AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005055.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005055.01.T01 MTR_3g106485 91.004 478 12 3 1 451 1 474 0.0 863
MsG0180005055.01.T01 MTR_3g106480 88.912 478 26 1 1 451 1 478 0.0 855
MsG0180005055.01.T01 MTR_3g106420 88.075 478 30 1 1 451 3 480 0.0 825
MsG0180005055.01.T01 MTR_1g115145 85.714 476 40 4 1 451 1 473 0.0 792
MsG0180005055.01.T01 MTR_1g073400 86.192 478 35 2 1 451 1 474 0.0 781
MsG0180005055.01.T01 MTR_1g083190 84.937 478 37 2 1 451 3 472 0.0 778
MsG0180005055.01.T01 MTR_1g071130 84.061 458 57 4 1 451 1 449 0.0 773
MsG0180005055.01.T01 MTR_3g106430 85.954 477 36 3 1 450 1 473 0.0 762
MsG0180005055.01.T01 MTR_3g106470 84.000 450 43 3 1 450 32 452 0.0 719
MsG0180005055.01.T01 MTR_8g063830 75.699 465 45 6 1 437 1 425 0.0 614
MsG0180005055.01.T01 MTR_3g065540 63.478 115 15 1 40 154 14 101 6.13e-39 136
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005055.01.T01 AT5G25260 70.464 474 101 3 1 447 1 462 0.0 682
MsG0180005055.01.T01 AT5G25250 69.556 473 103 3 1 446 1 459 0.0 661
MsG0180005055.01.T01 AT5G64870 66.737 472 119 2 2 446 3 463 0.0 605

Find 106 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTCCAAGCCGCTTTCTTCT+TGG 0.239260 1:-86301286 None:intergenic
TCACGAGTATTTCTCGTATC+TGG 0.250443 1:+86301008 MsG0180005055.01.T01:CDS
TTGAACCTAAGATTAGTATT+TGG 0.251111 1:+86302822 MsG0180005055.01.T01:CDS
CAACTTGAGTTGAATCAATT+CGG 0.284832 1:+86300937 MsG0180005055.01.T01:CDS
AGGGATTACTTGATGATAAA+TGG 0.307216 1:+86302749 MsG0180005055.01.T01:CDS
AACAAGTTCATTAACATGAT+TGG 0.311801 1:-86300809 None:intergenic
CGAAGAAGGCTGTGGCTCTA+AGG 0.317541 1:+86301337 MsG0180005055.01.T01:CDS
TGTAGGTACAAGAGGCTAAC+TGG 0.318803 1:+86302495 MsG0180005055.01.T01:intron
GCTAAGATGAAAGGTGAAAT+TGG 0.319472 1:+86301084 MsG0180005055.01.T01:CDS
CATGTTGCCTCCTGCTTGGA+TGG 0.319807 1:+86302961 MsG0180005055.01.T01:CDS
TTAGTCCAAATACTAATCTT+AGG 0.325227 1:-86302827 None:intergenic
GTGAAATTGGTTCGAAATTG+AGG 0.356446 1:+86301097 MsG0180005055.01.T01:CDS
TCAGAATATCTTGCGATAAC+TGG 0.374224 1:+86300559 MsG0180005055.01.T01:CDS
CAATGCGAATGTTAAGCAAT+TGG 0.388576 1:+86300972 MsG0180005055.01.T01:CDS
GGCTTGGACCAAGGCGGCTC+AGG 0.413477 1:+86301299 MsG0180005055.01.T01:CDS
CATGTTAATGAACTTGTTCA+AGG 0.419850 1:+86300814 MsG0180005055.01.T01:CDS
GGGAATAACTGAAGGTGCTA+TGG 0.428010 1:+86302868 MsG0180005055.01.T01:CDS
TTCAGCCACCTGAGCCGCCT+TGG 0.429757 1:-86301307 None:intergenic
TGGACTAACGGTGGTGATAA+TGG 0.448931 1:+86302842 MsG0180005055.01.T01:CDS
ATCATGGGGTGAAATAAGTT+TGG 0.453058 1:-86300779 None:intergenic
AACTTGAGTTGAATCAATTC+GGG 0.454308 1:+86300938 MsG0180005055.01.T01:CDS
CTGGCATGTTGCCTCCTGCT+TGG 0.459012 1:+86302957 MsG0180005055.01.T01:CDS
TGAGAATCAGAGAGAGGCTG+AGG 0.460493 1:+86301239 MsG0180005055.01.T01:intron
AGCAATTCTGTATGAGAAGA+AGG 0.462456 1:+86302547 MsG0180005055.01.T01:CDS
CTCCCTGCCGTCTTCACTAT+CGG 0.465580 1:+86300718 MsG0180005055.01.T01:CDS
GGCTGAAGTTGAGGCGAAGA+AGG 0.470534 1:+86301323 MsG0180005055.01.T01:CDS
AAGACTGTCCATGAGCAAAC+TGG 0.472099 1:+86302938 MsG0180005055.01.T01:CDS
TTGGACCAAGGCGGCTCAGG+TGG 0.476684 1:+86301302 MsG0180005055.01.T01:CDS
GCAGAGGGGATTGCGACACT+TGG 0.477405 1:+86302662 MsG0180005055.01.T01:CDS
CACGAGTATTTCTCGTATCT+GGG 0.484574 1:+86301009 MsG0180005055.01.T01:CDS
TGAAATTGGTTCGAAATTGA+GGG 0.484739 1:+86301098 MsG0180005055.01.T01:CDS
AAACCAAAATCAACATGTAC+CGG 0.488241 1:+86300524 None:intergenic
ATGTTGCCTCCTGCTTGGAT+GGG 0.491718 1:+86302962 MsG0180005055.01.T01:CDS
CATGATTGGAGTGTCTATCA+TGG 0.495785 1:-86300795 None:intergenic
TGCTTCCATGACCATGGAGG+AGG 0.495947 1:+86300867 MsG0180005055.01.T01:CDS
CTTGTTCAAGGAATCATTGA+AGG 0.497958 1:+86300826 MsG0180005055.01.T01:CDS
AACTAAGAAGGTATAGTTCA+CGG 0.503038 1:-86300662 None:intergenic
TTCAAGTCCGCGTACCGCCT+CGG 0.504382 1:-86302804 None:intergenic
GAAAACCTCCTCCATGGTCA+TGG 0.505571 1:-86300872 None:intergenic
TGGAAGCAGCGAATCAAGCT+AGG 0.506083 1:+86301046 MsG0180005055.01.T01:CDS
GAAACAACTACACAGCAGTA+AGG 0.507741 1:+86302729 MsG0180005055.01.T01:CDS
GGACCGATAGTGAAGACGGC+AGG 0.514852 1:-86300721 None:intergenic
AAGACGGCAGGGAGAACGAA+AGG 0.516090 1:-86300709 None:intergenic
GGAATAACTGAAGGTGCTAT+GGG 0.526339 1:+86302869 MsG0180005055.01.T01:CDS
TAAGTTTGGCGTATTTGAGT+AGG 0.526369 1:-86300765 None:intergenic
AAGCAATTGGTGGATGTGCC+TGG 0.526695 1:+86300985 MsG0180005055.01.T01:CDS
ACTCATAGCTTGAACTAAGA+AGG 0.526930 1:-86300674 None:intergenic
GCAACATGCCAGTTTGCTCA+TGG 0.527154 1:-86302946 None:intergenic
TGCTGAGGCTAATTCTGAGT+TGG 0.528368 1:+86301263 MsG0180005055.01.T01:CDS
CTAAGGGAAGCTGAGTTACA+AGG 0.536670 1:+86301354 MsG0180005055.01.T01:CDS
GGCTCAGGTGGCTGAAGTTG+AGG 0.543832 1:+86301314 MsG0180005055.01.T01:CDS
GAAGAAGGCTGTGGCTCTAA+GGG 0.545005 1:+86301338 MsG0180005055.01.T01:CDS
GTAGGTACAAGAGGCTAACT+GGG 0.550232 1:+86302496 MsG0180005055.01.T01:intron
GATAAGGATCCCATCCAAGC+AGG 0.550974 1:-86302971 None:intergenic
CTGGATGCTGTAGGTACAAG+AGG 0.556449 1:+86302487 MsG0180005055.01.T01:intron
GAAGAAAGCGGCTTGGACCA+AGG 0.558168 1:+86301290 MsG0180005055.01.T01:CDS
AGTTGAGGCGAAGAAGGCTG+TGG 0.558891 1:+86301329 MsG0180005055.01.T01:CDS
TGCGAATGTTAAGCAATTGG+TGG 0.563120 1:+86300975 MsG0180005055.01.T01:CDS
ATGGGTATGAAAGAAGTTGC+TGG 0.569410 1:+86302887 MsG0180005055.01.T01:CDS
AGTACACTTCTCAATGCACT+TGG 0.570595 1:+86302707 MsG0180005055.01.T01:CDS
TCCTCTGAAAACCTCCTCCA+TGG 0.572262 1:-86300878 None:intergenic
CAGAGGGGATTGCGACACTT+GGG 0.572412 1:+86302663 MsG0180005055.01.T01:CDS
TATCTTGCGATAACTGGTGT+AGG 0.573302 1:+86300565 MsG0180005055.01.T01:CDS
ACTAAGAAGGTATAGTTCAC+GGG 0.573332 1:-86300661 None:intergenic
AAGATTAGTATTTGGACTAA+CGG 0.575927 1:+86302830 MsG0180005055.01.T01:CDS
GAGAGAGGCTGAGGTTGCTG+AGG 0.577125 1:+86301248 MsG0180005055.01.T01:intron
TCTAGCTGCTTCCATGACCA+TGG 0.581097 1:+86300861 MsG0180005055.01.T01:CDS
GCGACACTTGGGAATGCTCA+AGG 0.583461 1:+86302674 MsG0180005055.01.T01:CDS
TAAAACTCGAAAAGAAAGCA+TGG 0.584686 1:+86300599 MsG0180005055.01.T01:CDS
TGGCCAAGAAGAAAGCGGCT+TGG 0.586771 1:+86301283 MsG0180005055.01.T01:CDS
ATGATTGGAGTGTCTATCAT+GGG 0.587404 1:-86300794 None:intergenic
AGGTATAGTTCACGGGTGAG+AGG 0.596809 1:-86300654 None:intergenic
AACGGTGGTGATAATGGTGG+GGG 0.598797 1:+86302848 MsG0180005055.01.T01:CDS
AAACAACTACACAGCAGTAA+GGG 0.599439 1:+86302730 MsG0180005055.01.T01:CDS
TCTGTATGAGAAGAAGGCAG+AGG 0.600808 1:+86302553 MsG0180005055.01.T01:CDS
GACCGATAGTGAAGACGGCA+GGG 0.602102 1:-86300720 None:intergenic
TACGAGAAATACTCGTGACC+AGG 0.605693 1:-86301003 None:intergenic
TAACGGTGGTGATAATGGTG+GGG 0.609681 1:+86302847 MsG0180005055.01.T01:CDS
GTGGCAGAAGCTAAGATGAA+AGG 0.614706 1:+86301075 MsG0180005055.01.T01:CDS
TCTGGGACAAAAGACGCAGA+TGG 0.620653 1:+86301026 MsG0180005055.01.T01:CDS
CTAACGGTGGTGATAATGGT+GGG 0.630678 1:+86302846 MsG0180005055.01.T01:CDS
ATTGGTTCGAAATTGAGGGA+AGG 0.631232 1:+86301102 MsG0180005055.01.T01:CDS
AGACGGCAGGGAGAACGAAA+GGG 0.631236 1:-86300708 None:intergenic
GGAAGCTGAGTTACAAGGCG+AGG 0.636048 1:+86301359 MsG0180005055.01.T01:CDS
GGAAGCAGCGAATCAAGCTA+GGG 0.637623 1:+86301047 MsG0180005055.01.T01:CDS
ACGAGGACCGATAGTGAAGA+CGG 0.639502 1:-86300725 None:intergenic
AACCAAAATCAACATGTACC+GGG 0.642639 1:+86300525 None:intergenic
GATTGCTAAGATTAATGCCG+AGG 0.643593 1:+86302787 MsG0180005055.01.T01:CDS
TGAGTTGGCCAAGAAGAAAG+CGG 0.645893 1:+86301278 MsG0180005055.01.T01:CDS
TAGTGTTCAGTATGAAACCA+AGG 0.647410 1:+86301443 MsG0180005055.01.T01:CDS
GAAAATTGATGCAGAGACGA+AGG 0.652587 1:+86301146 MsG0180005055.01.T01:CDS
AAGAAAGCATGGATTTACCC+TGG 0.654675 1:+86300610 MsG0180005055.01.T01:CDS
TGGACAGTCTTAAACAGAGG+TGG 0.656626 1:-86302926 None:intergenic
GATTACTTGATGATAAATGG+TGG 0.658453 1:+86302752 MsG0180005055.01.T01:CDS
TCATGGACAGTCTTAAACAG+AGG 0.660499 1:-86302929 None:intergenic
TGCTAAGATTAATGCCGAGG+CGG 0.661554 1:+86302790 MsG0180005055.01.T01:CDS
AAGGATCCCATCCAAGCAGG+AGG 0.669522 1:-86302968 None:intergenic
AGCTGCTTCCATGACCATGG+AGG 0.676991 1:+86300864 MsG0180005055.01.T01:CDS
ATTAATGCCGAGGCGGTACG+CGG 0.680968 1:+86302797 MsG0180005055.01.T01:CDS
GAATCAAGCTAGGGTTGACG+TGG 0.682564 1:+86301056 MsG0180005055.01.T01:CDS
ATTAGTATTTGGACTAACGG+TGG 0.682600 1:+86302833 MsG0180005055.01.T01:CDS
CTTTCATGATCGTCAACACG+AGG 0.688237 1:-86300742 None:intergenic
ACTAACGGTGGTGATAATGG+TGG 0.694563 1:+86302845 MsG0180005055.01.T01:CDS
TTGCTTGCGAGCATAAAACG+TGG 0.719700 1:-86302603 None:intergenic
GAAAGCGGCTTGGACCAAGG+CGG 0.735762 1:+86301293 MsG0180005055.01.T01:CDS
TGATTGGAGTGTCTATCATG+GGG 0.776734 1:-86300793 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAAATATAATTAATAGG+TGG - Chr1:86302242-86302261 None:intergenic 10.0%
!! CATTAAAAAATTAAATCATT+TGG + Chr1:86302429-86302448 MsG0180005055.01.T01:intron 10.0%
!! AAAATTAAATCATTTGGTAA+CGG + Chr1:86302435-86302454 MsG0180005055.01.T01:intron 15.0%
!! AATCACAATAATTTATTACT+TGG - Chr1:86301980-86301999 None:intergenic 15.0%
!! TTAACAAATAATTGCTAAAA+TGG + Chr1:86301822-86301841 MsG0180005055.01.T01:intron 15.0%
!!! TCTTGTTTCATTTATTAATT+AGG + Chr1:86301487-86301506 MsG0180005055.01.T01:intron 15.0%
!! AGAAAAAATTATTCTGTGTT+AGG + Chr1:86302126-86302145 MsG0180005055.01.T01:intron 20.0%
!! TATAAACTTGATCAATCTAA+TGG - Chr1:86301569-86301588 None:intergenic 20.0%
!! TATTAATTAGGTGAATTCTA+GGG + Chr1:86301499-86301518 MsG0180005055.01.T01:intron 20.0%
!! TCATCTTTATAATTAAAACC+AGG - Chr1:86301780-86301799 None:intergenic 20.0%
!! TGCATATTGTTATAAAAAGA+TGG + Chr1:86302332-86302351 MsG0180005055.01.T01:intron 20.0%
!! TTATTAATTAGGTGAATTCT+AGG + Chr1:86301498-86301517 MsG0180005055.01.T01:intron 20.0%
! AACAAGTTCATTAACATGAT+TGG - Chr1:86300812-86300831 None:intergenic 25.0%
! AAGATTAGTATTTGGACTAA+CGG + Chr1:86302830-86302849 MsG0180005055.01.T01:CDS 25.0%
! CTAAGATTGGATTAAATCAA+GGG + Chr1:86301905-86301924 MsG0180005055.01.T01:intron 25.0%
! TAAGATTGGATTAAATCAAG+GGG + Chr1:86301906-86301925 MsG0180005055.01.T01:intron 25.0%
! TTAGTCCAAATACTAATCTT+AGG - Chr1:86302830-86302849 None:intergenic 25.0%
! TTGAACCTAAGATTAGTATT+TGG + Chr1:86302822-86302841 MsG0180005055.01.T01:CDS 25.0%
!! CTTAATTGAAGTATTACTCT+TGG - Chr1:86302278-86302297 None:intergenic 25.0%
!! TCTAAGATTGGATTAAATCA+AGG + Chr1:86301904-86301923 MsG0180005055.01.T01:intron 25.0%
!! TGATGTATTTTCTTTAGCAA+CGG - Chr1:86302025-86302044 None:intergenic 25.0%
AAACCAGGACAATATATTAG+AGG - Chr1:86301765-86301784 None:intergenic 30.0%
AACAAGAAGTTATAGTTGCA+TGG - Chr1:86301474-86301493 None:intergenic 30.0%
AAGATTGGATTAAATCAAGG+GGG + Chr1:86301907-86301926 MsG0180005055.01.T01:intron 30.0%
AGCAAATGATGTATGTTCAA+CGG - Chr1:86302397-86302416 None:intergenic 30.0%
ATCTGATCAAACTTTCATCA+TGG + Chr1:86302362-86302381 MsG0180005055.01.T01:intron 30.0%
ATTCCTCTAATATATTGTCC+TGG + Chr1:86301759-86301778 MsG0180005055.01.T01:intron 30.0%
GAACCATGAATAAAAACCAT+CGG - Chr1:86302183-86302202 None:intergenic 30.0%
TAAAACTCGAAAAGAAAGCA+TGG + Chr1:86300599-86300618 MsG0180005055.01.T01:CDS 30.0%
TAGAGGAATAATCCTCAAAT+AGG - Chr1:86301748-86301767 None:intergenic 30.0%
TGCTAAAATGGATGAAAACA+AGG + Chr1:86301834-86301853 MsG0180005055.01.T01:intron 30.0%
! AACTAAGAAGGTATAGTTCA+CGG - Chr1:86300665-86300684 None:intergenic 30.0%
! AATCCGATGGTTTTTATTCA+TGG + Chr1:86302177-86302196 MsG0180005055.01.T01:intron 30.0%
! AGGGATTACTTGATGATAAA+TGG + Chr1:86302749-86302768 MsG0180005055.01.T01:CDS 30.0%
! CATGTTAATGAACTTGTTCA+AGG + Chr1:86300814-86300833 MsG0180005055.01.T01:CDS 30.0%
! GATTACTTGATGATAAATGG+TGG + Chr1:86302752-86302771 MsG0180005055.01.T01:CDS 30.0%
!! AACTTGAGTTGAATCAATTC+GGG + Chr1:86300938-86300957 MsG0180005055.01.T01:CDS 30.0%
!! CAACTTGAGTTGAATCAATT+CGG + Chr1:86300937-86300956 MsG0180005055.01.T01:CDS 30.0%
!! GGTGTTTATATTTTAAGTGC+TGG + Chr1:86302456-86302475 MsG0180005055.01.T01:intron 30.0%
!! TGAAATTGGTTCGAAATTGA+GGG + Chr1:86301098-86301117 MsG0180005055.01.T01:CDS 30.0%
AAACAACTACACAGCAGTAA+GGG + Chr1:86302730-86302749 MsG0180005055.01.T01:CDS 35.0%
AATCTAATGGTTGTTCCAAG+TGG - Chr1:86301556-86301575 None:intergenic 35.0%
ACTCATAGCTTGAACTAAGA+AGG - Chr1:86300677-86300696 None:intergenic 35.0%
AGAAGCAAGGAATTAAAGTG+AGG + Chr1:86301199-86301218 MsG0180005055.01.T01:intron 35.0%
AGCAATTCTGTATGAGAAGA+AGG + Chr1:86302547-86302566 MsG0180005055.01.T01:CDS 35.0%
ATCATGGGGTGAAATAAGTT+TGG - Chr1:86300782-86300801 None:intergenic 35.0%
ATCCTCAAATAGGATATGTG+AGG - Chr1:86301738-86301757 None:intergenic 35.0%
ATTAGTATTTGGACTAACGG+TGG + Chr1:86302833-86302852 MsG0180005055.01.T01:CDS 35.0%
CAATGCGAATGTTAAGCAAT+TGG + Chr1:86300972-86300991 MsG0180005055.01.T01:CDS 35.0%
CTTGTTCAAGGAATCATTGA+AGG + Chr1:86300826-86300845 MsG0180005055.01.T01:CDS 35.0%
GCTAAGATGAAAGGTGAAAT+TGG + Chr1:86301084-86301103 MsG0180005055.01.T01:CDS 35.0%
GCTATACGCAAAGAAAAAAG+AGG + Chr1:86302640-86302659 MsG0180005055.01.T01:CDS 35.0%
GTTAGGTACATCATAAGATC+TGG + Chr1:86302143-86302162 MsG0180005055.01.T01:intron 35.0%
TAAGTTTGGCGTATTTGAGT+AGG - Chr1:86300768-86300787 None:intergenic 35.0%
TAGTGTTCAGTATGAAACCA+AGG + Chr1:86301443-86301462 MsG0180005055.01.T01:CDS 35.0%
TCAAATAGGATATGTGAGGA+CGG - Chr1:86301734-86301753 None:intergenic 35.0%
TCAGAATATCTTGCGATAAC+TGG + Chr1:86300559-86300578 MsG0180005055.01.T01:CDS 35.0%
TCATAACTGTGCTTAGAAGA+TGG + Chr1:86302049-86302068 MsG0180005055.01.T01:intron 35.0%
TGCTTAGAAGATGGTGATTT+GGG + Chr1:86302058-86302077 MsG0180005055.01.T01:intron 35.0%
! ACTAAGAAGGTATAGTTCAC+GGG - Chr1:86300664-86300683 None:intergenic 35.0%
! ATGATTGGAGTGTCTATCAT+GGG - Chr1:86300797-86300816 None:intergenic 35.0%
! TCAAGAGCTTTTGTCAGATA+AGG - Chr1:86302990-86303009 None:intergenic 35.0%
!! AGTTTTTGAGAATCAGAGAG+AGG + Chr1:86301233-86301252 MsG0180005055.01.T01:intron 35.0%
!! GTGAAATTGGTTCGAAATTG+AGG + Chr1:86301097-86301116 MsG0180005055.01.T01:CDS 35.0%
!! TTAAGTGCTGGTTGAAATTC+TGG + Chr1:86302468-86302487 MsG0180005055.01.T01:intron 35.0%
AGTACACTTCTCAATGCACT+TGG + Chr1:86302707-86302726 MsG0180005055.01.T01:CDS 40.0%
ATGGGTATGAAAGAAGTTGC+TGG + Chr1:86302887-86302906 MsG0180005055.01.T01:CDS 40.0%
CAAAGAAAAAAGAGGCAGAG+GGG + Chr1:86302648-86302667 MsG0180005055.01.T01:CDS 40.0%
CACGAGTATTTCTCGTATCT+GGG + Chr1:86301009-86301028 MsG0180005055.01.T01:CDS 40.0%
GAAAATTGATGCAGAGACGA+AGG + Chr1:86301146-86301165 MsG0180005055.01.T01:CDS 40.0%
GAAACAACTACACAGCAGTA+AGG + Chr1:86302729-86302748 MsG0180005055.01.T01:CDS 40.0%
GAAGGTTATTGCGATGTAGA+GGG + Chr1:86301164-86301183 MsG0180005055.01.T01:intron 40.0%
GATTGCTAAGATTAATGCCG+AGG + Chr1:86302787-86302806 MsG0180005055.01.T01:CDS 40.0%
GCAAAGAAAAAAGAGGCAGA+GGG + Chr1:86302647-86302666 MsG0180005055.01.T01:CDS 40.0%
GGTACATCATAAGATCTGGT+AGG + Chr1:86302147-86302166 MsG0180005055.01.T01:intron 40.0%
GGTGCGATCATGAACTAAAT+GGG - Chr1:86301665-86301684 None:intergenic 40.0%
GTCCTCACATATCCTATTTG+AGG + Chr1:86301733-86301752 MsG0180005055.01.T01:intron 40.0%
GTGCTTAGAAGATGGTGATT+TGG + Chr1:86302057-86302076 MsG0180005055.01.T01:intron 40.0%
GTTGAAATTCTGGATGCTGT+AGG + Chr1:86302478-86302497 MsG0180005055.01.T01:intron 40.0%
TATCTTGCGATAACTGGTGT+AGG + Chr1:86300565-86300584 MsG0180005055.01.T01:CDS 40.0%
TCACGAGTATTTCTCGTATC+TGG + Chr1:86301008-86301027 MsG0180005055.01.T01:CDS 40.0%
TCATGGACAGTCTTAAACAG+AGG - Chr1:86302932-86302951 None:intergenic 40.0%
TGCGAATGTTAAGCAATTGG+TGG + Chr1:86300975-86300994 MsG0180005055.01.T01:CDS 40.0%
TGGTGCGATCATGAACTAAA+TGG - Chr1:86301666-86301685 None:intergenic 40.0%
TTACCCTGGTCAAAAATGCA+CGG + Chr1:86300624-86300643 MsG0180005055.01.T01:CDS 40.0%
TTCACCATGAACCACTTAGT+TGG + Chr1:86302200-86302219 MsG0180005055.01.T01:intron 40.0%
! AAGAAAGCATGGATTTACCC+TGG + Chr1:86300610-86300629 MsG0180005055.01.T01:CDS 40.0%
! ATAGTTGCATGGACTAACCT+TGG - Chr1:86301463-86301482 None:intergenic 40.0%
! ATTCTGATGCTTTTGCTACC+CGG - Chr1:86300546-86300565 None:intergenic 40.0%
! CATGATTGGAGTGTCTATCA+TGG - Chr1:86300798-86300817 None:intergenic 40.0%
! GAGAACGAAAGGGAGTTTTT+CGG - Chr1:86300701-86300720 None:intergenic 40.0%
! TGATTGGAGTGTCTATCATG+GGG - Chr1:86300796-86300815 None:intergenic 40.0%
!! ATTAGGTGAATTCTAGGGTG+AGG + Chr1:86301504-86301523 MsG0180005055.01.T01:intron 40.0%
!! ATTGGTTCGAAATTGAGGGA+AGG + Chr1:86301102-86301121 MsG0180005055.01.T01:CDS 40.0%
!! CGTGAGAAGTGTTCTAAGAT+TGG + Chr1:86301892-86301911 MsG0180005055.01.T01:intron 40.0%
!! GGAATAACTGAAGGTGCTAT+GGG + Chr1:86302869-86302888 MsG0180005055.01.T01:CDS 40.0%
!! TTAGGTGAATTCTAGGGTGA+GGG + Chr1:86301505-86301524 MsG0180005055.01.T01:intron 40.0%
AAGACTGTCCATGAGCAAAC+TGG + Chr1:86302938-86302957 MsG0180005055.01.T01:CDS 45.0%
ACCACCAACTAAGTGGTTCA+TGG - Chr1:86302207-86302226 None:intergenic 45.0%
ACCATGAACCACTTAGTTGG+TGG + Chr1:86302203-86302222 MsG0180005055.01.T01:intron 45.0%
AGAGTGAACCACCAACTAAG+TGG - Chr1:86302214-86302233 None:intergenic 45.0%
CGAAGGTTATTGCGATGTAG+AGG + Chr1:86301163-86301182 MsG0180005055.01.T01:intron 45.0%
CGCAAAGAAAAAAGAGGCAG+AGG + Chr1:86302646-86302665 MsG0180005055.01.T01:CDS 45.0%
CTAAGGGAAGCTGAGTTACA+AGG + Chr1:86301354-86301373 MsG0180005055.01.T01:CDS 45.0%
CTTTCATGATCGTCAACACG+AGG - Chr1:86300745-86300764 None:intergenic 45.0%
GCGATCATGAACTAAATGGG+AGG - Chr1:86301662-86301681 None:intergenic 45.0%
GGTAGGACAACATAATCCGA+TGG + Chr1:86302164-86302183 MsG0180005055.01.T01:intron 45.0%
GTAGGTACAAGAGGCTAACT+GGG + Chr1:86302496-86302515 MsG0180005055.01.T01:intron 45.0%
GTGGCAGAAGCTAAGATGAA+AGG + Chr1:86301075-86301094 MsG0180005055.01.T01:CDS 45.0%
TACGAGAAATACTCGTGACC+AGG - Chr1:86301006-86301025 None:intergenic 45.0%
TCTGTATGAGAAGAAGGCAG+AGG + Chr1:86302553-86302572 MsG0180005055.01.T01:CDS 45.0%
TGCTAAGATTAATGCCGAGG+CGG + Chr1:86302790-86302809 MsG0180005055.01.T01:CDS 45.0%
TGCTGAGGCTAATTCTGAGT+TGG + Chr1:86301263-86301282 MsG0180005055.01.T01:CDS 45.0%
TGGACAGTCTTAAACAGAGG+TGG - Chr1:86302929-86302948 None:intergenic 45.0%
TGGACTAACGGTGGTGATAA+TGG + Chr1:86302842-86302861 MsG0180005055.01.T01:CDS 45.0%
TGTAGGTACAAGAGGCTAAC+TGG + Chr1:86302495-86302514 MsG0180005055.01.T01:intron 45.0%
TTGCTTGCGAGCATAAAACG+TGG - Chr1:86302606-86302625 None:intergenic 45.0%
! AAGACCGTGCATTTTTGACC+AGG - Chr1:86300631-86300650 None:intergenic 45.0%
! AATGGTGGGGGAATAACTGA+AGG + Chr1:86302860-86302879 MsG0180005055.01.T01:CDS 45.0%
! AGACCGTGCATTTTTGACCA+GGG - Chr1:86300630-86300649 None:intergenic 45.0%
! GATGGTGATTTGGGAGTTGA+TGG + Chr1:86302067-86302086 MsG0180005055.01.T01:intron 45.0%
! TCGTGGATAGCAGTTTTCCT+GGG - Chr1:86301701-86301720 None:intergenic 45.0%
! TGAGTTGGCCAAGAAGAAAG+CGG + Chr1:86301278-86301297 MsG0180005055.01.T01:CDS 45.0%
! TTCGTGGATAGCAGTTTTCC+TGG - Chr1:86301702-86301721 None:intergenic 45.0%
!! ACTAACGGTGGTGATAATGG+TGG + Chr1:86302845-86302864 MsG0180005055.01.T01:CDS 45.0%
!! CTAACGGTGGTGATAATGGT+GGG + Chr1:86302846-86302865 MsG0180005055.01.T01:CDS 45.0%
!! GGGAATAACTGAAGGTGCTA+TGG + Chr1:86302868-86302887 MsG0180005055.01.T01:CDS 45.0%
!! TAACGGTGGTGATAATGGTG+GGG + Chr1:86302847-86302866 MsG0180005055.01.T01:CDS 45.0%
!! ACAATTTAAATATAATTAAT+AGG - Chr1:86302245-86302264 None:intergenic 5.0%
ACGAGGACCGATAGTGAAGA+CGG - Chr1:86300728-86300747 None:intergenic 50.0%
AGGTATAGTTCACGGGTGAG+AGG - Chr1:86300657-86300676 None:intergenic 50.0%
ATGTTGCCTCCTGCTTGGAT+GGG + Chr1:86302962-86302981 MsG0180005055.01.T01:CDS 50.0%
CTGGATGCTGTAGGTACAAG+AGG + Chr1:86302487-86302506 MsG0180005055.01.T01:intron 50.0%
GAAAACCTCCTCCATGGTCA+TGG - Chr1:86300875-86300894 None:intergenic 50.0%
GAATCAAGCTAGGGTTGACG+TGG + Chr1:86301056-86301075 MsG0180005055.01.T01:CDS 50.0%
GATAAGGATCCCATCCAAGC+AGG - Chr1:86302974-86302993 None:intergenic 50.0%
GCTGGTGAAAGTGAGAAGCA+AGG + Chr1:86301186-86301205 MsG0180005055.01.T01:intron 50.0%
GGAAGCAGCGAATCAAGCTA+GGG + Chr1:86301047-86301066 MsG0180005055.01.T01:CDS 50.0%
GGCTCTATTAAGTCCCTCAC+CGG + Chr1:86301526-86301545 MsG0180005055.01.T01:intron 50.0%
GTTATTGCGATGTAGAGGGC+TGG + Chr1:86301168-86301187 MsG0180005055.01.T01:intron 50.0%
TCTAGCTGCTTCCATGACCA+TGG + Chr1:86300861-86300880 MsG0180005055.01.T01:CDS 50.0%
TCTATTAAGTCCCTCACCGG+TGG + Chr1:86301529-86301548 MsG0180005055.01.T01:intron 50.0%
TGAGAATCAGAGAGAGGCTG+AGG + Chr1:86301239-86301258 MsG0180005055.01.T01:intron 50.0%
TGGAAGCAGCGAATCAAGCT+AGG + Chr1:86301046-86301065 MsG0180005055.01.T01:CDS 50.0%
! AAGCAATTGGTGGATGTGCC+TGG + Chr1:86300985-86301004 MsG0180005055.01.T01:CDS 50.0%
! ACCATGGAGGAGGTTTTCAG+AGG + Chr1:86300877-86300896 MsG0180005055.01.T01:CDS 50.0%
! GCAACATGCCAGTTTGCTCA+TGG - Chr1:86302949-86302968 None:intergenic 50.0%
! GGCAGAAGCGCAAAAAGCAT+TGG + Chr1:86302574-86302593 MsG0180005055.01.T01:CDS 50.0%
! TCCTCTGAAAACCTCCTCCA+TGG - Chr1:86300881-86300900 None:intergenic 50.0%
! TCTGGGACAAAAGACGCAGA+TGG + Chr1:86301026-86301045 MsG0180005055.01.T01:CDS 50.0%
! TTCCTGGGCACTGCAAACTT+TGG - Chr1:86301686-86301705 None:intergenic 50.0%
!! AACGGTGGTGATAATGGTGG+GGG + Chr1:86302848-86302867 MsG0180005055.01.T01:CDS 50.0%
!! GAAGAAGGCTGTGGCTCTAA+GGG + Chr1:86301338-86301357 MsG0180005055.01.T01:CDS 50.0%
!! GGTGATTTGGGAGTTGATGG+TGG + Chr1:86302070-86302089 MsG0180005055.01.T01:intron 50.0%
AAGACGGCAGGGAGAACGAA+AGG - Chr1:86300712-86300731 None:intergenic 55.0%
AAGGATCCCATCCAAGCAGG+AGG - Chr1:86302971-86302990 None:intergenic 55.0%
AGACGGCAGGGAGAACGAAA+GGG - Chr1:86300711-86300730 None:intergenic 55.0%
AGCTGCTTCCATGACCATGG+AGG + Chr1:86300864-86300883 MsG0180005055.01.T01:CDS 55.0%
AGGATATGTGAGGACGGCAG+TGG - Chr1:86301728-86301747 None:intergenic 55.0%
AGTTGAGGCGAAGAAGGCTG+TGG + Chr1:86301329-86301348 MsG0180005055.01.T01:CDS 55.0%
ATATGTGAGGACGGCAGTGG+AGG - Chr1:86301725-86301744 None:intergenic 55.0%
ATTAATGCCGAGGCGGTACG+CGG + Chr1:86302797-86302816 MsG0180005055.01.T01:CDS 55.0%
CACCAAAGTTTGCAGTGCCC+AGG + Chr1:86301681-86301700 MsG0180005055.01.T01:intron 55.0%
CAGAGGGGATTGCGACACTT+GGG + Chr1:86302663-86302682 MsG0180005055.01.T01:CDS 55.0%
CATGTTGCCTCCTGCTTGGA+TGG + Chr1:86302961-86302980 MsG0180005055.01.T01:CDS 55.0%
CTCCCTGCCGTCTTCACTAT+CGG + Chr1:86300718-86300737 MsG0180005055.01.T01:CDS 55.0%
GACCGATAGTGAAGACGGCA+GGG - Chr1:86300723-86300742 None:intergenic 55.0%
GCGACACTTGGGAATGCTCA+AGG + Chr1:86302674-86302693 MsG0180005055.01.T01:CDS 55.0%
GGAAGCTGAGTTACAAGGCG+AGG + Chr1:86301359-86301378 MsG0180005055.01.T01:CDS 55.0%
GGCTGAAGTTGAGGCGAAGA+AGG + Chr1:86301323-86301342 MsG0180005055.01.T01:CDS 55.0%
GGTTGTTCCAAGTGGTCCAC+CGG - Chr1:86301548-86301567 None:intergenic 55.0%
TGCTTCCATGACCATGGAGG+AGG + Chr1:86300867-86300886 MsG0180005055.01.T01:CDS 55.0%
TGGCCAAGAAGAAAGCGGCT+TGG + Chr1:86301283-86301302 MsG0180005055.01.T01:CDS 55.0%
! CGAAGAAGGCTGTGGCTCTA+AGG + Chr1:86301337-86301356 MsG0180005055.01.T01:CDS 55.0%
! GAAGAAAGCGGCTTGGACCA+AGG + Chr1:86301290-86301309 MsG0180005055.01.T01:CDS 55.0%
! GGTCCAAGCCGCTTTCTTCT+TGG - Chr1:86301289-86301308 None:intergenic 55.0%
CTGGCATGTTGCCTCCTGCT+TGG + Chr1:86302957-86302976 MsG0180005055.01.T01:CDS 60.0%
GAGAGAGGCTGAGGTTGCTG+AGG + Chr1:86301248-86301267 MsG0180005055.01.T01:intron 60.0%
GCAGAGGGGATTGCGACACT+TGG + Chr1:86302662-86302681 MsG0180005055.01.T01:CDS 60.0%
GGACCGATAGTGAAGACGGC+AGG - Chr1:86300724-86300743 None:intergenic 60.0%
GGCTCAGGTGGCTGAAGTTG+AGG + Chr1:86301314-86301333 MsG0180005055.01.T01:CDS 60.0%
TCCAAGTGGTCCACCGGTGA+GGG - Chr1:86301542-86301561 None:intergenic 60.0%
TTCAAGTCCGCGTACCGCCT+CGG - Chr1:86302807-86302826 None:intergenic 60.0%
TTCCAAGTGGTCCACCGGTG+AGG - Chr1:86301543-86301562 None:intergenic 60.0%
! GAAAGCGGCTTGGACCAAGG+CGG + Chr1:86301293-86301312 MsG0180005055.01.T01:CDS 60.0%
AGGACGGCAGTGGAGGTTCG+TGG - Chr1:86301718-86301737 None:intergenic 65.0%
TCCCTCACCGGTGGACCACT+TGG + Chr1:86301538-86301557 MsG0180005055.01.T01:intron 65.0%
TTCAGCCACCTGAGCCGCCT+TGG - Chr1:86301310-86301329 None:intergenic 65.0%
TTGGACCAAGGCGGCTCAGG+TGG + Chr1:86301302-86301321 MsG0180005055.01.T01:CDS 65.0%
GGCTTGGACCAAGGCGGCTC+AGG + Chr1:86301299-86301318 MsG0180005055.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 86300538 86303009 86300538 ID=MsG0180005055.01;Name=MsG0180005055.01
Chr1 mRNA 86300538 86303009 86300538 ID=MsG0180005055.01.T01;Parent=MsG0180005055.01;Name=MsG0180005055.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|1|3|0|451
Chr1 exon 86300538 86301167 86300538 ID=MsG0180005055.01.T01:exon:28389;Parent=MsG0180005055.01.T01
Chr1 exon 86301249 86301464 86301249 ID=MsG0180005055.01.T01:exon:28390;Parent=MsG0180005055.01.T01
Chr1 exon 86302500 86303009 86302500 ID=MsG0180005055.01.T01:exon:28391;Parent=MsG0180005055.01.T01
Chr1 CDS 86300538 86301167 86300538 ID=MsG0180005055.01.T01:cds;Parent=MsG0180005055.01.T01
Chr1 CDS 86301249 86301464 86301249 ID=MsG0180005055.01.T01:cds;Parent=MsG0180005055.01.T01
Chr1 CDS 86302500 86303009 86302500 ID=MsG0180005055.01.T01:cds;Parent=MsG0180005055.01.T01
Gene Sequence

>MsG0180005055.01.T01

ATGTACCGGGTAGCAAAAGCATCAGAATATCTTGCGATAACTGGTGTAGGAATCGACGACATAAAACTCGAAAAGAAAGCATGGATTTACCCTGGTCAAAAATGCACGGTCTTCGACCTCTCACCCGTGAACTATACCTTCTTAGTTCAAGCTATGAGTGCCGAAAAACTCCCTTTCGTTCTCCCTGCCGTCTTCACTATCGGTCCTCGTGTTGACGATCATGAAAGCCTACTCAAATACGCCAAACTTATTTCACCCCATGATAGACACTCCAATCATGTTAATGAACTTGTTCAAGGAATCATTGAAGGTGAGACACGTGTTCTAGCTGCTTCCATGACCATGGAGGAGGTTTTCAGAGGAACAAAACAGTTTAAACAAGAAGTATTCGATAAAGTTCAACTTGAGTTGAATCAATTCGGGTTATTGATTTACAATGCGAATGTTAAGCAATTGGTGGATGTGCCTGGTCACGAGTATTTCTCGTATCTGGGACAAAAGACGCAGATGGAAGCAGCGAATCAAGCTAGGGTTGACGTGGCAGAAGCTAAGATGAAAGGTGAAATTGGTTCGAAATTGAGGGAAGGACAAACGCTGCAGAATGCAGCGAAAATTGATGCAGAGACGAAGAGAGAGGCTGAGGTTGCTGAGGCTAATTCTGAGTTGGCCAAGAAGAAAGCGGCTTGGACCAAGGCGGCTCAGGTGGCTGAAGTTGAGGCGAAGAAGGCTGTGGCTCTAAGGGAAGCTGAGTTACAAGGCGAGGTTGAGAAGATGAATGCTCTGACAAAAACTGAGAAGCTTAAAGCTGATTTACTCAGCAAAGCTAGTGTTCAGTATGAAACCAAGGTACAAGAGGCTAACTGGGAATTATACAAGAAACAAAAAGAAGCAGAAGCAATTCTGTATGAGAAGAAGGCAGAGGCAGAAGCGCAAAAAGCATTGGCAGATTCCACGTTTTATGCTCGCAAGCAAGAAGCTGAAGCAGAGCTATACGCAAAGAAAAAAGAGGCAGAGGGGATTGCGACACTTGGGAATGCTCAAGGAGCGTATGTAAGTACACTTCTCAATGCACTTGGAAACAACTACACAGCAGTAAGGGATTACTTGATGATAAATGGTGGCATGTTTCAAGAGATTGCTAAGATTAATGCCGAGGCGGTACGCGGACTTGAACCTAAGATTAGTATTTGGACTAACGGTGGTGATAATGGTGGGGGAATAACTGAAGGTGCTATGGGTATGAAAGAAGTTGCTGGTGTCTACAAGATGTTGCCACCTCTGTTTAAGACTGTCCATGAGCAAACTGGCATGTTGCCTCCTGCTTGGATGGGATCCTTATCTGACAAAAGCTCTTGA
Protein sequence

>MsG0180005055.01.T01

MYRVAKASEYLAITGVGIDDIKLEKKAWIYPGQKCTVFDLSPVNYTFLVQAMSAEKLPFVLPAVFTIGPRVDDHESLLKYAKLISPHDRHSNHVNELVQGIIEGETRVLAASMTMEEVFRGTKQFKQEVFDKVQLELNQFGLLIYNANVKQLVDVPGHEYFSYLGQKTQMEAANQARVDVAEAKMKGEIGSKLREGQTLQNAAKIDAETKREAEVAEANSELAKKKAAWTKAAQVAEVEAKKAVALREAELQGEVEKMNALTKTEKLKADLLSKASVQYETKVQEANWELYKKQKEAEAILYEKKAEAEAQKALADSTFYARKQEAEAELYAKKKEAEGIATLGNAQGAYVSTLLNALGNNYTAVRDYLMINGGMFQEIAKINAEAVRGLEPKISIWTNGGDNGGGITEGAMGMKEVAGVYKMLPPLFKTVHEQTGMLPPAWMGSLSDKSS*