AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004031.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004031.01.T01 MTR_1g073400 97.071 478 10 1 1 478 1 474 0.0 942
MsG0180004031.01.T01 MTR_3g106480 92.259 478 37 0 1 478 1 478 0.0 908
MsG0180004031.01.T01 MTR_1g115145 94.154 479 21 3 1 478 1 473 0.0 888
MsG0180004031.01.T01 MTR_1g071130 91.841 478 10 2 1 478 1 449 0.0 887
MsG0180004031.01.T01 MTR_3g106420 91.632 478 40 0 1 478 3 480 0.0 875
MsG0180004031.01.T01 MTR_3g106485 92.259 478 33 2 1 478 1 474 0.0 874
MsG0180004031.01.T01 MTR_3g106430 91.614 477 36 2 1 477 1 473 0.0 837
MsG0180004031.01.T01 MTR_1g083190 88.912 478 45 1 1 478 3 472 0.0 837
MsG0180004031.01.T01 MTR_3g106470 80.713 477 36 2 1 477 32 452 0.0 737
MsG0180004031.01.T01 MTR_8g063830 80.645 465 49 5 1 464 1 425 0.0 674
MsG0180004031.01.T01 MTR_3g065540 66.087 115 12 1 40 154 14 101 1.49e-41 144
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004031.01.T01 AT5G25260 75.316 474 105 2 1 474 1 462 0.0 739
MsG0180004031.01.T01 AT5G25250 75.264 473 103 2 1 473 1 459 0.0 728
MsG0180004031.01.T01 AT5G64870 71.610 472 123 1 2 473 3 463 0.0 662

Find 123 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGAATATTTCTCATATTT+AGG 0.103686 1:+72173790 MsG0180004031.01.T01:CDS
TGCTTGATACTGTCATATTT+TGG 0.190177 1:+72175620 MsG0180004031.01.T01:three_prime_UTR
TTGAACCTAAGATTAGTATT+TGG 0.251111 1:+72175199 MsG0180004031.01.T01:CDS
CGGAATAACGGAAGGTTCTA+TGG 0.257659 1:+72175245 MsG0180004031.01.T01:CDS
CTGCTAAAGCTGTGGCTCTA+AGG 0.272737 1:+72174118 MsG0180004031.01.T01:CDS
AGGGATTACTTGATGATAAA+TGG 0.295084 1:+72175126 MsG0180004031.01.T01:CDS
TGCTAAATTACCAAAATAAA+AGG 0.295797 1:-72175424 None:intergenic
ATGAATATTTCTCATATTTA+GGG 0.305111 1:+72173791 MsG0180004031.01.T01:CDS
TCCGACAAAAGCTCTTAATT+AGG 0.314591 1:+72175369 MsG0180004031.01.T01:exon
GTGAAGTTGGCTCGAAATCT+AGG 0.314615 1:+72173878 MsG0180004031.01.T01:CDS
TTTAGGTACAAGAGGCTAAC+TGG 0.315931 1:+72174872 MsG0180004031.01.T01:intron
TTAGTCCAAATACTAATCTT+AGG 0.318195 1:-72175204 None:intergenic
CTTCCCGCTGTCTTCACAAT+TGG 0.324959 1:+72173499 MsG0180004031.01.T01:CDS
ATTAGGAGGATGCTTGTATT+AGG 0.327156 1:+72175386 MsG0180004031.01.T01:exon
ACAAGGAGGGATTTAGAGTT+AGG 0.330276 1:+72173980 MsG0180004031.01.T01:CDS
CATGTTGCCTCCTGCTTGGA+TGG 0.342126 1:+72175338 MsG0180004031.01.T01:CDS
TGTAAATCAATAACCCGAAT+TGG 0.349196 1:-72173732 None:intergenic
AAGATTAGTATTTGGACTAA+TGG 0.361897 1:+72175207 MsG0180004031.01.T01:CDS
GAAATGACTACACAGCTCTA+AGG 0.365727 1:+72175106 MsG0180004031.01.T01:CDS
CAATGCGAATGTTAAGCAAT+TGG 0.388576 1:+72173753 MsG0180004031.01.T01:CDS
CAACTCGAACTAAACCAATT+CGG 0.390858 1:+72173718 MsG0180004031.01.T01:CDS
ATCAAAATCAAACGATGTAC+AGG 0.393868 1:+72173305 MsG0180004031.01.T01:exon
TGGTCCTCGTGTTGACGATC+AGG 0.407219 1:+72173519 MsG0180004031.01.T01:CDS
TCAAACTTGCAAAGAAATCA+TGG 0.416941 1:+72173380 MsG0180004031.01.T01:CDS
AACTCGAACTAAACCAATTC+GGG 0.427281 1:+72173719 MsG0180004031.01.T01:CDS
TCCGAGTACCTGGTGATAAC+CGG 0.437519 1:+72173340 MsG0180004031.01.T01:CDS
GATGTCTTCGATTCCAGCTC+CGG 0.437947 1:-72173359 None:intergenic
GAAGAAAGCGGCTTGGACTA+AGG 0.439613 1:+72174071 MsG0180004031.01.T01:CDS
AGCAATTCTGTATGAGAAGA+AGG 0.462456 1:+72174924 MsG0180004031.01.T01:CDS
AAATCAGTTTAGCGTATTTG+AGG 0.472961 1:-72173549 None:intergenic
TTTAGGGCAAAAGACGCAAA+TGG 0.491891 1:+72173807 MsG0180004031.01.T01:CDS
AAGACTGTCCATGAGCAAAC+AGG 0.492575 1:+72175315 MsG0180004031.01.T01:CDS
TGGAAGCAGCGAATCAAGCT+AGG 0.499263 1:+72173827 MsG0180004031.01.T01:CDS
TTGGACTAAGGCGGCTCAAG+TGG 0.501148 1:+72174083 MsG0180004031.01.T01:CDS
TGCTAAAGCTGTGGCTCTAA+GGG 0.501192 1:+72174119 MsG0180004031.01.T01:CDS
CAGGCATGTTGCCTCCTGCT+TGG 0.503335 1:+72175334 MsG0180004031.01.T01:CDS
TGCTTCGATGACCATGGAAG+AGG 0.508530 1:+72173648 MsG0180004031.01.T01:CDS
AGCAAAAGCATCCGAGTACC+TGG 0.509436 1:+72173330 MsG0180004031.01.T01:CDS
TAGAGTTAGGACTGAAGTGA+AGG 0.517399 1:+72173993 MsG0180004031.01.T01:CDS
GCTAAGATGAAAGGTGAAGT+TGG 0.517494 1:+72173865 MsG0180004031.01.T01:CDS
CATGTCAAGGAACTAGTTCA+AGG 0.517510 1:+72173595 MsG0180004031.01.T01:CDS
GCAACATGCCTGTTTGCTCA+TGG 0.521020 1:-72175323 None:intergenic
ATGTTGCCTCCTGCTTGGAT+GGG 0.522936 1:+72175339 MsG0180004031.01.T01:CDS
GTTATTGCTATGCAGAGGGC+TGG 0.525373 1:+72173949 MsG0180004031.01.T01:CDS
TACCTGGTGATAACCGGAGC+TGG 0.525647 1:+72173346 MsG0180004031.01.T01:CDS
ATGGGCATGAAAGAGGTTGC+TGG 0.526365 1:+72175264 MsG0180004031.01.T01:CDS
AAGCAATTGGTGGATGTGCC+TGG 0.526695 1:+72173766 MsG0180004031.01.T01:CDS
TCCTCTGAAAACCTCTTCCA+TGG 0.530446 1:-72173659 None:intergenic
CGAAAGTTATTGCTATGCAG+AGG 0.532272 1:+72173944 MsG0180004031.01.T01:CDS
GGCTCAAGTGGCTGAAGTCG+AGG 0.533715 1:+72174095 MsG0180004031.01.T01:CDS
ATTAGTATTTGGACTAATGG+AGG 0.534214 1:+72175210 MsG0180004031.01.T01:CDS
TTCCAGCTCCGGTTATCACC+AGG 0.538435 1:-72173348 None:intergenic
TGGTGGCATGTTTCAAGAGA+TGG 0.539247 1:+72175146 MsG0180004031.01.T01:CDS
GACAAAAGCTCTTAATTAGG+AGG 0.540934 1:+72175372 MsG0180004031.01.T01:exon
TCCGGTTATCACCAGGTACT+CGG 0.542656 1:-72173341 None:intergenic
GGCTGAGGCTAATTCTGAGT+TGG 0.544080 1:+72174044 MsG0180004031.01.T01:CDS
TGACAATGGAGGCGGAATAA+CGG 0.546090 1:+72175233 MsG0180004031.01.T01:CDS
GCAGAAGGGATTGTGACACT+TGG 0.546780 1:+72175039 MsG0180004031.01.T01:CDS
CACCGTCTTCGACCTCTCAC+CGG 0.548854 1:+72173423 MsG0180004031.01.T01:CDS
GATAAGGTTCCCATCCAAGC+AGG 0.550974 1:-72175348 None:intergenic
AATACACTTCTCAATGCACT+TGG 0.557121 1:+72175084 MsG0180004031.01.T01:CDS
AAGAGAGGCTGAAGTGGCTG+AGG 0.558698 1:+72174029 MsG0180004031.01.T01:CDS
TTGGATGGTTTAGGTACAAG+AGG 0.561865 1:+72174864 MsG0180004031.01.T01:intron
CAAACTTGCAAAGAAATCAT+GGG 0.562132 1:+72173381 MsG0180004031.01.T01:CDS
AGGTTCTATGGGCATGAAAG+AGG 0.562875 1:+72175257 MsG0180004031.01.T01:CDS
TGCGAATGTTAAGCAATTGG+TGG 0.563120 1:+72173756 MsG0180004031.01.T01:CDS
GGACCAATTGTGAAGACAGC+GGG 0.564372 1:-72173502 None:intergenic
CTCAACCTCACCTTGCAACT+CGG 0.566348 1:-72174145 None:intergenic
GGAATAACGGAAGGTTCTAT+GGG 0.567693 1:+72175246 MsG0180004031.01.T01:CDS
CCTCGCTGCTTCGATGACCA+TGG 0.570615 1:+72173642 MsG0180004031.01.T01:CDS
ACTTCGAAGGTATAGTTCAC+CGG 0.571447 1:-72173442 None:intergenic
AAGACGGTGCAAGATTGACC+AGG 0.574032 1:-72173409 None:intergenic
AGGTATAGTTCACCGGTGAG+AGG 0.574045 1:-72173435 None:intergenic
AAATGACTACACAGCTCTAA+GGG 0.574906 1:+72175107 MsG0180004031.01.T01:CDS
AGTTGCAAGGTGAGGTTGAG+AGG 0.575856 1:+72174148 MsG0180004031.01.T01:CDS
TTAGGTACAAGAGGCTAACT+GGG 0.579786 1:+72174873 MsG0180004031.01.T01:intron
TGAAGTTGGCTCGAAATCTA+GGG 0.579862 1:+72173879 MsG0180004031.01.T01:CDS
GTGACACTTGGGAATGCTCA+AGG 0.583871 1:+72175051 MsG0180004031.01.T01:CDS
TGGCGAAGAAGAAAGCGGCT+TGG 0.584288 1:+72174064 MsG0180004031.01.T01:CDS
TCAGTTTAGCGTATTTGAGG+AGG 0.587438 1:-72173546 None:intergenic
AAATATGACAGTATCAAGCA+TGG 0.587802 1:-72175617 None:intergenic
GAATCAAAGAGAGGCTGAAG+TGG 0.589954 1:+72174023 MsG0180004031.01.T01:CDS
CTAAGGGAAGCCGAGTTGCA+AGG 0.593610 1:+72174135 MsG0180004031.01.T01:CDS
CAGAAGGGATTGTGACACTT+GGG 0.598815 1:+72175040 MsG0180004031.01.T01:CDS
TCTGTATGAGAAGAAGGCAG+AGG 0.600808 1:+72174930 MsG0180004031.01.T01:CDS
ACTCATGGCTTGAACTTCGA+AGG 0.601319 1:-72173455 None:intergenic
AATGGAGGTGACAATGGAGG+CGG 0.607583 1:+72175225 MsG0180004031.01.T01:CDS
TGGACTAATGGAGGTGACAA+TGG 0.609237 1:+72175219 MsG0180004031.01.T01:CDS
GCTGGAGAGAGTGACAAGGA+GGG 0.609527 1:+72173967 MsG0180004031.01.T01:CDS
CTAGTTCAAGGAATCATCGA+AGG 0.612313 1:+72173607 MsG0180004031.01.T01:CDS
GTGGCAGAAGCTAAGATGAA+AGG 0.614706 1:+72173856 MsG0180004031.01.T01:CDS
AATATGACAGTATCAAGCAT+GGG 0.616101 1:-72175616 None:intergenic
GGCTGGAGAGAGTGACAAGG+AGG 0.627665 1:+72173966 MsG0180004031.01.T01:CDS
CACCGGTGAGAGGTCGAAGA+CGG 0.628279 1:-72173425 None:intergenic
AAGACAGCGGGAAGAACGAA+AGG 0.630029 1:-72173490 None:intergenic
GGAAGCAGCGAATCAAGCTA+GGG 0.630802 1:+72173828 MsG0180004031.01.T01:CDS
TAGTTCAAGGAATCATCGAA+GGG 0.630851 1:+72173608 MsG0180004031.01.T01:CDS
GAAAGTTATTGCTATGCAGA+GGG 0.630907 1:+72173945 MsG0180004031.01.T01:CDS
ATCAATGCTGAGGCAGTACG+TGG 0.635980 1:+72175174 MsG0180004031.01.T01:CDS
TATGAGAAATATTCATGACC+AGG 0.636387 1:-72173784 None:intergenic
TCAAAATCAAACGATGTACA+GGG 0.641066 1:+72173306 MsG0180004031.01.T01:exon
TAGTGTTGAGTATGAAACCA+AGG 0.644971 1:+72174224 MsG0180004031.01.T01:CDS
GAGGGCTGGAGAGAGTGACA+AGG 0.646522 1:+72173963 MsG0180004031.01.T01:CDS
GGAAGCCGAGTTGCAAGGTG+AGG 0.648282 1:+72174140 MsG0180004031.01.T01:CDS
TGAGTTGGCGAAGAAGAAAG+CGG 0.653130 1:+72174059 MsG0180004031.01.T01:CDS
TAAACTCTCGAATCATGTCA+AGG 0.655320 1:+72173582 MsG0180004031.01.T01:CDS
TGGACAGTCTTAAACAGAGG+TGG 0.656626 1:-72175303 None:intergenic
GATTACTTGATGATAAATGG+TGG 0.658453 1:+72175129 MsG0180004031.01.T01:CDS
TCATGGACAGTCTTAAACAG+AGG 0.660499 1:-72175306 None:intergenic
AGTCGAGGCTGCTAAAGCTG+TGG 0.666475 1:+72174110 MsG0180004031.01.T01:CDS
AATGGAGGCGGAATAACGGA+AGG 0.668536 1:+72175237 MsG0180004031.01.T01:CDS
AAGGTTCCCATCCAAGCAGG+AGG 0.669932 1:-72175345 None:intergenic
GTTGGCTCGAAATCTAGGGA+AGG 0.671253 1:+72173883 MsG0180004031.01.T01:CDS
ACTAATGGAGGTGACAATGG+AGG 0.677352 1:+72175222 MsG0180004031.01.T01:CDS
TGATTCGAGAGTTTATCATG+AGG 0.680428 1:-72173574 None:intergenic
GATGGCTAAGATCAATGCTG+AGG 0.682349 1:+72175164 MsG0180004031.01.T01:CDS
GAATCAAGCTAGGGTTGACG+TGG 0.682564 1:+72173837 MsG0180004031.01.T01:CDS
CTTTCCTGATCGTCAACACG+AGG 0.689163 1:-72173523 None:intergenic
AGACGGTGCAAGATTGACCA+GGG 0.716272 1:-72173408 None:intergenic
AGTTCAAGGAATCATCGAAG+GGG 0.721843 1:+72173609 MsG0180004031.01.T01:CDS
AGGACCAATTGTGAAGACAG+CGG 0.726189 1:-72173503 None:intergenic
CCATGGTCATCGAAGCAGCG+AGG 0.728643 1:-72173642 None:intergenic
GAAAGCGGCTTGGACTAAGG+CGG 0.747622 1:+72174074 MsG0180004031.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATTAATAAATGAAAGA+AGG - Chr1:72174271-72174290 None:intergenic 10.0%
!! ATGAATATTTCTCATATTTA+GGG + Chr1:72173791-72173810 MsG0180004031.01.T01:CDS 15.0%
!! CATTATAAAACTAAATCATA+TGG + Chr1:72174498-72174517 MsG0180004031.01.T01:intron 15.0%
!! TAATGTTAAAAAAATTGACA+AGG - Chr1:72174754-72174773 None:intergenic 15.0%
!!! AATGTTTATTTTTTAAGTAC+TGG + Chr1:72174833-72174852 MsG0180004031.01.T01:intron 15.0%
!!! AATTTACAATTTTTCATGAT+TGG + Chr1:72175689-72175708 MsG0180004031.01.T01:three_prime_UTR 15.0%
!!! TGTTCTAAATTAATTTTGTT+AGG + Chr1:72174554-72174573 MsG0180004031.01.T01:intron 15.0%
!! ATGAAAAATTAGCATACTTA+TGG - Chr1:72175546-72175565 None:intergenic 20.0%
!! CATGAATATTTCTCATATTT+AGG + Chr1:72173790-72173809 MsG0180004031.01.T01:CDS 20.0%
!! TGCTAAATTACCAAAATAAA+AGG - Chr1:72175427-72175446 None:intergenic 20.0%
!! TTAAAAAAATTGACAAGGTT+TGG - Chr1:72174749-72174768 None:intergenic 20.0%
!!! TATGGAATAGTCAAATTTTT+TGG + Chr1:72174440-72174459 MsG0180004031.01.T01:intron 20.0%
!!! TTAATATGTTATATGGACTA+TGG + Chr1:72174422-72174441 MsG0180004031.01.T01:intron 20.0%
! AAAACAAAACGATTGACATA+TGG + Chr1:72174676-72174695 MsG0180004031.01.T01:intron 25.0%
! AAGATTAGTATTTGGACTAA+TGG + Chr1:72175207-72175226 MsG0180004031.01.T01:CDS 25.0%
! ACAAAATAATAGCAAGCAAA+AGG - Chr1:72174605-72174624 None:intergenic 25.0%
! ATTGACATATGGTATGTAAA+TGG + Chr1:72174687-72174706 MsG0180004031.01.T01:intron 25.0%
! GAAGTAAAACATAACAAAGT+AGG + Chr1:72174786-72174805 MsG0180004031.01.T01:intron 25.0%
! TTAGTCCAAATACTAATCTT+AGG - Chr1:72175207-72175226 None:intergenic 25.0%
! TTGAACCTAAGATTAGTATT+TGG + Chr1:72175199-72175218 MsG0180004031.01.T01:CDS 25.0%
!! AGTAATGTATTTTTCATGCT+TGG + Chr1:72175578-72175597 MsG0180004031.01.T01:three_prime_UTR 25.0%
!! CATGATTGGTATTTTATGAA+TGG + Chr1:72175703-72175722 MsG0180004031.01.T01:three_prime_UTR 25.0%
!! TATGCATGGTATTTTATGAA+TGG + Chr1:72175498-72175517 MsG0180004031.01.T01:three_prime_UTR 25.0%
!!! AGTAGCAATACCTTTTATTT+TGG + Chr1:72175414-72175433 MsG0180004031.01.T01:three_prime_UTR 25.0%
!!! GCTATTATTTTGTGTACTTT+TGG + Chr1:72174612-72174631 MsG0180004031.01.T01:intron 25.0%
!!! TCACTGTAGTTAAAGTTTTT+TGG + Chr1:72174357-72174376 MsG0180004031.01.T01:intron 25.0%
!!! TTAAGTACTGGTTGAAATTT+TGG + Chr1:72174845-72174864 MsG0180004031.01.T01:intron 25.0%
AAATATGACAGTATCAAGCA+TGG - Chr1:72175620-72175639 None:intergenic 30.0%
AAATCAGTTTAGCGTATTTG+AGG - Chr1:72173552-72173571 None:intergenic 30.0%
AAGAAGGAGTTATATTTGCA+TGG - Chr1:72174255-72174274 None:intergenic 30.0%
AATATGACAGTATCAAGCAT+GGG - Chr1:72175619-72175638 None:intergenic 30.0%
ATCAAAATCAAACGATGTAC+AGG + Chr1:72173305-72173324 MsG0180004031.01.T01:exon 30.0%
ATTAGTATTTGGACTAATGG+AGG + Chr1:72175210-72175229 MsG0180004031.01.T01:CDS 30.0%
CAAACTTGCAAAGAAATCAT+GGG + Chr1:72173381-72173400 MsG0180004031.01.T01:CDS 30.0%
GGCAAATAGATTGAAGAATA+TGG + Chr1:72174708-72174727 MsG0180004031.01.T01:intron 30.0%
TATGAGAAATATTCATGACC+AGG - Chr1:72173787-72173806 None:intergenic 30.0%
TCAAAATCAAACGATGTACA+GGG + Chr1:72173306-72173325 MsG0180004031.01.T01:exon 30.0%
TCAAACTTGCAAAGAAATCA+TGG + Chr1:72173380-72173399 MsG0180004031.01.T01:CDS 30.0%
TGTAAATCAATAACCCGAAT+TGG - Chr1:72173735-72173754 None:intergenic 30.0%
! AAAAATTGACAAGGTTTGGT+TGG - Chr1:72174745-72174764 None:intergenic 30.0%
! AGGGATTACTTGATGATAAA+TGG + Chr1:72175126-72175145 MsG0180004031.01.T01:CDS 30.0%
! GATTACTTGATGATAAATGG+TGG + Chr1:72175129-72175148 MsG0180004031.01.T01:CDS 30.0%
! GTATGCTAATTTTTCATGCA+TGG + Chr1:72175549-72175568 MsG0180004031.01.T01:three_prime_UTR 30.0%
! GTTGAAATTTTGGATGGTTT+AGG + Chr1:72174855-72174874 MsG0180004031.01.T01:intron 30.0%
! GTTTCAATTTTCTTGTCAAG+AGG + Chr1:72173268-72173287 MsG0180004031.01.T01:five_prime_UTR 30.0%
! TCCTAATTAAGAGCTTTTGT+CGG - Chr1:72175373-72175392 None:intergenic 30.0%
! TGCTTGATACTGTCATATTT+TGG + Chr1:72175620-72175639 MsG0180004031.01.T01:three_prime_UTR 30.0%
! TTTTCATGCTTGGAAATGAA+TGG + Chr1:72175588-72175607 MsG0180004031.01.T01:three_prime_UTR 30.0%
!!! ACTAGTCTTTTTCATATGCA+TGG + Chr1:72175484-72175503 MsG0180004031.01.T01:three_prime_UTR 30.0%
AACTCGAACTAAACCAATTC+GGG + Chr1:72173719-72173738 MsG0180004031.01.T01:CDS 35.0%
AATACACTTCTCAATGCACT+TGG + Chr1:72175084-72175103 MsG0180004031.01.T01:CDS 35.0%
AGCAATTCTGTATGAGAAGA+AGG + Chr1:72174924-72174943 MsG0180004031.01.T01:CDS 35.0%
ATTAGGAGGATGCTTGTATT+AGG + Chr1:72175386-72175405 MsG0180004031.01.T01:exon 35.0%
CAAAGAAAAAAGAGGCAGAA+GGG + Chr1:72175025-72175044 MsG0180004031.01.T01:CDS 35.0%
CAACTCGAACTAAACCAATT+CGG + Chr1:72173718-72173737 MsG0180004031.01.T01:CDS 35.0%
CAATGCGAATGTTAAGCAAT+TGG + Chr1:72173753-72173772 MsG0180004031.01.T01:CDS 35.0%
GAAAGTTATTGCTATGCAGA+GGG + Chr1:72173945-72173964 MsG0180004031.01.T01:CDS 35.0%
GACAAAAGCTCTTAATTAGG+AGG + Chr1:72175372-72175391 MsG0180004031.01.T01:exon 35.0%
GCTATACGCAAAGAAAAAAG+AGG + Chr1:72175017-72175036 MsG0180004031.01.T01:CDS 35.0%
TAAACTCTCGAATCATGTCA+AGG + Chr1:72173582-72173601 MsG0180004031.01.T01:CDS 35.0%
TAGTTCAAGGAATCATCGAA+GGG + Chr1:72173608-72173627 MsG0180004031.01.T01:CDS 35.0%
TCCGACAAAAGCTCTTAATT+AGG + Chr1:72175369-72175388 MsG0180004031.01.T01:exon 35.0%
TGATTCGAGAGTTTATCATG+AGG - Chr1:72173577-72173596 None:intergenic 35.0%
! AAATGACTACACAGCTCTAA+GGG + Chr1:72175107-72175126 MsG0180004031.01.T01:CDS 35.0%
! AAGAACGAAAGGCAGTTTTT+CGG - Chr1:72173482-72173501 None:intergenic 35.0%
! ATATTTGCATGGACTAACCT+TGG - Chr1:72174244-72174263 None:intergenic 35.0%
! TAGTGTTGAGTATGAAACCA+AGG + Chr1:72174224-72174243 MsG0180004031.01.T01:CDS 35.0%
! TTAAGAGCTTTTGTCGGATA+AGG - Chr1:72175367-72175386 None:intergenic 35.0%
!! GGTTTTTGAGAATCAAAGAG+AGG + Chr1:72174014-72174033 MsG0180004031.01.T01:CDS 35.0%
!!! GTACTGGTTGAAATTTTGGA+TGG + Chr1:72174849-72174868 MsG0180004031.01.T01:intron 35.0%
AAGGATCCACGATTTAGACT+TGG - Chr1:72174586-72174605 None:intergenic 40.0%
AATGTGCCAAGTCTAAATCG+TGG + Chr1:72174577-72174596 MsG0180004031.01.T01:intron 40.0%
AGCAAGTGCTATGTTTCGTT+TGG + Chr1:72174651-72174670 MsG0180004031.01.T01:intron 40.0%
AGTTCAAGGAATCATCGAAG+GGG + Chr1:72173609-72173628 MsG0180004031.01.T01:CDS 40.0%
CATGTCAAGGAACTAGTTCA+AGG + Chr1:72173595-72173614 MsG0180004031.01.T01:CDS 40.0%
CGAAAGTTATTGCTATGCAG+AGG + Chr1:72173944-72173963 MsG0180004031.01.T01:CDS 40.0%
CTAGTTCAAGGAATCATCGA+AGG + Chr1:72173607-72173626 MsG0180004031.01.T01:CDS 40.0%
GAAATGACTACACAGCTCTA+AGG + Chr1:72175106-72175125 MsG0180004031.01.T01:CDS 40.0%
GAATGCAGCAAAAATTGACG+CGG + Chr1:72173918-72173937 MsG0180004031.01.T01:CDS 40.0%
GCAAAGAAAAAAGAGGCAGA+AGG + Chr1:72175024-72175043 MsG0180004031.01.T01:CDS 40.0%
GCTAAGATGAAAGGTGAAGT+TGG + Chr1:72173865-72173884 MsG0180004031.01.T01:CDS 40.0%
TAGAGTTAGGACTGAAGTGA+AGG + Chr1:72173993-72174012 MsG0180004031.01.T01:CDS 40.0%
TCAGTTTAGCGTATTTGAGG+AGG - Chr1:72173549-72173568 None:intergenic 40.0%
TCATGGACAGTCTTAAACAG+AGG - Chr1:72175309-72175328 None:intergenic 40.0%
TGCGAATGTTAAGCAATTGG+TGG + Chr1:72173756-72173775 MsG0180004031.01.T01:CDS 40.0%
TTAGGTACAAGAGGCTAACT+GGG + Chr1:72174873-72174892 MsG0180004031.01.T01:intron 40.0%
TTGGATGGTTTAGGTACAAG+AGG + Chr1:72174864-72174883 MsG0180004031.01.T01:intron 40.0%
TTTAGGTACAAGAGGCTAAC+TGG + Chr1:72174872-72174891 MsG0180004031.01.T01:intron 40.0%
! AAGAAATCATGGGTTTTCCC+TGG + Chr1:72173391-72173410 MsG0180004031.01.T01:CDS 40.0%
! ACAAGGAGGGATTTAGAGTT+AGG + Chr1:72173980-72173999 MsG0180004031.01.T01:CDS 40.0%
! ACTTCGAAGGTATAGTTCAC+CGG - Chr1:72173445-72173464 None:intergenic 40.0%
! GGAATAACGGAAGGTTCTAT+GGG + Chr1:72175246-72175265 MsG0180004031.01.T01:CDS 40.0%
! TTTAGGGCAAAAGACGCAAA+TGG + Chr1:72173807-72173826 MsG0180004031.01.T01:CDS 40.0%
!! TGAAGTTGGCTCGAAATCTA+GGG + Chr1:72173879-72173898 MsG0180004031.01.T01:CDS 40.0%
AAGACTGTCCATGAGCAAAC+AGG + Chr1:72175315-72175334 MsG0180004031.01.T01:CDS 45.0%
ACTAATGGAGGTGACAATGG+AGG + Chr1:72175222-72175241 MsG0180004031.01.T01:CDS 45.0%
ACTCATGGCTTGAACTTCGA+AGG - Chr1:72173458-72173477 None:intergenic 45.0%
AGGACCAATTGTGAAGACAG+CGG - Chr1:72173506-72173525 None:intergenic 45.0%
AGGTTCTATGGGCATGAAAG+AGG + Chr1:72175257-72175276 MsG0180004031.01.T01:CDS 45.0%
CAGAAGGGATTGTGACACTT+GGG + Chr1:72175040-72175059 MsG0180004031.01.T01:CDS 45.0%
GAATCAAAGAGAGGCTGAAG+TGG + Chr1:72174023-72174042 MsG0180004031.01.T01:CDS 45.0%
GATGGCTAAGATCAATGCTG+AGG + Chr1:72175164-72175183 MsG0180004031.01.T01:CDS 45.0%
GTGGCAGAAGCTAAGATGAA+AGG + Chr1:72173856-72173875 MsG0180004031.01.T01:CDS 45.0%
TCTGTATGAGAAGAAGGCAG+AGG + Chr1:72174930-72174949 MsG0180004031.01.T01:CDS 45.0%
TGACAATGGAGGCGGAATAA+CGG + Chr1:72175233-72175252 MsG0180004031.01.T01:CDS 45.0%
TGGACAGTCTTAAACAGAGG+TGG - Chr1:72175306-72175325 None:intergenic 45.0%
TGGACTAATGGAGGTGACAA+TGG + Chr1:72175219-72175238 MsG0180004031.01.T01:CDS 45.0%
TGGTGGCATGTTTCAAGAGA+TGG + Chr1:72175146-72175165 MsG0180004031.01.T01:CDS 45.0%
! ACCATGGAAGAGGTTTTCAG+AGG + Chr1:72173658-72173677 MsG0180004031.01.T01:CDS 45.0%
! CGGAATAACGGAAGGTTCTA+TGG + Chr1:72175245-72175264 MsG0180004031.01.T01:CDS 45.0%
! TCCTCTGAAAACCTCTTCCA+TGG - Chr1:72173662-72173681 None:intergenic 45.0%
! TGAGTTGGCGAAGAAGAAAG+CGG + Chr1:72174059-72174078 MsG0180004031.01.T01:CDS 45.0%
!! GTGAAGTTGGCTCGAAATCT+AGG + Chr1:72173878-72173897 MsG0180004031.01.T01:CDS 45.0%
!! TGCTAAAGCTGTGGCTCTAA+GGG + Chr1:72174119-72174138 MsG0180004031.01.T01:CDS 45.0%
!!! TTATTTTTTAATATGTTATA+TGG + Chr1:72174415-72174434 MsG0180004031.01.T01:intron 5.0%
AAGACAGCGGGAAGAACGAA+AGG - Chr1:72173493-72173512 None:intergenic 50.0%
AAGACGGTGCAAGATTGACC+AGG - Chr1:72173412-72173431 None:intergenic 50.0%
AATGGAGGTGACAATGGAGG+CGG + Chr1:72175225-72175244 MsG0180004031.01.T01:CDS 50.0%
AGACGGTGCAAGATTGACCA+GGG - Chr1:72173411-72173430 None:intergenic 50.0%
AGGTATAGTTCACCGGTGAG+AGG - Chr1:72173438-72173457 None:intergenic 50.0%
AGTTGCAAGGTGAGGTTGAG+AGG + Chr1:72174148-72174167 MsG0180004031.01.T01:CDS 50.0%
ATCAATGCTGAGGCAGTACG+TGG + Chr1:72175174-72175193 MsG0180004031.01.T01:CDS 50.0%
ATGGGCATGAAAGAGGTTGC+TGG + Chr1:72175264-72175283 MsG0180004031.01.T01:CDS 50.0%
ATGTTGCCTCCTGCTTGGAT+GGG + Chr1:72175339-72175358 MsG0180004031.01.T01:CDS 50.0%
CTCAACCTCACCTTGCAACT+CGG - Chr1:72174148-72174167 None:intergenic 50.0%
CTTCCCGCTGTCTTCACAAT+TGG + Chr1:72173499-72173518 MsG0180004031.01.T01:CDS 50.0%
CTTTCCTGATCGTCAACACG+AGG - Chr1:72173526-72173545 None:intergenic 50.0%
GAATCAAGCTAGGGTTGACG+TGG + Chr1:72173837-72173856 MsG0180004031.01.T01:CDS 50.0%
GATAAGGTTCCCATCCAAGC+AGG - Chr1:72175351-72175370 None:intergenic 50.0%
GATGTCTTCGATTCCAGCTC+CGG - Chr1:72173362-72173381 None:intergenic 50.0%
GCAACATGCCTGTTTGCTCA+TGG - Chr1:72175326-72175345 None:intergenic 50.0%
GCAGAAGGGATTGTGACACT+TGG + Chr1:72175039-72175058 MsG0180004031.01.T01:CDS 50.0%
GGAAGCAGCGAATCAAGCTA+GGG + Chr1:72173828-72173847 MsG0180004031.01.T01:CDS 50.0%
GGACCAATTGTGAAGACAGC+GGG - Chr1:72173505-72173524 None:intergenic 50.0%
GGCTGAGGCTAATTCTGAGT+TGG + Chr1:72174044-72174063 MsG0180004031.01.T01:CDS 50.0%
GTGACACTTGGGAATGCTCA+AGG + Chr1:72175051-72175070 MsG0180004031.01.T01:CDS 50.0%
GTTATTGCTATGCAGAGGGC+TGG + Chr1:72173949-72173968 MsG0180004031.01.T01:CDS 50.0%
TCCGAGTACCTGGTGATAAC+CGG + Chr1:72173340-72173359 MsG0180004031.01.T01:CDS 50.0%
TCCGGTTATCACCAGGTACT+CGG - Chr1:72173344-72173363 None:intergenic 50.0%
TGCTTCGATGACCATGGAAG+AGG + Chr1:72173648-72173667 MsG0180004031.01.T01:CDS 50.0%
TGGAAGCAGCGAATCAAGCT+AGG + Chr1:72173827-72173846 MsG0180004031.01.T01:CDS 50.0%
! AAGCAATTGGTGGATGTGCC+TGG + Chr1:72173766-72173785 MsG0180004031.01.T01:CDS 50.0%
! AATGGAGGCGGAATAACGGA+AGG + Chr1:72175237-72175256 MsG0180004031.01.T01:CDS 50.0%
! CTGCTAAAGCTGTGGCTCTA+AGG + Chr1:72174118-72174137 MsG0180004031.01.T01:CDS 50.0%
! GAAGAAAGCGGCTTGGACTA+AGG + Chr1:72174071-72174090 MsG0180004031.01.T01:CDS 50.0%
!! AGCAAAAGCATCCGAGTACC+TGG + Chr1:72173330-72173349 MsG0180004031.01.T01:CDS 50.0%
!! AGGCAGTTTTTCGGCACTCA+TGG - Chr1:72173473-72173492 None:intergenic 50.0%
!! GTTGGCTCGAAATCTAGGGA+AGG + Chr1:72173883-72173902 MsG0180004031.01.T01:CDS 50.0%
AAGAGAGGCTGAAGTGGCTG+AGG + Chr1:72174029-72174048 MsG0180004031.01.T01:CDS 55.0%
AAGGTTCCCATCCAAGCAGG+AGG - Chr1:72175348-72175367 None:intergenic 55.0%
AGTCGAGGCTGCTAAAGCTG+TGG + Chr1:72174110-72174129 MsG0180004031.01.T01:CDS 55.0%
CATGTTGCCTCCTGCTTGGA+TGG + Chr1:72175338-72175357 MsG0180004031.01.T01:CDS 55.0%
CTAAGGGAAGCCGAGTTGCA+AGG + Chr1:72174135-72174154 MsG0180004031.01.T01:CDS 55.0%
GCTGGAGAGAGTGACAAGGA+GGG + Chr1:72173967-72173986 MsG0180004031.01.T01:CDS 55.0%
TACCTGGTGATAACCGGAGC+TGG + Chr1:72173346-72173365 MsG0180004031.01.T01:CDS 55.0%
TGGCGAAGAAGAAAGCGGCT+TGG + Chr1:72174064-72174083 MsG0180004031.01.T01:CDS 55.0%
TTCCAGCTCCGGTTATCACC+AGG - Chr1:72173351-72173370 None:intergenic 55.0%
TTGGACTAAGGCGGCTCAAG+TGG + Chr1:72174083-72174102 MsG0180004031.01.T01:CDS 55.0%
! GAAAGCGGCTTGGACTAAGG+CGG + Chr1:72174074-72174093 MsG0180004031.01.T01:CDS 55.0%
! TGGTCCTCGTGTTGACGATC+AGG + Chr1:72173519-72173538 MsG0180004031.01.T01:CDS 55.0%
CACCGGTGAGAGGTCGAAGA+CGG - Chr1:72173428-72173447 None:intergenic 60.0%
CACCGTCTTCGACCTCTCAC+CGG + Chr1:72173423-72173442 MsG0180004031.01.T01:CDS 60.0%
CAGGCATGTTGCCTCCTGCT+TGG + Chr1:72175334-72175353 MsG0180004031.01.T01:CDS 60.0%
CCATGGTCATCGAAGCAGCG+AGG - Chr1:72173645-72173664 None:intergenic 60.0%
CCTCGCTGCTTCGATGACCA+TGG + Chr1:72173642-72173661 MsG0180004031.01.T01:CDS 60.0%
GAGGGCTGGAGAGAGTGACA+AGG + Chr1:72173963-72173982 MsG0180004031.01.T01:CDS 60.0%
GGAAGCCGAGTTGCAAGGTG+AGG + Chr1:72174140-72174159 MsG0180004031.01.T01:CDS 60.0%
GGCTCAAGTGGCTGAAGTCG+AGG + Chr1:72174095-72174114 MsG0180004031.01.T01:CDS 60.0%
GGCTGGAGAGAGTGACAAGG+AGG + Chr1:72173966-72173985 MsG0180004031.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 72173255 72175733 72173255 ID=MsG0180004031.01;Name=MsG0180004031.01
Chr1 mRNA 72173255 72175733 72173255 ID=MsG0180004031.01.T01;Parent=MsG0180004031.01;Name=MsG0180004031.01.T01;_AED=0.29;_eAED=0.29;_QI=64|1|1|1|1|1|2|347|478
Chr1 exon 72173255 72174245 72173255 ID=MsG0180004031.01.T01:exon:31040;Parent=MsG0180004031.01.T01
Chr1 exon 72174877 72175733 72174877 ID=MsG0180004031.01.T01:exon:31041;Parent=MsG0180004031.01.T01
Chr1 five_prime_UTR 72173255 72173318 72173255 ID=MsG0180004031.01.T01:five_prime_utr;Parent=MsG0180004031.01.T01
Chr1 CDS 72173319 72174245 72173319 ID=MsG0180004031.01.T01:cds;Parent=MsG0180004031.01.T01
Chr1 CDS 72174877 72175386 72174877 ID=MsG0180004031.01.T01:cds;Parent=MsG0180004031.01.T01
Chr1 three_prime_UTR 72175387 72175733 72175387 ID=MsG0180004031.01.T01:three_prime_utr;Parent=MsG0180004031.01.T01
Gene Sequence

>MsG0180004031.01.T01

ATGTACAGGGTAGCAAAAGCATCCGAGTACCTGGTGATAACCGGAGCTGGAATCGAAGACATCAAACTTGCAAAGAAATCATGGGTTTTCCCTGGTCAATCTTGCACCGTCTTCGACCTCTCACCGGTGAACTATACCTTCGAAGTTCAAGCCATGAGTGCCGAAAAACTGCCTTTCGTTCTTCCCGCTGTCTTCACAATTGGTCCTCGTGTTGACGATCAGGAAAGCCTCCTCAAATACGCTAAACTGATTTCACCTCATGATAAACTCTCGAATCATGTCAAGGAACTAGTTCAAGGAATCATCGAAGGGGAGACACGTGTCCTCGCTGCTTCGATGACCATGGAAGAGGTTTTCAGAGGAACAAAAGAGTTTAAACAAGAAGTATTCGAGAAAGTTCAACTCGAACTAAACCAATTCGGGTTATTGATTTACAATGCGAATGTTAAGCAATTGGTGGATGTGCCTGGTCATGAATATTTCTCATATTTAGGGCAAAAGACGCAAATGGAAGCAGCGAATCAAGCTAGGGTTGACGTGGCAGAAGCTAAGATGAAAGGTGAAGTTGGCTCGAAATCTAGGGAAGGACAAACACTGCAGAATGCAGCAAAAATTGACGCGGAGACGAAAGTTATTGCTATGCAGAGGGCTGGAGAGAGTGACAAGGAGGGATTTAGAGTTAGGACTGAAGTGAAGGTTTTTGAGAATCAAAGAGAGGCTGAAGTGGCTGAGGCTAATTCTGAGTTGGCGAAGAAGAAAGCGGCTTGGACTAAGGCGGCTCAAGTGGCTGAAGTCGAGGCTGCTAAAGCTGTGGCTCTAAGGGAAGCCGAGTTGCAAGGTGAGGTTGAGAGGATGAATGCTTTGACTACTACTGAGAAGCTTAAAGCTGATTTACTTAGCAAAGCTAGTGTTGAGTATGAAACCAAGGTACAAGAGGCTAACTGGGAATTATACAAGAAACAAAAAGAAGCAGAAGCAATTCTGTATGAGAAGAAGGCAGAGGCAGAAGCACAGAAAGCATTGTCAGATGCAACATTTTATGCGCGCAAGCAAGCAGCTGAAGCAGAGCTATACGCAAAGAAAAAAGAGGCAGAAGGGATTGTGACACTTGGGAATGCTCAAGGAGTGTATGTAAATACACTTCTCAATGCACTTGGAAATGACTACACAGCTCTAAGGGATTACTTGATGATAAATGGTGGCATGTTTCAAGAGATGGCTAAGATCAATGCTGAGGCAGTACGTGGACTTGAACCTAAGATTAGTATTTGGACTAATGGAGGTGACAATGGAGGCGGAATAACGGAAGGTTCTATGGGCATGAAAGAGGTTGCTGGTGTTTACAAGATGCTGCCACCTCTGTTTAAGACTGTCCATGAGCAAACAGGCATGTTGCCTCCTGCTTGGATGGGAACCTTATCCGACAAAAGCTCTTAA
Protein sequence

>MsG0180004031.01.T01

MYRVAKASEYLVITGAGIEDIKLAKKSWVFPGQSCTVFDLSPVNYTFEVQAMSAEKLPFVLPAVFTIGPRVDDQESLLKYAKLISPHDKLSNHVKELVQGIIEGETRVLAASMTMEEVFRGTKEFKQEVFEKVQLELNQFGLLIYNANVKQLVDVPGHEYFSYLGQKTQMEAANQARVDVAEAKMKGEVGSKSREGQTLQNAAKIDAETKVIAMQRAGESDKEGFRVRTEVKVFENQREAEVAEANSELAKKKAAWTKAAQVAEVEAAKAVALREAELQGEVERMNALTTTEKLKADLLSKASVEYETKVQEANWELYKKQKEAEAILYEKKAEAEAQKALSDATFYARKQAAEAELYAKKKEAEGIVTLGNAQGVYVNTLLNALGNDYTALRDYLMINGGMFQEMAKINAEAVRGLEPKISIWTNGGDNGGGITEGSMGMKEVAGVYKMLPPLFKTVHEQTGMLPPAWMGTLSDKSS*