Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004820.01.T01 | XP_024639376.1 | 92.079 | 101 | 1 | 1 | 13 | 113 | 310 | 403 | 1.25E-54 | 185 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004820.01.T01 | B7FIB9 | 91.089 | 101 | 2 | 1 | 13 | 113 | 39 | 132 | 6.00e-58 | 184 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000550.01 | MsG0180004820.01 | 0.811252 | 7.681561e-51 | 4.731687e-48 |
| MsG0180002482.01 | MsG0180004820.01 | 0.821334 | 4.267071e-53 | 3.461525e-50 |
| MsG0180004820.01 | MsG0280006407.01 | 0.805155 | 1.530333e-49 | 8.037716e-47 |
| MsG0180004820.01 | MsG0280007058.01 | -0.835241 | 1.882129e-56 | 2.288201e-53 |
| MsG0180004820.01 | MsG0480022795.01 | 0.840935 | 6.441875e-58 | 9.318449e-55 |
| MsG0180004820.01 | MsG0480023636.01 | 0.803725 | 3.040061e-49 | 1.538977e-46 |
| MsG0180004820.01 | MsG0780039844.01 | 0.804213 | 2.406025e-49 | 1.233370e-46 |
| MsG0180004820.01 | MsG0780040858.01 | 0.826191 | 3.105439e-54 | 2.892044e-51 |
| MsG0180004820.01 | MsG0780041507.01 | 0.829349 | 5.410622e-55 | 5.523969e-52 |
| MsG0180004820.01 | MsG0880041843.01 | 0.801884 | 7.293954e-49 | 3.523056e-46 |
| MsG0180004820.01 | MsG0880046126.01 | 0.822743 | 2.012259e-53 | 1.699019e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004820.01.T01 | MTR_1g087750 | 92.079 | 101 | 1 | 1 | 13 | 113 | 383 | 476 | 2.17e-58 | 186 |
| MsG0180004820.01.T01 | MTR_1g087750 | 92.079 | 101 | 1 | 1 | 13 | 113 | 432 | 525 | 4.44e-58 | 187 |
| MsG0180004820.01.T01 | MTR_7g102840 | 45.098 | 102 | 49 | 2 | 13 | 113 | 400 | 495 | 3.99e-19 | 81.6 |
| MsG0180004820.01.T01 | MTR_7g102870 | 43.137 | 102 | 51 | 2 | 13 | 113 | 402 | 497 | 5.04e-19 | 81.3 |
| MsG0180004820.01.T01 | MTR_7g112720 | 42.308 | 104 | 53 | 3 | 11 | 113 | 239 | 336 | 4.26e-15 | 69.7 |
| MsG0180004820.01.T01 | MTR_7g112720 | 42.308 | 104 | 53 | 3 | 11 | 113 | 370 | 467 | 7.08e-15 | 69.3 |
| MsG0180004820.01.T01 | MTR_7g406840 | 49.091 | 55 | 28 | 0 | 9 | 63 | 331 | 385 | 1.44e-13 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004820.01.T01 | AT5G27730 | 41.176 | 102 | 53 | 2 | 13 | 113 | 377 | 472 | 3.81e-16 | 73.2 |
| MsG0180004820.01.T01 | AT5G27730 | 41.176 | 102 | 53 | 2 | 13 | 113 | 296 | 391 | 6.65e-16 | 72.4 |
Find 24 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAACCAGCAATGCTTTCT+TGG | 0.234920 | 1:-83186910 | MsG0180004820.01.T01:three_prime_UTR |
| TCAAATTCATATACTGTTGC+AGG | 0.267323 | 1:-83187410 | MsG0180004820.01.T01:CDS |
| GGCCAGTGTACCCCACTTCT+CGG | 0.431670 | 1:+83187077 | None:intergenic |
| CAGTTATCCAGGGTTTCTAT+TGG | 0.432310 | 1:-83187288 | MsG0180004820.01.T01:CDS |
| GCTTGCCAAGAAAGCATTGC+TGG | 0.440971 | 1:+83186905 | None:intergenic |
| CAGCCAATGCATATACGATA+AGG | 0.452773 | 1:+83187329 | None:intergenic |
| GCTGGTTTCCTCCACAAGAA+AGG | 0.470862 | 1:-83187018 | MsG0180004820.01.T01:CDS |
| CTGGTTTCCTCCACAAGAAA+GGG | 0.472305 | 1:-83187017 | MsG0180004820.01.T01:CDS |
| GCAACAGTATATGAATTTGA+AGG | 0.477338 | 1:+83187413 | None:intergenic |
| TAGAAACCCTGGATAACTGC+TGG | 0.477768 | 1:+83187292 | None:intergenic |
| CGCCCTTATCGTATATGCAT+TGG | 0.492917 | 1:-83187332 | MsG0180004820.01.T01:CDS |
| AGCCAATGCATATACGATAA+GGG | 0.507849 | 1:+83187330 | None:intergenic |
| CAACAGTATATGAATTTGAA+GGG | 0.523186 | 1:+83187414 | None:intergenic |
| TGTATTACATTCCGAGAAGT+GGG | 0.529499 | 1:-83187088 | MsG0180004820.01.T01:CDS |
| TTATATATATCCCTTTCTTG+TGG | 0.530960 | 1:+83187007 | None:intergenic |
| GCGCTCTCCTGAAAATAACT+TGG | 0.533496 | 1:-83187266 | MsG0180004820.01.T01:CDS |
| ATGTATTACATTCCGAGAAG+TGG | 0.536676 | 1:-83187089 | MsG0180004820.01.T01:CDS |
| TATATATCCCTTTCTTGTGG+AGG | 0.552213 | 1:+83187010 | None:intergenic |
| TTCCGAGAAGTGGGGTACAC+TGG | 0.562880 | 1:-83187079 | MsG0180004820.01.T01:CDS |
| GAGAGCGCCAATAGAAACCC+TGG | 0.568447 | 1:+83187281 | None:intergenic |
| TAAAATCTCAATTATTACAA+AGG | 0.580860 | 1:+83187056 | None:intergenic |
| AGAAACCCTGGATAACTGCT+GGG | 0.607173 | 1:+83187293 | None:intergenic |
| ATCCATTGTAGTAAAACTGT+AGG | 0.607598 | 1:+83187364 | None:intergenic |
| GTATTACATTCCGAGAAGTG+GGG | 0.705016 | 1:-83187087 | MsG0180004820.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAATCTCAATTATTACAA+AGG | + | Chr1:83187281-83187300 | None:intergenic | 15.0% |
| !! | TAAATAAATTAATGTAACGT+TGG | + | Chr1:83187190-83187209 | None:intergenic | 15.0% |
| !!! | AATAATTGAGATTTTATTCT+GGG | - | Chr1:83187285-83187304 | MsG0180004820.01.T01:CDS | 15.0% |
| !!! | TAATAATTGAGATTTTATTC+TGG | - | Chr1:83187284-83187303 | MsG0180004820.01.T01:CDS | 15.0% |
| !! | CTGCTTAATTTATAAAGAAA+GGG | + | Chr1:83187128-83187147 | None:intergenic | 20.0% |
| !! | TAACAAATTTATTACCGAAT+AGG | - | Chr1:83187162-83187181 | MsG0180004820.01.T01:intron | 20.0% |
| !!! | ATAATTGAGATTTTATTCTG+GGG | - | Chr1:83187286-83187305 | MsG0180004820.01.T01:CDS | 20.0% |
| !!! | TATTCGGTAATAAATTTGTT+AGG | + | Chr1:83187163-83187182 | None:intergenic | 20.0% |
| ! | CAACAGTATATGAATTTGAA+GGG | + | Chr1:83186923-83186942 | None:intergenic | 25.0% |
| ! | CCTGCTTAATTTATAAAGAA+AGG | + | Chr1:83187129-83187148 | None:intergenic | 25.0% |
| ! | CCTTTCTTTATAAATTAAGC+AGG | - | Chr1:83187126-83187145 | MsG0180004820.01.T01:intron | 25.0% |
| ! | TTATATATATCCCTTTCTTG+TGG | + | Chr1:83187330-83187349 | None:intergenic | 25.0% |
| !! | ATTTTGTATTAAAGCTTCAC+TGG | + | Chr1:83187227-83187246 | None:intergenic | 25.0% |
| !!! | ACATTTACCAAGTTATTTTC+AGG | + | Chr1:83187078-83187097 | None:intergenic | 25.0% |
| !!! | GGTAAATGTTTATTTTCCAA+AGG | - | Chr1:83187089-83187108 | MsG0180004820.01.T01:CDS | 25.0% |
| ACATTAATTTATTTACGCGC+AGG | - | Chr1:83187195-83187214 | MsG0180004820.01.T01:intron | 30.0% | |
| ATCCATTGTAGTAAAACTGT+AGG | + | Chr1:83186973-83186992 | None:intergenic | 30.0% | |
| GCAACAGTATATGAATTTGA+AGG | + | Chr1:83186924-83186943 | None:intergenic | 30.0% | |
| TCAAATTCATATACTGTTGC+AGG | - | Chr1:83186924-83186943 | MsG0180004820.01.T01:three_prime_UTR | 30.0% | |
| ! | ACAGTTTTACTACAATGGAT+GGG | - | Chr1:83186973-83186992 | MsG0180004820.01.T01:three_prime_UTR | 30.0% |
| ! | TACAGTTTTACTACAATGGA+TGG | - | Chr1:83186972-83186991 | MsG0180004820.01.T01:three_prime_UTR | 30.0% |
| AGCCAATGCATATACGATAA+GGG | + | Chr1:83187007-83187026 | None:intergenic | 35.0% | |
| ATGTATTACATTCCGAGAAG+TGG | - | Chr1:83187245-83187264 | MsG0180004820.01.T01:intron | 35.0% | |
| GGGTAGATATAAAGAACCTT+TGG | + | Chr1:83187108-83187127 | None:intergenic | 35.0% | |
| TATATATCCCTTTCTTGTGG+AGG | + | Chr1:83187327-83187346 | None:intergenic | 35.0% | |
| TGTATTACATTCCGAGAAGT+GGG | - | Chr1:83187246-83187265 | MsG0180004820.01.T01:CDS | 35.0% | |
| ! | AGCCTACAGTTTTACTACAA+TGG | - | Chr1:83186968-83186987 | MsG0180004820.01.T01:three_prime_UTR | 35.0% |
| !!! | GATTTTATTCTGGGGTCTTT+TGG | - | Chr1:83187294-83187313 | MsG0180004820.01.T01:CDS | 35.0% |
| AGAAACCAGCAATGCTTTCT+TGG | - | Chr1:83187424-83187443 | MsG0180004820.01.T01:CDS | 40.0% | |
| CAGCCAATGCATATACGATA+AGG | + | Chr1:83187008-83187027 | None:intergenic | 40.0% | |
| CAGTTATCCAGGGTTTCTAT+TGG | - | Chr1:83187046-83187065 | MsG0180004820.01.T01:CDS | 40.0% | |
| GTATTACATTCCGAGAAGTG+GGG | - | Chr1:83187247-83187266 | MsG0180004820.01.T01:CDS | 40.0% | |
| ! | ATTTTCCCAGCAGTTATCCA+GGG | - | Chr1:83187036-83187055 | MsG0180004820.01.T01:CDS | 40.0% |
| !! | ATGTAACGTTGGCTCCTATT+CGG | + | Chr1:83187179-83187198 | None:intergenic | 40.0% |
| AGAAACCCTGGATAACTGCT+GGG | + | Chr1:83187044-83187063 | None:intergenic | 45.0% | |
| CGCCCTTATCGTATATGCAT+TGG | - | Chr1:83187002-83187021 | MsG0180004820.01.T01:CDS | 45.0% | |
| CTGGTTTCCTCCACAAGAAA+GGG | - | Chr1:83187317-83187336 | MsG0180004820.01.T01:CDS | 45.0% | |
| GCGCTCTCCTGAAAATAACT+TGG | - | Chr1:83187068-83187087 | MsG0180004820.01.T01:CDS | 45.0% | |
| TAGAAACCCTGGATAACTGC+TGG | + | Chr1:83187045-83187064 | None:intergenic | 45.0% | |
| ! | CATTTTCCCAGCAGTTATCC+AGG | - | Chr1:83187035-83187054 | MsG0180004820.01.T01:CDS | 45.0% |
| !!! | TTATTCTGGGGTCTTTTGGC+TGG | - | Chr1:83187298-83187317 | MsG0180004820.01.T01:CDS | 45.0% |
| ! | GCTGGTTTCCTCCACAAGAA+AGG | - | Chr1:83187316-83187335 | MsG0180004820.01.T01:CDS | 50.0% |
| TTCCGAGAAGTGGGGTACAC+TGG | - | Chr1:83187255-83187274 | MsG0180004820.01.T01:CDS | 55.0% | |
| !! | GAGAGCGCCAATAGAAACCC+TGG | + | Chr1:83187056-83187075 | None:intergenic | 55.0% |
| GGCCAGTGTACCCCACTTCT+CGG | + | Chr1:83187260-83187279 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 83186910 | 83187446 | 83186910 | ID=MsG0180004820.01;Name=MsG0180004820.01 |
| Chr1 | mRNA | 83186910 | 83187446 | 83186910 | ID=MsG0180004820.01.T01;Parent=MsG0180004820.01;Name=MsG0180004820.01.T01;_AED=0.48;_eAED=0.51;_QI=0|0|0.5|1|0|0|2|89|113 |
| Chr1 | exon | 83186910 | 83187139 | 83186910 | ID=MsG0180004820.01.T01:exon:27545;Parent=MsG0180004820.01.T01 |
| Chr1 | exon | 83187246 | 83187446 | 83187246 | ID=MsG0180004820.01.T01:exon:27544;Parent=MsG0180004820.01.T01 |
| Chr1 | CDS | 83187246 | 83187446 | 83187246 | ID=MsG0180004820.01.T01:cds;Parent=MsG0180004820.01.T01 |
| Chr1 | CDS | 83186999 | 83187139 | 83186999 | ID=MsG0180004820.01.T01:cds;Parent=MsG0180004820.01.T01 |
| Chr1 | three_prime_UTR | 83186910 | 83186998 | 83186910 | ID=MsG0180004820.01.T01:three_prime_utr;Parent=MsG0180004820.01.T01 |
| Gene Sequence |
| Protein sequence |
