGTGAAGACAGCG+GGG + Chr1:80060496-80060515 None:intergenic 55.0%
GGACCGATAGTGAAGACAGC+GGG + Chr1:80060497-80060516 None:intergenic 55.0%
GTTATTGCGATGCAGAGGGC+TGG - Chr1:80060941-80060960 MsG0180004590.01.T01:CDS 55.0%
TATTGCGATGCAGAGGGCTG+GGG - Chr1:80060943-80060962 MsG0180004590.01.T01:CDS 55.0%
TGCTTCCATGACGATGGAGG+AGG - Chr1:80060640-80060659 MsG0180004590.01.T01:CDS 55.0%
! ACTAACGGTGGCGGCGATAA+TGG - Chr1:80061980-80061999 MsG0180004590.01.T01:CDS 55.0%
! CGAAGAAGGCTGTGGCTCTA+AGG - Chr1:80061110-80061129 MsG0180004590.01.T01:intron 55.0%
! GAAGAAAGCGGCTTGGACCA+AGG - Chr1:80061063-80061082 MsG0180004590.01.T01:intron 55.0%
! TGCTGCTTCCATGACGATGG+AGG - Chr1:80060637-80060656 MsG0180004590.01.T01:CDS 55.0%
AGTCGAGGCGAAGAAGGCTG+TGG - Chr1:80061102-80061121 MsG0180004590.01.T01:intron 60.0%
GGCAAGACTCCCATCCAAGC+AGG + Chr1:80062100-80062119 None:intergenic 60.0%
GGCTGAAGTCGAGGCGAAGA+AGG - Chr1:80061096-80061115 MsG0180004590.01.T01:intron 60.0%
! GAAAGCGGCTTGGACCAAGG+CGG - Chr1:80061066-80061085 MsG0180004590.01.T01:intron 60.0%
! GGTGGCGGCGATAATGGTGA+AGG - Chr1:80061986-80062005 MsG0180004590.01.T01:CDS 60.0%
GCTGGGGAGGGTGACAAGGA+GGG - Chr1:80060959-80060978 MsG0180004590.01.T01:CDS 65.0%
GGCTCAGGTGGCTGAAGTCG+AGG - Chr1:80061087-80061106 MsG0180004590.01.T01:intron 65.0%
TTCAGCCACCTGAGCCGCCT+TGG + Chr1:80061083-80061102 None:intergenic 65.0%
TTGGACCAAGGCGGCTCAGG+TGG - Chr1:80061075-80061094 MsG0180004590.01.T01:intron 65.0%
GAGGGCTGGGGAGGGTGACA+AGG - Chr1:80060955-80060974 MsG0180004590.01.T01:CDS 70.0%
GCGATGCAGAGGGCTGGGGA+GGG - Chr1:80060947-80060966 MsG0180004590.01.T01:CDS 70.0%
GGCTGGGGAGGGTGACAAGG+AGG - Chr1:80060958-80060977 MsG0180004590.01.T01:CDS 70.0%
GGCTTGGACCAAGGCGGCTC+AGG - Chr1:80061072-80061091 MsG0180004590.01.T01:intron 70.0%
TGCGATGCAGAGGGCTGGGG+AGG - Chr1:80060946-80060965 MsG0180004590.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 80060209 80062453 80060209 ID=MsG0180004590.01;Name=MsG0180004590.01
Chr1 mRNA 80060209 80062453 80060209 ID=MsG0180004590.01.T01;Parent=MsG0180004590.01;Name=MsG0180004590.01.T01;_AED=0.26;_eAED=0.26;_QI=96|1|1|1|1|1|2|318|477
Chr1 exon 80060209 80061027 80060209 ID=MsG0180004590.01.T01:exon:45763;Parent=MsG0180004590.01.T01
Chr1 exon 80061425 80062453 80061425 ID=MsG0180004590.01.T01:exon:45762;Parent=MsG0180004590.01.T01
Chr1 five_prime_UTR 80062358 80062453 80062358 ID=MsG0180004590.01.T01:five_prime_utr;Parent=MsG0180004590.01.T01
Chr1 CDS 80061425 80062357 80061425 ID=MsG0180004590.01.T01:cds;Parent=MsG0180004590.01.T01
Chr1 CDS 80060527 80061027 80060527 ID=MsG0180004590.01.T01:cds;Parent=MsG0180004590.01.T01
Chr1 three_prime_UTR 80060209 80060526 80060209 ID=MsG0180004590.01.T01:three_prime_utr;Parent=MsG0180004590.01.T01
Gene Sequence

>MsG0180004590.01.T01

ATGAAAATTTACCGGGTTGCAAAAGCATCAGAATACCTTGTGATAACAGGTATTTTGATCAAAGACATAAAACTCGCAAAGAAAGCATGGATTATTCCCGGTCAATCCTGTTCTGTCTTGGACCTCTCACCGGTAAACTACACCTTCGAAGTTCAAGCTATGAGTGCCGAGAAACTTCCTTTTATTCTCCCCGCTGTCTTCACTATCGGTCCTCGTGTAGATGATAAGGAAAGCCTTCTCAAATACGCCAAACTTATTTCACCGCATGCTAGACACTCCAATCATGTTAATGAACTTGTTCAAGGAATCATCGAAGGCGAGACACGTGTTCTTGCTGCTTCCATGACGATGGAGGAGGTTTTCCAAGGAACAAAACAGTTCAAACAAGAAGTGTTCGACAAAGTTCAACTTGAGTTGAATCAATTCGGATTATTGATTTACAATGCGAATGTTAAGCAATTGGTGGATGTGCGCGGTCATGAGTATTTCTCATATTTGGGTCAAAAGACGCAGATGGAAGCCAAGAATCAAGCTAGGGTTGACGTGGCAGAAGCTAAGATGAAAGGTGAAATTGGTTCAAAATTGAGGGAAGGACAAACGCTGCAGAATGCAGCGAAAATTGATGCAGAGACGAAGGTTATTGCGATGCAGAGGGCTGGGGAGGGTGACAAGGAGGGAATTAAAGTGAGGACTGAAGTGAAAGTTTTTGAGAATCAAAGAGAGGCTGAAGTGGCTCAAGCTAATTCTGAGTTGGCTAAGAAGAAAGCGGCTTGGACCAAGGCGGCTCAGGTGGCTGAAGTCGAGGCGAAGAAGGCTGTGGCTCTAAGGGAAGCTGAGTTACAAGGTGAAGTTGAGAGGATGAATGCTTTGACTACTACTGAAAAGCTTAAAGCAGAGTTCCTTAGCAAAGCTAGTGTTCAATATGAAACCAAGGTACAAGAAGCAAACTGGGAGCTGTACAAAAAACAGAAAGAGGCTGAAGCAATTCTGTATGAGAAGAAGGCAGAGGCAGAAGCACAGAAAGCATCGGCAGATGCAACATTTTATGCTAGCAAACAAGCAGCTGAAGCAGAGCTTTATGCAAAGAAAAAGGAGGCAGAGGGAATTGTGACACTTGGACAAGCTCAAGGGGTGTATGTAAGTACACTTCTTAATGCACTTGGAAACAACTACACAGCTGTAAGGGATTACTTGATGATAAATGGTGGCATGTTTCAAGAGATTGCTAAGATTAATGCTGAGGCAATACGCGGACTTGAGCCTAAGATTAGTATTTGGACTAACGGTGGCGGCGATAATGGTGAAGGTGCCATGGGTATGAAAGAAGTTGCTGGTGTCTACAAGATGTTGCCACCTTTGTTTAAGACCGTCCATGAGCAAACGGAGATGTTACCACCTGCTTGGATGGGAGTCTTGCCTGACAAAAACTCTTAA
Protein sequence

>MsG0180004590.01.T01

MKIYRVAKASEYLVITGILIKDIKLAKKAWIIPGQSCSVLDLSPVNYTFEVQAMSAEKLPFILPAVFTIGPRVDDKESLLKYAKLISPHARHSNHVNELVQGIIEGETRVLAASMTMEEVFQGTKQFKQEVFDKVQLELNQFGLLIYNANVKQLVDVRGHEYFSYLGQKTQMEAKNQARVDVAEAKMKGEIGSKLREGQTLQNAAKIDAETKVIAMQRAGEGDKEGIKVRTEVKVFENQREAEVAQANSELAKKKAAWTKAAQVAEVEAKKAVALREAELQGEVERMNALTTTEKLKAEFLSKASVQYETKVQEANWELYKKQKEAEAILYEKKAEAEAQKASADATFYASKQAAEAELYAKKKEAEGIVTLGQAQGVYVSTLLNALGNNYTAVRDYLMINGGMFQEIAKINAEAIRGLEPKISIWTNGGGDNGEGAMGMKEVAGVYKMLPPLFKTVHEQTEMLPPAWMGVLPDKNS*