AlfalfaGEDB | MsG0180004815.01

M. sativa cultivar ZhongmuNo.1 / MsG0180004815.01

KEGG: K09584
GO: GO:0005783 GO:0016853 GO:0045454
PFAM: PF00085 PF07749

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004815.01.T01	XP_003591444.2	98.626	364	5	0	77	440	1	364	0	729

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004815.01.T01	P38661	98.352	364	6	0	77	440	1	364	0	728

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004815.01.T01	G7IDU4	98.626	364	5	0	77	440	1	364	0.0	729

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0180001256.01	MsG0180004815.01	PPI
MsG0180001256.01	MsG0180004815.01	PPI
MsG0180004757.01	MsG0180004815.01	PPI
MsG0180004815.01	MsG0380015370.01	PPI
MsG0180004815.01	MsG0380015370.01	PPI
MsG0180004815.01	MsG0880043422.01	PPI
MsG0180004815.01	MsG0680031000.01	PPI
MsG0180004815.01	MsG0780041282.01	PPI
MsG0180004815.01	MsG0480023065.01	PPI
MsG0180004815.01	MsG0380016898.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004815.01.T01	MTR_1g087520	98.626	364	5	0	77	440	1	364	0.0	729
MsG0180004815.01.T01	MTR_7g112040	69.663	356	108	0	84	439	3	358	0.0	527
MsG0180004815.01.T01	MTR_5g084930	34.701	268	151	6	86	334	5	267	5.04e-41	151
MsG0180004815.01.T01	MTR_5g084930	40.741	135	72	4	103	235	156	284	8.51e-20	91.7
MsG0180004815.01.T01	MTR_2g021750	50.000	104	49	2	223	326	28	128	2.34e-23	100
MsG0180004815.01.T01	MTR_2g021750	38.583	127	70	4	86	207	5	128	6.72e-17	82.0
MsG0180004815.01.T01	MTR_2g094180	35.616	219	119	8	107	310	290	501	4.79e-23	102
MsG0180004815.01.T01	MTR_2g094180	37.209	172	88	4	106	276	79	231	2.55e-18	87.8
MsG0180004815.01.T01	MTR_2g094180	38.857	175	90	7	225	391	80	245	2.89e-17	84.3
MsG0180004815.01.T01	MTR_2g094180	34.483	116	71	3	106	218	417	530	1.02e-13	73.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004815.01.T01	AT2G47470	78.788	330	70	0	110	439	28	357	0.0	546
MsG0180004815.01.T01	AT2G47470	73.939	330	60	1	110	439	28	331	1.09e-176	497
MsG0180004815.01.T01	AT2G47470	80.576	278	54	0	110	387	28	305	9.26e-167	472
MsG0180004815.01.T01	AT2G47470	81.590	239	44	0	110	348	28	266	1.62e-145	416
MsG0180004815.01.T01	AT1G04980	34.364	291	151	7	103	373	30	300	1.37e-41	153
MsG0180004815.01.T01	AT2G32920	33.681	288	154	7	103	373	28	295	4.71e-41	152
MsG0180004815.01.T01	AT5G60640	34.884	172	92	4	106	276	104	256	2.51e-22	99.8
MsG0180004815.01.T01	AT5G60640	46.591	88	45	1	225	312	379	464	3.01e-19	90.5
MsG0180004815.01.T01	AT5G60640	34.028	144	86	4	105	243	377	516	4.02e-14	74.7
MsG0180004815.01.T01	AT5G60640	34.884	172	92	4	106	276	104	256	2.81e-22	99.8

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Find 116 sgRNAs with CRISPR-Local

Find 373 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGACAGCTTACTTCTG+AGG	0.602957	1:+83149499	MsG0180004815.01.T01:CDS
AAAATTCCTGTAGGTTTGAA+GGG	0.547840	1:+83148107	MsG0180004815.01.T01:intron
AAACTCAACCAAGACATCTT+TGG	0.333568	1:-83148623	None:intergenic
AAAGCCGGTGAAGATTATGG+AGG	0.748922	1:+83149413	MsG0180004815.01.T01:CDS
AAATCCCAAATCTCTATGTG+GGG	0.651185	1:+83144452	MsG0180004815.01.T01:CDS
AAATGGAGATGCATCAGATC+TGG	0.477555	1:+83146114	MsG0180004815.01.T01:CDS
AACAAAGCCGGTGAAGATTA+TGG	0.369872	1:+83149410	MsG0180004815.01.T01:CDS
AACCTTCAACGAAGTTGTCT+TGG	0.447834	1:+83148594	MsG0180004815.01.T01:CDS
AAGAGACTTGGATGACTTTG+TGG	0.405410	1:+83149439	MsG0180004815.01.T01:CDS
AAGAGTGTGTGCAGCAAATA+TGG	0.310178	1:+83147912	MsG0180004815.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATACACAGAAGAGAGAA+AGG	-	Chr1:83148465-83148484	None:intergenic	30.0%
AAAATTCCTGTAGGTTTGAA+GGG	+	Chr1:83148107-83148126	MsG0180004815.01.T01:intron	30.0%
AAATACACAGAAGAGAGAAA+GGG	-	Chr1:83148464-83148483	None:intergenic	30.0%
AAGATCGAGAAAATTACCAA+GGG	-	Chr1:83146277-83146296	None:intergenic	30.0%
AAGGAGATTAAGAATTGAAG+TGG	+	Chr1:83150250-83150269	MsG0180004815.01.T01:intron	30.0%
AATGTAGGTACCAAAACAAA+TGG	+	Chr1:83149197-83149216	MsG0180004815.01.T01:intron	30.0%
AATGTCAAACATTCATCACA+AGG	-	Chr1:83150457-83150476	None:intergenic	30.0%
ACAGGTATGCTTAAATGATA+CGG	+	Chr1:83144309-83144328	MsG0180004815.01.T01:intron	30.0%
ACATGTTTGTATAACTTCCA+AGG	+	Chr1:83148243-83148262	MsG0180004815.01.T01:intron	30.0%
ACATTGACATCAAAATTGCT+GGG	+	Chr1:83150472-83150491	MsG0180004815.01.T01:three_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	83144284	83150817	83144284	ID=MsG0180004815.01;Name=MsG0180004815.01
Chr1	mRNA	83144284	83150817	83144284	ID=MsG0180004815.01.T01;Parent=MsG0180004815.01;Name=MsG0180004815.01.T01;_AED=0.45;_eAED=0.51;_QI=0\|0.83\|0.76\|1\|0.75\|0.69\|13\|418\|440
Chr1	exon	83144284	83144312	83144284	ID=MsG0180004815.01.T01:exon:8840;Parent=MsG0180004815.01.T01
Chr1	exon	83144432	83144600	83144432	ID=MsG0180004815.01.T01:exon:8841;Parent=MsG0180004815.01.T01
Chr1	exon	83146080	83146279	83146080	ID=MsG0180004815.01.T01:exon:8842;Parent=MsG0180004815.01.T01
Chr1	exon	83147324	83147408	83147324	ID=MsG0180004815.01.T01:exon:8843;Parent=MsG0180004815.01.T01
Chr1	exon	83147894	83147991	83147894	ID=MsG0180004815.01.T01:exon:8844;Parent=MsG0180004815.01.T01
Chr1	exon	83148120	83148175	83148120	ID=MsG0180004815.01.T01:exon:8845;Parent=MsG0180004815.01.T01
Chr1	exon	83148539	83148656	83148539	ID=MsG0180004815.01.T01:exon:8846;Parent=MsG0180004815.01.T01
Chr1	exon	83148839	83148866	83148839	ID=MsG0180004815.01.T01:exon:8847;Parent=MsG0180004815.01.T01
Chr1	exon	83148970	83149061	83148970	ID=MsG0180004815.01.T01:exon:8848;Parent=MsG0180004815.01.T01
Chr1	exon	83149364	83149520	83149364	ID=MsG0180004815.01.T01:exon:8849;Parent=MsG0180004815.01.T01
Chr1	exon	83149693	83149817	83149693	ID=MsG0180004815.01.T01:exon:8850;Parent=MsG0180004815.01.T01
Chr1	exon	83149987	83150086	83149987	ID=MsG0180004815.01.T01:exon:8851;Parent=MsG0180004815.01.T01
Chr1	exon	83150334	83150817	83150334	ID=MsG0180004815.01.T01:exon:8852;Parent=MsG0180004815.01.T01
Chr1	CDS	83144284	83144312	83144284	ID=MsG0180004815.01.T01:cds;Parent=MsG0180004815.01.T01
Chr1	CDS	83144432	83144600	83144432	ID=MsG0180004815.01.T01:cds;Parent=MsG0180004815.01.T01
Chr1	CDS	83146080	83146279	83146080	ID=MsG0180004815.01.T01:cds;Parent=MsG0180004815.01.T01
Chr1	CDS	83147324	83147408	83147324	ID=MsG0180004815.01.T01:cds;Parent=MsG0180004815.01.T01
Chr1	CDS	83147894	83147991	83147894	ID=MsG0180004815.01.T01:cds;Parent=MsG0180004815.01.T01
Chr1	CDS	83148120	83148175	83148120	ID=MsG0180004815.01.T01:cds;Parent=MsG0180004815.01.T01
Chr1	CDS	83148539	83148656	83148539	ID=MsG0180004815.01.T01:cds;Parent=MsG0180004815.01.T01
Chr1	CDS	83148839	83148866	83148839	ID=MsG0180004815.01.T01:cds;Parent=MsG0180004815.01.T01
Chr1	CDS	83148970	83149061	83148970	ID=MsG0180004815.01.T01:cds;Parent=MsG0180004815.01.T01
Chr1	CDS	83149364	83149520	83149364	ID=MsG0180004815.01.T01:cds;Parent=MsG0180004815.01.T01
Chr1	CDS	83149693	83149817	83149693	ID=MsG0180004815.01.T01:cds;Parent=MsG0180004815.01.T01
Chr1	CDS	83149987	83150086	83149987	ID=MsG0180004815.01.T01:cds;Parent=MsG0180004815.01.T01
Chr1	CDS	83150334	83150399	83150334	ID=MsG0180004815.01.T01:cds;Parent=MsG0180004815.01.T01
Chr1	three_prime_UTR	83150400	83150817	83150400	ID=MsG0180004815.01.T01:three_prime_utr;Parent=MsG0180004815.01.T01

Gene Sequence

>MsG0180004815.01.T01

ATGTGCTCACAGCCGCGACTTGGAAACAGGGTTCTTAATGGGAAAGAGGAAATCCCAAATCTCTATGTGGGGAATATTATAAGGGGGATTCAGTATAGGGTGATTTTGTTAACACAATTGTACTTCAAATATGTGAGTAGAAAGGGTAATGGGGTAGCTCATAAGTTGCCCCAGTCTACTCTTATTGAGCACAATAACAAAGAAGAAGAGAGAAAGAAGAAGAAGAAGATGAAAATGGAGATGCATCAGATCTGGAGCAGAATAGCATTAGCATCGTTCGCGTTTGCAATTCTATTCGTATCAGTTTCAGCAGACGACGTGGTTGTGCTGACTGAAGAAAATTTCGAGAAGGAAGTTGGTCATGATAAAGGAGCTCTCGTTGAGTTCTACGCCCCTTGGTGTGGACACTGCAAAAAGCTTGCTCCGGAATATGAAAAGCTCGGTAACAGCTTCAAGAAAGCCAAATCTGTTTTGATTGCCAAGGTGGACTGTGATGAGCATAAGAGTGTGTGCAGCAAATATGGCGTCTCTGGGTACCCAACGATTCAGTGGTTTCCAAAAGGATCTCTAGAACCCAAAAAGTTTGAAGGGCCACGCACTGCAGAATCACTTGCTGAGTTTGTAAACACAGAAGGAGGAACCAATGTGAAGATTGCTACAGCTCCATCTCATGTAGTGGTGCTAACACCTGAAACCTTCAACGAAGTTGTCTTGGATGAAACCAAAGATGTCTTGGTTGAGTTTTATGCACCATGGTGTGGACATTGCAAAAGTCTTGCTCCTATTTATGAAAAAGTTGCTGCAGTTTTTAAATCGGAGGACGATGTAGTGATTGCAAATTTGGATGCCGACAAATATAGGGACCTGGCTGAAAAGTATGATGTAAGTGGATTTCCTACCTTGAAGTTCTTCCCAAAGGGCAACAAAGCCGGTGAAGATTATGGAGGTGGAAGAGACTTGGATGACTTTGTGGCTTTTATCAATGAGAAATCTGGAACAAGTCGAGATGCAAAAGGACAGCTTACTTCTGAGGCTGGTATAGTGGAAGACTTGGACGAATTGGTAAAGGAGTTTGTAGCTGCTGATGACAAAGAGAAGAAGGCCGTGTTTGCCCGAATTGAAGAGGAAGTTGAGAAGCTCAAGGGTTCTGCTTCTAGATACGGGAAGATTTACCTGAAAGTTTCTAAGAAGTACTTGGAGAAAGGTTCTGATTATGCTAAGAATGAAATTCAACGCCTAGAACGACTACTTGAAAAGTCTATCAGCCCTGCTAAGGCCGACGAATTGACCCTCAAGAAAAACATATTGTCTACATATGCTTGA

Protein sequence

>MsG0180004815.01.T01

MCSQPRLGNRVLNGKEEIPNLYVGNIIRGIQYRVILLTQLYFKYVSRKGNGVAHKLPQSTLIEHNNKEEERKKKKKMKMEMHQIWSRIALASFAFAILFVSVSADDVVVLTEENFEKEVGHDKGALVEFYAPWCGHCKKLAPEYEKLGNSFKKAKSVLIAKVDCDEHKSVCSKYGVSGYPTIQWFPKGSLEPKKFEGPRTAESLAEFVNTEGGTNVKIATAPSHVVVLTPETFNEVVLDETKDVLVEFYAPWCGHCKSLAPIYEKVAAVFKSEDDVVIANLDADKYRDLAEKYDVSGFPTLKFFPKGNKAGEDYGGGRDLDDFVAFINEKSGTSRDAKGQLTSEAGIVEDLDELVKEFVAADDKEKKAVFARIEEEVEKLKGSASRYGKIYLKVSKKYLEKGSDYAKNEIQRLERLLEKSISPAKADELTLKKNILSTYA*